KR102596125B1 - 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들 - Google Patents

표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들 Download PDF

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KR102596125B1
KR102596125B1 KR1020227002477A KR20227002477A KR102596125B1 KR 102596125 B1 KR102596125 B1 KR 102596125B1 KR 1020227002477 A KR1020227002477 A KR 1020227002477A KR 20227002477 A KR20227002477 A KR 20227002477A KR 102596125 B1 KR102596125 B1 KR 102596125B1
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tale
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3세 카를로스 에프. 바바스
앤드류 메르세르
브라이언 엠. 램
토마스 가이
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더 스크립스 리서치 인스티튜트
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020227002477A 2012-09-04 2013-09-04 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들 Active KR102596125B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261696689P 2012-09-04 2012-09-04
US61/696,689 2012-09-04
US201361753763P 2013-01-17 2013-01-17
US61/753,763 2013-01-17
US201361818364P 2013-05-01 2013-05-01
US61/818,364 2013-05-01
PCT/US2013/058100 WO2014039585A2 (en) 2012-09-04 2013-09-04 Chimeric polypeptides having targeted binding specificity
KR1020217000390A KR102357105B1 (ko) 2012-09-04 2013-09-04 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들

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KR20220016303A KR20220016303A (ko) 2022-02-08
KR102596125B1 true KR102596125B1 (ko) 2023-10-30

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KR1020157008001A Active KR102201867B1 (ko) 2012-09-04 2013-09-04 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들
KR1020227002477A Active KR102596125B1 (ko) 2012-09-04 2013-09-04 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들

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KR1020157008001A Active KR102201867B1 (ko) 2012-09-04 2013-09-04 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들

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US (4) US9902962B2 (https=)
EP (3) EP4148134A1 (https=)
JP (4) JP6442406B2 (https=)
KR (3) KR102357105B1 (https=)
CN (2) CN104781404B (https=)
AU (3) AU2013312801B2 (https=)
BR (1) BR112015004696B1 (https=)
CA (1) CA2883511C (https=)
DK (1) DK2893022T3 (https=)
IL (1) IL237442B (https=)
SG (2) SG10201701675PA (https=)
WO (1) WO2014039585A2 (https=)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010028347A2 (en) 2008-09-05 2010-03-11 President & Fellows Of Harvard College Continuous directed evolution of proteins and nucleic acids
CA2825370A1 (en) 2010-12-22 2012-06-28 President And Fellows Of Harvard College Continuous directed evolution
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
KR102357105B1 (ko) * 2012-09-04 2022-02-08 더 스크립스 리서치 인스티튜트 표적화된 바인딩 특이도를 갖는 키메라 폴리펩타이드들
US9708589B2 (en) * 2012-12-18 2017-07-18 Monsanto Technology Llc Compositions and methods for custom site-specific DNA recombinases
US10793867B2 (en) 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
WO2015134121A2 (en) 2014-01-20 2015-09-11 President And Fellows Of Harvard College Negative selection and stringency modulation in continuous evolution systems
WO2015153889A2 (en) 2014-04-02 2015-10-08 University Of Florida Research Foundation, Incorporated Materials and methods for the treatment of latent viral infection
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3194583B1 (en) * 2014-07-30 2021-09-15 Zumutor Biologics, Inc. Non-fucosylated protein and methods thereof
WO2016077052A2 (en) 2014-10-22 2016-05-19 President And Fellows Of Harvard College Evolution of proteases
WO2016168631A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Vector-based mutagenesis system
WO2017015545A1 (en) 2015-07-22 2017-01-26 President And Fellows Of Harvard College Evolution of site-specific recombinases
US11524983B2 (en) 2015-07-23 2022-12-13 President And Fellows Of Harvard College Evolution of Bt toxins
WO2017019895A1 (en) 2015-07-30 2017-02-02 President And Fellows Of Harvard College Evolution of talens
US11339408B2 (en) 2015-08-20 2022-05-24 Applied Stemcell, Inc. Nuclease with enhanced efficiency of genome editing
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018187796A1 (en) * 2017-04-07 2018-10-11 Donald Danforth Plant Science Center Methods for increasing resistance to cotton bacterial blight and plants produced thereby
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
US11447809B2 (en) 2017-07-06 2022-09-20 President And Fellows Of Harvard College Evolution of tRNA synthetases
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US12060553B2 (en) 2017-08-25 2024-08-13 President And Fellows Of Harvard College Evolution of BoNT peptidases
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
WO2019056002A1 (en) 2017-09-18 2019-03-21 President And Fellows Of Harvard College CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12312383B2 (en) 2018-04-18 2025-05-27 Altius Institute For Biomedical Sciences Animal pathogen-derived polypeptides and uses thereof for genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
WO2019241649A1 (en) 2018-06-14 2019-12-19 President And Fellows Of Harvard College Evolution of cytidine deaminases
US12264181B2 (en) 2018-06-27 2025-04-01 Altius Institute For Biomedical Sciences Nucleic acid binding domains and methods of use thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN109771403A (zh) * 2019-01-24 2019-05-21 广州医科大学附属第一医院 一种大鼠造影剂肾病模型的构建方法
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
US20210130828A1 (en) * 2019-11-01 2021-05-06 Sangamo Therapeutics, Inc. Gin recombinase variants
US12012433B1 (en) * 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
CN116096878A (zh) 2020-05-01 2023-05-09 合成Dna技术公司 在非经典tttt前间区序列邻近基序处具有增强的切割活性的毛螺菌科菌种cas12a突变体
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
US20230313154A1 (en) * 2020-07-17 2023-10-05 Cb Therapeutics, Inc. Prenyltransferase enzymes
KR102265501B1 (ko) * 2020-12-17 2021-06-15 국립해양생물자원관 다중구리 산화효소 펩타이드를 포함하는 구리대사 관련 신경계 질환의 예방 또는 치료용 조성물

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015501637A (ja) 2011-11-16 2015-01-19 サンガモ バイオサイエンシーズ， インコーポレイテッド 修飾されたｄｎａ結合タンパク質およびその使用
JP6442406B2 (ja) 2012-09-04 2018-12-19 ザ スクリプス リサーチ インスティテュート 標的結合特異性を有するキメラポリペプチド

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993025682A2 (en) * 1992-06-11 1993-12-23 New York University Chimeric receptor polypeptides, human h13 proteins and uses thereof
US20050084885A1 (en) 1994-01-18 2005-04-21 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6140466A (en) 1994-01-18 2000-10-31 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US6242568B1 (en) 1994-01-18 2001-06-05 The Scripps Research Institute Zinc finger protein derivatives and methods therefor
US7277941B2 (en) 1998-03-11 2007-10-02 Commvault Systems, Inc. System and method for providing encryption in a storage network by storing a secured encryption key with encrypted archive data in an archive storage device
US6140081A (en) 1998-10-16 2000-10-31 The Scripps Research Institute Zinc finger binding domains for GNN
US7013219B2 (en) 1999-01-12 2006-03-14 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6453242B1 (en) 1999-01-12 2002-09-17 Sangamo Biosciences, Inc. Selection of sites for targeting by zinc finger proteins and methods of designing zinc finger proteins to bind to preselected sites
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US6599692B1 (en) 1999-09-14 2003-07-29 Sangamo Bioscience, Inc. Functional genomics using zinc finger proteins
US6794136B1 (en) 2000-11-20 2004-09-21 Sangamo Biosciences, Inc. Iterative optimization in the design of binding proteins
US7030215B2 (en) 1999-03-24 2006-04-18 Sangamo Biosciences, Inc. Position dependent recognition of GNN nucleotide triplets by zinc fingers
AU4940700A (en) 1999-05-28 2000-12-18 Sangamo Biosciences, Inc. Gene switches
AU776576B2 (en) 1999-12-06 2004-09-16 Sangamo Biosciences, Inc. Methods of using randomized libraries of zinc finger proteins for the identification of gene function
US7067317B2 (en) 2000-12-07 2006-06-27 Sangamo Biosciences, Inc. Regulation of angiogenesis with zinc finger proteins
AU2002228841C1 (en) 2000-12-07 2006-11-23 Sangamo Biosciences, Inc Regulation of angiogenesis with zinc finger proteins
WO2002057293A2 (en) 2001-01-22 2002-07-25 Sangamo Biosciences, Inc. Modified zinc finger binding proteins
US7067617B2 (en) 2001-02-21 2006-06-27 The Scripps Research Institute Zinc finger binding domains for nucleotide sequence ANN
JP2005500061A (ja) 2001-08-20 2005-01-06 ザ スクリップス リサーチ インスティテュート Ｃｎｎについての亜鉛フィンガー結合ドメイン
US20060078880A1 (en) 2002-02-07 2006-04-13 The Scripps Research Institute Zinc finger libraries
US20060211846A1 (en) 2002-02-13 2006-09-21 Barbas Carlos F Iii Zinc finger binding domains for nucleotide sequence ANN
US8685687B2 (en) * 2006-07-05 2014-04-01 The Scripps Research Institute Chimeric zinc finger recombinases optimized for catalysis by directed evolution
US8586526B2 (en) 2010-05-17 2013-11-19 Sangamo Biosciences, Inc. DNA-binding proteins and uses thereof
WO2011072246A2 (en) * 2009-12-10 2011-06-16 Regents Of The University Of Minnesota Tal effector-mediated dna modification
CN103210087B (zh) * 2010-09-06 2015-04-29 淡马锡生命科学研究院有限公司 Xa10与AvrXa10之间的分子相互作用
US9499592B2 (en) * 2011-01-26 2016-11-22 President And Fellows Of Harvard College Transcription activator-like effectors
WO2013101877A2 (en) * 2011-12-29 2013-07-04 Iowa State University Research Foundation, Inc. Genetically modified plants with resistance to xanthomonas and other bacterial plant pathogens

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015501637A (ja) 2011-11-16 2015-01-19 サンガモ バイオサイエンシーズ， インコーポレイテッド 修飾されたｄｎａ結合タンパク質およびその使用
JP6442406B2 (ja) 2012-09-04 2018-12-19 ザ スクリプス リサーチ インスティテュート 標的結合特異性を有するキメラポリペプチド

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Nat Biotechnol.,29(2):143-148(2010.12.22.)
Nucleic Acids Res.,39(21):9283-9293(2011.8.3.)

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US20180230474A1 (en) 2018-08-16
AU2021240252A1 (en) 2021-10-28
CN104781404B (zh) 2018-10-26
AU2013312801A1 (en) 2015-03-26
JP2018183180A (ja) 2018-11-22
EP2893022A4 (en) 2016-03-02
US11466277B2 (en) 2022-10-11
WO2014039585A3 (en) 2014-05-22
JP2020178703A (ja) 2020-11-05
KR20220016303A (ko) 2022-02-08
WO2014039585A2 (en) 2014-03-13
EP3750999B1 (en) 2022-06-29
EP3750999A1 (en) 2020-12-16
CN104781404A (zh) 2015-07-15
IL237442A0 (en) 2015-04-30
CN109517068A (zh) 2019-03-26
US20230304022A1 (en) 2023-09-28
EP2893022A2 (en) 2015-07-15
JP2022130649A (ja) 2022-09-06
KR102357105B1 (ko) 2022-02-08
US9902962B2 (en) 2018-02-27
US20150284728A1 (en) 2015-10-08
US20210147854A1 (en) 2021-05-20
BR112015004696A2 (pt) 2017-11-21
AU2019200922A1 (en) 2019-02-28
CN109517068B (zh) 2023-07-25
KR20150070120A (ko) 2015-06-24
SG11201501525QA (en) 2015-03-30
JP6442406B2 (ja) 2018-12-19
CA2883511A1 (en) 2014-03-13
AU2013312801B2 (en) 2018-11-15
BR112015004696B1 (pt) 2022-07-19
DK2893022T3 (da) 2020-07-27
JP7459174B2 (ja) 2024-04-01
IL237442B (en) 2019-11-28
EP4148134A1 (en) 2023-03-15
SG10201701675PA (en) 2017-04-27
KR102201867B1 (ko) 2021-01-12
AU2019200922B2 (en) 2021-07-08
JP6728283B2 (ja) 2020-07-22
JP2015532654A (ja) 2015-11-12
JP7100681B2 (ja) 2022-07-13
KR20210008437A (ko) 2021-01-21
CA2883511C (en) 2024-05-07
US10829766B2 (en) 2020-11-10
AU2021240252B2 (en) 2024-06-06
EP2893022B1 (en) 2020-05-13

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