KR20020079478A - Inhibitor of lawn-disease containing hydrogen peroxide - Google Patents

Abstract

PURPOSE: A composition for inhibiting lawn grass disease containing hydrogen peroxide as an effective ingredient is provided which can effectively prevent and suppress lawn grass disease and remarkably improve the soil permeability while simultaneously remarkably reducing the amount of agricultural chemicals used. CONSTITUTION: The composition for inhibiting lawn grass disease contains 0.5 to 5.0% by weight of hydrogen peroxide, based on the total weight of the composition and additionally one or more general agricultural chemicals selected from the group consisting of tebuconazole, pencycuron, flutolanil, tolclofos-methyl and thiophanate-methyl. The lawn grass disease is a brown patch, yellow patch, Pythium blight, large patch, fairy-ring, etc..

Description

Grass disease inhibitor containing hydrogen peroxide as an active ingredient {INHIBITOR OF LAWN-DISEASE CONTAINING HYDROGEN PEROXIDE}

The present invention relates to a grass disease inhibitor comprising hydrogen peroxide as an active ingredient, and more particularly to kill mycelia and pests that cause grass disease and inhibit growth, thereby effectively preventing and inhibiting grass disease. At the same time it relates to a grass disease inhibitor comprising hydrogen peroxide as an active ingredient that can improve the growth of grass by improving the soil root permeability by improving the soil permeability.

The types of diseases can be broadly divided into infectious diseases caused by harmful organisms and non-communicable diseases caused by physical and chemical causes. Infectious diseases of plants are the most common fungal diseases caused by filamentous fungi, and other viral and bacterial diseases.

Golf course grasses are overwhelmingly caused by filamentous fungi (both fungi) in both Korean grasses and cool-season grasses. According to Couch, there are 64 species of grass disease in the world, and there are about 90 pathogens involved.

Among them, except bacterial bacterial and viral diseases (virus), the rest are all caused by fungus. There are about 20 kinds of important diseases that occur in golf courses in Korea, and the related pathogens are Rhizoctonia, Pythium, Sclerotinia, Agaricus, Typhula, Colletotrichum, Puccinia, Curvularia, Helminthosporium and the like.

As mentioned above, the number of types of disease caused by Korean golf courses is smaller than that of foreign countries because most golf courses are made of Korean grass, which is very resistant to grass disease.

On the other hand, about 60 kinds of various diseases occur in foreign golf courses centered on cold grass, because cold grass is very resistant to disease. Non-communicable diseases include biological factors such as algae and moss. Chemical factors include pesticides, fertilizers, animal waste, nutrient deficiencies, air pollution and oil spills. Temperature obstacles (high and low temperature), water and ice, dryness due to low soil depth, pressure, large accumulation and landscaping, and mechanical obstacles include mowing and scalping. have.

The main pests in Korean grass are large patches, fairy-rings and spring dead spots. The symptoms of large patches are most common in late autumn when grass enters dormancy and early spring when waking up from dormancy, but sometimes in the rainy season, in wet areas, and in the summer.

The size of the patch varies from about 60 cm in diameter to 6 m and is the largest among patch patches on the lawn.

The fall patch is circular and the edge of the lesion turns bright orange. Reddish brown to dark brown lesions are formed in the branch of the foliar infiltrated into the fermentation layer. The stem of infected grass is easily pulled out, and the branch of the leaf is squashed and dies, and a brown mycelium mat is formed on the surface of the stem. Lesions generally do not occur on the ground leaf tissue above the gluteal layer. Pathogens penetrate through the tube and destroy the branch, inhibiting the migration of nutrients and water, and eventually the leaves begin to turn bright orange. If the good environmental conditions persist, the condition becomes worse, killing more stems and turning the edge of the patch into orange, forming a larger patch.

Spring patches reappear in the same areas where autumn patches appeared. Lesions that occurred in the fall of the previous year are not germinated or very late in the spring, and increase in soil temperature and ground temperature in April and May, and when rainfall occurs during this time, the area is changed to light brown from the edge of the patch. Active patches continue throughout May and June, but are suppressed during the summer high temperatures (above 30 ° C) and the damaged areas (patches) gradually recover.

The pathogens of the large patch, Lizatonia solanie 2-2 belong to the root beetroot bacterial system, the optimum temperature for mycelial growth is 25-30 ℃, the onset temperature is 15-30 ℃, the optimum temperature is around 23 ℃. In addition, when the temperature reaches 10-15 ° C., the cell density rapidly increases and the grass starts to invade.

Large patch pathogens overwinter as mycelium or mycelia in diseased turf residue, flyfish, and live creeps. The fungus does not form spores and grows and spreads in the form of mycelium. The distribution density of the large patch pathogens in soil is the highest in the Daechi strata (within 3 cm of soil), and the infected area of grass is the branch stem of the Daechi strata.

The pathogenicity of large patch pathogens is high in Korean grasses (Z oysia japonica, Zoysia matrella, Zoysia tenuifolia ), while in the Korean paper grass species ( Agrotis palustris, Poa pratensis, Lolium prenne, Festuca rubra subsp. Rubra L. ).

Conventional control methods of the large patch include grass removal method, fertilization management method using calcined lime or siliceous fertilizer, toros hydrous (500 times, 1 L / ㎡), chemical control method using pencicuron hydrous, terbuconazole oil, etc., The spring blight disease uses a strong drug such as a penetrating agent as a prophylactic agent, and a large patch or a brown patch control agent is used as a control agent.

The large patch of Korean grass as described above is increasing as the density of Lirtonia solani mycelium increases due to the grass growing, so the chemical, biological and seedling methods are used to control the large patch. It is mobilizing.

Fairing also occurs mainly on wet lawns from spring to early summer. This is an excessive nitrogen component produced by the decomposition of organic matter in the soil by the microorganisms in a wet lawn, which causes a round ring-shaped green ring on the grass. The grass at the lesion part of the fairing ring has a strong growth demand and has a high moisture demand.If the fungal hyphae forms a mycelial mat in the root zone, the hydrophobicity of the hyphae prevents water from penetrating into the soil pores, thereby maximizing the water shortage phenomenon. Drying causes the grass to die. In addition, a lot of soil occurs in the grass of soft soil with low fertility and insufficient humidity.

On the other hand, the main diseases of cold grass are brown patch, yellow patch, pythium blight, anthracnose, dollar spot, tongue phalanx and typhula blight. ) Is representative.

Brown patches occur at temperatures above 20 ° C. and at least 75% humidity in June to September. The most common pathogen of brown patches is Lizatonia solani, and brown lesions of several centimeters in diameter occur at first, but they expand to about 1 m in 3 to 12 hours and form donuts. It becomes color and dies.

As a method for controlling grass disease, which is being studied recently, a method using microorganisms, Korean application No. 2000-3114 includes at least two or more fungi and Bacillus subtilis, including Trihoderma garzianum. It describes a biopesticide composition for controlling phytopathogens comprising at least two bacteria as the main active ingredient, Bacillus subtilis GB-0365 (Accession No .: KFCC-11071) in the Republic of Korea Application No. 1998-54040 ) And Bacillus genus GB-017 (Accession No .: KFCC-11070) are described as a method for providing a pest prevention material of crops.

However, the conventional methods are uneconomical because the reduction rate of pesticide use is insignificant, and there is a problem that not only the effect of inhibiting turf disease is insignificant, but also lowering the soil permeability to inhibit the growth of grass.

Therefore, a significant reduction in the amount of pesticides used can effectively prevent and suppress turf disease, and at the same time, research on turf disease inhibitors that can improve the soil permeability and improve the growth of turf is needed. It is true.

In order to solve the above problems, an object of the present invention is to provide a grass disease inhibitor that can effectively prevent grass disease by effectively inhibiting and killing the growth of mycelia or pests that cause grass disease.

It is another object of the present invention to provide a grass disease inhibitor that can not only significantly reduce the amount of pesticide used, but can also effectively prevent and suppress turf disease and significantly improve soil permeability.

Figure 1 shows the onset of the large patch when treated with hydrogen peroxide according to an embodiment of the present invention.

Figure 2 shows the inhibitory effect of Lizatonia solanii causing a large patch with or without the addition of hydrogen peroxide according to an embodiment of the present invention.

In order to achieve the above object, the present invention provides a grass disease inhibitor comprising hydrogen peroxide (H ₂ O ₂ , hydrogen peroxide) as an active ingredient.

Hereinafter, the present invention will be described in detail.

In order to control plant diseases, the present inventors sprayed hydrogen peroxide, which was used as a pesticide adjuvant by mixing extremely low concentrations with conventional pesticides, to Korean grass and cold grass, and suppressed the growth of mycelia and pests causing grass diseases. It was confirmed that there is an excellent effect on killing, to complete the present invention based on this.

The present invention relates to a grass disease inhibitor comprising hydrogen peroxide as an active ingredient, wherein the hydrogen peroxide prevents and suppresses grass disease by reducing the density of pathogens or pests in soil by killing mycelia or pests causing grass disease. It is characterized by.

The grass disease inhibitor of the present invention is preferably applied to a strain or pests that cause turfgrass in Korean grass or cold grass, and more preferably, brown patch or yellow patch generated by mycelia. , Turfgrass such as pythium blight, grass turf of Korean grass such as large patch, fairy-ring, and pests such as moths, scarabs, slugs and earthworms. It is good to apply it to the grass bottle.

In addition, the grass disease inhibitor of the present invention can not only inhibit and kill the growth of grass disease-causing strains or pests, but also improve the growth of grass roots by increasing the soil permeability, thereby improving the growth of grass.

Hydrogen peroxide used in the present invention is preferably included in 0.5 to 5.0% by weight, more preferably 0.5 to 4.0% by weight relative to the total weight of the grass disease inhibitor. When the content is less than 0.5% by weight, the prevention and suppression effect of turfgrass is insignificant, and when it exceeds 5.0% by weight, the economic efficiency is lowered.

Including 2.5 to 3.5% by weight of hydrogen peroxide relative to the total weight of the grass disease inhibitor can improve the mortality rate of pests that cause grass disease to 60% or more, in particular, the kill rate of slugs, slugs, to 70% or more. Can be improved.

In addition, the soil permeability can be improved to 30% or more when including 2.5 to 3.5% by weight of hydrogen peroxide relative to the total weight of the grass disease inhibitor of the present invention.

In addition, the turfgrass disease inhibitor of the present invention may further include a general pesticide component in the hydrogen peroxide.

As the general pesticide component, it may be used such as horicur (tebuconazole), montserene (pencycuron), montkat (flutolanil), lyclox (methyl), or geopanelating agent. The general pesticide is preferably diluted or used as a stock solution, more preferably using a diluted solution diluted to 25%, or 50%.

The turfgrass bottle inhibitor of the present invention may be prepared and used in any formulation, such as liquid, gel, powder, solid, pellets, preferably powder or pellets prepared by mixing the powder in a conventional manner.

In addition, the grass disease inhibitor of the present invention can be prepared in the form of fertilizers or soil improver. The fertilizer-type turf disease inhibitor can be prepared from siliceous (Si) fertilizer or calcareous (CaO) fertilizer, and can also be prepared as a complex fertilizer containing nitrogen, phosphoric acid, trace elements and the like.

In the present invention, for the prevention and suppression of turf disease, based on the amount of hydrogen peroxide contained in the turfgrass inhibitor, it is preferable to spray 1 to 3 g per m 2 of turf area, and the season is spring to autumn This is preferred. In the spray form of the turf disease inhibitor of the present invention, it is preferable to spray powder or pellets on the surface layer of grass.

Lawn disease inhibitor of the present invention is a grass disease caused by the mycelium grass patch of cold grass such as brown patch (yellow patch), yellow patch (yellow patch), pythium blight, and large patch (large patch) It is effective in the prevention and suppression of turfgrass in Korean grass such as, fairy-ring. Especially, it is very effective in the prevention and suppression of large patch in Korean turf caused by Littoonia solani mycelia. .

In addition, the grass disease inhibitor has an excellent effect on the prevention and suppression of turf disease caused by pests such as gangbang moth, scarab slugs, earthworms of golf courses, slugs by spraying scarab slugs on the grass bottle as a pathogen Has an excellent effect of killing more than 70%.

Hereinafter, preferred examples are provided to help understanding of the present invention, but the following examples are merely to illustrate the present invention, and the scope of the present invention is not limited to the following examples.

EXAMPLE

Example 1 Large Patch Control Effect in Korean Grass

Rhizoctonia solani 2-2 ( Rhizoctonia solani 2-2), a large patch pathogen, was inoculated into Korean grass and treated with sterile water, 0.5% by weight, and 0.8% by weight of hydrogen peroxide, respectively, to examine the development of large patches. It investigated and the result is shown in FIG.

Figure 1 shows the onset of the large patch when inoculated in grass turf inhibitors containing hydrogen peroxide (0.5% by weight, 0.8% by weight) in accordance with the present invention. A is a state in which normal sterile water is treated, B is 0.5% by weight of hydrogen peroxide, and C is 0.8% by weight of hydrogen peroxide. The incidence of large patches was large incidence within 10 days in the control group (A) treated with normal sterilized water, but in the treatment group treated with 0.5% by weight and 0.8% by weight of hydrogen peroxide after 30 days and 50 days, respectively. Developed. Through the above results, it can be seen that the treatment of hydrogen peroxide can delay the inhibition of the onset of Lizatonia solani hyphae causing a large patch for more than one month.

Example 2. Large patch suppression test (indoor test)

Soaking each Lixtonia solani hyphae causing a large patch in normal sterile water and 0.5% by weight of hydrogen peroxide in the room (soaking) to confirm the inhibitory effect of the large patch pathogens, the results are shown in FIG.

Figure 2 shows the inhibitory effect of Lizatonia solani hyphae causing a large patch with or without the addition of a grass disease inhibitor containing hydrogen peroxide of the present invention. In the control group soaking the large patch pathogen in normal sterile water, the mycelia of the large patch pathogen were not killed, but in the B which soaked the large patch pathogen in 0.5% by weight of hydrogen peroxide, the mycelia of the large patch pathogen were soaked for 1 hour. Was confirmed to be killed.

Example 3. Large patch suppression test (outdoor test)

In the soil, the litzatonia solani had a density of 532 propagules / 1 g, and 1 L of normal sterilized water and 0.5 wt% hydrogen peroxide were treated on the large patch of grass to confirm the inhibitory effect of the large patch pathogen.

As a result, the density of lytononia solanium in soil was reduced to 220 propagules / 1 g in the soil treated with 0.5% by weight of hydrogen peroxide after 3 days of treatment. There was no change in density. 7 days processing In the soil treated with 0.5% by weight of hydrogen peroxide, the liagonia solani's propagules were reduced by more than 50%, from which the hydrogen peroxide inhibited the large patch.

Example 4 Brown Patch Control Effect in Korean Grass

Rhizoctonia solani 2-1, a brown patch pathogen, was inoculated and treated with normal sterile water, 0.5% by weight, and 0.8% by weight of hydrogen peroxide, respectively.

As a result, brown patches developed within 8 days in the control group treated with normal sterilized water, but brown patches occurred after 18 days and 26 days in the treated groups treated with 0.5% by weight and 0.8% by weight of hydrogen peroxide, respectively. Through the above results, it can be seen that the treatment of hydrogen peroxide can delay the suppression of the onset of Lizatonia solanium causing brown patches.

Example 5. Brown patch inhibition test (indoor test)

The inhibitory effect of brown patch pathogens was confirmed by soaking Lihizoctonia solani 2-1, which causes brown patches in normal sterile water and 0.5% by weight of hydrogen peroxide, indoors.

Experimental results showed that brown patch pathogen mycelium was not killed in the control group soaked with brown patch pathogen in normal sterile water, but brown patch pathogen mycelium when soaked for more than 24 hours in the brown rat pathogen treated with 0.5 wt% hydrogen peroxide. Was confirmed to be killed.

Example 6. Brown patch suppression test (outdoor test)

Inhibitory effect of brown patch pathogens by treating with 1 L each of normal sterilized water and 0.5% by weight of hydrogen peroxide on the grass where brown patch is infested with 532 propagules / 1 g of Rhizoctonia solani 2-1 in soil It was confirmed.

As a result, after 3 days of treatment, the concentration of Lithonia solani 2-1 in soil was decreased to 321 propagules / 1 g in the treatment group treated with 0.5% by weight of hydrogen peroxide, whereas in the control group treated with normal sterile water, lizatoninia in soil was treated. There was no density change of the Solani 2-1. 7 days processing In the soil treated with 0.5% by weight of hydrogen peroxide in the soil treated with Littoonia solani 2-1 was reduced more than 30%, from which the hydrogen peroxide inhibited the brown patch.

Example 7 Yellow Patch Control Effect in Korean Grass

Yellow patch pathogen, Rhizoctonia cerealis , was inoculated and treated with normal sterile water, 0.5% by weight, and 0.8% by weight of hydrogen peroxide, respectively, to investigate the development of yellow patches.

As a result, yellow patches occurred within 10 days in the control group treated with normal sterilized water, but yellow patches occurred after 18 days and 30 days in the treated groups treated with 0.5% by weight and 0.8% by weight of hydrogen peroxide, respectively. Through the above results, it can be seen that treatment with hydrogen peroxide can delay the suppression of the onset of Lizatonia serearis causing yellow patches.

Example 8. Yellow patch suppression test (indoor test)

Inhibiting the effect of yellow patch pathogens was confirmed by soaking Lizatonia cerearis, which cause yellow patches in normal sterilized water and 0.5% by weight of hydrogen peroxide, indoors.

Experimental results showed that the yellow patch pathogen mycelium was not killed in the control group that soaked the yellow patch pathogen in normal sterile water, but the yellow patch pathogen was soaked when soaked for more than 12 hours in the treatment group that soaked the yellow patch pathogen in 0.5 wt% hydrogen peroxide. Was confirmed to be killed.

Example 9. Yellow patch suppression test (outdoor test)

In the soil, the concentration of Lizatonia serearis was 532 propagules / 1 g, and the yellow patches were spread on the lawn, and 1 L each of normal sterilized water and 0.5% by weight of hydrogen peroxide was confirmed to inhibit the yellow patch pathogens.

As a result, after 3 days of treatment, the density of Lithuanian cerearis in soil was decreased to 198 propagules / 1 g in the treatment group treated with 0.5% by weight of hydrogen peroxide, whereas in the control group treated with normal sterile water, There was no change in density. 7 days processing In the soil treated with 0.5% by weight of hydrogen peroxide, the total amount of Li footonia cerearis in the soil was reduced by more than 54%, and it was confirmed that hydrogen peroxide inhibited the yellow patch.

Example 10. Pisium blight control effect in Korean grass

The incidence of pisium blight was investigated by inoculating Pythium gaphanidermatum , which is a pisium blight pathogen, and treated with normal sterilized water, 0.5% by weight, and 0.8% by weight of hydrogen peroxide.

As a result, in the control group treated with normal sterile water, pisium blight developed within 7 days, but in the treatment group treated with 0.5% and 0.8% by weight of hydrogen peroxide, pisium blight occurred after 19 days and 26 days, respectively. Through the above results, it can be seen that the treatment of hydrogen peroxide can delay the suppression of the occurrence of piiumum apanidermathium which causes pisium blight.

Example 11. Pisium blight inhibition test (indoor test)

The inhibitory effect of pisium blight pathogens was confirmed by soaking pisium aphnidermium, which causes pisium blight in normal sterile water and 0.5% by weight of hydrogen peroxide, indoors.

As a result, the control group which soaked pisium blight pathogen in normal sterile water did not kill the mycelia of pisium blight pathogen, but in the treatment in which pisium blight pathogens were soaked in 0.5% by weight of hydrogen peroxide, it was pissible when soaked for 15 hours or more. It could be confirmed that the mycelia of the light pathogen were killed.

Example 12. Pisium blight inhibition test (outdoor test)

In soil, the density of pisium apanidermium was 545 propagules / 1 g, and 1 L of normal sterilized water and 0.5% by weight of hydrogen peroxide were treated on the grasses with pisium blight to confirm the inhibitory effect of pisium blight pathogen. .

As a result, three days after the treatment, 0.5% by weight of hydrogen peroxide decreased the density of pisium aparnidermium to 312 propagules / 1 g in the soil, whereas the control group treated with normal sterile water suffered pisium in the soil. There was no change in density of niermathium. 7 days processing In the soil treated with 0.5% by weight of hydrogen peroxide, the total weight of pisium apanidermathium in the soil was reduced by more than 40%. From this, it was confirmed that hydrogen peroxide inhibited piiumum blight.

Example 13 Effect of Fairing on Korean Grass

Inoculated with Marasmius in Scleroderma spp, Lycoperdon, and Marasmius spp., Including the genus Agaricus sp. The incidence of fairing was investigated by treatment with water, 0.5% by weight and 0.8% by weight of hydrogen peroxide, respectively.

As a result of the experiment, the incidence of the fairing ring was found to occur within 30 days in the control group treated with normal sterile water, but the fairing ring did not occur in the treatment group treated with 0.5 wt% and 0.8 wt% hydrogen peroxide, respectively. Through the above results, it can be seen that treatment with hydrogen peroxide can delay the suppression of the occurrence of marassius causing fairing.

Example 14 Fairing Suppression Test (Indoor Test)

The inhibitory effect of the pairing pathogen was confirmed by soaking Marasmius, which causes pairing in general sterilized water and 0.5% by weight of hydrogen peroxide indoors.

The experimental results showed that the mycelia of the fairing pathogens were not killed in the control soaked with fairing pathogens in normal sterile water, but the mycelia of the fairing pathogens were soaked for 10 hours or more in the treatments in which the fairing pathogens were soaked in 0.5% by weight of hydrogen peroxide. Was confirmed to be killed.

Example 15 Fairing Suppression Test (Outdoor Test)

In the soil with the density of Marasmius 320 propagules / 1 g in the soil, 1 L of normal sterile water and 0.5% by weight of hydrogen peroxide were treated on the lawns where the fairing was infested.

As a result, the density of marasmus decreased to 110 propagules / 1 g in the soil treated with 0.5% by weight of hydrogen peroxide after 3 days of treatment. There was no. 7 days processing After the 0.5 wt% hydrogen peroxide treatment in the soil treated with the total amount of Marasmius in the soil was reduced by more than 30%, from which it can be confirmed that hydrogen peroxide inhibits the fairing.

Experimental Example 16 Slugs Control Test

In the Anyang Benest test forge, slugs and hydrogen peroxide were mixed with soil at the same time, treated on the lawn, and the control effect was confirmed. Slugs, the grass-causing pests, were mixed in the soil and mixed with varying amounts of hydrogen peroxide at 0 wt%, 0.5 wt%, and 0.8 wt%, respectively.

Hydrogen peroxide was treated in Korean grass forge, and two weeks later, the density of grass pests, called slugs, was investigated. The treatment rate of slug was 50% or more in the treatment group treated with 0.5% by weight of hydrogen peroxide, and 90% or more in the treatment group treated with 0.8% by weight.

Experimental Example 17. Earthworm Control Test

Earthworm control test was measured by examining the number of earthworms protruding when the first spraying grass grass inhibitor containing hydrogen peroxide of the present invention as an active ingredient in a large patch, brown patch, fishium blight test.

division Number of protrusions (horses) 1 time Episode 2 3rd time Average No treatment 10 9 12 10.3 Hydrogen peroxide 0.5 wt% 3 3 2 2.7 Hydrogen peroxide 0.8 wt% One One One One

As shown in Table 1, according to the present invention was confirmed that the grass pest inhibitor containing 0.5% by weight, 0.8% by weight of hydrogen peroxide as an active ingredient exhibits a high control effect against earthworms compared to the untreated control. .

As described above, the turfgrass disease inhibitor of the present invention can not only significantly reduce the amount of pesticide used, but also can effectively prevent and suppress turfgrass disease by killing mycelia and pests causing grass disease and inhibiting growth. have. In addition, the turfgrass disease inhibitor of the present invention has the effect of improving the growth of the grass by improving the growth of grass roots by improving the soil permeability.

Claims (5)

Grass disease inhibitor containing hydrogen peroxide (H ₂ O ₂ , hydrogen peroxide) as an active ingredient. The method of claim 1, Lawn disease inhibitor containing the hydrogen peroxide is 0.5 to 5.0% by weight relative to the total weight of grass disease inhibitor. The method of claim 1, The grass disease inhibitor further comprises at least one general pesticide selected from the group consisting of horcucure (tebuconazole), montserene (pencycuron), montkat (flutolanil), risclox (tolclofos methyl), and a geopanelating agent Turf disease inhibitors. The method of claim 1, Lawn disease inhibitor is a formulation of the grass disease inhibitor is liquid, gel, powder, solid, or pellets. The method of claim 1, The grass bottle is a brown patch, yellow patch, yellow patch, pythium blight, large patch, fairy-ring, grass bottle caused by perishable moth, slugs Lawn disease inhibitors, which are turf diseases caused by earthworms or by earthworms.