KR20000058441A - Method for preparing a low molecular weight polysaccharides and it's oligosaccharides - Google Patents

Abstract

PURPOSE: An aqueous low molecular polysaccharide and oligosaccharide thereof are provided which can be used in medicines, food-additive, cosmetic, natural pesticide, and etc.. CONSTITUTION: The low molecular polysaccharide and oligosaccharide thereof are produced by a process comprising the steps of: dispersing the polysaccharide such as pectin, chitin, chitosan, alginic acid, cellulose, and etc. in solvent such as pure water, and acetic acid; and adding a mixture of ozone/oxygen to the dispersed solution, wherein an amount of the ozone is 0.01-10 mole (based on the glucose amine of 1 mole).

Description

Method for preparing a low molecular weight polysaccharides and oligosaccharides thereofMethod for preparing a low molecular weight polysaccharides and it's oligosaccharides

The present invention relates to a method for producing low molecular weight polysaccharides and oligosaccharides thereof by dissolving polysaccharides in a solvent and blowing ozone into the solution. These water-soluble low molecular weight polysaccharides and oligosaccharides are substances that can be used in foods, seasonings, feed, fertilizers, nutritional supplements, cosmetics and pharmaceuticals.

Conventional methods for obtaining low molecular polysaccharides and oligosaccharides thereof have been carried out by enzymatic or acid decomposition methods of polysaccharides. However, the enzymatic digestion method has a disadvantage in that the location of cleavage and degradation of polysaccharides is limited due to the specificity of the enzyme, thereby limiting the types of degradation products that can be obtained. In addition, in the case of acid decomposition, since polysaccharides are decomposed in a strong acid solution such as concentrated hydrochloric acid, a large amount of monosaccharides are generated, and the conversion efficiency is low. Finally, a neutralization process is required, a desalting process is essential, and a pure low molecular weight It is difficult to obtain polysaccharides and oligosaccharides. In addition, even in the method for preparing low molecular weight polysaccharides by the present invention (application number 032474), pure low molecular weight polysaccharides can be obtained, but the manufacturing time is long.

The present invention relates to a method for producing low molecular weight polysaccharides and oligosaccharides thereof by efficiently blowing ozone into a solution in which polysaccharides are dissolved at a constant concentration in a suitable solvent such as distilled water.

The present invention relates to a method for preparing low molecular polysaccharides and oligosaccharides thereof. That is, low molecular polysaccharides formed by dissolving polysaccharides at a constant concentration in a suitable solvent such as distilled water or acetic acid solution, and decomposing by blowing ozone into the solution. A method for efficiently producing oligosaccharides thereof.

Polysaccharides used in the present invention include chitin, chitosan, alginic acid, pectin, inulin, cellulose, gum, starch, glycogen, heparin, hyaluronic acid, polygalactoseamine and derivatives thereof. The concentration of the polysaccharide solution used in the production may be 0.5 to 20% by weight.

The ozone used in the present invention uses a mixture of ozone / oxygen and the amount of ozone may use 0.01 to 10 times mole of the unit mole of glucoseamine, and preferably 0.1 to 2 times mole.

In the present invention, for example, 5 parts by weight of chitosan is dispersed in 100-200 parts by weight of a 5% acetic acid solution and stirred at 5 to 50 ° C. to prepare a low molecular weight polysaccharide and an oligosaccharide thereof. A low molecular weight chitosan and its oligosaccharides were prepared by reacting the solution with an ozone / oxygen mixture at a rate of 2.4 to 5.4 L / h at 30 to 3 hours at 20 to 65 ° C. Other polysaccharides were also prepared by means of fraud.

Hereinafter, the present invention will be described in detail with reference to Examples.

Example 1

50 g of chitosan (molecular weight: 180000) was dissolved in 1000 g of 5% acetic acid aqueous solution, and the solution was reacted with an ozone / oxygen gas at 60 ° C. for 3 hours at a rate of 5.4 L / h to prepare a chitosan decomposition product. And the decomposition products were used as a solution or spray-drying as it was decomposed.

Example 2

The same procedure as in Example 1 was carried out except that the reaction was carried out at 20 ° C, 40 ° C, and 80 ° C.

Example 3

Sodium alginate (molecular weight: 270000) was carried out in the same manner as in Example 1, except that 5 parts by weight were dissolved in 100 parts by weight of distilled water.

Example 4

The average molecular weight of the degradation product and the oligosaccharide obtained by the method of Example 1-3 was analyzed by GPC. Shodex OHpak SB-801 + SB-802 + SB-803 (7.5 mm ID x 300 mm L) was used, the eluate was used for 0.1 M acetic acid buffer, and the flow rate was adjusted to 0.8 ml / min. The results are shown in Table 1.

Example Temperature Molecular weight according to decomposition time (Mn) 5 minutes 10 minutes 15 minutes 20 minutes 30 minutes 60 minutes 120ven One 20 125000 105000 87000 73000 51000 27800 13000 40 79000 50000 39000 31000 21000 9400 5900 60 49000 28800 18000 12000 9900 4000 3500 80 41000 22000 13000 9800 6700 3500 1800 3 20 197000 122000 102000 85000 57000 33000 23000 40 123000 114000 91000 75000 53000 24000 9500 60 96000 57000 46000 33000 27000 11000 6700 80 58000 47000 31000 25000 16500 9300 4700

As can be seen in the above examples, by blowing ozone, not only shows a fast decomposition rate, but also it can be seen that the oligosaccharide is decomposed in a short time. The present invention can quickly produce low molecular weight polysaccharides and oligosaccharides efficiently, obtain pure low molecular weight polysaccharides, and are much more economical because the processing cost is low and the polysaccharides of desired molecular weight can be produced in a short time.

Claims (3)

A method for producing low molecular weight polysaccharides and oligosaccharides thereof comprising dissolving polysaccharides in a solvent and decomposing at a constant temperature while blowing a mixed gas of ozone / oxygen at a constant rate. The method for producing low molecular weight polysaccharide and oligosaccharide thereof according to claim 1, wherein the used ozone uses a mixture of ozone / oxygen and the amount of ozone is 0.01 to 10 times mole of the moles of glucoseamine units. The polysaccharide according to claim 1, wherein the polysaccharide includes pectin, chitin, chitosan, alginic acid, inulin, cellulose, starch, glycogen, heparin, hyaluronic acid, polygalactoseamine and derivatives thereof. Method of preparation.