KR19990000351A - Manufacturing method of pollen contents - Google Patents
Manufacturing method of pollen contents Download PDFInfo
- Publication number
- KR19990000351A KR19990000351A KR1019970023190A KR19970023190A KR19990000351A KR 19990000351 A KR19990000351 A KR 19990000351A KR 1019970023190 A KR1019970023190 A KR 1019970023190A KR 19970023190 A KR19970023190 A KR 19970023190A KR 19990000351 A KR19990000351 A KR 19990000351A
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- KR
- South Korea
- Prior art keywords
- pollen
- cell wall
- contents
- crushed
- water
- Prior art date
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- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 210000002421 cell wall Anatomy 0.000 claims abstract description 51
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000498 ball milling Methods 0.000 claims abstract description 11
- 238000010902 jet-milling Methods 0.000 claims abstract description 9
- 239000000284 extract Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 2
- 239000002245 particle Substances 0.000 abstract description 10
- 239000013566 allergen Substances 0.000 abstract description 9
- 206010020751 Hypersensitivity Diseases 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000007815 allergy Effects 0.000 abstract description 2
- 230000001079 digestive effect Effects 0.000 abstract description 2
- 208000026935 allergic disease Diseases 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 7
- 239000000843 powder Substances 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000257303 Hymenoptera Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 208000025966 Neurological disease Diseases 0.000 description 1
- 241000353135 Psenopsis anomala Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000002035 hexane extract Substances 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000007937 lozenge Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229940124595 oriental medicine Drugs 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229940109850 royal jelly Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
- A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/23—Removal of unwanted matter, e.g. deodorisation or detoxification by extraction with solvents
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Jellies, Jams, And Syrups (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
본 발명은 화분 내용물의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 화분의 세포벽을 볼 밀링이나 제트 밀링으로 파쇄시키고, 물 및/또는 헥산으로 화분 내용물을 추출한 다음, 알레르기 원(源)이 함유된 파쇄돤 화분 세포벽을 제거하여 화분 내용물을 제조하는 방법에 관한 것이다. 본 발명의 화분 내용물의 제조방법에 의하면, 화분 개개 입자의 단단한 세포벽을 파쇄하여 섭취시 소화 흡수율을 높일 뿐만 아니라, 알레르기원이 함유된 파쇄된 세포벽을 제거하므로 체내에서 알레르기를 유발하지 않는 화분 내용물만을 수집할 수 있다.The present invention relates to a method for producing pollen contents. More specifically, the present invention crushes the cell wall of the pollen by ball milling or jet milling, extracts the pollen contents with water and / or hexane, and then removes the crushed pollen cell wall containing allergens. It relates to a method of manufacturing. According to the production method of the pollen contents of the present invention, not only to increase the digestive absorption rate when ingested by breaking the hard cell wall of the individual pollen individual particles, but also to remove the crushed cell wall containing allergens, so that only the pollen contents that do not cause allergy in the body Can be collected.
Description
본 발명은 화분 내용물의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 화분의 세포벽을 볼 밀링이나 제트 밀링으로 파쇄시키고, 물 및/또는 헥산으로 화분 내용물을 추출한 다음, 알레르기 원(源)이 함유된 파쇄된 화분 세포벽을 제거하여 화분 내용물을 제조하는 방법에 관한 것이다.The present invention relates to a method for producing pollen contents. More specifically, the present invention crushes the cell wall of the pollen by ball milling or jet milling, extracts the pollen contents with water and / or hexane, and then removes the crushed pollen cell walls containing allergens. It relates to a method of manufacturing.
화분은 벌과 같은 곤충들에 의해 매개, 수집되는 충매화분과 바람에 의해 매개되는 풍매화분으로 나누어진다. 화분 영양 성분에 관한 일본 화분 영양 식품 협회나 스웨덴 AB세넬사 화분 연구소의 연구 보고들을 살펴보면, 화분의 종류에 따라 화분에는 수분 약 10∼15%, 조단백 약 20∼25%, 조지방 약 7∼15%, 조회분 약 2∼5%, 조섬유 약 3∼6%, 탄수화물 약 20∼40% 정도의 성분이 포함되어 있는 것으로 알려져 있다.Pollen is divided into hawk pollen, which is carried and collected by insects such as bees, and wind borne, which is collected by insects. According to research reports from the Japan Pollen Nutrition Food Association and Sweden AB Sennel Pollen Research Institute on pollen nutrition, pollen types contain about 10-15% moisture, 20-25% crude protein, and 7-15% crude fat. It is known to contain about 2 to 5% of crude ash, about 3 to 6% of crude fiber, and about 20 to 40% of carbohydrates.
또한, 화분에는 리신(Lysine)을 포함한 14종 이상의 많은 아미노산이 로얄제리의 10배 정도나 함유되어 있고, Mn, Si 등 미량 원소가 풍부하게 포함되어 있으며, 비타민 B그룹, 비타민C, 비타민D, 비타민E, 니코틴산, 판토텐산을 포함한 각종 비타민이 벌꿀이나 왕유보다 약 50∼400배 이상 함유되어 있고, 아밀레이즈(amylase)나 인버테이즈(invertase) 등의 효소가 다량 함유되어 있다. 동의보감 등의 한방과 임상 시험에서, 화분은 영양 효과, 정장 작용, 신경 장애, 동맥경화증, 노인병 등에 효과가 높아 그 영향학적 가치가 매우 높은 것으로 평가되고 있다.Also, pollen contains more than 14 kinds of amino acids including Lysine about 10 times of royal jelly, and contains abundant trace elements such as Mn, Si, vitamin B group, vitamin C, vitamin D, Various vitamins, including vitamin E, nicotinic acid, and pantothenic acid, contain about 50 to 400 times more than honey and royal oil, and contain large amounts of enzymes such as amylase and invertase. In oriental medicine and clinical trials such as Dongbobogam, pollen has a high effect on nutritional effects, formal function, neurological disorders, arteriosclerosis, geriatric disease, etc.
보통, 화분은 1개 입자의 크기가 10 내지 100 마이크로미터 정도로 매우 작아서 일반적인 물리적 방법으로는 화분을 파쇄시킬 수 없을 뿐만 아니라, 세포벽이 보통의 일반적인 식물 세포벽과는 달리, 펙토-셀룰로직(pecto-cellulogic) 성분으로 구성된 내피(intine)와 스포로폴레닌(sporopollenin, 산화 중합(oxidative polimerization)에 의해 형성된 카로티노이드 중합체)의 유기물로 구성된 외피(exine)로 이루어져 있기 때문에, 물리적·생물학적 분해작용에 대하여 매우 안정하며, 종종 화석으로 수천년 동안 세포벽이 전혀 손상받지 않은 상태로 발견되기도 한다.Usually, pollen is so small that the size of one particle is very small, such as 10 to 100 micrometers, so that the pollen cannot be crushed by normal physical methods, and the cell wall is different from the usual plant cell wall, pecto- Because it is composed of an intine composed of cellulogic components and an organic substance of sporopollenin (a carotenoid polymer formed by oxidative polimerization), it is very resistant to physical and biological degradation. It is stable and is often found in fossils without damage to the cell walls for thousands of years.
화분의 세포벽은 수박씨나 참외씨 같은 동물, 곤충, 꿀벌(성충)조차도 쉽게 분해하지 못하므로, 인체내에 섭취시 세포벽이 잘 분해되지 않고 체내 소화 흡수율이 매우 떨어져 화분 세포벽 내부의 우수한 영양성분들이 체내에 제대로 이용되지 못할 뿐 아니라, 세포벽에 알레르기 유발물질이 함유되어 있어서 일부 특정인들에게는 화분의 세포벽이 알레르기 원으로 작용한다.Pollen's cell walls are not easily decomposed by animals such as watermelon seeds or melon seeds, insects, and bees (adults). Not only is it poorly used, but the cell walls contain allergens, so in some people the pollen's cell wall acts as an allergen.
따라서, 화분을 안전하고 효율적인 식품이나 소재로 이용하기 위해서는 단단한 화분 세포벽을 파쇄시켜야 하며, 알레르기 원을 함유하고 있는 파쇄된 세포벽이 제거된 화분 내용물을 제조하는 방법이 당업계에서 절실히 요구되었다.Therefore, in order to use pollen as a safe and efficient food or material, it is necessary to crush the hard pollen cell wall, and there is an urgent need in the art for a method for producing pollen contents from which the crushed cell wall containing allergens is removed.
이에, 본 발명자들은 전술한 문제점을 해결하기 위하여 예의 연구노력한 결과, 볼 밀링 또는 제트 밀링의 물리적인 방법으로 화분의 세포벽을 파쇄하고, 파쇄 후 물과 헥산을 이용하여 화분 내용물을 추출한 다음, 인체 섭취시 특정인들에게 알레르기 원으로 작용하는 불용성의 세포벽을 내용물로부터 분리, 제거함으로써, 인체내 안정성이 확보된 화분 내용물이 제조됨을 확인하고, 본 발명을 완성하게 되었다.Therefore, the present inventors have made intensive studies to solve the above problems, and as a result of crushing the cell wall of the pollen by the physical method of ball milling or jet milling, extracting the contents of the pollen using water and hexane after the crushing, human body intake By separating and removing the insoluble cell wall, which acts as an allergen for the specific people, from the contents, it was confirmed that the potted contents of which the stability in the human body was secured were produced, and completed the present invention.
결국, 본 발명의 목적은 화분의 세포벽을 볼 밀링이나 제트 밀링으로 파쇄시키고, 물 및/또는 헥산으로 화분 내용물을 추출한 다음, 화분 세포벽을 제거하는 화분 내용물의 제조방법을 제공하는 것이다.After all, it is an object of the present invention to provide a method of producing pollen contents in which the cell walls of the pollen are crushed by ball milling or jet milling, the pollen contents are extracted with water and / or hexane, and then the pollen cell walls are removed.
제1도는 화분 내용물의 제조방법을 공정별로 나타낸 개략도이다.1 is a schematic diagram showing a method of manufacturing the potted contents.
제2도는 세포벽이 파쇄된 화분으로부터 내용물을 추출하고 세포벽을 제거하는 공정을 나타내는 개략도이다.2 is a schematic diagram showing the process of extracting the contents from the pollen where the cell wall is broken and removing the cell wall.
이하, 본 발명의 화분 세포벽 파쇄 및 그의 내용물 제조방법을 공정에 따라 상세히 설명하기로 한다.Hereinafter, the method for producing pollen cell wall crushing and its contents will be described in detail according to the process.
제1공정: 화분의 조 분쇄 공정1st step: crude grinding process of pot
덩어리 모양의 화분하(荷)로 수집되는 잡화분, 도토리화분 등의 충매화분은 미리 분말 상태로 조 분쇄하여 덩어리가 개개의 화분 입자로 되게 하고, 분말상태로 수집되는 풍매화분에는 조 분쇄 공정을 적용하지 않는다. 상기에서, 총매화분은 컷팅 밀이나 로울러 밀에 의해 조 분쇄되어 60메쉬(mesh) 체를 통과하도록 분말화되는 것이 바람직하다.Lozenges such as sundries and acorns, which are collected under a lump-like pollen, are coarsely pulverized in powder form in advance, so that the lumps become individual pollen particles. Does not apply In the above, the total plum is coarsely pulverized by a cutting mill or roller mill, and powdered to pass through a 60 mesh sieve.
제2공정: 세포벽 파쇄 공정Second Process: Cell Wall Crushing Process
분말상의 풍매화분이나 제1공정에서 조 분쇄된 충매화분의 세포벽을 볼 밀링이나 제트 밀링으로 파쇄시키고, 현미경이나 입도분석기를 사용하여 파쇄정도를 측정한다.The cell walls of the powdered aerated powder or the crudely pulverized powdered powder in the first step are crushed by ball milling or jet milling, and the degree of crushing is measured using a microscope or a particle size analyzer.
제3공정: 내용물 추출 및 세포벽 제거 공정3rd step: content extraction and cell wall removal
제2공정에서 세포벽이 파쇄된 화분에 헥산 및 물 용매를 독립적으로 또는 연속적으로 첨가하여 지용성 및/또는 수용성 성분을 추출한 다음, 이 추출액에 혼합되어 있는 불용성의 파쇄 세포벽을 원심분리에 의해 침전물로 제거시켜 화분 내용물을 제조한다.In the second step, hexane and water solvent are added to the potted cell wall independently or continuously to extract fat-soluble and / or water-soluble components, and then the insoluble broken cell wall mixed in the extract is removed as a precipitate by centrifugation. To make potted contents.
상기에서, 헥산 및 물에 의한 연속 추출은 세포벽이 파쇄된 화분에 헥산을 가하여, 가장 바람직하게는 파쇄된 화분에 대하여 5배 중량비로 헥산을 가하여 지용성 성분을 추출한 다음, 원심분리하여 얻은 침전물에 물을 첨가하여, 가장 바람직하게는 침전물에 대하여 5배 중량비로 물을 가하여 수용성 성분을 추출하는 일련의 연속과정에 의해 진행된다.In the above, continuous extraction by hexane and water is added to the hexane to the cell wall crushed potted plant, most preferably by adding hexane to the weight ratio 5 times with respect to the crushed potted plant to extract the fat-soluble components, and then centrifuged to precipitate the water It is most preferably carried out by a series of continuous processes in which water is added to the precipitate in an amount of 5 times the weight ratio to extract the water-soluble components.
이상과 같은 화분의 세포벽 파쇄 및 화분 내용물의 제조공정은 제1도에 개략적으로 나타내었다.The cell wall crushing of the pollen and the manufacturing process of the pollen contents as shown above are schematically shown in FIG.
이하, 본 발명을 실시예에 의하여 더욱 구체적으로 설명하고자 한다. 이들 실시예는 오로지 본 발명을 구체적으로 설명하는 것으로, 이들 실시예에 의해 본 발명의 범위가 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자들에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only to specifically describe the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples.
[실시예 1] 화분의 조 분쇄Example 1 Crude grinding of pots
분말 상태로 수집되는 풍매화분인 송화분은 조 분쇄 공정이 필요 없지만, 화분하 형태로 수집되는 충매화분에 속하는 잡화분 및 도토리화분은 컷팅 밀이나 로울러 밀을 사용하여 60메쉬(mesh) 체를 통과하도록 조 분쇄하여, 덩어리가 개개의 화분 입자들로 분리되도록 하였다.The pine pollen, which is collected in the form of powder, does not require a coarse grinding process, but the miscellaneous and acorn pollen belonging to the monofilament collected in the form of pollen are passed through a 60 mesh sieve using a cutting mill or roller mill. Crude crushing allowed the mass to separate into individual pollen particles.
[실시예 2] 볼 밀링 또는 제트 밀링에 의한 세포벽 파쇄Example 2 Cell Wall Fracture by Ball Milling or Jet Milling
분말상의 송화분이나, 조 분쇄된 잡화분 및 도토리화분의 세포벽을 볼 밀링으로 파쇄하고, 파쇄정도를 현미경으로 관찰한 결과를 하기 표 1에 나타내었다. 파쇄되기전의 화분은 둥근 원형이나, 세포벽 파쇄가 진행될수록 부정형으로 작게 잘라짐을 현미경으로 관찰할 수 있었다.Table 1 shows the results of observing the cell walls of the powdered pine ash, crude crushed miscellaneous powder and acorn powder by ball milling and observing the degree of crushing under a microscope. The pollen before crushing was round and circular, but as cell wall rupture proceeded, it could be observed under a microscope to be cut into small irregular shapes.
[표 1] 볼 밀링에 의한 화분의 세포벽 파쇄정도(*)[Table 1] Cell wall crushing degree of pollen by ball milling (*)
*:-는 전혀 파쇄가 안되었음, +는 매우 약간 파쇄되었음, ++는 약간 파쇄되었음, +++는 반정도 파쇄되었음, ++++는 거의 파쇄되었음, +++++는 완전히 파쇄되었음을 각각 나타낸다.*:-Was not broken at all, + was very slightly broken, ++ was slightly broken, +++ was half broken, ++++ was almost broken, +++++ was completely broken Respectively.
상기 표 1에서 보듯이, 송화분은 약 40시간, 잡화분과 도토리화분은 약 50시간만에 완전히 세포벽이 파쇄됨을 알 수 있었다.As shown in Table 1, it can be seen that the cell wall is completely disrupted in about 40 hours, miscellaneous and acorn flower in about 50 hours.
아울러, 공기압력 3 내지 10Kg/㎠G, 투입속도 1 내지 25Kg/hr의 운전 조건에서 제트 밀링 방식으로 상술한 화분의 세포벽을 파쇄한 결과, 화분의 세포벽이 완전히 파쇄됨을 현미경 관찰로 확인하였다.In addition, as a result of crushing the cell wall of the potted plant described above by jet milling under the operating conditions of air pressure 3 to 10Kg / ㎠G, feed rate 1 to 25Kg / hr, it was confirmed by microscopic observation that the cell wall of the potted plant completely broken.
한편, 약 40시간 동안 볼 밀링한 송화분, 약 50시간 동안 볼 밀링한 잡화분과 도토리화분의 세포벽 파쇄를 입도분석기를 사용한 입자의 크기로서 조사한 결과(참조: 표 2), 파쇄 전보다 파쇄 후의 입자가 매우 작게 나타나 볼 밀링에 의해서 화분의 세포벽이 완전히 파쇄되었음을 알 수 있었다.On the other hand, the cell wall crushing of the ball milled ball mill for about 40 hours, the miscellaneous ball mill and the acorn plant milled for about 50 hours was examined as the particle size using the particle size analyzer (see Table 2). It appeared small that the cell wall of the pollen was completely crushed by ball milling.
[표 2] 파쇄 전 화분과 볼 밀링에 의해 파쇄된 화분의 평균 입자 크기Table 2 Average particle size of pots crushed by pot milling and ball milling before crushing
아울러, 전술한 운전 조건하에서 제트 밀링 방식으로 화분의 세포벽을 파쇄한 결과, 파쇄 전보다 파쇄 후의 입자가 더 작게 나타나 제트 밀링에 의해서 화분의 세포벽이 완전히 파쇄되었음을 확인하였다.In addition, as a result of crushing the cell wall of the pollen by the jet milling method under the above operating conditions, it was confirmed that the particles after crushing are smaller than before crushing, so that the cell wall of the pollen was completely crushed by jet milling.
[실시예 3] 내용물 추출 및 세포벽 제거Example 3 Content Extraction and Cell Wall Removal
세포벽이 완전히 파쇄된 화분으로부터 알레르기 원이 함유된 세포벽을 제거하고 유용한 내용물만을 추출하기 위해 본 실시예에서 확립된 공정은 제2도에 나타내었다. 제2도에 개시된 바와 같이, 세포벽이 파쇄된 화분의 내용물을 물 추출물과 헥산 추출물 형태로 제조한 결과, 총 추출 수율(물 가용성 고형분양 + 헥산 가용성 고형분양)이 잡화분 80.6%(w/w), 도토리화분 92.6%(w/w), 송화분 62.5%(w/w)로 매우 높게 나타났으며, 추출물 총량중 물 추출물에만 약 90%(w/w) 이상이 회수되었다. 파쇄된 세포벽은 불용성으로서 추출후 원심분리에 의해서 침전물 형태로 제거시켰다.The process established in this example is shown in FIG. 2 to remove the allergen containing cell wall and extract only the useful contents from the pollen where the cell wall is completely broken. As disclosed in FIG. 2, the contents of the potted plants whose cell walls were crushed were prepared in the form of water extract and hexane extract. As a result, the total extraction yield (water soluble solid content + hexane soluble solid content) was 80.6% (w / w). ), Acorns 92.6% (w / w), pine pollen 62.5% (w / w) was very high, and more than about 90% (w / w) was recovered only in the water extract of the total extract. The crushed cell wall was insoluble and removed in precipitate form by centrifugation after extraction.
이상에서 상세히 설명하고 입증하였듯이, 본 발명의 화분 내용물의 제조방법에 의하면, 화분 개개 입자의 단단한 세포벽을 파쇄하여 섭취시 소화 흡수율을 높일 뿐만 아니라, 알레르기원이 함유된 파쇄된 세포벽을 제거하므로 체내에서 알레르기를 유발하지 않는 화분 내용물만을 수집할 수 있다.As described and demonstrated in detail above, according to the method for producing the pollen contents of the present invention, by breaking the solid cell wall of the individual pollen particles to increase digestive absorption rate when ingested, as well as remove the broken cell wall containing allergens in the body You can only collect pollen contents that do not cause allergies.
Claims (4)
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| KR1019970023190A KR100211287B1 (en) | 1997-06-04 | 1997-06-04 | Processing method of pollen |
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| KR1019970023190A KR100211287B1 (en) | 1997-06-04 | 1997-06-04 | Processing method of pollen |
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| DE102012200836B3 (en) * | 2012-01-20 | 2013-08-01 | Apotheke am Prediger Monika Biechele e. Kfr. | Digesting flower pollen useful for treating allergic symptoms, e.g. sneezing and watery eyes, comprises providing pollen to be broken down in mixture with lactose and circulating such that lactose particles are able to cut open pollen |
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