KR20010036693A - Microorganism insect composition comprising BT - Google Patents

Abstract

PURPOSE: A microbial insecticide capable of controlling harmful insects such as diamondback moth, beet armyworm and common cabbage butterfly that exert an influence on an amount and quality of various crops and containing bacillus thuringiensis is provide, thereby rapid action like a chemical agrochemical and causing no environmental pollution and residual toxicity is exhibited. CONSTITUTION: This microbial insecticide is prepared by mixing 1 to 60% by weight of dried Bacillus thuringiensis powder, 1 to 40% by weight of an improving agent for drug action, 1 to 30% by weight of a surfactant, 0 to 10% by weight of a binding agent and the balance a bulking agent. The binding agent is selected from the group consisting of aqueous starch, dextrin, carboxymethylcellulose, sodium polyacrylate, polyvinyl alcohol, arabic gum, xantane gum or two or more mixtures.

Description

Microorganism insect composition comprising BT

The present invention relates to a microbial insecticide composition containing Bacillus thuringiensis (hereinafter referred to as 'bitty') that can effectively control lepidopteran pests that damage various crops. In more detail, the present invention is a potentiator for microbial insecticide composition containing bitty, which is a microorganism derived from soil that can effectively control various lepidopteran pests such as Chinese cabbage moth, parchment moth, Chinese cabbage butterfly which causes harm to various crops. By adding to manufacture the drug can not only increase the drug efficacy, but also relates to a bitty-containing microbial insecticide composition having a high fast-acting, such as chemical pesticides.

Since humans began to farm in earnest, pests have been the most direct damage to farming, and the damage caused by these pests has reduced crop yields, quality, and merchandise, which has been a major factor in reducing farmers' income.

Therefore, many studies have been conducted to effectively control such pests, and there may be a method of using chemical pesticides as part of the control method, but the chemical pesticides are toxic to killing, residual toxicity in soil and crops. In particular, recently, there may be various problems such as the discovery of a substance acting as an environmental hormone. In order to solve these problems, developed countries have been engaged in the development of environmentally friendly biopesticides since the 1980s.

In particular, these biopesticides are safe for the environment, have little toxicity to human beings, and generally use microorganisms already present in nature to minimize pesticide use and variations in natural ecosystems. There are advantages to it.

To date, more than 50 kinds of microorganisms for insect control have been put to practical use all over the world, and many of them have reached practical stages. Such microorganisms include fungi, bacteria and viruses, and these are commonly used as pest control agents such as mites, aphids, root-knot nematodes, scarabs, Chinese cabbage moths, beetroot moths and greenhouse dusts. It is a product that uses more than 90% of the world's biological pesticides since it was put to practical use in 1948 and is registered and used as a pesticide in Korea.

However, when the treatment of the bitty agent, the drug efficacy on the 1st to 2nd day after the treatment was less than 50%, compared with the drug efficacy expected by the use of chemical pesticides, there was a problem in that the rapid dropping significantly.

In order to overcome this problem, a method of using a drug enhancer has been developed, a method of using amino acids and amides as a drug enhancer (Salama et al., 1989), a method using an inorganic salt or a surfactant (Morris et al. , 1995).

However, the method of using amino acids and amides as drug enhancers has a problem in commercialization due to the high price of amino acids and amides and poor physical properties after preparation.

In addition, the method of using calcium salts such as calcium oxide (CaO), calcium hydroxide (Ca (OH) ₂ ), calcium carbonate (CaCO ₃ ), etc., as an inorganic salt-enhancing drug enhances the effect, but these substances are also formulated as After the physical properties were poor, there was a problem in commercialization, in addition, copper salts such as copper oxide (CuO), copper sulfate (CuSO ₄ ), magnesium salts such as magnesium chloride (MgCl ₂ ), magnesium sulfate (MgSO ₄ ) and sodium salts The back had a problem that the drug efficacy effect is insufficient.

In addition, there are methods using potassium carbonate (K ₂ CO ₃ ), potassium hydrogen carbonate (KHCO ₃ ) and potassium hydrogen phosphate (K ₂ HPO ₄ ) as potassium salts. There was a problem of controlling less than 50%.

Therefore, there is a need to develop a prescription having high physical properties and high physical properties, such as chemical pesticides against the target pests of existing VT agents by improving these problems.

It is an object of the present invention to provide a microbial insecticide composition that can control pests such as Chinese cabbage moth, green chestnut moth, Chinese cabbage butterfly, etc. affecting the yield and quality of various crops, and the initial medicinal efficacy of the target pests by adding a medicinal enhancer. It is to provide a non-containing microbial insecticide composition having high fast-acting, such as chemical pesticides.

Another object of the present invention is not only excellent physical properties of the product even when formulated as a hydrating agent, granulating or liquid hydrating agent by adding a drug enhancer to the Beating agent, but also the storage stability of Beati and the control effect on the target pests as before the formulation The present invention provides a BTC-containing microbial insecticide composition, which is maintained and stable even at room temperature for at least one year.

Beati-containing microbial insecticide composition according to the present invention for achieving the above object, 1 to 40% by weight of the drug enhancer, 1 to 30% by weight surfactant, 0 to 10% by weight in 1 to 60% by weight dry The binder and the extender as the residual amount are uniformly mixed and then ground.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Beati-containing microbial insecticide composition according to the present invention is characterized in that it consists of a mixture of 1 to 60% by weight of bitter dry powder of 1 to 40% by weight of a potentiator and other surfactants, binders and extenders, It is characterized by having a high fast-acting effect such as chemical pesticides by promoting the initial drug efficacy against the target pest.

The bitty-containing microbial insecticide composition may be formulated with a hydrated powder by pulverizing the composition according to an embodiment of the present invention, not only the physical properties of the preparation are excellent after the preparation but also the storage stability of the bitty and control against the target pests. The effect is maintained as before the formulation, and characterized in that it provides a non-microbial pesticide composition containing a microbial component stable for at least one year even when stored at room temperature.

The bitter dry powder is composed of resistant spores and toxins that play a direct role in Chinese cabbage, moth, cabbage moth, Chinese cabbage butterfly, etc., and the toxin produced by bitty is activated by strong alkaline digestion in the middle of the pest. Destroys the cells of the insecticide.

The bitty dry powder consists of spores of 1.0 * 10 ⁵ to 1.0 * 10 ⁹ spores / mg.

Such bitty dried powder is already known to those skilled in the art as a pesticide product license and the like and its production and usage are known, and also can be understood to be known enough to be commercially available.

The drug enhancer enters the middle of the pest along with the bite toxin protein to help the toxin protein to quickly convert to the form of the active toxin or to physically damage the middle barrier to enhance the action of the toxin as well as physiological in the body of the pest Potassium chloride (KCl), calcium chloride ?? (CaCl ₂ ), calcium carbonate (CaCO ₃ ), sodium carbonate (Na ₂ CO ₃ ) or mixtures of two or more of these substances act to induce changes to enhance the effect of toxins Inorganic salts selected from the group consisting of can be used.

The surfactant is an amphiphilic material having both hydrophilic and lipophilic molecular groups in a molecule, and is understood to have characteristics of excellent cleaning, dispersing, emulsifying, solubilizing, wetting, bactericidal, foaming and penetrating properties. It can be understood that the bite in the microbial insecticide composition according to the present invention functions to hydrate, suspend and disperse to effectively express the drug.

The surfactant may be a sodium salt of a sulfonate such as alkylbenzenesulfonate, alkylnaphthalenesulfonate, dialkylsulfosuccinate, ligninsulfonate, alkylnaphthalenesulfonate formalin condensate, polyoxyalkylene alkylphenylsulfonate or Sodium salts of sulfates such as calcium salts, alkyl sulfates, polyoxyalkylene alkyl sulfates, polyoxyalkylene alkylphenyl sulfates or sodium salts of succinates such as calcium salts, naphthalenesulfosuccinates, polyoxyalkylene succinates or Anionic surfactants such as calcium salts, ethoxylated alkyl ethers, polyoxyalkylene alkylphenyl polymers, and nonionic surfactants such as multiple alcohols may be used alone or in combination of two or more thereof, all of which are listed by way of example. It is common in the art that surfactants other than these may be used as these. Now with the knowledge it will be readily understood.

The binder serves to bind the drug enhancer, the extender, and the like, including the non-drying powder of the active ingredient, water-soluble starch, dextrin, carboxymethyl cellulose, sodium polyacrylate, polyvinyl alcohol, gum arabic or xanthan gum. These may be used alone or in combination of two or more thereof.

The extender is used in conjunction with the surfactant to adsorb and powder the surfactant and, together with the surfactant, the potentiator, the bitter dry powder and the toxin protein, functions as a material forming the particulate surface of the bitter composition, starch, Soybean meal, bran, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kaolin, pyrophyllite, white carbon and the like may be used alone or in combination of two or more thereof.

According to another embodiment of the present invention, 1 to 60% by weight of bitty dry powder, 1 to 40% by weight of a potentiator, 1 to 30% by weight of surfactant, 0 to 10% by weight of binder, and an extender as a balance After mixing, kneading, injection and drying through a granulator, it can be used as a granulating agent.

The bitter dry powder may be a dry powder used in the bitty-containing microbial insecticide composition according to an embodiment of the present invention, it can be understood to have the same function as the hydrating agent composition.

The surfactant serves to hydrate, suspend, disperse, and disintegrate the bitty-containing microbial insecticide composition granulating agent according to the present invention, and the same ones as the surfactants provided in the hydrating agent composition of the present invention may be used.

The binder and extender have the same function as in the hydrating agent composition of the present invention, and the same binder and extender as the hydrating agent composition may be used.

The BTC-containing microbial insecticide composition according to another embodiment of the present invention may contain 1 to 40% by weight of a potentiator, 1 to 30% by weight of surfactant, 1 to 10% by weight of 1 to 60% by weight of BT dry powder. 30% by weight of a 70% by weight pulverized portion obtained by wet pulverization of a mixture of water as an extender and a residual amount by mixing, 1 to 20% by weight of a binder, 1 to 20% by weight of an antifreeze agent, and water as a residual amount. It is made by dispersing the thickener uniformly and can be used as a liquid hydrating agent.

The bitter dry powder may be a dry powder used in the bitty-containing microbial insecticide composition according to an embodiment of the present invention, it can be understood to have the same function as the hydrating agent composition.

The surfactant serves to hydrate, suspend, and disperse the bitty-containing microbial insecticide composition liquid hydrating agent according to the present invention, and the same surfactants as those shown in the hydrating agent composition of the present invention may be used.

The binder and extender have the same function as in the hydrating agent composition of the present invention, and the same binder and extender as the hydrating agent composition may be used.

The cryoprotectant is for inhibiting the freezing of the entire composition by inhibiting the freezing of the water contained in the liquid crystallization agent of the bitty-containing microbial insecticide composition according to the present invention, the substance of glycols such as ethylene glycol, propylene glycol alone or two kinds It can be selected and used among the above mixture.

Hereinafter, preferred embodiments and comparative examples of the present invention will be described.

The following examples are intended to illustrate the invention and should not be understood as limiting the scope of the invention.

Example 1

40 kg of dried powder of Bacillus thuringiensis sbusp.aizawai is mixed evenly with 30 kg of potassium chloride, 12 kg of ethoxylated alkyl ether, 8 kg of tetramethyl-5-decyl-4,7-diol, and 10 kg of white carbon And pulverized to obtain a bitty-containing microbial insecticide composition for hydration according to the present invention. The obtained hydrating agent was used for strain stability and biotest.

Example 2

In the present invention, 40 kg of bitty dry powder was mixed with 15 kg of potassium chloride, 15 kg of sodium carbonate, 12 kg of ethoxylated alkyl ether, 8 kg of tetramethyl-5-decyl-4,7-diol, and 10 kg of white carbon. A bitty-containing microbial insecticide composition for hydration was obtained. The obtained hydrating agent was used for strain stability and biotest.

Example 3

30 kg of potassium chloride, 15 kg of ethoxylated alkyl ether, 5 kg of dextrin, and 10 kg of white carbon were uniformly mixed and kneaded in a 40 kg bitty dry powder, followed by injection into a particle diameter of 1 mm through a granulator to ventilate and dry. The dried granules were cut into lengths of 1 to 3 mm to obtain a bitty-containing microbial insecticide composition for granulating agent according to the present invention. The obtained granulating agent was used for strain stability and biotest.

Example 4

15 kg of potassium chloride, 15 kg of sodium carbonate, 15 kg of ethoxylated alkyl ether, 5 kg of dextrin, and 10 kg of white carbon were uniformly mixed and kneaded in a 40 kg bitty dry powder. . The dried granules were cut into lengths of 1 to 3 mm to obtain a bitty-containing microbial insecticide composition for granulating agent according to the present invention. The obtained granulating agent was used for strain stability and biotest.

Example 5

70 kg of 70 kg obtained by mixing and wet grinding 15 kg of potassium chloride, 5 kg of ethoxylated alkyl ether, 5 kg of tetramethyl-5-decyl-4,7-diol, 5 kg of white carbon, and 20 kg of water in 20 kg of bitty dry powder The grind portion was uniformly dispersed by uniformly mixing the 30 kg thickener obtained by mixing 10 kg of dextrin, 5 kg of ethylene glycol, and 15 kg of water to obtain a bitty-containing microbial insecticide composition for a liquid wetting agent according to the present invention. The obtained liquid hydrating agent was used for strain stability and biotest.

Example 6

20 kg bitty dry powder is mixed with 10 kg of potassium chloride, 5 kg of calcium carbonate, 5 kg of ethoxylated alkyl ether, 5 kg of tetramethyl-5-decyl-4,7-diol, 5 kg of white carbon, and 20 kg of water The 70 kg crushed portion obtained by mixing 10 kg of dextrin, 5 kg of ethylene glycol, and 15 kg of water was uniformly dispersed by uniformly dispersing the 30 kg thickened portion, thereby obtaining a bitty-containing microbial insecticide composition for liquid wetting agent according to the present invention. . The obtained liquid hydrating agent was used for strain stability and biotest.

Comparative Example 1

12 kg of ethoxylated alkyl ether, 8 kg of tetramethyl-5-decyl-4,7-diol, and 40 kg of white carbon were uniformly mixed and ground to 40 kg of bitty dry powder to obtain a microbial insecticide for hydration as a control. The obtained liquid hydrating agent was used for strain stability and biotest.

Comparative Example 2

15 kg of ethoxylated alkyl ether, 5 kg of dextrin, and 40 kg of white carbon were uniformly mixed and kneaded in 40 kg of bitty dry powder, and injected into a particle diameter of 1 mm through a granulator to be ventilated and dried. The dried granules were cut to a length of 1 to 3 mm to obtain a microbial insecticide composition for granulating agent as a control. The obtained granulating agent was used for strain stability and biotest.

Comparative Example 3

10 kg of a 70 kg mill obtained by mixing 20 kg of bitty dry powder with 5 kg of ethoxylated alkyl ether, 5 kg of tetramethyl-5-decyl-4,7-diol, 5 kg of white carbon, and 35 kg of water and wet grinding 30 kg thickener obtained by mixing dextrin, 5 kg of ethylene glycol, and 15 kg of water was uniformly mixed and uniformly dispersed to obtain a microbial insecticide composition for liquid hydration as a control. The obtained liquid hydrating agent was used for strain stability and biotest.

Comparative Example 4

In order to compare the medicinal effects of the BTC-containing microbial insecticide composition according to the present invention against the Chinese cabbage moth, the official control agent against the Chinese cabbage moth was Shuriside (Thricide) 16BIU hydrate.

Comparative Example 5

In order to compare the medicinal effects against the Chinese cabbage moth of the bitty-containing microbial insecticide composition according to the present invention was used Xentari 35000BIU granulifying agent, another official control against Chinese cabbage moth.

Experimental Example 1

Strain stability and potency enhancement effect before and after preparation

1 g of each of the compositions prepared according to the above Examples and Comparative Examples was quantitatively taken into a test tube containing sterile water, and then the titers before and after the preparation were examined according to the standard BTT titer assay. Shown in

Activity test results before and after preparation division Titer of pre-dose BTI (BIU / kg) Titer of Beati Post-Formulation (BIU / kg) Theory Test result Example 1 50 20 30 Example 2 50 20 29 Example 3 50 20 32 Example 4 50 20 31 Example 5 50 10 16 Example 6 50 10 14 Comparative Example 1 50 20 21 Comparative Example 2 50 20 20 Comparative Example 3 50 10 11

As shown in Table 1, the compositions of the comparative examples show a potency similar to the theoretical value, while the compositions of the examples according to the present invention show an enhancement effect in the titer after preparation, and thus the present invention is a microbial insecticide having insecticidal activity. By formulating for practical use, there was no problem in strain stability, and it could be confirmed that the BTC-containing microbial insecticide composition exhibiting the drug-promoting effect by the drug-promoting agent was confirmed.

Experimental Example 2

Investigate the stability of products over time

After putting the BTC-containing microbial insecticide composition according to the present invention prepared in each of the above in a sealed container, the sample is taken by the number of days while leaving in the incubator adjusted to 40 ℃ by a certain amount of the sample by the standard bitty titer assay Titers were investigated and the results are shown in Table 2 below.

Result of stability survey by days of product division Product titer (BIU / kg) 4 weeks later 8 weeks later 12 weeks later Example 1 30 30 29 Example 2 29 30 33 Example 3 32 32 30 Example 4 31 29 31 Example 5 15 15 14 Example 6 16 15 16

As shown in Table 2, when the bite-containing microbial insecticide compositions according to the present invention was subjected to a change in abuse over time for 4 weeks, 8 weeks, 12 weeks at 40 ℃, there was no significant difference in the titer of the microorganism, The composition of the present invention was confirmed to provide a bitty-containing microbial insecticide composition without problems in the stability of the microorganisms in the microbial insecticide composition even after long-term storage.

Experimental Example 3

Efficacy on Chinese cabbage moth indoors

In order to confirm the control effect on the Chinese cabbage moth, a test was performed indoors with the medicaments of the Examples and Comparative Examples. After the cabbage was formulated in each test pot, the composition prepared according to each example and the composition of the comparative examples were treated with leaves by diluting fold, and then inoculated with Chinese cabbage moth. Drug efficacy was determined on day 1, day 2, and day 4 after drug treatment, the results are shown in Table 3 below.

Drug test results for Chinese cabbage moth indoors division Dilution factor (fold) Chinese cabbage moth dictionary density Control effect (%) Day 1 Day 2 Day 4 Example 1 10,000 20 72.9 100.0 100.0 Example 2 10,000 20 68.8 95.7 99.9 Example 3 10,000 20 70.8 97.8 100.0 Example 4 10,000 20 66.7 95.7 99.3 Example 5 5,000 20 61.1 89.4 96.5 Example 6 5,000 20 63.1 87.7 97.4 Comparative Example 1 10,000 20 20.0 62.9 91.7 Comparative Example 2 10,000 20 32.1 67.6 88.3 Comparative Example 3 5,000 20 24.4 60.9 92.6 Comparative Example 4 10,000 20 20.0 61.5 93.7 Comparative Example 5 10,000 20 12.1 67.4 86.5 No treatment - 20 - -

As shown in Table 3, the bitty-containing microbial insecticide composition prepared according to the embodiments of the present invention compared to the pharmaceutical composition of the comparative examples and commercialized comparative examples without the addition of a potentiator according to the first and second investigations In the 4th day pharmacological investigation result showed a markedly high control effect compared to the drugs of the comparative examples, the BTC-containing microbial insecticide compositions according to the present invention is excellent in the control effect against Chinese cabbage moth by the drug enhancer It was confirmed that the present invention provides a composition that exhibits high fast-acting properties, such as chemical pesticides.

Experimental Example 4

Efficacy on Chinese cabbage moth outdoors

In order to confirm the control effect on the Chinese cabbage moth, the test was performed outdoors with the medicaments of the Examples and Comparative Examples. After the cabbage was planted in the field soil, the composition of the compositions and the comparative examples prepared according to the respective examples were treated with leaves according to the dilution factor and then inoculated with cabbage moths. Drug efficacy was determined on day 2, day 4, and day 7 after drug treatment, and the results are shown in Table 4 below.

Medicinal test results for Chinese cabbage moths outdoors division Dilution factor (fold) Chinese cabbage moth dictionary density Control effect (%) Day 2 Day 4 Day 7 Example 1 1,000 20 68.8 83.3 100.0 Example 2 1,000 20 66.5 81.5 100.0 Example 3 1,000 20 66.7 78.6 99.3 Example 4 1,000 20 56.3 80.1 100.0 Example 5 500 20 60.2 80.4 100.0 Example 6 500 20 54.3 78.2 99.1 Comparative Example 1 1,000 20 30.1 45.3 98.9 Comparative Example 2 1,000 20 24.1 46.1 97.6 Comparative Example 3 500 20 22.8 48.4 93.8 Comparative Example 4 1,000 20 20.1 50.1 96.1 Comparative Example 5 2,000 20 24.3 46.3 85.8 No treatment - 20 - -

As shown in Table 4, the bitty-containing microbial insecticide composition prepared according to the present invention was shown to be slightly less effective than the indoor test, the drug of Comparative Examples 1, 2, 3 without the addition of the drug enhancer even in the outdoor test And compared to the commercialized drugs of Comparative Examples 4, 5, 2, 4, 7 days of drug investigation results, markedly excellent drug efficacy by the drug enhancer according to the present invention is not only excellent control effect on Chinese cabbage moth But it could be confirmed that it provides a composition having a high fast-acting, such as chemical pesticides.

Experimental Example 5

Efficacy of pabam moth in outdoor test

The test was performed outdoors with the medicaments of the Examples and Comparative Examples to confirm the control effect on the beetroot moth. Chinese cabbage was formulated in the field soil, and the leaves of each of the composition drugs and the comparative examples prepared according to the examples were treated with each leaf by dilution and then inoculated with pabam moths. Drug efficacy was determined on day 3, day 5, and day 7 after drug treatment, and the results are shown in Table 5 below.

Pharmacokinetic test results for parka moths outdoors division Dilution factor (fold) Chinese cabbage moth dictionary density Control effect (%) Day 3 Day 5 Day 7 Example 1 1,000 20 56.1 76.9 95.4 Example 2 1,000 20 40.6 72.1 95.1 Example 3 1,000 20 55.3 74.3 93.4 Example 4 1,000 20 44.9 72.4 95.7 Example 5 500 20 53.5 73.2 92.1 Example 6 500 20 54.1 73.9 93.9 Comparative Example 1 1,000 20 22.2 57.1 89.9 Comparative Example 2 1,000 20 20.8 56.3 80.1 Comparative Example 3 500 20 18.6 55.2 81.2 Comparative Example 4 1,000 20 8.1 34.8 57.7 Comparative Example 5 1,000 20 24.2 65.1 90.6 No treatment - 20 - -

As shown in Table 5, the BTC-containing microbial insecticide compositions prepared according to the present invention showed an excellent control effect against pabam moths compared to the drugs of Comparative Examples 3, 5, 7 days It was confirmed that the drug enhancer according to the present invention not only significantly improves the efficacy but also provides a composition having high fast-acting effects such as chemical pesticides.

As a result of the synthesis of the above embodiments, the present invention is a microorganism in the product is stably preserved before, after and during the preparation, the drug enhancer significantly increases the initial control effect on the Chinese cabbage, moth, night chestnut moth Not only can provide a composition of high fast-acting composition like pesticides, but also provides a composition with superior drug efficacy in comparison with the pharmaceuticals of the comparative examples in the final control effect, and effectively and without the problems of environmental and residual toxicity like conventional chemical synthetic pesticides. It was confirmed that the Chinese cabbage moth and the like can be controlled.

Therefore, according to the present invention, by adding a drug-promoting agent to the existing BTC-containing preparations, it is possible not only to improve the efficacy of the BTT against lepidopteran pests such as Chinese cabbage moth, Pabam moth and Chinese cabbage butterfly, but also high chemical pesticides. It is effective in providing a high-efficiency insecticide composition that exhibits fast-acting effect and solves the problems of environmental pollution and residual toxicity that may be caused by the use of chemical pesticides.

In addition, according to the present invention, even when prepared by the addition of a medicament enhancer, hydrated, granulated or liquid hydrated, not only the physical properties of the product are excellent, but also the stability and control of pests are maintained as before the preparation, and stored at room temperature It also has the effect of providing a non-containing microbial insecticide composition in which the non-bite components remain stable for at least 1 year.

Although the present invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical scope of the present invention, and such modifications and modifications are within the scope of the appended claims.

Claims (9)

1 to 60% by weight of bitty dry powder, 1 to 40% by weight of drug enhancer, 1 to 30% by weight of surfactant, 0 to 10% by weight of binder, and the remaining amount of extender as uniformly mixed and ground Beati-containing microorganism insecticide composition which consists of. The method of claim 1, The bitty dried powder is composed of resistant spores and toxins, and Bacillus thuringiensis exhibiting insecticidal action by toxins, wherein the bitty-containing microorganism is characterized in that it consists of spores of 1.0 * 10 ⁵ to 1.0 * 10 ⁹ spores / mg. Insecticide Composition. The method of claim 1, The medicament enhancer is an inorganic salt selected from the group consisting of potassium chloride (KCl), calcium chloride ?? (CaCl ₂ ), calcium carbonate (CaCO ₃ ), sodium carbonate (Na ₂ CO ₃ ) or a mixture of two or more thereof. Beati-containing microbial insecticide composition. The method of claim 1, The surfactants are sodium salts or calcium of sulfonates such as alkylbenzenesulfonates, alkylnaphthalenesulfonates, dialkylsulfosuccinates, ligninsulfonates, alkylnaphthalenesulfonate formalin condensates, polyoxyalkylenealkylphenylsulfonates Sodium salts of calcium sulfates such as salts, alkyl sulfates, polyoxyalkylene alkyl sulfates, polyoxyalkylene alkylphenyl sulfates or sodium salts of calcium salts, succinates such as naphthalenesulfosuccinate, polyoxyalkylene succinate or calcium The bitty-containing microbial insecticide composition, characterized in that it is selected from the group consisting of anionic surfactants such as salts, ethoxylated alkyl ethers, polyoxyalkylene alkylphenyl polymers, nonionic surfactants such as polyalcohols or mixtures of two or more thereof. . The method of claim 1, And said binder is selected from the group consisting of water-soluble starch, dextrin, carboxymethylcellulose, sodium polyacrylate, polyvinyl alcohol, gum arabic, xanthan gum or mixtures of two or more thereof. The method of claim 1, The bitty-containing microorganism, characterized in that the extender is selected from the group consisting of starch, soybean meal, bran, granular fiber, yuan, diatomaceous earth, zeolite, bentonite, talc, kaolin, pyrophyllite, white carbon or a mixture of two or more thereof. Insecticide Composition. The method of claim 1, 1 to 60% by weight of bitty dry powder is uniformly mixed with 1 to 40% by weight of drug enhancer, 1 to 30% by weight of surfactant, 0 to 10% by weight of binder, and extender as the remaining amount, and then kneaded through a granulator Beati-containing microbial insecticide composition, characterized in that the injection and drying. The method of claim 1, 70% obtained by wet grinding a mixture of 1 to 60% by weight of bitty dry powder with 1 to 40% by weight of a potentiator, 1 to 30% by weight of surfactant, 1 to 10% by weight of extender, and the remaining amount of water Beatitic microorganism, characterized by uniformly mixing and dispersing 30% by weight of the thickening portion of the crushed portion, 1 to 20% by weight of the binder, 1 to 20% by weight of the freezing agent, water remaining as a balance Insecticide Composition. The method of claim 8, The bitty-containing microbial insecticide composition, characterized in that the cryoprotectant is selected from the group consisting of ethylene glycol, propylene glycol or a mixture thereof.