CN111373903A - Method for removing fusarium wilt of bitter gourd seeds - Google Patents
Method for removing fusarium wilt of bitter gourd seeds Download PDFInfo
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- CN111373903A CN111373903A CN202010187348.1A CN202010187348A CN111373903A CN 111373903 A CN111373903 A CN 111373903A CN 202010187348 A CN202010187348 A CN 202010187348A CN 111373903 A CN111373903 A CN 111373903A
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- seeds
- bitter gourd
- fusarium wilt
- hydrogen peroxide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Abstract
The invention discloses a method for removing fusarium wilt of bitter gourd seeds. Experimental research shows that hydrogen peroxide can effectively sterilize bitter gourd seeds with fusarium wilt bacteria so as to reduce or remove the fusarium wilt bacteria carried on the bitter gourd seeds and cure disease seeds into healthy seeds. The method removes fusarium wilt bacteria from the seed source, has the advantage of ensuring the germination rate of seeds, is safe to the seeds, has good environmental compatibility, and has the advantages of simple operation and no need of special equipment; the hydrogen peroxide is low in price and easy to popularize in large scale in production, fills the blank of seed sterilization treatment for preventing and treating the bitter gourd fusarium wilt at present, effectively solves the problem of fusarium wilt of seed tapes, and has very important significance for solving the problem of serious diseases of the bitter gourd industry.
Description
Technical Field
The invention belongs to an agricultural seed treatment method, and particularly relates to a method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds.
Background
Bitter gourd Fusarium wilt is a typical soil-borne disease caused by Fusarium oxysporum bitter gourd specialization (sp. mordicae), and is also a seed-borne disease. Due to intensive production and continuous cropping for many years, the bitter gourd fusarium wilt quickly spreads in production areas, and becomes an important disease for restricting the development of the bitter gourd industry. At present, the bitter gourd fusarium wilt is mainly prevented and controlled by soil disinfection, grafting, biological prevention and control and the like, so that the possibility of infection of external fusarium wilt germs can be greatly reduced. However, these measures cannot effectively solve the problem that the seeds are self-infected, so that the occurrence of the wilt of bitter melons cannot be prevented.
The seed sterilization treatment is used as a key part in the field of crop protection, is mainly used for preventing and controlling spores or other structures generated by pathogens on the surfaces of seeds and killing pathogens infected in seeds, and has important significance for the health of the seeds and the normal growth and harvest of crops in the later period. The existing reports on seed germ control include: leather 3%, hymexazol, carbendazim etc. are thought to be effective in preventing and treating cucumber wilt in seedling stage; guodawei research shows that the 50% carbendazim wettable powder has a good effect on watermelon seed diseases; researches such as Zhang Qing Nu and the like show that the 0.01% acetic acid solution can greatly reduce canker carried by tomato seeds; the plum blossom plum considers that seeds treated by 25 percent of phenamacril SC and 24 percent of penflufen FS have good prevention and control effects on rice bakanae disease in the seedling stage; the Guolaying considers that 40% formalin 100 times liquid is used for soaking seeds for 20-30 minutes, and can prevent and treat tomato early blight, cucumber anthracnose, cucumber fusarium wilt and eggplant verticillium wilt. The prevention and treatment of the fusarium oxysporum f.sp.sp.cubense of the balsam pear seeds are not reported.
Hydrogen peroxide (H)2O2) Is a strong oxidant, and the water solution (commonly called hydrogen peroxide) of the strong oxidant is used for medical wound disinfection, environmental disinfection and food disinfection. In the agricultural field, hydrogen peroxide is mostly used for soaking seeds to improve the germination rate and the vitality of the seeds, for example, Yuanying discovers that hydrogen peroxide is used for soaking seeds for 16 hours in the 'influence of seed soaking time, germination accelerating temperature and medicament treatment on the germination of bitter gourd seeds', the germination rate and the germination potential are the highest, but hydrogen peroxide is not used for preventing and controlling bitter gourd blight bacteria.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the bitter gourd seed fusarium wilt pathogen removal method which is simple to operate, low in cost and easy to popularize.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for removing wilt germs of bitter gourd seeds comprises soaking bitter gourd seeds in hydrogen peroxide before germination.
The method for removing the fusarium wilt of the bitter gourd seeds is operated according to the following steps:
(1) soaking the bitter gourd seeds in hydrogen peroxide;
(2) cleaning the seeds after soaking treatment to remove residual hydrogen peroxide;
(3) and after cleaning, soaking the seeds for accelerating germination.
Hydrogen peroxide is H with the mass concentration of 5-20 percent2O2An aqueous solution of (a).
Hydrogen peroxide is H with the mass concentration of 10 percent2O2An aqueous solution of (a).
The soaking time is 5-20 min.
The soaking time is 10 min.
The cleaning is carried out for 4-5 times by adopting clean water.
Soaking the seeds in sterile water for 8-10h at the germination accelerating temperature of 30 ℃.
Soaking the seeds in sterile water for 10 h.
Aiming at the problems existing in the current prevention and treatment of bitter gourd fusarium wilt, the inventor establishes a method for removing the fusarium wilt of bitter gourd seeds, and hydrogen peroxide is adopted to soak the bitter gourd seeds before seed soaking and germination accelerating. Experimental research shows that hydrogen peroxide can effectively sterilize bitter gourd seeds with fusarium wilt bacteria so as to reduce or remove the fusarium wilt bacteria carried on the bitter gourd seeds and cure disease seeds into healthy seeds. The method removes fusarium wilt bacteria from the seed source, has the advantage of ensuring the germination rate of seeds, is safe to the seeds, has good environmental compatibility, and has the advantages of simple operation and no need of special equipment; the hydrogen peroxide is low in price and easy to popularize in large scale in production, fills the blank of seed sterilization treatment for preventing and treating the bitter gourd fusarium wilt at present, effectively solves the problem of fusarium wilt of seed tapes, and has very important significance for solving the problem of serious diseases of the bitter gourd industry.
Drawings
FIG. 1 is a graph showing the results of the inhibition of the bacteria of Momordica charantia seeds treated with different agents, in which: a Chunlei and copper oxychloride, b bleaching water, c hydrogen peroxide and d clear water.
FIG. 2 shows the results of the treatment of bitter gourd seeds with hydrogen peroxide of different concentrations.
Detailed Description
Example 1
Selecting 47% Chunlei King copper wettable powder 600 times liquid (Nippon Xinghuai chemical industry Co., Ltd.), bleaching water 10 times liquid (blue moon (China) Co., Ltd.), and 30% H2O2Plating 3 medicaments such as 10-time solution (Chongqing Chuandong chemical industry (group) Co., Ltd.), and using clear water as a control to research the bacteriostatic effect of different medicaments on the bitter gourd fusarium wilt.
Activating a fusarium oxysporum bitter gourd specialized strain CL10 ② -1;
preparing a sterilized PDA plate;
and (3) punching a bacterial colony edge bacterial cake by using a puncher with the diameter of 5mm, placing the bacterial cake at a position 1cm away from the center of a PDA (personal digital assistant) flat plate by using an inoculation needle, placing a soaked medicament filter paper strip at a position 1cm away from the center of the flat plate, and observing the bacteriostasis condition.
As shown in figure 1, research results show that the treatment of the chunlei copper oxychloride and the bleaching water has no inhibition effect on the fusarium oxysporum f.sp.cubense; in the hydrogen peroxide treatment, the blight bacteria close to one side of the medicament filter paper strip are inhibited from growing, which shows that the hydrogen peroxide is effective in inhibiting the balsam pear blight bacteria.
Example 2
Hydrogen peroxide solutions of different concentrations were prepared, different soaking times were set (see table 1 for details), study H2O2The treatment has effects on the effects of removing wilt germs of bitter gourd seeds and the germination rate of bitter gourd seeds.
TABLE 1 different H2O2Research on effect of treatment on removing fusarium wilt of bitter gourd seeds
Taking the balsam pear seeds with blight germina, treating 5 seeds per treatment, and repeating for 3 seeds. Treating the seeds in the medicament, washing with sterile water for 4 times, soaking in sterile water for 5min, draining, and placing in a sterilized PDA plate. And 3d, observing the removal effect of each treatment on the fusarium oxysporum f.sp.sp.cubense of the balsam pear seeds.
Taking the balsam pear seeds with blight germina, treating 30 seeds per treatment, and repeating for 3 seeds. The seeds are placed in a medicament for treatment, cleaned for 5 times by clear water, kneaded during cleaning, the clean cleaning of hydrogen peroxide on the surfaces of the seeds is guaranteed, the seeds are placed in the clear water for soaking for 10 hours, and then are laid flat and placed in a culture dish filled with filter paper, and a layer of filter paper is covered on the culture dish for moisturizing and accelerating germination at 30 ℃. And 5d, observing the germination condition of the bitter gourd seeds.
TABLE 2 different H2O2Treated bitter gourd seed germination
As shown in figure 2, the bitter gourd seed fusarium wilt can be well removed by 4 treatments, such as 10% hydrogen peroxide soaking for 10min (treatment 2-2), 10% hydrogen peroxide soaking for 15min (treatment 2-3), 15% hydrogen peroxide soaking for 10min (treatment 3-2), 15% hydrogen peroxide soaking for 15min (treatment 3-3) and the like. As shown in table 2, the germination rates of the 4 treated seeds were 86.67%, 80.00%, and 67.78%, respectively, which were reduced by 1.11%, 7.78%, and 20.00% respectively from the control germination rate of 87.78%.
The sterilization effect and the germination condition are combined, 10% hydrogen peroxide is soaked for 10min to be the best treatment, so that the effect of removing fusarium wilt germs of the balsam pear seeds can be ensured, and the germination quality of the seeds can also be ensured.
In conclusion, the short-time soaking treatment with hydrogen peroxide in the invention has a good effect of removing blight bacteria on balsam pear seeds, but has no obvious damage to the seeds and does not influence the normal germination of the balsam pear seeds. The method is obviously different from the report that the germination rate and activity of seeds can be improved by long-term soaking in hydrogen peroxide.
Claims (9)
1. A method for removing wilt germs of bitter gourd seeds is characterized in that the bitter gourd seeds are soaked by hydrogen peroxide before seed soaking and germination accelerating.
2. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 1, which is characterized by comprising the following steps:
(1) soaking the bitter gourd seeds in hydrogen peroxide;
(2) cleaning the seeds after soaking treatment to remove residual hydrogen peroxide;
(3) and after cleaning, soaking the seeds for accelerating germination.
3. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 2, wherein: the hydrogen peroxide is H with the mass concentration of 5-20%2O2An aqueous solution of (a).
4. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 3, wherein: the hydrogen peroxide is H with the mass concentration of 10 percent2O2An aqueous solution of (a).
5. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 2, wherein: the soaking time is 5-20 min.
6. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 5, wherein: the soaking time is 10 min.
7. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 2, wherein: the cleaning is carried out for 4-5 times by adopting clean water.
8. The method for removing fusarium oxysporum f.sp.cubense from bitter gourd seeds of claim 2, wherein: the seed soaking is placed in sterile water for 8-10h, and the germination accelerating temperature is 30 ℃.
9. The method for removing fusarium oxysporum f.sp.cubense of bitter gourd seeds of claim 8, wherein: the seed is soaked in sterile water for 10 h.
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Citations (7)
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|---|---|---|---|---|
| US6024986A (en) * | 1999-05-24 | 2000-02-15 | Ecolab Inc. | Method of protecting growing plants from the effects of plant pathogens |
| KR20020079478A (en) * | 2001-04-06 | 2002-10-19 | 삼성에버랜드 주식회사 | Inhibitor of lawn-disease containing hydrogen peroxide |
| MD3113G2 (en) * | 2006-02-08 | 2007-05-31 | Институт Химии Академии Наук Молдовы | Compound possessing fungitoxic activity towards the fungus Fusarium oxysporum Snyd. et Hans |
| CN103060322A (en) * | 2013-01-28 | 2013-04-24 | 广西壮族自治区农业科学院蔬菜研究所 | Fusarium oxysporum bitter gourd specialized molecular marker and application thereof |
| CN107926526A (en) * | 2017-11-20 | 2018-04-20 | 焦杰彪 | The method of one prevention watermelon blight |
| CN109757316A (en) * | 2019-01-16 | 2019-05-17 | 南京农业大学 | A kind of banana health planting system based on water disinfection |
| CN110463527A (en) * | 2019-08-21 | 2019-11-19 | 广西壮族自治区农业科学院 | The method for inducing balsam pear seedling stage anti-blight |
-
2020
- 2020-03-17 CN CN202010187348.1A patent/CN111373903A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6024986A (en) * | 1999-05-24 | 2000-02-15 | Ecolab Inc. | Method of protecting growing plants from the effects of plant pathogens |
| KR20020079478A (en) * | 2001-04-06 | 2002-10-19 | 삼성에버랜드 주식회사 | Inhibitor of lawn-disease containing hydrogen peroxide |
| MD3113G2 (en) * | 2006-02-08 | 2007-05-31 | Институт Химии Академии Наук Молдовы | Compound possessing fungitoxic activity towards the fungus Fusarium oxysporum Snyd. et Hans |
| CN103060322A (en) * | 2013-01-28 | 2013-04-24 | 广西壮族自治区农业科学院蔬菜研究所 | Fusarium oxysporum bitter gourd specialized molecular marker and application thereof |
| CN107926526A (en) * | 2017-11-20 | 2018-04-20 | 焦杰彪 | The method of one prevention watermelon blight |
| CN109757316A (en) * | 2019-01-16 | 2019-05-17 | 南京农业大学 | A kind of banana health planting system based on water disinfection |
| CN110463527A (en) * | 2019-08-21 | 2019-11-19 | 广西壮族自治区农业科学院 | The method for inducing balsam pear seedling stage anti-blight |
Non-Patent Citations (1)
| Title |
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| 韦巧婕: ""黄瓜土传枯萎病拮抗菌的筛选鉴定及其生物学效应研究"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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