CN111990171B - Preparation method and application of mushroom mycelium bio-based composite material - Google Patents
Preparation method and application of mushroom mycelium bio-based composite material Download PDFInfo
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- CN111990171B CN111990171B CN202010755358.0A CN202010755358A CN111990171B CN 111990171 B CN111990171 B CN 111990171B CN 202010755358 A CN202010755358 A CN 202010755358A CN 111990171 B CN111990171 B CN 111990171B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention relates to a preparation method and application of a mushroom mycelium bio-based composite material, and belongs to the technical field of bio-based materials. The preparation method comprises the following steps: preparing a biological culture medium; inoculating the mushroom strain to a biological culture medium, and performing primary hypha culture; pulverizing the product obtained by the first hypha culture to obtain invisible white hypha, adding water and wheat flour, mixing, placing in a mold, and performing the second hypha culture to obtain a semi-finished product; baking, shaping and sterilizing the semi-finished product, taking out and airing to obtain the mushroom mycelium bio-based composite material; the preparation method of the mushroom mycelium bio-based composite material has the advantages of good forming effect and excellent mechanical property.
Description
Technical Field
The invention belongs to the technical field of bio-based materials, and relates to a preparation method and application of a mushroom mycelium bio-based composite material.
Background
At present, most of transport packaging and heat insulation materials are petroleum-based polymers such as XPS or EPS and the like, are difficult to decompose in a short time in natural environment, are main materials causing white pollution, destroy water quality and soil structure, and aggravate greenhouse effect after incineration treatment; meanwhile, petroleum resources for preparing petroleum-based polymers are non-renewable resources and are increasingly exhausted under the condition of over-development. Agricultural wastes such as sawdust, corncobs and straws are selected to be burned in the field by farmers without other ways, so that air is polluted.
With the increasing concern of human beings on environment and energy, the biobased materials gradually come into the visual field of people with good degradability. The bio-based composite material is characterized in that the composite reconstruction of lignocellulose agricultural and forestry wastes such as straws except for grains and other materials is realized through a special processing technology, and a new material meeting different level requirements of human beings is processed.
As one of the bio-based composite materials, as early as 2007, Eben Bayer and Gavin McIntyre in the United states have reported related technologies for preparing mycelium-based plastics (commonly called mushroom plastics) by using fungal cultivation, and an ecovision Design LLC company is established in a short time and is put into industrial preparation and application. The technology uses the agricultural waste with low cost as the raw material, and the obtained mycelium-based plastic has good biodegradability and comprehensive performance, thereby rapidly arousing strong interest in the academic world, the industrial industry and the investment world. The mycelium composite material is expected to become a substitute of a plastic packaging material, the use of the mycelium composite material can greatly reduce the exploitation and processing of petroleum, reduce the utilization rate of non-renewable resources in the environment, increase the environmental protection, and realize the synchronous sustainable development of the environment and resources. Statistically, the global demand for biodegradable polymers in 2018 is 36 million tons, and the market amount exceeds 11 hundred million dollars; by 2023, the demand of biodegradable polymers worldwide will be expected to increase by over 50%, reaching 55 million tons, with a market amount expected to reach $ 17 million, with enormous market space potential.
In view of the fact that the technology has no application prospect and wide market, and the technology of mycelium-based plastics of ecovision corporation is protected by trade secrets and patent rights and cannot be applied in a large scale, China needs to develop the technology autonomously to cope with the competition of the future market.
Disclosure of Invention
The invention aims to provide a preparation method of a mushroom mycelium bio-based composite material with good forming effect and excellent mechanical property aiming at the problems in the prior art.
The purpose of the invention can be realized by the following technical scheme:
a preparation method of a mushroom mycelium bio-based composite material comprises the following steps:
s1, preparing a biological culture medium;
s2, inoculating the mushroom strains to a biological culture medium, and carrying out primary hypha culture;
s3, crushing the product obtained by the primary hypha culture to obtain invisible white hypha, adding water and wheat flour, uniformly mixing, placing in a mold, and performing secondary hypha culture to obtain a semi-finished product;
and S4, baking, shaping and sterilizing the semi-finished product, taking out and airing to obtain the mushroom mycelium bio-based composite material.
The bio-based composite material is prepared by mixing mushroom strains, sawdust, corncobs, cottonseed hulls, straws, bagasse, wheat bran, gypsum, lime, water, wheat flour and the like according to a certain proportion and performing two times of mycelium culture, and the mushroom mycelia and agricultural and forestry waste are combined, namely the mushroom mycelia are added into the agricultural and forestry waste as an adhesive to form the mushroom mycelium bio-based composite material; the preparation method of the invention is easy to form, the mushroom hypha can grow along the mould, and the biological culture medium materials are combined together. The composite material formed by the method is hard in material, has certain toughness and low density, and the quality of the product after baking and forming can reach one third of that before baking and forming, so that the composite material can be used as a buffer liner package of some products; the composite material formed by the invention has good heat insulation effect and certain water absorption capacity; the degradation period is short, and the water source and soil can be purified; meanwhile, the composite material takes agricultural and forestry wastes as main raw materials, so that the utilization of the agricultural and forestry wastes is realized, and the consumption of non-renewable resource resources is reduced. The dried mushroom mycelium has the characteristics of hardness, lightness and the like, and is firmer compared with other strains.
The first hypha culture can be carried out in a high-temperature sterilization bag, hyphae fully grow, when the hyphae grow to a certain degree, a product of the first hypha culture is crushed, the hyphae can be used as a binder so as to be beneficial to forming of crushed objects in a mold, the hyphae can also be used as a strain for the second hypha culture, and the second hypha culture is carried out in the mold until baking and shaping are carried out.
In the invention, a certain amount of water and wheat flour are added in the secondary hypha culture, wherein the water is used for keeping the culture base material wet all the time, and the wheat flour provides nutrients for hypha, so that the hypha can grow rapidly in the secondary hypha culture.
The composite material finished product prepared by the invention is a perfect substitute of foamed plastic, and can provide a green and environment-friendly material for packaging and transportation, protective clothing industry, building industry and other industries.
Preferably, the method for preparing the biological medium in step S1 includes mixing raw materials of the medium including wood chips, agricultural wastes, wheat bran, gypsum, lime, adjusting the water content to 55-60%, bagging and sterilizing.
According to the invention, the water content of the biological culture medium is adjusted to 55-60% in advance, so that the growth and propagation of the mushroom hyphae can be promoted, and the forming efficiency of the composite material is accelerated.
Preferably, the mass percentages of the wood chips, the agricultural wastes, the wheat bran, the gypsum and the lime are 60-85%, 3-20%, 10-20%, 0.5-1.5% and 0.5-1.5%.
Preferably, the agricultural waste comprises one or more of corncobs, cottonseed hulls, straw or bagasse.
The invention obtains the composite material with good mechanical property by optimally and scientifically proportioning the raw materials of the biological culture medium.
Preferably, the particle size range of the sawdust is 3-6mm, the particle size range of the agricultural waste is 1-3mm, and the particle size range of the wheat bran is 40-60 μm.
The mushroom mycelium bio-based composite material is formed by injection molding and high-temperature inactivation, the particle size of the raw materials has certain influence on the performance of the final composite material, and the large particle size can cause uneven contact and incomplete bonding between the raw materials, further cause rough surface of the material and poor product performance; the smaller particle size results in a decrease in the mechanical strength and an increase in weight of the shiitake mushroom mycelium bio-based composite.
Preferably, the raw materials of the culture medium further comprise one or two of hemp grass and gauze.
Further preferably, the mass percentage of the linen or/and the gauze in the raw materials of the culture medium is 2-5%.
According to the invention, one or two of the linen and the gauze are added into the raw materials of the culture medium, the linen and the gauze can improve the ductility of the base material in the composite material, so that the base material is combined more tightly, and meanwhile, the gaps among the base material particles are enlarged, therefore, hyphae can grow better, the porosity of the composite material can be further improved, the composite material is more suitable for being used as a moisture-proof and moisture-absorbing material, the weight of the composite material is greatly reduced, and the composite material is more suitable for being used as a product with light weight requirements, such as a heat-insulating foam board.
Preferably, the sterilization is autoclaving at 110-.
The invention can effectively avoid the growth structure of the mycelium from being damaged by the mixed bacteria in the raw materials through high-temperature and high-pressure disinfection and sterilization.
Preferably, the inoculation in step S2 is performed on a sterile operating table.
Preferably, the first mycelium culture in step S2 is carried out at a constant temperature of 20-30 deg.C under no light and low oxygen conditions for 40-70 days, and the second mycelium culture in step S3 is carried out at a constant temperature of 20-30 deg.C under no light and low oxygen conditions for 7-10 days, or at a constant temperature of 20-30 deg.C under no light and low oxygen conditions for 7-10 days, and further carried out at 18-25 deg.C under ventilation conditions for 15-18 days to change the color of the mycelium.
Preferably, the second mycelium culture comprises culturing at 20-30 deg.C under constant temperature, no light and low oxygen condition for 7-10 days, and further culturing at 18-25 deg.C under ventilation condition for 15-18 days to change color of mycelium.
The hyphae can densely wrap the biological culture medium when the first hyphae is cultured for 40-70 days, the hyphae can densely wrap the biological culture medium when the second hyphae is cultured for 7-10 days, and the obtained hyphae composite material can obtain better mechanical property and moisture-proof heat-preservation sound-insulation property after being inactivated. When the hyphae are cultured for the second time to 24-28 days, the hyphae can reach a specific physiological maturity, the hyphae is lodged and secretes pigments, the color is gradually changed from white to light brown to brown and tan, and finally a layer of tan fungus membrane integrating multiple functions of heat preservation, moisture preservation, light protection, foreign bacteria resistance and the like is formed, so that the color changing process is completed, the water retention performance of the hypha composite material inactivated after color changing is better and firmer, and various mechanical properties, particularly the bending resistance, of the hypha composite material are obviously improved compared with the hypha composite material prepared without color changing and inactivation.
Preferably, the low oxygen is 20-50% oxygen content.
The invention obtains proper oxygen content by putting the biological culture medium into a sealing bag with small holes or a sealing bag with a high-density filter screen.
According to the invention, the temperature and oxygen content of the mushroom mycelium culture have certain influence on the formation time of the mushroom mycelium bio-based composite material, and the temperature is too high or too low, and the oxygen content is too high or too low, so that the growth speed of the mycelium is influenced, and therefore, the temperature and the oxygen content of the culture medium are controlled to improve the growth speed of the mycelium.
Preferably, the water and wheat flour are added to the first mycelium culture product in the weight percentages of 0.5-1.5% and 0.5-1.5%, respectively, in step S3.
Further preferably, the water and wheat flour are added in a weight ratio of 1: 1.
Preferably, the material of the mold in step S3 is plastic or wood fiber.
The material of the mold for shaping has certain influence on the growth of the mushroom mycelium bio-based composite material, and the common silica gel mold can reduce the activity of the mushroom mycelium, so the plastic or wood fiber mold is adopted for shaping.
Preferably, the baking temperature of step S4 is 90-95 deg.C, and the baking time is 30-40 minutes.
The invention also aims to provide an anti-impact, heat-insulating or moisture-proof moisture-absorption bio-based composite material which is prepared by adopting the preparation method of the mushroom mycelium bio-based composite material.
Compared with the prior art, the invention has the following beneficial effects: .
(1) The mushroom mycelium bio-based composite material is prepared by compounding and fermenting mushroom mycelium serving as a biological adhesive and a culture medium taking agricultural and forestry waste as a main raw material, so that the agricultural and forestry waste is recycled;
(2) when the prepared mushroom mycelium bio-based composite material is discarded, the composite material can be automatically degraded and purify water sources and soil, the degradation period is short, and the environment-friendly effect is achieved;
(3) the mushroom mycelium bio-based composite material prepared by the invention has excellent mechanical property and can be used as a substitute of foamed plastic;
(4) the mushroom mycelium bio-based composite material prepared by the invention has the advantages of low cost, light weight, good heat insulation performance and the like, and can realize additional effects of moisture prevention, moisture absorption, heat preservation and the like. Will have wide application in the industrial field;
(5) the invention adopts a method of mycelium culture twice, so that the quality of the prepared mushroom mycelium bio-based composite material is more homogenized, the mechanical property is more excellent, and the shaping of the mushroom mycelium bio-based composite material is conveniently realized;
(6) the invention finds a good way for the optimization and adjustment of the Chinese agricultural industrial structure, and has great significance for promoting the utilization of Chinese waste resources and the technical innovation in the field of environment-friendly materials and realizing the spanning development of high-technology industrial groups such as biology, environment, materials and the like.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and that those skilled in the art can also obtain other related drawings based on the drawings without inventive efforts.
FIG. 1 is a photograph of a final composite material prepared in example 1 of the present invention;
FIG. 2 is a photograph of a final composite material obtained in example 2 of the present invention;
FIG. 3 is a photograph of a final composite material obtained in example 3 of the present invention;
FIG. 4 is a photograph of a final composite material obtained in example 3 of the present invention.
Detailed Description
The following are specific examples of the present invention and further describe the technical solutions of the present invention, but the present invention is not limited to these examples.
Example 1
The preparation method of the mushroom mycelium bio-based composite material in the embodiment comprises the following steps:
(1) crushing sawdust to a particle size range of 3-6mm and an average particle size of 4.6mm in advance, crushing corncobs to a particle size range of 1-3mm and an average particle size of 2.2mm, crushing wheat bran to a particle size range of 40-60 mu m and an average particle size of 51 mu m, mixing 78% of sawdust, 5% of corncobs, 15% of wheat bran, 1% of gypsum and 1% of lime according to mass percentage, adjusting the water content to 56%, then bagging, and carrying out high-pressure steam sterilization for 25 minutes under the conditions of 121 ℃ and 0.15kpa to prepare a biological culture medium;
(2) inoculating Lentinus Edodes strain to biological culture medium on aseptic operation table, and performing primary mycelium culture at 25 deg.C under constant temperature, no light and oxygen content of 20% for 45 days;
(3) pulverizing the product obtained by the first hypha culture to invisible white hypha, adding water and wheat flour, adding 1% and 1% of the product obtained by the first hypha culture, mixing, placing in a plastic mold, and performing the second hypha culture at constant temperature of 25 deg.C under the conditions of no light and oxygen content of 30% for 8 days to obtain semi-finished product;
(4) and (3) baking the semi-finished product at 93 ℃ for 40 minutes for shaping and sterilizing, taking out and drying to obtain the mushroom mycelium bio-based composite material, wherein the composite material prepared in the embodiment is shown in figure 1.
Example 2
The preparation method of the mushroom mycelium bio-based composite material in the embodiment is as follows:
(1) crushing sawdust to a particle size range of 3-6mm and an average particle size of 4.6mm in advance, crushing cottonseed hulls to a particle size range of 1-3mm and an average particle size of 2.2mm, crushing wheat bran to a particle size range of 40-60 mu m and an average particle size of 51 mu m, mixing 69% of sawdust, 12% of cottonseed hulls, 14% of wheat bran, 1% of gypsum, 1% of lime and 3% of hemp in percentage by mass, adjusting the water content to 55%, bagging, and sterilizing with high-pressure steam at the conditions of 121 ℃ and 0.15kpa for 25 minutes to prepare a biological culture medium;
(2) inoculating Lentinus Edodes strain to biological culture medium on aseptic operation table, and performing primary mycelium culture at 23 deg.C under constant temperature, no light, and oxygen content of 20% for 49 days;
(3) pulverizing the product obtained by the first hypha culture to obtain invisible white hypha, adding water and wheat flour, adding 1.3% and 1.2% of the product obtained by the first hypha culture, mixing, placing in a plastic mold, and performing the second hypha culture at 23 deg.C under conditions of no light and 20% oxygen content for 9 days to obtain semi-finished product;
(4) and (3) baking the semi-finished product at 95 ℃ for 30 minutes for shaping and sterilizing, taking out and drying to obtain the mushroom mycelium bio-based composite material, wherein the composite material prepared in the embodiment is shown in figure 2.
Example 3
The preparation method of the mushroom mycelium bio-based composite material in the embodiment comprises the following steps:
(1) crushing sawdust into particles with the size range of 3-6mm and the average particle size of 4.6mm in advance, crushing cottonseed hulls into particles with the size range of 1-3mm and the average particle size of 2.2mm, crushing wheat bran into particles with the size range of 40-60 mu m and the average particle size of 51 mu m, preparing 72% of sawdust, 5% of straw, 5% of bagasse, 14% of wheat bran, 2% of gauze, 1% of gypsum and 1% of lime according to mass percentage, mixing the sawdust, the straw, the bagasse, the wheat bran, the gypsum and the lime, adjusting the water content to 57%, bagging the mixture for high-pressure steam sterilization for 25 minutes under the conditions of 121 ℃ and 0.15kpa to prepare a biological culture medium;
(2) inoculating Lentinus Edodes strain to biological culture medium on aseptic operation table, and performing primary mycelium culture at 27 deg.C under constant temperature, no light, and oxygen content of 20% for 46 days;
(3) pulverizing the product obtained by the first hypha culture to invisible white hypha, adding water and wheat flour, stirring, adding 1.2% and 0.9% of the product obtained by the first hypha culture, adding gauze, mixing, placing in a plastic mold, and performing the second hypha culture, namely culturing for 7 days at a constant temperature of 27 ℃, in the absence of light and under an oxygen content of 40%, thus obtaining a semi-finished product;
(4) and (3) baking the semi-finished product at 93 ℃ for 40 minutes for shaping and sterilizing, taking out and drying to obtain the mushroom mycelium bio-based composite material, wherein the composite material prepared in the embodiment is shown in figure 3.
Example 4
In the second hypha culture, the mycelia were cultured at constant temperature of 25 deg.C under no light and low oxygen conditions for 8 days, and then cultured at temperature of 22 deg.C under ventilation for 16 days to turn the color of the mycelia, otherwise the composite material obtained in this example was the same as that of example 1, and is shown in FIG. 4.
Comparative example 1
The inoculated culture medium is placed in a mould for one-time fermentation setting, and the rest is the same as the example 1.
Comparative example 2
Commercially available EPS material sheets.
In the examples and comparative examples of the present invention, the proper oxygen content was obtained by placing the biological medium in a sealed bag with a small hole or a sealed bag provided with a high-density sieve.
The performance of the Lentinus edodes mycelium composite materials prepared in examples 1-5 and comparative examples 1-2 of the present invention was compared, and the comparison results are shown in Table 1. The performance test of the composite materials in each of the examples and comparative examples in Table 1 was carried out according to the method recommended in GB5486 test method for inorganic hard thermal insulation articles, specifically as follows:
(I) Density test method step
(1) Measuring the mass m of the test piece on an electronic scale, reserving four valid figures,
(2) measuring the geometric size of the sample according to the method of GB5486, and calculating the volume V of the sample;
the density p of the test piece is calculated according to the formula p ═ m/v in kg/m3。
(II) test method steps of compressive strength
(1) Making a test piece into a standard piece required by a tension and pressure testing machine, starting the testing machine, adjusting the compression surface and the pressure bearing plate when the test piece is close to the pressure plate to ensure that the contact surface is in uniform contact, and recording the compression area of the test piece as S;
(2) and slowly loading the test piece until the test piece is damaged, and recording the compression deformation value. If no failure occurred while the sample was at 5% compressive deformation, the load at 5% compressive deformation of the sample is recorded as the failure load. Recording the failure load as P;
the compressive strength sigma of the test piece is calculated according to the formula sigma P/S, and the unit kpa
(III) method for testing bending strength
(1) Measuring the width b and the thickness h of the test piece;
(2) placing a test piece in a testing machine, and recording the central distance l between support supporting rollers below the test piece;
(3) uniformly loading, pressurizing until the test piece is damaged, and recording the maximum damage load P;
the compressive strength tau of the test piece is 3Pl/2bh according to the formula tau2Calculate, unit kpa.
(IV) Water absorption test method steps
(1) Weighing the mass m1 of the test piece;
(2) placing the test piece into a water tank, adding water to a position 25mm higher than the test piece, and soaking for 2 hours;
(3) taking out the test piece, placing the test piece on a dry towel for draining for 10min
(4) Weighing the mass m2 of the test piece again after the moisture on the surface of the test piece is absorbed;
the mass water absorption w of the test piece was calculated in the unit% according to the formula w ═ m2-m1)/m 1.
(V) testing method steps of dimensional stability
(1) Measuring the distance L1 between the length and the width of the test piece;
(2) horizontally placing the measured test piece in a high-temperature furnace, and carrying out constant-temperature treatment at 90 ℃ for 24 hours;
(3) cooling the heat-treated test piece to room temperature, and checking the crack and warpage conditions of the test piece;
(4) measuring the distance L2 between the length and the width of the test piece;
the mass dimensional stability H of the test pieces was calculated in unit% according to the formula H ═ L1-L2)/L1 × 100.
(VI) method for testing heat insulation performance of material
The test piece is tested by using a DRE-III type thermal conductivity tester, and the data obtained by the test is substituted into a thermal resistance formula U which is 1/Rt (U is a heat transfer coefficient (W/m)2K) and Rt is a thermal resistance value (K m)2/W))。
Table 1: properties of the composite materials obtained in examples 1 to 5 and comparative examples 1 to 2
As can be seen from the table 1, the compressive strength of the composite material prepared in the embodiment of the invention is not much different from that of an EPS material plate, the water absorption rate is far greater than that of the EPS material, and the composite material can be used as an impact-resistant, heat-insulating, moisture-absorbing and moisture-proof material; the hemp grass is added into the material prepared in the embodiment 2 or 3, so that the material has better ductility and higher porosity, and is more suitable for being used as a sound-insulation heat-insulation material and a moisture-proof and moisture-absorption material, and the mechanical properties of the composite material prepared by color conversion of hypha in the embodiment 4 are obviously improved compared with the hypha composite material prepared by non-color-conversion inactivation.
The technical range of the embodiment of the invention is not exhaustive, and new technical solutions formed by equivalent replacement of single or multiple technical features in the technical solutions of the embodiment are also within the scope of the invention; in all the embodiments of the present invention, which are listed or not listed, each parameter in the same embodiment represents only one example (i.e., a feasible embodiment) of the technical solution, and there is no strict matching and restriction relationship between the parameters, wherein the parameters can be replaced with each other without departing from the axiom and the requirements of the present invention, unless otherwise specified.
The technical means disclosed by the scheme of the invention are not limited to the technical means disclosed by the technical means, and the technical means also comprises the technical scheme formed by any combination of the technical features. While the foregoing is directed to embodiments of the present invention, it will be appreciated by those skilled in the art that various changes and modifications may be made without departing from the principles of the invention, and it is intended that all such changes and modifications be considered as within the scope of the invention.
Claims (6)
1. A preparation method of a mushroom mycelium bio-based composite material is characterized by comprising the following steps:
s1, preparing a biological culture medium;
s2, inoculating the mushroom strains to a biological culture medium, and carrying out primary hypha culture;
s3, crushing the product obtained by the primary hypha culture to obtain invisible white hypha, adding water and wheat flour, uniformly mixing, placing in a mold, and performing secondary hypha culture to obtain a semi-finished product;
s4, baking, shaping and sterilizing the semi-finished product, taking out and airing to obtain the mushroom mycelium bio-based composite material;
the first hypha culture in step S2 is carried out for 40-70 days under the conditions of constant temperature of 20-30 ℃, no light and low oxygen, the second hypha culture in step S3 is carried out for 7-10 days under the conditions of constant temperature of 20-30 ℃, no light and low oxygen or is carried out for 7-10 days under the conditions of constant temperature of 20-30 ℃, no light and low oxygen, and then the hypha is continuously cultured for 15-18 days under the conditions of 18-25 ℃ and ventilation to change the color of the hypha;
the raw materials of the culture medium comprise sawdust, agricultural wastes, wheat bran, gypsum and lime; the weight percentage of the wood chips, the agricultural wastes, the wheat bran, the gypsum and the lime is 60-85%, 3-20% of the agricultural wastes, 10-20% of the wheat bran, 0.5-1.5% of the gypsum and 0.5-1.5% of the lime; the particle size range of the sawdust is 3-6mm, the particle size range of the agricultural waste is 1-3mm, and the particle size range of the wheat bran is 40-60 mu m;
the raw materials of the culture medium also comprise one or two of rough grass and gauze.
2. The method of preparing a Lentinus edodes mycelium bio-based composite material according to claim 1, wherein the step S1 comprises mixing the raw materials of the culture medium, adjusting the water content to 55-60%, bagging and sterilizing.
3. The method of preparing a champignon mycelium bio-based composite material according to claim 1, wherein the agricultural waste comprises one or more of corncobs, cottonseed hulls, straw, or bagasse.
4. The method of claim 1, wherein the hypoxia is 20-50% oxygen content.
5. The method for preparing mushroom mycelium bio-based composite material according to claim 1, wherein the baking temperature of the step S4 is 90-95 ℃ for 30-40 minutes.
6. An impact, thermal or moisture resistant hygroscopic bio-based composite material characterised in that it is a composite material made by a method as claimed in any one of claims 1 to 5.
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| US11920126B2 (en) | 2018-03-28 | 2024-03-05 | Ecovative Design Llc | Bio-manufacturing process |
| US11864676B2 (en) * | 2021-03-03 | 2024-01-09 | Michaela Ceci | Biodegradable hanger for clothing, and method for making the same |
| CA3217304A1 (en) * | 2021-05-20 | 2022-11-24 | Elizabeth MONAGHAN | Headgear for supporting a living plant and method for using the same |
| CN114175968A (en) * | 2021-11-11 | 2022-03-15 | 鲁东大学 | Composite material based on wood rotting fungus mycelia and preparation method and application thereof |
| CN115104479A (en) * | 2022-07-29 | 2022-09-27 | 衡水职业技术学院 | Edible fungus planting co-production method |
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| CN105660176A (en) * | 2015-08-19 | 2016-06-15 | 东营艾格林生物技术有限公司 | Production process for biologic mycelium material |
| CN108370817B (en) * | 2016-10-12 | 2020-08-04 | 深圳市泽青源科技开发服务有限公司 | Method for recycling mushroom bran |
| KR101929457B1 (en) * | 2017-03-29 | 2018-12-14 | (주)해나눔 | Culture method for mass producing Tricholoma matsutake mycelium |
| CN108934760A (en) * | 2018-07-11 | 2018-12-07 | 鲁东大学 | A method of degradable package material is prepared using Schizophyllum commune for Mycelium |
| CN109588208B (en) * | 2018-12-22 | 2021-09-03 | 甘肃省科学院生物研究所 | Biological porous material and preparation method thereof |
| CN110713370A (en) * | 2019-11-18 | 2020-01-21 | 常熟理工学院 | Preparation method of composite environment-friendly building material |
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