KR20010098182A - Method for Cultivating Mushroom Using Spoiled Milk - Google Patents

Abstract

The present invention relates to a method for culturing mushroom mycelium using mutated milk, comprising: diluting the mutated milk with 2-3 times of water and then adding a 1-5 weight carbon source to form a medium; Sterilizing the medium at 120-130 ° C. for 15 minutes to 2 hours; And incubating the medium by inoculating the mushroom seed after cooling the medium to room temperature.

Description

Method for Cultivating Mushroom Mycelium Using Decayed Milk {Method for Cultivating Mushroom Using Spoiled Milk}

The present invention relates to a method for culturing mushroom mycelium using mutated milk, and more particularly, to a method for maximizing the wastewater treatment effect and industrial utilization value by culturing the mushroom mycelium in a medium using mutated milk that is disposed of at high cost. will be.

Mushrooms are botanical microorganisms belonging to basidiomycetes or asymptomatic fungi, which are inhabited by more than 15,000 mushrooms worldwide, and about 50 are estimated to be edible. In Korea, about 230 kinds of edible or medicinal mushrooms have been reported. Among the mushrooms that are widely used for edible and medicinal mushrooms, the mushrooms that are artificially cultivated include shiitake ( Lentinula edodes ), pleurotus spp. And Flammulina velutipes. ), Ganoderma lucidum , Agaricus spp. And Paecilomyces japonica .

In order to artificially cultivate mushrooms, spawns, which are secondary mycelia of seeds, are used as seeds. The spawn used in mushroom cultivation should be easily decomposed by mushroom mycelium, so that the mycelial growth rate is fast and its adaptability to cultivation medium should be excellent. Most of the spawns currently in use were developed at the early stages of artificial cultivation, using solid media such as wood (stock), sawdust, rice straw, waste noodles, cotton husks (crustacean shells) and their mixed media. For example, raw wood (stock) spawns are removed from wood where mushroom mycelia are widespread and plugged into logs. As a spawn commonly used, various nutrients are added to sawdust and used as mushroom spawn.

With the recent rapid development of biotechnology, many mushrooms have attracted attention as materials for functional food and drug development. Edible mushrooms are evaluated by the quality and content of the components involved in nutritional characteristics (primary function), preference characteristics (secondary function), and dynamic bioregulatory function (tertiary function), which are static characteristics before eating. do. That is, mushrooms are nutritious (primary function); Tasty, visually beautiful, and not only contribute greatly to appetite by the unique aroma (secondary function); It has been found to contain various effective ingredients that exhibit pharmacological effects such as antibacterial action, antiviral action, cardiovascular action, cholesterol lowering action, blood sugar strengthening action, blood pressure lowering action, antithrombotic action and anti-tumor action. function).

On the other hand, in recent years, due to the long period of incubation of the fruiting body of the mushroom is limited production and requires a lot of space, even when compared to the mushroom fruiting body, mushroom mycelium, which is not inferior in terms of nutrition and medicinal effect, that is, the use of the seed is increasing Therefore, a method of cultivating cluster mycelium in a liquid state has been attempted.

Milk is a highly nutritious food containing various nutrients such as protein, lactose, fat, minerals and vitamins. It is widely produced and consumed regardless of East and West. Milk consists of 87.5 moisture, 3,5 fat, 3.25 protein, 0.75 mineral and 4.75 lactose. However, since milk is easily deformed at room temperature due to its high nutritional value, its hygienic treatment process and sterilization process are strictly controlled, and it is contaminated with pathogenic microorganisms when harvesting raw milk, or it cannot be used for food due to the deterioration of processed milk. The treatment of milk is treated by wastewater via physical, chemical or biological treatment.

The causative bacteria of milk are lactic acid bacteria, Escherichia coli, enterococci, Staphylococcus aureus, Micrococus ( Micrococus ), etc. There are various forms, colors, smells, etc. appear depending on the type of bacteria. In general, mutated milk is biodegraded and then treated with a method of removing difficult-decomposable organic substances using advanced wastewater treatment. In other words, first, slaked lime or alum is added to the secondary treated water to precipitate and remove phosphorus, and then nitrogen is removed using ammonia degassing or microbial denitrification. Then, the activated carbon adsorption tower is passed through to remove pesticides, industrial waste, lignin species and toxins, odorous substances, and the like, and then desalted using electrodialysis or reverse osmosis, followed by chlorine treatment to obtain final treated water.

By the way, the conventional method of processing the tainted milk has a high cost due to various equipment or materials in relation to the various stages of the treatment process.

It is an object of the present invention to provide a method for efficiently cultivating mushroom mycelium using coniferous milk.

Still another object of the present invention is to provide a method for efficiently and economically using condensed milk by cultivating mushroom mycelium at high cost.

Method for culturing the mushroom mycelium of the present invention in order to achieve the above object is to prepare a medium by diluting the decayed milk with 2-3 times the water and then adding a 1-5 weight carbon source; Sterilizing the medium at 120-130 ° C. for 15 minutes to 2 hours; And incubating the medium by inoculating the mushroom seed after cooling the medium to room temperature.

In the method for culturing mushroom mycelium according to the present invention, the decayed milk may be obtained by using milk that requires wastewater treatment as processed milk that is contaminated or deteriorated when the crude milk is collected.

Since milk consists of 87.5 moisture, 3,5 protein, 3.25 minerals, 0.75 minerals and 4.75 milk, it is possible to adjust the appropriate carbon and nitrogen ratio as a medium for cultivating mushroom mycelium by diluting wild milk 2 to 3 times. have.

In the method for culturing mushroom mycelium according to the present invention, any carbon source can be used as long as it can provide carbon such as powder of all grains, glucose and sugar.

Mushroom spawns that can be used in the present invention are Agaricus brazei , Phellinus linteus , Cordyceeps Syensis , Shiitake ( Lentinula edodes ), Pleurotus spp., Pleurotus spp., Mushrooms ( Flammulina velutipes ) , Ganoderma lucidum , Mushroom ( Tricholoma matsutake ), Mushroom ( Agaricus spp.), Auricularia auricula , Wolfiporia cocos , Hypsizigus marmoreus , Leaf mushroom ( Grifola frodosa ) For example, the strain of the mushroom selected from the group consisting of a mulberry mushroom ( Arillaria spp.) And fingering mushrooms (aka cloud mushroom, Coriolus versicolor ).

In the method of culturing mushroom mycelium according to the present invention, the culturing step may adjust the optimum culture temperature and incubation time according to the type of mushroom, and inoculate the mushroom spawn with a medium temperature of about 25-30 ° C. It is preferable to incubate at -30 ° C for 7-10 days, at which time it can be carried out using a conventional incubator.

As a result of culturing the mushroom mycelium using the modified milk according to the present invention, it was confirmed that the amount of dry mycelium was significantly increased as compared with the case of culturing using a conventional general nutrient medium. In addition, the produced mycelium showed no harmful toxicity and can be used for health food and medicinal use.

Hereinafter, the present invention will be described in detail by examples. However, the Examples are only for illustrating the invention and the present invention is not limited by the following Examples.

Example 1. Ganoderma lucidum mushroom

The addition of 2 l of drinking water to the deterioration of milk 1 l and then was put here for flour 100g in luxury. The acidity of the solvent was adjusted to pH 4.5 and then put into a 3 l culture tank and sterilized for 20 minutes at 121 ℃. Ganoderma spawn was aseptically inoculated when warmed to 25 ° C. After incubating at 28 ° C. for 5 days, dry weight 10.4 g of mycelium was obtained.

Example 2 Situation Mushroom

500 ml of drinking water was added to 1.5 l of condensed milk, and 30 g of glucose was added thereto, adjusted to pH 6, and placed in a 3 l culture tank for sterilization at 121 ° C. for 20 minutes. When warmed to 23 ° C., the seed was inoculated aseptically. After incubating at 30 ° C. for 8 days, 16.5 g of dry weight was obtained.

Example 3. Spirit Mushroom

Was added to the drinking water 1 l 1 l and then the deterioration of milk, 50g of glucose was added to it. The pH was adjusted to 5.5 and then put into a 3 l culture tank and sterilized for 20 minutes at 121 ℃. When the temperature was lowered to 27 ℃ aseptic seed spawn. After 7 days of incubation at 27 ° C. and 150 rpm, 18 g of mycelia were dried.

Comparative Example 1: Ganoderma lucidum mushroom

ingredient content

6 weights of glucose

Yeast Extract 0.6 Weight

KH ₂ PO ₄ 0.05 Weight

(NH ₄ ) ₂ HPO ₄ 0.1 Weight

The above composition was sterilized by making a medium of pH 5 and sterilized, and then spawned with Ganoderma spawn when warmed to 25 ℃. After incubating at 28 ° C. for 5 days, 15.2 g of dry weight was obtained.

Comparative Example 2: Situation Mushroom

ingredient content

4 weights of glucose

Peptone 0.6 Weight

Beef Extract 0.6 Weight

MgSO ₄ 7H ₂ O 0.2 Weight

The above composition was sterilized by making a medium of pH 5 and sterilized, and then, when the temperature was lowered to 30 ° C. After incubation at 30 ° C. for 8 days, 10.1 g of dry weight was obtained.

Comparative Example 3: Spirit Mushroom

ingredient content

Glucose 8 weight

Yeast extract 2 weight

KH ₂ PO ₄ 0.2 Weight

MgSO _{4 7} H ₂ O 0.1 Weight

CaCl ₂ · 2H ₂ O 0.2 Weight

The above composition was sterilized by spawning young mushroom spawn when it was sterilized by making medium of pH 5 and then cooled to 27 ° C. After incubating at 27 ° C. for 7 days, 9.7 g of dry weight was obtained.

The amount of dry mycelium of Examples 1 to 3 and Comparative Examples 1 to 3 is shown in Table 1 below.

Table 1

Example mushroom mushroom dry weight (g / l ) Comparative Examples mushroom mycelium a dry (g / l) Ganoderma lucidum mushroom 10.4 15.2 Situation Mushroom 16.5 10.1 Spirit Mushroom 18.0 9.7

As described above, according to the present invention, the mushroom mycelium can be obtained in high yield compared to the case of culturing the mushroom mycelium in the conventional medium by appropriately processing the decayed milk to be discarded and then used for culturing the mushroom mycelium. Not only that, but there is also an environmental and economic advantage by using the modified milk without the need for disposal.

Claims (5)

A method of culturing mushroom mycelium using rot milk as a medium. (a) diluting the modified milk with 2-3 times the water and then adding 1-5 weight of carbon source to make the medium (b) sterilizing the medium at 120-130 ° C. for 15 minutes to 2 hours; And (C) the culture method of the mushroom mycelium, characterized in that the medium is cooled to room temperature and then inoculated by inoculating the mushroom spawn. The method of claim 2, Inoculating the mushroom spawn at a medium temperature of about 25-30 ℃ in step (c) and incubated for about 7-10 days at about 25 to 30 ℃. The method of claim 2, In step (a), the carbon source is a method for culturing mushroom mycelium, characterized in that selected from the group consisting of powder, glucose and sugar of all grains. The method of claim 2, The mushroom spawn is Agaricus brazei , Phellinuslinteus , Cordyceeps Syensis , Lentinula edodes , Pleurotus spp., Flammulina velutipes , Ganoderma lucidum, Ganoderma lucidum ), Matsutake mushroom ( Tricholoma matsutake), matsutake mushroom ( Agaricus spp.), Matsutake mushroom ( Auricularia auricula ), ghost of worms ( Wolfiporia cocos ), mushrooms ( Hypsizigus marmoreus ), mushrooms ( Grifola frodosa ), mulberry mushroom ( Armillaria spp. .) And fingering mushrooms ( Coriolus versicolor ) culturing method of mushroom mycelium, characterized in that the strain of the mushroom selected from the group consisting of.