KR102644597B1 - Antioxidative composition having improved stability - Google Patents

Abstract

The present invention relates to an antioxidant composition, and more specifically, to mulberry extract; It relates to an antioxidant composition containing at least one selected from the group consisting of epigallocatechin gallate, lutein, and astaxanthin.
The antioxidant composition according to the present invention not only has an excellent antioxidant effect, but also improves the stability of antioxidant substances, thereby increasing the sustainability of the effect, thereby obtaining a better antioxidant effect, and is not harmful to the human body, enabling the manufacture of various products such as medicines and foods. .

Description

Antioxidant composition with improved stability {ANTIOXIDATIVE COMPOSITION HAVING IMPROVED STABILITY}

The present invention relates to an antioxidant composition with improved stability.

Oxygen is an essential element for various metabolic reactions to sustain life and is also necessary for detoxification of toxic substances in the human body, but oxygen is not only beneficial to the human body. Specifically, superoxide radicals (O ₂ · ^- ) and hydroxyl radicals (·OH) are generated by various physical, chemical and environmental factors such as the body's enzyme system, reduction metabolism, chemicals, pollutants and photochemical reactions. ), hydrogen peroxide (H ₂ O ₂ ), and singlet oxygen ( ¹ O ₂ ), which are converted into reactive oxygen species (ROS), which are highly reactive free radicals. It has the double effect of causing fatal oxygen toxicity to living organisms.

Reactive oxygen species are produced by antioxidant enzymes such as superoxide dismutase (SOD), catalase, peroxidase, and glutathione, which are the body's defense mechanisms, and vitamin C (ascorbic acid). It can be minimized by the action of antioxidant substances such as vitamin E (tocopherol). However, as exposure to various harmful environments and stresses has increased in modern times, if reactive oxygen species are excessively produced in the body or biological defenses are impaired, reactive oxygen species destroy cellular components such as lipids, proteins, sugars, and DNA. It is known to cause cell aging or various diseases, including cancer. In addition, various peroxides in the body, including lipid peroxides, which are generated as a result of lipid peroxidation by these reactive oxygen species, cause oxidative destruction of cells, causing various functional disorders and causing various diseases. Accordingly, much development and research is being conducted on antioxidants that inhibit oxidation by reactive oxygen species.

To remove these reactive oxygen species, synthesis of butylhydroxyanisole (t-Butyl-4-hydroxyanisole: BHA), dibutylhydroxytoluene (3,5-(t-Butyl)-4-hydroxytoluene: BHT), etc. Antioxidants have been widely used due to their excellent antioxidant effect and economic efficiency, but due to problems such as liver enlargement, toxicity of substances absorbed in the body, and the possibility of carcinogenesis, the foods allowed and the amount of use are strictly limited.

Meanwhile, research on natural antioxidants that are harmless to the human body and have excellent antioxidant power has been conducted for a long time. Natural antioxidants such as catechin, carotenoid, vitamin C, and vitamin E have a strong antioxidant effect by suppressing reactive oxygen species and are highly safe for the human body, but are expensive and are structurally unstable, so they can be used in the air, especially oxygen. It has the disadvantage of being easily decomposed by oxidation as it reacts sensitively to external environments such as heat and light. For this reason, various techniques have been proposed to improve the stability of antioxidants.

For example, Republic of Korea Patent No. 10-0929703 discloses that the stability of antioxidants can be increased by coating a core containing an antioxidant compound with a polymer of an alpha-lipoic acid-based compound.

In addition, Republic of Korea Patent No. 10-1567354 discloses (A) UV scattering agent; (B) An emulsion composition containing an active oxygen scavenger and an antioxidant, wherein the stability of the composition can be improved by separately including (A) and (B) in different phases of the oil phase and the water phase of the emulsion composition. It is starting.

These patents have some effectiveness in preventing oxidation of antioxidants, but the effect is not sufficient. Therefore, there is a greater need for the development of antioxidant compositions with excellent stability.

Republic of Korea Patent No. 10-0929703 (2009.11.25), coated antioxidant particles, composition containing the same, and method for producing the same Republic of Korea Patent No. 10-1567354 (2015.11.03), Emulsion composition

Accordingly, the present inventors conducted research from various angles to solve the above-mentioned problems, and as a result, when mulberry compounds containing cyanidin-3-glucoside, which is effective in stabilizing antioxidant substances, are included in a certain amount or more together with antioxidant substances, the antioxidant substances It was confirmed that the stability of was improved and showed excellent antioxidant effect.

Accordingly, the purpose of the present invention is to provide an antioxidant composition with improved stability.

To achieve the above object, the present invention provides an antioxidant composition comprising mulberry extract and at least one selected from the group consisting of epigallocatechin gallate, lutein, and astaxanthin.

The mulberry extract may contain cyanidin-3-glucoside.

The mulberry extract may be one or more extracts selected from the group consisting of water and organic solvents.

The mulberry extract may be included in an amount of 45 to 60% by weight based on 100% by weight of the total antioxidant composition.

The epigallocatechin gallate may be included in an amount of 2 to 5% by weight based on 100% by weight of the total antioxidant composition.

The lutein may be included in an amount of 30 to 40% by weight based on 100% by weight of the total antioxidant composition.

The astaxanthin may be included in an amount of 6 to 10% by weight based on 100% by weight of the total antioxidant composition.

Additionally, the present invention provides a pharmaceutical composition containing the antioxidant composition.

In addition, the present invention provides a food composition containing the antioxidant composition.

The antioxidant composition according to the present invention contains mulberry extract containing a large amount of cyanidin-3-glucoside, thereby improving the stability of the antioxidant substance and increasing the sustainability of the antioxidant effect of the antioxidant composition. In addition, it is easy to store and process, making it possible to manufacture it into various products such as medicine and food, making industrialization possible.

Figure 1 is a graph showing the stability evaluation results of epigallocatechin gallate according to Experimental Example 2 of the present invention.
Figure 2 is a graph showing the results of evaluating the stability of lutein according to Experimental Example 2 of the present invention.
Figure 3 is a graph showing the stability evaluation results of astaxanthin according to Experimental Example 2 of the present invention.

Hereinafter, the present invention will be described in more detail.

Terms or words used in this specification and claims should not be construed as limited to their ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his or her invention in the best way. It must be interpreted with meaning and concept consistent with the technical idea of the present invention based on the principle that it is.

The terms used in the present invention are only used to describe specific embodiments and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the present invention, terms such as 'include' or 'have' are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features or It should be understood that this does not exclude in advance the possibility of the presence or addition of numbers, steps, operations, components, parts, or combinations thereof.

The term “antioxidation” as used herein refers to inhibiting oxidation of cells caused by reactive oxygen species, and includes reducing cellular damage caused by removing reactive oxygen species.

The present invention provides an antioxidant composition with excellent stability.

Recently, various harmful stimuli from the outside, such as radiation, ozone, heavy metals, fine dust, ultraviolet rays, and cigarettes, increase oxygen radicals in the body, and as we age, the ability of the body's antioxidant system gradually decreases. As a result, reactive oxygen species that exceed self-defense capabilities are generated in the body, which not only accelerates aging but also causes many diseases such as cancer, diabetes, arthritis, cerebrovascular disease, cardiovascular disease, dementia, etc.

For this reason, there is a need to supply the body with substances that can remove reactive oxygen species, that is, antioxidants, so that the body can defend itself by consuming antioxidant substances. In response to the demand for supply of antioxidants to the body, the development and research of antioxidants are actively progressing, and many antioxidants, such as synthetic antioxidants and natural antioxidants, are known. In the case of synthetic antioxidants, as their mutagenicity and toxicity have been pointed out, preference for natural antioxidants that are safer for the human body and have excellent antioxidant power is increasing.

However, in the case of natural antioxidants, they are structurally unstable and are easily oxidized and lose their original properties under normal conditions, that is, at room temperature or higher, causing many difficulties in industrialization and commercialization.

In the prior art, various methods have been proposed, such as chemical methods such as adding antioxidants and stabilization in emulsified compositions, or physical methods such as forming a coating layer and sealing with oxygen-impermeable packaging materials, but the stability of natural antioxidants has not been effectively improved. I couldn't do it.

Accordingly, the present invention proposes an antioxidant composition containing ingredients that improve the stability of antioxidants in order to ensure a stable and continuous antioxidant effect of the antioxidant composition.

Specifically, the antioxidant composition according to the present invention includes mulberry extract and one or more antioxidant substances selected from the group consisting of epigallocatechin gallate (EGCG), lutein, and astaxanthin. .

In the present invention, mulberry is the fruit of Morus alba L., a deciduous tree belonging to the Moraceae family. It is harvested from May to June when the color of the fruit is black or magenta and eaten. It is used as herbal medicine after being dried or dried. The mulberries may be varieties such as Cheongil mulberry, Iksu mulberry, Suwon mulberry, and Gwasang No. 2, and cultivated, collected, or commercially available types may be used. The mulberries have higher cyanidin-3-glucoside content than the same berry fruits such as blueberry, cranberry, blackberry, raspberry, and black raspberry. It has 3-glucoside (C3G) content and has the effect of improving the stability of antioxidant substances.

The mulberry extract of the present invention contains cyanidin-3-glucoside, and the cyanidin-3-glucoside has excellent antioxidant power and stabilizes antioxidant substances by being oxidized instead of antioxidant substances. In particular, the mulberry extract of the present invention increases the stability of antioxidant substances by containing cyanidin-3-glucoside in a certain amount or more, exhibits a stable and continuous antioxidant effect, and can be applied to products for various purposes. The cyanidin-3-glucoside content in the mulberry extract may be 0.4% (w/w) or more, preferably 0.4 to 0.8% (w/w), and within the content range, oxidation of antioxidant substances is suppressed. It can increase the stability of antioxidant substances.

The mulberry extract used in the present invention is not limited to the mulberry itself, but is a leachate obtained by leaching or transferring from mulberries, a concentrate obtained by concentrating part or all of the leachate again, or a precipitate prepared by drying the concentrate again. It includes premise, regular, liquid extract, etc.

For example, the mulberry extract can be obtained according to a common natural product extraction method in which a predetermined extraction solvent is added to the raw mulberry, immersed for a certain period of time, stirred at appropriate time intervals, and then the resulting solution is filtered and recovered. At this time, the mulberries may be fresh or dried by any method such as natural drying or forced drying and then ground.

The extraction solvent may be one or more selected from the group consisting of water and organic solvents. At this time, the organic solvent is one or more selected from the group consisting of C1 to C6 alcohols such as methanol and ethanol, acetone, ether, diethyl ether, ethyl acetate, ethyl methyl ketone, chloroform, glycerin, propylene glycol, and butylene glycol. It can be included. Preferably, the extraction solvent may be one or more selected from the group consisting of water, methanol, ethanol, propanol, and acetone, and more preferably ethanol. At this time, the extraction solvent can be used in an amount of about 1 to 50 times the total volume of the raw materials.

In the antioxidant composition of the present invention, in order for the mulberry extract to contain a high content of cyanidin-3-glucoside, which is an effective ingredient in stabilizing antioxidant substances as described above, the extraction solvent is extracted with ethanol at a concentration of 60 to 95%. It is desirable. More preferably, the concentration of ethanol is 60 to 80%, more preferably 65 to 75%, and when ethanol in the corresponding concentration range is used, cyanidin-3-glucoside can be extracted at high concentration. In the present invention, by extracting with 60 to 95% ethanol, destruction of the active ingredients of the mulberry extract contained in the composition according to the present invention can be suppressed and a high yield of the active ingredients can be obtained. As a result of comparing the content of cyanidin-3-glucoside in the mulberry extract extracted with 70% ethanol in Experimental Example 1 of the present invention and the mulberry extract using ethanol outside the above range as an extraction solvent, including distilled water, the mulberry extract using 70% ethanol was found to be In the case of the extract, it was confirmed that the content of the corresponding ingredient was higher.

The extraction method may use conventional extraction methods such as hot water extraction, immersion extraction, reflux extraction, reflux cooling extraction, and ultrasonic extraction.

After obtaining the extract, a liquid product can be obtained by cold immersion, heating, and filtration at room temperature using conventional methods known in the art, or an additional process of evaporating the solvent, spray drying, or freeze drying can be performed. .

The mulberry extract may be included in an amount of 45 to 60% by weight, preferably 50 to 55% by weight, based on 100% by weight of the total antioxidant composition. If the content of the mulberry extract is less than the above range, there is little stabilizing effect of the antioxidant substance, and conversely, if it exceeds the above range, problems may occur during product processing.

The antioxidant composition according to the present invention includes the above-described mulberry extract and at least one antioxidant selected from the group consisting of epigallocatechin gallate, lutein, and astaxanthin.

The epigallocatechin gallate, [(2 R ,3 R )-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)chroman-3-yl] 3,4,5-trihydroxybenzoate ) is a type of catechin obtained from green tea, and has a strong antioxidant effect as a component of tannin or polyphenol, and is expressed in the following formula (1).

[Formula 1]

The epigallocatechin gallate can be obtained directly from green tea extract or a commercially available product can be used, but is not limited thereto.

The epigallocatechin gallate may be included in an amount of 2 to 5% by weight, preferably 3 to 4% by weight, based on 100% by weight of the total antioxidant composition. If the content of epigallocatechin gallate is less than the above range, the antioxidant effect is minimal, and conversely, if the content exceeds the above range, it may cause side effects on the human body or be difficult to dissolve uniformly, making it impossible to mix it in the formulation.

The lutein (β, ε-carotene-3,3′-diol) is a type of carotenoid pigment that exhibits antioxidant efficacy and is expressed in the following formula (2). The lutein can be obtained from marigold flowers.

[Formula 2]

The lutein can be obtained directly from marigold flower extract or commercially sold, but is not limited thereto.

The lutein may be included in an amount of 30 to 40% by weight, preferably 34 to 38% by weight, based on 100% by weight of the total antioxidant composition. If the content of lutein is less than the above range, the antioxidant effect is weak, and conversely, if the content of lutein exceeds the above range, it may cause side effects to the human body or adversely affect formulation.

The astaxanthin (3,3'-dihydroxy-β,β'-carotene-4,4'-dione) is a type of carotenoid pigment with the same chemical structure as beta-carotene, It is an antioxidant functional substance that eliminates harmful reactive oxygen species. Compared to beta-carotene, it has a unique molecular structural characteristic of having an extra hydroxyl group (-OH) and a ketone group (=O) at both ends, making it a much higher antioxidant than existing antioxidant substances. It has activity. The astaxanthin has an antioxidant activity that is 500 times higher than that of vitamin E, a representative antioxidant, and 20 times higher than that of beta-carotene, and is expressed in Chemical Formula 3 below.

[Formula 3]

The astaxanthin is produced in the yeast strain Phaffia rthodozyma and the algae Haematococcus and Bervibacterium , and is widely distributed in marine and freshwater animals. The astaxanthin can be extracted directly or purchased commercially and used, but is not limited thereto.

The astaxanthin may be included in an amount of 6 to 10% by weight, preferably 6 to 8% by weight, and more preferably 7 to 7.5% by weight, based on 100% by weight of the total antioxidant composition. If the content of astaxanthin is less than the above range, the antioxidant effect is weak, and conversely, if the content of astaxanthin exceeds the above range, it may cause side effects to the human body or cause problems in formulation.

The antioxidant composition of the present invention containing the above-described composition not only has excellent antioxidant effect, but also maintains the effect continuously by ensuring the stability of antioxidant substances, is easy to store and process, and has advantages in commercialization and industrialization.

Additionally, the present invention provides a pharmaceutical composition containing the antioxidant composition.

A pharmaceutical composition containing the antioxidant composition of the present invention may contain a pharmaceutically acceptable carrier. The composition containing a pharmaceutically acceptable carrier may be in various oral or parenteral dosage forms. When formulated, it can be prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain one or more compounds and at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. It can be prepared by mixing. In addition to simple excipients, lubricants such as magnesium stearate, talc, etc. may also be used. Liquid preparations for oral administration include suspensions, oral solutions, emulsions, and syrups. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. there is. Preparations for parenteral administration may include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, witepsol, macrogol, tween61, cacao, laurin, glycerogelatin, etc. can be used.

The pharmaceutical composition may have one or more dosage forms selected from the group consisting of powders, granules, tablets, capsules, suspensions, emulsions, syrups, solutions, aerosols, extracts, injections, transdermal administration, and suppositories.

The pharmaceutical composition is administered in a pharmaceutically effective amount. In this case, “pharmaceutically effective amount” means an amount sufficient to treat the disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type and severity of the individual, age, gender, activity of the drug, and the drug. It can be determined based on factors including sensitivity, time of administration, route of administration and excretion rate, duration of treatment, concurrently used drugs, and other factors well known in the medical field. For example, the antioxidant composition of the present invention may be administered in an amount of 0.001 to 100 mg/kg per day, preferably 1 to 10 mg/kg.

The pharmaceutical composition may be administered as an individual therapeutic agent or in combination with other therapeutic agents that exhibit antioxidant or anti-aging effects, and may be administered sequentially or simultaneously with conventional therapeutic agents. And it can be administered single or multiple times. Considering all of the above factors, it is important to administer an amount that can achieve maximum effect with the minimum amount without side effects, and can be easily determined by a person skilled in the art.

The pharmaceutical composition may be administered through any general route as long as it can reach the target tissue. The composition of the present invention may be administered intraperitoneally, intravenously, intramuscularly, subcutaneously, intradermally, orally, intranasally, intrapulmonaryly, or rectally, depending on the purpose, but is not limited thereto. Additionally, the composition may be administered by any device capable of transporting the active agent to target cells.

Additionally, the present invention provides a food composition containing the antioxidant composition. Examples of the food composition of the present invention include food, food additives, beverages, or beverage additives.

A food composition containing the antioxidant composition as an active ingredient may further include appropriate carriers, excipients, and diluents commonly used in its production.

The food composition may have one or more dosage forms selected from the group consisting of tablets, granules, powders, drinks, tea, caramels, jellies, and bars.

In the present invention, food refers to a natural product or processed product containing one or more nutrients, and preferably means that it has undergone a certain degree of processing to become ready to eat. In the usual sense, food, It is intended to include all food additives, health functional foods and beverages.

Foods to which the antioxidant composition of the present invention can be added include, for example, various foods, beverages, gum, candy, tea, vitamin complexes, functional foods, etc. Additionally, foods in the present invention include special nutritional foods (e.g., infant formula, infant/toddler food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g., ramen, noodles, etc.), health supplements, and seasoned foods. (e.g. soy sauce, soybean paste, red pepper paste, mixed paste, etc.), sauces, confectionery (e.g. snacks), dairy products (e.g. fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, pickles, etc.), beverages This includes, but is not limited to, fruits, vegetable drinks, soy milk, fermented drinks, ice cream, etc.), seasonings (e.g. ramen soup, etc.), vitamin complexes, alcoholic beverages, alcoholic beverages, and other health supplements. The food, beverage or food additive can be manufactured by conventional manufacturing methods.

In the present invention, health functional food refers to a food group or food composition in which added value is added to the food to act and express the function of the food for a specific purpose using physical, biochemical, biotechnological methods, etc., to control the biological defense rhythm, prevent diseases, and It refers to a food designed and processed to sufficiently express body regulatory functions related to recovery, etc. to the living body. Preferably, the functional food of the present invention refers to a food that can enhance antioxidant effects. The functional food may include food auxiliary additives that are foodologically acceptable, and may further include appropriate carriers, excipients, and diluents commonly used in the production of functional foods.

In the present invention, beverage refers to a general term for drinking to quench thirst or enjoy taste, and is intended to include functional beverages. In addition to containing the antioxidant composition as an essential ingredient in the indicated ratio, the beverage has no particular restrictions on other ingredients, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a regular beverage. Examples of the above natural carbohydrates include common sugars such as monosaccharides, such as glucose and fructose, disaccharides such as maltose, sucrose, etc., and polysaccharides, such as dextrins, cyclodextrins, etc., and Sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g., rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The ratio of the natural carbohydrate is generally about 1 to 20 g, preferably 5 to 12 g, per 100 ml of the composition of the present invention. In addition, the composition of the present invention is used as pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. It may additionally contain.

In addition to the above, the food composition of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and It may contain its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. These ingredients can be used independently or in combination.

In the present invention, a functional beverage refers to a group of beverages that have been given added value to function and express the function of the beverage for a specific purpose using physical, biochemical, or biotechnological techniques, or a beverage composition that includes biological defense rhythm regulation, disease prevention, and other functions. It refers to a beverage designed and processed to fully express body regulation functions related to recovery, etc. to the living body.

The functional beverage has no particular restrictions on other ingredients other than containing the antioxidant composition of the present invention as an essential ingredient in the indicated ratio, and may contain various flavoring agents or natural carbohydrates as additional ingredients like a conventional beverage. Examples of the natural carbohydrates include monosaccharides, such as glucose, fructose, etc., disaccharides, such as maltose, sucrose, etc., and polysaccharides, such as common sugars such as dextrin, cyclodextrin, etc., and xylitol. Sugar alcohols such as sorbitol and erythritol. As flavoring agents other than those mentioned above, natural flavoring agents (thaumatin, stevia extract (e.g. rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. You can.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Manufacturing example : Preparation of mulberry extract

[Production Example 1]

After drying the mulberries at room temperature, 10 times the volume of 70% ethanol was added, extracted for 4 hours at room temperature, and then filtered. The obtained filtrate was removed from the extraction solvent using a reduced pressure concentrator, sterilized at 95°C for 30 minutes, and then dried to prepare a mulberry extract.

[Production Example 2]

A mulberry extract was prepared in the same manner as Preparation Example 1, except that the same amount of purified water was used as the extraction solvent.

[Production Example 3]

A mulberry extract was prepared in the same manner as Preparation Example 1, except that the same amount of 30% ethanol was used as the extraction solvent.

[Production Example 4]

A mulberry extract was prepared in the same manner as in Preparation Example 1, except that the same amount of 50% ethanol was used as the extraction solvent.

[Production Example 5]

A mulberry extract was prepared in the same manner as in Preparation Example 1, except that the same amount of 100% ethanol was used as the extraction solvent.

Experiment example One. cyanidin -Measurement of 3-glucoside content

The content of cyanidin-3-glucoside (C3G) contained in the mulberry extract prepared in Preparation Examples 1 to 4 was measured.

Specifically, the mulberry extract was dissolved in a mixture of methanol and hydrochloric acid (49:1 (volume ratio)), and then a mixture of phosphoric acid and water (177:23 (volume ratio)) was added as a sample solution and used as a high-performance liquid chromatography (HPLC) method. analyzed. The content of the sample was determined by concentration gradient method using cyanidin-3-glucoside standard product. The results obtained at this time are shown in Table 1 below.

Manufacturing Example 1 Production example 2 Production example 3 Production example 4 Production example 5 C3G content
(%) 0.41 0.175 0.275 0.38 0.12

As shown in Table 1, it can be seen that when 70% alcohol was used as the extraction solvent, the content of cyanidin-3-glucoside in the mulberry extract was higher than when alcohols with different contents, including purified water, were used.

Example and Comparative example : Preparation of antioxidant composition

An antioxidant composition was prepared with the composition and content shown in Table 2 below.

Composition (% by weight) Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 EGCG ^{1 )} 3.6 100 - - Lutein ^{2 )} 35.7 - 100 - Astaxanthin ^{3 )} 7.2 - - 100 Mulberry extract of Preparation Example 1 53.5 - - - 1) EGCG: E4268, manufactured by Sigma Aldrich
2) Lutein: 07168, manufactured by Sigma Aldrich
3) Astaxanthin: SML0982, manufactured by Sigma Aldrich

Experiment example 2. Stability evaluation

The antioxidant compositions prepared in the above Examples and Comparative Examples were stored in an acceleration chamber with a temperature of 60°C and a humidity of 80% for 6 weeks, and changes in the content of each antioxidant were measured on a weekly basis. The obtained results are shown in Table 3 and Figures 1 to 3 below, and are the relative values when the total content of each antioxidant immediately after production is set to 100%.

Specifically, EGCG was extracted with methanol and quantitatively analyzed using a liquid chromatograph, and lutein was dispersed in distilled water, extracted with ethyl acetate, and the diluted solution with ethanol was analyzed using a high-performance liquid chromatograph and an ultraviolet absorbance photometric detector. Quantitative analysis was performed by measuring the absorbance at 446 nm, the maximum absorption wavelength. Astaxanthin was extracted with acetone, and then the ester group was separated and purified. The solution was quantitatively analyzed by measuring the absorbance at 474 nm, the maximum absorption wavelength, using a liquid chromatograph, an ultraviolet absorbance photodetector, and a normal phase column.

antioxidants content(%) 0 weeks 1 week 2 weeks 3 weeks 4 weeks EGCG Example 1 100 97.2 94.1 90.8 87.6 Comparative Example 1 100 85.4 62.7 44.2 31.3 lutein Example 1 100 96.5 93.7 90.8 85.3 Comparative Example 2 100 83.7 65.4 50.1 34.6 Astaxanthin Example 1 100 98.6 95.7 91.4 87.6 Comparative Example 3 100 81.2 66.2 48.2 36.7

As shown in Table 3 and Figure 1, in the case of the antioxidant composition containing the mulberry extract according to the present invention, the amount of reduction in antioxidant substances is significantly reduced compared to the composition containing each antioxidant substance alone without the mulberry extract. can confirm.

Looking at each component, it can be seen that in the case of EGCG, after one week of storage, the reduction rate was 14.6% in Comparative Example 1, while the stability was improved to 2.8% in Example 1. In the case of lutein, after 1 week of storage, the reduction rate was 16.3% in Comparative Example 2, while Example 1 showed improved stability of 3.5%. In addition, in the case of astaxanthin, it can be seen that the reduction rate was 18.8 in Comparative Example 3 after 1 week of storage, but the stability was improved to 1.4% in Example 1.

In addition, in the case of Comparative Examples 1 to 3 containing each antioxidant substance alone, the content continued to decrease after 1 week of storage, and the content of antioxidant substances fell to 1/3 of the level immediately after production, but in Example 1, the content continued to decrease after 1 week of storage. The content of antioxidant substances was more than 85% even after 4 weeks of storage, confirming that the stability of antioxidant substances was greatly improved.

Formulation example 1. Manufacturing of soft capsules (pharmaceutical preparations)

Soft capsules containing the antioxidant composition of the present invention were prepared by a conventional method according to the composition and content in Table 4 below.

ingredient soft capsule
(Unit: weight%) mulberry extract 26.75 Epigallocatechin gallate 1.80 lutein 17.85 Astaxanthin 3.60 beeswax 4.00 Soy lecithin 1.00 soybean oil 45.00

Formulation example 2. Manufacturing of liquid health functional food (food)

A liquid health functional food containing the antioxidant composition of the present invention was prepared by a conventional method according to the composition and content in Table 5 below.

ingredient Liquid health functional food (unit: weight %) mulberry extract 10.70 Epigallocatechin gallate 0.72 lutein 7.14 Astaxanthin 1.44 xanthan gum 10.00 Indigestible maltodextrin 25.00 Maltooligosaccharide 15.00 pear concentrate 20.00 Purified water 10.00

Claims (12)

Contains mulberry extract, epigallocatechin gallate, lutein, and astaxanthin;
The mulberry extract is contained in an amount of 45 to 60% by weight based on 100% by weight of the composition,
The epigallocatechin gallate is included in an amount of 2 to 5% by weight based on 100% by weight of the composition,
The lutein is included in an amount of 30 to 40% by weight based on 100% by weight of the composition,
The astaxanthin is contained in an amount of 6 to 10% by weight based on 100% by weight of the composition, a health functional food composition for antioxidant use. According to paragraph 1,
The mulberry extract is an antioxidant health functional food composition containing cyanidin-3-glucoside. According to paragraph 1,
A health functional food composition for antioxidants, wherein the mulberry extract is one or more extracts selected from the group consisting of water and organic solvents. According to paragraph 3,
The organic solvent is an antioxidant health agent containing at least one selected from the group consisting of C1 to C6 alcohols, acetone, ether, diethyl ether, ethyl acetate, ethyl methyl ketone, chloroform, glycerin, propylene glycol, and butylene glycol. Nutraceutical composition. delete delete delete delete delete delete delete According to any one of claims 1 to 4,
The composition is an antioxidant health functional food composition that is one or more dosage forms selected from the group consisting of tablets, granules, powders, drinks, tea, caramels, jellies, and bars.