KR20030014007A - Health assistant foodstuffs comprising of functional chitosan-lactic salts and preparing method thereof - Google Patents

Abstract

PURPOSE: A functional chitosan lactate food-supplement and a production method thereof are provided, thereby producing the food-supplement which has certain ranges of deacetylation degree and molecular weight, and is thus useful for prevention and treatment of adult diseases. CONSTITUTION: The functional chitosan lactate food-supplement is composed of chitosan lactates having deacetylation degree of 60 to 90% and an average molecular weight of 160,000 to 600,000, wherein the chitosan lactates comprise 0.1 to 19 wt.% of chitosan lactate having an average molecular weight of 100,000 to 200,000, 80 to 99.9% of chitosan lactate having an average molecular weight of 250,000 to 450,000, and 1 wt.% or less of chitosan lactate having an average molecular weight of 5,000. The method for producing the functional chitosan lactate food-supplement comprises the steps of: dispersing chitosan having deacetylation degree of 60 to 90% in water; dissolving the dispersed chitosan in a lactic acid solvent and digesting it with chitosanase; separating the digested chitosan lactates according to their molecular weights and drying them; and mixing the separated chitosan lactates in a certain ratio.

Description

Functional Chitosan Lactate Health Supplements and Manufacturing Method Thereof {HEALTH ASSISTANT FOODSTUFFS COMPRISING OF FUNCTIONAL CHITOSAN-LACTIC SALTS AND PREPARING METHOD THEREOF}

The present invention relates to a functional chitosan lactate dietary supplement and a method of manufacturing the same, and more particularly, to a health supplement food consisting of chitosan lactate having a specific degree of acetylation and a specific acetylation degree for improving the physiological activity function and its It relates to a manufacturing method.

Shells of crustaceans, such as crabs and shrimp, produced by the food industry, contain about 20% natural polysaccharides per unit weight. This polysaccharide is a natural polymeric material produced 100 billion tons per year on Earth and broken down again by living things. Chitosan, mainly extracted from the shell of crustaceans, has a similar chemical structure to cellulose. Chitosan is a polymer in which glucosamine, a unit component, has β-1,4 bonds with adjacent glucosamine. Therefore, it is excreted undegraded by enzymes that cleave α-1,4 bonds and α-1,6 bonds secreted from the digestive tract in the body.

Formula 1 and Formula 2 below represent the chemical structures of chitosan and cellulose, respectively.

Chitosan represented by the above formula (1) is a material with high biocompatibility and little antigenicity, and is not only a readily available material but also can be used as a substitute for plant dietary fiber such as cellulose because it is not degraded by normal enzymes in the digestive organs. have.

Today, as environmental pollution increases due to the development of civilization and natural destruction, there are more opportunities for exposure to harmful substances such as heavy metals and dioxins, and the development and consumption of processed foods increases the high-calorie foods and harmful food additives. Ingestion of a large amount of this, it can be easily identified from the surrounding many cases of incurable diseases such as various adult diseases (food bottles) and cancer. In particular, the problem of diet is very serious as it is directly related to the disease. As the standard of living increases, the diet is shifted to meat rather than vegetables, and the preference for instant foods is causing overeating, obesity, overnutrition, and nutritional imbalances. Due to such changes in the eating environment and environmental pollution, the necessity of artificially controlling the balance of the human body's immunity, nutritional status, and body rhythm is increasing. In order to maintain life, the absorption and excretion of the nutrients is most fundamentally necessary. These processes can directly or indirectly cause the disease, prevent the disease, or cure the disease. Carbohydrates, fats, proteins, vitamins, and minerals are essential for humans, and fiber is essential for the absorption and excretion of these nutrients. Previous studies have shown that dietary fiber deficiency can cause colorectal cancer, constipation, heart disease, appendicitis, high blood pressure, urethral stones, diabetes, hyperlipidemia, or gallstones. Chitosan, derived from crustacean shells, is a fiber with a structure similar to the cellulose that forms the cell walls of plants, and has been reported by various chitosan scholars to excrete all wastes in the body and activate individual tissue cells.

Chitosan has been reported to be effective in eliminating cholesterol and fat adsorption in food, stool removal in the large intestine, diet, hangover removal, excreting alcohol adsorption, improving liver function, and various clinical trials have been reported. However, the molecular weight range of the chitosan, the content ratio of the composition, and the degree of deacetylation of the chitosan are not specifically specified, and most of them are only described by the term chitosan or chitosan oligosaccharide. Chitosan can be subdivided almost infinitely according to its deacetylation degree, viscosity, molecular weight, and the like, and since they are insoluble in water, pure chitosan alone is not dissolved in the stomach and is known to have little effect.

Korean Patent Laid-Open Publication No. 1998-10384 (98.6.6.) Discloses the conversion of acetaldehyde, a typical hangover substance, into a complex compound using chitosan to prevent hangovers. Although it is proposed a functional shochu using chitosan that can inhibit the proliferation of Helicobactor pylori and reduce the stomach soreness due to gastric acid neutralization effect, chitosan does not have the ability to adsorb alcohol and acetaldehyde in vitro. However, since chitosan is not absorbed into the body, there is a problem in that the acetaldehyde generated in blood vessels cannot be adsorbed and excreted, and the physical properties of chitosan used are not disclosed at all.

Korean Patent Publication No. 98-142373 (98.6.1. Announcement) and Korean Patent Publication No. 2000-63528 (Published Jan. 1, 2000) respectively include direct symptoms such as hangover, indigestion, diarrhea, fatigue, fatty liver, diabetes, Alcohol preventive and preventive agents for preventing and improving complex indirect symptoms such as vascular diseases and alcohol and beverages containing low molecular weight chitosan have been proposed, but the use of chitosan oligosaccharides is not related to the use of chitosan.

Korean Patent Publication No. 2000-24835 (published on May 5, 2000) proposes a safe nutritional composition without side effects added to animal feed for the purpose of promoting livestock growth, improving feed efficiency, and increasing anti-medical properties against various diseases. This is an organic synergy of chitosan oligosaccharides and vitamins and is not directly related to chitosan.

Korean Patent Publication No. 1998-67728 (published on Oct. 15, 1998) proposes a method for removing E. coli, harmful bacteria, and heavy metals containing health foods using chitin and chitosan derivatives, which cannot be boiled or disinfected. It is used to remove E. coli such as green juice, vegetables, or fruit juice, or harmful bacteria, heavy metals, pesticides, or preservatives. It's not about.

In addition, Korean Patent Publication No. 2001-18321 (published on March 3, 2001) proposes a health food composition for rheumatoid arthritis patients containing 20-95% chitosan oligosaccharides containing 5-80% glucosamine and chondroitin sulfate as a snail extract. Doing.

Korean Patent Laid-Open No. 2001-44175 (published on June 6, 2001) proposes a method for inhibiting the synthesis and secretion of a cell active material using chitosan and a chitosan-containing food composition for inhibiting the method, which has a molecular weight of 10,000 to 10,000. It is described as inhibiting the synthesis and secretion of alcohol-mediated cell activator of hepatocytes using a polymer water-soluble chitosan of 1,500,000, the food composition comprises 0.01 to 90% by weight of a polymer water-soluble chitosan having a molecular weight of 10,000 to 1,500,000 It is described as. However, the chitosan within the above molecular weight range composed of β-1,4 bonds does not decompose in the digestive tract, so it is not absorbed into the blood vessels, and thus the transition to the liver is impossible.

Accordingly, an object of the present invention is to provide a specific degree of acetylation of different molecular weights for the purpose of improving the biological activity such as cholesterol and fat adsorption excretion in food, stool removal in the large intestine, diet, hangover removal, alcohol adsorption excretion, liver function improvement, etc. It is to provide a functional chitosan lactate health supplement foods having chitosan lactate having a specific composition ratio.

Another object of the present invention is to provide a method for producing a functional chitosan lactate health supplement according to the above object.

According to one preferred aspect of the present invention for achieving the first object of the present invention, the deacetylation degree of chitosan lactate has a range of 60 to 90%, number average molecular weight of 160,000 to 600,000 Functional chitosan lactate dietary supplement is provided.

According to another preferred aspect of the present invention for achieving the first object of the present invention described above, 0.1 to chitosan lactate having a deacetylation degree in the range of 60 to 90% and a number average molecular weight of 100,000 to 200,000. A functional chitosan lactate dietary supplement is provided which is comprised of 20 wt%, 80 to 99.9% of chitosan lactate having a number average molecular weight of 250,000 to 450,000, and less than 1 wt% of chitosan lactate having a number average molecular weight of less than 5,000.

According to one preferred aspect of the present invention for achieving the second object of the present invention, the chitosan swelling step of dispersing chitosan in the range of 60 to 90% deacetylation in water (chitosan concentration 1-12%), swelling Dissolved chitosan in lactic acid solvent (lactic acid concentration 1-5%), followed by enzymatic digestion with chitosanase, separation and drying of the decomposed chitosan lactate by molecular weight, and chitosan lactate separated by molecular weight at a predetermined ratio. Provided is a method for preparing a functional chitosan lactate dietary supplement consisting of mixing.

Hereinafter, the present invention will be described in more detail.

The chitosan lactic acid health supplement for improving human physiological activity according to the present invention is composed of chitosan having a certain degree of deacetylation, purified by molecular weight and mixed at a predetermined ratio, and the chemical property of the raw material chitosan is an important factor in indicating physiological activity.

Functional chitosan lactic acid dietary supplement of the present invention, chitosan, which has been reported to have physiological activity enhancing effects such as elimination of cholesterol and fat adsorption in food, elimination of stool in large intestine, diet, elimination of alcohol, excretion of alcohol, improvement of liver function, etc. It is a dietary supplement consisting of chitosan lactate powder adjusted to have a specific molecular weight distribution and degree of deacetylation.

The deacetylation degree of chitosan constituting the chitosan lactic acid health supplement of the present invention is in the range of 60 to 90%, the number average molecular weight is composed of chitosan lactic acid in the range of 160,000 to 600,000, preferably, the number average molecular weight 100,000 It is composed of 0.1 to 20% by weight of chitosan lactate of -200,000 and 80 to 99.9% of chitosan lactate of 250,000 to 450,000, and may optionally further comprise less than 1% by weight of chitosan lactate having a number average molecular weight of less than 5,000.

Chitosan with a number average molecular weight of 2,000 or less is reported to be absorbed in the digestive tract and exhibits immune enhancing effects. Chitosan lactic acid with a number average molecular weight of 160,000 to 600,000 is excreted without being absorbed into the blood vessels in the digestive tract, It has been reported to have a physiological activity enhancing effect in the removal of cholesterol and fat adsorption in the large intestine, elimination of stool in the large intestine, diet, elimination of hangover, excretion of alcohol adsorption, liver function. In particular, the composition comprises 0.1-20% by weight of chitosan lactate having a number average molecular weight of 100,000-200,000 and 80-99.9% of chitosan lactate having a number average molecular weight of 250,000-450,000, and optionally less than 1% by weight of chitosan lactate having a number average molecular weight of less than 5,000. Inclusion of further enhances the effect as described above clinically. In addition, the deacetylation degree of chitosan is 60 to 90%, and the chitosan within this range has the highest immune resiliency.

In the present invention, since the chitosan needs to be made water-soluble, it is prepared in the form of chitosan lactate, and the preparation method is a chitosan swelling step of dispersing chitosan having a deacetylation degree in the range of 60 to 90% in water and swelling. The chitosan was dissolved in a lactic acid solvent and then enzymatically decomposed with a chitosanase, separating and drying the decomposed chitosan lactate by molecular weight, and mixing the chitosan lactate separated by molecular weight in a predetermined ratio.

Hereinafter, the present invention will be described in more detail with reference to Examples.

Example 1 Selection of Chitosan

The deacetylation degree of chitosan used the thing of 60 to 90%. Raw material chitosan was purchased commercially available chitosan, and subjected to a comprehensive evaluation using PVSK titration, acid-alkali titration, and infrared spectrophotometer, and then deacetylation degree of chitosan was determined. Chitosan which evaluated the degree of deacetylation here is chitosan of the number average molecular weights 160,000-200,000 (henceforth abbreviated as sample "A"), and 200,000-600,000 (henceforth abbreviated as "B").

Example 2: Decomposition of Chitosan

After swelling the chitosan sample 'A' in Example 1 in warm water, the swollen chitosan sample 'A' was dissolved in a lactic acid solvent, and chitosanase (Meiji Seika Co., Japan) was added thereto. Enzymatic digestion gave chitosan digest.

Example 3: Separation of Chitosan Degradate by Molecular Weight

Chitosan digests prepared in Example 2 were separated for each molecular weight using the solvents shown in Table 1 below.

Mixing ratio of the separation solvent of each chitosan decomposition product division Precipitated solvent Mixing ratio Supernatant precipitate Molecular Weight Cut-Off Range Group 1 Degradation products / ethanol 1: 3 Supernatant 1 (Separation process for Group 2) Dialysis Process 15,000 Group2 Supernatant 1 / ethanol 1: 5 Supernatant 2 (Separation Process for Group 3) Dialysis Process 15,000 Group3 Supernatant 2 / ethanol 1: 7 Supernatant 2 (Separation Process for Group 4) Dialysis Process 3,500 Group 4 Supernatant 3 / ethanol 1:10 Gel Filtration Process After Concentration Dialysis Process 3,500

Groups 1 and 2 were dialyzed in a dialysis membrane with a number average molecular weight cut-off range of 15,000, and groups 3 and 4 with a number average molecular weight cut-off range of 3,500. Pure water was used for the water outside the dialysis membrane, and the separation was performed for each molecular weight using dialysis membrane dialysis and gel filtration.

Example 4: number average molecular weight evaluation

All samples were dissolved in water and chitosan samples 'A' and 'B' were lactic acid to evaluate the molecular weight of each group sample and chitosan samples 'A' and 'B' separated by gel filtration and dialysis in Example 3. The number average molecular weight was analyzed by dissolving in buffer (pH 5.5). The analytical instrument was evaluated by gel permeation chromatography and light scattering method.The standard sample was Pullulan, the column was Asahipak GFA series, the eluent flow rate was 0.3ml / min, and the eluent was 0.2M acetic acid buffer solution ( pH 5.5), the sample was detected in the eluent using a Hitachi L-3300 Refractive-index detecter (Japan). The analysis was performed at an analysis temperature of 50 ° C. and the analysis results are shown in Table 2 below.

Example 5: Evaluation of pKa by Number Average Molecular Weight Distribution

500mg of each sample was collected and dissolved in 50ml of 0.1N HCl (containing 0.1M NaCl) and supplied with 0.1N NaOH (containing 0.1N NaCl) solution under the supply of nitrogen gas. Ltd., Auto Titrator, and COM-500 (Japan) were titrated at room temperature to obtain pKa values for each number average molecular weight distribution. The results are shown in Table 2 below.

Number average molecular weight distribution and pKa Fraction Number average molecular weight Molecular weight distribution pKas One 20,000 1.10 6.3 2 16,000 1.05 6.4 3 11,100 1.15 6.4 4 9,500 1.10 6.4 5 8,200 1.20 6.5 6 7,000 1.10 6.5 7 6,000 1.10 6.6 8 5,500 1.05 6.8 9 4,100 1.10 6.8 10 3,000 1.10 6.8 11 2,500 1.10 6.8 12 2,000 1.05 7.2 13 1,100 1.20 7.2 Chitosan A 160,000 1.45 6.2 Chitosan B 320,000 1.50 6.1

Example 6: chitosan with a number average molecular weight of 2,000 or less

In Table 2, chitosan having a number average molecular weight of 2,000 or less is contained from monosaccharides, disaccharides to 11 sugars, and 1-6 glycated sugars are absorbed into the blood vessels in the small intestine to show biological immune activity. These sugars were molecular weight controlled by enzymatic digestion and separated by molecular weight according to the same method as described in the method of Example 3.

Example 7: Chitosan Lactic Acid with Number Average Molecular Weight 100,000-200,000 (especially 160,000)

It has been clinically confirmed to excrete fat, cholesterol, anionic components (fatty acid, bile acid, etc.), alcohol, etc. from food, remove stool, prevent constipation, and improve constipation. Chitosan having a number average molecular weight in the above-mentioned range was dispersed and swollen in powdered chitosan at 8% concentration in hot water, and then adjusted to 3% lactic acid concentration and dissolved under stirring. After dissolution, it was filtered, lyophilized and triturated and then sterilized.

Example 8: Chitosan Lactic Acid with Number Average Molecular Weight 250,000-450,000 (particularly 320,000)

Chitosan in the above range is effective in the action of fat, cholesterol excretion and alcohol adsorption excretion in the food, but it has been clinically confirmed to cause constipation when a lot of this component. The powdery chitosan was dispersed and swollen in hot water to an 8% concentration, adjusted to a lactic acid concentration of 3% and dissolved under stirring. After dissolution, it was filtered, lyophilized and triturated and then sterilized.

Example 9 Preparation of Chitosan Lactic Acid Mixed Powder

Chitosan lactic acid having various ranges of number average molecular weights prepared according to the above-described embodiments was prepared at various mixing ratios, and clinical experiments were conducted. As a result, it was found to be most effective at the following mixing ratios.

Mixing ratio of chitosan lactate Ingredient Name Content (mg) Compounding ratio (% by weight) Number average molecular weight Ultra High Molecular Weight Chitosan Lactate 190 0.1-20 100,000-200,000 Polymeric Chitosan Lactate 800 80 to 99.9 250,000-450,000 Ultra low molecular weight chitosan 10 0 to 1 2,000 or less system 1,000 100

As described above, the present invention mixes chitosan lactic acid salt having a specific range of deacetylation degree and molecular weight range with a specific composition ratio by oral chitosan health supplement food, thereby optimizing and improving physiological activity function. Specifically, considering that pKa of chitosan is different according to the molecular weight range and its bioactive properties may be different, specific deacetylases combining the molecular weight range showing the most effective physiological activity as well as expressing effective physiological activity are expressed. By selecting chitosan having a degree of purity and making it into a lactate form to effectively soluble in the body, many adult diseases, unlike the dietary supplement made of chitosan oligosaccharides, which have less conventional effects and can cause side effects such as congestion, The prevention, improvement, or treatment can reduce the medical expenditures of the public and reduce the financial burden of medical insurance, which is a national problem.

Claims (3)

A functional chitosan lactate health supplement comprising a chitosan lactate having a deacetylation degree in a range of 60 to 90% and a number average molecular weight in a range of 160,000 to 600,000. The chitosan lactate according to claim 1, wherein the chitosan lactate is 0.1 to 19% by weight of chitosan lactate having a number average molecular weight of 100,000 to 200,000, 80 to 99.9% of chitosan lactate having a number average molecular weight of 250,000 to 450,000, and 1% by weight of chitosan lactate having a number average molecular weight of less than 5,000. Functional chitosan lactate dietary supplement composed of less than. Chitosan swelling step of dispersing chitosan in the range of 60-90% deacetylation in water, dissolving the swollen chitosan in lactic acid solvent and enzymatically digesting with chitosanase, separating the decomposed chitosan lactate by molecular weight and drying And, mixing chitosan lactate separated by molecular weight in a predetermined ratio.