KR20020080238A - Extract from a carp having the effect of a medicine - Google Patents

Abstract

PURPOSE: To provide a carp extract having medical effect excellent in safety. CONSTITUTION: This carp extract is prepared by subjecting the carp to solvent extraction under a heating condition. The extract has blood glucose level increase-preventive effect, gastric mucosa damage-preventive effect, depression- treating effect, neurosis-treating effect, immune strength-enhancing effect, etc.

Description

Extract from carp having medicinal effect {Extract from a carp having the effect of a medicine}

The present invention relates to an extract of carp having a medicinal effect and a composition containing the same.

In recent years, medicines derived from natural organisms (including so-called herbal medicines and herbal medicines) have attracted more attention than medicines chemically synthesized in fields such as medicine. This is because synthetic drugs have the advantage that their efficacy is very excellent, while there is a risk of causing some side effects depending on the dose. As such, when side effects occur, administration of a medicament for further suppressing the side effects is required, which may cause further problems.

On the other hand, substances isolated from living organisms in nature, especially those eaten by humans from whales, are considered to be excellent in safety in experience. In addition, it can be inferred that organisms are diverse in response to their living environment and the like, and substances separated from them have unknown potential as medicines.

From whales, carp is widely eaten as a protein source that is not only excellent in taste but also healthy and valuable protein source. Against this background, carp is expected not only to be a protein source, but also to have some efficacy, and if this can lead to the development of medicines for various diseases that are currently important issues, it also leads to social contribution.

Accordingly, it is an object of the present invention to provide an extract of carp having excellent safety and a composition containing the same.

1 is a chart showing the IR spectrum of carp oil in one embodiment of the present invention.

2 is a chart showing an IR spectrum of a carp extract in another embodiment of the present invention.

MEANS TO SOLVE THE PROBLEM In order to achieve the said objective, the present inventors piled up earnest research regarding the efficacy of carp, and were able to obtain the extract of the carp which has the outstanding drug effect as shown below.

Carp extract of the present invention is an extract having a medicinal effect, and is an extract of carp used as an anti-hyperglycemic agent, gastric mucosa damage inhibitor, antidepressant, neurosis treatment, immune enhancer. The gastric mucosa impairment inhibitor is particularly preferably used as an alcohol-induced gastric mucosa impairment inhibitor, and the immunity enhancing agent preferably acts by having a macro purge activating action. According to such carp extract having various medicinal effects, since the safety is high, it can be used, for example, as a therapeutic drug which does not cause side effects.

The carp extract of the present invention is preferably an extract obtained by solvent extraction of carp under heating conditions, and more preferably, an oily fraction fraction separated after the heat treatment (hereinafter, the fraction is referred to as "carp oil"). And at least one of the aqueous phase fraction (hereinafter referred to as "carp extract"). The carp extract may be either carp oil or carp extract, or may contain both.

In the present invention, when the extract of carp is used as the blood glucose level increase inhibitor, the antidepressant drug and the neuropathy agent, it is preferable that the extract of the carp is carp oil because of its excellent effect. Moreover, when used as said gastric mucosal damage inhibitor, carp oil and carp extract are preferable, and carp extract is more preferable because it is excellent in drug efficacy. When used as said immunity enhancer, carp extract is preferable because of its excellent drug efficacy.

In the present invention, the carp oil is myristic acid, palmitic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linoleic acid, arachidonic acid, 5, 8, 11, 14, 17-eicosapentaenoic acid and 4, 7 , 10, 13, 16, 19-docosahexaenoic acid.

In the present invention, the solvent is preferably a solution or water containing alcohol. Specifically, for example, water, pure rice sake, sake, distilled liquor such as shochu or frothy bubbles can be used, preferably pure rice sake, water, and particularly preferably pure rice. Cheongju. In addition, as the alcohol, for example, ethanol, methanol, propyl alcohol and the like can be used, preferably ethanol, methanol, particularly preferably ethanol.

When the extract of the carp of the present invention is the carp oil, the solvent is preferably pure rice wine, sake, water, shochu, foam sake, and more preferably pure rice wine. In addition, when the extract of a carp of the present invention is the carp extract, the solvent is preferably water and pure rice wine, more preferably pure rice wine.

In the present invention, the solvent preferably further contains sucrose, fructose, glucose, salt, and the like for the purpose of antiseptic and salting. In particular, when the extract of carp of the present invention is carp oil, it is preferable to salt using a solvent containing such a substance.

In this invention, it is preferable that the ratio of the said solvent with respect to a carp is 1.5 to 5 times weight range, More preferably, it is the range of 2 to 5 times weight, Especially preferably, it is the range of 2.5 to 4 times weight.

In the present invention, the extract of the carp is not particularly limited, and examples thereof include extracts of whole carp, extracts of shells, scales, intestines and bodies. In addition, the scale part which is normally discarded has a function of promoting nerve transfer, such as the inside of the antler (mainly keratinous part), and the shell part has a hormonal action property, for example, the same part of the antler. It is conceivable, and it is preferable to extract these without discarding them because they can be effectively used at low cost.

In the present invention, the form of the extract of the carp is not particularly limited, and may be, for example, powdery or liquid (including paste).

Next, the composition of the present invention contains the carp extract of the present invention. If this composition contains the carp extract of the said invention as a main component or an active ingredient, it will not restrict | limit especially in other components, According to the use, it determines suitably. For example, when the composition is a pharmaceutical, the formulation is the carp extract as a main ingredient or an active ingredient, and is a solid or liquid excipient, and in the case of an internal medicine, powdered medicine, tablets, capsules, teas, granules, liquids (Like spirit, tincture, rheumatic extract, syrup, etc.) There are also types of injections, ointments, solutions, poultices, herbals, sprays, nourishing tonics, emulsions and the like. As the excipient, those known in the art may be used. For example, excipients for internal powders such as powdered medicines, granules, capsules, tablets, lactose, starch, dextrin, calcium phosphate, calcium carbonate, synthetic or natural aluminum silicate, magnesium oxide, dry aluminum hydroxide, magnesium stearate, sodium bicarbonate, Dry yeast, and the like. Examples of the excipient of the powder for external application include zinc oxide, talc, starch, khakirin, boric acid powder, zinc stearate, magnesium stearate, bismuth bismuth, potassium aluminum sulfate powder and the like. Examples of the excipient in the liquid preparation include water, glycerin, propylene glycol, syrup, ethanol, fatty acid, ethylene glycol, polyethylene glycol, sorbitol, and the like.

In addition, in the case of using the composition of the present invention as a health food or food additive, it can be used in the form of tablets, capsules, granules, powders, liquids, etc., but in a form that can be clearly distinguished from pharmaceuticals desirable. In addition, the above-mentioned thing can be used as an excipient in this case.

In the composition of the present invention, the content ratio of the carp extract can be appropriately determined depending on the use, form, number of doses, and symptoms of the patient.

[Embodiment of the Invention]

The carp oil of the present invention can be produced, for example, as follows.

For example, a whole carp is put in one solvent and heat-treated and extracted. In order to improve the extraction efficiency, it is preferable to use the carp, which is made of chips and minch (chopped chips), for example.

As said solvent, the solution containing water, such as pure rice wine, water, etc. can be used as mentioned above. In the case of a solvent containing an alcohol, the alcohol concentration is, for example, in the range of 5 to 40%, preferably in the range of 10 to 20%, and more preferably in the range of 12 to 18%.

As mentioned above, the addition ratio of the solvent to the carp is preferably in the range of 1.5 to 5 times by weight, more preferably in the range of 2 to 5 times by weight, and particularly preferably in the range of 3 to 4.5 times by weight. Specifically, when the solvent is pure blue wine, the range of 2 to 5 times the weight is preferable, More preferably, it is the range of 3 to 5 times weight, Especially preferably, it is the range of 3.5 to 4.5 times weight.

The solvent may be added with a substance such as sugars such as sucrose, glucose and fructose, salts such as salt, and the like for the purpose of removing or salting preservatives, proteins and the like as described above. Fructose, more preferably sucrose. The addition ratio of these substances is, for example, in the range of 100 to 200 g, preferably in the range of 120 to 180 g, and more preferably in the range of 140 to 160 g with respect to 1 L of the solvent. Specifically, in the case of using sucrose, it is, for example, in the range of 100 to 200 g, preferably in the range of 120 to 180 g, and more preferably in the range of 140 to 160 g with respect to 1 L of the solvent.

The time required for the extraction treatment by heating is, for example, in the range of 8 to 50 hours, preferably in the range of 24 to 50 hours, more preferably in the range of 40 to 48 hours, and the solvent temperature upon heating is, for example, It is the range of 70-100 degreeC, Preferably it is the range of 75-90 degreeC, More preferably, it is the range of 80-85 degreeC.

After the heat treatment, the carp is removed from the solvent to recover only the remaining solvent. This is an extract of carp. When this solvent is separated into an aqueous phase and an oil phase and the oil phase is recovered, carp oil can be obtained. The separation method of the oil phase is not particularly limited, but methods such as stationary separation and centrifugation may be employed. The yield of carp oil obtained by this method is usually in the range of 1.5 to 5% with respect to the weight of the carp, preferably in the range of 2 to 4%, and more preferably in the range of 2.5 to 3.5%. The carp oil of the present invention may be used as it is without being separated as described above. The separated aqueous phase can be used as a carp extract.

In addition, the obtained oil phase (carp oil) may be removed, for example, by washing with water. The water washing may, for example, add water to the oil phase, mix and stir the oil phase, and then fractionate the oil phase again. The amount of water added is, for example, in the range of 0.5 to 3 L, preferably in the range of 0.7 to 2.5 L, and more preferably in the range of 0.9 to 1.1 L with respect to 1 L of oil phase. In addition, the frequency | count of water washing is the range of 1-3 times, for example.

On the other hand, the extract of the carp of this invention can be manufactured as shown below, for example.

In the same manner as in the production of the carp oil of the present invention, one whole carp is solvent-extracted by heating. Although the same can be used as said solvent, For example, it is preferable to use the alcohol solution of 5 to 40% of alcohol concentration, Preferably it is 10 to 20%, More preferably, it is 12 to 18%. Specifically, pure rice wine is preferable.

In addition, the carp may be immersed in the solvent, for example, for 1 to 2 days in order to increase the penetration of the solvent into the tissue of the carp before the heat treatment.

Then, after the same heat treatment as the preparation of the carp oil, the carp is removed from the solvent to recover only the solvent. This is an extract of carp. The solvent is separated from the aqueous phase and the oil phase, and the oil phase is removed to recover the aqueous fraction. The carp extract can be obtained. The separation method of the aqueous phase fraction is not particularly limited and can be carried out as described above. The removed oily fraction can be used as carp oil.

Since carp extract is an aqueous phase fraction, it can also be used, for example, to concentrate, to make it powdery by lyophilization, etc.

Although the kind of carp used for this invention is not restrict | limited, For example, common carp, river carp (cultured carp), such as blue-colored carp and black carp, fish carp (鏡 鯉); German carp, such as a scale carp, etc. can be used. Among these, when used for the carp oil of this invention, since the oil component is many, the blue color carp is especially preferable.

(Example)

Next, examples of the present invention will be described. In addition, the carp provided in the following Example is a blue carp (blue carp).

(Example 1)

This embodiment is an example confirming the properties of carp oil and carp extract.

(Preparation method of carp oil and carp extract 1)

The whole carp of about 1.5 kg was made into pieces, 3.6L pure rice wine was added as a solvent, and it was stewed for 48 hours by the low heat so that the temperature of the said solvent might be about 80-85 degreeC. Then, the boiled carp was placed in 1.8 L of pure rice wine, cooled to room temperature, and then pressed under a pressure of 5.5 to 6.5 kg / cm 2. The compressed juice was concentrated to 80 mL at 80 to 85 ° C. to remove the alcohol content, and left overnight to separate the aqueous phase and the oil phase. Then, the aqueous phase was filtered with a water-containing cotton cloth filter in which water-containing cotton cloth was used as a filter cloth to obtain 900 g of carp extract (yield 60%). This was used as a sample of carp extract. In addition, the separated oil phase was washed with the same amount of water, and second, stationary separation gave 15 g (yield 3%) of an oil phase (carp oil). This was used as a sample of carp oil.

(Preparation method of carp oil Ⅱ)

The whole 1.5 kg of carp was cut into pieces, and 4 L of sucrose solution (concentration 15 weight%) was added to this as a solvent, and it was left to heat overnight after reflux cooling for about 8 hours (solvent temperature: about 80-85 degreeC). . The carp was removed from the sucrose solution, and the remaining solution was allowed to stand overnight to separate the aqueous phase and the oil phase. This oil phase (upper layer) was collected and washed twice with the same amount of water to obtain 15 g of carp oil (3% yield). This was used as a sample.

The properties of the carp oil obtained as described above are shown below.

(Appearance and Appearance of Sample)

Oily liquid with pale yellowish brown color with a characteristic odor. Insoluble in water, insoluble in ethanol, soluble in ether, benzol, chloroform, carbon tetrachloride.

(Physical appearance of the sample)

Oxidation value 1.7 (mg)

Saponification value 191.8 (mg)

Iodine

Fire Soap 1.7

(Gas chromatography)

The samples were saponified according to the Standard Maintenance Analysis Method (2 · 4 · 10-71), and the fluorinated saponified products were used as saponified fatty acids, and on the other hand, the Standard Maintenance Analysis Method (2 · 4 · 20-711) was used. Methyl esterification was carried out accordingly. Then, these treated samples were analyzed under the following gas chromatography conditions, and the compositional content (%) of fatty acids was examined. These results are shown in Table 1 below. In addition, the content rate was calculated | required from the peak area of the obtained chromatogram.

(Analysis condition)

Column: 2m × 3mm

20% DEGS on Chromosol Part W AW-DMCS

(60-80 mesh)

Temperature: Inlet 250 degrees Celsius

Column 210 ℃

Nitrogen gas flow rate: 35mL / minute

Detector: Flame Ionization Detector (FID)

Table 1

Holding time (minutes) Substance name Contents (%) 2 minutes 08 seconds Myristic acid 1.063 minutes 22 seconds Palmitate 18.483 minutes 52 seconds Palmitranic acid 5.755 minutes 30 seconds Stearic acid 3.826 minutes 17 seconds Oleic acid 39.397 minutes 34 seconds Linoleic acid 11.269 minutes 44 seconds Linoleic acid 18.3116 minutes 04 seconds Arachidonic acid 0.6420 minutes 56 Sec 5, 8, 11, 14. 17- eicosac ventanoic acid 0.4839 min 55 sec 4, 7, 10, 13, 16, 19- docosa hexaenoic acid 0.79

(UV absorption spectrum: UV)

(1) Measuring equipment: Shimadzu UV-1200 spectrophotometer

(Brand name, product made by Shimadzu Corporation)

(2) Measurement result: UV (CHCl ₃ , λ max, ㎚): 277.2, 236.6

(Infrared absorption spectrum: IR)

(1) Measuring equipment: Shimadzu FT-IR-8100 type device

(Brand name, product made by Shimadzu Corporation)

(2) Measurement result (Nujol, unit cm ^-4 ):

It has characteristic absorption in 3009 (methyl group, methylene group), 1748 (carbonyl group), 1653 (unsaturated condensation), 1464 (methyl group, methylene group), 1168, 1099 (br, hydroxyl group, unsaturated acid ester), 723 (unsaturated condensation) . This result is shown by the chart of FIG.

(Nuclear Magnetic Resonance Spectrum: NMR)

(1) Measuring equipment: Nippon Denshi EX-270 NMR machine (manufactured by Nippon Denshi, ie JEOL LTD.)

(2) Measurement result

¹ H-NMR (CDCl ₃ , 270 MHz, δ): 03.87 (2H, t-like), 1.25 (10H, br), 1.61 (1H, m), 2.03 (2H, m), 2.30 (1H, t- like), 2.80 (1H, m), 4.21 (1H, ABq), 5.33 (1H, m).

The properties of the carp extract obtained as described above are shown below.

(Growth of samples)

Oily liquid with a viscous reddish brown color with a characteristic amino acid odor. Soluble in water, turbid and soluble in methanol, ethanol, poorly soluble in butanol, insoluble in ether, ethyl acetate, benzene, chloroform.

(Color reaction of sample: detection of protein and amino acid)

10 mL of ethanol is added to 0.1 g of the sample, and it is heated in a water bath, and this is filtered. 0.5 mL of sodium hydroxide solution is added to the filtrate, and 3 to 4 drops of copper sulfate solution are added. The mixture is blue-purple.

(Thin Layer Chromatography of Sample)

5 mL of chloroform-methanol mixed solution (2: 1) was added to 0.1 g of the sample, and the mixture was slowly filtered for 30 minutes. The filtrate was then tested by the Pharmacopoeia General Test Method No. 29 thin layer chromatography method under the following conditions. As a result, a reddish purple spot having an Rf value of about 0.3 was identified.

(Condition)

Filtrate sample volume: 10μL

Carrier: Kieselgel 60F ₂₅₄ 0.25mm thick (Melk)

Developing solvent: Chloroform: Methanol: Mixture of water (65: 35: 10)

Deployment distance: 10㎝

Detection: 99 g of 10% sulfuric acid and 1 g of cerium sulfate (Express: Wako Pure Chemical Industries, Ltd.)

Spray 1% dicerium sulfate-10% sulfuric acid solution prepared by adding and dissolving

Then heated to 105 ° C. for 5 minutes

(Infrared absorption spectrum: IR)

(1) Measuring equipment: Shimadzu FT-IR-8100 type device

(Brand name, product made by Shimadzu Corporation)

(2) Measurement result (KBr, unit cm ^-1 )

It has characteristic absorption in 3346 (hydroxyl group), 2932 (methyl group, methylene group, methine group), 1864 (unsaturated condensation), 1452 (br, methyl group, methylene group, methine group), and 1041 (hydroxyl group, ether bond). This result is shown by the chart of FIG.

(Nuclear Magnetic Resonance Spectrum: NMR)

(1) Measuring equipment: Nippon Denshi EX-270NMR

(Brand name, product made by Nippon Denshi Corporation)

(2) Measurement result

¹ H-NMR (D ₂ O, 270 MHz, δ): 1.18 to lambda 1.23 (m), 1.37 (s), 1.39 (s), 3.37 (s), 3.5 to 3.9 (br) Have

(Example 2)

Confirmation of hyperglycemia

Carp oil prepared in Example 1 was orally administered (500 mg / kg) to Wistar male rats (about 130 g in weight) fasted for about 20 hours, and after 30 minutes, ordose (1.0 g / kg) was orally administered. Was administered. After 1 hour and 2 hours after that 30 minutes, about 0.2 ml of blood was collected from the orbital vein. The collected blood was centrifuged at 3000 rpm to obtain serum, and blood glucose levels were measured by the glucose oxidase method using a commercial kit (glucose CII test Wako, Wako Pure Chemical Industries, Ltd.). In addition, the blood glucose level of the rat without administration of carp oil and sucrose as a normal group was measured together, and the blood glucose level of the rat without administration of carp oil as a control was measured. The rats given for the experiment were 5 in each treatment group. These results are shown in Table 3 below. In the same table, the values are expressed as mean ± standard error (significant difference: ^** p <0.01).

Table 3

Treatment Group Dose Blood Glucose Level (mg / 100ml) mL / kg 0.5h 1.0h 2.0h Normal group-60.4 ± 9.1 84.7 ± 7.5 82.8 ± 4.4 Control group-163.0 ± 8.9 153.8 ± 11.2 117.6 ± 1.4 Carp oil 5.0 75.5 ± 7.9 ^** 106.1 ± 9.5 ^** 109.8 ± 4.6

As apparent from Table 3, the increase in blood glucose level was significantly suppressed by the administration of carp oil. From this, it was suggested that carp oil can prevent postprandial hyperglycemia and found it useful for preventing or improving diabetes.

(Example 3)

Identification of Ethanol-induced Acute Gastric Mucosal Injury Inhibitory Effect

After fasting SD male rats (about 250 g in weight) for about 24 hours, carp extract or carp oil prepared in Example 1 was orally administered (dose 250 mg / kg, 500 mg / kg). After 1 hour, 99.5% ethanol was orally administered at a rate of 1.5 ml / horse, and after 1 hour, ether was lethal and the stomach was extracted. The stomach was treated with 1.5% formalin to fix the tissue, and then cut in accordance with Taiwan, and the maximum diameter of the damaged part (damage coefficient (mm)) generated in the distal part was determined. In addition, the degree of damage was expressed as a score based on the criteria shown below by the number, length, and width of the damage. In addition, as a control, the damage coefficient (mm) and the score of the damage degree were similarly calculated | required about the rat which did not administer the said carp oil or carp extract. In addition, inhibition rate (%) was calculated | required by following formula (1). These results are shown in Table 4 below. In the same table, the values are expressed as mean value ± standard error (significance difference: P <0.01).

% Inhibition = 100-[(C / A) × 100]... (One)

A: Damage coefficient of rats administered ethanol extract

C: damage factor of the control rat

(Score)

0: no damage

1: Less than 5 total damage less than 5 mm in total length and less than 2 mm in width

2: 5 or less total length less than 5 mm in total length and less than 2 mm in width

3: Less than 5 intermediate damages of less than 5 mm in total length and less than 2 mm in width

4: More than 5 medium damages over 5mm in total length and 2mm in width or less

5: 1 to 3 hemorrhagic bands of moderate damage of less than 5 mm in total length and 2 mm or more in width

6: 4 or more hemorrhagic bands with moderate damage of less than 5 mm in total length and 2 mm in width or more

7: 1 to 3 hemorrhagic bands with severe damage of 5 mm or more in total length and 2 mm or more in width

8: 4 to 6 hemorrhagic bands with severe damage of more than 5 mm in total length and more than 2 mm in width

9: 7 or more hemorrhagic bands with severe damage of more than 5 mm in total length and more than 2 mm in width

Table 4

Sample Dose N Damage Factor Inhibition Rate Score Type (mg / kg) (mari) (mm) (%) Control-8 173.8 ± 17.0-7.8 ± 0.6 Carp Extract 250 6 44.2 ± 12.8 74.6 3.5 ± 2.0 Carp Extract 500 6 24.8 ± 11.6 85.7 2.4 ± 0.8 Carp Oil 250 6 108.4 ± 22.8 37.6 5.6 ± 0.6 Carp Oil 500 6 82.6 ± 8.4 52.4 4.4 ± 0.4

As is apparent from Table 4, carp extract and carp oil showed gastric mucosal damage inhibition, in particular carp extract showed significant inhibition.

(Example 4)

Confirmation of immunity enhancing action (macro fuzzy activity)

Animals have a macro purge (macrophage) that has a phagocytic effect of digesting and digesting foreign bodies such as bacteria into the cells as a function of immune defense to the body. Nitrogen monoxide (NO) produced from the macro purge involved in this action was measured to evaluate the macro purge activity of the carp extract.

(NO measurement of macro purge)

Macro purge was taken from the abdominal cavity of ddY male mice (approximately 30 g body weight), suspended in RPMI-1640 medium containing 10% fetal bovine serum (FCS), and 5 × 10 ⁵ cells in 96-flat basal microplates. / ml Seed and incubated for 1 hour (37 ° C, 5% CO ₂ ). Subsequently, the medium was exchanged with a medium containing various concentrations of the carp extract (concentration: 3, 10, 30, 100, 300 µg / ml), and incubated for 20 hours. Since NO is unstable and difficult to measure directly, NO ₂ , a commonly used metabolite of NO, was quantified using a Gries' reagent (Griess method). That is, the culture supernatant and the same amount of grease reagent (1% sulfonylamide / 0.1% N-1- naphthylethylenediamine / 5% phosphoric acid) are mixed, and allowed to stand at room temperature for 10 minutes, and then the absorbance is measured (measured and measured). Wavelength: 570 nm, reference wavelength: 655 nm), and quantified based on NaNO ₂ diluted in the medium. In addition, the above operation was used as a control except that the said carp extract was not added. The rats provided for the experiment were 4 in each treatment group. The results are shown in the table below. In addition, in the same table, the value was shown by the mean value + standard error (significance difference: P <0.01).

Table 5

Sample concentration NO product (μM) increase magnification Control-0.6 ± 0.2-Carp extract 3 3.0 ± 0.5 5.010 9.4 ± 0.3 15.630 10.8 ± 1.0 18.0 100 16.0 ± 0.5 26.6 300 15.8 ± 1.3 26.3

As apparent from Table 5 above, it was clear that the carp extract exhibited macro purge activating action because the production amount of nitrogen monoxide in the macro purge was increased by the addition of the carp extract. Accordingly, carp extract was found to be useful as an immune booster.

(Example 5)

Depression sickness patients and neuropathy patients, oral administration of the carp oil of the present invention prepared in Example 1 to investigate the improvement effect of depressive symptoms.

(Effective Example 5-1 of Treatment)

(1) Patient: Patient with depressive neurosis (38 years old male)

(2) Medical history: The constitution of the emotional ups and downs from birth, but deterioration of the economic condition of the home, the stress of daily life, mentally depressed state, worse work to stay in home did. I was diagnosed with depression in the psychiatry of the hospital, and I took oral medicine for the treatment of depression, and continued taking it for one month, but the condition did not improve, and I stayed in the house all day, and it became unstable to meet a person There was no desire at all.

(3) Treatment details and results: On an empty stomach, 5 g of carp oil was taken three times a day. As a result, feeling becomes refreshing by taking of two days, anxiety to meet with person disappears, motivation to work appears, and after one week improves to normal state, and can return to work completely, and is in a healthy state completely Recovered. After that, it does not recur and maintains a steady state.

(Effectiveness of Treatment Example 5-2)

(1) Patient: Severe depressive patients (78-year-old female)

(2) Medical history: It was nervous from birth, and depression state was repeated regularly for 30 years and continued taking mental stabilizer, but improvement of depressive condition was in trouble.

(3) Treatment details and results: 5g of carp oil was taken three times a day. As a result, by taking it for 1 year or more, it was very mentally stable, the mood was stabilized, and the depressive symptom was not expressed in the urine at all. At the time of a depressed state, this patient became a delusional state, but since taking carp oil, there is no delusion at all.

(Effectiveness of Treatment Example 5-3)

(1) Patient: Patient with neurosis (68 years old male)

(2) Medical history: nervousness, unstable mood, and the state of fuss continues, and even small things are angered and sleeplessness continues without using a large amount of mental stabilizers.

(3) Treatment details and results: Carp oil 15g was taken twice a day. As a result, about one month's use improved the mood and sensation of mood, the ongoing unstable mental state, and improved anger. Work is agriculture, but it is not tiring, and work is progressing well. In addition, the use of mental stabilizers is no longer necessary.

(Example 5-4)

(1) Patient: Patient with schizophrenia (40 year old woman)

(2) Medical history: As a schizophrenic, the administration of antipsychotic drugs continued for two and a half years, but the facial expressions were insignificant, the change in emotion was not seen, and the change in the expressionless face and the eyes was not recognized.

(3) Treatment details and results: Carp oil was taken 10g daily, in addition to the neuropathy medicine. As a result, after about two months of use, facial expressions appeared on the face, a smile, and a change in emotion appeared. Although cure for schizophrenia is considered difficult, improvement of hospital bed is clearly recognized by showing change of feeling in expression, eyes.

(Example 5-5)

(1) Patients: moderately depressed patients (male, 54 years old)

(2) Medical history: After being diagnosed with depressive illness, and continued administration of antipsychotic oral medicine for one year, helplessness became worse, future anxiety was not shaken, and depressive symptoms such as interpersonal fears worsened.

(3) Treatment details and results: Carp oil was taken 15 g per day in addition to the internal medicine. As a result, by taking about four months of administration, I became healthy every day, and the mental state became brighter, and the occurrence of depressive symptoms remained hardly. Subsequently, even if the internal medicine for psychosis was reduced, the symptoms were satisfactory, and the condition was recovered to the state which can be changed by administration of carp oil only.

(Example 5-6)

(1) Patients: Patients with depressive illness due to side effects of chronic joint rheumatoid medicine (70 years old female)

(2) History: For the treatment of 12 years of chronic joint rheumatism, the steroid hormone prednisolone (dosage 5 to 7.5 mg / day) was prevented for about 1 year and 6 months. Side effects, such as my moon fess, became recognized.

(3) Treatment contents and results: The dose of steroidoid hormone freednin was reduced for 4 months at 5 mg / day at intervals of 2 to 3 days, and the dose was reduced to 2.5 mg / day at 2 months. did. As a result, the deterioration of rheumatoid was not recognized, but symptoms and depressive symptoms such as steroid-specific angina due to a decrease in the dose of steroid hormones have been expressed and worsened. Here, even if the dose of pridnin was increased to 5 mg / day, no improvement in depressive symptoms was seen. Therefore, in addition to Friedin, carp oil 5 mg / day was administered. As a result, symptoms, such as angina, and depressive symptoms were slightly improved at two weeks of administration. Furthermore, after increasing the dose of carp oil to 10 g / day, after 10 days, symptoms such as angina and depressive symptoms disappeared and a clear improvement was possible. Moreover, by continuing the use of carp oil, the manifestation of depressive symptoms was not recognized at all.

By using the carp oil of the present invention as described above, it is possible to effectively alleviate the symptoms of depressive diseases and neurosis and to prevent their occurrence. In addition, since it is possible to suppress the depressive condition occurring as a side effect by administration of other therapeutic drugs, especially steroid hormones, it was understood that the side effects can be prevented safely and effectively even when used in combination with other therapeutic drugs.

As described above, the carp extract of the present invention exhibits efficacy against various symptoms such as suppressing blood sugar level increase, inhibiting gastric mucosal damage, depression, neurosis, and lowering immunity. Therefore, by using the extract of the carp of this invention, the said symptom can be improved safely and efficiently.

Claims (19)

An extract of carp, which is an extract of carp having a medicinal effect, and is used as an inhibitor of blood sugar level elevation. Extract of carp having medicinal effects, extract of carp, characterized in that used as a gastric mucosa damage inhibitor. The extract of carp according to claim 2, which is used as an inhibitor of alcohol-induced gastric mucosal damage. Extract of carp having medicinal effects, extract of carp characterized in that it is used as an antidepressant. An extract of carp having a medicinal effect, which is used as a therapeutic agent for neurosis. An extract of carp having medicinal effects, which is used as an immunity enhancer. The extract of carp according to claim 6, which has a macro purge activating action. The carp extract according to any one of claims 1 to 7, wherein the extract is an extract obtained by solvent extraction of the carp under heating conditions. The carp extract according to any one of claims 1 to 5, wherein the extract is an oily fraction which is separated after solvent extraction of the carp under heating conditions. The carp extract according to any one of claims 2, 3, 6 and 7, wherein the extract is an aqueous fraction separated from the carp after solvent extraction under heating conditions. The carp extract according to any one of claims 8 to 10, wherein the extract is an entire carp extract. The carp extract according to any one of claims 8 to 11, wherein the solvent is a solution or water containing an alcohol. The carp extract according to any one of claims 8 to 12, wherein the solvent further comprises at least one substance selected from the group consisting of sucrose, fructose, glucose and salt. The carp extract according to any one of claims 8 to 13, wherein the solvent is at least one solvent selected from the group of pure blue wine, shochu and highly bubbly. The carp extract according to any one of claims 12 to 14, wherein the alcohol is at least one alcohol selected from the group consisting of ethanol, methanol and propyl alcohol. The carp extract according to any one of claims 8 to 15, wherein the ratio of the solvent to the carp is in the range of 1.5 to 5 times by weight. The carp extract according to any one of claims 1 to 16, wherein the extract is at least one extract of scales and skins of carp. 18. The carp extract according to any one of claims 1 to 17, wherein the carp is a blue colored carp. The composition containing the extract of the carp of any one of Claims 1-18.