KR102700433B1 - Aav의 제조 방법 - Google Patents

Aav의 제조 방법 Download PDF

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Publication number
KR102700433B1
KR102700433B1 KR1020227040149A KR20227040149A KR102700433B1 KR 102700433 B1 KR102700433 B1 KR 102700433B1 KR 1020227040149 A KR1020227040149 A KR 1020227040149A KR 20227040149 A KR20227040149 A KR 20227040149A KR 102700433 B1 KR102700433 B1 KR 102700433B1
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aav
perfusion
production
cell
cells
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Korean (ko)
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KR20220164801A (ko
Inventor
율리아나 코로넬
실케 비씽
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세벡 파마세우티컬스 게엠베하
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020227040149A 2020-11-25 2021-11-23 Aav의 제조 방법 Active KR102700433B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20209811.7A EP4006141A1 (en) 2020-11-25 2020-11-25 Method for the production of aav
EP20209811.7 2020-11-25
PCT/EP2021/082615 WO2022112218A1 (en) 2020-11-25 2021-11-23 Method for the production of aav

Publications (2)

Publication Number Publication Date
KR20220164801A KR20220164801A (ko) 2022-12-13
KR102700433B1 true KR102700433B1 (ko) 2024-08-30

Family

ID=73597941

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020227040149A Active KR102700433B1 (ko) 2020-11-25 2021-11-23 Aav의 제조 방법

Country Status (15)

Country Link
US (1) US20240263148A1 (https=)
EP (3) EP4006141A1 (https=)
JP (2) JP7601339B2 (https=)
KR (1) KR102700433B1 (https=)
CN (1) CN116209748A (https=)
AU (1) AU2021388811B2 (https=)
CA (1) CA3174017C (https=)
DK (1) DK4107252T3 (https=)
ES (1) ES3017247T3 (https=)
FI (1) FI4107252T3 (https=)
IL (1) IL296770B2 (https=)
MX (1) MX2022013385A (https=)
PT (1) PT4107252T (https=)
WO (1) WO2022112218A1 (https=)
ZA (1) ZA202210745B (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2022373653A1 (en) * 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
CN119137263A (zh) * 2022-03-07 2024-12-13 奥特吉尼克斯制药公司 改进的分批aav产生系统和方法
EP4532737A1 (en) * 2022-06-03 2025-04-09 F. Hoffmann-La Roche AG Method for producing recombinant aav particles
KR20260048589A (ko) 2023-08-29 2026-04-10 후지필름 가부시키가이샤 바이러스의 제조 방법 및 세포로의 핵산 도입 방법
WO2025171001A1 (en) 2024-02-06 2025-08-14 Regeneron Pharmaceuticals, Inc. Compositions and methods for improved production of aav viral vectors
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
EP4606816A1 (en) 2024-02-26 2025-08-27 Universitat Autónoma De Barcelona (UAB) Method for production of gene therapy vehicles in mammalian cells
WO2025226558A1 (en) 2024-04-22 2025-10-30 Ultragenyx Pharmaceutical Inc. Multi-step filtration and clarification methods for recombinant adeno-associated virus purification
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna
WO2026010979A2 (en) * 2024-07-01 2026-01-08 Genzyme Corporation Perfusion in production bioreactors
US20260022401A1 (en) 2024-07-22 2026-01-22 Hoffmann-La Roche Inc. Novel AAV rep ORFs and Rep polypeptides
WO2026037757A1 (en) 2024-08-13 2026-02-19 F. Hoffmann-La Roche Ag Modified aav particles

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127582A1 (en) * 1997-09-05 2002-09-12 Atkinson Edward M. Methods for generating high titer helper-free preparations of released recombinant AAV vectors
WO2020154607A1 (en) * 2019-01-25 2020-07-30 Biogen Ma Inc. Seed culture process for aav production
WO2020223274A1 (en) 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020168342A1 (en) * 1994-11-03 2002-11-14 Cell Genesys, Inc. Novel adenoviral vectors, packaging cell lines, recombinant adenoviruses and methods
AU764130B2 (en) * 1998-10-27 2003-08-14 Crucell Holland B.V. Improved AAV vector production
US6844192B2 (en) * 2001-06-29 2005-01-18 Wake Forest University Adenovirus E4 protein variants for virus production
WO2017180724A1 (en) * 2016-04-12 2017-10-19 Artemis Biosystems, Inc. Perfusion filtration systems
EP3456822A1 (en) * 2017-09-19 2019-03-20 CEVEC Pharmaceuticals GmbH Inducible aav rep genes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020127582A1 (en) * 1997-09-05 2002-09-12 Atkinson Edward M. Methods for generating high titer helper-free preparations of released recombinant AAV vectors
WO2020154607A1 (en) * 2019-01-25 2020-07-30 Biogen Ma Inc. Seed culture process for aav production
WO2020223274A1 (en) 2019-04-29 2020-11-05 Voyager Therapeutics, Inc. SYSTEMS AND METHODS FOR PRODUCING BACULOVIRAL INFECTED INSECT CELLS (BIICs) IN BIOREACTORS

Also Published As

Publication number Publication date
DK4107252T3 (da) 2025-03-17
JP2024144435A (ja) 2024-10-11
IL296770B1 (en) 2025-06-01
FI4107252T3 (fi) 2025-03-26
WO2022112218A1 (en) 2022-06-02
CN116209748A (zh) 2023-06-02
EP4596674A2 (en) 2025-08-06
US20240263148A1 (en) 2024-08-08
JP2023518919A (ja) 2023-05-08
KR20220164801A (ko) 2022-12-13
EP4596674A3 (en) 2025-10-22
EP4107252B1 (en) 2025-02-19
ZA202210745B (en) 2023-05-31
AU2021388811A1 (en) 2022-11-03
EP4006141A1 (en) 2022-06-01
CA3174017C (en) 2025-05-06
JP7601339B2 (ja) 2024-12-17
EP4107252A1 (en) 2022-12-28
IL296770A (en) 2022-11-01
PT4107252T (pt) 2025-03-14
CA3174017A1 (en) 2022-06-02
MX2022013385A (es) 2022-11-30
AU2021388811B2 (en) 2023-02-23
ES3017247T3 (en) 2025-05-12
IL296770B2 (en) 2025-10-01

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