KR20020040374A - Method for extracting a raw material of a hibiscus syriacus - Google Patents

Abstract

PURPOSE: A process for extracting an althea is provided, which extract has strong antioxidative effect, so that it can be effectively used for inhibiting skin aging. CONSTITUTION: The process for extracting an althea comprises the steps of: drying(S2) and heating the althea at 95 to 105 deg.C(S3); drying the althea(S4) and pulverizing it(S5); dipping the pulverized althea in a mixed solvent of ethanol and deionized water in the ratio of 6:4(S6) and refluxing the mixed solvent(S7); filtering the mixed solvent containing the althea to collect the althea samples, dipping the althea samples in the same mixed solvent(S8) and refluxing the mixed solvent(S9); filtering the mixed solvent to collect the althea samples, and centrifuging the althea samples for 3 to 6 minutes to collect the supernatant(S10); and condensing the supernatant to prepare the extract of althea(S11).

Description

Method for extracting a raw material of a hibiscus syriacus}

The present invention relates to a method for extracting a certain amount of raw material from the hibiscus (Hibiscus syriacus), and more particularly, by improving some of the conventional processes, by maximizing the function of the rose of sharon, the quality of the final product is The present invention relates to a method for extracting green light, which can be improved to a level or higher.

Recently, it has been widely known that Mugunghwa performs various beneficial effects on the human body, such as "fast excretion of wastes in blood vessels", "preventing arterial passage", and "anticancer effect". A variety of researches are being actively conducted to apply this to real life.

For example, Korean Patent Laid-Open Publication No. 1997-9620, "Method for preparing a rose of sharon, containing an active bioactive substance", Korean Patent Laid-Open Publication No. 1997-9773, "Manufacturing method for lipsticks containing a pigment of rose of sharon", Korean Patent Laid-Open Korean Unexamined Patent Publication No. 1997-9774 "Method of manufacturing volcanic paper containing pigment of Mugunghwa", Korean Patent Publication No. 1997-9775 "Method of manufacturing eyeshadow containing pigment of Mugunghwa", Korean Patent Publication No. 1997-10951 " Manufacturing method of brewed vinegar based on Mugunghwa "," Korean Patent Publication No. 1997-20093 "skin cosmetics using Mugunghwa" and the like have been described in detail a variety of conventional studies for applying Mugunghwa in real life.

However, recently, as the research on Mugunghwa advances, it is gradually revealed that the conventional Mugunghwa application technology still contains many problems.

For example, in the conventional application of the green light, the raw material of the green light is extracted by using an organic solvent such as methanol, isopropyl alcohol, butanol, benzene, hexane, acetone, dichloromethane, and the like. With the controversy of being very harmful, it has been proved that the conventional Mugunghwa application still contains many problems.

In addition, in the conventional method for extracting the use of rose of sharon, for example, the conventional method of extracting cosmetic raw materials using Mugunghwa, there is no need for any additional process that can double the efficacy of Mugunghwa. Recently, it has been found that by further doubling the pharmacological effects of Mugunghwa, it is possible to reduce the harmful effects on free-radical, which is the main cause of skin aging. There is still evidence of many problems.

In the presence of such problems, for example, if the conventional method for extracting cosmetic raw materials using green light is used without any improvement, many users of "green light cosmetics" may receive a series of "skin damage risk" and "skin aging risk". "There is a serious problem that remains exposed.

Accordingly, an object of the present invention is to use a mixed solution of a concept other than organic solvents such as methanol, isopropyl alcohol, butanol, benzene, hexane, acetone, dichloromethane, etc. To eliminate the threat of skin damage.

Another object of the present invention is to enhance the antioxidant activity of the green light through a series of processing process, through which the final finished green light is effective to neutralize the effect of free radicals found to be the main cause of aging. By retaining the function, the quality of the finished product is improved to a certain level or more.

Still other objects of the present invention will become more apparent from the following detailed description and the accompanying drawings.

1 is a process flow chart sequentially showing a method for extracting the green light according to the present invention.

Figure 2 is a graph showing the antioxidant activity of the green light source extracted by the present invention.

In order to achieve the above object, in the present invention, after the first forced drying of a series of green light extracts, it is heated at a high temperature, for example, 95 ° C. to 105 ° C., and the green light extract is completed. After the second forced drying, the green light extract was pulverized, and the green light harvest was completed by first immersion in a mixed solvent mixed with ethanol and deionized water at a ratio of, for example, 60:40. After the first reflux step and the mixed solvent having completed the first reflux process are filtered, the green light is collected from the mixed solvent, and the selected green light is secondary to the above-mentioned mixed solvent. After immersion, the step of refluxing the secondary, and the mixed solvent of the completion of the secondary reflux is filtered to select the green light from the mixed solvent, centrifuged for 3 minutes to 6 minutes, the selected green light, Work Disclosed is a method for extracting the green light, which comprises the steps of extracting the supernatant of the lotus, and condensing the supernatant to collect the final green light extract.

In the present invention, as mentioned above, a series of heating process is performed on the green light collected after the first forced drying, the heat having a temperature of, for example, 95 ℃ ~ 105 ℃ is applied to the green light collected And, through this, promote tissue breakdown of the green light, thereby inducing the production of phenolic substances. Since these phenolic substances are known to possess excellent antioxidant properties, when the present invention is achieved, the green light can have a strong antioxidant activity, and eventually, the final finished product is found to be a major cause of aging free. It is possible to have an effective function to neutralize the effects of radicals.

In addition, in the present invention, as described above, using the mixed solvent other than the conventional organic solvent, for example, a mixed solvent in which the ratio of ethanol and deionized water is mixed in a ratio of 60:40, the green light is extracted. Since ethanol and deionized water are known to have almost no effect on the skin, when the present invention is achieved, the green light raw material can easily escape from the influence of the organic solvent, thus retaining excellent skin adaptability. The final finished product does not cause any problems such as damaging the skin unlike the conventional.

Hereinafter, with reference to the accompanying drawings, a more detailed description of the extract of the green light according to the present invention.

As shown in FIG. 1, in the present invention, first, a series of green light is collected from a part of the green light, for example, the root of the green light, the stem of the green light, and the like. Then, the green light is washed clean (step S1).

Subsequently, a process of first forced drying of the green light collected after washing is performed (step S2). In this case, the Rose of Sharon is subjected to the process of natural drying in the shade, or forcedly dried by a blower or low temperature hot air in the shade.

When this primary forced drying process is completed, the present invention proceeds to the process of heating the previous rose of sharon harvest (step S3). In this case, the green light is subjected to a process of, for example, heating with steam having a temperature of 95 ° C to 105 ° C, preferably with steam having a temperature of 100 ° C, for example, from 22 hours to 26 hours, preferably 24 hours.

As such, when the heating process is performed for about 24 hours, the green light undergoes a process in which its own tissue is decomposed by the heat transferred from the water vapor. Thus, a large amount of phenolic substance is formed and liberated in some of the green light. do. Since these phenolic substances are known to possess excellent antioxidant properties, when the present invention is achieved, the green light extract can have a strong antioxidant activity, and finally, the finished product based on the green light extract is It will have an effective function to neutralize the effects of free radicals, which have been found to be the main cause of aging.

On the other hand, through the above process, when a series of heating process is all finished, the process of secondary forced drying of the above-mentioned rose of sharon harvest is carried out (step S4). Even when the second forced drying process is in progress, as in the first forced drying process, the green leaves are naturally dried in the shade or forcedly dried by a blower or a low temperature hot air in the shade. Rough When the series of secondary forced drying process is progressed, the rose of sharon has a certain level of antioxidant activity reproducing ability, and in the present invention, as described above, the series of secondary forced drying process is performed in a high temperature environment. By only proceeding in a "natural drying environment", a "blower or a low temperature hot air environment", the problem that the antioxidant activity reproducing ability of the green light extract is destroyed is prevented in advance.

On the other hand, when the secondary forced drying process described above is finished, the process of grinding the green light is finely pulverized, and when the grinding process is finished, the ground green light is collected in a mixed solvent comprising a mixture of ethanol and deionized water. The first immersion process is performed (steps S5 and S6).

As described above, in the case of the present invention, the process of immersing the green light collected after the grinding process is immersed in a mixed solvent other than the conventional organic solvent such as ethanol and deionized water mixed with the mixed solvent, such ethanol and de Since ionized water is known as a substance that does not adversely affect the skin, when the present invention is achieved, the green light is easily released from the influence of the organic solvent, thereby maintaining excellent skin adaptability, and finally, The cosmetic raw material does not cause any problems such as damaging the skin unlike the conventional.

At this time, in the present invention, ethanol and deionized water are mixed at a ratio of, for example, 60:40. This mixing ratio of ethanol and deionized water is a very important matter in achieving the present invention effectively. If the mixed solution does not maintain the above 60:40 ratio, for example, when the ratio of deionized water becomes higher than necessary compared to ethanol, other types of antioxidants are produced in addition to the phenolic substances. Although this can be obtained, in this case, another problem may arise, in which a large amount of other substances are produced that may adversely affect the skin, on the contrary, when the ratio of ethanol becomes higher than necessary, low polar substances As a result of mass production, these low-polarity substances act as a cause for preventing the smooth production of phenolic substances, which may cause a problem of lowering the antioxidant activity of the green light extract. In view of the above problems, the present invention sufficiently maintains the mixing ratio of ethanol and deionized water constituting the mixed solution at about 60:40 as described above, so that the antioxidant activity of the green light can be maintained in an optimal state. .

At this time, in the present invention, the mixed solvent is immersed in the mixed solvent to maintain the volume of 4ml per 1g of the green light. That is, in the present invention, when the mixed solvent is 20 ml, for example, 5 g of the green light is immersed.

Of course, by increasing the amount of the mixed solvent for the same amount of green light extract, it is possible to obtain a more effective extract of the green light, but in this case, it may cause a problem that the cost is more than necessary. In view of the above problems in the present invention, by maintaining the amount of the mixed solvent at about 4mml per 1g of the green light, so that more than a certain level of cost, the extract of the green light can be maintained more than a certain level.

On the other hand, when the above-mentioned primary immersion process is completed, the mixed solution in which the green light is immersed is first refluxed for 3 hours, for example, to extract a certain amount of the green light (Step S7). By the reflux process, the loss of unnecessary mixed solvent is blocked in advance, and a certain ratio of the extract of the green light is ensured.

When the first reflux process is completed, the mixed solution in which the green light is immersed is filtered through a filter paper, for example, to filter a certain amount of the green light from the mixed solution, and the filtered green light is collected by mixing ethanol and deionized water. The process of secondary immersion in the mixed solvent thus obtained is carried out (step S8). Of course, even in this case, ethanol and deionized water constituting the mixed solvent are mixed at a ratio of 60:40, and the mixed solvent maintains a volume of 4 ml per 1 g of the green light.

Subsequently, when the above-described secondary immersion process is completed, the mixed solution in which the green light is immersed is secondary refluxed for 3 hours, for example, to extract a predetermined amount of the green light (Step S9).

When the secondary reflux process is completed, the mixed solution in which the green light is immersed is filtered through, for example, filter paper, and a certain amount of the green light is filtered out from the mixed solution, and then the filtered green light is collected in a size of, for example, 1000 g / m 2. The centrifugation process for 3 minutes to 6 minutes (step S10). After this series of centrifugation, the solid state extract is extracted with a certain amount of supernatant.

Thereafter, the supernatant extracted by the above centrifugation process is introduced into a condenser and a series of condensation processes are performed to remove all the solvent contained in the supernatant. Powdered green light extract is collected (steps S11 and S12). As described above, in the present invention, by using a mixed solution other than the organic solvent as the extract of the green light, the threat of skin damage due to the influence of the organic solvent can be eliminated, and the antioxidant activity of the green light is reduced through a series of heating processes. By reinforcing, the final finished product can be induced to have an effective function to neutralize the effects of free radicals that have been found to be a major cause of aging.

Thereafter, the preceding powdery green light extract is mixed with other substances, such as cosmetic raw materials, in an appropriate ratio, thereby completing the manufacture of a cosmetic having a certain level or more of quality.

On the other hand, the applicant proceeded the experiment described later to prove that the green light obtained through the practice of the present invention has excellent antioxidant activity compared to the green light obtained in the natural state.

First, the present applicant is "natural green light stem (A)", "a green light stem (B) which has undergone a heating process at 100 ° C based on the technical contents of the present invention", "natural green light root (C)" , "Mugunghwa root (D), which has undergone a heating process at 100 ° C based on the technical details of the present invention", respectively, was collected, and then pulverized, thereby preparing any sample as shown in Table 1.

Sample name A B C D weight 1.0215 g 1.0149 g 1.0040 g 1.0121 g

Subsequently, the Applicant immersed each sample A, B, C, D in 10 ml of methanol solution, respectively, and refluxed the individual methanol solution in which each sample was immersed for 30 minutes, thereby allowing four types of uncured stock solution. The four types of green light were filtered, and a series of secondary green light extracts were filtered, and the secondary green light extracts were condensed through a condenser to obtain four types of A, B, C, and D. Extracts were taken.

Subsequently, the Applicant uses the four types of green light extracts obtained as described above, and adds a mixed solution of ethanol and deionized water, for example, in a ratio of 60:40, in different amounts, and each of the green light extracts is represented in <Table 2>. As shown in Figure 6, six different concentrations of experimental solutions were prepared. Inhibition shown in Table 2 is a value commonly used to indicate the magnitude of the antioxidant activity. The larger the activation value, the greater the antioxidant activity.

Subsequently, the Applicant administered the same amount of DPPH (1,1-diphenyl-2-picrylhydrazyl) solution in each sample, and measured the antioxidant activity of each sample.

As a result of this measurement, each sample, for example, Samples A and B taken from the rose of sharon, was "24.18> 3.80 under the same concentration conditions, for example, concentration of 0.3125 (mg / ml), as shown in Table 2 "B> A" in order of antioxidant activity, "C> D from the roots of the green leaves," 4.35> 3.26 "in the order of antioxidant activity, that is," D> C "antioxidant activity The order is shown.

Sample Type Concentration (mg / ml) Inhibition (%) A 0.3125 3.80 0.625 10.33 1.25 23.91 2.5 36.96 5 63.59 10 91.85 B 0.3125 24.18 0.625 19.29 1.25 44.84 2.5 76.36 5 89.13 10 95.65 C 0.3125 3.26 0.625 2.99 1.25 14.13 2.5 23.91 5 39.13 10 76.90 D 0.3125 4.35 0.625 9.78 1.25 19.02 2.5 50.00 5 73.91 10 84.24

In addition, at different concentration conditions, such as 0.625 (mg / ml), samples A and B exhibited an antioxidant activity sequence of "19.29> 10.33", that is, an antioxidant activity sequence of "B> A". C and D showed an antioxidant activity sequence of "9.78> 2.99", that is, an antioxidant activity sequence of "D> C".

In addition, at different concentration conditions, such as 1.25 (mg / ml), samples A and B exhibited an antioxidant activity sequence of "44.84> 23.91", that is, an antioxidant activity sequence of "B> A". C and D showed an antioxidant activity sequence of "19.02> 14.13", that is, an antioxidant activity sequence of "D> C".

In addition, at different concentration conditions, such as 2.5 (mg / ml), Samples A and B showed an antioxidant activity sequence of "76.36> 36.96", that is, an antioxidant activity sequence of "B> A". C, D showed an antioxidant activity sequence of "50.00> 23.91", that is, an antioxidant activity sequence of "D> C".

In addition, at other concentration conditions, such as 5 (mg / ml), samples A and B exhibited an antioxidant activity sequence of "89.13> 63.59", that is, an antioxidant activity sequence of "B> A". C and D showed an antioxidant activity sequence of "73.91> 39.13", that is, an antioxidant activity sequence of "D> C".

In addition, at different concentration conditions, such as 10 (mg / ml), samples A and B exhibited an antioxidant activity sequence of "95.65> 91.85", that is, an antioxidant activity sequence of "B> A". C and D showed an antioxidant activity sequence of "84.24> 76.90", that is, an antioxidant activity sequence of "D> C".

Based on the experimental results of the present applicant, the present inventors displayed the graph as shown in FIG. 2, and the applicant showed "100 degreeC based on the technical content of the present invention, compared with the natural state of the green light stem (A)". It was confirmed that the green rose stem (B), which has undergone the heating process, has much better antioxidant activity, and compared to the "natural green rose root (C)", based on the technical content of the present invention, heating at 100 ° C. It was confirmed that the green rose root (D) having undergone the process has much better antioxidant activity.

As described in detail above, in the method for extracting the green light source according to the present invention, a series of heating processes are performed on the green light collected by forced drying, and the temperature of about 95 ° C to 105 ° C is applied to the green light. Heat is applied, thereby promoting tissue breakdown of the green light, thereby inducing the production of phenolic substances. Since these phenolic substances are known to possess excellent antioxidant properties, when the present invention is achieved, the green light can have a strong antioxidant activity, and eventually, the final finished product is found to be a major cause of aging free. It is possible to have an effective function to neutralize the effects of radicals.

In addition, in the present invention, the green liquid is extracted using a mixed solvent other than the conventional organic solvent, such as a mixed solvent in which the ratio of ethanol and deionized water is 60:40. Since ethanol and deionized water are known to have almost no effect on the skin, when the present invention is achieved, the green light extract can easily escape from the influence of the organic solvent, thus retaining excellent skin adaptability, The final finished product does not cause any problems such as damaging the skin unlike the conventional.

This invention shows the overall useful effect in a variety of products using green light.

And while certain embodiments of the invention have been described and illustrated, it will be apparent that the invention may be embodied in various modifications by those skilled in the art.

Such modified embodiments should not be understood individually from the technical spirit or point of view of the present invention and such modified embodiments should fall within the scope of the appended claims of the present invention.

Claims (4)

A first step of forcibly drying the rose of sharon collected from a part of the rose of sharon, and heating it at a temperature of 95 ° C to 105 ° C; A second step of forcibly drying the green light collected after the heating process and grinding the green light collected after the second forced drying; A third step of first immersing the pulverized green light collected in the grinding process in a mixed solvent of ethanol and deionized water in a ratio of 60:40, and then refluxing the first step; The mixed solvent having completed the first reflux is filtered through a series of filtration processes to select the green light from the mixed solvent, and the second green liquid is immersed in the mixed solvent for the second reflux. A fourth step; Filtering the mixed solvent having the secondary reflux through a series of filtration processes, selecting the green light from the mixed solvent, and centrifuging the selected green light from the mixed light for 3 minutes to 6 minutes to extract the supernatant. 5 steps; And a sixth step of condensing the supernatant and collecting a final extract of Mugunghwa. The method of claim 1, wherein the green light extract of the first step is the green light root extract or the green light stem extract. The method of claim 1, wherein the heating step of the first step is a method for extracting the green light raw material, characterized in that proceed for 22 to 26 hours by steam. The method of claim 1, wherein the mixed solvent of the third and fourth steps maintains a volume of 4 ml per gram of the green light.