KR102658606B1 - Biocellulose mass manufacturing composition enforced intesil strength and biocellulose mass produced by using the same - Google Patents
Biocellulose mass manufacturing composition enforced intesil strength and biocellulose mass produced by using the same Download PDFInfo
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- 229920001340 Microbial cellulose Polymers 0.000 title claims abstract description 77
- 239000000203 mixture Substances 0.000 title claims abstract description 55
- 238000004519 manufacturing process Methods 0.000 title claims description 30
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 44
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 30
- 235000020415 coconut juice Nutrition 0.000 claims abstract description 28
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000000346 sugar Nutrition 0.000 claims abstract description 17
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 14
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 14
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 14
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 14
- 125000000896 monocarboxylic acid group Chemical group 0.000 claims abstract description 12
- 239000011734 sodium Substances 0.000 claims abstract description 12
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 244000235858 Acetobacter xylinum Species 0.000 claims description 13
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims description 13
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 13
- 235000011151 potassium sulphates Nutrition 0.000 claims description 13
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 6
- 229940116349 dibasic ammonium phosphate Drugs 0.000 claims description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 3
- 239000001177 diphosphate Substances 0.000 claims description 2
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 claims description 2
- 235000011180 diphosphates Nutrition 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- VWDWKYIASSYTQR-YTBWXGASSA-N sodium;dioxido(oxo)azanium Chemical compound [Na+].[O-][15N+]([O-])=O VWDWKYIASSYTQR-YTBWXGASSA-N 0.000 claims description 2
- 239000003206 sterilizing agent Substances 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 2
- 239000005696 Diammonium phosphate Substances 0.000 abstract 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 abstract 1
- 235000019838 diammonium phosphate Nutrition 0.000 abstract 1
- 239000011591 potassium Substances 0.000 abstract 1
- 229910052700 potassium Inorganic materials 0.000 abstract 1
- 229920002678 cellulose Polymers 0.000 description 21
- 239000001913 cellulose Substances 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 20
- 239000000463 material Substances 0.000 description 9
- 229920002749 Bacterial cellulose Polymers 0.000 description 8
- 239000005016 bacterial cellulose Substances 0.000 description 8
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 description 6
- 244000060011 Cocos nucifera Species 0.000 description 4
- 235000013162 Cocos nucifera Nutrition 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000004155 Chlorine dioxide Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000009835 boiling Methods 0.000 description 3
- 235000019398 chlorine dioxide Nutrition 0.000 description 3
- 239000000835 fiber Substances 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229920002488 Hemicellulose Polymers 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000000748 compression moulding Methods 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 229920005610 lignin Polymers 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 210000001724 microfibril Anatomy 0.000 description 2
- 239000005022 packaging material Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- VKFFEYLSKIYTSJ-UHFFFAOYSA-N tetraazanium;phosphonato phosphate Chemical compound [NH4+].[NH4+].[NH4+].[NH4+].[O-]P([O-])(=O)OP([O-])([O-])=O VKFFEYLSKIYTSJ-UHFFFAOYSA-N 0.000 description 2
- 239000000052 vinegar Substances 0.000 description 2
- 235000021419 vinegar Nutrition 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241001602876 Nata Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000009727 food gelling agent Nutrition 0.000 description 1
- 235000003086 food stabiliser Nutrition 0.000 description 1
- 235000003132 food thickener Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/02—Acetobacter
Abstract
본 발명은 인장강도가 강화된 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴에 관한 것이다.
본 발명의 바이오셀룰로오스 괴 제조용 조성물은, 코코넛 워터, 설탕, 황산암모늄((NH4)2SO4), 아세트산(CH3COOH), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4), 물로 구성되는 것이 특징이다.
본 발명에 의해, 친환경적이면서 인장강도가 강화되어 다용도로 적용가능한 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴가 제공된다.The present invention relates to a composition for producing biocellulose lumps with enhanced tensile strength and biocellulose lumps manufactured using the same.
The composition for producing biocellulose mass of the present invention includes coconut water, sugar, ammonium sulfate ((NH 4 ) 2 SO 4 ), acetic acid (CH 3 COOH), magnesium sulfate (MgSO 4 ), sodium carbonate (Na 2 CO 3 ), and sulfuric acid. It is characterized by being composed of potassium (K 2 SO 4 ), diammonium phosphate ((NH 4 ) 2 HPO 4 ), and water.
According to the present invention, a composition for producing biocellulose lumps that is environmentally friendly and has enhanced tensile strength and can be applied to various purposes, and a biocellulose lump manufactured using the same are provided.
Description
본 발명은 인장강도가 강화된 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴에 관한 것으로서, 보다 상세하게는 친환경적이면서 인장강도가 강화되어 다용도로 적용가능한 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴에 관한 것이다.The present invention relates to a composition for producing biocellulose lumps with enhanced tensile strength and biocellulose lumps manufactured using the same. More specifically, it relates to a composition for producing biocellulose lumps that is environmentally friendly and has enhanced tensile strength and can be applied to various purposes, and a composition using the same for producing biocellulose lumps with enhanced tensile strength. It relates to manufactured biocellulose lumps.
셀룰로오스는 지구상에서 가장 풍부하게 존재하는 천연고분자 중에 하나로서 대략 1011톤의 셀룰로오스가 생합성되고 있는 것으로 추산되고 있으며 대부분은 고등식물의 세포벽 섬유질 성분을 합성한다. Cellulose is one of the most abundant natural polymers on Earth, and it is estimated that approximately 10 11 tons of cellulose is biosynthesized, most of which is synthesized as cell wall fiber components of higher plants.
이러한 셀룰로오스는 구조적으로 포도당이 β-1,4 결합으로 연결된 분자량 2×105 내지 2.5×106 달톤의 다당류로서, 이론적으로는 β-1,4 결합의 절단에 의하여 구성단위인 포도당으로 분해될 수 있다. 그러나, 천연 셀룰로오스는 수소결합에 의하여 결정성 섬유(crystalline fiber)형태로 되어 있어 분자배열 상태가 치밀하고 부분적으로 결정상을 이루고 있으며, 헤미셀룰로스(hemicellulose), 펙틴(pectin), 리그닌(lignin) 등과 혼재하기 때문에, 효소의 접근이 어려워 분해속도가 느리고 분해수율이 낮아, 이를 효과적으로 이용하기 위해서는 이러한 셀룰로스의 구조를 효과적으로 분해할 수 있는 미생물 및 효소의 개발이 요구되었다.This cellulose is structurally a polysaccharide with a molecular weight of 2×10 5 to 2.5×10 6 daltons in which glucose is linked by a β-1,4 bond, and theoretically, it can be decomposed into its structural unit, glucose, by cleavage of the β-1,4 bond. You can. However, natural cellulose is in the form of crystalline fibers due to hydrogen bonding, so the molecular arrangement is dense and partially forms a crystalline phase, and it is mixed with hemicellulose, pectin, lignin, etc. Therefore, access to enzymes is difficult, the decomposition rate is slow and the decomposition yield is low, so in order to use it effectively, the development of microorganisms and enzymes that can effectively decompose the structure of cellulose was required.
이에, 1886년 초산균이 셀룰로오스를 생산한다는 사실이 보고된 이래, 미생물에 의해 생산되는 셀룰로오스(Bacterial Cellulose; 이하 'BC'라 함)는 신소재로서 끊임없는 연구 대상이 되어 왔다.Accordingly, since it was reported in 1886 that acetic acid bacteria produce cellulose, cellulose (Bacterial Cellulose (hereinafter referred to as 'BC')) produced by microorganisms has been the subject of continuous research as a new material.
특히, 미생물 유래의 셀룰로오스(BC)는 리본형 섬유(ribbin-like bundles)로 구성되는 반면, 식물 유래의 셀룰로오스는 미세섬유(microfibrils)의 묶음(bundles)형태로 구성된다.In particular, microbial-derived cellulose (BC) is composed of ribbon-like bundles, while plant-derived cellulose is composed of bundles of microfibrils.
상기 리본형 섬유(ribbin-like bundles)로 구성되는 미생물 유래의 셀룰로오스(BC)는, 미세섬유(microfibrils)의 묶음(bundles)형태로 구성되는 식물 유래의 셀룰로오스와는 달리, 리그닌이나 헤미셀룰로오스가 전혀 포함되지 않은 순수상태로 생산되며, 높은 기계적 강도와 고보습성, 고결정성, 생분해성 등의 박테리아 셀룰로오스(BC)만이 가지는 독특한 장점 때문에 다양한 산업분야에서 여러 가지 용도로 개발되고 있다.Microbial-derived cellulose (BC), which is composed of ribbon-like bundles, contains no lignin or hemicellulose, unlike plant-derived cellulose, which is composed of bundles of microfibrils. It is produced in a pure state, and is being developed for various purposes in various industrial fields due to the unique advantages of bacterial cellulose (BC), such as high mechanical strength, high moisture retention, high crystallinity, and biodegradability.
이러한 박테리아 셀룰로오스(BC)를 생산하는 대표적인 미생물로는 아세토박터 자일리늄(acetobacter xylinum)이 알려져 있으며, 이러한 아세토박토속 균주의 배양에 의해서 생산되는 고분자 물질로서, 포도당의 β-1,4 결합에 의해 생성된 중합체이다. 이러한 미생물 셀룰로오스는 생산균주의 정치배양시 배양약상에 겔상의 반투명한 펠리클(pellicle) 형태로 생성되며, 그 반투명 펠리클 내에 균체가 존재한다. Acetobacter xylinum is known as a representative microorganism that produces bacterial cellulose (BC), and is a polymer produced by culturing strains of the genus Acetobacter, which is produced by the β-1,4 bond of glucose. This is the polymer produced. Such microbial cellulose is produced in the form of a gel-like translucent pellicle on the culture medium during stationary cultivation of the production strain, and bacterial cells exist within the translucent pellicle.
이러한 미생물 셀룰로오스의 경우 코코(Nata de coco)라 불리우는 동남아시아의 전통발효식품을 포함한 각종 식이식품에 이용될 뿐만 아니라, 식품의 안정제, 겔화제 및 증점제로서 높은 효과를 발휘하여 사용되고 있다. This microbial cellulose is not only used in various dietary foods, including the traditional fermented food of Southeast Asia called coco (Nata de coco), but is also highly effective as a food stabilizer, gelling agent, and thickener.
그러나, 이러한 미생물 셀룰로오스의 경우 인장강도가 다소 낮아 빨대, 컵, 접시 등 입체형 제품가공에 사용하기 어려운 바, 친환경적으로 다양한 용도로 사용하기 어려움이 있다.However, in the case of such microbial cellulose, the tensile strength is somewhat low, making it difficult to use in the processing of three-dimensional products such as straws, cups, and plates, making it difficult to use it for various environmentally friendly purposes.
본 발명의 목적은 친환경적이면서 인장강도가 강화되어 다용도로 적용가능한 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴를 제공하는 데 있다.The purpose of the present invention is to provide a composition for producing biocellulose lumps that is environmentally friendly and has enhanced tensile strength and can be applied to various purposes, and a biocellulose lump manufactured using the same.
상기와 같은 목적을 달성하기 위한 본 발명의 바이오셀룰로오스 괴 제조용 조성물은, 코코넛 워터, 설탕, 황산암모늄((NH4)2SO4), 아세트산(CH3COOH), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4), 물로 구성되는 것이 특징이며, 보다 바람직하게 상기 조성물은 코코넛 워터 70~78중량%, 설탕 1~9중량%, 황산암모늄((NH4)2SO4) 0.1~1중량%, 아세트산(CH3COOH) 0.1~1중량%, 황산마그네슘(MgSO4) 0.001~0.1중량%, 탄산나트륨(Na2CO3) 0.001~0.1중량%, 황산칼륨(K2SO4) 0.001~0.1중량%, 제2인산암모늄((NH4)2HPO4) 0.001~0.1중량%, 물 20~28중량%로 구성되는 것이 특징이다.The composition for producing biocellulose mass of the present invention for achieving the above object includes coconut water, sugar, ammonium sulfate ((NH 4 ) 2 SO 4 ), acetic acid (CH 3 COOH), magnesium sulfate (MgSO 4 ), and sodium carbonate. (Na 2 CO 3 ), potassium sulfate (K 2 SO 4 ), ammonium diphosphate ((NH 4 ) 2 HPO 4 ), and water, and more preferably, the composition contains 70 to 78% by weight of coconut water. , sugar 1~9% by weight, ammonium sulfate ((NH 4 ) 2 SO 4 ) 0.1~1% by weight, acetic acid (CH 3 COOH) 0.1~1% by weight, magnesium sulfate (MgSO 4 ) 0.001~0.1% by weight, sodium carbonate (Na 2 CO 3 ) 0.001~0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001~0.1% by weight, dibasic ammonium phosphate ((NH 4 ) 2 HPO 4 ) 0.001~0.1% by weight, water 20~28% by weight It is characterized by being composed of %.
또 다른 본 발명인 바이오셀룰로오즈 괴의 제조방법은, 코코넛 워터를 채집하는 제1단계(S10), 상기 채집된 코코넛 워터에 물을 넣고 혼합하여 혼합물1을 제조하는 제2단계(S20), 상기 혼합물1에 설탕, 황산암모늄((NH4)2SO4), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4)을 넣고 혼합하여 혼합물 2를 제조하는 제3단계(S30), 상기 혼합물2에 아세트산(CH3COOH)를 넣고 혼합하여 바이오셀룰로오스 괴 제조용 조성물을 제조하는 제4단계(S40), 상기 바이오셀룰로오스 괴 제조용 조성물을 가열하여 살균물을 제조하는 제5단계(S50) 및, 상기 살균물에 아세토박토 자일리늄(Acetobacter xylinum) 배양물을 넣은 후, 평평한 용기에 담고 배양한 다음 이를 압축탈수하여 바이오셀룰로오즈 괴를 제조하는 제6단계(S60)를 포함하는 것이 특징이다.Another method of producing biocellulose lumps according to the present invention includes a first step of collecting coconut water (S10), a second step of mixing water into the collected coconut water and mixing to prepare mixture 1 (S20), and mixture 1 sugar, ammonium sulfate ((NH 4 ) 2 SO 4 ), magnesium sulfate (MgSO 4 ), sodium carbonate (Na 2 CO 3 ), potassium sulfate (K 2 SO 4 ), dibasic ammonium phosphate ((NH 4 ) 2 HPO 4 ), a third step of preparing mixture 2 by mixing (S30), a fourth step of preparing a composition for producing biocellulose lumps by adding acetic acid (CH 3 COOH) to the mixture 2 and mixing (S40), the biocellulose The fifth step (S50) of preparing a sterilizing product by heating the composition for producing lumps, and adding Acetobacter xylinum culture to the sterilizing material, placing it in a flat container, culturing it, and compressing and dehydrating it to produce biocellulose. It is characterized by including the sixth step (S60) of manufacturing the ingots.
또 다른 본 발명인 바이오셀룰로오즈 괴(덩어리)는 상기 제조방법에 의해 제조되는 것이 특징이다.Another feature of the present invention, biocellulose lumps, is that they are manufactured by the above manufacturing method.
본 발명이 이루고자 하는 기술적 과제들은 이상에서 언급한 기술적 과제들로 제한되지 않으며, 언급되지 않는 다른 기술적 과제들은 아래의 기재로부터 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.
본 발명에 의해, 친환경적이면서 인장강도가 강화되어 다용도로 적용가능한 바이오셀룰로오스 괴 제조용 조성물 및 이를 이용하여 제조된 바이오셀룰로오스 괴가 제공된다.According to the present invention, a composition for producing biocellulose lumps that is environmentally friendly and has enhanced tensile strength and can be applied to various purposes, and a biocellulose lump manufactured using the same are provided.
도 1은 본 발명의 실시예1에 따른 바이오셀룰로오스 괴(덩어리) 제조방법을 나타낸 제조공정도이다.
도 2는 평평한 용기에 바이오셀룰로오스 괴 제조용 조성물을 넣고 배양한 후 배양이 완료된 괴(덩어리) 상태를 나타낸 사진이다.
도 3은 상기 도 2의 배양이 완료된 괴(덩어리)를 용기에서 빼낸 사진이다.Figure 1 is a manufacturing process diagram showing a method for producing biocellulose lumps according to Example 1 of the present invention.
Figure 2 is a photograph showing the state of a lump upon completion of culture after placing a composition for producing a biocellulose lump in a flat container and culturing it.
Figure 3 is a photograph of the lump (lump) in which the culture of Figure 2 has been completed is removed from the container.
이하, 첨부된 도면을 참조하여 본 발명의 바람직한 실시예 및 실험예를 상세하게 설명하며, 본 발명의 요지를 불필요하게 흐릴 수 있는 공지 기능 및 구성에 대한 상세한 설명은 생략한다.Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the attached drawings, and detailed descriptions of known functions and configurations that may unnecessarily obscure the gist of the present invention will be omitted.
본 발명의 발명자들은 기존의 바이오셀룰로오스 괴(덩어리)의 단점인 인장강도가 약한점을 보완하기 위해 여러 연구를 시행한 결과, 바이오셀룰로오스 괴 제조용 조성물이 코코넛 워터, 설탕, 황산암모늄((NH4)2SO4), 아세트산(CH3COOH), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4), 물로 구성될 경우 인장강도가 높게 형성되어 다양한 용도로 사용가능함을 확인한 것으로써, 본 발명을 완성하게 되었다.The inventors of the present invention conducted several studies to compensate for the weak tensile strength of existing biocellulose lumps, and as a result, the composition for producing biocellulose lumps was found to contain coconut water, sugar, and ammonium sulfate ((NH 4 ) 2 SO 4 ), acetic acid (CH 3 COOH), magnesium sulfate (MgSO 4 ), sodium carbonate (Na 2 CO 3 ), potassium sulfate (K 2 SO 4 ), ammonium dibasic phosphate ((NH 4 ) 2 HPO 4 ), The present invention was completed by confirming that when composed of water, the tensile strength is high and it can be used for various purposes.
이때, 더욱 중요한 것은 상기 조성물의 구성상태인 것으로, 보다 바람직하게 상기 조성물은 코코넛 워터 70~78중량%, 설탕 1~9중량%, 황산암모늄((NH4)2SO4) 0.1~1중량%, 아세트산(CH3COOH) 0.1~1중량%, 황산마그네슘(MgSO4) 0.001~0.1중량%, 탄산나트륨(Na2CO3) 0.001~0.1중량%, 황산칼륨(K2SO4) 0.001~0.1중량%, 제2인산암모늄((NH4)2HPO4) 0.001~0.1중량%, 물 20~28중량%로 구성되는 것이 특징이다.At this time, what is more important is the composition of the composition, and more preferably, the composition contains 70 to 78% by weight of coconut water, 1 to 9% by weight of sugar, and 0.1 to 1% by weight of ammonium sulfate ((NH 4 ) 2 SO 4 ). , acetic acid (CH 3 COOH) 0.1 to 1% by weight, magnesium sulfate (MgSO 4 ) 0.001 to 0.1% by weight, sodium carbonate (Na 2 CO 3 ) 0.001 to 0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001 to 0.1 weight. %, dibasic ammonium phosphate ((NH 4 ) 2 HPO 4 ) 0.001 to 0.1% by weight, and 20 to 28% by weight of water.
다시 말해, 상기 조성물은 아세토박토 자일리늄(Acetobacter xylinum) 균주로부터 인장강도가 높은 바이오셀룰로오스 생산하기 위한 영양성분인 것으로, 상기 코코넛 워터는 바이오셀룰로오스 괴(덩어리)의 생성에 필요한 질소원, 탄소원 및 섬유소가 풍부한 것을 특징으로 조성물 전체중량을 기준으로 70~78 중량%로 함유되는 것이 특징이다. In other words, the composition is a nutrient for producing biocellulose with high tensile strength from Acetobacter xylinum strain, and the coconut water contains nitrogen source, carbon source, and fiber necessary for the production of biocellulose lumps. It is characterized by being abundant and containing 70 to 78% by weight based on the total weight of the composition.
이때, 상기 코코넛 워터의 함량이 70 중량% 미만으로 함유될 경우 영양성분의 함량이 낮아 바이오셀룰로오스 생산량이 적어 본 발명이 목적하는 효과를 얻기 어려우며, 78 중량%를 초과할경우 다른 재료들과의 배합비율이 맞지 않아 이 역시 바이오셀룰로오스 생산량에 악영향을 미치게 된다. At this time, if the content of the coconut water is less than 70% by weight, the content of nutrients is low and biocellulose production is low, making it difficult to obtain the desired effect of the present invention, and if it exceeds 78% by weight, it is difficult to obtain the desired effect of the present invention. If the ratio is not right, this will also have a negative impact on biocellulose production.
상기 설탕은 바이오셀룰로오스 괴(덩어리)의 생성에 필요한 탄소원을 보완해주어 아세토박토 자일리늄(Acetobacter xylinum) 균주로부터 바이오셀룰로오스의 생성능을 높이기 위해서 함유되는 성분으로 조성물 전체중량을 기준으로 1~9 중량%로 함유되는 것이 특징이다. The sugar is an ingredient contained to increase the ability to produce biocellulose from Acetobacter xylinum strains by supplementing the carbon source necessary for the production of biocellulose lumps, and is contained in 1 to 9% by weight based on the total weight of the composition. It is characterized by its inclusion.
이때, 상기 설탕의 함량이 1 중량% 미만으로 함유될 경우 상승된 바이오셀룰로오스 생성능을 얻기 어려우며, 9 중량%를 초과하여 함유될 경우 오히려 아세토박토 자일리늄(Acetobacter xylinum) 균주의 성장을 더디게 함에 따라 인장강도가 낮게 형성되어 본 발명의 목적을 이루기 어렵게 된다. At this time, if the sugar content is less than 1% by weight, it is difficult to obtain increased biocellulose production ability, and if the sugar content is more than 9% by weight, it actually slows down the growth of the Acetobacter xylinum strain, resulting in increased biocellulose production. The strength is formed to be low, making it difficult to achieve the purpose of the present invention.
이외, 상기 조성물에는 황산암모늄((NH4)2SO4) 0.1~1중량%, 아세트산(CH3COOH) 0.1~1중량%, 황산마그네슘(MgSO4) 0.001~0.1중량%, 탄산나트륨(Na2CO3) 0.001~0.1중량%, 황산칼륨(K2SO4) 0.001~0.1중량%, 제2인산암모늄((NH4)2HPO4) 0.001~0.1중량%, 물 20~28중량%가 함유되는 것을 특징으로, 이러한 상기 조성성분들은 인장강도가 높은 바이오셀룰로오스를 생성하기 위한 필수 조건인 것으로 상기 중량 범위를 벗어날 경우 오히려 혼합비율이 맞지 않아 본 발명이 목적을 이루기 어렵게 된다.In addition, the composition includes ammonium sulfate ((NH 4 ) 2 SO 4 ) 0.1 to 1% by weight, acetic acid (CH 3 COOH) 0.1 to 1% by weight, magnesium sulfate (MgSO 4 ) 0.001 to 0.1% by weight, and sodium carbonate (Na 2 Contains CO 3 ) 0.001~0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001~0.1% by weight, dibasic ammonium phosphate ((NH 4 ) 2 HPO 4 ) 0.001~0.1% by weight, and water 20~28% by weight. Characteristically, these components are essential conditions for producing biocellulose with high tensile strength, and if they are outside the above weight range, the mixing ratio does not match, making it difficult to achieve the purpose of the present invention.
이하, 도 1에 도시된 바를 토대로 상기와 같이 구성된 조성물을 이용한 바이오셀룰로오스 괴(덩어리) 제조공정에 관하여 구체적으로 설명하면 다음과 같다. Hereinafter, based on what is shown in FIG. 1, the process for manufacturing biocellulose lumps using the composition configured as above will be described in detail as follows.
<본 발명의 바이오셀룰로오스 괴(덩어리)의 제조공정><Manufacturing process of biocellulose lump of the present invention>
1. 제1단계 : 코코넛 워터 채집단계(S10)1. Step 1: Coconut water collection step (S10)
본 단계에서는 코코넛 워터를 채집하는 것이 특징이다.This step is characterized by collecting coconut water.
설명하면, 코코넛 워터(coconut water)는 코코넛 안에 든 투명한 액체를 나타내는 것이다. To explain, coconut water refers to the clear liquid inside a coconut.
이에, 본 단계에서는 생 코코넛을 가른 후 코코넛 안에 함유되어 있는 코코넛 워터를 채집한 후, 이를 여과하여 코코넛 워터를 채집한다.Therefore, in this step, the raw coconut is split, the coconut water contained in the coconut is collected, and then the coconut water is collected by filtering it.
이러한, 코코넛 워터는 기존 바이오셀룰로오스 생산소재로 흔히 사용되고 있는 소재이긴 하나, 기존에는 마스크팩의 용도로 사용되는 것으로 영양성분의 흡수율이 높을 뿐 인장강도는 여전이 낮은 단점을 갖고 있다. Although coconut water is a commonly used material for producing existing biocellulose, it has the disadvantage of having a high absorption rate of nutrients as it is used for mask packs, but its tensile strength is still low.
2. 제2단계 : 혼합물1 제조단계(S20)2. Step 2: Mixture 1 manufacturing step (S20)
본 단계에서는 상기 채집된 코코넛 워터에 물을 넣고 혼합하여 혼합물1을 제조하는 것이 특징이다. In this step, mixture 1 is prepared by adding water to the collected coconut water and mixing it.
설명하면, 상기 채집된 코코넛 워터에 하기 여러 재료들의 혼합용이성을 높이기 위해 먼저 채집된 코코넛 워터에 일정량의 물을 넣고 혼합하여 혼합물 1을 제조하는 것이 바람직하다. To explain, in order to increase the ease of mixing the various ingredients below with the collected coconut water, it is preferable to first prepare Mixture 1 by adding a certain amount of water to the collected coconut water and mixing it.
이때, 상기 코코넛 워터와 상기 물은 2~3 : 1 중량비로 혼합하는 것을 특징으로, 이는 하기 재료들의 혼합용이성 및 높은 인장강도를 갖는 바이오셀룰로오스를 생산하기 위한 것이다. At this time, the coconut water and the water are mixed at a weight ratio of 2 to 3: 1, in order to produce biocellulose with high tensile strength and ease of mixing of the following materials.
3. 제3단계 : 혼합물2 제조단계(S30)3. Step 3: Mixture 2 manufacturing step (S30)
본 단계에서는 상기 제2단계의 상기 혼합물1에 아세토박토 자일리늄(Acetobacter xylinum) 균주의 바이오셀룰로오즈 생산능을 높여주는 역할을 하는 설탕, 황산암모늄((NH4)2SO4), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4)을 넣고 혼합하여 혼합물 2를 제조하는 것이 특징이다. In this step, sugar, ammonium sulfate ((NH 4 ) 2 SO 4 ), and magnesium sulfate (MgSO), which play a role in increasing the biocellulose production ability of the Acetobacter xylinum strain, are added to the mixture 1 of the second step. 4 ), sodium carbonate (Na 2 CO 3 ), potassium sulfate (K 2 SO 4 ), and ammonium diphosphate ((NH 4 ) 2 HPO 4 ) are added and mixed to prepare mixture 2.
4. 제4단계 : 바이오셀룰로오스 괴 제조용 조성물 제조단계(S40)4. Step 4: Manufacturing step of composition for producing biocellulose lump (S40)
본 단계에서는 인장강도가 높은 바이오셀룰로오스 괴(덩어리)를 제조하기 위해, 상기 제3단계의 상기 혼합물2에 아세트산(CH3COOH)를 넣고 바이오셀룰로오스 괴 제조용 조성물을 제조하는 것이 특징이다. In this step, in order to produce a biocellulose lump with high tensile strength, acetic acid (CH 3 COOH) is added to the mixture 2 of the third step to prepare a composition for producing a biocellulose lump.
이렇게 제조된 상기 바이오셀룰로오스 괴 제조용 조성물은 보다 바람직하게는 코코넛 워터 70~78중량%, 설탕 1~9중량%, 황산암모늄((NH4)2SO4) 0.1~1중량%, 아세트산(CH3COOH) 0.1~1중량%, 황산마그네슘(MgSO4) 0.001~0.1중량%, 탄산나트륨(Na2CO3) 0.001~0.1중량%, 황산칼륨(K2SO4) 0.001~0.1중량%, 제2인산암모늄((NH4)2HPO4) 0.001~0.1중량%, 물 20~28중량%로 구성되는 것이 특징인 것으로 , 상기 중량범위를 이룰경우 높은 인장강도를 갖는 괴(덩어리)를 제조할 수 있게 되며, 이를 통해 다양한 용도로 적용가능하게 되는 것이다.The composition for producing biocellulose mass prepared in this way more preferably contains 70 to 78% by weight of coconut water, 1 to 9% by weight of sugar, 0.1 to 1% by weight of ammonium sulfate ((NH 4 ) 2 SO 4 ), and acetic acid (CH 3 COOH) 0.1~1% by weight, magnesium sulfate (MgSO 4 ) 0.001~0.1% by weight, sodium carbonate (Na 2 CO 3 ) 0.001~0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001~0.1% by weight, diphosphate It is characterized by being composed of 0.001 to 0.1% by weight of ammonium ((NH 4 ) 2 HPO 4 ) and 20 to 28% by weight of water. When the above weight range is achieved, it is possible to manufacture lumps with high tensile strength. And through this, it can be applied for various purposes.
5. 제5단계 : 살균물 제조단계(S50)5. Step 5: Sterilization product manufacturing step (S50)
본 단계에서는 상기 제4단계의 바이오셀룰로오스 괴 제조용 조성물을 끓여 살균물을 제조하는 것이 특징이다.This step is characterized by producing a sterilizing product by boiling the composition for producing biocellulose lumps in the fourth step.
설명하면, 하기 아세토박토 자일리늄(Acetobacter xylinum) 균주의 바이오셀룰로오즈 생산능을 높이기 위해서는 상기 조성물은 균이 없는 상태로 유지되어야 한다. 이에, 상기 조성물을 100℃가 될때까지 끓여서 살균하여 살균물 형태로 제조한 후, 이를 자연상태로 서서히 상온까지 식혀 사용한다.To explain, in order to increase the biocellulose production ability of the Acetobacter xylinum strain described below, the composition must be maintained in a bacteria-free state. Accordingly, the composition is prepared in the form of a sterilized product by boiling and sterilizing it until it reaches 100°C, and then it is gradually cooled to room temperature in its natural state before use.
6. 제6단계 : 바이오셀룰로오즈 괴(덩어리) 제조단계(S60)6. Step 6: Biocellulose lump manufacturing step (S60)
본 단계에서는 상기 제5단계의 살균물에 아세토박토 자일리늄(Acetobacter xylinum) 배양물을 넣은 후, 평평한 용기에 담고 배양한 다음 이를 압축탈수하여 바이오셀룰로오즈 괴(덩어리)를 제조하는 것이 특징이다. In this step, the Acetobacter xylinum culture is added to the sterilization material of the fifth step, placed in a flat container, cultured, and then compressed and dehydrated to produce biocellulose lumps.
이때, 사용된 아세토박토 자일리늄(Acetobacter xylinum) 균주는 한국생명공학연구소, 한국미생물보존센터등으로부터 누구나 용이하게 분양받을 수 있다.At this time, the Acetobacter xylinum strain used can be easily obtained by anyone from the Korea Research Institute of Bioscience and Biotechnology, Korea Microorganism Conservation Center, etc.
상기 배양물은 균주를 배양한 배지 내에 포함되어 있는 모든 물질을 포함하는 물질을 의미할 수 있으며, 예컨대 균주 배양의 결과물인 대사물 또는 분비물을 포함하는 물질 또는 그 파쇄물을 의미할 수 있고 균주 자체도 배양물 내에 포함되어 있을 수 있다. 또한 상기 배양액은 당업계에 통상적으로 이용되는 방법에 따라 배양할 수 있다.The culture may refer to a material containing all materials contained in the medium in which the strain is cultured, for example, a material containing metabolites or secretions resulting from strain culture, or a fragment thereof, and the strain itself. It may be contained within the culture. Additionally, the culture medium can be cultured according to methods commonly used in the art.
또한, 상기 배양물로는 1×107 CFU/ml 이상 농도의 미생물 배양물을 준비하는 것이 가장 적합하며, 이러한 상기 아세토박토 자일리늄(Acetobacter xylinum) 배양물은 상기 살균물 1L당 40~50 ml를 넣는 것이 가장 좋다. In addition, it is most suitable to prepare a microbial culture with a concentration of 1 × 10 7 CFU / ml or more as the culture, and the Acetobacter xylinum culture is 40 to 50 ml per 1 L of the sterilant. It is best to put .
그 다음 이를 정치배양하기 위해 평평한 용기에 담고 배양하는 것으로 보다 바람직하게는 상기 용기를 천으로 감싼 후, 이를 25~30℃에서 2~3일간 1차 배양한 다음, 천을 제거한 후 25~30℃에서 4~6일간 배양하는 것이 좋다. Then, for stationary culture, it is placed in a flat container and cultured. More preferably, the container is wrapped with a cloth, and it is first cultured at 25-30°C for 2-3 days, and then the cloth is removed and cultured at 25-30°C. It is recommended to incubate for 4 to 6 days.
이는 인장강도가 높은 괴(덩어리)를 제조하기 위한 필수조건인 것으로 이를 통해 빨대, 컵, 접시 등 입체형 제품가공에 사용가능한 바이오셀룰로오즈 괴(덩어리)를 제조할 수 있게 된다.This is an essential condition for manufacturing lumps with high tensile strength, which makes it possible to manufacture biocellulose lumps that can be used in the processing of three-dimensional products such as straws, cups, and plates.
이렇게 배양한 후 생성된 셀룰로오즈를 용기에서 분리하여 위, 아래면을 세척한 후 40~50시간동안 압축탈수하여 바이오셀룰로오즈 괴(덩어리)를 제조한다.After culturing in this way, the produced cellulose is separated from the container, washed on the top and bottom, and then compressed and dehydrated for 40 to 50 hours to produce biocellulose lumps.
이렇게 제조된 바이오셀룰로오즈 괴(덩어리)는 보관율을 높이기 위해 포장재에 살균액과 함께 넣은 후 진공포장하여 보관한다.To increase the storage rate, the biocellulose lumps produced in this way are placed in packaging material with a sterilizing solution and then vacuum-packed for storage.
이때, 상기 살균액은 물, 식초, 이산화염소로 구성된 것으로 보다 바람직하게는 셀룰로오즈 1kg당 물 10~20 ml, 식초 10~20 ml, 이산화염소 0.1~0.2g으로 구성된 살균액을 사용하는 것이 가장 좋다.At this time, the sterilizing solution consists of water, vinegar, and chlorine dioxide, and more preferably, it is best to use a sterilizing solution consisting of 10 to 20 ml of water, 10 to 20 ml of vinegar, and 0.1 to 0.2 g of chlorine dioxide per 1 kg of cellulose. .
이하에서는 실시예 및 실험예를 들어 본 발명에 관하여 더욱 상세하게 설명할 것이나, 이들 실시예 및 실험예는 단지 설명의 목적을 위한 것으로 본 발명의 보호 범위를 제한하고자 하는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples and experimental examples. However, these examples and experimental examples are for illustrative purposes only and are not intended to limit the scope of protection of the present invention.
<실시예 1> 바이오셀룰로오스 괴(덩어리)1 제조<Example 1> Production of biocellulose lump (lump) 1
생 코코넛을 가른 후 코코넛 워터를 채집한 다음 망을 통해 걸러내어 600L의 코코넛 워터를 채집하였다. After splitting the raw coconut, the coconut water was collected and then filtered through a mesh to collect 600L of coconut water.
그 다음 상기 600L의 코코넛 워터에 물 200L를 넣은 후 잘 섞이도록 저어 혼합하여 혼합물 1을 제조하였다. Next, 200L of water was added to the 600L of coconut water and stirred to mix well to prepare Mixture 1.
그 다음 상기 혼합물 1에 설탕 16.7kg, 황산암모늄 8kg, 황산마그네슘 67g, 탄산나트륨 67g, 황산칼륨 67g, 제2인산암모늄 67g을 넣고 잘 섞이도록 저어 혼합하여 혼합물 2를 제조하였다. Next, 16.7 kg of sugar, 8 kg of ammonium sulfate, 67 g of magnesium sulfate, 67 g of sodium carbonate, 67 g of potassium sulfate, and 67 g of ammonium dibasic were added to mixture 1 and stirred to mix well to prepare mixture 2.
그 다음 상기 혼합물 2에 아세트산 2L를 넣고 잘 섞이도록 저어 혼합하여 바이오셀룰로오스 괴 제조용 조성물을 제조하였다. Then, 2L of acetic acid was added to the mixture 2 and stirred to mix well to prepare a composition for producing biocellulose lumps.
그 다음 상기 조성물을 살균을 목적으로 100℃가 될 때까지 끓여서 살균한 후 자연상태로 서서히 상온까지 식혀 살균물을 제조하였다. Next, for the purpose of sterilization, the composition was sterilized by boiling it until it reached 100°C, and then slowly cooled to room temperature in its natural state to prepare a sterilizing product.
그 다음 멸균된 Tray에 상기 살균물을 1리터씩 소분하여 담고, Acetobacter xylinum을 배양한 용액을 50ml씩 넣어주었다. Next, 1 liter of the above-mentioned sterilizing material was placed in small portions in sterilized trays, and 50 ml of Acetobacter xylinum culture solution was added thereto.
그 다음 용액이 담긴 Tray에 신문지로 덮개를 만들어 덮고, Tray를 층층이 쌓은 후, 층층이 쌓인 Tray 전체를 천으로 2일간 감싸두었다.(실온 보관, 28℃이상)Next, the tray containing the solution was covered with newspaper, the trays were stacked in layers, and the entire tray was wrapped with cloth for 2 days (stored at room temperature, above 28°C).
그 다음 2일 후 Tray를 덮고 있는 천을 제거 후 5일동안 실온(28℃ 이상)보관 한 후, 5일이 지난 후 Tray에서 Cellulose를 분리하여 위, 아래면을 세척하였다. Then, after 2 days, the cloth covering the tray was removed and stored at room temperature (above 28°C) for 5 days. After 5 days, the cellulose was separated from the tray and the top and bottom sides were washed.
그 다음 세척된 Cellulose 를 48시간 동안 압축 탈수하고, 이 압축 탈수된 Cellulose 1Kg을 포장재에 넣고 살균액 (Cellulose 1Kg, 1Pack 당/ 물 20ml, 식초 (Acid acetic) 20ml, 이산화염소 0.2g)을 넣은 후 진공 포장하여 바이오셀룰로오스 괴(덩어리)1을 제조하였다. Next, the washed Cellulose is compressed and dehydrated for 48 hours, and 1Kg of this compressed and dehydrated Cellulose is placed in a packaging material and a sterilizing solution (1Kg of Cellulose, 20ml of water per 1 pack, 20ml of Acid acetic, 0.2g of chlorine dioxide) is added. Biocellulose lump 1 was prepared by vacuum packaging.
<비교예 1 내지 5> 바이오셀룰로오스 괴(덩어리)2 내지 6 제조<Comparative Examples 1 to 5> Production of biocellulose lumps 2 to 6
(L)coconut water
(L)
(kg)sugar
(kg)
(kg)ammonium sulfate
(kg)
(L)acetic acid
(L)
(kg)Magnesium sulfate
(kg)
(kg)sodium carbonate
(kg)
(kg)potassium sulfate
(kg)
(kg)Ammonium dibasic phosphate
(kg)
(L)water
(L)
상기 실시예1과 같은 방법으로 제조하되, 조성물의 함량비율을 상기 표 1의 조성대로 적용하여 바이오셀룰로오스 괴(덩어리)2 내지 6을 제조하였다.Biocellulose lumps 2 to 6 were prepared in the same manner as in Example 1, but the content ratio of the composition was applied according to the composition in Table 1 above.
<비교예 6> 바이오셀룰로오스 괴(덩어리)7 제조<Comparative Example 6> Production of biocellulose lump (lump) 7
상기 실시예1과 같은 방법으로 제조하되, 멸균된 Tray에 상기 살균물을 1리터씩 소분하여 담고, Acetobacter xylinum을 배양한 용액을 50ml씩 넣은 후, 용액이 담긴 Tray에 신문지로 덮개를 만들어 덮고, Tray를 층층이 쌓은 후, 층층이 쌓인 Tray 전체를 7일간 실온 보관(28℃이상)하여 바이오셀룰로오스 괴(덩어리)7을 제조하였다. Prepared in the same manner as Example 1, except that 1 liter of the sterilizing agent was placed in sterilized trays, 50 ml of Acetobacter xylinum cultured solution was added, and then the tray containing the solution was covered with newspaper, After stacking the trays in layers, the entire stacked trays were stored at room temperature (above 28°C) for 7 days to produce biocellulose lumps7.
<실험예 1> 바이오셀룰로오스 괴(덩어리)들의 굴곡탄성률 및 인장강도 확인<Experimental Example 1> Confirmation of flexural modulus and tensile strength of biocellulose lumps
상기 실시예 1, 비교예 1 내지 6에서 생산된 바이오셀룰로오스 괴(덩어리)들을 대상으로 굴곡탄성률 및 인장강도를 측정하였다. The flexural modulus and tensile strength of the biocellulose lumps produced in Example 1 and Comparative Examples 1 to 6 were measured.
이때, 굴곡탄성률(Flexural Modulus, FM)은 각 시료들을 각각의 규격에 따라 200℃에서 압축성형(compression molding)하여 시편을 5개씩 제조한 후, 만능시험기를 사용하여 상온에서 ASTM D790에 따라 굴곡탄성률을 측정하고 평균값을 구하였다.At this time, the flexural modulus (FM) was determined by compression molding each sample at 200°C according to each standard to produce five specimens, and then measuring the flexural modulus according to ASTM D790 at room temperature using a universal testing machine. was measured and the average value was obtained.
인장강도(Tensile Strength, TS)는 각 시료들을 각각의 규격에 따라 200℃에서 압축성형하여 시편을 5개씩 제조한 후, 만능시험기를 사용하여 상온에서 ASTM D638에 따라 인장강도를 측정하고 평균값을 구하였다.Tensile Strength (TS) is obtained by compression molding each sample at 200°C according to each standard to produce five specimens, then measuring the tensile strength at room temperature according to ASTM D638 using a universal testing machine and calculating the average value. did.
그 결과 하기 표 2와 같이 나타났다. The results were shown in Table 2 below.
(MPa)tensile strength
(MPa)
상기 표 2에 나타나 있듯이 실시예 1의 바이오셀룰로오스 괴(덩어리)1은 인장강도 및 굴곡탄성률이 가장 높게 나타나는 반면, 비교예1의 바이오셀룰로오스 괴(덩어리)2는 수분의 함량이 너무 많아 흐물거리는 물성을 나타내는 바, 인장강도 및 굴곡탄성률이 실시예1 보다 낮게 나타났으며 비교예 2의 바이오셀룰로오스 괴(덩어리) 3은 너무 딱딱하여 인장강도 및 굴곡탄성률이 낮게 나타남을 확인하였다.As shown in Table 2, the biocellulose lump 1 of Example 1 showed the highest tensile strength and flexural modulus, while the biocellulose lump 2 of Comparative Example 1 had too much moisture and had mushy physical properties. As shown, the tensile strength and flexural modulus were lower than those of Example 1, and the biocellulose lump 3 of Comparative Example 2 was confirmed to be too hard, resulting in low tensile strength and flexural modulus.
또한, 비교예 3의 바이오셀룰로오스 괴(덩어리)3에서는 당분의 함량이 적어 셀룰로오스 형성이 적어 인장강도 및 굴곡탄성률이 낮게 나타났으며, 비교예 4의 바이오셀룰로오스 괴(덩어리)4는 경화정도가 높아 너무 딱딱하여 인장강도 및 굴곡탄성률이 낮게 나타남을 확인하였다.In addition, biocellulose lump 3 of Comparative Example 3 had a low sugar content and low cellulose formation, resulting in low tensile strength and flexural modulus, while biocellulose lump 4 of Comparative Example 4 had a high degree of hardening. It was confirmed that the tensile strength and flexural modulus were low because it was too hard.
또한, 비교예 5의 바이오셀룰로오스 괴(덩어리)5는 배지조건이 맞지 않아 이 역시 셀룰로오스 형성이 적어 인장강도 및 굴곡탄성률이 낮게 나타났으며, 비교예 6의 바이오셀룰로오스 괴(덩어리)6은 배양조건이 맞지 않아 이 역시 셀룰로오스 형성이 적어 인장강도 및 굴곡탄성률이 낮게 나타남을 확인하였다.In addition, biocellulose lump 5 in Comparative Example 5 did not meet the medium conditions, so it also showed low cellulose formation, resulting in low tensile strength and flexural modulus, and biocellulose lump 6 in Comparative Example 6 showed low culture conditions. As this was not correct, it was confirmed that the tensile strength and flexural modulus were low due to low cellulose formation.
이와 같이 상기 본 발명인 실시예 1의 바이오셀룰로오스 괴(덩어리)1의 경우 인장강도 및 굴곡탄성률이 매우 높은 바, 이는 기계적 특성을 나타내는 것으로 이를 통해 컵, 빨대, 접시 등 입체적 상품성형까지 적용가능함을 예측할 수 있다.In this way, in the case of the biocellulose lump 1 of Example 1 of the present invention, the tensile strength and flexural modulus are very high, which indicates mechanical properties, and it can be predicted that it can be applied to three-dimensional product molding such as cups, straws, and plates. You can.
상기의 본 발명은 바람직한 실험예를 중심으로 살펴보았으며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자는 본 발명의 본질적 기술 범위 내에서 상기 본 발명의 상세한 설명과 다른 형태의 실험예들을 구현할 수 있을 것이다. 여기서 본 발명의 본질적 기술범위는 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다. The above-described present invention has been examined with a focus on preferred experimental examples, and those skilled in the art will be able to implement experimental examples in forms different from the detailed description of the present invention within the essential technical scope of the present invention. You will be able to. Here, the essential technical scope of the present invention is indicated in the patent claims, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (5)
바이오셀룰로오스 괴 제조용 조성물.
Coconut water 70-78% by weight, sugar 1-9% by weight, ammonium sulfate ((NH 4 ) 2 SO 4 ) 0.1-1% by weight, acetic acid (CH 3 COOH) 0.1-1% by weight, magnesium sulfate (MgSO 4 ) 0.001~0.1% by weight, sodium carbonate (Na 2 CO 3 ) 0.001~0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001~0.1% by weight, ammonium dibasic phosphate ((NH 4 ) 2 HPO 4 ) 0.001~0.1% by weight %, characterized by being composed of 20 to 28% by weight of water,
Composition for producing biocellulose lumps.
상기 채집된 코코넛 워터에 물을 2~3 : 1 중량비로 혼합하여 혼합물1을 제조하는 제2단계(S20);
상기 혼합물1에 설탕, 황산암모늄((NH4)2SO4), 황산마그네슘(MgSO4), 탄산나트륨(Na2CO3), 황산칼륨(K2SO4), 제2인산암모늄((NH4)2HPO4)을 넣고 혼합하여 혼합물 2를 제조하는 제3단계(S30);
상기 혼합물2에 아세트산(CH3COOH)를 넣고 혼합하여 코코넛 워터 70~78중량%, 설탕 1~9중량%, 황산암모늄((NH4)2SO4) 0.1~1중량%, 아세트산(CH3COOH) 0.1~1중량%, 황산마그네슘(MgSO4) 0.001~0.1중량%, 탄산나트륨(Na2CO3) 0.001~0.1중량%, 황산칼륨(K2SO4) 0.001~0.1중량%, 제2인산암모늄((NH4)2HPO4) 0.001~0.1중량%, 물 20~28중량%로 구성된 바이오셀룰로오스 괴 제조용 조성물을 제조하는 제4단계(S40);
상기 바이오셀룰로오스 괴 제조용 조성물을 가열하여 살균물을 제조하는 제5단계(S50) 및,
상기 살균물 1L당 아세토박토 자일리늄(Acetobacter xylinum) 배양물 40~50 ml를 넣은 후, 이를 평평한 용기에 담고 이 용기를 천으로 감싼 후, 25~30℃에서 2~3일간 1차 배양한 다음, 천을 제거한 후 25~30℃에서 4~6일간 배양한 후 이를 40~50시간동안 압축탈수하여 바이오셀룰로오즈 괴를 제조하는 제6단계(S60);를 포함하는,
바이오셀룰로오스 괴의 제조방법.
The first step of collecting coconut water (S10);
A second step (S20) of preparing mixture 1 by mixing the collected coconut water with water at a weight ratio of 2 to 3: 1;
In the mixture 1, sugar, ammonium sulfate ((NH 4 ) 2 SO 4 ), magnesium sulfate (MgSO 4 ), sodium carbonate (Na 2 CO 3 ), potassium sulfate (K 2 SO 4 ), and dibasic ammonium phosphate ((NH 4 ) 2 HPO 4 ) is added and mixed to prepare mixture 2 (S30);
Acetic acid (CH 3 COOH) was added to mixture 2 and mixed to obtain 70 to 78% by weight of coconut water, 1 to 9% by weight of sugar, 0.1 to 1% by weight of ammonium sulfate ((NH 4 ) 2 SO 4 ), and acetic acid (CH 3 COOH) 0.1~1% by weight, magnesium sulfate (MgSO 4 ) 0.001~0.1% by weight, sodium carbonate (Na 2 CO 3 ) 0.001~0.1% by weight, potassium sulfate (K 2 SO 4 ) 0.001~0.1% by weight, diphosphate A fourth step (S40) of preparing a composition for producing biocellulose lumps consisting of 0.001 to 0.1% by weight of ammonium ((NH 4 ) 2 HPO 4 ) and 20 to 28% by weight of water;
A fifth step (S50) of producing a sterilizing product by heating the composition for producing biocellulose lumps,
After adding 40 to 50 ml of Acetobacter xylinum culture per 1L of the above sterilizing agent, placing it in a flat container, wrapping the container with a cloth, and primary culturing at 25 to 30°C for 2 to 3 days. , the sixth step (S60) of removing the cloth, culturing it at 25-30°C for 4-6 days, and compressing and dehydrating it for 40-50 hours to produce biocellulose lumps (S60);
Method for producing biocellulose lumps.
바이오셀룰로오스 괴.Manufactured by the manufacturing method of paragraph 3,
Biocellulose lump.
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