KR20010103256A - Production method of tea including fermented grain with monascus sp. - Google Patents
Production method of tea including fermented grain with monascus sp. Download PDFInfo
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- KR20010103256A KR20010103256A KR1020000024564A KR20000024564A KR20010103256A KR 20010103256 A KR20010103256 A KR 20010103256A KR 1020000024564 A KR1020000024564 A KR 1020000024564A KR 20000024564 A KR20000024564 A KR 20000024564A KR 20010103256 A KR20010103256 A KR 20010103256A
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- tea
- green tea
- monascus
- brown rice
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- 238000004519 manufacturing process Methods 0.000 title claims description 15
- 241001095209 Monascus sp. (in: Fungi) Species 0.000 title 1
- 241001122767 Theaceae Species 0.000 title 1
- 244000269722 Thea sinensis Species 0.000 claims abstract description 51
- 235000013616 tea Nutrition 0.000 claims abstract description 34
- 235000009569 green tea Nutrition 0.000 claims abstract description 25
- 241000228347 Monascus <ascomycete fungus> Species 0.000 claims abstract description 24
- 235000021329 brown rice Nutrition 0.000 claims abstract description 20
- 240000006079 Schisandra chinensis Species 0.000 claims abstract description 8
- 235000008422 Schisandra chinensis Nutrition 0.000 claims abstract description 8
- 235000011511 Diospyros Nutrition 0.000 claims abstract description 6
- 244000236655 Diospyros kaki Species 0.000 claims abstract description 6
- 240000000249 Morus alba Species 0.000 claims abstract description 6
- 235000008708 Morus alba Nutrition 0.000 claims abstract description 6
- 244000126002 Ziziphus vulgaris Species 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 3
- 241000031003 Monascus ruber Species 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 238000001816 cooling Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 241000237502 Ostreidae Species 0.000 claims 2
- 235000020636 oyster Nutrition 0.000 claims 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 18
- 241000233866 Fungi Species 0.000 abstract description 10
- 235000012000 cholesterol Nutrition 0.000 abstract description 9
- 238000000855 fermentation Methods 0.000 abstract description 7
- 230000004151 fermentation Effects 0.000 abstract description 7
- 102000004190 Enzymes Human genes 0.000 abstract description 4
- 108090000790 Enzymes Proteins 0.000 abstract description 4
- 102000007330 LDL Lipoproteins Human genes 0.000 abstract description 4
- 108010007622 LDL Lipoproteins Proteins 0.000 abstract description 4
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 230000037361 pathway Effects 0.000 abstract description 4
- 229940026314 red yeast rice Drugs 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000003786 synthesis reaction Methods 0.000 abstract description 4
- 230000036772 blood pressure Effects 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract description 2
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 2
- 230000017531 blood circulation Effects 0.000 abstract description 2
- 235000013339 cereals Nutrition 0.000 abstract description 2
- 230000029087 digestion Effects 0.000 abstract description 2
- 230000006806 disease prevention Effects 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 239000012467 final product Substances 0.000 abstract description 2
- 229940124595 oriental medicine Drugs 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract description 2
- 235000009566 rice Nutrition 0.000 abstract description 2
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 150000004676 glycans Chemical class 0.000 abstract 1
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 abstract 1
- 229920001282 polysaccharide Polymers 0.000 abstract 1
- 239000005017 polysaccharide Substances 0.000 abstract 1
- 239000002994 raw material Substances 0.000 description 8
- RYYVLZVUVIJVGH-UHFFFAOYSA-N caffeine Chemical compound CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 235000009852 Cucurbita pepo Nutrition 0.000 description 3
- 240000001980 Cucurbita pepo Species 0.000 description 3
- 235000015459 Lycium barbarum Nutrition 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 229930185723 monacolin Natural products 0.000 description 3
- 241000272814 Anser sp. Species 0.000 description 2
- LPHGQDQBBGAPDZ-UHFFFAOYSA-N Isocaffeine Natural products CN1C(=O)N(C)C(=O)C2=C1N(C)C=N2 LPHGQDQBBGAPDZ-UHFFFAOYSA-N 0.000 description 2
- 244000241838 Lycium barbarum Species 0.000 description 2
- 235000015468 Lycium chinense Nutrition 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 229960001948 caffeine Drugs 0.000 description 2
- VJEONQKOZGKCAK-UHFFFAOYSA-N caffeine Natural products CN1C(=O)N(C)C(=O)C2=C1C=CN2C VJEONQKOZGKCAK-UHFFFAOYSA-N 0.000 description 2
- 230000004087 circulation Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000018553 tannin Nutrition 0.000 description 2
- 229920001864 tannin Polymers 0.000 description 2
- 239000001648 tannin Substances 0.000 description 2
- 235000009024 Ceanothus sanguineus Nutrition 0.000 description 1
- 102000004286 Hydroxymethylglutaryl CoA Reductases Human genes 0.000 description 1
- 108090000895 Hydroxymethylglutaryl CoA Reductases Proteins 0.000 description 1
- 240000003553 Leptospermum scoparium Species 0.000 description 1
- 244000171263 Ribes grossularia Species 0.000 description 1
- 235000002357 Ribes grossularia Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000019225 fermented tea Nutrition 0.000 description 1
- 235000020542 functional tea Nutrition 0.000 description 1
- 230000007407 health benefit Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/08—Oxidation; Fermentation
- A23F3/10—Fermentation with addition of microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/12—Rolling or shredding tea leaves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
- A23V2250/214—Tea
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/10—Drying, dehydrating
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Tea And Coffee (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
모나스쿠스(monascus)속의 균류를 이용한 발효는 약 2000년 전 고대 중국에서부터 곡주를 제조하기 위한 종국을 만들기 위하여 이용되어져 왔으며 한방에서는 혈액순환의 촉진, 소화력증진 등 질병의 예방목적으로 이용되어져 왔다.Fermentation using fungi of the genus Monascus has been used since 2000 to make a final product for producing grain wine from ancient China. In oriental medicine, it has been used for the prevention of diseases such as promoting blood circulation and enhancing digestion.
모나스쿠스(monascus)속 균주를 이용하여 발효한 쌀을 일반적으로 붉은 효모 쌀(red yeast rice)이라 하며 1977년 일본의 엔도 박사에 의해 그 효능이 과학적으로 입증되면서 그 가치가 제고되고 있다. 붉은 효모 쌀(red yeast rice)에는 콜레스테롤 합성경로의 중요 효소인 HMG-COA reductase inhibitor로 작용하는 물질이 포함되어 있어 콜레스테롤, 특히 저밀도지질단백(LDL) 콜레스테롤의 체내 증가를 억제해준다. 또한 혈압강하의 효과도 있는 것으로 알려져있다.Rice fermented with the strain of Monascus is commonly called red yeast rice, and its value is being enhanced by the scientifically proven efficacy of Dr. Endo in 1977. Red yeast rice contains a substance that acts as an HMG-COA reductase inhibitor, an important enzyme in the cholesterol synthesis pathway, and inhibits the increase in cholesterol, especially low-density lipoprotein (LDL) cholesterol. It is also known to have an effect of lowering blood pressure.
본 발명의 목적은 현재 광범위하게 이용되고 있는 녹차, 구기자, 오미자, 대추차, 감잎차, 뽕잎차, 둥굴레차 등의 다류에 monascus속의 균주를 이용하여 발효시킨 현미를 일정량 첨가하거나 차의 주원료 일정 비율로 함께 발효하여 모나스쿠스(monascus)속 균주에 의해 발효과정에서 생성되는 기능성 물질을 그대로 차류로 활용함으로써 기능성이 부가된 다(茶)류를 개발하는 것이다.An object of the present invention is to add a certain amount of brown rice fermented using strains of the genus monascus to a variety of teas, such as green tea, gojija, schisandra chile, jujube tea, persimmon leaf tea, mulberry leaf tea, donggle tea, which are widely used at present, By fermenting together, the functional material produced during the fermentation by the strain of the genus Monascus is used as a tea to develop a polysaccharide added with functionality.
Description
본 발명은 모나스쿠스(monascus)속의 균주를 이용하여 순수 배양한 배양물을 이용한 발효 다류의 제조방법으로, 더욱 상세하게는 일반 녹차잎과 둥굴레, 구기자, 오미자, 감잎, 뽕잎, 대추 등을 이용하여 다류를 제조하는데 있어 이러한 다류의 원료를 단독으로, 혹은 현미를 일정비율로 혼합하여 순수 배양된 모나스쿠스(monascus)속 균류 특히 모나스쿠스 루버(monascus ruber)를 이용하여 순수 고체 발효하여 제품화하거나, 혹은 별도로 발효가 완료된 현미를 차 원료와 일정비율로 혼합하여 제품화하는 것을 특징으로 하는 발효 다류의 제조방법에 관한 것이다. 일반적으로 녹차는 어린차잎 이나 보드라운 잎줄기를 채취한 후 이를 찌거나 볶아서 사용하였고, 기호도를 높이기 위하여 현미를 볶거나 가공하여 일정 비율로 섞어 현미녹차로 사용하여 왔으며 그 외의 전통차도 비슷한 방법으로 가공되어 왔다. 그러나 종래의 차들은 단순한 소재의 다류에 현미를 부가하여 현미에 포함된 일부의 영양소나 기호성을 부과하는데 비해 모나스쿠스(monascus)속 균류에 의해 고체 발효를 할 경우 발효과정에서 생성되는 생리활성 물질에 의해 여러 가지의 기능성이 부가될 수 있으며 종래의 단순혼합에 의한 가공에서 보다 기능성이 가미된 다류의 개발이 가능하다.The present invention is a method for producing a fermented tea using pure culture cultured using strains of the genus Monascus, more specifically using the green tea leaves and round gooseberry, wolfberry, Schisandra chinensis, persimmon leaf, mulberry leaf, jujube, etc. In the manufacture of teas, the raw materials of these teas are mixed alone or brown rice is mixed at a certain ratio to produce them by pure solid fermentation using a fungus of the genus Monascus, particularly Monascus ruber. The present invention relates to a method for producing fermented green tea, characterized in that the fermentation is completed and commercialized by mixing the brown rice with a certain ratio. In general, green tea has been used to take young tea leaves or botanical leaf stems, and then steam or stir them. To increase the degree of preference, brown rice has been roasted or processed and mixed in a proportion to be used as brown rice green tea. come. Conventional teas, however, add brown rice to a variety of simple ingredients and impose some nutrients or palatability in brown rice, whereas solid fermented by the fungus of Monascus is a bioactive substance produced during fermentation. Various functionalities can be added by this, and the development of the multi-class which adds more functionality to the conventional simple mixing process is possible.
예로부터 다류 특히 녹차는 동양에서 주로 음용 되어왔다. 차(茶)라함은 차나무의 어린잎이나 보드라운 줄기를 따서 제조 가공한 것을 일컫는 것으로 녹차이외의 차는 대용차라 할 수 있다. 일반적으로 차에는 단백질, 무기질, 비타민, 식이섬유소등이 포함되어 있으며 그 이외에 카페인(caffeine), 탄닌(tannin)등이 포함되어 있어 맛과 향뿐만 아니라 여러 가지 건강에 유익한 성분들을 함유하고 있다. 현재 유통되고 있는 제품의 형태는 원료를 찌거나 볶아서 분쇄하여 포장하거나. 잘게 썰어 포장한 것이 유통되고 있다. 모나스쿠스(monascus)속의 균류를 이용한 발효는 약 2000년 전 고대 중국에서부터 곡주를 제조하기 위한 종국을 만들기 위하여 이용되어져 왔으며 한방에서는 혈액순환의 촉진, 소화력증진 등 질병의 예방목적으로 이용되어져 왔다.Since ancient times, tea, especially green tea, has been mainly consumed in the Orient. Tea (茶) refers to the manufacture and processing of young leaves or boards stems of tea trees, tea other than green tea can be referred to as a substitute. In general, tea contains protein, minerals, vitamins, dietary fiber, etc. In addition, caffeine (caffeine), tannin (tannin), etc. are included not only taste and aroma but also various health benefits. The type of products currently in circulation can be packaged by steaming or roasting the raw materials. Finely sliced packages are in circulation. Fermentation using fungi of the genus Monascus has been used since 2000 to make a final product for producing grain wine from ancient China. In oriental medicine, it has been used for the prevention of diseases such as promoting blood circulation and enhancing digestion.
모나스쿠스(monascus)속 균주를 이용하여 발효한 쌀을 일반적으로 붉은 효모 쌀(red yeast rice)라하며 1977년 일본의 엔도 박사에 의해 그 효능이 과학적으로 입증되면서 그 가치가 제고되고 있다. red yeast rice에는 콜레스테롤 합성경로의 중요 효소인 HMG-COA reductase 저해제로 작용하는 물질이 포함되어 있어 콜레스테롤, 특히 저밀도지질단백(LDL) 콜레스테롤의 체내 증가를 억제해 주며 또한 혈압강하의 효과가 있는 것으로 알려져있다.Rice fermented using the strain of Monascus is commonly referred to as red yeast rice, and its value is being enhanced by scientifically proved by Dr. Endo in 1977. Red yeast rice contains a substance that acts as an inhibitor of HMG-COA reductase, an important enzyme in the cholesterol synthesis pathway, which inhibits the increase of cholesterol, especially low-density lipoprotein (LDL) cholesterol, and is known to be effective in lowering blood pressure. have.
본 발명은 모나스쿠스(monascus)속의 균류, 특히 모나스쿠스 루버(monascus ruber) 및 모나스쿠스 풀푸레우스(monascus purpreus)를 액체배지에서 순수배양하고 배양된 균사체를 전 처리된 차 원료, 혹은 현미와 일정비율로 혼합한 차 원료에 접종하고 이를 발효하거나 현미만을 발효한 후 이를 기존의 차 원료인 녹차, 둥굴레, 구기자, 오미자, 감잎, 뽕잎 등과 일정비율로 혼합하여 기존의 차에 부가된 기능을 갖는 차를 제조하는 것을 목적으로 한다. 차의 원료에 순수배양된 모나스쿠스(monascus)속 균류의 균사를 직접 접종하여 배양 발효한 경우 균사의 성장과정에서 생성 분비되는 여러 가지 유용한 생리활성물질, 특히 콜레스테롤 합성경로의 중요 효소인HMG-COA reductase의 저해 물질인 monacolin등을 다량 함유한 다류의 제조가 가능하므로 의약품 혹은 기능성 식품과는 달리 쉽게 상시 복용이 가능하고 부작용이 없으므로 실생활에 직접 접목이 가능하여 건강을 지키는데 유용하다.The present invention is a fungus of the genus Monascus, in particular, monascus ruber (monascus ruber) and Monascus purpurus (monascus purpreus) in pure culture in a liquid medium and cultured mycelium pretreated tea material, or brown rice and certain It is inoculated into the tea ingredients mixed at a ratio and fermented or only brown rice fermented, and then mixed with existing tea ingredients such as green tea, round gourd, wolfberry, schisandra, persimmon leaf, mulberry leaf in a certain ratio, and has a function added to existing tea For the purpose of manufacturing. Cultured fermented by directly inoculating mycelium of Monascus fungus purely cultured on tea raw materials, HMG-COA, an important enzyme of cholesterol synthesis pathway, especially useful bioactive substances produced during mycelial growth It is possible to manufacture a variety of products containing monacolin, a inhibitor of reductase, so it is easy to take it at any time unlike pharmaceuticals or functional foods.
본 발명은 모나스쿠스(monascus)속의 균류를 이용하여 차의 주원료를 고체 발효시키고 이를 건조한 다음 균을 불활화 시킨 후 건조, 혼합, 포장하는 것으로 좀더 상세히 설명하자면 무균조건에서 순수배양된 모나스쿠스(monascus)속 균류의 균사체를 현미 혹은 차의 원료로 이용되는 녹차잎, 둥굴레, 구기자, 오미자, 뽕잎, 감나무잎 등을 단독, 혹은 각각 일정비율로 혼합하여 멸균한 후 접종하고 25℃∼35℃에서 4∼25일간 배양한 후 건조하고 이를 다시 불활화하여 분쇄, 혼합, 포장하여 제품화 하는 것으로 일반적인 차의 기능에 부가적으로 monascus속의 균류에서 생성되는 2차 대사산물인 monacolin등이 함유된 다류의 제조가 가능해 지므로 혈압강하 및 콜레스테롤저하 등의 기능을 포함하는 기능성 다류의 제조가 가능해진다.The present invention is a solid fermentation of the main raw material of tea using a fungus of the genus Monascus, and then dried, mixed and packaged after inactivating the bacteria and then drying, mixing, and packaging the purely cultivated monascus (monascus) under aseptic conditions. The mycelia of the genus fungus are sterilized by mixing green tea leaves, round goose, gojija, schisandra chinensis, mulberry leaves, and persimmon leaves, which are used as raw materials of brown rice or tea, alone or in fixed proportions, and then inoculated and inoculated at 25 ℃ ~ 35 ℃. After culturing for ~ 25 days, dried, inactivated again, crushed, mixed, packaged and commercialized. In addition to the general tea function, the production of teas containing monacolin, a secondary metabolite produced by fungi of monascus, Since it becomes possible, it becomes possible to manufacture functional teas including functions, such as a blood pressure drop and a cholesterol lowering.
모나스쿠스(monascus)속 균류의 고체배양을 통한 발효녹차 및 전통차의 제조방법을 구체적으로 설명하면 아래 실시 예와 같다.The manufacturing method of the fermented green tea and the traditional tea through the solid culture of the fungus of the genus Monascus is as follows.
실시예 1.Example 1.
녹차잎, 구기자, 대추, 둥굴레, 오미자 등을 각각 20g씩 배양용기에 넣고 멸균기에서 121℃, 1.2기압에서 최소 20분이상 멸균한 후 상온까지 냉각하고 여기에 순수 액체 감자배지에서 배양된 모나스쿠스 루버(monascus ruber) 균사체를 5㎖ 접종하고 20℃∼30℃에서 4∼25일간 배양하여 45℃이하에서 건조하고 90℃에서 20분간 불활화 시킨 후 포장하였다.20g each of green tea leaves, gojija, jujube, round goose, and schisandra chinensis are placed in a culture vessel and sterilized at 121 ° C and 1.2 at least for 20 minutes in a sterilizer, cooled to room temperature, and cultivated in pure liquid potato medium. (monascus ruber) mycelia were inoculated in 5ml, incubated for 4-25 days at 20 ℃ ~ 30 ℃, dried below 45 ℃, inactivated at 90 ℃ 20 minutes and then packaged.
실시예 2.Example 2.
녹차잎과 현미를 각각 상온에서 0.5∼5시간, 4∼12시간 냉침한 후 중량대비 녹차잎 30∼95%, 현미 5∼70%가 되도록 배양용기에 넣고 실시예 1.과 같이 멸균한 후 monascus ruber 균사체를 접종하고 배양한 후 건조 포장하였다.After cooling green tea leaves and brown rice at room temperature for 0.5 to 5 hours and 4 to 12 hours, put them in a culture vessel so that the green tea leaves are 30 to 95% and brown rice 5 to 70% by weight, and sterilized as in Example 1. monascus Rub mycelium was inoculated and incubated and then dried and packaged.
실시예 3.Example 3.
구기자, 대추, 둥굴레, 오미자를 0.5∼5시간 상온에서 냉침한 후 각각 20g씩 배양용기에 넣고 실시예 1.과 같은 방법으로 멸균, 접종, 배양, 건조, 포장하였다.Gojija, jujube, round gourd, Schisandra chinensis were chilled at room temperature for 0.5 to 5 hours, and then placed in a culture vessel of 20g each, sterilization, inoculation, incubation, drying, packaging in the same manner as in Example 1.
실시예 4.Example 4.
실시예 1., 2., 3.의 접종 균종을 모나스쿠스 풀푸레우스(monascus purpreus) 순수배양 균사체로 대체하여 실시하였다.Inoculation strains of Examples 1., 2., and 3. were replaced with Monascus purpreus pure culture mycelia.
실시예 5.Example 5.
실시예 1., 2., 4.에서와 같이 배양 발효된 녹차 및 녹차와 현미혼합물 각각 10∼90%와 발효하지 않은 녹차잎 10∼90%를 혼합하여 포장하였다.As in Examples 1, 2, and 4, 10 to 90% of each of the cultured fermented green tea and green tea and brown rice mixture and 10 to 90% of the unfermented green tea leaves were mixed and packaged.
실시예 6.Example 6.
현미를 1∼10시간 냉침한 후 순수배양된 monascus ruber 균사체를 접종한 후 이를 4∼20일간 배양한 후 건조하고 불활화 시킨 후 분쇄하여 녹차잎 건조물 중량에 10∼70% 중량비가 되게 혼합하여 포장하였다.After soaking brown rice for 1-10 hours, inoculated with pure cultured monascus ruber mycelium, incubated for 4-20 days, dried, inactivated and ground to mix to dry tea leaf weight in a 10-70% weight ratio. It was.
본 발명은 monascus속의 균류 특히 monascus ruber 및 monascus purpreus를 순수배양하고 배양된 균사체를 전 처리된 차원료, 혹은 현미와 일정비율로 혼합한 차 원료에 접종하고 이를 발효하거나 현미만을 발효한 후 이를 기존의 차원료인 녹차, 둥굴레, 감잎 뽕잎, 구기자, 오미자 등과 일정비율로 혼합하여 기존의 차에 부가된 기능을 갖는 차를 제조하는 것을 목적으로 한다. 차의 원료에 순수배양된 모나스쿠스(monascus)속 균류의 균사를 직접 접종하여 배양 발효한 경우 균사의 성장과정에서 생성 분비되는 여러 가지 유용한 생리활성물질, 특히 콜레스테롤 합성경로의 중요 효소인 HMG-COA reductase의 저해 물질인 monacolin등을 함유한 다류의 제조가 가능하므로 다류의 부가가치를 창출할 수 있으며 의약품 혹은 기능성 식품과는 달리 상시 복용이 가능하고 부작용이 없으므로 실생활에 직접 접목이 가능하며 건강 증진에도 기여 할 수 있다.The present invention is inoculated with the fungus of the genus monascus, in particular monascus ruber and monascus purpreus and inoculated in the tea raw material mixed with the cultured mycelium in a pretreated raw material, or brown rice and a certain ratio and fermented or fermented only brown rice after It is an object to prepare a tea having a function added to the existing tea by mixing in a certain ratio with green tea, round gourd, persimmon leaf mulberry, gojija, Schisandra chinensis. When directly inoculated with the mycelium of Monascus fungus purely cultured on tea raw materials, HMG-COA is an important enzyme of cholesterol synthesis pathway. As it is possible to manufacture teas containing monacolin, an inhibitor of reductase, it is possible to create added value of teas. Unlike medicines or functional foods, it can be taken at all times and has no side effects. can do.
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Cited By (7)
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KR20010100061A (en) * | 2001-08-18 | 2001-11-14 | -- | Manufacturing method of mulberry extract and liquid beverage containing this |
WO2010062106A2 (en) * | 2008-11-25 | 2010-06-03 | (주)아모레퍼시픽 | Method for preparing fermented tea using bacillus genus strain |
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CN102726557A (en) * | 2011-04-02 | 2012-10-17 | 陕西功能食品工程中心有限公司 | Food possessing auxiliary hypolipidemic function |
KR101281572B1 (en) * | 2011-09-08 | 2013-07-03 | 명지대학교 산학협력단 | Preparation method of post-fermented tea using monascus pilosus and post-fermented tea prepared by the same |
KR101385176B1 (en) * | 2012-06-13 | 2014-04-14 | 명지대학교 산학협력단 | Mulberry leaves tea fetmented by genus monascus and method thereof |
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Cited By (9)
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KR20010100061A (en) * | 2001-08-18 | 2001-11-14 | -- | Manufacturing method of mulberry extract and liquid beverage containing this |
WO2010062106A2 (en) * | 2008-11-25 | 2010-06-03 | (주)아모레퍼시픽 | Method for preparing fermented tea using bacillus genus strain |
WO2010062106A3 (en) * | 2008-11-25 | 2010-07-29 | (주)아모레퍼시픽 | Method for preparing fermented tea using bacillus genus strain |
US8685475B2 (en) | 2008-11-25 | 2014-04-01 | Amorepacific Corporation | Method for manufacturing fermented tea using Bacillus SP. strains |
CN101744069A (en) * | 2010-01-29 | 2010-06-23 | 青岛万里江茶业有限公司 | Compound wild jujube tea |
CN102726557A (en) * | 2011-04-02 | 2012-10-17 | 陕西功能食品工程中心有限公司 | Food possessing auxiliary hypolipidemic function |
KR101281572B1 (en) * | 2011-09-08 | 2013-07-03 | 명지대학교 산학협력단 | Preparation method of post-fermented tea using monascus pilosus and post-fermented tea prepared by the same |
KR101385176B1 (en) * | 2012-06-13 | 2014-04-14 | 명지대학교 산학협력단 | Mulberry leaves tea fetmented by genus monascus and method thereof |
CN103859083A (en) * | 2014-04-01 | 2014-06-18 | 陕西汇源健康食品有限公司 | Cordyceps militaris Fuzhuan tea and production process thereof |
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