KR102635365B1 - Necleic acid extraction device - Google Patents

Abstract

The present invention includes a seating portion 200 on which a cartridge 20 having one or more wells (W) is seated; One or more magnetic rods 400; A magnetic rod holder 500 coupled to the magnetic rod 400 and installed to be movable up and down on the seating portion 200; It is coupled to one or more caps (C), is disposed below the magnetic rod holder 500, and is installed to be movable up and down on the seating portion 200, so that the cap (C) is seated on the seating portion 200. A cap holder 600 that enters into or retreats from the inside of the well W of the cartridge 20; a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 or the seating unit 200 in a first direction crossing the vertical direction; A cover that enters or retreats into the space between the cartridge 20 seated in the seating portion 200 and the cap C coupled to the cap holder 600 with the cap C retracted from the inside of the well W. Provided is a nucleic acid extraction device comprising (1000) and a fourth driving unit (1100) that causes the cover (1000) to enter or retreat from the space. The cover 1000 and the fourth driving unit 1100 are installed to be movable in the first direction along the cap holder 600, and the cover 1000 is placed higher than the cartridge 20 seated on the seating unit 200. You can.

Description

Nucleic acid extraction device {NECLEIC ACID EXTRACTION DEVICE}

The present invention relates to a nucleic acid extraction device, and more specifically, to a nucleic acid extraction device that prevents foreign substances from entering a well.

A method of extracting nucleic acids or biological substances (proteins, etc.) from samples collected from organisms, viruses, etc. using magnetic beads is widely used.

In order to extract nucleic acids or biological substances using magnetic beads, several steps such as lysis & binding, washing, and elution must be performed, making the extraction process complex and time-consuming. . Accordingly, extraction devices that automatically perform the above various steps have been developed and used. Prior art related to this includes Korean Patent Publication No. 10-2020-0032639.

However, conventional nucleic acid extraction devices do not prevent foreign substances from entering wells placed along the movement path when moving magnetic beads to another well. Accordingly, the reliability of the extracted nucleic acid is lowered and the nucleic acid must be extracted again, which may lower the nucleic acid extraction efficiency.

In particular, when extracting a large amount of nucleic acid at once, a large amount of magnetic beads must be transferred to another cartridge or another well at the same time to perform the next step of nucleic acid extraction. In the process of moving magnetic beads, some of the large amount of magnetic beads may easily fall off, and substances such as cleaning fluid present in the space between the large amount of magnetic beads may fall. Accordingly, it may be impossible to correctly extract a large amount of nucleic acid with a conventional nucleic acid extraction device.

KR 10-2020-0032639

The present invention has been devised to solve the above-mentioned problems, and the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that prevents foreign substances from entering the well.

Additionally, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device with improved durability, stability, and nucleic acid extraction efficiency.

Additionally, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that can correctly and effectively extract a large amount of nucleic acid easily and at low cost with a simple configuration.

Additionally, the purpose of embodiments of the present invention is to provide a nucleic acid extraction device that can be manufactured and maintained easily at low cost and can be miniaturized and lightweight.

Additionally, embodiments of the present invention aim to provide a nucleic acid extraction device that is simple and stably controlled.

In order to solve the above-described problem, the present invention includes a seating portion 200 on which a cartridge 20 having one or more wells (W) is seated; One or more magnetic rods 400; A magnetic rod holder 500 coupled to the magnetic rod 400 and installed to be movable up and down on the seating portion 200; It is coupled to one or more caps (C), is disposed below the magnetic rod holder 500, and is installed to be movable up and down on the seating portion 200 to attach the cap (C) to the seating portion 200. A cap holder 600 that allows the cartridge 20 seated in the well W to enter or retreat from the well W; a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 or the seating unit 200 in a first direction crossing the vertical direction; In a state where the cap (C) is retracted from the inside of the well (W), it enters the space between the cartridge (20) seated on the seating portion (200) and the cap (C) coupled to the cap holder (600). A nucleic acid extraction device is provided, including a cover 1000 to be retracted and a fourth driving unit 1100 that allows the cover 1000 to enter or retract the space.

The cover 1000 and the fourth driving unit 1100 may be installed to be movable in a first direction along the cap holder 600.

The cover 1000 may be placed higher than the cartridge 20 seated on the seating portion 200.

In one embodiment, the fourth driving unit 1100 is disposed on one side of the cap holder 600 in a first direction and overlaps with the cap holder 600 in a vertical direction and a second direction intersecting the first direction. It may be placed higher than the cartridge 20 seated on the seating portion 200.

In one embodiment, the fourth driving unit 1100 may include a fourth motor 1110 that causes the cover 1000 to enter or retreat from the space.

The fourth motor 1110 may be installed to be movable in a first direction along the cap holder 600.

It is possible to prevent materials such as magnetic beads, cleaning fluid (e.g., ethanol), and samples that fall from the cap (C) from entering the well (W). In particular, even if the movement distance of the cap C in the first direction is long or vibration/shock occurs during movement, foreign matter can be prevented from entering the well W located at the bottom of the movement path. Accordingly, nucleic acid extraction efficiency can be improved.

In addition, when extracting a large amount of nucleic acid at once, a large amount of magnetic beads must be transferred to another cartridge 20 or another well (W) at once to perform the next step of nucleic acid extraction. At this time, some of the large amount of magnetic beads attached by magnetic force to the cap (C) into which the magnetic rod 400 is inserted can be easily separated, and substances such as cleaning fluid existing in the space between the large amount of magnetic beads are attached to the cap (C). C) may fall. In this way, even if a large amount of magnetic beads are attached to the cap (C) and moved all at once, the cover (1000) can prevent foreign substances from entering the well (W). Accordingly, with a simple structure, a large amount of nucleic acid can be extracted correctly and effectively at low cost and easily.

With a simple configuration, the material falling from the cap (C) can be easily collected at low cost. Accordingly, since there is no need for a separate configuration to collect materials falling from the cap C, manufacturing and maintenance can be facilitated at low cost.

Since the cover 1000 and the fourth driving unit 1100 can be placed and operated close to the main frame 300, vibration can be reduced and pressure applied to the main frame 300, etc. Accordingly, the durability, stability, and nucleic acid extraction efficiency of the nucleic acid extraction device can be improved.

Additionally, the cover 1000 can be included in the nucleic acid extraction device by simply providing an additional space the size of the cover 1000 in the first direction. For example, if the cover 1000 is designed to enter or retreat into the space between the cartridge 20 and the cap C in the first direction, the cover 1000 can operate in an existing nucleic acid extraction device as long as the additional space is secured. can do. Accordingly, the nucleic acid extraction device can be miniaturized and lightweight, and costs can be reduced.

When the position of the cap holder 600 in the first direction changes, the positions of the cover 1000 and the fourth driving unit 1100 in the first direction may also change in the same way. Accordingly, the cap holder 600, cover 1000, and fourth driving unit 1100 can be controlled more simply and stably than when they move in the first direction independently of each other.

In addition, due to a malfunction, such as misjudging the position (height) of the cap holder 600, the cover 1000 collides with the cap holder 600, etc., damaging the nucleic acid extraction device, or the cover 1000 moves to the wrong position and the well ( It is possible to prevent foreign substances from entering W).

In addition to the above-described effects, specific effects of the present invention are described below while explaining specific details for carrying out the invention.

Figures 1 and 2 are perspective views showing a nucleic acid extraction device according to an embodiment of the present invention, and Figure 3 is a state diagram showing a state in which the door is opened and the seating portion is withdrawn from the nucleic acid extraction device of Figure 1.
Figures 4 and 5 are perspective views showing the interior of a nucleic acid extraction device according to an embodiment of the present invention.
Figure 6 is a state diagram showing a state in which the seating portion is withdrawn from the nucleic acid extraction device of Figure 4.
Figure 7 is a perspective view showing a state in which the cartridge is seated in the seating portion of the nucleic acid extraction device of Figure 4.
Figure 8 is a perspective view showing a state in which a cap is coupled to a cap holder in the nucleic acid extraction device of Figure 7.
Figure 9 is a perspective view of a cartridge having multiple (6 x 4) wells according to one embodiment of the present invention.
10 and 11 are perspective views showing a magnetic rod holder combined with a magnetic rod according to an embodiment of the present invention.
12 to 14 are perspective views, plan views, and front views of a cap holder equipped with a fixing device according to an embodiment of the present invention, showing the insertion hole of the cap holder in an open state.
Figures 15 to 17 are a perspective view, plan view, and front view of a cap holder equipped with a fixing device according to an embodiment of the present invention, showing the insertion hole of the cap holder in a closed state.
Figure 18 is an exploded perspective view of Figure 12.
Figure 19 is a cross-sectional view of the rotating part of the fixture.
Figure 20 is a perspective view of a strip according to an embodiment of the present invention.
Figure 21 is a diagram showing a state in which the strip of Figure 20 is inserted into the cap holder, the cap of the strip is inserted into the well of the cartridge, and the magnetic rod coupled to the magnetic rod holder is inserted into the cap.
Figures 22 and 23 are perspective views showing main components related to the second driving unit according to an embodiment of the present invention.
Figures 24 and 25 are exploded perspective views of Figures 22 and 23.
Figures 26 and 27 are perspective views showing main components related to the first driving unit according to an embodiment of the present invention.
Figures 28 and 29 are exploded perspective views of Figures 26 and 27.
Figures 30 and 31 are perspective views showing main components related to the third driving unit according to an embodiment of the present invention.
Figures 32 and 33 are exploded perspective views of Figures 30 and 31.
Figures 34 and 35 are perspective views showing the main frame, support frame, cover, and fourth driving unit according to an embodiment of the present invention.
Figures 36 and 37 are exploded perspective views of Figures 34 and 35.
Figures 38 to 55 are perspective and front views showing the process of moving magnetic beads using a nucleic acid extraction device according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention is not limited to the embodiments disclosed below, but may be subject to various changes and may be implemented in various different forms. This example is provided solely to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the scope of the invention. Therefore, the present invention is not limited to the embodiments disclosed below, but substitutes or adds to the configuration of one embodiment and the configuration of another embodiment, as well as all changes and equivalents included in the technical spirit and scope of the present invention. It should be understood to include substitutes.

The attached drawings are only for easy understanding of the embodiments disclosed in this specification, and the technical idea disclosed in this specification is not limited by the attached drawings, and all changes, equivalents, and changes included in the spirit and technical scope of the present invention are not limited. It should be understood to include water or substitutes. In the drawings, components may be expressed exaggeratedly large or small in size or thickness for convenience of understanding, etc., but the scope of protection of the present invention should not be construed as limited due to this.

The terms used in this specification are only used to describe specific implementations or examples, and are not intended to limit the invention. And singular expressions include plural expressions, unless the context clearly dictates otherwise. In the specification, terms such as ~include, ~consist of, etc. are intended to indicate the existence of features, numbers, steps, operations, components, parts, or a combination thereof described in the specification. In other words, terms such as ~include, ~consist, etc. in the specification. It should be understood that this does not exclude in advance the presence or addition of one or more other features, numbers, steps, operations, components, parts or combinations thereof.

Terms containing ordinal numbers, such as first, second, etc., may be used to describe various components, but the components are not limited by the terms. The above terms are used only for the purpose of distinguishing one component from another.

When a component is said to be "connected" or "connected" to another component, it is understood that it may be directly connected to or connected to the other component, but that other components may exist in between. It should be. On the other hand, when it is mentioned that a component is “directly connected” or “directly connected” to another component, it should be understood that there are no other components in between.

When a component is referred to as being "on top" or "below" another component, it should be understood that not only is it placed directly on top of the other component, but there may also be other components in between. .

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless explicitly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The nucleic acid extraction device disclosed in the following examples will be examined in more detail with reference to each drawing.

Figures 1 and 2 are perspective views showing a nucleic acid extraction device according to an embodiment of the present invention, and Figure 3 is a state diagram showing a state in which the door is opened and the seating portion is withdrawn from the nucleic acid extraction device of Figure 1. Figures 4 and 5 are perspective views showing the interior of a nucleic acid extraction device according to an embodiment of the present invention. Figure 6 is a state diagram showing a state in which the seating portion is withdrawn from the nucleic acid extraction device of Figure 4. Figure 7 is a perspective view showing a state in which the cartridge is seated in the seating portion of the nucleic acid extraction device of Figure 4. Figure 8 is a perspective view showing a state in which a cap is coupled to a cap holder in the nucleic acid extraction device of Figure 7.

Referring to FIGS. 1 to 8, the nucleic acid extraction device 10 according to one embodiment includes a base 100, a seating portion 200, a basic frame (FF), a main frame 300, a magnetic rod 400, Magnetic rod holder 500, cap holder 600, first driving part 700, second driving part 800, third driving part 900, cover 1000, fourth driving part 1100, control part 1200, 5), it may include a housing 1300, a door 1400, a display unit 1500, and an input unit 1600.

[Base]

The base 100 may be plate-shaped and may be fixed to the floor (FIGS. 4 and 5).

All of the above-described configurations can be installed on the base 100.

Specifically, for example, a seating portion 200 and a foundation frame FF may be installed on the base 100.

One or more fifth guide parts GR5 may be installed side by side on the base 100. The fifth guide part GR5 may be a guide rail.

The fifth guide part GR5 may extend in a second direction (eg, front-to-back direction). The fifth guide unit GR5 may guide the seating unit 200 to be withdrawn and retracted in the second direction.

[Seat part]

The seating portion 200 may be installed on the base 100 to be retractable and withdrawn in the second direction. Specifically, for example, the seating portion 200 may be slidably coupled to the fifth guide portion GR5 installed on the base 100 to extend in the second direction (FIGS. 5 and 6).

A plurality of seating units 200 may be provided (200a, 200b). The seating portions 200a and 200b may include a receiving groove 210. Additionally, at least a portion of the seating portion 200a may further include a heater 220 (FIG. 6).

A plurality of receiving grooves 210 (three in the drawing) may be formed in each seating portion 200. The cartridge 20 may be seated in the receiving groove 210.

The heater 220 may be provided on the inner bottom surface of the receiving groove 210 (FIG. 6). The heater 220 may be provided only on the bottom surface of the receiving groove 210 in which the cartridge 20, where a step requiring heating is performed among the nucleic acid extraction steps, is seated. Accordingly, as shown in FIG. 6, the receiving groove 210 of the seating portion 220a on one (e.g., right) of the two seating portions 200a and 200b disposed on both sides of the first direction (e.g., left and right direction) The heater 220 may be provided only on the bottom surface.

The heater 220 may be provided with a temperature sensor (not shown) in each receiving groove 210 in which the heater 220 is provided, and may be individually installed in each receiving groove 210 in which the heater 220 is provided. Temperature can be controlled.

Meanwhile, to extract nucleic acids, for example, six steps may be performed: a first lysis step, a second lysis step, a first washing step, a second washing step, a third washing step, and an elution step. As will be described later, the same nucleic acid extraction step can be performed in all wells W provided in the same cartridge 20, so 6 cartridges 20 may be required to perform 6 steps. Accordingly, as shown in the drawing, a total of six receiving grooves 210 may be formed in the seating portions 220a and 220b.

Among the six steps, steps requiring heating may be the first lysis step, the second lysis step, and the illusion step. The three cartridges 20 in which three steps requiring heating are performed include the right seating portion 220a equipped with a heater 22 among the two seating portions 200a and 200b disposed on both sides of the first direction described above. It can be collected and placed in. Accordingly, energy efficiency can be improved because heat can be transferred during heating.

[cartridge]

Figure 9 is a perspective view of a cartridge having multiple (6 x 4) wells according to one embodiment of the present invention.

With further reference to Figure 9, the cartridge 20 may be provided with one or more wells (W). A hollow with an open top may be formed in the well W. For example, a sample, magnetic bead, lysis buffer, wash buffer, elution buffer, etc. can be accommodated in the hollow.

A nucleic acid extraction step may be performed in each well (W). For example, the same nucleic acid extraction step may be performed in all wells W provided in the same cartridge 20.

[Basic frame]

The foundation frame (FF) may be fixedly installed on the base 100. The basic frame FF may be, for example, shaped like a table with a flat top (FIGS. 4 and 5).

One or more third guide parts GR3 may be installed side by side in the basic frame FF. The third guide part GR3 may be a guide rail.

The third guide part GR3 may extend in the first direction. The third guide unit GR3 may guide the main frame 300 to move in the first direction.

The basic frame FF may have a height higher than the height of the cartridge 20 seated on the seating portion 200 (FIG. 6). In this regard, it will be described later.

[mainframe]

The main frame 300 may include a bottom frame 310 and a top and bottom extension frame 320 (FIGS. 4 and 5).

The bottom frame 310 may be connected to the third driving unit 900 and may be slidably coupled to the third guide unit GR3. The floor frame 310 can move in the first direction by the third driving unit 900. This will be described later.

A support frame (SF) coupled to the cover 1000 and the fourth driving unit 1100 may be coupled to one side (eg, the front right side) of the bottom frame 310 ( FIGS. 4 and 5 ).

The upper and lower extension frames 320 may be combined with the bottom frame 310 and may extend upward and downward. A magnetic rod holder 500, a cap holder 600, a first driving part 700, and a second driving part 800 may be installed in the vertical extension frame 320.

One or more second guide parts GR2 may be installed side by side in the upper and lower extension frames 320. The second guide part GR2 may extend in the vertical direction. The second guide part GR2 may be a guide rail.

The second guide unit GR2 may guide the cap holder 600 and/or the first driving unit 700 to move in the vertical direction.

Meanwhile, the main frame 300 can be installed on the basic frame (FF) having a height higher than the height of the cartridge 20 seated on the seating unit 200, so the height of the main frame 300 is higher than the seating unit ( It may be higher than the height of the cartridge 20 seated in 200).

Accordingly, even if the vertical length (height) of the bottom frame 310 and the upper and lower extension frames 320 is reduced, the magnetic holder 500 and the cap holder 600 can move up and down on the upper part of the cartridge 20. there is. Accordingly, the size (length and height in the vertical direction) and weight of the main frame 300 can be reduced. Accordingly, energy efficiency is improved, movement control of the main frame 300 is facilitated, vibration of the main frame 300 itself is reduced, durability of the main frame 300 is improved, and a third guide combined with the main frame 300 is installed. Wear or deformation of the unit GR3 and the third driving unit 900 may be reduced. In addition, even if the support frame (SF) coupled with the cover 1000 and the fourth driving part 1100 is coupled to the main frame 300, the pressure applied to the third guide part GR3 and the third driving part 900 This increase can be prevented.

In addition, since the height of the main frame 300 is higher than the height of the cartridge 20 seated on the seating unit 200, the cover 1000 and the fourth driving unit 1100 coupled to the main frame 300 are connected to the main frame ( It may be placed at a low height below the height of the floor frame 310 of 300). Accordingly, the bottom frame 310 of the main frame 300 may be forced downward by the load of the cover 1000 and the fourth driving unit 1100. Therefore, when the main frame 300 moves in the first direction or the holders 500 and 600 installed on the main frame 300 move up and down, the vibration of the main frame 300 is reduced, improving stability, durability and nucleic acid extraction efficiency. This can be improved.

[Magnetic rod, magnetic rod holder]

10 and 11 are perspective views showing a magnetic rod holder combined with a magnetic rod according to an embodiment of the present invention.

10 and 11, the magnetic rod 400 may be coupled to the magnetic rod holder 500.

The magnetic rod 400 may enter the interior of the cap C coupled to the cap holder 600, which will be described later, or may be withdrawn from the interior of the cap C. At this time, in order for the magnetic rod 400 to enter the inside of the cap C, the magnetic rod 400 may penetrate the through hole 610 of the cap holder 600.

When the magnetic rod 400 enters the inside of the cap (C), the magnetic bead contained in the well (W) may stick to the cap (C), and when the magnetic rod 400 retreats from the inside of the cap (C), the cap The magnetic bead stuck to (C) can be separated from the cap (C). Accordingly, the magnetic rod 400 can be used to transfer the magnetic beads accommodated in the well (W) to another well (W) or to another cartridge (20). In the present invention, the nucleic acid extraction step can be performed sequentially by moving the magnetic beads to the well (W) of another cartridge using the magnetic rod 400.

The magnetic rod holder 500 may be combined with one or more magnetic rods 400. The magnetic rod holder 500 may be placed on the cap holder 600 (FIGS. 4 and 5).

The magnetic rod holder 500 can move up and down by the first driving unit 700. Additionally, the magnetic rod holder 500 can be moved up and down by the second driving unit 800. Additionally, the magnetic rod holder 500 can move in the first direction by the third driving unit 900. The driving unit will be described later.

[Cap holder, fixing device]

12 to 14 are perspective views, plan views, and front views of a cap holder equipped with a fixing device according to an embodiment of the present invention, showing the insertion hole of the cap holder in an open state. Figures 15 to 17 are a perspective view, plan view, and front view of a cap holder equipped with a fixing device according to an embodiment of the present invention, showing the insertion hole of the cap holder in a closed state. Figure 18 is an exploded perspective view of Figure 12. Figure 19 is a cross-sectional view of the rotating part of the fixture.

With further reference to FIGS. 12 to 18, the cap holder 600 is disposed below the magnetic rod holder 500 (FIGS. 4 and 5) and may be coupled with one or more caps C (FIG. 8 ). For example, the cap holder 600 may be combined with a strip 40 having one or more caps C. Here, the cap C and the strip 40 will be described later.

The cap holder 600 can move up and down by the second driving unit 800. Additionally, the cap holder 600 can move in the first direction by the third driving unit 900. The driving unit will be described later.

The cap holder 600 may include a through hole 610 and an insertion groove 620 (FIGS. 12 to 18).

The through hole 610 may be a hollow hole penetrating upward and downward and may be formed of one or more. The through hole 610 may communicate with the interior of the cap C coupled to the cap holder 600.

The magnetic rod 400 may enter or retreat from the interior of the cap C coupled to the cap holder 600 through the through hole 610 . Specifically, for example, the magnetic rod 400 may enter or be withdrawn from the inside of the cap C of the strip 40 inserted into the cap holder 600 through the through hole 610.

The insertion groove 620 may be formed in a second direction, for example, and may be hollow with one end (eg, front end) in the second direction open. The insertion groove 620 may communicate with the through hole 610 and at least a portion of the insertion groove 620 may overlap the through hole 610.

Additionally, one or more insertion grooves 620 may be formed and may be formed side by side in the first direction. A strip 40 may be inserted into each insertion groove 620.

The fixing device (L) may be installed at the end of the cap holder 600 where the insertion groove 620 is formed. Specifically, for example, the fixing device L may be installed on the upper surface of the front end of the cap holder 600 where the insertion groove 620 is formed.

The fixing device (L) can close the insertion groove 620 and fix the strip 40 inserted into the insertion groove 620 (FIGS. 8, 15, and 17). On the other hand, when the insertion groove 620 is opened by unfastening the fixing device (L), the strip 40 inserted into the insertion groove 620 can be removed and the strip 40 can be inserted into the insertion groove 620. there is.

Accordingly, the strip 40 can be stably fixed to the cap holder 600. Since vibration is generated by the driving units 700, 800, 900, and 1100 in the nucleic acid extraction device, it is difficult to maintain the strip 40 completely inserted into the insertion groove 620. If the strip 40 is not fully inserted into the insertion groove 620, the nucleic acid extraction device may be damaged and nucleic acid extraction may not be performed normally. In the present invention, by providing a fixing device (L) at the end of the cap holder 600, the strip 40 can be stably maintained fully inserted into the insertion groove 620 even if vibration occurs, thereby damaging the nucleic acid extraction device. This can be prevented and nucleic acid extraction efficiency can be improved.

Specifically, for example, the fixing device L may include a rotation support portion L1 fixed to an end of the cap holder 600 and a rotation portion L2 rotatably coupled to the rotation support portion L1. The insertion groove 620 may be opened and closed according to the rotation of the rotating part L2. For example, the rotation support portion L1 includes a rotation shaft L1a extending in the first direction and penetrating the rotation portion L2, and a coupling portion L1b that secures both ends of the rotation shaft L1a to the ends of the cap holder 600. L1c) (FIG. 18).

Accordingly, the fixing device L can be easily manufactured at low cost with a simple configuration and can be easily used.

Referring further to FIG. 19, a first magnet (L3A) may be installed in the rotating part (L2) (FIGS. 12, 14, 18, and 19). When the insertion groove 620 is closed as the rotary part L2 rotates (FIG. 15), the first magnet L3A may be attached to the end of the cap holder 600 (eg, the front of the cap holder).

Accordingly, the strip 40 can be stably fixed to the cap holder 600 easily and at low cost with a simple configuration. Additionally, when the first magnet L3A is attached to the end of the cap holder 600, vibration or sound may be generated and the rotating part L2 may be more strongly bound to the cap holder 600. It is possible to easily check whether the strip 40 is completely inserted into the insertion groove 620 through such vibration, sound, or change in binding force. Accordingly, it is possible to prevent damage to the nucleic acid extraction device due to incomplete insertion of the strip 40 into the cap holder 600.

Additionally, a second magnet (L3B) may be installed in the rotating part (L2) (FIGS. 15, 16, and 19). When the insertion groove 620 opens as the rotary part L2 rotates (FIG. 12), the second magnet L3B may be attached to the end of the cap holder 600 (eg, the front upper surface of the cap holder).

Accordingly, since the insertion groove 620 is stably maintained in an open state, the strip 40 can be easily inserted even if vibration occurs when inserting the strip 40 into the insertion groove 620.

[strip]

Figure 20 is a perspective view of a strip according to an embodiment of the present invention. Figure 21 is a diagram showing a state in which the strip of Figure 20 is inserted into the cap holder, the cap of the strip is inserted into the well of the cartridge, and the magnetic rod coupled to the magnetic rod holder is inserted into the cap.

20 and 21, the strip 40 may include a cap C and a connection portion 42.

One or more caps C (eg, 6 The cap (C) may extend up and down. A hollow top may be formed in the cap (C).

A magnetic rod 400 may be inserted into the cap (C). The cap C can pass the magnetic flux of the magnetic rod 400 entered inside and may be made of a non-magnetized material.

The cap C may enter or be withdrawn from the inside of the well W of the cartridge 20 inserted into the receiving groove 210 (FIG. 21).

The cap C may repeatedly enter and withdraw from the interior of the well W. Accordingly, the cap (C) may be a stirring rod.

Meanwhile, unlike the drawing, each cap C may be directly coupled to the cap holder 600 without the strip 40 as an intermediary. Alternatively, the cap C may be integrally coupled with the cap holder 600.

The connection portion 42 may extend in a direction (eg, first direction and second direction) that intersects the direction in which the cap C extends (up and down direction). The connection portion 42 can connect one or more caps (C).

The connection portion 42 may constitute the upper end of the strip 40. The connection portion 42 may be inserted into the insertion groove 620 of the cap holder 600.

After the connection part 42 is completely inserted into the insertion groove 620 of the cap holder 600, the rotating part L2 of the fixing device L can close the insertion groove 620, and the first part of the rotating part L2 can close the insertion groove 620. A magnet L3A may be attached to the end of the cap holder 600.

Hereinafter, the second drive unit will be examined before the first drive unit, and the remaining components will be examined in order.

[Second District East]

Figures 22 and 23 are perspective views showing main components related to the second driving unit according to an embodiment of the present invention. Figures 24 and 25 are exploded perspective views of Figures 22 and 23.

22 to 25, the second driving unit 800 may be installed on the main frame 300. Specifically, for example, the second driving unit 800 may be installed on the vertical extension frame 320.

The second driving unit 800 may be connected to the cap holder 600 and the first driving unit 700 and may move the cap holder 600 and the first driving unit 700 up and down together.

As the second driving part 800 moves the cap holder 600 up and down, the second driving part 800 moves the cap C coupled to the cap holder 600 into the receiving groove 210 of the seating part 200. It can be entered into the inside of the well W of the seated cartridge 20 or withdrawn from the inside of the well W.

Specifically, for example, the second driving unit 800 includes a second motor 810, a second driving pulley 820a, a second driven pulley 820b, a second belt 830, and a second ball screw bolt 840. ), a second ball screw nut 850, and a second coupler (860a, 860b, 860c, 860d, 860e).

The second motor 810 may be installed on the floor frame 310 or the vertical extension frame 320. The rotation axis of the second motor 810 may be coupled to the second driving pulley 820a.

The second driving pulley 820a and the second driven pulley 820b may be installed on the vertical extension frame 320 and may be connected to each other through the second belt 830.

The second belt 830 may have irregularities formed on one surface facing the pulleys 820a and 820b.

When the second driving pulley (820a) rotates by the second motor (810), the second driven pulley (820b) can rotate by the second belt (830).

The second ball screw bolt 840 (FIG. 24) is coupled with the second driven pulley 820b and can rotate together.

The second ball screw nut 850 may be supported by being coupled to the second ball screw bolt 840 and may be coupled to the second coupler 860.

When the second driven pulley 820b rotates and the second ball screw bolt 840 rotates, the second ball screw nut 850 can be raised and lowered.

The second coupler 860 may be combined with the second ball screw nut 850, the cap holder 600, and the first motor 710.

Specifically, for example, the second coupler 860 includes a first coupling member 860a coupled with the second ball screw nut 850, a second coupling member coupled with the first coupling member 860a and the cap holder 600. A coupling member 860b, a third coupling member 860c coupled with the second coupling member 860b, a fourth coupling member 860d coupled with the third coupling member 860c, and a fourth coupling member 860d. And it may include a fifth coupling member 860e coupled to the first motor 710. The first driven pulley 720b of the first driving unit 700, which will be described later, may be coupled to the fourth coupling member 860d.

Accordingly, the second coupler 860 can move the cap holder 600 and the first motor 710 up and down. When the first motor 710 moves up and down, the entire first driving unit 700 can move up and down. In this regard, it will be described later.

Meanwhile, the first coupling member 860a of the second coupler 860 may be slidably coupled to the two second guide parts GR2. For example, the first coupling member 860a may be coupled to two second guide coupling parts M2 that are slidably coupled to the two second guide parts GR2a and GR2b.

Accordingly, when the second coupler 860 is raised and lowered, it is guided by two second guide parts GR2 arranged side by side at a predetermined interval, so that the cap holder 600 and the first driving part 710 move to one side. You can go up and down stably without tilting. In particular, since the cap holder 600 can quickly and stably vibrate up and down, stirring work can be performed effectively and stably. Additionally, even if the size or width of the cap holder 600 is large, the cap holder 600 can be stably raised and lowered without tilting to one side.

Meanwhile, a second sensor (S2) for setting the reference height of the cap holder 600 and the second coupler 860 may be disposed on the main frame 300, the cap holder 600, or the second driving unit 800. there is. The second sensor S2 may be connected to the control unit 1200.

Specifically, for example, as shown in Figure 24, the second protrusion (P2) may be formed on the first coupling member (860a), and the second sensor (S2) that detects the second protrusion (P2) may be connected to the vertical extension frame ( 320) may be placed at a predetermined height (eg, a height corresponding to the highest height of the cap holder). When the first coupling member 860a moves up and down by the second motor 810 and the second protrusion P2 is positioned at the predetermined height, the second sensor S2 detects the second protrusion P2. Thus, the sensed information can be transmitted to the control unit 1200, and the control unit 1200 can set a reference position (reference height) for controlling the second motor 810.

[First District East]

Figures 26 and 27 are perspective views showing main components related to the first driving unit according to an embodiment of the present invention. Figures 28 and 29 are exploded perspective views of Figures 26 and 27.

Referring further to FIGS. 26 to 29 , the first driving unit 700 may be installed in the second coupler 860 of the second driving unit 800. That is, the first driving unit 700 can be installed to move up and down on the vertical extension frame 320 through the second driving unit 800.

The first driving unit 700 may be connected to the magnetic rod holder 500 and may move the magnetic rod holder 500 up and down.

Accordingly, the first driving unit 700 causes the magnetic rod 400 coupled to the magnetic rod holder 500 to enter the interior of the cap C coupled to the cap holder 600 or to retreat from the interior of the cap C. You can do it.

Specifically, for example, the first driving unit 700 includes a first motor 710, a first driving pulley 720a, a first driven pulley 720b, a first belt 730, and a first ball screw bolt 740. ), a first ball screw nut 750, and a first coupler (760a, 760b).

The first motor 710 may be installed in the fifth coupling member 860d of the second coupler 860 of the second driving unit 800. Accordingly, the height of the first motor 710 may be changed by the second driving unit 800.

The rotation axis of the first motor 710 may be coupled to the first driving pulley 720a.

The first driving pulley (720a) and the first driven pulley (720b) may be installed on the fourth/fifth coupling members (860d, 860e) of the second coupler (860) of the second driving part (800), and the first They may be connected to each other through a belt 730.

The first belt 730 may have irregularities formed on one surface facing the pulleys 720a and 720b.

When the first driving pulley 720a rotates by the first motor 710, the first driven pulley 720b can rotate by the first belt 730.

The first ball screw bolt 740 (FIG. 28) is coupled with the first driven pulley 720b and can rotate together.

The first ball screw nut 750 may be supported by being coupled to the first ball screw bolt 740 and may be coupled to the first coupler 760.

When the first driven pulley 720b rotates and the first ball screw bolt 740 rotates, the first ball screw nut 750 can be raised and lowered.

The first coupler 760 may be combined with the first ball screw nut 750 and the magnetic rod holder 500.

Specifically, for example, the first coupler 760 is a first coupling member 760a coupled to the first ball screw nut 750, a first coupling member 760a, and a first coupling member 760a coupled to the magnetic rod holder 500. It may include two coupling members (760b).

Accordingly, the first coupler 760 can move the magnetic rod holder 500 up and down.

Meanwhile, the first ball screw bolt 740, the first ball screw nut 740, and the first coupler 760 are directly or indirectly coupled to the first motor 710, so that the first motor 710 is connected to the second driving unit. When moved up and down by 800, the entire first driving unit 700 can move up and down.

Additionally, when the first motor 710 moves up and down by the second driving part 800, the magnetic rod holder 500 coupled to the first driving part 700 can also move up and down. Therefore, the height of the magnetic rod holder 500 is based on the first height determined by the second driving part 800 (e.g., the height of the cap holder, the second coupler, or the first motor) and the first driving part ( It can be calculated by adding up the second height (relative height to the first height) determined by 700).

The first coupler 760 and the second coupler 860 may be provided with a first guide part GR1 and a first guide coupling part M1 that is slidably coupled to the first guide part GR1. In the drawing, the first guide part GR1 is installed on the second coupling member 760b of the first coupler 760, and the first guide coupling part M1 is installed on the second coupler 860.

Meanwhile, a first sensor (S1) for setting the reference height of the magnetic rod holder 500 may be disposed in the magnetic rod holder 500, the first driving unit 700, or the second driving unit 800. The first sensor S1 may be connected to the control unit 1200.

Specifically, for example, as shown in FIGS. 28 and 29, the first protrusion P1 may be formed on the first coupling member 760a of the first coupler 760, and a first protrusion P1 may be detected. One sensor S1 may be placed at a predetermined height (eg, a height corresponding to the highest height of the magnetic holder) of the second coupling member 840b of the second coupler 860. The first coupling member 760a of the first coupler 760 moves up and down by the first motor 710 so that the first protrusion P1 is relative to the second coupling member 860b of the second coupler 860. When located at the predetermined height, the first sensor (S1) can detect the first protrusion (P1) and transmit the detected information to the control unit 1200, and the control unit 1200 controls the first motor 710. You can set the reference position (reference height) for this.

As described above, the second driving unit 800 can move the cap holder 600 and the first driving unit 700 up and down together.

Accordingly, when the second driving part 800 moves the cap holder 600 up and down, the magnetic rod holder 500 coupled to the first driving part 700 can also move up and down together. Accordingly, when the height of the cap holder 600 changes, the height of the magnetic rod holder 500 may also change equally. Accordingly, the cap holder 600 and the magnetic rod holder 500 can be controlled more simply and stably than when they move up and down independently of each other, and malfunctions such as misjudging the positions (heights) of the holders 500 and 600 can be prevented. It can prevent damage to the nucleic acid extraction device.

Specifically, the cap holder 600 rises together with the magnetic rod holder 500, so when the cap holder 600 is raised, the cap holder 600 or the cap (C) moves with the magnetic rod holder 500 or the magnetic rod 400. ) There is no need to control it to avoid colliding with it. In addition, since the cap holder 600 goes up and down together with the magnetic rod holder 500, the magnetic rod holder 500 always moves up and down the cap holder 600 by a predetermined distance (height). It can be located at the top. Accordingly, since the magnetic rod holder 500 can stably descend by the predetermined distance (height), there is no need to consider the position (height) of the cap holder 600 when lowering the magnetic rod holder 500.

On the other hand, in the related art, in order to prevent damage or malfunction of the nucleic acid extraction device due to collision between the cap holder 600 or cap C and the magnetic rod holder 500 or magnetic rod 400, the holders 500 and 600 The current position (height) was determined/detected and the holders (500, 600) were moved based on this. Accordingly, control of the nucleic acid extraction device becomes complicated, unexpected errors may occur, and if the current position (height) of the holders 500, 600 is incorrectly determined due to a malfunction of the sensor, etc., there is a problem of damage to the nucleic acid extraction device.

[3rd District East]

Figures 30 and 31 are perspective views showing main components related to the third driving unit according to an embodiment of the present invention. Figures 32 and 33 are exploded perspective views of Figures 30 and 31.

Referring to FIGS. 30 to 33 , the third driving unit 900 may be installed on the base 100. Specifically, for example, the third driving unit 900 may be installed on the basic frame FF installed on the base 100.

The third driving unit 900 can move the main frame 300 in the first direction. Accordingly, the third driving unit 900 can move the magnetic rod holder 500 and the cap holder 600 installed on the main frame 300 in the first direction. Accordingly, the third driving unit 900 connects the cap C coupled to the cap holder 600 and the magnetic rod 400 coupled to the magnetic rod holder 500 to another well W in the first direction, for example, another well. It can be located at the top of the well (W) of the cartridge 40.

However, it is not limited to this configuration. For example, the third driving unit 900 may move the seating unit 200 in the first direction, unlike the drawing.

Specifically, for example, the third driving unit 900 includes a third motor 910, a third driving pulley (920a), a third driven pulley (920b), a third belt 930, and a third ball screw bolt (940). ) and a third ball screw nut 950.

The third motor 910 may be installed on the basic frame (FF). The rotation axis of the third motor 910 may be coupled to the third driving pulley 920a.

The third driving pulley 920a and the third driven pulley 920b may be installed on the basic frame FF and may be connected to each other through the third belt 930.

The third belt 930 may have irregularities formed on one surface facing the pulleys 920a and 920b.

When the third driving pulley 920a is rotated by the third motor 910, the third driven pulley 920b can be rotated by the third belt 930.

The third ball screw bolt 940 (FIGS. 30 to 33) may be coupled with the third driven pulley 820b and rotate together.

The third ball screw nut 950 may be coupled to the third ball screw bolt 940 and the bottom frame 310 of the main frame 300.

When the third driven pulley (920b) rotates and the third ball screw bolt (940) rotates, the third ball screw nut (950) and the main frame (300) coupled with the third ball screw nut (950) rotate in the first direction. You can move to .

However, it is not limited to this configuration. That is, for example, unlike the drawings, the third driving unit 900 may move the seating unit 100 in the first direction.

Meanwhile, the main frame 300 may be slidably coupled to one or more third guide parts GR3 installed on the basic frame FF. For example, the bottom frame 310 of the main frame 300 may be coupled to two third guide coupling parts M3 that are slidably coupled to the two third guide parts GR3. Accordingly, the mainframe 300 can move stably.

A third sensor (S3) may be placed on the base 100, basic frame (FF), main frame 300, or third driving unit 900 to set the reference position in the first direction of the main frame 300. there is. The third sensor S3 may be connected to the control unit 1200.

Specifically, for example, as shown in FIGS. 31 and 33, a third protrusion (P3) may be formed on the bottom frame 310 of the main frame 300, and a third sensor ( S3) may be placed at a predetermined position in the basic frame FF. When the floor frame 310 moves in the first direction by the third motor 910 and the third protrusion P3 is located at the predetermined position, the third sensor S3 detects the third protrusion P3. Thus, the sensed information can be transmitted to the control unit 1200, and the control unit 1200 can set a reference position for controlling the third motor 910.

[Cover, support frame, 4th drive unit]

Figures 34 and 35 are perspective views showing the main frame, support frame, cover, and fourth driving unit according to an embodiment of the present invention. Figures 36 and 37 are exploded perspective views of Figures 34 and 35.

34 to 37, a support frame (SF) may be coupled to one side (e.g., the front right side) of the bottom frame 310 of the main frame 300, and a cover 1000 may be attached to the support frame (SF). and a fourth driving unit 1100 may be installed. Accordingly, the cover 1000 and the fourth driving unit 1100 can be installed on the main frame 300 through the support frame (SF).

Accordingly, when the third driving unit 900 moves the main frame 300 in the first direction, the magnetic rod holder 500, the cap holder 600, the first driving unit 700, and the magnetic rod holder 500 installed on the vertical extension frame 320. The second driving part 800, the cover 1000 installed on the support frame (SF), and the fourth driving part 1100 can be moved together in the first direction. That is, the cover 1000 and the fourth driving unit 1100 may be installed to be movable in the first direction along the cap holder 600.

Accordingly, when the position of the cap holder 600 in the first direction changes, the positions of the cover 1000 and the fourth driving unit 1100 in the first direction may also change in the same way. Accordingly, the cap holder 600, cover 1000, and fourth driving unit 1100 can be controlled more simply and stably than when they move in the first direction independently of each other. On the other hand, when the cap holder 600, the cover 1000, and the fourth driving unit 1100 move in the first direction independently of each other, the cover 600 is based on the current position of the cap holder 600 before moving the cover 1000. The moving distance of (1000) must be calculated.

In addition, due to a malfunction, such as misjudging the position (height) of the cap holder 600, the cover 1000 collides with the cap holder 600, etc., damaging the nucleic acid extraction device, or the cover 1000 moves to the wrong position and the well ( It is possible to prevent foreign substances from entering W).

However, it is not limited to this configuration. That is, the cover 1000 and the fourth driving unit 1100 may be installed independently of the main frame 300, and the cover 1000 may move in the first direction independently of the cap holder 600, etc.

The support frame (SF) may be spaced apart from the upper and lower extension frames 320. That is, as shown in the drawing, the support frame (SF) and the upper and lower extension frames 320 do not contact each other, but may be connected through the floor frame 310.

Accordingly, vibration transmitted between the upper and lower extension frames 320 and the support frame (SF) can be reduced. Accordingly, the holders 500 and 600 installed on the upper and lower extension frames 320, the first and second driving parts 700 and 800, and the cover 1000 and the fourth driving part 1100 installed on the support frame (SF) are transmitted. By reducing vibration, the stability, durability, and nucleic acid extraction efficiency of the nucleic acid extraction device can be improved. Meanwhile, at least a portion of the support frame SF is disposed on one side (e.g., on the right side) of the cap holder 600 in the first direction, and is disposed overlapping with the cap holder 600 in the second direction and is attached to the seating portion 200. It can be placed higher than the seated cartridge 20 (FIGS. 6, 34, 38 to 55).

Cover 1000 may be installed on the main frame 300. For example, the cover 1000 may be installed on a support frame (SF) coupled to one side of the bottom frame 310 of the main frame 300. The cover 1000 may have a plate shape.

The cover 1000 may enter or retreat from the space between the cartridge 20 seated on the seating portion 200 and the cap C coupled to the cap holder 600 by the fourth driving unit 1200. For example, the cover 1000 may be placed higher than the cartridge 20 seated on the seating portion 200, and one side (e.g., right side) of the cover 1000 in the first direction may be used as a ball screw to be described later. It may be coupled to the nut 1150, and the other side (eg, left side) in the first direction may enter or retreat from the space in the first direction (FIGS. 6, 34, and 38 to 55).

Specifically, the cover 1000 is used to perform the next step of the current step among the plurality of steps for extracting nucleic acids. After the cap C coupled to the cap holder 600 is retracted from the inside of the well W, When moving to the top of the well (W), it may enter the space between the cartridge 20 seated on the seating portion 200 and the cap C coupled to the cap holder 600 (FIGS. 46 to 49) .

In addition, the cover 1000 is used to protect the cartridge 20 and the cap holder 600 mounted on the seating portion 200 when the cap C coupled to the cap holder 600 must enter the inside of the well W. ) can be retracted from the space between the caps (C) coupled to (FIGS. 50 to 55).

Accordingly, materials such as magnetic beads, cleaning fluid (eg, ethanol), and samples that fall from the cap (C) can be prevented from flowing into the well (W). In particular, even if the movement distance of the cap C in the first direction is long or vibration/shock occurs during movement, foreign matter can be prevented from entering the well W located at the bottom of the movement path. Accordingly, nucleic acid extraction efficiency can be improved.

In addition, when extracting a large amount of nucleic acid at once, a large amount of magnetic beads must be transferred to another cartridge 20 or another well (W) at once to perform the next step of nucleic acid extraction. At this time, some of the large amount of magnetic beads attached by magnetic force to the cap (C) into which the magnetic rod 400 is inserted can be easily separated, and substances such as cleaning fluid existing in the space between the large amount of magnetic beads are attached to the cap (C). C) may fall. In this way, even if a large amount of magnetic beads are attached to the cap (C) and moved all at once, the cover (1000) can prevent foreign substances from entering the well (W). Accordingly, with a simple structure, a large amount of nucleic acid can be extracted correctly and effectively at low cost and easily.

A groove 1010 may be formed on the upper surface of the cover 1000 to contain materials that fall from the cap C coupled to the cap holder 600.

Accordingly, the material falling from the cap C can be easily collected at low cost with a simple configuration. Accordingly, since there is no need for a separate configuration to collect materials falling from the cap C, manufacturing and maintenance can be facilitated at low cost.

As shown in the drawing, the cover 1000 may enter or retreat from the space between the cartridge 20 and the cap C in the first direction by the fourth driving unit 1100.

Accordingly, the cover 1000 and the fourth driving unit 1100 can be placed and operated close to the main frame 300, so vibration can be reduced and pressure applied to the main frame 300, etc. can be reduced. Accordingly, the durability, stability, and nucleic acid extraction efficiency of the nucleic acid extraction device can be improved.

Additionally, the cover 1000 can be included in the nucleic acid extraction device by simply providing an additional space the size of the cover 1000 in the first direction. For example, if the cover 1000 is designed to enter or retreat into the space between the cartridge 20 and the cap C in the first direction, the cover 1000 can operate in an existing nucleic acid extraction device as long as the additional space is secured. can do. Accordingly, the nucleic acid extraction device can be miniaturized and lightweight, and costs can be reduced.

The fourth driving unit 1100 may be installed on the main frame 300. Specifically, for example, the fourth driving unit 1100 may be installed on the support frame (SF) coupled to one side of the bottom frame 310 of the main frame 300.

The fourth driving unit 1100 may move the cover 1000 in the first direction. Accordingly, the fourth driving unit 1100 moves the cover 1000 into the space between the cartridge 20 seated on the seating unit 200 and the cap C coupled to the cap holder 600 or from the space. It can be evacuated.

However, it is not limited to this configuration. For example, unlike the drawing, the fourth driving unit 1100 may move the cover 1000 in the second direction.

Specifically, for example, the fourth driving unit 1100 includes a fourth motor 1110, a fourth driving pulley (1120a), a fourth driven pulley (1120b), a fourth belt (1130), and a fourth ball screw bolt (1140). ), a fourth ball screw nut 1150, and a fourth coupler 1160.

The fourth motor 1110 may be installed on the support frame (SF). The rotation axis of the fourth motor 1110 may be coupled to the fourth driving pulley 1120a.

The fourth driving pulley 1120a and the fourth driven pulley 1120b may be installed on the support frame SF and may be connected to each other through the fourth belt 1130.

The fourth belt 1130 may have irregularities formed on one surface facing the pulleys 1120a and 1120b.

When the fourth driving pulley (1120a) rotates by the fourth motor (110), the fourth driven pulley (1120b) can rotate by the fourth belt (1130).

The fourth ball screw bolt 1140 is coupled with the fourth driven pulley 1120b and can rotate together. The fourth ball screw bolt 1140 is disposed on one side (e.g., right) of the cap holder 600 in a first direction (e.g., left and right direction), and moves in a second direction (e.g., front and rear) that intersects the up and down direction and the first direction. direction) and may be arranged to overlap with the cap holder 600, and may be placed higher than the cartridge 20 seated on the seating portion 200. The fourth ball screw bolt 1140 may extend in the first direction (FIGS. 6, 34, and 38 to 55).

The fourth ball screw nut 1150 may be supported by being coupled to the fourth ball screw bolt 1140 and may be coupled to the fourth coupler 1160. The fourth ball screw nut 1150 may be placed higher than the cartridge 20 seated on the seating portion 200 (FIGS. 6, 34, 38 to 55).

The fourth coupler 1160 may be combined with the fourth ball screw nut 1150 and the cover 1000.

Accordingly, when the fourth driven pulley (1120b) rotates and the fourth ball screw bolt (1140) rotates, the fourth ball screw nut (1150) and the cover (1000) combined with the fourth ball screw nut (1150) are connected to the first You can move in any direction.

Meanwhile, the fourth coupler 1160 may be slidably coupled to one or more fourth guide parts GR4 installed on the support frame SF. For example, the fourth coupler 1160 may be coupled to two fourth guide coupling parts M4 that are slidably coupled to the two fourth guide parts GR4. The fourth guide coupling portion M4 may extend in the first direction. Accordingly, the cover 1000 can move stably.

A fourth sensor S4 may be disposed on the support frame SF, the cover 1000, or the fourth driving unit 1100 to set the reference position of the cover 1000 in the first direction. The fourth sensor S4 may be connected to the control unit 1200.

Specifically, for example, as shown in FIGS. 36 and 37, the fourth protrusion P4 may be formed on the fourth coupler 1160 of the fourth driving unit 1100, and the fourth protrusion P4 may be detected. The sensor S4 may be placed at a predetermined position on the support frame SF. When the fourth coupler 1160 moves in the first direction by the fourth motor 1110 and the fourth protrusion (P4) is located at the predetermined position, the fourth sensor (S4) moves the fourth protrusion (P4). The detected information can be transmitted to the control unit 1200, and the control unit 1200 can set a reference position for controlling the fourth motor 1110.

[Control unit]

The control unit 1200 can control the first/second/third/fourth driving units 700, 800, 900, and 1100 and can control the temperature of the heater 220 of the seating unit 200.

In addition, the control unit 800 controls the first / Control information of the second/third/fourth driving units 700, 800, 900, and 1100 or control information of the temperature of the heater 220 may be stored.

The control unit 800 may control the first/second/third/fourth driving units 700, 800, 900, and 1100 or control the temperature of the heater 220 based on the control information.

[housing]

The housing 1300 can have the appearance of a nucleic acid extraction device, and can accommodate the first/second/third/fourth driving units (700, 800, 900, 1100), etc. inside. The third/fourth driving units (700, 800, 900, 1100), etc. can be protected.

[door]

The door 1400 can be formed in the housing 1300 and can be opened and closed. When the door 1400 is opened, the seating portion 200 can be pulled out and the cartridge 20 can be inserted.

[Display part]

The display unit 1500 may display the currently performed step among the plurality of steps.

[Input section]

The input unit 1600 may be a touch screen device provided together with the display unit 1500.

The input unit 1600 may receive control information of the first/second/third/fourth driving units 700, 800, 900, and 1100 or control information of the temperature of the heater 220 from the user.

[Specific Example]

Figures 38 to 55 are perspective and front views showing the process of moving magnetic beads using a nucleic acid extraction device according to an embodiment of the present invention.

Referring to FIGS. 38 and 39, both the cap holder 600 and the magnetic rod holder 500 are initially raised, and the cap C does not enter the well W of the cartridge 20 but is attached to the magnetic rod. (400) may not enter the inside of the cap (C). Additionally, the cover 1000 may not enter the space between the cartridge 20 and the cap C.

40 and 41, in order to perform one of the plurality of nucleic acid extraction steps, the cap holder 600 is lowered so that the cap C can enter the inside of the well W of the cartridge 20. there is. At this time, since the cap holder 600 is lowered, the magnetic rod holder 500 can also be lowered as described above.

Referring to FIGS. 42 and 43 , the magnetic rod holder 500 may descend so that the magnetic rod 400 may enter the inside of the cap (C). Accordingly, the purpose is to cause the magnetic beads accommodated in the well (W) to attach to the surface of the cap (C) by the magnetic force of the magnetic rod (400).

Referring to FIGS. 44 and 45 , the cap holder 600 rises so that the cap C can be retracted from the inside of the well W of the cartridge 20. As the cap holder 600 rises, the magnetic rod holder 500 can also rise together. However, the magnetic rod 400 may remain inside the cap (C). Accordingly, the magnetic bead can remain attached to the surface of the cap (C).

Referring to FIGS. 46 to 49, the cover 1000 can enter the space between the cartridge 20 and the cap C (FIGS. 46 and 47), and the cover 1000 has entered the space. The cap holder 600, etc. may move in the first direction (FIGS. 48 and 49). Accordingly, when moving the cap C in the first direction, foreign matter dropped from the surface of the cap C can be prevented from flowing into the well W of the cartridge 20 located below the movement path.

50 and 51, the cover 1000 may be retracted from the space between the cartridge 20 and the cap C.

Referring to FIGS. 52 and 53 , the cap holder 600 descends so that the cap C can enter the inside of the well W of the new cartridge 20. At this time, since the cap holder 600 descends, the magnetic rod holder 500 may also descend.

Referring to FIGS. 54 and 55 , the magnetic rod holder 500 may rise and the magnetic rod 400 may be retracted from the inside of the cap C. Accordingly, the magnetic beads that were attached to the surface of the cap (C) by magnetic force can fall off the surface of the cap (C) and react freely in the well (W) of the new cartridge (20).

As described above, the present invention has been described with reference to the illustrative drawings, but the present invention is not limited to the embodiments and drawings disclosed herein, and various modifications may be made by those skilled in the art within the scope of the technical idea of the present invention. It is obvious that transformation can occur. In addition, although the operational effects according to the configuration of the present invention were not explicitly described and explained while explaining the embodiments of the present invention above, it is natural that the predictable effects due to the configuration should also be recognized.

10: Nucleic acid extraction device
20: cartridge 40: strip
100: Base FF: Basic frame
200: seating portion 210: receiving groove
220: heater
300: main frame 310: floor frame
320: Top and bottom extension frame
400: magnetic rod 500: magnetic rod holder
600: Cap holder 610: Through hole
620: Insertion groove L: Fixing device
700: first driving unit 710: first motor
720: first pulley 730: first belt
740: 1st ball screw bolt 750: 1st ball screw nut
760: first coupler
800: second driving unit 810: second motor
820: second pulley 830: second belt
840: 2nd ball screw bolt 850: 2nd ball screw nut
860: Second coupler
900: Third driving unit 910: Third motor
920: Third pulley 930: Third belt
940: Third ball screw bolt 950: Third ball screw nut
1000: Cover
1100: 4th driving unit 1110: 4th motor
1120: 4th pulley 1130: 4th belt
1140: Fourth ball screw bolt 1150: Fourth ball screw nut
1160: Fourth coupler
1200: Control unit
1300: Housing 1400: Door
1500: display unit 1600: input unit

Claims (3)

A seating portion 200 on which a cartridge 20 having one or more wells (W) is seated;
One or more magnetic rods 400;
A magnetic rod holder 500 coupled to the magnetic rod 400 and installed to be movable up and down on the seating portion 200;
It is coupled to one or more caps (C), is disposed below the magnetic rod holder 500, and is installed to be movable up and down on the seating portion 200 to attach the cap (C) to the seating portion 200. A cap holder 600 that allows the cartridge 20 seated in the well W to enter or retreat from the well W;
a third driving unit 900 that moves the magnetic rod holder 500 and the cap holder 600 or the seating unit 200 in a first direction crossing the vertical direction;
In a state where the cap (C) is retracted from the inside of the well (W), it enters the space between the cartridge (20) seated on the seating portion (200) and the cap (C) coupled to the cap holder (600). A retractable cover (1000) and
It includes a fourth driving unit (1100) that allows the cover (1000) to enter or retreat from the space,
The cover 1000 and the fourth driving unit 1100 are installed to be movable in a first direction along the cap holder 600,
The cover 1000 is disposed higher than the cartridge 20 seated on the seating portion 200,
Nucleic acid extraction device.
In claim 1,
The fourth driving unit 1100 includes a fourth motor 1110 that causes the cover 1000 to enter or retreat from the space,
The fourth motor (1110) is installed to be movable in a first direction along the cap holder (600).
In claim 2,
The fourth driving unit 1100 is disposed on one side of the cap holder 600 in a first direction and overlaps the cap holder 600 in a vertical direction and a second direction intersecting the first direction, and is mounted on the fourth driving unit 1100. A nucleic acid extraction device disposed higher than the cartridge 20 seated in the unit 200.