KR20030015424A - The physiofunctional fermented liquor with purple sweet potato and the producing method of therof - Google Patents

Abstract

PURPOSE: Fermented wine using purple sweet potatoes and a preparation process thereof are provided, thereby preparing high quality of fermented wine having improved thrombolytic activity and tyrosinase-inhibiting activity. CONSTITUTION: The process for preparing fermented wine using purple sweet potatoes comprises the steps of: pulverizing nonglutinous rice, inserting the pulverized rice into the boiling water and cooling it; adding malt and wheat flour into the rice solution; inoculating Saccharomyces cerevisiae cultured in a YEPD medium into the mixture; culturing it at 30 deg. C for 2 days to prepare crude liquor; soaking nonglutinous rice and glutinous rice, then followed by removing water therefrom, steaming in a pot at 100 deg. C for 1 hour and cooling them; inserting 5 to 15 wt.% of steamed purple sweet potatoes, 8 to 12 wt.% of malt and 8 to 12 wt.% of the crude liquor into the steamed rice mixture; adding water into the mixture and fermenting it at 23 to 27 deg. C for 9 to 15 days; and filtering the fermented mixture.

Description

Physiologically functional fermented liquor using purple sweet potato and its manufacturing method {THE PHYSIOFUNCTIONAL FERMENTED LIQUOR WITH PURPLE SWEET POTATO AND THE PRODUCING METHOD OF THEROF}

The present invention relates to a physiologically functional fermented liquor using a purple sweet potato and a method of manufacturing the same.

Sweet potato is a food rich in fiber protein and minerals, etc., but the development of processed food is limited because it has a characteristic smell of sweet potato and has a poor shelf life.

In particular, the purple sweet potato has less than 1/3 of the free sugar content compared to the general yellow sweet potato (yellow rice, Yulmi, etc.), and when used as a steamed sweet potato or roasted sweet potato, the sugar content is low and the purple pigment has a high dissolution rate. .

In addition, purple sweet potatoes are difficult to be glycosylated by starch dehydrogenase, which is difficult to develop a variety of processed foods.

However, since purple sweet potatoes contain natural pigments and a large amount of functional substances, anthocyanin, new food processing methods using them are required.

In Korean Patent Application No. 10-1999-0053047 (Lactobacillus fermented food production technology using purple sweet potato) filed by the applicant of the present invention, it is known that fermentation of purple sweet potato using lactic acid bacteria such as Lactobacillus vulgaris is known. Since the present invention relates to the production of fermented liquor by alcoholic fermentation using yeast, the object and technical configuration are different.

On the other hand, Korean Patent Application No. 1994-20343 (method of producing sweet potato red wine using purple sweet potato) discloses the content of making red wine by mixing and fermenting normal sweet potato and purple sweet potato in a ratio of 5: 5. The composition of technology, raw materials, fermentation methods, etc., the composition of the technology is different, the physiological functional effect is also unclear.

In addition, the patent application, but the contents of making a red wine by mixing and fermenting grains and purple sweet potatoes in a ratio of 5: 5, but the present invention in the manufacturing method, parallel double fermentation, mixing ratio of materials, fermentation conditions, etc. There are many differences and the physiological effects are also unclear.

In addition, Japanese Laid-Open Patent Publication No. 6-113810 (Manufacturing method of red liquor made from purple sweet potatoes) discloses the content of making purple liquor by adding organic acid to the addition of purple sweet potatoes and adjusting the pH, and adding and fermenting yeast. The present invention is different from the present invention in the manufacturing method, raw material, fermentation method, etc. of the main hair, and the physiological functional effect is also unclear.

The present invention has a weak shelf life and difficult to break down sugars, but using a purple sweet potato containing a large amount of anthocyanin, a functional pigment, a physiologically functional fermented liquor using a purple sweet potato having excellent taste and taste and high color and physiological functional effects. It is to provide a manufacturing method.

1 is a manufacturing process chart of the immersion cast iron of the present invention

Figure 2 is a manufacturing process of the physiological functional fermented wine using the purple sweet potato of the present invention

Figure 3 is a taste component sensory test of the present invention fermented wine

Figure 4 is a taste component sensory test of the present invention fermented wine.

The present invention relates to a physiologically functional fermented liquor using a purple sweet potato and a method of manufacturing the same.

Fermented sake of the present invention, by cultivating the addition of flour and malt and yeast in the steamed rice, to make immersion Joju, add a certain amount of purple purple sweet potato to the cooked glutinous rice and non-glutinous rice, immersion cast Prepared by parallel fermentation by adding water and then filtered.

A method of preparing a physiologically functional fermented wine using the purple sweet potato according to the present invention will be described with reference to FIGS. 1 and 2.

(1) Preparation of immersion cast wool

After heating into the crushed rice in boiling water as cooling, the addition of 18-22% by weight malt and 8-12% by weight of wheat flour and process three Levy MY jiae (Saccharomyces cerevisiae) with the yeast Saccharomyces culture in YEPD medium uniform Mix, and incubate at 27-33 ° C. for 2 days to form immersion cast.

(2) Preparation of Physiologically Functional Fermented Wine Using Purple Sweet Potato

To each of rice and glutinous rice immersed in water and cooked, add 5-15% by weight of cooked purple sweet potato, add 8-12% by weight of yeast and 8-12% by weight of the soaked cast granules made above, and add water. Next, parallel fermentation for 9-15 days at a temperature of 23-27 ℃, and then filtered to prepare a physiologically functional fermented wine using the purple sweet potato of the present invention.

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples, and Experimental Examples, but these examples do not limit the scope of the present invention.

<Example 1> Preparation of immersion cast hair

Non-rice was used in Seocheon, Chungnam.

Nuruk used the product of the central grain.

As the yeast for producing hair, conventional Saccharomyces cerevisiae was used.

Non-rice was milled to 35 mesh.

400 g of ground non-glutinous rice was put in 500 ml of boiling water, heated, and cooled.

50 g of wheat flour and 100 g of yeast were added thereto.

A conventional yeast Saccharomyces cerevisiae was incubated at 30 ° C. for 2 days in YEPD medium, and 100 ml of this culture solution was added and mixed uniformly.

This mixture was incubated at 30 ° C. for 2 days to prepare immersion cast iron.

Example 2 Preparation of Fermented Wine Using Purple Sweet Potato

Non-glutinous rice and glutinous rice were produced from Seocheon, Chungnam.

The purple sweet potatoes were grown in Chungnam Agricultural Research and Development Institute. The chemical components were 23.8% of total sugar, 1.40% of crude protein, 1.19% of crude fat, 1.37% of ash, and 71.34% of moisture.

Nuruk used the product of the central grain.

500 g of non-glutinous rice and glutinous rice were immersed in water for 16 hours, and then water was removed.

The soaked non-glutinous rice and glutinous rice were each steamed at 100 ° C. in a high pressure steamer for 1 hour, then cooled and placed in a fermenter.

100 g of purple sweet potato was added to 100 ° C. in a high pressure steam cooker for 1 hour.

100 g of purple sweet potato, 100 g of yeast, and 100 g of immersion cast hair prepared in Example 1 were placed in a fermenter, and 2,400 ml of water was added thereto.

Parallel fermentation was carried out for 20 days while maintaining the temperature of the fermenter at 25 ° C.

The fermented product was filtered to prepare a physiologically functional fermented wine using the purple sweet potato of the present invention.

<Comparative Example 1> Preparation of fermented wine with different amount of purple sweet potato added and fermentation period

The other conditions, such as the preparation of the main hair, fermentation temperature, and the like as in Examples 1 and 2, but the fermented liquor was prepared by changing the addition amount and the fermentation period of the purple purple sweet potato.

5% by weight of cooked purple sweet potato, 10% by weight, 15% by weight, 20% by weight, 50% by weight, and 70% by weight were prepared.

For each fermented wine prepared alcohol content, pH, pigment amount, and the like, the results are summarized in Table 1.

<Table 1> Characteristics of Main Components of Fermented Wine by Addition of Purple Sweet Potato and Fermentation Period

Addition amount (%) Effective date Ethanol (%) pH Total acid (%) Volatile Acid (%) Pigment amount (mg / ml) 5 5 13.0 4.11 0.24 0.0047 0.44 10 15.6 4.31 0.25 0.0053 0.54 15 17.0 4.54 0.26 0.0082 0.40 10 5 12.8 4.20 0.25 0.0118 0.83 10 16.8 4.31 0.26 0.0065 0.74 15 17.0 4.53 0.27 0.0082 0.48 20 5 14.0 4.20 0.25 0.0047 1.20 10 16.0 4.42 0.25 0.0091 1.25 15 16.4 4.52 0.27 0.0129 1.10 50 10 15.4 4.21 0.27 0.0083 3.60 70 10 14.6 4.23 0.30 0.0124 4.50 No addition 10 8.8 3.82 0.26 0 0

Ethanol production amount of 5% by weight of purple sweet potato was added, 17.0% at 15 days of fermentation, 10% by weight, 16.8% at 10 days of fermentation, and 17.0% at 15 days of fermentation.

When the added amount of purple sweet potato was increased to 20 wt%, 50 wt%, and 70 wt%, the initial ethanol production concentration was high, but the final ethanol production was not significantly changed or rather decreased.

pH was 4.11-4.53, and even if the addition amount changed, the effect on pH was small.

The amount of pigment increased as the amount of purple sweet potato increased.

<Comparative Experiment 1> Comparative sensory test of fermented wine using purple sweet potato

The sensory test was carried out for the fermented liquor using the purple sweet potatoes prepared in Example 1 and Comparative Example 1.

Requested by 30 trained sensory evaluation members from the Graduate School of Bae Jae University, Korea Ginseng and Tobacco Research Institute and Chungnam Agricultural Research and Development Institute, QD (Quanttitative Descriptive Analysis) was performed as follows.

Have sensory evaluation members describe the characteristics of flavor and taste that can be felt in purple sweet potato fermented liquor, average the value of 1 as the most dislike, 9 as the best, and analyze variance and Duncan's multi-range The significance between samples was analyzed.

As a result, Fig. 3 shows the flavor and Fig. 4 shows the flavor.

The more sweet potato was added, the more the sweet potato smelled.

The more sweet potato, the more sweetness and sour taste, the less bitterness and the peculiar smell of fermented liquor.

The characteristics of flavor and taste affected the preference, so that the overall preference was the highest in 10 wt% of purple sweet potato, followed by 5 wt%, 20 wt%, 50 wt%, and 70 wt% fermentation. .

<Comparative Experiment 2> Comparison of Colors of Fermented Wine without Addition of Purple Sweet Potato

The chromaticity of each of the fermented wines of Example 1 and Comparative Example 1 prepared by adding purple sweet potato and the fermented wine prepared without addition was compared, and the results are shown in Table 2.

Chromaticity was measured with a color difference meter (Color Difference Meter D-25L-9, Hunter Associotes Lab. Inc. USA) and expressed as L value (brightness), a value (redness), and b value (yellowness).

<Table 2> Color of Fermented Wine by Addition of Purple Sweet Potato

Added amount (% by weight) L (明度, brightness) a (red) b (yellow) 5 55.39 14.48 6.30 10 48.11 20.92 6.19 20 42.16 22.74 5.63 50 37.31 21.26 5.86 70 15.51 45.26 15.32 No addition 79.59 -1.86 19.75

As shown in Table 2, L value (brightness) and b value (yellowness) decreased as the amount of purple sweet potato increased, and a value (redness) increased.

Comprehensive analysis of the results of Comparative Experiments 1 and 2 above, fermented stocks prepared by fermenting 5-15% by weight of purple sweet potato compared to non-glutinous rice and glutinous rice at 23-27 ℃ for 9-15 days I think it is excellent.

Experimental Example 2 Comparative Experiment of Physiological Functional Effects of Purple Sweet Potato Fermented Wine.

The fermented wine of Example 1, which is considered to have high preference and excellent economic efficiency, was used as a sample, and a physiological functional effect comparison experiment was conducted for commercially available dandelion fermented wine and general rice fermented wine.

As a reagent for measuring physiological function, Sigma, USA or a special analytical reagent was used.

(1) Comparative measurement of thrombolytic activity

500 µl of the fermented liquor concentrate prepared in Example 1 was added to 3 ml of a fibrin solution (0.6%) and reacted at 40 ° C for 10 minutes, and then 3 ml of 0.4 M TCA solution was added to stop the reaction and filtered.

The amount of tyrosine tyrosine in the filtrate was quantified by the development of 1 N folin reagent. At this time, 1 unit of enzyme was used to produce 1 µg of tyrosine for 1 minute.

In the same way, the measurement of dandelion fermented liquor and general rice fermented liquor was performed.

(2) Measurement of Tyrosinase Inhibitory Activity

The fermented wine prepared in Example 1 was used as a sample.

To 500 μl of sample concentrate, 0.2 ml of 5 mM L-DOPA and 0.1 M sodium phosphate buffer (pH 6.0) were mixed, followed by addition of tyrosinase 11 U and reaction at 35 ° C. for 2 minutes. Next, the absorbance was measured at 475 nm and the activity was calculated by comparison with the value of the sample-free control.

Experimental measurements were made for dandelion fermented liquor and normal rice fermented liquor in the same way.

Table 3 shows the results of the experimental measurements as described above.

<Table 3> Comparison results of physiological functional effects of fermented wine

division Thrombolytic activity (U) Tyrosinase Inhibition (%) Purple sweet potato fermented liquor 20.0 97.0 Dandelion fermented wine 18.7 96.9 Fermented rice 5.6 75.5

As a result of the measurement of physiological function, the purple sweet potato fermented strain of the present invention exhibited the best 20.0 U in thrombolytic activity.

High thrombolytic activity helps prevent high blood pressure.

In addition, tyrosinase inhibitory activity was also greatest in the effect of the purple sweet potato fermentation strain of the present invention was 97.0%.

Tyrosinase inhibitory effect is known to be good for skin whitening.

The present invention provides a physiologically functional fermented liquor and a method for producing the same, which are excellent in aroma and taste using purple sweet potato, have a high thrombolytic activity, have a high tyrosinase inhibitory effect, and are effective for preventing hypertension and whitening.

Claims (3)

By uniformly mixing the crushed and cooled was introduced into the heating in boiling water the rice and then yeast and adding flour and 2 days aged My process as a culture of yeast Saccharomyces Levy jiae (Saccharomyces cerevisiae) at 30 ℃ in YEPD medium 30 Incubated for 2 days at ℃ to prepare a immersion cast After immersing non-glutinous rice and glutinous rice in water, the water was removed, and the mixture was cooled by increasing the temperature at 100 ℃ for 1 hour in a high-pressure steamer, and 5-15% by weight purple sweet potato and 8-12% by yeast and prepared immersion 8-12% by weight of hair seedling is added thereto, and water is added thereto, followed by parallel fermentation for 9-15 days in a 23-27 ° C. fermentation tank, followed by filtration, to prepare a physiologically functional fermented wine using purple sweet potato. Physiologically functional fermented liquor using purple sweet potatoes prepared by the method of claim 1. The method according to claim 1, wherein the purple purple sweet potato is cooked at 100 ° C. for 1 hour.