KR102921926B1 - 중합효소, 조성물, 및 사용 방법 - Google Patents
중합효소, 조성물, 및 사용 방법Info
- Publication number
- KR102921926B1 KR102921926B1 KR1020207037274A KR20207037274A KR102921926B1 KR 102921926 B1 KR102921926 B1 KR 102921926B1 KR 1020207037274 A KR1020207037274 A KR 1020207037274A KR 20207037274 A KR20207037274 A KR 20207037274A KR 102921926 B1 KR102921926 B1 KR 102921926B1
- Authority
- KR
- South Korea
- Prior art keywords
- glu
- lys
- leu
- val
- arg
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862753558P | 2018-10-31 | 2018-10-31 | |
| US62/753,558 | 2018-10-31 | ||
| PCT/US2019/059246 WO2020092830A1 (en) | 2018-10-31 | 2019-10-31 | Polymerases, compositions, and methods of use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20210084349A KR20210084349A (ko) | 2021-07-07 |
| KR102921926B1 true KR102921926B1 (ko) | 2026-02-04 |
Family
ID=68621372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020207037274A Active KR102921926B1 (ko) | 2018-10-31 | 2019-10-31 | 중합효소, 조성물, 및 사용 방법 |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US11104888B2 (https=) |
| EP (1) | EP3874036A1 (https=) |
| JP (1) | JP7515413B2 (https=) |
| KR (1) | KR102921926B1 (https=) |
| CN (1) | CN112673098B (https=) |
| AU (1) | AU2019374098B2 (https=) |
| BR (1) | BR112020026658A2 (https=) |
| CA (1) | CA3103719A1 (https=) |
| IL (2) | IL299237B2 (https=) |
| MX (1) | MX2020013347A (https=) |
| MY (1) | MY203805A (https=) |
| SG (1) | SG11202012493WA (https=) |
| WO (1) | WO2020092830A1 (https=) |
| ZA (1) | ZA202007838B (https=) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3874036A1 (en) * | 2018-10-31 | 2021-09-08 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| WO2020117968A2 (en) | 2018-12-05 | 2020-06-11 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| JP2023510454A (ja) | 2019-11-27 | 2023-03-14 | イルミナ ケンブリッジ リミテッド | シクロオクタテトラエンを含有する色素及び組成物 |
| EP4081653B1 (en) | 2019-12-23 | 2024-07-03 | Singular Genomics Systems, Inc. | Methods for long read sequencing |
| AU2020416718A1 (en) | 2019-12-31 | 2022-07-07 | Singular Genomics Systems, Inc. | Polynucleotide barcodes for long read sequencing |
| US11174281B1 (en) | 2020-04-24 | 2021-11-16 | Singular Genomics Systems, Inc. | Modified nucleotides and uses thereof |
| US11787831B2 (en) | 2020-06-22 | 2023-10-17 | Illumina Cambridge Limited | Nucleosides and nucleotides with 3′ acetal blocking group |
| US20220195516A1 (en) | 2020-12-17 | 2022-06-23 | Illumina Cambridge Limited | Methods, systems and compositions for nucleic acid sequencing |
| US20220195518A1 (en) | 2020-12-22 | 2022-06-23 | Illumina Cambridge Limited | Methods and compositions for nucleic acid sequencing |
| US12054506B2 (en) | 2021-03-19 | 2024-08-06 | Singular Genomics Systems, Inc. | Reducing agents and uses thereof |
| MX2023011660A (es) * | 2021-03-31 | 2023-12-11 | Illumina Cambridge Ltd | Tecnicas de secuenciacion de genoteca de acido nucleico con deteccion de dimero adaptador. |
| JP2024516191A (ja) | 2021-05-05 | 2024-04-12 | イルミナ ケンブリッジ リミテッド | ビス－ホウ素縮合複素環を含有する蛍光色素及び配列決定における使用 |
| KR20240009435A (ko) | 2021-05-20 | 2024-01-22 | 일루미나, 인코포레이티드 | 합성에 의한 시퀀싱을 위한 조성물 및 방법 |
| US20220403351A1 (en) | 2021-06-18 | 2022-12-22 | Element Biosciences, Inc. | Engineered polymerases |
| US12077789B2 (en) | 2021-08-14 | 2024-09-03 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11788075B2 (en) | 2022-02-18 | 2023-10-17 | Element Biosciences, Inc. | Engineered polymerases with reduced sequence-specific errors |
| US20230304086A1 (en) | 2022-03-28 | 2023-09-28 | Illumina Cambridge Limited | Labeled avidin and methods for sequencing |
| AU2023246772A1 (en) | 2022-03-29 | 2024-01-18 | Illumina Inc. | Chromenoquinoline dyes and uses in sequencing |
| CA3223037A1 (en) | 2022-03-30 | 2023-10-05 | Oliver MILLER | Methods for chemical cleavage of surface-bound polynucleotides |
| US20230357845A1 (en) | 2022-03-31 | 2023-11-09 | Illumina, Inc. | Compositions and methods for improving sequencing signals |
| WO2023192900A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Singapore Pte. Ltd. | Nucleosides and nucleotides with 3' vinyl blocking group useful in sequencing by synthesis |
| CN119301275A (zh) | 2022-05-31 | 2025-01-10 | 伊路米纳有限公司 | 用于核酸测序的组合物和方法 |
| CN119604513A (zh) | 2022-06-28 | 2025-03-11 | 因美纳公司 | 含有稠合四环双硼杂环的荧光染料及其在测序中的用途 |
| US20240140939A1 (en) | 2022-09-30 | 2024-05-02 | Illumina Cambridge Limited | Compositions and methods for reducing photo damage during sequencing |
| US20240141427A1 (en) | 2022-09-30 | 2024-05-02 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| WO2024123866A1 (en) | 2022-12-09 | 2024-06-13 | Illumina, Inc. | Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers |
| CN119095982A (zh) | 2022-12-22 | 2024-12-06 | 伊路米纳有限公司 | 用于边合成边测序的钯催化剂组合物和方法 |
| WO2024137765A1 (en) | 2022-12-22 | 2024-06-27 | Illumina, Inc. | Transition-metal catalyst compositions and methods for sequencing by synthesis |
| CN119095984A (zh) | 2022-12-27 | 2024-12-06 | 伊路米纳有限公司 | 使用3'烯丙基封端的核苷酸的测序方法 |
| CN118556118A (zh) * | 2023-01-04 | 2024-08-27 | 中元汇吉生物技术股份有限公司 | 一种dna聚合酶突变体、制备及其应用 |
| WO2024206407A2 (en) | 2023-03-29 | 2024-10-03 | Illumina, Inc. | Naphthalimide dyes and uses in nucleic acid sequencing |
| EP4689181A1 (en) | 2023-03-30 | 2026-02-11 | Illumina, Inc. | Compositions and methods for nucleic acid sequencing |
| EP4720334A1 (en) | 2023-05-26 | 2026-04-08 | Illumina, Inc. | Methods for preserving methylation status during clustering |
| WO2024249061A1 (en) | 2023-05-31 | 2024-12-05 | Illumina, Inc. | Detecting a methylated nucleotide using a quencher |
| CN121241132A (zh) * | 2023-06-02 | 2025-12-30 | 武汉华大智造科技有限公司 | 用于测序的dna聚合酶突变体 |
| WO2025049700A1 (en) | 2023-08-31 | 2025-03-06 | Illumina, Inc. | Compositions and methods for nucleic acid sequencing |
| WO2025090596A1 (en) | 2023-10-26 | 2025-05-01 | Illumina, Inc. | 4,5-substituted naphthalimide dyes and uses in nucleic acid sequencing |
| US20250163505A1 (en) | 2023-11-17 | 2025-05-22 | Illumina, Inc. | Conjugated polymers or polymer dots as fluorescent labels |
| US20250197933A1 (en) | 2023-12-18 | 2025-06-19 | Illumina, Inc. | Hydrogel nanoparticles as labeling scaffold in sequencing |
| WO2025144716A1 (en) | 2023-12-28 | 2025-07-03 | Illumina, Inc. | Nucleotides with enzymatically cleavable 3'-o-glycoside blocking groups for sequencing |
| US20250215494A1 (en) | 2023-12-29 | 2025-07-03 | Illumina, Inc. | Tricyclic polymethine dyes for nucleic acid sequencing |
| WO2025184226A1 (en) | 2024-02-28 | 2025-09-04 | Illumina, Inc. | Nucleotides with terminal phosphate capping |
| WO2025188906A1 (en) | 2024-03-08 | 2025-09-12 | Illumina, Inc. | Modified adenosine nucleotides |
| WO2025230914A1 (en) | 2024-04-29 | 2025-11-06 | Illumina, Inc. | Nucleotides with enzyme-triggered self-immolative linkers for sequencing by synthesis |
| WO2025264831A1 (en) | 2024-06-18 | 2025-12-26 | Illumina, Inc. | Methods for increasing sequencing quality of gc-rich regions |
| WO2025264836A1 (en) | 2024-06-18 | 2025-12-26 | Illumina, Inc. | Methods for increasing sequencing quality of gc-rich regions |
| WO2026006314A1 (en) | 2024-06-28 | 2026-01-02 | Illumina, Inc. | Tagging target regions prior to nucleotide sequencing |
| WO2026035914A1 (en) | 2024-08-09 | 2026-02-12 | Illumina, Inc. | Nucleotides having 3'-hydroxy blocking groups |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006037064A2 (en) | 2004-09-24 | 2006-04-06 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
Family Cites Families (74)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| US5756334A (en) | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
| WO1997039150A1 (en) | 1996-04-15 | 1997-10-23 | University Of Southern California | Synthesis of fluorophore-labeled dna |
| EP0822256B1 (en) | 1996-07-29 | 2003-09-24 | Toyo Boseki Kabushiki Kaisha | Modified thermostable DNA polymerase, and DNA polymerase composition for nucleic acid amplification |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| US6228628B1 (en) | 1997-07-09 | 2001-05-08 | Roche Molecular Systems | Mutant chimeric DNA polymerase |
| US5882904A (en) | 1997-08-04 | 1999-03-16 | Amersham Pharmacia Biotech Inc. | Thermococcus barossii DNA polymerase mutants |
| US5948666A (en) | 1997-08-06 | 1999-09-07 | Diversa Corporation | Isolation and identification of polymerases |
| US20050244870A1 (en) | 1999-04-20 | 2005-11-03 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
| JP4150166B2 (ja) | 1999-05-07 | 2008-09-17 | ロシュ ダイアグノスティックス ゲーエムベーハー | 修飾ヌクレオチドまたは発色団を有するヌクレオチドとｄｎａポリメラーゼを使用するｄｎａの高密度標識 |
| US6492161B1 (en) | 1999-06-02 | 2002-12-10 | Prokaria Ltd. | Bacteriophage RM 378 of a thermophilic host organism |
| US7244559B2 (en) | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| JP2003510063A (ja) | 1999-09-30 | 2003-03-18 | ニュー・イングランド・バイオラブズ・インコーポレイティッド | 古細菌のｄｎａポリメラーゼによる修飾ヌクレオチドの取り込み及びそれに関する方法 |
| US20030228616A1 (en) | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| US8268605B2 (en) * | 1999-10-29 | 2012-09-18 | Agilent Technologies, Inc. | Compositions and methods utilizing DNA polymerases |
| DE60027040T2 (de) | 1999-10-29 | 2006-11-23 | Stratagene California, La Jolla | Zusammensetzungen und methoden zur verwendung von dna polymerasen |
| WO2001038546A1 (en) | 1999-11-23 | 2001-05-31 | Amersham Biosciences Corp | Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| AU2007201277B2 (en) | 2000-05-24 | 2011-10-13 | Third Wave Technologies, Inc. | Detection of RNA |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| AU2001279134A1 (en) | 2000-07-31 | 2002-02-13 | Maxygen, Inc. | Nucleotide incorporating enzymes |
| AU2002227156A1 (en) | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| EP1395805A4 (en) | 2001-06-11 | 2005-03-09 | Illumina Inc | MULTIPLEXED DETECTION TECHNIQUES |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| US8283148B2 (en) | 2002-10-25 | 2012-10-09 | Agilent Technologies, Inc. | DNA polymerase compositions for quantitative PCR and methods thereof |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| CA2579150C (en) | 2004-09-17 | 2014-11-25 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| EP1888743B1 (en) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Improved polymerases |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| CA2633524A1 (en) | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
| CA2648149A1 (en) | 2006-03-31 | 2007-11-01 | Solexa, Inc. | Systems and devices for sequence by synthesis analysis |
| EP2044188B1 (en) | 2006-07-14 | 2015-09-09 | Novozymes A/S | Polypeptides having lipase activity and polynucleotides encoding same |
| ES2404665T3 (es) | 2006-09-06 | 2013-05-28 | Medical Research Council | Polimerasas de ADN que incorporan análogos de nucleótidos marcados con colorante |
| GB0617575D0 (en) | 2006-09-06 | 2006-10-18 | Syngenta Ltd | Herbicidal compounds and compositions |
| US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
| US9085762B2 (en) | 2006-12-29 | 2015-07-21 | Agilent Technologies, Inc. | Compositions and methods using split polymerases |
| EP2231878B1 (en) | 2007-12-27 | 2012-12-26 | GE Healthcare Bio-Sciences Corp. | A single enzyme system for fast, ultra long pcr |
| US20110045489A1 (en) | 2008-04-22 | 2011-02-24 | New England Biolabs, Inc. | Polymerases for Incorporating Modified Nucleotides |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| CA2742593C (en) * | 2008-11-03 | 2020-05-05 | Kapa Biosystems, Inc. | Chimeric dna polymerases |
| US8481685B2 (en) | 2008-11-03 | 2013-07-09 | Kapa Biosystems | Modified DNA polymerases |
| EP2388322B1 (en) | 2009-01-15 | 2017-10-25 | Hokkaido Mitsui Chemicals, Inc. | Enzymatic preparation containing thermostable dna polymerase, process for producing same, and method for detecting analyte organism |
| WO2011026194A1 (en) | 2009-09-07 | 2011-03-10 | Reproductive Health Science Pty Ltd | Nucleic acid extraction |
| US8965076B2 (en) | 2010-01-13 | 2015-02-24 | Illumina, Inc. | Data processing system and methods |
| US20110301041A1 (en) * | 2010-02-26 | 2011-12-08 | Life Technologies Corporation | Modified Proteins and Methods of Making and Using Same |
| GB201007384D0 (en) | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
| EP2632593B1 (en) | 2010-10-27 | 2021-09-29 | Illumina, Inc. | Flow cells for biological or chemical analysis |
| US8921044B2 (en) | 2011-05-11 | 2014-12-30 | New England Biolabs, Inc. | DNA polymerase variants with reduced exonuclease activity and uses thereof |
| US8921043B2 (en) | 2011-05-11 | 2014-12-30 | New England Biolabs, Inc. | DNA polymerase variants with reduced exonuclease activity and uses thereof |
| EP2971070B2 (en) | 2013-03-14 | 2021-03-03 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| GB2526261B (en) | 2014-04-28 | 2017-08-02 | Gelliner Ltd | Encoded cells and cell arrays |
| EP3702471B1 (en) | 2014-06-27 | 2025-12-24 | Illumina, Inc. | Modified polymerases for improved incorporation of nucleotide analogues |
| EP3778891A1 (en) | 2014-08-27 | 2021-02-17 | New England Biolabs, Inc. | Synthon formation |
| CN107002051A (zh) | 2014-09-30 | 2017-08-01 | 亿明达股份有限公司 | 用于核苷酸类似物的改善的掺入的经修饰的聚合酶 |
| US20200231947A1 (en) | 2015-09-09 | 2020-07-23 | Qiagen Gmbh | Polymerase enzyme |
| US10662413B2 (en) * | 2016-11-01 | 2020-05-26 | Personal Genomics, Inc. | Recombinant DNA polymerase for improved incorporation of nucleotide analogues |
| EP3613851A4 (en) | 2017-01-09 | 2020-07-01 | MGI Tech Co., Ltd. | RECOMBINANT DNA POLYMERASE |
| WO2018148727A1 (en) | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from 9°n |
| WO2018148723A1 (en) | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from pyrococcus abyssi |
| WO2018148726A1 (en) | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from phage t4 |
| EP3580351A1 (en) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymerase enzyme from 9°n |
| WO2018148724A1 (en) | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from pyrococcus furiosus |
| CN112639089B (zh) | 2018-09-03 | 2024-02-09 | 深圳华大生命科学研究院 | 重组型kod聚合酶 |
| US20230011240A1 (en) | 2018-09-11 | 2023-01-12 | Singular Genomics Systems, Inc. | Modified archaeal family b polymerases |
| EP3853358A1 (en) | 2018-09-17 | 2021-07-28 | Omniome, Inc. | Engineered polymerases for improved sequencing |
| EP3874036A1 (en) | 2018-10-31 | 2021-09-08 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| WO2020117968A2 (en) | 2018-12-05 | 2020-06-11 | Illumina, Inc. | Polymerases, compositions, and methods of use |
-
2019
- 2019-10-31 EP EP19806368.7A patent/EP3874036A1/en active Pending
- 2019-10-31 SG SG11202012493WA patent/SG11202012493WA/en unknown
- 2019-10-31 MX MX2020013347A patent/MX2020013347A/es unknown
- 2019-10-31 US US16/670,876 patent/US11104888B2/en active Active
- 2019-10-31 KR KR1020207037274A patent/KR102921926B1/ko active Active
- 2019-10-31 AU AU2019374098A patent/AU2019374098B2/en active Active
- 2019-10-31 BR BR112020026658-2A patent/BR112020026658A2/pt not_active IP Right Cessation
- 2019-10-31 CN CN201980043453.3A patent/CN112673098B/zh active Active
- 2019-10-31 WO PCT/US2019/059246 patent/WO2020092830A1/en not_active Ceased
- 2019-10-31 JP JP2020572427A patent/JP7515413B2/ja active Active
- 2019-10-31 IL IL299237A patent/IL299237B2/en unknown
- 2019-10-31 MY MYPI2020006917A patent/MY203805A/en unknown
- 2019-10-31 CA CA3103719A patent/CA3103719A1/en active Pending
-
2020
- 2020-12-13 IL IL279422A patent/IL279422B2/en unknown
- 2020-12-15 ZA ZA2020/07838A patent/ZA202007838B/en unknown
-
2021
- 2021-08-09 US US17/397,003 patent/US11560552B2/en active Active
-
2022
- 2022-12-21 US US18/085,674 patent/US20230212534A1/en not_active Abandoned
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006037064A2 (en) | 2004-09-24 | 2006-04-06 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
| US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
Also Published As
| Publication number | Publication date |
|---|---|
| ZA202007838B (en) | 2023-04-26 |
| BR112020026658A2 (pt) | 2021-08-10 |
| AU2019374098B2 (en) | 2025-10-16 |
| KR20210084349A (ko) | 2021-07-07 |
| US11560552B2 (en) | 2023-01-24 |
| US11104888B2 (en) | 2021-08-31 |
| IL299237A (en) | 2023-02-01 |
| JP7515413B2 (ja) | 2024-07-12 |
| JP2022503427A (ja) | 2022-01-12 |
| WO2020092830A1 (en) | 2020-05-07 |
| SG11202012493WA (en) | 2021-01-28 |
| IL279422B2 (en) | 2023-05-01 |
| IL299237B2 (en) | 2024-12-01 |
| CN112673098A (zh) | 2021-04-16 |
| MY203805A (en) | 2024-07-19 |
| US20220056424A1 (en) | 2022-02-24 |
| MX2020013347A (es) | 2021-04-28 |
| IL299237B1 (en) | 2024-08-01 |
| IL279422A (en) | 2021-01-31 |
| EP3874036A1 (en) | 2021-09-08 |
| AU2019374098A1 (en) | 2021-01-07 |
| IL279422B1 (en) | 2023-01-01 |
| US20230212534A1 (en) | 2023-07-06 |
| CA3103719A1 (en) | 2020-05-07 |
| CN112673098B (zh) | 2023-01-06 |
| US20200131484A1 (en) | 2020-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102921926B1 (ko) | 중합효소, 조성물, 및 사용 방법 | |
| US11634697B2 (en) | Polymerases, compositions, and methods of use | |
| AU2021290289B2 (en) | Modified Polymerases For Improved Incorporation Of Nucleotide Analogues | |
| US10696955B2 (en) | Modified polymerases for improved incorporation of nucleotide analogues | |
| US12077789B2 (en) | Polymerases, compositions, and methods of use | |
| RU2779599C1 (ru) | Полимеразы, композиции и способы их применения | |
| HK1235089A1 (en) | Modified polymerases for improved incorporation of nucleotide analogues | |
| HK1235089B (en) | Modified polymerases for improved incorporation of nucleotide analogues |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
|
| A201 | Request for examination | ||
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |
|
| PN2301 | Change of applicant |
St.27 status event code: A-3-3-R10-R13-asn-PN2301 St.27 status event code: A-3-3-R10-R11-asn-PN2301 |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |
|
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| D22 | Grant of ip right intended |
Free format text: ST27 STATUS EVENT CODE: A-1-2-D10-D22-EXM-PE0701 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| PE0701 | Decision of registration |
St.27 status event code: A-1-2-D10-D22-exm-PE0701 |
|
| F11 | Ip right granted following substantive examination |
Free format text: ST27 STATUS EVENT CODE: A-2-4-F10-F11-EXM-PR0701 (AS PROVIDED BY THE NATIONAL OFFICE) |
|
| PR0701 | Registration of establishment |
St.27 status event code: A-2-4-F10-F11-exm-PR0701 |
|
| PR1002 | Payment of registration fee |
St.27 status event code: A-2-2-U10-U12-oth-PR1002 Fee payment year number: 1 |
|
| U12 | Designation fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U12-OTH-PR1002 (AS PROVIDED BY THE NATIONAL OFFICE) Year of fee payment: 1 |
|
| PG1601 | Publication of registration |
St.27 status event code: A-4-4-Q10-Q13-nap-PG1601 |
|
| Q13 | Ip right document published |
Free format text: ST27 STATUS EVENT CODE: A-4-4-Q10-Q13-NAP-PG1601 (AS PROVIDED BY THE NATIONAL OFFICE) |