KR102660992B1 - A manufacturing method of fertilizer composition for enhanced beneficial bacteria having preventing disease and growth promoting effect - Google Patents

Abstract

The beneficial bacteria active composition according to the present invention, which is effective in preventing pests and promoting growth, is manufactured by fermenting green tea, red ginseng tea, yellow tea, and ginseng tea, which are discarded in the form of tea bags after ingestion, thereby promoting the growth of crops, including saponins and ginsenosides. It contains a variety of useful nutrients, and at the same time, it can effectively prevent or improve various diseases that occur in plants.
In addition, it is eco-friendly as it does not contain any chemical fertilizers or synthetic organic pesticides, prevents water diseases, strengthens antibacterial properties, and has an excellent growth promotion effect. By adding ginkgo, which has insect repellent and pharmacological effects, as a secondary raw material, it can promote crop growth with better insect repellent and disaster prevention properties compared to existing fertilizers.

Description

{A manufacturing method of fertilizer composition for enhanced beneficial bacteria having preventing disease and growth promoting effect}

The present invention is a method of manufacturing an active composition of beneficial bacteria that is effective in preventing pests and promoting growth. It uses ginseng tops (leaves and stems) and ginseng berries, which are agricultural by-products, and ginseng tea, which is a food by-product, to create an environment conducive for bacteria to live in. It not only acts as a prebiotic, but also prevents crop diseases and strengthens antibacterial properties by inducing an increase in beneficial saponins and polyphenols through the fermentation process of by-products, and has excellent growth promotion and pest prevention effects. It relates to a method of producing a beneficial bacteria active composition.

In general, agricultural by-products such as tea bag waste and onion peels left after drinking ginseng tea, green tea, black tea, etc. are mostly collected as garbage and discarded, left on site, or landfilled to be used as fertilizer, and even the unit price or cost is reasonable. In cases where this is not done, even if there is no particular damage, it may be buried on site for economic reasons. In other words, waste is frequently discarded regardless of its condition, and even after landfill, it sometimes changes the pH or composition of the soil, adversely affecting farming.

Meanwhile, with the recent increase in the national income level, the terms 'well-being' and 'eco-friendliness' are widely used in our daily lives, exerting their power even on the agricultural products we eat. In line with this, the government is also changing its agricultural policy to eco-friendly agriculture as an alternative to opening the agricultural product market. In a sharp turn, eco-friendly agriculture has emerged as a response strategy in the era of globalization, and various policies are being promoted with eco-friendly agriculture at the center of agricultural policy.

In existing conventional farming methods, productivity could be dramatically improved by the introduction of chemical fertilizers and pesticides, but the natural laws inherent in the farmland ecosystem, which maintains vitality through organic matter, were ignored and uniform technology based on industrial logic was introduced. This resulted in one after another causing serious obstacles to maintaining the productivity of farmland, such as soil erosion, salt accumulation, and groundwater depletion and contamination.

In addition, it causes a decrease in soil strength and acidification of the soil, and suppresses microorganisms and soil biological systems, which are the final decomposers, which are the main culprits in gradually degrading farmland. Excessive use of pesticides to control pests and diseases that occur during plant growth is also a major cause. In addition to the increase in the resistance of pathogens to pesticides, the simplification and suppression of useful microorganisms in the soil leads to a rapid increase in the density of soil pathogens and harmful nematodes, resulting in a vicious cycle that requires the use of large amounts of pesticides.

To complement this, interest, development, and investment have recently been made in eco-friendly fertilizers. The use of eco-friendly fertilizers promotes recycling and resource conversion of agricultural, forestry and livestock by-products, preserves the soil environment, promotes sustainable agriculture, and promotes environmentally friendly nature. It is actively recommended as it allows for the establishment of circular agriculture and the production of high-quality and safe agricultural products.

However, these existing technologies include a method of manufacturing eco-friendly fermented fertilizer that has both crop growth promotion performance and pest disaster prevention performance by using food and beverage by-products such as ginseng tea, green tea, and black tea, or agricultural by-products such as ginseng upper part and ginseng berry. As research on this is insufficient, research on ways to supplement this is needed.

Republic of Korea Patent No. 10-1192005 (October 16, 2012)

The present invention was developed to solve the above problems, and specifically, it is specifically designed to treat food by-products, especially food and beverage by-products such as ginseng tea, green tea, and black tea, and agricultural by-products such as ginseng upper part and ginseng berry by bacteria, etc. The purpose is to provide a method for producing an active composition of beneficial bacteria that is effective in preventing pests and promoting growth, containing saponin and polyphenol, which prevents crop diseases and enhances antibacterial properties by fermenting them, and has excellent growth-promoting effects.

The present invention relates to a method for producing an active composition of beneficial bacteria that is effective in preventing pests and promoting growth.

One aspect of the present invention is,

a) A first composition is prepared by mixing 100 parts by weight of any one or more organic raw materials selected from ginseng leaves, ginseng stems, ginseng fruits, and ginseng tea with 10 to 100 parts by weight of sawdust, 10 to 200 parts by weight of sugars, and 10 to 200 parts by weight of water. manufacturing step;

b) preparing a second composition by inoculating the first composition with a bacterial strain and fermenting it at 20 to 70°C for 24 to 1,200 hours; and

c) mixing 1 to 30 parts by weight of at least one inorganic material selected from a phosphoric acid source, a calcium source, and a potassium source with 100 parts by weight of the second composition;

It relates to a method for producing an active composition of beneficial bacteria, which is effective in preventing pests and promoting growth.

In the present invention, the organic raw materials include green tea, amaranth, pear root, ginkgo, white tea, black tea, green tea, motherwort, fragrant chinensis, trifolium prickly pear, mugwort, Cheongung, white saponaria, lemon balm, chamomile, rosemary, peppermint, eucalyptus, tea tree, It may further include any one or more selected from rosehip, chamomile, rosemary, lavender, geranium, rosewood and neroli,

The bacterial strains include Bacillus megaterium , Lactobacillus plantarum , Enterococcus faecium, Acetobacter tropicalis, Acetobacter aceti , and Acetobacter. Acetobacter schutzenbachii , Acetobacter peroxidans , Acetobacter pasteurianus, Gluconobacter oxydans , Acetobacter xylinum , Komagatei Bacter laticus ( Komagataeibacter rhaeticus ), Oenococcus oeni ( Lactobacillus paracasei ), Lactobacillus reuteri ( Lactobacillus reuteri ), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus sakei ( Lactobacillus sakei ), Bacillus subtilis, Bacillus aerius , Lactococcus lactis, Enterococcus faecalis , Streptococcus thermophilus , Pedi Pediococcus pentosaceus , Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri , Lactobacillus bulgaricus , lactobacillus Bacillus helveticus , Lactobacillus rhamnosus , Lactobacillus salivarius, Leuconostoc mesenteroides , Bifidobacterium longum , Bifidobacterium It may include any one or more selected from Bifidobacterium breve , Bifidobacterium bifidum, Bifidobacterium lactis , and Bifidobacterium infantis. there is. At this time, the bacterial strain is characterized by inoculating 0.1 to 1 part by weight based on 100 parts by weight of the organic raw material.

In addition, the inorganic material is characterized in that it is at least one selected from potassium monophosphate, potassium diphosphate, potassium triphosphate, calcium carbonate, calcium oxide, calcium hydroxide, calcium phosphate, calcium chloride, and calcium sulfate.

In addition, the second composition is characterized in that it contains at least one selected from pesticide residue removers, mineral agents, nutritional additives, thickeners, surfactants, stabilizers, anti-foaming agents, anti-freezing agents, and preservatives.

The beneficial bacteria active composition according to the present invention, which is effective in preventing pests and promoting growth, is manufactured by fermenting the upper part of ginseng discarded after root collection, ginseng berries discarded due to the decline in ginseng prices, and ginseng tea discarded after consumption as tea, etc. It contains various nutrients that can promote the growth of crops, and at the same time, it can effectively prevent or improve various diseases that occur in plants.

In addition, it is eco-friendly as it does not contain any chemical fertilizers or synthetic organic pesticides, prevents water diseases, strengthens antibacterial properties, and has an excellent growth promotion effect. By adding ginkgo, which has insect repellent and pharmacological effects, as a secondary raw material, it can promote crop growth with better insect repellent and disaster prevention properties compared to existing fertilizers.

Figure 1 shows the extent of L. plantarum growth in ginseng by-products according to Examples 1 to 4 and the control group of the present invention.
Figure 2 shows the extent of L. plantarum growth in green tea by-products according to Examples 5 to 8 and the control group of the present invention.

Hereinafter, the method for producing an active composition of beneficial bacteria that is effective in preventing pests and promoting growth according to the present invention will be described in more detail using specific examples. However, the specific examples introduced below are provided as examples so that the idea of the present invention can be sufficiently conveyed to those skilled in the art.

Therefore, the present invention is not limited to the specific examples presented below and may be embodied in other forms. The specific examples presented below are only described to clarify the spirit of the present invention, and the present invention is not limited thereto.

At this time, if there is no other definition in the technical and scientific terms used, they have meanings commonly understood by those skilled in the art to which this invention pertains, and the following description will not unnecessarily obscure the gist of the present invention. Descriptions of possible notification functions and configurations are omitted.

Additionally, as used in the specification and the appended claims, the singular forms “a,” “an,” and “the” are intended to also include the plural forms, unless the context clearly dictates otherwise.

The method for producing an active composition of beneficial bacteria that is effective in preventing pests and promoting growth according to the present invention,

a) A first composition is prepared by mixing 100 parts by weight of any one or more organic raw materials selected from ginseng leaves, ginseng stems, ginseng fruits, and ginseng tea with 10 to 100 parts by weight of sawdust, 10 to 200 parts by weight of sugars, and 10 to 200 parts by weight of water. manufacturing step;

b) preparing a second composition by inoculating the first composition with a bacterial strain and fermenting it at 20 to 70°C for 24 to 1,200 hours; and

c) mixing 1 to 30 parts by weight of at least one inorganic material selected from a phosphoric acid source, a calcium source, and a potassium source with 100 parts by weight of the second composition;

may include.

In the present invention, step a) is a mixing step of the composition before fermentation, and is a step of preparing the first composition by mixing organic raw materials with sawdust, sugars, and water.

The organic raw materials are raw materials containing nitrogen or phosphoric acid, which are included in fertilizers, and may mainly include food by-products such as food and tea bags, or by-products remaining after preparing medicinal materials.

The organic raw materials may include, for example, food and beverage by-products such as ginseng tea, green tea, and black tea, ginseng upper parts such as ginseng leaves and ginseng stems, and ginseng by-products such as ginseng berries. In addition, general foods or May include fat (oil), agricultural waste, etc.

In particular, teas such as green tea, red ginseng tea, yellow tea, and ginseng tea are by-products of tea bags and contain polyphenols such as catechins and flavonols, terpenes, free amino acids, vegetable alkaloids, provitamin A, vitamin B group, vitamin C, vitamin E, and vitamins. Because it contains a large amount of vitamins such as P and minerals such as potassium, phosphorus, magnesium, zinc, and manganese, it can suppress plant diseases and supply nutrients and organic matter to the soil. Fermentation promotes low molecular weight and provides nutrients to plants for a long time. Fermented products that can supply ingredients can be manufactured.

In particular, in the case of ginseng leaves, ginseng stems, ginseng fruits, and ginseng tea made from ginseng by-products, ginseng as a raw material contains a large amount of saponins, which are pharmacologically active ingredients, or ginsenoside, a type of saponin. Therefore, it has excellent advantages in preventing or treating plant diseases. In addition, ginseng tea is made from not only the roots commonly used in oriental medicine, but also products that are not processed by farmers, such as flower stalks, leaves, stems, fruits, pulp, and seedlings of ginseng. Since these also contain a large amount of active ingredients, it is possible to supply raw materials at a low price. It is possible and has the advantage of being eco-friendly because discarded by-products can be recycled.

Saponins in ginseng are commonly called ginsenosides, a name given to them as glycosides isolated from ginseng to distinguish them from saponins of other plants. There are 30 types of ginsenosides, and they are known to have a wide variety of effects, including antioxidant activity, immunity enhancement, prevention of arteriosclerosis and high blood pressure, and anti-inflammation.

In addition, ginsenosides are contained in large quantities not only in the roots of ginseng, but also in by-products such as stems and leaves, including 4.45% in ginseng roots, 12.8 to 18.7% in ginseng leaves, 6.9% in ginseng flowers, 6.68% in ginseng fruits, and 1.6 to 1.6% in ginseng stems. It is known that ginseng leaves, flowers, and stems contain two to four times more saponin than ginseng roots, at 2.39% and 3.3% in ginseng seeds.

Ginsenosides are largely divided into protopanaxadiol-based saponins (hereinafter referred to as ‘PPD’) and protopanaxatriol-based saponins (hereinafter referred to as ‘PPT’). Both PPD and PPT structures contain a hydroxyl group (-OH group). The PPD structure contains two hydroxyl groups (-OH groups), and the PPT structure contains three hydroxyl groups (-OH groups). If hydroxyl groups are included in this way, the absorption rate into crops or the human body decreases, but when ginseng leaves, etc. are fermented with bacterial strains as in the present invention, the bacterial strains remove hydroxyl groups (-OH groups) from the PPD and PPT structures. The absorption rate into crops can be increased.

In addition to the ginseng tea, the organic raw materials include green tea, amaranth, pear, ginkgo, white tea, black tea, green tea, motherwort, fragrant chinensis, trifolium primrose, mugwort, Cheongung, white saponaria, lemon balm, chamomile, rosemary, peppermint, eucalyptus, and tea. It may further include one or more auxiliary ingredients selected from tree, rosehip, chamomile, rosemary, lavender, geranium, rosewood and neroli.

The above ingredients are added to increase the content of active ingredients such as polyphenols and free amino acids in addition to the above-mentioned pharmacologically active ingredients, and at the same time increase the antibacterial effect and insect repellent effect. In particular, any of the above ingredients such as ginkgo, amaranth, and pear. It is preferred to add one or a mixture of them.

The ginkgo ( Ginkgo biloba L. ) is a fruit of the Ginkgo family, containing small amounts of cyanic acid glycosides, gibberellins, cytokines, ginkgelic acid, hydroginkgolic acid, hydroginkgolinic acid, bilobol, ginnol, asparagine, formic acid, propionic acid, lactic acid, It contains octanoic acid, nonacosanol-10, etc. In particular, ginkgelic acid has an insect repellent effect, so when used as a fertilizer, it can suppress crop growth disruption caused by pests.

Amaranth ( Amaranthus ssp L. ) is an annual plant belonging to the Amaranth family and is called pigweed, etc., and is mainly used as a dye. In addition, it is rich in inorganic substances such as calcium, iron, and phosphorus, and organic substances such as taurine, and especially during fermentation, the polyphenol content increases. When mixed with the above-mentioned ginkgo biloba, it decomposes cyanide compounds such as ginkgo biloba and pear root, and has an antibacterial effect. It has the effect of further increasing the insect repellent effect.

The Solanum lyratum Thumb. is a vine plant of the Solanum genus of the Solanaceae family and is called by various names such as Baekmo-deung, Chokyangcheon, Baekyoung, Guimokcho, and Baekcho. The entire plant contains alkaloids such as tomatidenol, solasodine, and soladucidine, and has excellent antibacterial and insect repellent properties.

The above ingredients have antibacterial and insect repellent effects as described above, and in particular, when all of the above ingredients are mixed and used, they have the effect of further increasing the above-described pharmacological effects and the absorption rate of active ingredients in crops compared to when they are added alone.

When using a mixture of the above-mentioned ginkgo, amaranth, and baeung, the mixing ratio is not limited, but it is preferable to mix amaranth, ginkgo, and baeung at a weight ratio of 1:0.1 to 0.5:0.1 to 0.5, respectively. If it is outside the above range, the decomposition of cyan compounds such as solanine or amygdalin contained in the above-mentioned ginkgo nuts, etc. may be insufficient, which may have a negative effect on crops. In addition, when mixed with the ginseng tea, etc., it is preferable to mix 1 to 10 parts by weight of auxiliary raw materials relative to 100 parts by weight of organic raw materials to achieve the above-mentioned effects.

In addition, the organic raw materials include the pulp of fruits such as peaches, apples, kiwis, plums, watermelons, tangerines, oranges, lemons, grapefruits, quince, citrons, etc., and sources of amino acids such as rice bran, soybean meal, rice bran oil, soybean meal, cottonseed meal, perilla seed cake, etc. The same vegetable oil may additionally be included.

Additionally, the above-mentioned organic raw materials can be prepared by heat treatment at a temperature of 80 to 300 ℃ for 0.1 to 3 hours. In such pre-treated organic raw materials, microorganisms are killed through heat treatment, preventing the growth of miscellaneous bacteria during fermentation. It suppresses mechanical decomposition in tissues such as cell walls, making fermentation possible in a short period of time, and producing organic raw materials that exhibit homogeneous fermentation performance.

The sawdust is a auxiliary agent that increases the productivity and moisture absorption of fertilizer and helps organic nitrification fermentation. When sawdust is added, the fermentation rate of the composition increases and the degree of fermentation also increases, making it possible to obtain excellent quality organic fertilizer.

The material of the sawdust is not limited, but it is preferable to use sawdust made from pine trees. Pine sawdust as described above is a wood by-product and is known to aid the growth of microorganisms and have an excellent effect on plant growth. The pine tree is wood debris such as wood debris damaged at road construction sites, branches generated during logging, trees damaged by pine wilt nematodes, and pests, and can be easily mixed into the culture soil composition by converting it into sawdust.

The pine tree is a general term for trees of the cone family, and trees of the cone family include fir, Japanese fir, fir tree, spruce, German spruce, pine tree, and pine tree of the pine family. , pine trees, and pine trees, etc., and the Taxulaceae family includes metasequoia, cypress, and cedar trees, and the Cupressaceae family includes arborvitae trees, cypress trees, juniper trees, and juniper trees, which belong to the coniferous trees. It can be used as a general term for trees, etc.

In addition, the sawdust can be used by immersing it in hydrogen peroxide (H ₂ O ₂ ) washing water diluted 1,000 times for more than 10 hours, washing, and drying to remove harmful microorganisms, larvae (nematodes), eggs, etc. inside.

The amount of sawdust added is not limited, but it is preferable to add 10 to 100 parts by weight based on 100 parts by weight of the organic raw material. If the content of sawdust is less than the above range, the desired plant growth and growth rate cannot be obtained due to insufficient supply of nutrients. If it exceeds the above range, excessive decay may occur or harmful microorganisms may proliferate during the preservation process of the composition. there is.

The sugars are added to promote fermentation by the strain, and can be used regardless of the type of carbohydrate as long as they are generally soluble in water and have a sweet taste. These saccharides are divided into monosaccharides, disaccharides, and polysaccharides, and may include sugar, oligosaccharides, glucose, starch syrup, brown sugar, molasses, or mixtures thereof.

It is preferable to add 10 to 200 parts by weight of the sugars per 100 parts by weight of the organic raw material. If sugars are added below the above range, fermentation may not occur properly, and if sugars are added above the above range, harmful components may be generated due to sugar fermentation, thereby reducing the above-mentioned effects.

The water serves as a solvent to control the moisture content so that the bacterial strains described later can effectively ferment the organic raw materials. The amount added is not limited, but is mixed at a ratio of 10 to 200 parts by weight compared to 100 parts by weight of the organic fertilizer. It is desirable.

After preparing the first composition by mixing with a method such as stirring at the composition ratio described above, the first composition can be inoculated with bacterial strains and fermented at 20 to 70 ° C. for 24 to 1,200 hours to prepare the second composition. .

In the present invention, the bacterial strain is not limited to the type as long as it can ferment plants using sugars as food. Examples of such strains include Bacillus megaterium, Lactobacillus plantarum, Enterococcus faecium, Acetobacter tropicalis, and Acetobacter aceti. ), Acetobacter schutzenbachii , Acetobacter peroxidans , Acetobacter pasteurianus , Gluconobacter oxydans , Acetobacter xylinum , Komagataeibacter rhaeticus , Oenococcus oeni, Lactobacillus paracasei, Lactobacillus reuteri, Lactobacillus rhamnosus , Lactobacillus Lactobacillus sakei , Bacillus subtilis, Bacillus aerius , Lactococcus lactis, Enterococcus faecalis , Streptococcus thermophilus ), Pediococcus pentosaceus , Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus gasseri , Lactobacillus bulgaricus ), Lactobacillus helveticus, Lactobacillus rhamnosus, Lactobacillus salivarius, Leuconostoc mesenteroides , Bifidobacterium longum , Bifidobacterium breve , Bifidobacterium bifidum, Bifidobacterium lactis , and Bifidobacterium infantis , which are alone. It may be used alone or by mixing two or more.

More preferably, the bacterial strain includes Bacillus megaterium, Lactobacillus plantarum, Enterococcus faecium , or mixtures thereof.

The Bacillus megaterium is a soil rhizosphere microorganism that is high temperature and heat resistant, exhibits antifungal properties against plant pathogenic fungi, and produces endospores, which has the advantage of being useful in high temperature fermentation. In addition, because the growth rate is fast, it can effectively decompose organic matter and non-decomposable organic matter remaining in the composition in the initial high temperature fermentation stage, and has the advantage of being useful in killing thermophilic pathogenic fungi.

The Lactobacillus plantarum reduces nitric acid, uses glucose as an energy source under anaerobic conditions, and has the effect of increasing the ability to produce plant growth hormones, solubilize phosphoric acid, and antifungal ability.

Enterococcus faecium is a microorganism that can be used as a starter in the production of most dairy products such as fermented milk, butter, cheese, bread, etc., and because it adsorbs heavy metals such as lead, mercury, and cadmium in the soil, these heavy metals are absorbed into crops. It can block absorption.

In addition, the bacterial strains may further include lactic acid bacteria, yeast, photosynthetic bacteria, actinomycetes, filamentous bacteria, etc. useful for vegetation. These may be added in an appropriate amount as long as they do not impair the purpose of the invention.

The above strains can be used alone or in combination. Additionally, the amount of strains during mixing is not limited, and the method of application is also not limited. In one example, the bacterial strain is added in liquid form, and a mixed microbial powder containing Bacillus megaterium, Lactobacillus plantarium, and Enterococcus faecium in an amount of 1 × 10 ^{5 to 9} cfu/g is added to water. It is preferable to dilute it 100 to 1,000 times and add it in the form of a culture medium.

It is preferable to inoculate 0.1 to 1 part by weight of the bacterial strain based on 100 parts by weight of the organic raw material. If the inoculum amount is less than the above range, sufficient fermentation does not proceed, and if it exceeds the above range, harmful products of the strain may be generated.

In step b), the second composition can be prepared by inoculating the mixture with one or more bacterial strains and fermenting the mixture at 20 to 70°C for 24 to 1,200 hours.

Specifically, by inoculating one or more bacterial strains as described above, the above-mentioned organic raw materials can be effectively fermented and reduced to low molecular weight. Through this, crop growth promotion and disaster prevention performance can be further improved, and at the same time, the advantages of the bacterial strain, such as antifungal, phosphoric acid solubilization ability, and heavy metal adsorption, can be expressed.

Organic raw materials inoculated with the above bacterial strains include lactic acid, acetic acid, folic acid, glucuronic acid, gluconic acid, citric acid, Oxalic acid, carboxylic acid, tartaric acid, tannic acid, malonic acid, usnic acid, succinic acid, malic acid. It contains a large amount of organic acids such as acid and has an acidity of pH 1 to 6, thereby promoting the absorption of various minerals in the soil, enhancing phosphoric acid absorption by solubilizing insoluble phosphate, improving soil environment, preventing leaching of fertilizer components that are easily lost, and rooting. It can dramatically improve the efficiency and environmental friendliness of fertilizers by improving the functionality of fertilizers, such as stimulation, and suppressing the generation of ammonia gas by adjusting pH.

In addition, it contains a large amount of amino acids, which can promote crop growth, and amylase, catalase, invertase, saccharase, and rennet enzyme produced by bacteria and yeast. It contains a large amount of protease, etc. to reduce the molecular weight of ingredients in the soil so that crops can utilize them. It also contains a large amount of ingredients such as alcohol, caffeine, polyphenols, and antioxidants to promote the growth of crops and at the same time has antibacterial properties. It can demonstrate excellent disaster prevention effects.

The fermentation in step b) is preferably carried out at a temperature range of medium temperature (20 to 40°C) and high temperature (41 to 70°C) depending on the type of strain. At this time, it is recommended that the temperature order proceed from medium temperature to high temperature.

When fermentation is carried out differently depending on the temperature range as described above, growth time is provided for strains with a relatively slow growth rate, and at the same time, harmful microorganisms such as various bacteria can be killed using strains showing rapid growth. And after this is over, by gradually increasing the fermentation temperature, the problem of the introduced microorganisms not adapting to the given environment due to the temperature rise due to rapid high-temperature fermentation can be eliminated. In addition, the optimal activation temperature can be activated in harmony with each other, and physical transition occurs efficiently. In other words, the strain that grows at medium temperature leads the initial fermentation, and as the strain that grows at high temperature becomes activated, the growth of harmful strains or microorganisms can be suppressed and a killing effect can be expected along with the growth of the strain.

When fermentation is carried out at medium temperature and high temperature as described above, the holding time at each temperature range is not limited in the present invention, but is preferably carried out within the range of 12 to 600 hours for each temperature range.

The second composition, which has undergone fermentation as described above, may further be mixed with additives commonly used in fertilizers. For example, the second composition may further include one or more minerals to include potassium, calcium, and phosphorus, which are metal elements necessary for plant growth.

Calcium, potassium, and phosphorus are essential nutrients for plant growth. Phosphorus is used for energy storage and supply, potassium is used to maintain the shape of enzymes and regulates the opening and closing of pores, and calcium is used as a component of cell walls.

These minerals are not limited to the type as long as they are commonly used in the art, and examples of these minerals include potassium monophosphate (KH ₂ PO ₄ ), potassium diphosphate (K ₂ HPO ₄ ), or potassium triphosphate (K ₃ PO _{4 ).} ), metals such as potassium phosphates, calcium carbonate (CaCO ₃ ), calcium oxide (CaO), calcium hydroxide (Ca(OH) ₂ ), calcium phosphate (CaHPO ₄ ), calcium chloride (CaCl ₂ ), and calcium sulfate (CaSO ₄ ). It may include oxides, metal chlorides, etc.

In addition to the inorganic substances, the second composition may further include one or more additives selected from pesticide residue removers, minerals, nutritional additives, insect repellent, thickeners, surfactants, stabilizers, anti-foaming agents, anti-freezing agents, and preservatives.

Specifically, the residual pesticide remover is mixed with the porous ceramic powder to more effectively remove pesticide components remaining in the soil.

For example, the residual pesticide remover may be titanium dioxide (TiO ₂ ), zinc oxide (ZnO), aluminum oxide (Al ₂ O ₃ ), nickel oxide (NiO), nickel chloride (NiCl ₂ ), or a mixture thereof. , these ingredients are introduced into the soil and act as a catalyst to remove residual pesticide ingredients, and together with the porous ceramic powder, they serve to assist in more effective removal of pesticide ingredients in the soil.

The mineral agent serves to promote the growth of crops or promote root rooting by supplying mineral components, which are sufficient inorganic nutrients, to crops. Examples of these mineral agents include vermiculite, potassium chloride, potassium carbonate, Potassium nitrate, magnesium chloride, sodium citrate, iron hydroxide, iron acid, iron chloride, sodium salt, ammonium molybdenum, borax, boric acid, manganese sulfate, zinc sulfate, magnesium sulfate, zinc phosphate, manganese phosphate, ammonium phosphate, phosphate, iron phosphate, silicate or A mixture of these can be used.

The nutritional additive is supplied to the soil to promote crop growth by forming a natural organic complex that provides essential ingredients for crop growth in the soil through natural chemical and biological actions, such as humic acid and grass. Fulvic acid, guano granular, seaweed extract, or mixtures thereof can be used.

More specifically, when introduced into the soil, humic acid can form a natural organic matter complex in the soil due to natural chemical and biological actions, promote rooting, and enhance the soil's water retention capacity.

Fulvic acid can promote nutrient absorption, energy metabolism, enzyme activity, cell membrane permeability, and root rooting. In addition, as it chelates the cations contained in the soil due to its high cation substitution ability, it creates electrostatic repulsion between the soils and has the functional property of turning the soil into a sponge. In addition, it adsorbs over-applied fertilizers in the soil and slowly releases them, thereby reducing salts in the soil. It relieves salinity disturbances and exerts the effectiveness of slow-release fertilizers, promotes rooting and growth by promoting RNA synthesis of crops, and ensures deep and strong roots of crops.

In particular, when fulvic acid and humic acid are introduced into general soil at the same time, the salt in the soil neutralizes the negative charge on the surface between clay particles, eliminating the repulsive force between particles. On the other hand, when fulvic acid is added to the soil by dissociating salts and separating them from the surface of the clay particles, the clay particles become negatively charged and exert a repulsive force on each other, thereby loosening the soil structure. In other words, it eliminates soil crowding and enhances breathability and drainage.

Guano is a natural fertilizer derived from bird excrement in dry areas of South America. It contains the three main elements of nitrogen, phosphoric acid, and potassium, as well as organic matter, and also contains effective minerals such as calcium, magnesium, iron, sulfur, manganese, copper, and iodine. It can be provided to the soil.

Seaweed extract is manufactured using kelp, a natural resource of the sea, and contains a large amount of natural plant hormones and natural minerals, so it has an excellent effect in promoting crop growth. Alginic acid, a high molecular weight polysaccharide contained in large amounts, agglomerates the soil, making it breathable and drainable. Due to its nature, alginic acid, which contains a large amount of moisture, discharges when there is a lot of moisture and retains it when there is little, so it can minimize damage such as drought.

The insect repellent is intended to enhance insecticidal, insect repellent and sterilizing properties, and mainly contains a large amount of alkaloid components such as rotenone and matrine, pyrethroids such as pyrethrin, azadiractin, acidic components, and tar components such as phenolic acid. Manufactured using natural plant-based ingredients.

Examples of these insect repellent include wood vinegar, clove extract, castor oil, sumac extract, derris extract, bittersweet extract, pyrethrum extract, parsley extract, fennel root extract, sophora ginseng extract, sophora bark extract, chinensis root extract, chinensis extract, acetic acid. Or a mixture thereof can be used.

In addition, the additive may further include auxiliary additives including a thickener, surfactant, stabilizer, anti-foaming agent, anti-freezing agent, preservative, or a mixture thereof to improve physical properties such as storage stability, stability, anti-freeze, and preservability. Ingredients commonly used for manufacturing fertilizers and liquids can be introduced. At this time, the amount of each additive is not limited as long as it does not impair the purpose of the present invention. For example, the additives are preferably added at a ratio of 0.1 to 10 parts by weight relative to 100 parts by weight of the organic raw material.

The present invention may include an eco-friendly fermented fertilizer composition containing ginsenoside prepared as described above. The eco-friendly fermented fertilizer composition is manufactured by fermenting ginseng by-products such as green tea, red ginseng tea, yellow tea, and ginseng tea, which are discarded in the form of tea bags after ingestion, or ginseng leaves, ginseng stems, and ginseng fruits, which are discarded after root collection. It contains various nutrients that can promote the growth of crops, including side, and at the same time, it can effectively prevent or improve various diseases that occur in plants.

In addition, it is eco-friendly as it does not contain any chemical fertilizers or synthetic organic pesticides, prevents water diseases, strengthens antibacterial properties, and has an excellent growth promotion effect. By adding ginkgo, which has insect repellent and pharmacological effects, as a secondary raw material, it can promote crop growth with better insect repellent and disaster prevention properties compared to existing fertilizers.

Hereinafter, the present invention will be described in more detail through examples and comparative examples. However, the following examples are only specific examples for explaining preferred embodiments of the present invention, and the present invention is not limited by the following examples.

The physical properties of the specimens prepared through Examples and Comparative Examples were measured as follows.

(yield)

In order to determine the effect of application of the fermented fertilizer composition on the growth of crops, the specimens prepared through the following examples were sprayed on peppers by soil spraying, foliar fertilization, and irrigation, respectively.

Specifically, after preparing the liquid fertilizer prepared by mixing the dry fertilizer prepared through the following examples and 1,000 parts by weight of water compared to 100 parts by weight of the dry fertilizer, soil spraying was performed 20 g per 3.3 m2 of the dry fertilizer one month before planting peppers. It was evenly sprayed on the soil, and the effect on pepper cultivation was evaluated by foliar application of liquid fertilizer after planting peppers and evaluating pepper yield per unit area (kg/10a).

(Pests and pests)

In order to determine the relationship between the application of the fermented fertilizer composition and the pests and diseases of crops, the specimens prepared through the examples below were applied to peppers by soil spraying, foliar fertilization, and irrigation, respectively.

Specifically, after preparing the liquid fertilizer prepared by mixing the dry fertilizer prepared through the following examples and 1,000 parts by weight of water compared to 100 parts by weight of the dry fertilizer, soil spraying was performed 20 g per 3.3 m2 of the dry fertilizer one month before planting peppers. It was evenly sprayed on the soil, and after planting peppers, liquid fertilizer was applied to the foliage, and 30 days after spraying, the incidence rate (%) of late blight and anthracnose and the number of nematodes (aphids) present in the soil were investigated.

(Heavy metal accumulation rate)

In order to determine the effect of application of a fermented fertilizer composition on the absorption rate of heavy metals, general soil was first artificially treated with 12, 450, and 600 mg/kg of cadmium, copper, and lead, respectively, and then peppers were planted there, as in the examples, etc. The specimens prepared through were applied to peppers by soil spraying, foliar fertilization, and irrigation, respectively.

Specifically, after preparing the liquid fertilizer prepared by mixing the dry fertilizer prepared through the following examples and 1,000 parts by weight of water compared to 100 parts by weight of the dry fertilizer, soil spraying was performed 20 g per 3.3 m2 of the dry fertilizer one month before planting peppers. It was evenly sprayed on the soil, and after planting peppers, liquid fertilizer was applied to the foliage, and peppers were collected 30 days after spraying.

After the collected peppers are crushed and sieved, 1 g of the sample is placed in a 100 ml glass triangle plast, and 1 ml of concentrated sulfuric acid and 10 ml of 50% perchloric acid are added. The sample to which the decomposition solution was added was allowed to harden for more than 6 hours to be absorbed into the decomposition solution over night, and then decomposition began on a heat transfer plate in a hood device with good exhaust conditions. Initially, the temperature of the heating plate was maintained at around 80℃ for about 30 minutes, and then the temperature was gradually increased by 25℃, maintained for 45 minutes each time. When the color began to discolor to red, the temperature of the heating plate was raised to 220°C, decomposition was performed under high temperature conditions until a white sample was obtained, and the sample was naturally cooled. The cooled sample was placed on a 100 ㎖ volumetric flask with a funnel and placed on the funnel with No. 2 It was filtered using filter paper. At this time, 35 ml of lukewarm distilled water at 50°C was added to the cooled sample solution and filtered. Finally, 100 ml was collected and heavy metal analysis was performed. The analysis was measured using an inductively coupled plasma spectrophotometer (ICP: VISTA-PRO, VARIAN).

(Examples 1 to 4)

In order to check the growth of Lactobacillus plantarum, a useful strain, depending on the concentration of raw materials added, moisture was removed from the upper surface of ginseng by-products (leaves, stems, and fruits) by hot air drying at 90°C for 24 hours, and a food blender was used. It was finely ground and used as a raw material for fermentation. The possibility of cultivating microorganisms in the upper part of ginseng was evaluated.

Specifically, the culture medium was applied as a basic medium containing 0.5% yeast extract and 3% Dextrin with an industrial composition that facilitates the cultivation of L. plantarum . Ginseng upper part powder was added to the medium of the above composition to a concentration of 0.5% (Example 1), 1% (Example 2), 1.5% (Example 3), and 2% (Example 4) by weight, respectively. , sterilized at 121℃ for 15 minutes, cooled to room temperature, inoculated with the L. plantarum culture prepared the previous day at a level of 10 ⁵ CFU/ml, incubated at 37℃, 130rpm for 24 hours, plated on MRS agar by decimal dilution, and incubated at constant temperature at 37℃. Colonies formed after 48 hours of cultivation in the incubator were counted, and the number of useful strains in the culture medium was evaluated after 24 hours.

As shown in Figure 1, as a result of culturing with ginseng upper part powder, a higher growth rate was confirmed compared to the control group. As a result of repeating the experiment three times, a similar culture level was confirmed, and it was confirmed that the number of viable bacteria increased as the addition level increased.

(Examples 5 to 8)

The experiment was conducted in the same manner as in Example 1, except that green tea powder of the same concentration was used as the organic raw material.

As shown in Figure 2, as a result of the culture with green tea powder added, a higher growth rate was confirmed compared to the control group. As with the ginseng by-product, the experiment was repeated three times and a similar culture level was confirmed, and it was confirmed that the number of viable bacteria increased as the addition level increased. did.

(Example 9)

The organic raw material, which was a mixture of 40 parts by weight of dried ginseng tea leaves collected from tea bags and 20 parts by weight each of green tea leaves, red ginseng tea leaves, and yellow tea leaves, was heat-treated at a temperature of 90°C for 15 minutes to suppress the growth of various bacteria. The organic raw materials were pulverized to prepare organic fertilizer raw material powder with an average particle size of 30 mesh. And the first composition was prepared by mixing 50 parts by weight of sawdust, 50 parts by weight of molasses, and 50 parts by weight of water with 100 parts by weight of organic raw material powder.

0.2 parts by weight of Bacillus megaterium culture medium cultured at a concentration of 2 × 10 ⁷ cfu/ml was inoculated into 100 parts by weight of the prepared first composition. Then, it was put into a culture tank and fermented at 30°C for 144 hours to prepare a fermented product with a pH of 3.7.

(Example 10)

A fermented product was prepared in the same manner as in Example 1, except that the inoculating strain was Enterococcus faecium at the same concentration.

(Example 11)

A fermented product was prepared in the same manner as in Example 1, except that the inoculating strain was Lactobacillus plantarium at the same concentration.

(Example 12)

Fermented product was prepared in the same manner as in Example 2, except that 8 parts by weight of ginkgo as a secondary raw material was added based on 100 parts by weight of organic raw material powder.

(Example 13)

A fermented product was prepared in the same manner as in Example 2, except that an additional 8 parts by weight of amaranth and ginkgo mixed at a weight ratio of 1:0.3 was added based on 100 parts by weight of the organic raw material powder.

(Example 14)

Fermented product was prepared in the same manner as in Example 2, except that 8 parts by weight of amaranth, ginkgo biloba, etc. were added as additional raw materials mixed at a weight ratio of 1:0.3:0.3 based on 100 parts by weight of organic raw material powder.

[Table 1]

As shown in the table above, it can be seen that the fermented fertilizer composition according to the present invention helps the absorption of active ingredients, prevents the absorption of heavy metals, and improves crop yield by increasing resistance to pests and diseases. Specifically, Example 10, in which Enterococcus faecium was inoculated and fermented as a fermentation strain, significantly reduced the heavy metal absorption rate of crops, and it was confirmed that the crop yield was also improved accordingly.

In addition, Example 14, which added ginkgo, amaranth, and pear as additional raw materials, was confirmed to have significantly reduced the incidence of pests and diseases such as late blight, anthracnose, and nematodes compared to other examples, thereby further increasing the yield.

A detailed description of preferred embodiments of the invention disclosed above is provided to enable any person skilled in the art to make or practice the invention. Although the present invention has been described above with reference to preferred embodiments, those skilled in the art will understand that various modifications and changes can be made to the present invention without departing from the scope of the present invention. For example, a person skilled in the art may use each configuration described in the above-described embodiments by combining them with each other. Accordingly, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (6)

a) Mix 100 to 100 parts by weight of sawdust, 10 to 200 parts by weight of molasses, and 10 to 200 parts by weight of water with 100 parts by weight of any one or more organic raw materials selected from ginseng leaves, ginseng stems, ginseng fruits, and ginseng tea, and mix the organic raw materials. Preparing a first composition by further including 8 parts by weight of auxiliary raw materials mixed with amaranth, ginkgo, and pear at a weight ratio of 1:0.3:0.3, respectively, based on 100 parts by weight of the organic raw material;
b) inoculating the first composition with Enterococcus faecium as a bacterial strain and fermenting it at 20 to 70°C for 24 to 1,200 hours to prepare a second composition; and
c) mixing 1 to 30 parts by weight of at least one inorganic material selected from a phosphoric acid source, a calcium source, and a potassium source with 100 parts by weight of the second composition;
A method for producing an active composition of beneficial bacteria with excellent pest prevention and growth promotion effects on crops, comprising:
delete delete According to clause 1,
A method for producing an active composition of beneficial bacteria with excellent pest prevention and growth promotion effects on crops, characterized in that the Enterococcus faecium is inoculated in an amount of 0.1 to 1 part by weight based on 100 parts by weight of the organic raw material.
According to clause 1,
The mineral is a beneficial bacteria that is excellent in preventing pests and diseases of crops and promoting growth, characterized in that it is at least one selected from potassium monophosphate, potassium diphosphate, potassium triphosphate, calcium carbonate, calcium oxide, calcium hydroxide, calcium phosphate, calcium chloride, and calcium sulfate. Method of preparing active composition.
According to clause 1,
The second composition has a pest prevention and growth promotion effect on crops, comprising at least one selected from the group consisting of residual pesticide removers, minerals, nutritional additives, thickeners, surfactants, stabilizers, anti-foaming agents, anti-freezing agents, and preservatives. Method for producing an excellent beneficial bacterial active composition.