KR102607157B1 - 드노보 합성된 조합 핵산 라이브러리 - Google Patents

드노보 합성된 조합 핵산 라이브러리 Download PDF

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KR102607157B1
KR102607157B1 KR1020197029959A KR20197029959A KR102607157B1 KR 102607157 B1 KR102607157 B1 KR 102607157B1 KR 1020197029959 A KR1020197029959 A KR 1020197029959A KR 20197029959 A KR20197029959 A KR 20197029959A KR 102607157 B1 KR102607157 B1 KR 102607157B1
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variant
nucleic acid
delete delete
library
polynucleotides
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KR20190129081A (ko
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안토니 콕스
쓰위엔 천
찰스 레도가르
도미니크 토파니
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트위스트 바이오사이언스 코포레이션
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/10—Design of libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Library & Information Science (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
KR1020197029959A 2017-03-15 2018-03-14 드노보 합성된 조합 핵산 라이브러리 Active KR102607157B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020237040300A KR20230163591A (ko) 2017-03-15 2018-03-14 드노보 합성된 조합 핵산 라이브러리

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201762471723P 2017-03-15 2017-03-15
US62/471,723 2017-03-15
US201762578326P 2017-10-27 2017-10-27
US62/578,326 2017-10-27
PCT/US2018/022487 WO2018170164A1 (en) 2017-03-15 2018-03-14 De novo synthesized combinatorial nucleic acid libraries

Related Child Applications (1)

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KR1020237040300A Division KR20230163591A (ko) 2017-03-15 2018-03-14 드노보 합성된 조합 핵산 라이브러리

Publications (2)

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KR20190129081A KR20190129081A (ko) 2019-11-19
KR102607157B1 true KR102607157B1 (ko) 2023-11-27

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KR1020237040300A Ceased KR20230163591A (ko) 2017-03-15 2018-03-14 드노보 합성된 조합 핵산 라이브러리

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Country Status (11)

Country Link
US (1) US20180282721A1 (enExample)
EP (1) EP3596258A4 (enExample)
JP (2) JP7335165B2 (enExample)
KR (2) KR102607157B1 (enExample)
CN (2) CN117888207A (enExample)
AU (2) AU2018234624B2 (enExample)
CA (1) CA3056386A1 (enExample)
GB (1) GB2575576A (enExample)
IL (1) IL269288A (enExample)
SG (1) SG11201908489XA (enExample)
WO (1) WO2018170164A1 (enExample)

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WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
AU2016324296A1 (en) 2015-09-18 2018-04-12 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
CA3034769A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
CN110248724B (zh) 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
CN110366613A (zh) 2016-12-16 2019-10-22 特韦斯特生物科学公司 免疫突触的变体文库及其合成
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CN110913865A (zh) 2017-03-15 2020-03-24 特韦斯特生物科学公司 免疫突触的变体文库及其合成
AU2018234624B2 (en) * 2017-03-15 2023-11-16 Twist Bioscience Corporation De novo synthesized combinatorial nucleic acid libraries
IL271205B2 (en) 2017-06-12 2025-02-01 Twist Bioscience Corp Methods for assembling continuous nucleic acids
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
KR102637566B1 (ko) 2017-10-20 2024-02-16 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
CN112041438B (zh) 2018-01-04 2025-05-23 特韦斯特生物科学公司 基于dna的数字信息存储
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3124980A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
KR20210143766A (ko) * 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
CN113785057A (zh) 2019-02-26 2021-12-10 特韦斯特生物科学公司 用于抗体优化的变异核酸文库
JP2022550497A (ja) 2019-06-21 2022-12-02 ツイスト バイオサイエンス コーポレーション バーコードに基づいた核酸配列アセンブリ
CA3155629A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for crth2
AU2020355027A1 (en) * 2019-09-23 2022-04-21 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
CA3177029A1 (en) 2020-04-27 2021-11-04 Aaron Sato Variant nucleic acid libraries for coronavirus
US12391762B2 (en) 2020-08-26 2025-08-19 Twist Bioscience Corporation Methods and compositions relating to GLP1R variants
WO2022047076A1 (en) * 2020-08-28 2022-03-03 Twist Bioscience Corporation Devices and methods for synthesis
US11970697B2 (en) 2020-10-19 2024-04-30 Twist Bioscience Corporation Methods of synthesizing oligonucleotides using tethered nucleotides
EP4281112A4 (en) 2021-01-21 2025-01-15 Twist Bioscience Corporation METHODS AND COMPOSITIONS RELATING TO ADENOSINE RECEPTORS
US12258406B2 (en) 2021-03-24 2025-03-25 Twist Bioscience Corporation Antibodies that bind CD3 Epsilon
WO2022271884A2 (en) 2021-06-22 2022-12-29 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
AU2022390457A1 (en) 2021-11-18 2024-06-20 Twist Bioscience Corporation Dickkopf-1 variant antibodies and methods of use
WO2023130123A2 (en) 2022-01-03 2023-07-06 Twist Bioscience Corporation Bispecific sars-cov-2 antibodies and methods of use
US20230325641A1 (en) * 2022-04-07 2023-10-12 Winbond Electronics Corp. Light source optimization apparatus and light source optimization method
CN114694757B (zh) * 2022-04-20 2025-04-18 中南民族大学 Rna序列编码潜力预测方法及系统

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US20110082055A1 (en) 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
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US20110082055A1 (en) 2009-09-18 2011-04-07 Codexis, Inc. Reduced codon mutagenesis
WO2014035693A2 (en) 2012-08-31 2014-03-06 The Scripps Research Institute Methods and compositions related to modulators of eukaryotic cells
US20150038373A1 (en) 2013-08-05 2015-02-05 Twist Bioscience Corporation De novo synthesized gene libraries

Also Published As

Publication number Publication date
CN110914486B (zh) 2024-01-30
CA3056386A1 (en) 2018-09-20
KR20190129081A (ko) 2019-11-19
CN110914486A (zh) 2020-03-24
AU2018234624B2 (en) 2023-11-16
JP2020511135A (ja) 2020-04-16
JP7696937B2 (ja) 2025-06-23
EP3596258A4 (en) 2020-12-30
JP7335165B2 (ja) 2023-08-29
SG11201908489XA (en) 2019-10-30
CN117888207A (zh) 2024-04-16
IL269288A (en) 2019-11-28
KR20230163591A (ko) 2023-11-30
EP3596258A1 (en) 2020-01-22
AU2024201012A1 (en) 2024-06-06
GB2575576A (en) 2020-01-15
AU2018234624A1 (en) 2019-10-17
JP2023087685A (ja) 2023-06-23
US20180282721A1 (en) 2018-10-04
WO2018170164A1 (en) 2018-09-20
GB201914881D0 (en) 2019-11-27

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