KR102623312B1 - Ruvc 도메인이 존재하는 효소 - Google Patents
Ruvc 도메인이 존재하는 효소 Download PDFInfo
- Publication number
- KR102623312B1 KR102623312B1 KR1020217028874A KR20217028874A KR102623312B1 KR 102623312 B1 KR102623312 B1 KR 102623312B1 KR 1020217028874 A KR1020217028874 A KR 1020217028874A KR 20217028874 A KR20217028874 A KR 20217028874A KR 102623312 B1 KR102623312 B1 KR 102623312B1
- Authority
- KR
- South Korea
- Prior art keywords
- delete delete
- sequence
- seq
- endonuclease
- cases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020247000311A KR20240007322A (ko) | 2019-02-14 | 2020-02-14 | Ruvc 도메인이 존재하는 효소 |
Applications Claiming Priority (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962805868P | 2019-02-14 | 2019-02-14 | |
| US201962805878P | 2019-02-14 | 2019-02-14 | |
| US201962805899P | 2019-02-14 | 2019-02-14 | |
| US62/805,878 | 2019-02-14 | ||
| US62/805,868 | 2019-02-14 | ||
| US62/805,899 | 2019-02-14 | ||
| US201962874414P | 2019-07-15 | 2019-07-15 | |
| US62/874,414 | 2019-07-15 | ||
| PCT/US2020/018432 WO2020168291A1 (en) | 2019-02-14 | 2020-02-14 | Enzymes with ruvc domains |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020247000311A Division KR20240007322A (ko) | 2019-02-14 | 2020-02-14 | Ruvc 도메인이 존재하는 효소 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20210139254A KR20210139254A (ko) | 2021-11-22 |
| KR102623312B1 true KR102623312B1 (ko) | 2024-01-09 |
Family
ID=72045639
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020217028874A Active KR102623312B1 (ko) | 2019-02-14 | 2020-02-14 | Ruvc 도메인이 존재하는 효소 |
| KR1020247000311A Pending KR20240007322A (ko) | 2019-02-14 | 2020-02-14 | Ruvc 도메인이 존재하는 효소 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020247000311A Pending KR20240007322A (ko) | 2019-02-14 | 2020-02-14 | Ruvc 도메인이 존재하는 효소 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20240117330A1 (https=) |
| EP (1) | EP3924482A4 (https=) |
| JP (3) | JP2022520428A (https=) |
| KR (2) | KR102623312B1 (https=) |
| CN (2) | CN113728098A (https=) |
| AU (3) | AU2020223370B2 (https=) |
| CA (2) | CA3130135C (https=) |
| MX (2) | MX2021009886A (https=) |
| WO (1) | WO2020168291A1 (https=) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| US10913941B2 (en) | 2019-02-14 | 2021-02-09 | Metagenomi Ip Technologies, Llc | Enzymes with RuvC domains |
| AU2020223370B2 (en) * | 2019-02-14 | 2023-04-20 | Metagenomi Therapeutics, Inc. | Enzymes with RuvC domains |
| GB2594339B (en) * | 2020-02-14 | 2023-02-08 | Metagenomi Inc | Enzymes with RUVC domains |
| GB2617658B (en) | 2020-03-06 | 2024-04-17 | Metagenomi Inc | Class II, type V CRISPR systems |
| GB2608292B (en) * | 2020-03-31 | 2024-02-14 | Metagenomi Inc | Class II, type II CRISPR systems |
| EP4165177A4 (en) * | 2020-05-08 | 2024-08-28 | Metagenomi, Inc. | ENZYMES WITH RUVC DOMAINS |
| KR20230021657A (ko) * | 2020-05-08 | 2023-02-14 | 메타지노미, 인크. | Ruvc 도메인을 포함하는 효소 |
| CA3192224A1 (en) | 2020-09-11 | 2022-03-17 | Jyun-Liang LIN | Base editing enzymes |
| GB2700205B (en) * | 2020-09-11 | 2026-04-15 | Metagenomi Therapeutics Inc | Base editing enzymes |
| US20230383273A1 (en) * | 2020-10-21 | 2023-11-30 | Emendobio Inc. | Novel omni 56, 58, 65, 68, 71, 75, 78, and 84 crispr nucleases |
| WO2022098681A2 (en) * | 2020-11-03 | 2022-05-12 | Caspr Biotech Corporation | Novel class 2 crispr-cas rna-guided endonucleases |
| WO2022159742A1 (en) * | 2021-01-22 | 2022-07-28 | Metagenomi, Inc | Novel engineered and chimeric nucleases |
| BR112023014719A2 (pt) * | 2021-01-22 | 2023-12-05 | Metagenomi Inc | Novas nucleases quiméricas e manipuladas |
| BR112023018948A2 (pt) * | 2021-03-19 | 2023-10-17 | Metagenomi Inc | Edição multiplex com enzimas cas |
| WO2022232638A2 (en) * | 2021-04-30 | 2022-11-03 | Metagenomi, Inc. | Enzymes with ruvc domains |
| CA3225082A1 (en) * | 2021-08-27 | 2023-03-02 | Brian C. Thomas | Enzymes with ruvc domains |
| AU2022380842A1 (en) | 2021-11-05 | 2024-05-23 | Metagenomi Therapeutics, Inc. | Base editing enzymes |
| MX2024006257A (es) | 2021-11-24 | 2024-07-29 | Metagenomi Inc | Sistemas de endonucleasas. |
| WO2023122663A2 (en) * | 2021-12-23 | 2023-06-29 | Mammoth Biosciences, Inc. | Effector proteins and methods of use |
| WO2023194359A1 (en) * | 2022-04-04 | 2023-10-12 | Alia Therapeutics Srl | Compositions and methods for treatment of usher syndrome type 2a |
| CN119923466A (zh) * | 2022-06-15 | 2025-05-02 | 比姆医疗股份有限公司 | 用于减少补体激活的组合物和方法 |
| US20260014279A1 (en) * | 2022-09-16 | 2026-01-15 | Alia Therapeutics Srl | Enqp type cas proteins and applications thereof |
| KR20250153814A (ko) * | 2023-03-01 | 2025-10-27 | 메타지노미, 인크. | Ruvc 도메인을 갖는 효소 |
| WO2025106934A1 (en) * | 2023-11-16 | 2025-05-22 | Metagenomi, Inc. | Enzymes with ruvc domains |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2894684A1 (en) * | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes |
| EP3303634B1 (en) * | 2015-06-03 | 2023-08-30 | The Regents of The University of California | Cas9 variants and methods of use thereof |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| US12431216B2 (en) * | 2016-08-17 | 2025-09-30 | Broad Institute, Inc. | Methods for identifying class 2 crispr-cas systems |
| KR20200124702A (ko) * | 2018-02-23 | 2020-11-03 | 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 | 신규한 cas9 오르소로그 |
| AU2020223370B2 (en) | 2019-02-14 | 2023-04-20 | Metagenomi Therapeutics, Inc. | Enzymes with RuvC domains |
-
2020
- 2020-02-14 AU AU2020223370A patent/AU2020223370B2/en active Active
- 2020-02-14 WO PCT/US2020/018432 patent/WO2020168291A1/en not_active Ceased
- 2020-02-14 CN CN202080028787.6A patent/CN113728098A/zh active Pending
- 2020-02-14 KR KR1020217028874A patent/KR102623312B1/ko active Active
- 2020-02-14 CA CA3130135A patent/CA3130135C/en active Active
- 2020-02-14 JP JP2021547336A patent/JP2022520428A/ja not_active Ceased
- 2020-02-14 EP EP20756259.6A patent/EP3924482A4/en active Pending
- 2020-02-14 CA CA3241703A patent/CA3241703C/en active Active
- 2020-02-14 MX MX2021009886A patent/MX2021009886A/es unknown
- 2020-02-14 KR KR1020247000311A patent/KR20240007322A/ko active Pending
- 2020-02-14 CN CN202310441977.6A patent/CN116515797A/zh active Pending
-
2021
- 2021-08-16 MX MX2023006575A patent/MX2023006575A/es unknown
-
2023
- 2023-06-15 US US18/335,481 patent/US20240117330A1/en active Pending
- 2023-07-17 AU AU2023206079A patent/AU2023206079A1/en not_active Abandoned
- 2023-09-08 JP JP2023146414A patent/JP7502537B2/ja active Active
-
2024
- 2024-06-05 JP JP2024091759A patent/JP2024133476A/ja active Pending
-
2025
- 2025-12-11 AU AU2025279720A patent/AU2025279720A1/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| Cell 2014, feb 27; 156(5):935-949* |
| Scientific Reports. 2017; 7: 17271.* |
Also Published As
| Publication number | Publication date |
|---|---|
| CA3130135C (en) | 2024-11-19 |
| EP3924482A4 (en) | 2023-04-05 |
| JP2024133476A (ja) | 2024-10-02 |
| EP3924482A1 (en) | 2021-12-22 |
| JP7502537B2 (ja) | 2024-06-18 |
| MX2023006575A (es) | 2023-06-16 |
| AU2020223370A1 (en) | 2021-10-07 |
| JP2023179468A (ja) | 2023-12-19 |
| KR20210139254A (ko) | 2021-11-22 |
| AU2025279720A1 (en) | 2026-01-15 |
| KR20240007322A (ko) | 2024-01-16 |
| JP2022520428A (ja) | 2022-03-30 |
| WO2020168291A1 (en) | 2020-08-20 |
| MX2021009886A (es) | 2021-10-13 |
| US20240117330A1 (en) | 2024-04-11 |
| CA3130135A1 (en) | 2020-08-20 |
| AU2023206079A1 (en) | 2023-08-10 |
| CA3241703A1 (en) | 2020-08-20 |
| CN113728098A (zh) | 2021-11-30 |
| CA3241703C (en) | 2026-02-24 |
| AU2020223370B2 (en) | 2023-04-20 |
| CN116515797A (zh) | 2023-08-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102623312B1 (ko) | Ruvc 도메인이 존재하는 효소 | |
| US12024727B2 (en) | Enzymes with RuvC domains | |
| JP7778087B2 (ja) | Ｒｕｖｃドメインを有する酵素 | |
| US20220298494A1 (en) | Enzymes with ruvc domains | |
| US20240110167A1 (en) | Enzymes with ruvc domains | |
| US20220220460A1 (en) | Enzymes with ruvc domains | |
| EP4165177A1 (en) | Enzymes with ruvc domains | |
| US20250002881A1 (en) | Class ii, type v crispr systems | |
| GB2617659A (en) | Enzymes with RUVC domains | |
| HK40065084A (en) | Enzymes with ruvc domains |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PA0105 | International application |
Patent event date: 20210908 Patent event code: PA01051R01D Comment text: International Patent Application |
|
| PG1501 | Laying open of application | ||
| PN2301 | Change of applicant |
Patent event date: 20220414 Comment text: Notification of Change of Applicant Patent event code: PN23011R01D |
|
| A201 | Request for examination | ||
| A302 | Request for accelerated examination | ||
| PA0201 | Request for examination |
Patent event code: PA02012R01D Patent event date: 20230213 Comment text: Request for Examination of Application |
|
| PA0302 | Request for accelerated examination |
Patent event date: 20230213 Patent event code: PA03022R01D Comment text: Request for Accelerated Examination |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
Comment text: Notification of reason for refusal Patent event date: 20230620 Patent event code: PE09021S01D |
|
| E701 | Decision to grant or registration of patent right | ||
| PE0701 | Decision of registration |
Patent event code: PE07011S01D Comment text: Decision to Grant Registration Patent event date: 20231006 |
|
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
Comment text: Registration of Establishment Patent event date: 20240105 Patent event code: PR07011E01D |
|
| PR1002 | Payment of registration fee |
Payment date: 20240105 End annual number: 3 Start annual number: 1 |
|
| PG1601 | Publication of registration |