KR102671030B1 - Composition for controlling rice bacterial panicle blight comprising irradiation substances of monoterpene and preparation method thereof - Google Patents

Abstract

The present invention provides a composition for controlling bacterial blight on rice grains comprising a monoterpene irradiated material as an active ingredient; Antibacterial composition; A method for controlling bacterial blight on rice germ comprising applying the control composition to plants; and a method for producing a composition for controlling bacterial rice blight, comprising the step of irradiating monoterpenes with radiation. The composition or method of the present invention can be usefully used for controlling bacterial blight on rice grains.

Description

Composition for controlling rice bacterial panicle blight comprising irradiation substances of monoterpene and preparation method thereof}

The present invention relates to a composition for controlling bacterial blight on rice grains containing a monoterpene irradiated product and a method for producing the same.

Bacterial blight or bacterial blight of rice grains is a bacterial disease that mainly occurs in rice grains caused by the bacteria Burkholderia gluma . When bacterial blight occurs on rice grains, the color changes to yellowish-white from the base of the rice grains, gradually enlarges, discolors the entire rice grains, the development of the endosperm stops, and in severe cases, it dries out. In addition, early-infected ears are pale red in color and stand upright, unable to lower their heads. Brown rice from late-infected rice grains is smaller than healthy rice and has brown stripes. When seeds infected with bacterial blight of rice germ are planted, severely diseased seeds will not grow further or will rot, and seedlings that sprouted from seeds with only a minimal degree of infection will not develop leaves or the color of the leaf sheath will change, and the growth will be poor and eventually wither and die.

Currently, it is known that there are no cultivars resistant to bacterial rice blight in Korea. Once bacterial blight on rice grains occurs, it is very difficult to control, and it interferes with the ripening of ears and reduces the quality of rice, so it must be thoroughly prevented with registered drugs before the disease occurs. The first method of controlling bacterial blight on rice grains is to use government-supplied seeds. If farmers use self-collected seeds, there is a method of carrying out a salt water screening operation in which seeds are placed in salt water and selected according to specific gravity, even if they appear to be healthy. In addition, a simple disinfection method using chlorine when disinfecting seeds is also effective in killing rice germ blight bacteria. In addition, there is a method of spraying Benasol spray after planting rice or spraying about 20 types of chemicals registered as professional chemicals, such as gas freshener and guard moisturizer, twice before and after transplanting.

However, among the above methods, the method using drugs has problems such as the risk of environmental contamination and the death of other beneficial strains. Therefore, there is an urgent need to develop an environmentally friendly control method to control bacterial rice blight.

Accordingly, the present inventors completed the present invention by confirming the effect of a monoterpene irradiation material that can control bacterial rice blight in an environmentally friendly manner.

Korean Patent No. 10-1857758 Korean Patent Publication No. 10-2015-0001241

The object of the present invention is to provide a composition for controlling bacterial blight on rice germ containing a monoterpene irradiated material as an active ingredient.

Another object of the present invention is to provide an antibacterial composition containing monoterpene irradiated material as an active ingredient.

Another object of the present invention is to provide a method for controlling bacterial blight on rice germ, which includes the step of applying the control composition to plants.

Another object of the present invention is to provide a method for producing a composition for controlling bacterial blight on rice grains, including the step of irradiating monoterpenes with radiation.

In order to achieve the above object, the present invention provides a composition for controlling bacterial blight on rice germ containing monoterpene irradiated material as an active ingredient.

Additionally, the present invention provides an antibacterial composition containing monoterpene irradiated material as an active ingredient.

In addition, the present invention provides a method for controlling bacterial blight on rice germ, comprising the step of applying the control composition to plants.

Additionally, the present invention provides a method for producing a composition for controlling bacterial blight on rice germ, comprising the step of irradiating monoterpenes with radiation.

The composition according to the present invention has significantly increased antibacterial activity against bacterial blight on rice grains, so it can be used as a replacement for chemically synthesized agents and can be used to control bacterial blight on rice grains in eco-friendly agricultural fields, thereby improving the productivity of rice. It can be usefully used to control bacterial blight on rice grains.

Figure 1 schematically illustrates a method for analyzing antibacterial activity by treating a material on a paper disk placed on the surface of a growth medium for rice blight bacteria ( Burkholderia glumae, B. glumae ).
Figure 2 shows the results of measuring the antibacterial activity against B. glumae after treatment with limonene irradiation (150-fold concentrate) (D: DMSO control group; 1: non-irradiated limonene treatment group; and 2: limonene irradiation) water treatment group).
Figure 3 shows the results of measuring the antibacterial activity against B. glumae after treating limonene irradiated products at different concentrations (5-fold, 10-fold, 50-fold, and 100-fold concentrates).
Figure 4A is the result of measuring the antibacterial activity against B. glumae after treatment with non-irradiated carvacrol, and 4B is the result of measuring the antibacterial activity against B. glumae after treatment with carvacrol irradiation. (D: DMSO control; 20: 20-fold concentrate; and 40: 40-fold concentrate).
Figure 5A is the result of measuring the antibacterial activity against B. glumae after treating non-irradiated caveol, and 5B is the result of measuring the antibacterial activity against B. glumae after treating caveol irradiated material. (D: DMSO control; 20: 20-fold concentrate; and 40: 40-fold concentrate).
Figure 6A is the result of measuring the antibacterial activity against B. glumae after treatment with non-irradiated perillyl alcohol, and 6B is the result of measuring the antibacterial activity against B. glumae after treatment with perillyl alcohol irradiation. Results (D: DMSO control; 20: 20-fold concentrate; and 40: 40-fold concentrate).

Hereinafter, the present invention will be described in detail.

The terms used in the present invention are general terms that are currently widely used as much as possible while considering the function of the present invention, but this may vary depending on the intention of a technician working in the art or the emergence of new technology. In addition, in certain cases, there are terms that are arbitrarily selected, and in this case, the meaning will be described in detail in the description of the relevant embodiment. Therefore, the terms used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than simply the name of the term.

In the present invention, when it is said to "include" a component or a step, this does not exclude other components or steps unless specifically stated to the contrary, but may further include other components or steps. it means.

The present invention provides a composition for controlling bacterial blight on rice germ containing a monoterpene irradiated material as an active ingredient.

As used herein, the term “radiation product” refers to a substance produced by irradiation of radiation. Accordingly, “monoterpene irradiated product” means a material produced from monoterpene by irradiating it.

In the present invention, the monoterpene is a terpene composed of two isoprene units and has a molecular formula of C ₁₀ H ₁₆ and may be linear or cyclic.

In the present invention, the monoterpene includes limonene, carvacrol, carveol, perillyl alcohol, carvone, alpha-terpineol, It may be selected from the group consisting of limonene epoxide and isopiperitenol, but is not limited thereto.

In the present invention, the radiation may be selected from the group consisting of X-rays, gamma rays, alpha rays, beta rays (electron rays), and neutron rays. The gamma ray may be irradiated using gamma rays emitted from radioisotopes such as cobalt (Co)-60, krypton (Kr)-85, strontium (Sr)-90, or cesium (Cs)-137.

In the present invention, the radiation may be irradiated at a dose rate of 1 to 100 kGy per hour, and preferably may be irradiated at a dose rate of 1 to 10 kGy per hour.

In the present invention, the radiation may be irradiated to an absorbed dose of 1 to 100 kGy, preferably 1 to 50 kGy, and more preferably 10 to 50 kGy. Radiation may be irradiated to an absorbed dose of 50 to 50 kGy, but is not limited thereto.

The control composition of the present invention may further include agrochemically acceptable carriers and/or excipients. The carrier may be of inorganic or organic and synthetic or natural origin, and the active composition may be mixed or formulated with this carrier to facilitate its storage, transfer and/or handling by plants, seeds, soil or other objects of treatment. It means a substance that makes something happen. In general, any substance that can be conventionally used as a carrier in pesticide, herbicide or antibacterial compositions is suitable for use in the present invention. The control composition of the present invention may be used alone or in combination with the above solid and/or liquid dispersible carrier vehicle and/or other known compatible active agents, especially other insecticides, pesticides, miticides, nematode removers, and other known compatible active agents. in the form of mixtures with plant protection agents such as fungicides, bacterial agents, rodenticides, herbicides, fertilizers, growth regulators, etc., or, if desired, in the form of preparations in specific dosages for specific applications, for example solutions, emulsions, It may also be used in ready-to-use forms such as suspensions, powders, pastes and granules. The control composition of the present invention may optionally contain a conventional inert (plant compatible or herbicidally inert) insecticidal diluent or bulking agent of the type usable in conventional insecticidal preparations or compositions, for example conventional gases, solutions. For use in emulsions, suspensions, emulsifiable concentrates, spray powders, pastes, soluble powders, dust collectors, granules, foams, pastes, tablets, aerosols, natural and synthetic substances incorporated with active compounds, microcapsules, seeds. Coating compositions for and foam cartridges, foam cans and foam coils, as well as formulations for use with burning equipment such as ULV cold air fog and warm air fog formulations can be mixed and formulated. It is desirable to adjust the carrier according to the formulation of the composition, spraying location, and spraying method.

The present invention provides a rice germ blight control product (e.g., insecticide) containing the above control composition.

The control product may be formulated in an appropriate form depending on the purpose and purpose of use, for example, granules, powders, liquids, aerosols, sprays, extracts, pastes, liquid extracts, emulsions, suspensions, capsules. It may be a liquid wetting agent, granular wetting agent, wetting agent, powder, fine granule, oil agent, gel formulation, smoke agent, fumigant, etc., and emulsion, smoke agent, fumigant or aerosol agent is most preferable as it is a formulation that can appropriately control the volatility of the pesticide composition. do.

In addition, the present invention provides a method for controlling bacterial blight on rice germ, comprising the step of applying the control composition to plants.

The composition of the present invention can be applied to plants by conventional methods. When applied to plants, the treatment can be sprayed or applied directly to the leaves, stems, branches, roots, and seeds of plants, mixed with general cultivation soil such as rice fields or fields, or seedling media or media, or applied to plants grown in water. In some cases, it can be treated on the surface of the water to control diseases. Preferably, it may be sprayed or applied directly to rice, rice seeds, or rice cultivation soil. Specific application methods are application treatment, dipping treatment, fumigation treatment or spray treatment, for example, the composition can be sprayed on soil, plant leaves, stems, seeds, flowers or fruits. In order to apply the composition of the present invention to plants, it can be diluted in water or an appropriate medium.

Additionally, the present invention provides an antibacterial composition containing monoterpene irradiated material as an active ingredient.

In the present invention, the antibacterial composition is an antibacterial composition against pathogenic microorganisms or resistant bacteria, preferably an antibacterial composition against Gram-positive bacteria or Gram-negative bacteria, and more preferably Burkholderia glumae. ) It may be an antibacterial composition having antibacterial activity against.

Additionally, the present invention provides a method for producing a composition for controlling bacterial blight on rice germ, comprising the step of irradiating monoterpenes with radiation.

In the present invention, the radiation may be selected from the group consisting of X-rays, gamma rays, alpha rays, beta rays (electron rays), and neutron rays.

In the present invention, the radiation may be irradiated at a dose rate of 1 to 100 kGy per hour, and preferably may be irradiated at a dose rate of 1 to 10 kGy per hour.

In the present invention, the radiation may be irradiated to an absorbed dose of 1 to 100 kGy, preferably 1 to 50 kGy, and more preferably 10 to 50 kGy. Radiation may be irradiated to an absorbed dose of 50 to 50 kGy, but is not limited thereto.

In the present invention, the monoterpene may be dissolved in an appropriate solvent and then irradiated with radiation. The solvent may be water, an organic solvent, or a mixture of water and an organic solvent, and the organic solvent may be methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclo It may be selected from the group consisting of hexane.

Hereinafter, the present invention will be described in more detail through examples. These examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.

Example 1. Method for producing monoterpene irradiates

Limonene, carvacrol, caveol, and perillyl alcohol (Sigma-Aldrich Co., St. Louis, MO, USA) were purchased and used with a purity of 99.0%. Each material was prepared at a concentration of 1 mM in a glass vial using ethyl acetate (JTBaker®Avantor Inc., Radnor, PA, USA) as a solvent and then irradiated. Afterwards, the irradiated material was concentrated 5 to 150 times and its antibacterial activity against rice germ blight pathogens ( Burkholderia glumae, B. glumae ) was verified. Irradiation was performed at the Co-60 gamma irradiation facility (IR-221, MDS Nordion Inc., Kanata, Canada) located at the Advanced Radiation Research Institute of the Korea Atomic Energy Research Institute (Jeongeup), with a total dose of 20 kGy at a dose rate of 5 kGy per hour at room temperature. Irradiation was performed to obtain an absorbed dose to obtain an irradiated product.

Example 2. Result of antibacterial activity analysis by limonene irradiated product

An experiment was conducted to determine whether limonene irradiated material, in which limonene, a type of monoterpene, was treated with radiation had antibacterial activity against B. glumae . Briefly, B. glumae was plated on WF-P (Wakimoto's Medium Without Potato) solid medium (2% sucrose, 05% Bacto Peptone, 005% calcium nitrate, 0082% sodium phosphate, 0005% ferrous sulfate, 15% agar). A sterilized 8 mm paper disk was placed on it. Afterwards, the limonene irradiated material was treated on a paper disk placed on the surface of the B. glumae growth medium for 48 hours, and the antibacterial activity was analyzed by measuring the length of the growth inhibition zone of B. glumae . This method is shown in Figure 1.

As a result, a growth inhibition zone of about 6 mm was confirmed for the limonene irradiated product (150-fold concentrate), while a growth inhibition zone of less than 1 mm was confirmed for the non-irradiated limonene (Figure 2). As a result, it was confirmed that the limonene irradiated product exhibited significantly increased antibacterial activity against B. glumae by about 6 times or more compared to non-irradiated limonene.

Next, an experiment was performed to analyze the antibacterial activity after treating the limonene irradiated product at different concentrations (5-fold, 10-fold, 50-fold, and 100-fold concentrate). As a result, a growth inhibition zone of approximately 1 mm was confirmed for the 10-fold concentrated limonene irradiation material, a growth inhibition zone of approximately 4 mm was confirmed for the 50-fold concentrated limonene irradiation material, and a growth inhibition zone of approximately 4 mm was confirmed for the 100-fold concentrated limonene irradiation material. A growth inhibition zone of approximately 5.5 mm was confirmed in the water (Figure 3). As a result, it was confirmed that the limonene irradiated product had antibacterial activity against B. glumae in a concentration-dependent manner.

Example 3. Results of antibacterial activity analysis by carvacrol irradiated product

An experiment was conducted to determine whether irradiated carvacrol, a type of monoterpene, treated with radiation had antibacterial activity against B. glumae . The experimental method was the same as Example 2 above.

As a result, growth inhibition zones of approximately 2 mm and 3 mm were identified for the 20- and 40-fold concentrates of carvacrol irradiation, respectively, whereas growth inhibition zones for the 20- and 40-fold concentrates of non-irradiated carvacrol were approximately 1 mm, respectively. and a growth inhibition zone of 1.5 mm was confirmed (Figure 4). As a result, it was confirmed that the carvacrol irradiated product exhibited significantly increased antibacterial activity against B. glumae by about two times or more compared to carvacrol that was not irradiated.

Example 4. Results of antibacterial activity analysis by caveol irradiated material

An experiment was conducted to determine whether caveol irradiated material, in which caveol, a type of monoterpene, was treated with radiation had antibacterial activity against B. glumae . The experimental method was the same as Example 2 above.

As a result, growth inhibition zones of approximately 2 mm and 2.2 mm were confirmed for the 20- and 40-fold concentrates of caveol irradiated material, respectively, whereas the growth inhibition zones for the 20- and 40-fold concentrates of non-irradiated caveol were approximately 1 mm, respectively. and a growth inhibition zone of 1.2 mm was confirmed (Figure 5). As a result, it was confirmed that the caveol irradiated material exhibited significantly increased antibacterial activity against B. glumae by about two times or more compared to non-irradiated caveol.

Example 5. Results of antibacterial activity analysis by perillyl alcohol irradiated product

An experiment was conducted to determine whether irradiated perillyl alcohol, a type of monoterpene, treated with radiation had antibacterial activity against B. glumae . The experimental method was the same as Example 2 above.

As a result, 20- and 40-fold concentrates of perillyl alcohol irradiation were confirmed to have growth inhibition zones of approximately 2 mm and 3 mm, respectively, while non-irradiated perillyl alcohol had growth inhibition zones of less than 1 mm. (Figure 2). As a result, it was confirmed that the perillyl alcohol irradiated product exhibited significantly increased antibacterial activity against B. glumae by about two times or more compared to non-irradiated perillyl alcohol.

Claims (15)

A composition for controlling bacterial blight on rice grains containing a monoterpene irradiated material as an active ingredient,
The composition, wherein the monoterpene is selected from the group consisting of limonene, carvacrol, carveol, and perillyl alcohol.
According to claim 1,
The composition, wherein the rice grain bacterial blight is a plant disease caused by the rice grain bacterial blight fungus ( Burkholderia glumae ).
delete According to claim 1,
The composition, wherein the radiation is selected from the group consisting of X-rays, gamma rays, alpha rays, beta rays (electron rays), and neutron rays.
According to claim 1,
The composition, wherein the radiation is irradiated at a dose rate of 1 to 10 kGy per hour.
According to claim 1,
The composition, wherein the radiation is irradiated to an absorbed dose of 10 to 50 kGy.
A method for controlling bacterial blight on rice germ comprising applying the control composition according to any one of claims 1, 2, and 4 to 6 to plants.
According to claim 7,
The method is applied to rice, rice seeds, or rice cultivation soil.
An antibacterial composition containing a monoterpene irradiated material as an active ingredient,
The composition, wherein the monoterpene is selected from the group consisting of limonene, carvacrol, carveol, and perillyl alcohol.
According to clause 9,
The composition has antibacterial activity against Burkholderia glumae .
A method for producing a composition for controlling bacterial blight on rice grains, comprising the step of irradiating monoterpenes with radiation,
A production method wherein the monoterpene is selected from the group consisting of limonene, carvacrol, carveol, and perillyl alcohol.
According to claim 11,
The method is to irradiate radiation at a dose rate of 1 to 10 kGy per hour.
According to claim 11,
The method is to irradiate the radiation to an absorbed dose of 10 to 50 kGy.
According to claim 11,
The method of dissolving the monoterpene in a solvent selected from the group consisting of water, organic solvents, and mixtures thereof and then irradiating the monoterpene with radiation.
According to claim 14,
The organic solvent is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane and cyclohexane.