KR101735258B1 - A microbial additive containing artificial soil for green roof and a method for promoting plant growth using thereof - Google Patents

Abstract

The present invention relates to a rooftop afforesting artificial soil including a microbial formulation, and to a method for producing the same. More specifically, the present invention relates to a microbial formulation including a Nocardioides sp. DAEJI-12 strain which is an actinomyces isolated from muck peat and having an excellent lignin decomposing function and an excellent antagonic ability with respect to plant pathogenic mold; a rooftop afforesting artificial soil including the same; a method for producing the same; and a method for afforesting a rooftop by promoting the growth of a plant by using the rooftop afforesting artificial soil. The rooftop afforesting artificial soil including such a microbial formulation changes physicochemical properties to be suitable for the growth of a plant, thereby promoting the growth of a plant and inhibiting plant pathogenic mold.

Description

Technical Field [0001] The present invention relates to an artificial soil for rooftop greening including microbial agents and a method for promoting growth of plants using the same.

The present invention relates to a rooftop greenery artificial soil containing a microorganism preparation and a method for producing the same. More particularly, the present invention relates to a microorganism-containing microorganism containing a strain of Nocardioides, DAEJI-12, The present invention relates to a method for greening a rooftop by promoting the growth of a plant by using an artificial soil for rooftop greening and a method for producing the same.

The rooftop greening is to create an artificial topography and a geological soil layer on the artificial structure, to plant the plants and to create the green space by creating water spaces. In addition to the aspect of utilizing the artificial earth to secure the insufficient green space in the urban area, It has attracted much attention as an alternative to increase the amount of green space in the city where it is difficult to secure an additional area. Recently, the eco-area ratio that has been applied in Eunpyeong New Town is getting more attention because it includes rooftop greening. If the rooftop greening has the same meaning as the landscape on the artificial ground, the rooftop landscaping is included in the landscaping on the artificial ground in a narrow sense (Kim, Jun-yeon, Korea Space Design Journal, Vol. Its origins can be found in Babylonia's Hanging Garden 600 years ago. As a modern concept, in the nineteenth century Germany began to cover soil for fire prevention. It was the start of artificial rooftop gardens as a means of raising sales at a department store in the UK after World War II, and the 'Heavenly Garden' of the Kyungdong boiler building in Bundang started in 1998 (Lee, Duk, The rooftop greening of the urban area, which is lacking greenery, improves the ecosystem of the city by improving the urban environment, reducing radiant heat, reducing heat, mitigating the heat island phenomenon, preventing excessive drying, , Mitigating storm drainage, meeting aesthetic demands, meeting psychological, physiological and ecological needs, environmental education, and inducing visits by outsiders. Comparing the rooftop greening of Korea with the rooftop greening of Japan, the area of rooftop greening of Seoul in Korea is very low compared to that of Tokyo, but the growth rate of the area of composition is rapidly increasing through the realization of various systems and policies This is considered to be an effort to overcome the overall dissatisfaction with the rooftop greening level, such as the rooftop greening area. In particular, it has been suggested that Korea is preferred to mixed roots and japanese people prefer rooftop greening, and it is suggested that both countries should be created for the purpose of human use (Jang, Sungwang et al., Journal of Korean Society for Environmental Restoration and Green Technology 11 ). In recent years, studies have been actively conducted on lightweight soil compositions for rooftop greening to reduce the load on the rooftops. Korean Patent Registration No. 10-0979252 discloses that the soil composition of a general rooftop greening system is different from the composition of the drainage soil and the growing soil and the particle size is controlled to be different from that of the general soil and conventional artificial soil to increase the coefficient of permeability Discloses a lightweight soil composition for rooftop greening and a rooftop greening system using the same, which can prevent loss of soil layer during intensive rainfall and increase plant availability, thereby maintaining plant growth in severe dry season. However, in order to construct fast and robust vegetation that is important in rooftop greening, techniques for manufacturing rooftop greening artificial soil containing microbial agents that promote plant growth are insufficient. The present inventors have applied a microorganism preparation to artificial soil which can be effectively used for rooftop greening based on a large amount of research experience on microbial agents that promote plant growth.

Korean Registered Patent No. 10-0979252, Aug. 31, 2010 Announcement

 Kim, Jun - Yeon, Present Status and Tasks of Roofing Greening for Environment - Friendly Urban Construction, Journal of Korean Society of Spatial Design, Vol.3, No.1, 2008, pp. 77-85  Lee, Ji-Deok, A Study on Activation of Rooftop Greening, Dept. of Space and Environmental Engineering, Kyungwon University, 2011. 12.  Jang, Sungwan, et al., Analysis and comparison of rooftop greening trends between Korea and Japan, Journal of Korean Society of Environmental Restoration, Vol. 11, No. 6, 2008, pp. 143-152

The present invention has been made to solve the problems of the prior art as described above, and it is an object of the present invention to provide a method for producing a microorganism preparation by isolating a microorganism having an antagonistic ability against a pathogenic fungus, It is an object of the present invention to provide a method for producing a rooftop artificial soil for rooftop greening and promoting the growth of plants using the same.

In order to achieve the above object, the present invention provides a method for producing lignin, which is capable of decomposing lignin under conditions of artificial soil having a large amount of components, and is capable of growing in a microorganism having excellent antagonistic ability against pathogenic fungi ( Nocardioides sp. DAEJI-12) strains of E. coli. In addition, the present invention provides a microorganism preparation for rooftop greening, wherein the DACJ-12 strain of the genus Nocardioides is cultured and contained as an active ingredient. The present invention also contemplates the production of artificial soils suitable for rooftop greening by further improving the properties of artificial soils by additionally including the microorganism preparations in an artificial soil composition containing peat, pearlite, coco peat, peat moss and zeolite. In addition, the present invention provides a method for recording a rooftop by accelerating the growth of a plant using the microorganism preparation or the artificial soil for rooftop greening comprising the same.

As described above, the microorganism preparation containing the Nocardioides -derived DAEJI-12 strain isolated from the peat of the present invention decomposes the organic matter, which is a decomposable substance, to help root planting of the plant and prevent various diseases such as fungi The artificial soil containing the microbial agent not only promotes the growth of plants but also contributes to the establishment of the basis for the ecological growth of plants and trees that are planted in the rooftop greening, It is expected to be possible.

1 shows the phylogenetic tree of 16S rDNA analysis of Nocardioides' DAEJI-12 strain isolated from peat.
Fig. 2 shows the antagonistic ability of the Norcadioides genus DAEJI-12 to the phytopathogenic fungus.
Fig. 3 shows the effect of growth of ginseng on the control.
FIG. 4 shows the effect of growth on the artificial soil without microbial agent.
Fig. 5 shows the effect of growth of the mulberry leaf on the rooftop greening artificial soil including the microbial agent.
Fig. 6 shows the effect of growth of giraffe on control.
Fig. 7 shows the growth effect of giraffe on an artificial soil not containing a microbial agent.
Fig. 8 shows the growth effect of giraffe on artificial soil for rooftop greening including microbial agents.

The present invention provides a strain of Nocardioides, DAEJI-12, which is capable of decomposing degradable organic substances such as lignin and is isolated from peat having an antagonistic effect on the pathogenic fungi of plants. The microbial formulation containing the Nocardioides genus DAEJI-12 of the present invention has properties that change the physico-chemical properties as well as the biological properties of the soil. Also, the artificial soil for rooftop greening containing the microbial agent has the effect of promoting the growth of plants such as matsumongdong and giraffe. The artificial soil for rooftop greening using the microorganism preparation containing 12 Saccharomyces cerevisiae DAEJI strain of the present invention was prepared by mixing peat, perlite, coco peat, peat moss, zeolite and microorganism preparation with 20: 40: 30: 5: 4: 1 (v / v) and 15: 50: 25: 5: 4: 1 (v / v) as an active ingredient. The microbial preparation according to the present invention may be prepared in the form of a liquid fertilizer, and may be added in the form of a powdered powder or may be granulated by formulating it into a powdery fertilizer. However, the formulation is not particularly limited. Hereinafter, preferred embodiments of the present invention will be described in detail with reference to specific examples. However, the scope of the present invention is not limited to these embodiments.

Isolation and Identification of DACJI-12 Strains from Nocardioides

Bacto peptone (5 g), Bacto yeast extract (1 g), Fe (III) citrate (1 g), and Fe (III) citrate were added to the soil or sample to separate the microorganisms which can grow under high organic conditions containing lignin component from peat, 0.1, MgCl ₂ (dried) 5.9 g, Na ₂ SO ₃ 3.24 g, CaCl ₂ 1.8 g, KCl 0.55 g, Na ₂ CO ₃ 0.16 g, KBr 0.08 g, SrCl ₂ 34 mg, H ₃ BO ₃ 22 mg, Na- , 4 mg of NaF ₂ , 1.6 mg of (NH ₄ ) NO ₃ , 8 mg of Na ₂ HPO ₄ , 33 g of NaCl and 15 g of agar were placed in 1 L of distilled water and sterilized at high temperature. And cultured at 37 ° C for 24 hours to select strains having respective characteristics. Among the microbial strains selected from the above, DAEJI-12 strain having antagonistic ability against the organic matter-degrading ability and pathogenic fungi was confirmed by the subculture and the glycerol stock was stored for the next experiment . The nucleotide sequence of 16S rDNA of the selected strains was analyzed according to a conventional bacterial 16S rDNA analysis method. A BLAST (Basic Local Alignment Search Tool) analysis of the 16S rDNA sequence (SEQ ID NO: 1) of the DAEJI-12 strain obtained through the nucleotide sequence analysis revealed that it belonged to the Nocardioides sp. 1). The Norcadioides DAEJI-12 strain has the following physiological and chemical properties as actinomycetes (see Tables 1 and 2).

Morphological characteristics of DACJI-12 strain in Norcadioides Morphological characteristics result Current classification Domain: Bacteria Phylum: Actinobacteria Class: Actinobacteria Order: Actinomycetales Family: Nocardioidaceae Genus: Nocardioides Species: Nocardioides lutues Similairy: 99% G + C High Gram stain Positive Cell shape Rod shaped Motility Not motile Sporulation Nonsporulating Temperature range Mesophile Optimum temperature 37 ° Salinity 0 to 1% Oxygen requirement Aerobic Biotic relationship Free-living Depth / Sample Surface / Soil

Physiological and biochemical characteristics of DACJI-12 strain of Norcadioides Physiochemical properties DAEJI -12 strains Cell diameter (μm) 0.30.8 x 60.91.4 Oxygen requirement Aerobic Gram stain + Motility Endospore formation Production of:  Alkaline phosphatase +  Acid phosphatase +  Catalase +  Oxidase  Nitrate reductase  Urease  α-Galactosidase  β-Galactosidase  β-Glucuronidase  α-Glucosidase  β-Glucosidase  Esterase +  Esterase lipase  Naphthol-AS-BI-phosphohydrolase +  N-acetyl-β-glucosaminidase  α-Mannosidase  α-Fucosidase  Leucine arylamidase +  Valine arylamidase  Cystine arylamidase  α-Chymotrypsin  Trypsin Utilization of:  5-Keto-gluconate NA  d-Xylose +  d-Fructose  d-Glucose +  d-Mannose + +, positive; , negative; w, weakly positive; NA, data not available.

Antagonistic Analysis of the Novel Antagonistic Microorganism Nocardioides in DAEJI-12

 Eight plant pathogenic fungi were distributed at the Korean Agricultural Microorganism Resource Center (KACC) to confirm the antagonistic action of the Nocardioides of the present invention DAEJI-12. In the R2A plate medium, the antagonistic microorganism Nocardioides of the present invention, DAEJI-12, and eight phytopathogenic fungi were cultured in the shade for 5 days at 28 DEG C for growth confirmation. A description of the phytopathogenic fungi and the results of the antagonistic action of DADJI-12 on the plant pathogenic fungus in the genus Norcadioides is as follows. Colletotrichum gloeosporioides, the first phytopathogenic fungus, is an anthrax anthracnose (different from anthrax of an animal) that causes anthrax of a plant. Anthracnose of plants is pepper, rice, bean, cucumber , Asteraceae, and persimmon such as persimmon, plum tree, peach tree, citrus tree, chestnut tree, and apple tree. Damaged crops include pepper, rice, soybeans, magpie, pea, wheat, spinach, cucumber marsh, and other crops. Any brown or dark brown spots on the leaves, stems, and fruit can cause deciduous leaves and fallen leaves. As the lesion gradually spreads and humidity increases, reddish brown or pink spores of conidia form on the lesion. It usually becomes an infectious source after wintering in the form of a conidium (small black spot) made in the lesion, and the disease rate is high when the temperature and humidity are high. Referring to the photograph (A) in Fig. 2, it can be seen that Nocardioides' DAEJI-12 (left) shows antagonism to choletotrysin gene eosporioides (right). The characteristic of the wilt caused by the second plant pathogenic fungus Fusarium oxysporum is high temperature drying, which survives for several decades in the soil, causing seed infestation and invading the ducts of crops, causing wilting symptoms. It occurs very much even when there is a lot of nitrogenous fertilizer. The wilt disease occurs in August with a high temperature and high humidity, and the stem is dry due to severe symptoms in the middle of the year. Referring to FIG. 2 (B), it can be seen that the Nocardioides genus DAEJI-12 (left) shows an antagonistic action on the fuertum ooxis forum (right). The third phytopathogenic fungus, Rhizoctonia solani, is the causative agent of melancholia. Melancholy is a disease caused by microbial infestation of the bottom of the mock or root after the seed is sprouted, and the end of the mock suddenly dries up or dies as a needle. Melancholia is the main disease of the tombs caused by the combination of Laryngo, Pisium, and Pseudomonas. It develops in low temperature and high humidity condition and does not develop if it grows a little more. Referring to the photograph (C) in FIG. 2, it can be seen that the Nocardioides genus DAEJI-12 (left) shows an antagonistic effect on the Lyocalonia solani (right). Scleroderma, the causative organism of Sclerotinia sclerotiorum, a fourth plant pathogenic fungus, occurs in fruits such as pear, apple, loquat, peach, plum and apricot. It occurs mainly in fruit, but may also occur in branches and flowers. Brownish spots on the surface of the fruit grow and grow on the surface, with a brownish white or cream colored spore lump with a rounded pattern. After that, sick fruity rotates and emits a strong smell. Pathogens are largely mycelial or spore-shaped, and they grow on the diseased part of the diseased fruit or tree branches and become the first infectious source of the next year. However, the pericardium is formed in sclerotia adhered to fruit buried in the ground, It is also transmitted by. Referring to the photograph (D) in FIG. 2, it can be seen that the Nocardioides genus DAEJI-12 (left) shows antagonism to sclerotinia sclerotiorum (right). The fifth plant pathogenic fungus, Rhizopus stolonifer, causes blight. When it gets cloudy, the pectin in the middle layer of the cell wall melts, and at first it looks like water, but it gradually recovers and rotates and becomes like liquid. Rootstocks and tubers, stems in the ground, petioles, etc. are first infested. Then gradually the leaf is infested. When the petiole gets blighted, the leaf becomes yellow and sagged and falls off. It is a disease that appears in many vegetables, and it also appears in tomatoes, sweet potatoes and potatoes. Depending on the breed, the pathogens are different and can be seen in the ground with the residues of the plant near the root of the host plant or weed. It is transmitted by pests or contaminated soil, and sometimes by plant wounds and water holes. Referring to the photograph (E) in FIG. 2, it can be seen that the Nocardioides subsp. DAEJI-12 (left) shows an antagonistic action on the Rhizopus stolonifera (right). The gray mold disease caused by Botrytis cinerea, the sixth phytopathogenic fungus, occurs in potato leaves, potatoes, peppers, apple trees and ginseng roots, and occurs in the grounds such as fruit, calyx, The damage mainly appears on the site. Especially, it causes great damage to the fruit, and it breaks into the young fruit, and when it browse, black browns and humid, it becomes a rotten, grayish pathogen. In the case of all varieties, when the petals do not fall off after fertilization, the buds turn red when browned, browning, black browning and rotting. It is assumed that the pathogen is parasitic on the dead part of the lower leaf at first, and it is transmitted by the spore. Gray mold fungus penetrates through the organs of flowers, such as wound area, petals, pistil, and surgery, which are very weak and have a weak attack on the healthy tissue by spores. It is attached to the body of pollen-mediated bee and spreads through flowers. Referring to the photograph (F) in FIG. 2, it can be seen that the Nocardioides genus DAEJI-12 (left) shows an antagonistic action on Vortis thincteria (right). Phytophthora capsici, a seventh phytopathogenic fungus, is a pandemic of crops caused by airborne infections. Leaves have dark green spots and white fungus. When dried, they become brown and can be seen in potatoes, tobacco, tomatoes. Referring to photograph (G) in FIG. 2, it can be seen that DACJI-12 (left) in the genus Nocardioides shows an antagonistic action on the phytosphorosaccharide (right). Sclerotium rolfsii, the last eighth plant pathogenic fungus, causes white silkworm disease. When you dig the upper part of the culture soil, you can see that the yarn-like hyphae of shiny white are clogged between the roots. Mycelium and sclerotia are transmitted through the soil. The infected trees and grass are covered with pollinated hyphae. Later, dark brown sclerotia appeared, and it seemed to be smaller than red squash and red seeds. Soon the roots start to rot, the leaves wilt, and the whole plant dries up dead. Referring to the photograph (H) in FIG. 2, it can be seen that the Nocardioides genus DAEJI-12 (left) shows antagonism to Sclerotium rufus (right). As can be seen in Table 3, it was confirmed that the Nocardioides genus DAEJI-12 of the present invention was antagonistic to all of the eight phytopathogenic fungi.

Antagonism (+, antagonistic activity) of Nocardioides genus DAEJI-12 to phytopathogenic fungi
No.
Plant pathogenic fungus name
DAEJI-12 antagonism
One Colletotrichum gloeosporioides
+
2
Fusarium oxysporum
+
3
Rhizoctonia solani AG-1 ( IA )
+
4
Sclerotinia sclerotiorum
+
5 Rhizopus stolonifer there . stolonifer
+
6 Botrytis cinerea
+
7
Phytophthora capsici
+
8
Sclerotium rolfsii
+

As a result of analyzing the morphological, physiochemical and molecular biological classification characteristics of the DAEJI-12 strain, the strain was identified as a new strain belonging to the genus Nocardioides sp., And was identified as Nocardioides DAEJI-12), which was deposited on October 7, 2016 at KCTC (Korea Research Institute of Bioscience and Biotechnology) and deposited with KCTC 18503P (see attached deposit note) ).

Production of microorganism preparation through mass culture of DACJI-12 strain of Nocardioides

In order to prepare the microorganism preparation containing the strain DAEJI-12 isolated in the present invention, the microorganism strain of the genus Nocardioides was added with 0.1% of lactose, 0.01% of mineral and 0.01% of molasses. As a result, it was able to grow at various temperatures, especially at 30 ~ 35 ℃. A liquid microbial formulation containing the culture of the large-scale cultured DAEJI-12 strain was prepared and subsequently added as an active ingredient of an artificial soil containing various lightweight materials.

Analysis of characteristics of various lightweight disclosure materials and production of artificial soil for rooftop greening

The physicochemical properties of heavy metals such as peat, pearlite, coco peat, peat moss and zeolite, and heavy metals and harmful component contents were analyzed and used as a medium for rooftop greening artificial soil. As a result of analysis, heavy metals and harmful components showed low contents and nutrients needed for plant growth could be supplied. (See Tables 4 and 5)

Analysis of Physicochemical Properties of Dispersion Materials division pH
(1: 5) EC T-N O.M Ava .-
P ₂ O ₅ Ex .- cations
( cmol _c / kg ) CEC moisture volume
density dS / m % mg / kg Ca ^{2 +} Mg ^{2 +} K ⁺ Na ⁺ cmol _c / kg % Mg / m ³ peat 3.52 5.74 0.51 14.3 trace 11.1 2.54 0.32 0.17 30.3 55.3 0.64 Pearlite 7.52 0.04 0.12 0.71 trace 0.17 trace 0.07 0.22 16.2 24.1 0.18 Coco pit 5.84 3.81 0.58 47.0 245 11.4 5.05 14.5 4.12 42.1 29.4 0.14 Peatmoss 7.52 0.02 0.13 0.72 trace 0.21 trace 0.05 0.21 16.4 24.2 0.16 Zeolite 7.54 1.22 0.01 4.73 60.1 23.4 12.5 14.4 11.3 63.1 15.5 1.03

Analysis of Heavy Metals and Hazardous Substance Content of Disclosure Materials division As CD Hg Pb Cr ^{6 +} Cu Ni Zn salt mg / kg % peat trace trace trace 11.67 58.00 18.40 27.30 57.10 0.01 Pearlite trace trace trace 0.63 trace trace trace 5.50 0.01 Coco pit trace trace trace trace trace trace trace 19.40 0.40 Peatmoss trace trace trace 3.74 2.10 1.60 1.28 15.30 0.04 Zeolite trace trace trace 6.30 2.09 0.70 1.20 64.21 0.61

To prepare artificial soil for rooftop greening, six types of feedstuffs were set as shown in Table 6, and the physicochemical properties and harmful components were analyzed.

Ratio of input of artificial soil material for rooftop greening (v / v) division One 2 3 4 5 6 peat 15 20 25 15 20 25 Pearlite 45 40 35 50 45 40 Coco pit 30 30 30 25 25 25 Peatmoss 5 5 5 5 5 5 Zeolite 4 4 4 4 4 4 Microbial agent One One One One One One

Analysis of physicochemical properties according to the input ratio of artificial soil material for rooftop greening division pH
(1: 5) EC T-N O.M Ava .-
P ₂ O ₅ Ex .- cations
( cmol _c / kg ) CEC moisture Bulk density dS / m % mg / kg Ca ^{2 +} Mg ^{2 +} K ⁺ Na ⁺ cmol _c / kg % Mg / m ³ One 4.10 2.92 0.25 22.5 66.3 7.95 12.4 5.57 3.71 37.0 14.8 0.26 2 4.05 3.07 0.27 23.7 65.4 6.02 12.1 5.02 2.80 44.0 17.5 0.31 3 3.91 3.32 0.27 25.4 65.2 5.59 11.7 4.95 2.72 47.0 21.4 0.37 4 4.21 3.08 0.25 19.2 54.2 7.02 12.2 5.20 3.40 32.4 14.0 0.27 5 4.11 3.50 0.27 20.2 54.8 6.83 11.1 5.11 3.05 34.5 16.1 0.32 6 3.95 3.70 0.27 22.3 54.6 5.06 11.7 4.70 2.48 38.2 20.4 0.37

Analysis of Hazardous Substance Content by Ratio of Raw Material Input for Rooftop Greening division As CD Hg Pb Cr ^{6 +} Cu Ni Zn salt mg / kg % One trace trace trace 0.99 20.2 3.22 11.50 14.2 0.51 2 trace trace trace 1.01 22.3 4.06 12.30 17.2 0.35 3 trace trace trace 1.14 24.5 5.23 13.51 21.0 0.24 4 trace trace trace 1.05 17.7 1.90 10.04 13.0 0.49 5 trace trace trace 1.20 19.0 2.50 11.20 15.6 0.30 6 trace trace trace 1.41 20.5 3.45 12.10 19.5 0.21

As shown in Tables 7 and 8, peat, pearlite, coco peat, peat moss, zeolite and microbial composition were mixed at 20: 40: 30: 5: 4: 1 (v / v) and 15: 50: 25: 5: 4: 1 (v / v). 2 and No. 4 was suitable for rooftop greening artificial soil. To compensate for the low pH of the artificial soil, pH was measured at 20 days after lime treatment at 0.1% by volume (Table 6). The pH values were 6.45 and 6.30, respectively, suitable for microbial vegetation. Therefore, the artificial soil No. 1 for rooftop greening. 2 and No. 4 was used for the cultivation of maekmundong and girinchos. The artificial soil for rooftop greening was treated with 0.1% (v / v) of lime and 20 days later.

Control (K-media) and artificial soil for rooftop greening. Analysis of Physicochemical Properties of 2, 4 division pH
(1: 5) EC T-N T-P T- Ca T- Mg T-K Organic matter moisture dS / m % K-media ^* 6.06 2.91 0.20 0.06 0.21 0.15 0.50 23.70 49.50 2 for rooftop greening 6.45 3.40 0.25 0.09 0.27 0.27 0.52 25.00 24.10 For rooftop greening 4 6.30 3.02 0.26 0.05 0.32 0.31 0.62 19.20 22.90

 * K-media compounding ratio = coco peat: peat moss: vermiculite: pearlite = 55: 15: 15: 15 (v / v)

Changes in chemical properties of artificial soil for rooftop greening including microbial agents

In order to evaluate the physicochemical characteristics of the artificial soil for rooftop greening including the liquid microorganism preparation prepared in the present invention and the effect of promoting the growth of the plants, Were carried out. In order to evaluate the plant growth promoting effect of the artificial soil for rooftop greening including the microbial preparation prepared according to the present invention, the standard of the port per treatment was performed by repeating the random arrangement method in the vinyl house with a square port of 1: Crops were performed over 1 week. K-media was used as a control at a mixing ratio of coco peat: peat moss: vermiculite: pearlite = 55: 15: 15: 15 (v / v). For rooftop greening, I and II are green soils for rooftop greening without microbial agents. 2 and No. 4 for rooftop greening, and I-M and II-M for rooftop greening, respectively. 2 and No. 4.

Changes in chemical properties of artificial soil for rooftop greening by cultivation process  phrase pH
(1: 5) EC T-N O.M Ava .-
P ₂ O ₅ Ex .- cations
( cmol _c / kg ) CEC dS / m % mg / kg Ca ^{2 +} Mg ^{2 +} K ⁺ Na ⁺ cmol _c / kg Control 5.30 1.92 0.14 15.7 42.1 7.14 9.42 3.42 2.15 21.0 Rooftop greening Ⅰ 5.80 2.31 0.22 18.1 48.1 7.32 10.10 4.12 2.10 22.5 Rooftop greening Ⅰ-M 5.85 2.33 0.24 17.4 49.5 7.40 10.20 4.16 2.12 23.0 Rooftop greening Ⅱ 5.68 2.20 0.22 17.0 45.2 7.45 10.41 4.21 2.16 23.2 Ⅱ-M for rooftop greening 5.75 2.25 0.24 16.6 46.2 7.50 10.55 4.24 2.21 23.4

Changes in Chemical Properties of Artificial Soils for Rooftop Greening by Kirinchos Cultivation process  phrase pH
(1: 5) EC T-N O.M Ava .-
P ₂ O ₅ Ex .- cations
( cmol _c / kg ) CEC dS / m % mg / kg Ca ^{2 +} Mg ^{2 +} K ⁺ Na ⁺ cmol _c / kg Control 4.90 2.00 0.16 14.7 41.4 7.03 9.95 4.01 1.70 23.5 Rooftop greening I 5.42 2.51 0.20 15.2 39.1 7.35 10.15 4.08 1.82 24.4 I-M for rooftop greening 5.50 2.70 0.21 15.0 40.4 7.50 10.21 4.15 1.83 24.6 For rooftop greening II 5.21 2.40 0.23 14.5 37.3 7.75 10.25 4.20 1.85 25.0 II-M for rooftop greening 5.35 2.42 0.25 13.9 38.0 7.91 10.27 4.23 1.94 25.2

As shown in Table 10 and Table 11, the chemical characteristics of the artificial soil for rooftop greening according to the cultivation of McMundong and Kirinchos showed that the pH, nitrogen content, available phosphoric acid and cation And the contents were high. The pH, nitrogen, and cation content of the roots were increased by the addition of the microbial agent to the rooftop greening artificial soil. The nitrogen content and the substitutional cation content of the artificial soil for rooftop greening were significantly Respectively.

Changes in physical properties of artificial soil for rooftop greening including microbial agents

Changes in physical properties of artificial soil for rooftop greening due to cultivation process  phrase Bulk density Solid phase Liquid phase weather Porosity Mg / cm ³ % Control 0.24 9.2 56.2 34.6 90.8 Rooftop greening I 0.20 7.7 56.5 35.8 92.3 I-M for rooftop greening 0.18 7.0 59.7 33.3 93.0 For rooftop greening II 0.17 6.6 60.3 33.1 93.4 II-M for rooftop greening 0.16 6.2 60.6 33.2 93.8

Changes in Physical Properties of Artificial Soils for Rooftop Greening by Kirinchos Cultivation process  phrase Bulk density Solid phase Liquid phase weather Porosity Mg / cm ³ % Control 0.21 8.0 60.2 31.8 92.0 Rooftop greening I 0.18 6.9 66.0 27.0 93.1 I-M for rooftop greening 0.17 6.5 68.9 24.6 93.5 For rooftop greening II 0.17 6.6 70.4 23.0 93.4 II-M for rooftop greening 0.16 6.1 71.9 21.9 93.9

Table 12 and Table 13 show that the physical properties of the artificial soil for rooftop greening due to the cultivation of McMundong and Kirinchos showed a tendency that the volume density of soil treated with rooftop greening artificial soil was lower than that of the control soil , And the porosity was the highest for the rooftop greening Ⅱ-M treatment.

Plant growth promotion effect of artificial soil for rooftop greening including microbial agent

Growth and fresh weight characteristics of ginseng root according to artificial soil for rooftop greening Treatment center Super sheet Leaf number Leaf lock Fresh weight Length
(cm) Indices
(%) Count
( ea /week) Indices
(%) content
( mg / 100cm ² ) Indices
(%) weight
(g / week) Indices
(%) Control 29.8 ^{* a} 100 37.0 ^a 100 5.87 ^a 100 11.1 ^a 100 Rooftop greening I 32.3 ^a 108 39.7 ^a 107 6.17 ^a 105 11.9 ^a 107 I-M for rooftop greening 33.0 ^a 111 40.3 ^a 109 6.25 ^a 107 12.1 ^a 109 For rooftop greening II 33.4 ^a 112 41.3 ^a 112 6.23 ^a 106 12.4 ^a 112 II-M for rooftop greening 36.5 ^a 123 44.7 ^a 121 6.33 ^a 108 14.0 ^a 126

* Duncan Shinda Black 5% level

According to the above Table 14, plant height and leaf length were increased by 8 ~ 23% and 7 ~ 21%, respectively, compared to the control (see Figs. 3 and 4), and the microbial growth And 23% and 21%, respectively, for a rooftop greening Ⅱ-M treatment. The fresh weight of the roots was also increased by 7 ~ 26% higher than that of the control (see FIG. 5).

Growth and Biomimetic Properties of Giraffe Preparations by Artificial Soil for Roofing Treatment center Super sheet Leaf number Leaf lock Fresh weight Length
(cm) Indices
(%) Count
( ea /week) Indices
(%) content
( mg / 100cm ² ) Indices
(%) weight
(g / week) Indices
(%) Control 21.8 ^{* a} 100 147.8 ^a 100 5.16 ^a 100 52.3 ^a 100 Rooftop greening I 23.4 ^a 107 156.3 ^a 106 5.39 ^a 105 57.1 ^a 109 I-M for rooftop greening 23.8 ^a 109 157.7 ^a 107 5.51 ^a 107 57.8 ^a 110 For rooftop greening II 24.2 ^a 111 161.5 ^a 109 5.47 ^a 106 58.4 ^a 112 II-M for rooftop greening 26.5 ^a 122 166.7 ^a 113 5.62 ^a 109 62.7 ^a 120

* Duncan Shinda Black 5% level

As shown in Table 15, the growth rate of the Kirinchosho was increased by 7 ~ 22% and 6 ~ 13%, respectively, when the plant height and leaf number were higher than that of the control (Figure 6, 7 And 22% and 13%, respectively, for the rooftop greening treatment containing microorganisms, respectively, and the live weight was also increased by 9 to 20% higher than the control (see FIG. 8). During the cultivation period, there was no growth damage or composting in the mulmongdong as well as the giraffe. The artificial soil for rooftop greening containing microbial agents containing Nocardioides sp. DAEJI-12 strain, DACJI-12, which is excellent in organic matter resolution and antagonistic to fungal antibiotics, , And the growth of plants such as mulmongdong and girinchus was promoted in comparison with artificial soil which did not contain artificial soil. Also, the physico-chemical properties of artificial soil were changed to create an environment suitable for plant growth, It is expected.

Korea Biotechnology Research Institute KCTC18503P 20161007

Nocardioides sp. DAEJI-12 16S rDNA (1428 bp)
gtcccgcaacgagcgcaaccctcgtcctatgttgccagcaattcggttggggactcataggagactgccggggtcaactcggaggaaggtggggatgacgtcaagtcatcatgccccttatgtccagggcttcacgcatgctacaatggccggtacaaagggctgcgatcccgtgagggtgagcgaatcccaaaaagccggtctcagttcggattggggtctgcaactcgaccccatgaagtcggagtcgctagtaatcgcagatcagcaacgctgcggtgaatacgttcccgggccttgtacacaccgcccgtcacgtcacgaaagtcggcaacacccgaagccagtggcccaacccttgtggggggagctgtcgaaggtggggctggcgattgggacgaagt

<110> DAEJI Development Co., Ltd <120> A microbial additive containing an artificial soil for vegetable          gardens for the revitalization of sustainable urban agriculture <130> KCTC18503P <160> 1 <170> KoPatentin 3.0 <210> 1 <211> 1428 <212> DNA <213> Nocardioides sp. <400> 1 cggcgtgctt aacacatgca agtcgagcgg caaggccctt cggggtactc gagcggcgaa 60 cgggtgagta acacgtgagt aatctgcccc aggctctggg atagccaccg gaaacggtga 120 ttaataccgg atacgacaac cgattgcatg atctggttgt ggaaagtttt tcggcctggg 180 atgtgctcgc ggcctatcag cttgttggtg aggtaatggc tcaccaaggc ttcgacgggt 240 agccggcctg agagggtgac cggccacact gggactgaga cacggcccag actcctacgg 300 gaggcagcag tggggaatat tggacaatgg gcggaagcct gatccagcaa cgccgcgtga 360 gggatgacgg ccttcgggtt gtaaacctct ttcagcacag acgaagcgaa agtgacggta 420 tgtgcagaag aaggaccggc caactacgtg ccagcagccg cggtaatacg tagggtccga 480 gcgttgtccg gaattattgg gcgtaaaggg ctcgtaggcg gtctgtcgcg tcgggagtga 540 aaaccaggtg cttaacacct ggcctgcttt cgatacgggc agactagagg tactcagggg 600 agaatggaat tcctggtgta gcggtgaaat gcgcagatat caggaggaac accggtggcg 660 aaggcggttc tctgggagta tcctgacgct gaggagcgaa agtgtgggga gcgaacagga 720 ttagataccc tggtagtcca caccgtaaac gttgggcgct aggtgtggga tccattccac 780 gggttccgtg ccgcagctaa cgcattaagc gccccgcctg gggagtacgg ccgcaaggct 840 aaaactcaaa ggaattgacg ggggcccgca caagcggcgg agcatgcgga ttaattcgat 900 gcaacgcgaa gaaccttacc tgggtttgac atacaccgga aagctgcaga gatgtagccc 960 cttttagtcg gtgtacaggt ggtgcatggc tgtcgtcagc tcgtgtcgtg agatgttggg 1020 ttaagtcccg caacgagcgc aaccctcgtc ctatgttgcc agcaattcgg ttggggactc 1080 ataggagact gccggggtca actcggagga aggtggggat gacgtcaagt catcatgccc 1140 cttatgtcca gggcttcacg catgctacaa tggccggtac aaagggctgc gatcccgtga 1200 gggtgagcga atcccaaaaa gccggtctca gttcggattg gggtctgcaa ctcgacccca 1260 tgaagtcgga gtcgctagta atcgcagatc agcaacgctg cggtgaatac gttcccgggc 1320 cttgtacaca ccgcccgtca cgtcacgaaa gtcggacaaca cccgaagcca gtggcccaac 1380 ccttgtgggg ggagctgtcg aaggtggggc tggcgattgg gacgaagt 1428

Claims (7)

Colletotrichum gloeosporioides , a causative organism of plant anthracnose, Fusarium oxysporum , a causative agent of wilt disease , Rhizoctonia solani , a causative organism of constipation, Rhizoctonia solani , Sclerotinia sclerotiorum , Rhizopus stolonifer , causative agent of rot cause, Botrytis cinerea , causative agent of gray mold , Pitot, The phytopathogenic fungi belonging to the family Phytophthora capsici and the phytopathogenic fungi consisting of Sclerotium rolfsii which is the causative agent of the white nasopharyngeus, Nocardioides isolated from peat having excellent lignin-resolving ability DAEJI-12 strain (KCTC 18503P) A microorganism preparation for rosette greening comprising the Nocardioides genus DAEJI-12 strain (KCTC 18503P) according to claim 1 as an active ingredient Artificial soil for rooftop greening comprising peat, perlite, coco peat, peat moss and zeolite, comprising the microbial formulation according to claim 2 The method according to claim 3, wherein the contents of peat, pearlite, coco peat, peat moss, zeolite and microbial agent are 20: 40: 30: 5: 4: 1 (v / v) or 15: 50: 25: 1 (v / v). The present invention relates to a process for the production of lightweight dispensing materials such as peat, perlite, coco peat, peat moss and zeolite with a microbial formulation according to claim 2 in a ratio of 20: 40: 30: 5: 4: 1 (v / v) or 15: 50: 25: 5: 4 : 1 (v / v). The method for producing the artificial soil for rooftop greening A method for promoting the growth of plants by using the artificial soil for rooftop greening according to claim 3 or 4 A method for greening a rooftop using the artificial soil for rooftop greening according to claim 3 or 4

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