KR102642811B1 - Composition for relieving hangover based on soybean curd residue micro-powder - Google Patents

Abstract

The present disclosure discloses a composition for relieving hangovers based on okara fine powder. The composition contains turmeric extract powder, okara powder, yeast extract powder, corn starch, and milk thistle powder, which has the effect of reducing alcohol content in the blood and enhancing the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). is excellent.

Description

Composition for relieving hangover based on okara fine powder {COMPOSITION FOR RELIEVING HANGOVER BASED ON SOYBEAN CURD RESIDUE MICRO-POWDER}

The present disclosure discloses a composition for relieving hangovers based on okara fine powder.

The hangover phenomenon is caused by the action of alcohol and acetaldehyde accumulated in liver cells and the body. When a small amount of alcohol is consumed under normal conditions, the ethanol that enters the liver and intestines is converted to acetate by the action of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in the cell cytosol. converted to , which is excreted out of the liver cells through the circulatory system. Among these, acetaldehyde, the first metabolite of ethanol, is known to be a major cause of alcoholic liver failure as it is highly reactive and highly toxic compared to ethanol.

KR 10-0903690 B1

In one aspect, the purpose of the present disclosure is to provide a composition for relieving hangovers based on okara fine powder.

In one aspect, the present disclosure provides a composition for relieving a hangover containing turmeric and okara as active ingredients.

In an exemplary embodiment, the composition may include 2 to 5% by weight and 25 to 40% by weight of turmeric and okara, respectively, based on the total weight of the composition.

In an exemplary embodiment, the composition may further include yeast, corn starch, and milk thistle.

In an exemplary embodiment, the composition may contain 25 to 40% by weight, 15 to 25% by weight, and 3 to 6% by weight of yeast, corn starch, and milk thistle, respectively, based on the total weight of the composition.

In an exemplary embodiment, the composition may include turmeric extract powder, okara powder, yeast extract powder, corn starch, and milk thistle extract powder.

In an exemplary embodiment, the composition includes turmeric extract powder, okara powder, yeast extract powder, corn starch, and milk thistle extract powder in a weight ratio of 1:8 to 10:8 to 10:6 to 8:1 to 2. It may include

In one exemplary embodiment, the composition may reduce alcohol content in the blood.

In an exemplary embodiment, the composition may enhance the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).

In another aspect, the present disclosure provides a health supplement for relieving hangovers, including a composition for relieving hangovers.

In an exemplary embodiment, the health supplement may have a liquid, pill, tablet, granule, powder, or capsule formulation.

In one aspect, the technology disclosed in the present disclosure has the effect of providing a composition for relieving hangovers based on okara fine powder.

Figure 1 shows the pretreatment process for HPLC analysis of a mixture of curcumin complex and okara according to an experimental example.
Figure 2 shows a photo of pretreatment for HPLC analysis of a mixture of curcumin complex and okara according to an experimental example.
Figure 3 shows a 440 nm chromatogram as a result of HPLC analysis according to an experimental example.
Figure 4 shows the results of in vitro alcohol dehydrogenase (ADH) enzyme activity measurement according to an experimental example.
Figure 5 shows the results of in vitro aldehyde dehydrogenase (ALDH) enzyme activity measurement according to an experimental example.
Figure 6 shows the results of in vivo blood alcohol concentration measurement according to an experimental example.
Figure 7 shows the results of in vivo alcohol dehydrogenase (ADH) enzyme activity measurement according to an experimental example.
Figure 8 shows the results of measuring in vivo aldehyde dehydrogenase (ALDH) enzyme activity according to an experimental example.

Hereinafter, the present disclosure will be described in detail.

In one aspect, the present disclosure provides a composition for relieving a hangover containing turmeric and okara as active ingredients.

Turmeric ( Curcuma longa ) is a plant belonging to the ginger family. The rhizome used for medicinal purposes contains various useful ingredients, the most representative ingredient being curcumin. The turmeric is a rhizome of turmeric, and in an exemplary embodiment, the turmeric may be a powder, extract, or powder of an extract of turmeric.

The turmeric extract can be manufactured by direct extraction according to a method known in the art, or it can be used by purchasing a commercially available product. In an exemplary embodiment, the turmeric extract is water, alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, butanol, etc.), ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, One or more solvents selected from the group consisting of N, N-dimethylformamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerin, butylene glycol, propylene glycol, dipropylene glycol, methylene chloride, and diethyl ether. It may have been extracted.

Bean curd refers to the by-product left over from squeezing soy milk when making tofu. For example, the soybean curd refers to a by-product remaining after extracting soymilk from soybean protein, and may be obtained by removing the water-soluble protein from the soybean protein. The soy protein refers to the by-product remaining after removing the oil from the soybean, and the milking process can be performed by a person skilled in the art by appropriately selecting a method known in the art. For example, the soy protein may be a compressed soy protein, which is a by-product remaining after oil is removed from soybeans by pressing without using an organic solvent, for example, by pressing at room temperature.

Although a large amount of water-soluble substances have been removed from the soybean curd, it contains nutrients necessary for the human body, including high-quality protein and abundant fiber. It is an excellent protein resource with the highest protein efficiency ratio (PER), which is a value representing the ratio of weight gain based on protein intake, among soybeans, soymilk, and tofu. In addition, okara is high in isoflavones (phytoestrogens), which act similarly to the female hormone estrogen, so it can be used to prevent and treat menopausal syndrome.

In an exemplary embodiment, the soybean curd may be dried and ground.

In one exemplary embodiment, the soybean curd may be obtained by drying by-products remaining after manufacturing tofu with hot air and then powdering them into fine powder.

In one exemplary embodiment, the soybean curd may have a size of 500 to 2,000 mesh. In another exemplary embodiment, the soybean curd is 500 mesh or more, 600 mesh or more, 700 mesh or more, 800 mesh or more, 900 mesh or more, or 1,000 mesh or more, 2,000 mesh or less, 1,900 mesh or less, 1,800 mesh or less, 1,700 mesh. It may have a size of 1,600 mesh or less, 1,500 mesh or less, 1,400 mesh or less, 1,300 mesh or less, 1,200 mesh or less, 1,100 mesh or less, or 1,000 mesh or less. For example, it may be desirable for the soybean curd to have a size of 900 to 1,100 mesh or 1,000 mesh.

In an exemplary embodiment, the composition may include 2 to 5% by weight and 25 to 40% by weight of turmeric and okara, respectively, based on the total weight of the composition.

In an exemplary embodiment, the composition contains 2% by weight or more, 3% by weight or more, or 4% by weight or more, and 5% by weight or less, 4% by weight or less, or 3% by weight or less of turmeric based on the total weight of the composition. It may be.

In an exemplary embodiment, the composition contains at least 25% by weight, at least 28% by weight, at least 31% by weight, or at least 34% by weight, and at least 40% by weight, at most 37% by weight, at least 34% by weight, based on the total weight of the composition. It may contain less than % by weight or less than 31% by weight.

In an exemplary embodiment, the composition may further include yeast, corn starch, and milk thistle.

Yeast is a microorganism used to make bread, beer, wine, etc. It is a group of molds or mushrooms, but it is a general term for single-celled organisms that do not have hyphae and do not have photosynthesis or motility. In an exemplary embodiment, the yeast may be dry yeast powder, yeast extract, or yeast extract powder.

The dry yeast powder refers to the powder of dry yeast. Dry yeast refers to yeast that has been dried to enable storage because yeast has a lot of moisture and is easily perishable. Dry yeast must be dried to prevent the yeast from dying, and in an exemplary embodiment, drying by ventilation at a low temperature, drying at a temperature of about 30° C. for 8 hours, and drying by ventilation in a drying tank rotating in a horizontal direction. Yeast can be dried according to methods known in the art, such as methods known in the art, and freeze-drying may be preferable to suppress death of yeast due to heat.

The yeast extract can be prepared by directly extracting yeast from yeast according to a method known in the art, or it can be used by purchasing a commercially available product. In an exemplary embodiment, the preparation of the yeast extract is performed by first adding water equivalent to 5 to 10 times the weight of the yeast to dry yeast, cultivating the yeast at room temperature for 1 to 5 hours, and then activating the yeast at 50 to 88 ° C. A yeast extract can be obtained by extracting for 0.5 to 5 hours and then filtering. In addition, the obtained yeast extract was concentrated to a solid content of 10% (w/v) or more, the concentrated yeast extract was filtered using an ultrafiltration membrane in a system equipped with a filtration membrane with a molecular weight of 5,000 to 20,000 daltons, and then concentrated again. Yeast extract can be obtained by freeze-drying.

The yeast extract powder refers to a powder of yeast extract.

In one exemplary embodiment, the corn starch may be corn starch powder.

Milk thistle ( Silybum marianum ) is a plant in the Asteraceae family. Its fruits and seeds contain flavonolignans, and its major components are collectively called silymarin. Silymarin is known to help liver health. In an exemplary embodiment, the milk thistle may be milk thistle powder, milk thistle extract, or milk thistle extract powder. The milk thistle extract powder refers to the powder of milk thistle extract. In an exemplary embodiment, the milk thistle may be a powder, extract, or powder of an extract of milk thistle seeds.

The milk thistle extract can be manufactured by direct extraction according to a method known in the art, or it can be used by purchasing a commercially available product. In an exemplary embodiment, the milk thistle extract is water, alcohol having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, butanol, etc.), ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloroform. At least one selected from the group consisting of methane, N, N-dimethylformamide (DMF), methylene chloride, dimethyl sulfoxide (DMSO), glycerin, butylene glycol, propylene glycol, dipropylene glycol, methylene chloride, and diethyl ether. It may have been extracted with a solvent.

In an exemplary embodiment, the composition may contain 25 to 40% by weight, 15 to 25% by weight, and 3 to 6% by weight of yeast, corn starch, and milk thistle, respectively, based on the total weight of the composition.

In an exemplary embodiment, the composition contains at least 25% by weight, at least 28% by weight, at least 31% by weight, or at least 34% by weight, and at least 40% by weight, at most 37% by weight, at least 34% by weight, based on the total weight of the composition. It may contain less than % by weight or less than 31% by weight.

In an exemplary embodiment, the composition contains 15% by weight or more, 18% by weight or more, or 21% by weight or more, and 25% by weight or less, 23% by weight or less, or 21% by weight or less, based on the total weight of the composition. It may contain less than 19% by weight.

In an exemplary embodiment, the composition contains 3% by weight or more, 4% by weight or more, or 5% by weight or more, and 6% by weight or less, 5% by weight or less, or 4% by weight or less, based on the total weight of the composition. It may be included.

In an exemplary embodiment, the composition may include turmeric extract powder, okara powder, yeast extract powder, corn starch, and milk thistle extract powder.

In an exemplary embodiment, the composition includes turmeric extract powder, okara powder, yeast extract powder, corn starch, and milk thistle extract powder in a weight ratio of 1:8 to 10:8 to 10:6 to 8:1 to 2. It may include

In one exemplary embodiment, the composition may reduce alcohol content in the blood.

In an exemplary embodiment, the composition may enhance the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).

In another aspect, the present disclosure provides a health supplement for relieving hangovers, including a composition for relieving hangovers.

In an exemplary embodiment, the health supplement may have a liquid, pill, tablet, granule, powder, or capsule formulation.

Hereinafter, the present disclosure will be described in more detail through examples. It will be apparent to those skilled in the art that these examples are solely for illustrating the present disclosure, and that the scope of the present disclosure should not be construed as limited by these examples.

Example. Manufacture of composition for relieving hangover

A composition for relieving hangovers was prepared according to the composition ratio in Table 1 below and used in the following experimental examples. In addition, EASY TOMORROW was purchased from Q1 Co., Ltd. and used as a positive control in Example 3.

Raw material Example 1 (% by weight) Example 2 (% by weight) yeast extract 30 30 Soybean curd powder 0 29 corn starch 50 21 Milk Thistle Extract Powder 4 4 Turmeric Extract Powder 3 3 vitamin mix 3 3 glutinous rice powder 3 3 Hydroxypropylmethylcellulose 3 3 crystalline cellulose 1.9 1.9 L-Glutamine One One glycine One One Drink scent 0.1 0.1 Sum 100 100

Experimental Example 1. Component analysis of curcumin complex and okara mixture

Curcumin complex (Curcumin) containing 70 to 80% by weight of curcumin, the main component of turmeric, 15 to 25% by weight of demethoxycurcumin (DMC), and 2.5 to 6.5% by weight of bisdemethoxy curcumin (BDMC) C3 complex) and the components of the okara mixture obtained by adding soybean curd powder to the curcumin complex were analyzed and compared.

In the above equation,

Curcumin is R ₁ =OCH ₃ , R ₂ =OCH ₃ , MW=368,

Dimethoxycurcumin has R ₁ =OCH ₃ , R ₂ =H, MW=338,

Bisdimethoxy curcumin has R ₁ =H, R ₂ =H, MW=308.

Specifically, three types of samples were prepared as follows.

(1) Curcumin C3 complex (20 mg) + H ₂ O (200 mL)

(2) Okara powder (200 mg) + H ₂ O (200 mL)

(3) Okara mixture (Curcumin C3 complex (20 mg) + Okara powder (200 mg)) + H ₂ O (200 mL)

After stirring the three samples prepared above for 30 minutes at 35°C, 1 mL of the emulsion was taken, diluted with 2 mL of MeOH, and then analyzed by HPLC. In addition, the three samples prepared above were stirred at 35°C for 30 minutes, then homogenized with an ultrasonic homogenizer at 6,000 rpm for 2 minutes, and 1 mL of the emulsion was taken, diluted with 2 mL of MeOH, and then analyzed by HPLC (Figure 1 and 2). The HPLC analysis conditions are as follows, and the results are shown in Figure 3.

HPLC analysis device name: Agilent 1200 series

- Degasser: G1322A

- Pump: G1312A

- Autosampler: G1329A

- Column oven: G1316A

-Detector(DAD): G1315B

Solvent: 0.1% FA in ACN(A), 0.1% FA in Water(B)

Slope: A: 0min(10%)-5min(10%)-40min(100%)-45min(100%)

Column: YMC ODS-A C18/ 150x4.6mmI.D./ S-5um/ 12nm

Column oven: 30℃

Flow rate: 0.7ml/min

Injection amount: 10ul

HPLC monitored: 210, 254, 280, 330, 420 nm

As a result, it was confirmed that when okara powder was added to the curcumin complex, there was a significant difference in the chemical composition of the curcumin complex.

Experiment example 2. ADH and ALDH Enzyme activity assay ( in vitro )

ADH (Alcohol Dehydrogenase) and ALDH (Aldehyde Dehydrogenase), enzymes involved in alcohol metabolism, were purchased from Sigma Chemical Co. (Saint Louis, Mo., USA), lyophilized S9 rat liver homogenate was purchased from MolTox Co. It was purchased and used in (North Carolina, USA).

ADH enzyme activity was measured using the Alcohol Dehydrogenase Activity Assay Kit (sigma, #MAK053) according to the method suggested by the manufacturer. S9 rat liver fraction was dissolved in 0.1% bovine serum albumin solution, prepared at a protein concentration of 1 mg/mL, and used as an enzyme source. The test sample was reacted with the Reaction mix (ADH assay buffer, Developer, 2M Ethanol) included in the kit, and the change in absorbance was measured at 450 nm every hour for 3 hours at 37°C. The activity of ADH was calculated as the relative % activity of the maximum absorbance at the end of the reaction with respect to the maximum absorbance of the control group. At this time, the one in which no sample was added was used as the control group.

ALDH enzyme activity was measured using the Aldehyde Dehydrogenase Activity Colorimetric Assay Kit (sigma, #MAK082) according to the method suggested by the manufacturer. S9 rat liver fraction was dissolved in 0.1% bovine serum albumin solution, prepared at a protein concentration of 1 mg/mL, and used as an enzyme source. The test sample was reacted with the reaction mix (ALDH assay buffer, ALDH Substrate Mix, Acetaldehyde) included in the kit, and the change in absorbance was measured at 450 nm every hour for 3 hours at room temperature. ALDH activity was expressed as relative activity (%) to the control group. At this time, the one in which no sample was added was used as the control group.

As a result, ADH activity was 163% in Example 1, 178% in Example 2, and 172.1% in Example 3 at 3 hours. When the control was set to 100% as a control group without adding the sample, the ADH activity increased in all samples. In particular, ADH activity increased the most in the sample of Example 2 (see Figure 4).

In addition, when control was set to 100% without adding a sample as a control, ALDH activity was most promoted at 3 hours in Example 2 at 327%, and activity increased to 201% in Example 1 and 305.4% in Example 3. was confirmed (see Figure 5). It was found that the Example 2 sample had the effect of quickly decomposing acetaldehyde and further improving the hangover relief effect.

Experimental Example 3. Blood alcohol concentration, ADH and ALDH enzyme activity analysis ( in vivo )

(1) Laboratory animals

The animals used in this experimental example were 7-week-old Sprague-Dawley (SD) male rats weighing 260 to 280 g, purchased from BioOrient Co., Seoul, Korea, and fed with solid mixed feed for 1 week. After pre-rearing with water, the experiment was performed when the body weight reached 280 to 300 g. The breeding environment was maintained at a temperature of 25 ± 2°C and humidity of 50 ± 5%, and the animals were reared in individual cages with a light cycle maintained at 12-hour intervals. Sufficient food and water were provided during rearing.

(2) Providing samples and causing liver damage

The experimental group was divided into a total of 5 groups: normal group (control), alcohol administration group (negative control), Example 1 administration group, Example 2 administration group, and Example 3 administration group (Table 2). Alcohol absorption phenomenon was observed due to feed intake. To exclude this, the subjects were fasted for 18 hours before ethanol administration. The method of administration of each test sample was to prepare the test sample in D.W (Distilled water) at a concentration of 312 mg/Kg and administer it orally. After 30 minutes, alcohol was administered orally at an amount of 3 g/Kg, and blood was collected from the heart at 1, 3, and 5 hours. The collected blood was mixed by adding 4 M perchloric acid to a final concentration of 1 M, left at 4°C for 5 minutes, and centrifuged at 13,000 rpm for 2 minutes in refrigerated condition. 34 ul of 2M KOH was mixed with 100 ul of the supernatant, the pH was adjusted to between 6.5 and 8.0, and the mixture was left at 4°C for 30 minutes. After centrifugation at 15,000 rpm for 15 minutes in refrigerated condition to remove proteins, the ethanol concentration of the supernatant was measured using an ethanol measurement kit (Sigma, #MAK076).

experimental group process Control D.W. Negative control 3 g/Kg ethanol Example 1 (bean curd X) 312 mg/Kg sample + 3 g/Kg ethanol Example 2 (Kobiji o) 312 mg/Kg sample + 3 g/Kg ethanol Example 3 (Refreshing pill) 312 mg/Kg sample + 3 g/Kg ethanol

(3) Measurement of ADH and ALDH activity in liver tissue

The liver tissue was thawed and washed three times with D-PBS, then 10 times the tissue weight (g) of 0.1 M Tris-HCl buffer (pH 7.4) was added and homogenized using a grinder under ice cooling. ADH and ALDH activities of liver tissue homogenates were measured using assay kits (sigma, #MAK053, sigma, #MAK082) manufactured by colorimetrically measuring the amount of NADH produced by NAD+ reduction, and the It was presented in milliunits/mL using the calculation formula.

To confirm the effectiveness of improving hangovers, a single dose of alcohol was administered to rats, blood was collected 1, 3, and 5 hours later to measure blood alcohol concentration, liver was extracted, and ADH and ALDH concentrations were measured. The results are shown in Figures 6 to 8. indicated.

Consistent with previous reports that blood alcohol concentration peaks between 60 and 90 minutes after drinking, the blood alcohol concentration reached its peak in just one hour. There was no significant change in all samples at 1 hour after ethanol administration, but at 3 and 5 hours after ethanol administration, it was confirmed that the blood ethanol concentration of most samples decreased compared to the alcohol administration group (see Figure 6). In particular, the Example 2 sample reduced blood alcohol concentration more than the Example 1 sample at all times and showed the lowest blood alcohol concentration at 5 hours. Example 2 The sample showed a blood alcohol level that was about 0.8 times lower than that of the normal group.

ADH activity was highest at 3 hours, with the normal group (control, D.W) being 0.587±0.09 mU/mL and the experimental group being 2.687±0.82 mU/mL, 4.559±1.71 mU/mL, and 4.105±1.70 mU/mL, respectively. It was found that the samples of Examples 2 and 3 showed ADH activity that was about 7.7 and 6.9 times higher than that of the normal group (see Figure 7).

In addition, ALDH activity in liver tissue decreased due to ethanol administration, but ALDH activity increased from 2 hours upon administration of the test sample. Specifically, the experimental group showed ALDH activity of 0.923±0.235 mU/mL, 1.229±0.366 mU/mL, and 1.098±0.343 mU/mL, respectively, which was 5.4 times higher than the 0.170±0.024 mU/mL of the normal group (control, D.W). It showed 7.2- and 6.4-fold higher activity (see Figure 8). In particular, it was confirmed that the sample of Example 2 had the effect of quickly decomposing acetaldehyde and further enhancing the hangover relief effect. This was consistent with the results of promoting liver enzyme ALDH activity in vitro.

As described above, specific parts of the present disclosure have been described in detail, and it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present disclosure. something to do. Accordingly, the substantial scope of the present disclosure will be defined by the appended claims and their equivalents.

Claims (10)

Contains turmeric extract powder, okara powder, yeast extract powder, corn starch powder, and milk thistle extract powder as active ingredients,
A composition for relieving a hangover, wherein the okara powder has a size of 500 to 2,000 mesh.
According to clause 1,
The composition contains 2 to 5% by weight, 25 to 40% by weight, 25 to 40% by weight, and 15% of turmeric extract powder, okara powder, yeast extract powder, corn starch powder, and milk thistle extract powder, respectively, based on the total weight of the composition. A composition for relieving a hangover, comprising from 25% by weight and 3 to 6% by weight.
delete According to clause 1,
The composition contains 3 to 4% by weight, 28 to 31% by weight, 28 to 31% by weight, and 18 to 4% by weight of turmeric extract powder, okara powder, yeast extract powder, corn starch powder, and milk thistle extract powder based on the total weight of the composition. A composition for relieving a hangover, comprising 23% by weight and 3 to 4% by weight.
According to clause 1,
A composition for relieving a hangover, wherein the okara powder has a size of 900 to 1,100 mesh.
According to clause 1,
The composition includes turmeric extract powder, okara powder, yeast extract powder, corn starch powder, and milk thistle extract powder in a weight ratio of 1:8 to 10:8 to 10:6 to 8:1 to 2. Composition for resolving.
According to clause 1,
The composition is a composition for relieving a hangover, which reduces the alcohol content in the blood.
According to clause 1,
The composition is a composition for relieving a hangover, which improves the activity of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH).
A health supplement for relieving hangovers, comprising the composition for relieving hangovers according to any one of claims 1, 2, and 4 to 8.
According to clause 9,
The health supplement is a health supplement for relieving a hangover, having a liquid, pill, tablet, granule, powder, or capsule formulation.