KR102579228B1 - Automatic System for Urine Analysis - Google Patents

Abstract

The present invention relates to a body fluid and reagent mixing device for an automatic body fluid analysis system that can perform the analysis function of body fluids by mixing reagents and body fluids in the reagent chamber of a body fluid analysis kit. The body fluids of the body fluid automatic analysis system according to the present invention and a reagent mixing device, a kit for analyzing body fluids; A loading plate on which the kit body of the body fluid analysis kit is seated when the kit body and the kit cover are separated; a body fluid administration unit that administers a certain amount of body fluid of the test subject to the reagent chamber of the kit body on the loading plate; The loading plate is positioned from a loading position where the kit body is seated on the loading plate to a bodily fluid administration position where the body fluid is administered by the body fluid administration unit, a kit assembly position where the kit cover of the body fluid analysis kit is coupled to the upper part of the kit body, and The body fluid analysis kit is electrically connected to the probe terminal of the connection part and continuously transferred to the connection position where body fluid analysis is performed, and the loading plate is installed between the body fluid administration position and the kit assembly position, or between the kit assembly position and the connection position. It may include a transfer and stirring unit that agitates the reagents and body fluids in the reagent chamber by repeatedly moving back and forth at a certain distance and a certain number of times to vibrate the kit body on the loading plate.

Description

Body fluid and reagent mixing device for automatic body fluid analysis system {Automatic System for Urine Analysis}

The present invention relates to a bodily fluid analysis system for analyzing body fluids such as urine. More specifically, when a kit for body fluid analysis and a body fluid container storing body fluids (e.g. urine or blood, etc.) of a test subject are introduced, the body fluid inside the body fluid container is Bodily fluid is automatically administered to the kit's reagent, the biosensor is immersed in the body fluid-administered reagent, the kit is transported to the test location, and the probe terminal is electrically connected to the kit's biosensor at the test location to determine the components of the body fluid. It relates to a bodily fluid and reagent mixing device in an automatic bodily fluid analysis system that stirs the reagents and body fluids to be uniformly mixed during the process of administering and transferring body fluids to a kit in an automatic body fluid analysis system that can automatically analyze.

A urine analyzer is a body fluid analyzer used to diagnose diseases, prevent and treat diseases by analyzing the contents of urine discharged outside the human body.

Test items that can be diagnosed through urine analysis include sugar, ketone bodies, occult blood, white blood cells, urobilinogen, bilirubin, protein, nitrite, acidity and specific gravity. Here, sugar can predict diabetes, nephrogenic diabetes, and enterocolitis, and ketone bodies can diagnose diseases such as diabetes, febrile diseases, and occult blood, such as cystitis, urinary bleeding, and hemolytic anemia. In addition, white blood cells predict kidney disease and pyelonephritis, urobilinogen and bilirubin predict jaundice, liver disease, and biliary tract disease, protein predict nephritis, cystitis, and high blood pressure, and nitrite and acidity predict urinary tract infections. It is known that diabetic ketosis, hunger and vomiting can be diagnosed through specific gravity.

Since many diseases can be diagnosed through urine analysis, recently, urine analyzers that can automatically analyze urine and diagnose diseases using various methods have been developed.

A urine analyzer that has been used conventionally includes a urine analyzer disclosed in Domestic Patent Registration No. 10-1213221. The urine analyzer of the registered patent captures the urine-stained diagnostic reagent strip with an LED light and an image sensor, detects color changes due to the chemical reaction of the diagnostic reagent strip, and acquires urine test measurement data according to the changed color image information. It was made so that it could be done.

However, urine analyzers using diagnostic reagent strips have the disadvantage that it is difficult to analyze the urine of multiple test subjects at once, and the analysis and diagnosis of urine components are very limited.

In addition, there is a high possibility of urine staining the user's body during the urine analysis process, so there is a high possibility of infection through urine.

Republic of Korea Patent No. 10-1213221 (registered on December 11, 2012) Republic of Korea Patent No. 10-1685086 (registered on December 5, 2016) Republic of Korea Patent No. 10-0522722 (registered on October 12, 2005)

The present invention is to solve the above problems, and the purpose of the present invention is to administer the user's body fluid to the reagent of the kit, transport the kit to the test location, and connect the biosensor of the kit to the probe terminal at the test position. A series of processes to analyze the components of body fluids can be performed automatically and continuously on a single device, and the accuracy of analysis is improved by stirring to ensure that the reagents and body fluids are evenly mixed during the process of administering and transporting body fluids to the kit. The purpose is to provide a body fluid and reagent mixing device for an automatic body fluid analysis system.

The body fluid and reagent mixing device of the automatic body fluid analysis system according to the present invention for achieving the above object includes a kit body in which a plurality of reagent chambers in which reagents are stored are formed to be open upward, and a test subject being placed in the reagents of the reagent chambers. A kit cover coupled to the top of the kit body after a certain amount of bodily fluid is administered, a plurality of sensor connectors installed on the kit cover to correspond to the reagent chamber, and coupled to each of the sensor connectors so that the kit cover is connected to the kit body. A kit for body fluid analysis including a plurality of biosensors that are inserted into the reagent chamber and immersed in a reagent when coupled to the upper part; A loading plate on which the kit body of the body fluid analysis kit is seated when the kit body and the kit cover are separated; a body fluid administration unit that administers a certain amount of body fluid of the test subject to the reagent chamber of the kit body on the loading plate; The loading plate is positioned from a loading position where the kit body is seated on the loading plate to a bodily fluid administration position where the body fluid is administered by the body fluid administration unit, a kit assembly position where the kit cover of the body fluid analysis kit is coupled to the upper part of the kit body, and The body fluid analysis kit is electrically connected to the probe terminal of the connection part and continuously transferred to the connection position where body fluid analysis is performed, and the loading plate is installed between the body fluid administration position and the kit assembly position, or between the kit assembly position and the connection position. It may include a transfer and stirring unit that agitates the reagents and body fluids in the reagent chamber by repeatedly moving back and forth at a certain distance and a certain number of times to vibrate the kit body on the loading plate.

The transfer and stirring unit consists of a fixed unit installed to extend to the loading position, the body fluid administration position, the kit assembly position, and the connection position, and a movable unit movably installed on the fixed unit and coupled to the loading plate at the upper end. Including a linear motion module, the movable unit can vibrate the kit body on the loading plate by horizontally reciprocating with respect to the fixed unit.

A kit guide groove supported as the lower end of the kit body is inserted into the upper surface of the loading plate may be formed concave downward.

The body fluid administration unit includes a multi-joint manipulator configured to move in any direction, and a negative pressure system installed on the multi-joint manipulator to suck body fluid from the body fluid container and discharge the body fluid from the upper side of the reagent chamber of the kit body. ) and a syringe pump unit that generates positive pressure, a pipette standby unit in which a plurality of unused pipettes that are detachably installed at the tip of the syringe pump unit standby, and a pipette standby unit that is coupled to the syringe pump unit for use. It may include a pipette disposal unit that collects pipettes separated from the syringe pump unit after completion.

Before seating the kit body on the loading plate, the kit cover is detachably coupled to the lower part of the kit body and transported, and when seating the kit body on the loading plate, the kit cover is separated from the lower part of the kit body. It is turned over and put into the automatic bodily fluid analysis system, and with the kit cover coupled to the lower part of the kit body, the upper part of the reagent chamber of the kit body is sealed by a temporary sealing material in the form of a film that is detachably attached to the upper surface of the kit body. , the kit body is made in the form of a box with an open lower surface, and a sensor receiving groove into which the biosensor is inserted may be formed on the outer side of the reagent chamber when the kit cover is coupled to the lower part of the kit body.

When the kit cover is coupled to the upper part of the kit body at the kit assembly position and the biosensor is inserted into the reagent chamber, the biosensor may be placed in a position eccentric in one direction from the central axis of the reagent chamber.

The kit for bodily fluid analysis may further include a locking unit that restrains the kit cover to the kit body so that the kit cover is not separated from the kit body after the kit cover is coupled to the upper part of the kit body at the kit assembly position.

According to the present invention, the analysis function of body fluids can be performed by mixing reagents and body fluids in the reagent chamber of a body fluid analysis kit, thereby further improving the accuracy of body fluid analysis.

In particular, after administering bodily fluids to the reagent stored in the kit body of the body fluid analysis kit, in the process of transferring the kit body to the transport and stirring unit, the transport and stirring unit repeatedly moves the kit main body back and forth at a certain distance and a certain number of times to load the loading plate. By vibrating the upper kit body, the reagents and body fluids in the reagent chamber can be stirred and mixed, so the mixing of the reagents and body fluids can be more uniform, and the accuracy of analysis can be further improved.

Figure 1 is a perspective view showing the external appearance of an automatic bodily fluid analysis system to which a bodily fluid and reagent mixing device is applied according to an embodiment of the present invention.
FIG. 2 is a plan view of the automatic bodily fluid analysis system shown in FIG. 1.
FIG. 3 is a front cross-sectional view of the automatic bodily fluid analysis system shown in FIG. 1.
Figure 4 is a perspective view showing an example of a kit for analyzing body fluids applied to the automatic body fluid analysis system shown in Figure 1.
Figures 5a and 5b are exploded perspective views of the kit for analyzing body fluids shown in Figure 4, respectively.
Figure 6 is a bottom perspective view of the kit body constituting the kit for body fluid analysis shown in Figure 4.
Figure 7 is a bottom perspective view of the kit cover constituting the kit for bodily fluid analysis shown in Figure 4.
FIGS. 8 and 9 are cross-sectional views of the kit for body fluid analysis shown in FIG. 4, FIG. 8 is a cross-sectional view in a state of transport before body fluid analysis, and FIG. 9 is a cross-sectional view in a state in which it is inserted into an automatic body fluid analysis system for body fluid analysis. am.
Figure 10 is a front view showing the main configuration of the body fluid administration unit that constitutes the automatic body fluid analysis system shown in Figure 1.
FIG. 11 is an exploded perspective view showing a partial configuration of the transfer and stirring unit that constitutes the automatic bodily fluid analysis system shown in FIG. 1.
Figure 12 is a front view showing the main components of the kit assembly part constituting the automatic bodily fluid analysis system shown in Figure 1.
Figures 13a and 13b are side views showing the main configuration and operation example of the connection part constituting the automatic bodily fluid analysis system shown in Figure 1.

The embodiments described in this specification and the configurations shown in the drawings are only preferred examples of the disclosed invention, and at the time of filing this application, there may be various modifications that can replace the embodiments and drawings in this specification.

Hereinafter, with reference to the attached drawings, the automatic bodily fluid analysis system will be described in detail according to the embodiments described below.

1 to 3 show the configuration of an embodiment of an automatic bodily fluid analysis system to which the bodily fluid and reagent mixing device according to the present invention is applied. The automatic bodily fluid analysis system 100 includes a kit for bodily fluid analysis (1) and a main body ( 101), a kit body loading unit 110 configured in the main body 101, a kit cover loading unit 115, a container handling unit 120, a bodily fluid injection unit 140, a kit assembly unit 150, and a connection unit ( 160), a control unit 170, a kit unloading unit 118, a container unloading unit 126, a transfer and stirring unit 130, etc.

The body fluid analysis kit (1) is a component of the body fluid and reagent mixing device of the present invention. Referring to FIGS. 4 to 9, the body fluid analysis kit (1) includes a plurality of reagent chambers in which reagents (L) are stored. After a certain amount of the test subject's body fluid (e.g. urine) is administered to the kit body (10) in which (11) is formed to be open to the upper side, and the reagent (L) of the reagent chamber (11), the kit body (10) A kit cover 20 coupled to the top, a plurality of sensor connectors 30 installed on the kit cover 20 to correspond to the reagent chamber 11, and each of the sensor connectors 30 are coupled to the kit. When the cover 20 is coupled to the upper part of the kit body 10, it includes a plurality of biosensors 40 that are inserted into the reagent chamber 11 and immersed in the reagent.

The kit body 10 has a substantially square box shape with an open lower surface, and a plurality of reagent chambers 11 in which reagents (L) for body fluid analysis are stored are formed to be open upward. The number of reagent chambers 11 may vary depending on the type of reagent and the number of test subjects. In this embodiment, the reagent chamber 11 of the kit body 10 is arranged horizontally and vertically in 4 × 3 so that the body fluids (urine) of four test subjects can be analyzed by injecting three types of reagents (L) at once. . The reagent chamber 11 has a rectangular cross-section, but may also have various cross-sectional shapes such as circular, oval, or track shapes. In addition, the reagent chamber 11 may have a constant cross-sectional area from the lower end to the upper end, but in order to ensure that the reagent L and the body fluid are uniformly mixed after the body fluid is injected into the reagent chamber 11, the cross-sectional area increases from the lower end to the upper end. It can have a tapered shape with a gradually increasing area.

The kit body 10 may have a box shape with an open lower surface so that the side portion of the kit cover 20 can be inserted and coupled. In order to allow the biosensor 40 to be accommodated inside the kit body 10 without being immersed in the reagent (L) when the kit cover 20 is coupled to the lower part of the kit body 10, the kit body ( A sensor receiving groove 13 into which the biosensor 40 is inserted is formed on the outer side of the reagent chamber 11 in the lower space of 10). The sensor receiving groove 13 is installed between the outer surface of each reagent chamber 11 and between one side of the reagent chamber 11 disposed on one outer side and the kit body 10, and the reagent chamber 11 In the same way, it is arranged horizontally and vertically in 4×3.

The kit cover 20 is detachably coupled to the lower part of the kit body 10 before being put into the automatic body fluid analysis system 100, and is attached to the kit body when it is put into the body fluid automatic analysis system 100 for body fluid analysis. After being separated from the lower part of (10), it is turned over and put into the automatic bodily fluid analysis system (100), and is then coupled to the upper part of the kit body (10) at the kit assembly position within the automatic bodily fluid analysis system (100). When the kit cover 20 is coupled to the lower part of the kit body 10, the upper part of the reagent chamber 11 of the kit body 10 is a temporary film-type detachable attachment to the upper surface of the kit body 10. It is sealed by the sealing material 50 to prevent the reagent L from flowing out during the process of transporting or handling the body fluid analysis kit 1. The temporary sealant 50 may be made of a thin resin film adhered to the upper surface of the kit body 10. In addition, the lower surface of the temporary sealing material 50 may be made of a hydrophobic surface to prevent reagents from being smeared.

The kit cover 20 is made in the form of a square box with an open lower surface, and a plurality of sensor connectors 30 are installed on the upper surface of the kit cover 20 to correspond to the reagent chamber 11. Therefore, in this embodiment, the sensor connector 30 is arranged horizontally and vertically in a 4x3 arrangement, the same as the reagent chamber 11. Each sensor connector 30 has a connection pin made of a conductive metal that is electrically connected to the probe terminal 162 (see FIG. 13A) of the connection part 160 (see FIG. 2) included in the automatic bodily fluid analysis system 100. 31) is provided. A biosensor 40 is inserted and connected to the lower part of each sensor connector 30.

The biosensor 40 is in the form of a rectangular strip that is long in the vertical direction, and its upper end is inserted into the sensor connector 30 and electrically connected to the connection terminal of the sensor connector 30. The biosensor 40 is provided with a sensing unit 41 in the central portion equipped with a bioreceptor that detects target molecules in body fluid. The biosensor 40 can be constructed by applying a known biosensor. When the kit cover 20 is coupled to the lower part of the kit body 10, the biosensor 40 is inserted into the sensor receiving groove 13 formed in the lower space of the kit body 10, and the kit cover 20 is turned over. When coupled to the upper part of the kit body 10, it is inserted into the reagent chamber 11 of the kit body 10 and immersed in the reagent (L). When the biosensor 40 is immersed in the reagent (L), the central sensing part 41 is in sufficient contact with the reagent (L) and body fluid to enable smooth detection of target molecules. Can be arranged in a position eccentric by a certain distance (d) in one direction from the central axis of the reagent chamber 11.

Referring again to FIGS. 1 to 3 and 10 to 13B, the automatic bodily fluid analysis system 100 includes a kit body loading unit 110 into which the kit body 10 of the body fluid analysis kit 1 is inserted, and a body fluid analysis system 100. A kit cover loading unit 115 into which the kit cover 20 of the kit (1) is inserted, a container handling unit 120 into which the bodily fluid container 2 storing the body fluid (urine) of the test subject is introduced, and a container handling unit 120. ), a bodily fluid administration unit 140 for extracting a certain amount of bodily fluid from the bodily fluid container 2 introduced through the kit body loading unit 110 and administering it to the reagent chamber 11 of the kit body 10 introduced through the kit body loading unit 110; After injecting the body fluid into the reagent chamber 11 of (10), the kit cover 20 introduced from the kit cover loading unit 115 is coupled to the upper part of the kit body 10 to produce the body fluid analysis kit (1). A kit assembly unit 150 to be assembled, a connection unit provided with a probe terminal 162 that is approached from the upper side of the body fluid analysis kit 1 transferred from the kit assembly unit 150 and is electrically connected to the sensor connector 30. (160), which is electrically connected to the connection unit 160 and has an analysis program that analyzes body fluid components based on the signal transmitted from the biosensor 40 of the body fluid analysis kit (1) and automatically analyzes body fluids. A control unit 170 that controls the operation of various components of the system 100, a kit unloading unit that discharges the body fluid analysis kit (1) to the outside after the analysis of the components of the body fluid administered to the body fluid analysis kit (1) is completed. Part 118, a container unloading part 126 for discharging the body fluid container to the outside after completion of use of the body fluid container 2, the body fluid is loaded from the loading position of the kit body 10 inserted into the kit body loading unit 110. Continuous horizontal transfer to the bodily fluid administration position (P1) by the administration unit 140, the kit assembly position (P2) by the kit assembly unit 150, and the connection position (P3) by the connection unit 160, and Includes a stirring unit 130.

The kit body loading part 110 is a part into which the kit body 10 of the body fluid analysis kit 1 is inserted. The kit body 101 makes it easy for the examiner to insert the kit body 10 into the body 101 ) is placed on the anterior side of the The kit body loading unit 110 includes a loading plate 111 on which at least one kit body 10 is mounted. The loading plate 111 is coupled to the transfer and agitation unit 130 and is moved by the transfer and agitation unit 130 to a loading position, a body fluid administration position (P1), a kit assembly position (P2), and a connection position (P3). It is configured to move continuously. Here, the loading position refers to a position where the kit body 10 is seated on the loading plate 111 and is a position corresponding to the kit body loading part 110.

The kit cover loading part 115 is a part where the kit cover 20 of the body fluid analysis kit 1 is inserted, and the main body 101 makes it easy for the examiner to insert the kit body 10 into the main body 101. ) is placed on the anterior side of the A cover seating plate 116 on which the kit cover 20 is seated and waiting is installed in the kit cover loading unit 115.

The container handling unit 120 is provided on one side of the main body 101, and a plurality of bodily fluid containers 2 storing the bodily fluids of a plurality of test subjects are inserted. The body fluid container 2 has a lid at the top, and when the lid is turned, the entrance at the top of the body fluid container 2 opens, allowing the body fluid to be extracted. Additionally, a barcode or QR code with a unique number may be attached to the bodily fluid container 2 to identify the bodily fluid of the test subject. The container handling unit 120 includes a container transfer unit 122 that transports the bodily fluid container 2 a certain distance, and the container transfer unit 122 holds the lid of the bodily fluid container 2 and rotates it in one direction to open the lid. A container opening and closing unit (125) that separates and reconnects the lid of the bodily fluid container from which withdrawal of bodily fluid has been completed to the bodily fluid container (2), and identifies the unique number of the bodily fluid container (2) immediately before extraction of the bodily fluid by the bodily fluid administering unit (140). It may include a unique number acquisition unit (not shown) that acquires and transmits the number to the control unit 170.

The container unloading unit 126 is installed in communication with the container transfer passage (not shown) on the lower side of the container handling unit 120, and when the bodily fluid container 2 from which bodily fluid extraction is completed falls through the container transfer passage, the body fluid Receive container (2) and store it temporarily.

The body fluid administration unit 140 extracts a certain amount of body fluid from the body fluid container 2 with the lid open from the container handling unit 120 and then waits at the body fluid administration position P1 on the transfer and stirring unit 130. It is configured so that body fluid can be divided and administered in predetermined amounts to a plurality of reagent chambers 11 of the kit body 10. In this embodiment, the body fluid injection unit 140 includes a multi-joint manipulator 141 configured to move in any direction, and is installed on the multi-joint manipulator 141 to suck body fluid from the body fluid container 2. A syringe pump unit 142 that generates negative pressure and positive pressure to discharge body fluid from the upper side of the reagent chamber 11 of the kit body 10, and a separation unit at the tip of the syringe pump unit 142. A pipette standby unit 144 in which a plurality of unused pipettes 143 are installed, and a pipette 143 that is coupled to the syringe pump unit 142 and separated from the syringe pump unit 142 after completion of use. ) includes a pipette disposal unit 145 that collects.

The articulated manipulator 141 can be configured by applying a manipulator applied to known industrial articulated robots. Additionally, the syringe pump unit 142 is installed on the end plate of the multi-joint manipulator 141 and suctions and discharges a predetermined amount of body fluid through a pipette 143 coupled to the tip. The syringe pump unit 142 can be configured by applying a Hamilton syringe pump capable of precise and continuous dispensing of fluid.

The transfer and stirring unit 130 performs the function of sequentially transferring the loading plate 111 to a designated location, and also repeatedly moves the loading plate 111 back and forth at a certain distance and a certain number of times during the transfer process to It is configured to perform the function of stirring and mixing the reagent and body fluid in the reagent chamber 11 by vibrating the kit body 10 at (111).

The transfer and stirring unit 130 includes a fixing unit 131 installed to extend to the loading position, the body fluid administration position (P1), the kit assembly position (P2), and the connection position (P3), and the fixing unit 131. It is movably installed and may include a linear motion module composed of a movable unit 132 to which the loading plate 111 is coupled to the upper end. In this embodiment, the linear motion module is configured by applying a linear motion system (LMS) using a linear motor, and the fixing unit 131 is a stator of a linear motor and an LM guide. It is configured, and the movable unit 132 may be configured as a mover of a linear motor. In addition, when a linear motion system using a servomotor and a ball screw is applied as a linear motion module, the servomotor, the ball screw rotated by the servomotor, and the LM guide become a fixed unit 131, and are coupled to the outer surface of the ball screw. The nut portion that moves along the ball screw and LM guide by rotation of the ball screw can become the movable unit 132. In addition, a linear motion module can be configured by applying various known linear motion systems.

The movable unit 132 of the transfer and stirring unit 130 is located between the body fluid administration position (P1) and the kit assembly position (P2) or the kit assembly position during the process of transporting the loading plate 111 and the kit body 10. The reagent chamber (11) is moved between (P2) and the connection position (P3) by repeatedly horizontally moving the loading plate (111) back and forth at a certain distance and a certain number of times to vibrate the kit body (10) on the loading plate (111) in one direction. Ensure that the reagents and body fluids inside are well stirred and mixed. If there is no stirring action by the transfer and stirring unit 130, the reagents and body fluids stored in the reagent chamber 11 of the kit body 10 are not properly mixed due to the density difference, resulting in accurate analysis during the subsequent body fluid analysis process. Although there is a possibility that it may not be lost, as in the present invention, if the loading plate 111 is horizontally moved back and forth a small distance and vibrated in the transfer and stirring unit 130, the reagents and body fluids are mixed smoothly, greatly improving the accuracy of analysis. You can get the benefits.

The kit assembly unit 150 is configured to assemble the kit 1 for body fluid analysis by coupling the kit cover 20 to the upper part of the kit body 10 into which the body fluid has been administered. As shown in FIG. 12, the kit is assembled. A cover transfer unit 151 installed horizontally and vertically movable above the position P2 and above the kit cover loading unit 115, and a kit cover loading unit 115 installed on the cover transfer unit 151. ) includes a cover gripper 152 that grips the kit cover 20. The cover gripper 152 opens and closes left and right by a known linear motion system such as a pneumatic cylinder, grips both sides of the kit cover 20, and transfers it to the kit assembly position (P2). ) is configured to press and couple the kit cover 20 to the upper part of the kit body 10.

The connection unit 160 is connected to the body fluid analysis kit (1) when the body fluid analysis kit (1) assembled at the kit assembly position (P2) is transferred to the lower side of the connection unit 160 by the transfer and stirring unit 130 and aligned. ) is electrically connected to the sensor connector 30 to perform body fluid analysis work. As shown in Figures 13a and 13b, the connection part 160 is a probe installed to be movable up and down on the main body 101. A holder 161, a plurality of probe terminals 162 installed on the probe holder 161 and electrically connected to the sensor connector 30 of the body fluid analysis kit (1) when the probe holder 161 is lowered, and a lifting unit that moves the probe holder 161 up and down.

The probe holder 161 has a substantially square frame shape, is connected to the lifting unit, and moves back and forth up and down along the lifting rail 164 provided at the connection part 160. A PCB board 132 on which a plurality of probe terminals 162 electrically connected to the sensor connector 30 are arranged is installed in the probe holder 161. The probe terminal 162 is electrically connected to the control unit 140 to transmit and receive electrical signals.

The lifting unit is a component that generates power to move the probe holder 161 in the vertical direction, and can be constructed using a known linear motion device including a motor and a ball screw rotated by the motor. there is.

The control unit 140 is electrically connected to the PCB board 132 of the connection unit 160 through a wire to transmit and receive electrical signals, and determines body fluid components based on the electrical signal transmitted from the biosensor 40 through the connection unit 160. There is a built-in analysis program that automatically analyzes. In addition, the control unit 140 also performs the function of controlling the operation of various components constituting the connection unit 160, transfer and stirring unit 130, body fluid injection unit 140, and kit assembly unit 150.

The kit unloading unit 118 is disposed near the connection unit 160 so that the body fluid analysis kit 1, which has been analyzed in the connection unit 160, can be discharged to the outside of the main body 101. ) is placed in front of the

Meanwhile, on the front side of the main body 110, there is a display panel ( 190) can be installed.

The operation of the automatic bodily fluid analysis system 100 configured as described above will be described in detail as follows.

An examiner (operator) places the kit body 10 of the body fluid analysis kit 1 on the loading plate 111 of the kit body loading unit 110. In this embodiment, three kit bodies 10 are mounted in parallel on the loading plate 111. Then, the inspector seats the kit cover 20 separated from the lower part of the kit body 10 on the cover seating plate 116 of the kit cover loading part 115. Additionally, the examiner inserts a plurality of bodily fluid containers (2) in which the body fluids of the test subject are stored into the container handling unit (120).

In this way, the kit body (10), kit cover (20), and body fluid container (2) of the body fluid analysis kit (1) are put into the body part (101) to prepare for analysis, and the body fluid automatic analysis system (100) is started. When the kit body 10 is seated, the loading plate 111 is moved horizontally by the linear motion system (LMS), which is the transfer and stirring unit 130, to the body fluid administration position where the body fluid is administered by the body fluid injection unit 140. Wait at (P1).

At this time, the container handling unit 120 sequentially transfers the bodily fluid container 2 to the lower side of the container opening and closing unit 125 using the container transfer unit 122. When the bodily fluid container (2) is aligned with the container opening and closing unit (125), the container opening and closing unit (125) holds the lid of the bodily fluid container (2) and turns it in one direction to separate the lid and open the upper entrance portion of the bodily fluid container (2). do.

Then, the multi-joint manipulator 141 of the body fluid injection unit 140 aligns the syringe pump unit 142 on the pipette standby unit 144 and then descends to couple the pipette 143 to the distal end of the syringe pump unit 142. Then, it moves toward the container opening and closing unit 125 of the container handling unit 120. Next, the tip of the pipette 143 is immersed in the bodily fluid container 2 with an open upper inlet, and then a predetermined amount of bodily fluid is sucked into the pipette 143 using the syringe pump unit 142.

The multi-joint manipulator 141 injects a certain amount of body fluid at a time into the plurality of reagent chambers 11 of the kit body 10, where the pipette 143 is waiting at the body fluid administration position P1.

When administration of the body fluid is completed at the body fluid administration position (P1), the loading plate 111 moves horizontally to the kit assembly position (P2) by the transfer and stirring unit 130 and waits. At this time, the transfer and agitation unit 130 repeatedly horizontally moves the loading plate 111 back and forth at a certain distance and a certain number of times between the body fluid administration position (P1) and the kit assembly position (P2) to form a loading plate ( 111) By vibrating the kit body 10 in one direction, the reagents and body fluids in the reagent chamber 11 can be well agitated.

When the kit body 10 stops and waits at the kit assembly position (P2), the cover gripper 152 of the kit assembly part 150 waits on the cover seating plate 116 of the kit cover loading part 115. Grasp both sides of (20) and move to the kit assembly position (P2), then assemble the body fluid analysis kit (1) by attaching the kit cover (20) to the top of the kit body (10).

Then, when the transfer and stirring unit 130 transfers the body fluid analysis kit 1 to the lower side of the connection unit 160 and aligns it at the connection position (P3), the lifting unit of the connection unit 160 lowers and connects the probe terminal 162. is electrically connected to the connection pin 31 of the sensor connector 30 of the body fluid analysis kit 1. Therefore, the electrical signal generated from the biosensor 40 of the body fluid analysis kit 1 is transmitted to the control unit 140 through the probe terminal 162, and the control unit 140 receives the electricity transmitted through the probe terminal 162. Based on the signal, the components of the body fluid are analyzed using a body fluid analysis program.

The information analyzed by the control unit 140 may be output to the outside through the display unit 150 or transmitted to the server of the disease diagnosis system through wireless or wired communication.

While body fluid analysis is in progress by connecting the body fluid analysis kit 1 and the probe terminal 62 at the connection part 160, the kit body loading part 110 and the kit cover loading part 115 are used as described above. Insertion of the kit body (10) and kit cover (20), input of the bodily fluid container (2) into the container handling unit (120) and extraction of bodily fluid, administration of bodily fluid by the bodily fluid administration unit (140), kit assembly unit (150) When the joining operation of the kit body 10 and the kit cover 20 proceeds continuously and the analysis operation at the connection part 160 is completed, the analysis operation for the next body fluid analysis kit 1 continues. You can.

This series of processes is performed continuously and repeatedly, allowing the body fluids of a large number of test subjects to be analyzed in a short period of time.

The body fluid analysis kit 1, on which the body fluid analysis has been completed in the connection unit 160, is removed by the tester by discharging it to the outside of the main body 101 through the kit unloading unit 118.

As described above, the automatic bodily fluid analysis system 100 of the present invention includes a kit for bodily fluid analysis (1), a kit body (10) storing a plurality of reagents (L), and a kit cover provided with a biosensor (40) for analysis. It is separated into (20) and put into the main body (101), and the body fluid is automatically administered to the reagent (L) of the kit main body (10) in a predetermined amount, and then the kit cover (20) is attached to the kit main body (10). and the body fluid analysis kit (1) is assembled. In addition, the sensor connector 30 of the assembled body fluid analysis kit 1 is connected to the probe terminal 162 at the connection portion 160, so that components can be automatically analyzed by signals transmitted from the biosensor 40.

Therefore, the process of administering body fluid to the body fluid analysis kit (1), the assembly of the body fluid analysis kit (1) by combining the kit body (10) and the kit cover (20), the body fluid analysis kit (1) and the probe Since the series of component analysis processes through connection to the terminal 162 can be performed automatically and continuously, not only can body fluid analysis and disease diagnosis be performed very easily and quickly, but also the tester can become infected with body fluids during the analysis process. The possibility of this happening can be almost completely ruled out.

In addition, the analysis function of body fluids can be performed by mixing reagents and body fluids in the body fluid analysis kit (1), thereby further improving the accuracy of body fluid analysis.

In the above, the present invention has been described in detail with reference to embodiments, but those skilled in the art will be able to make various substitutions, additions, and modifications without departing from the technical spirit described above. It is natural, and such modified embodiments should also be understood as falling within the scope of protection of the present invention as defined by the patent claims attached below.

L: Reagent 1: Kit for body fluid analysis
2: Body fluid container 10: Kit body
11: Reagent chamber 12: Rib guide groove
13: Sensor receiving groove 16: Step
17: Hook guide groove 18: Locking jaw
20: Kit cover 22: Sealing rib
24: Outer sealing material 27: Hook portion
30: sensor connector 40: biosensor
41: Sensing unit 100: Automatic body fluid analysis system
101: main body 110: kit body loading part
111: loading plate 115: kit cover loading part
116: Cover seating plate 120: Container handling unit
122: Container transfer unit 125: Container opening and closing unit
126: Container unloading unit 130: Transfer and stirring unit
131: fixed unit 132: movable unit
140: Body fluid injection unit 141: Multi-joint manipulator
142: Syringe pump unit 143: Pipette
144: pipette standby unit 145: pipette disposal unit
150: Kit assembly part 151: Cover transfer unit
152: Cover gripper 160: Connection part
161: Probe holder 162: Probe terminal
164: Elevating rail 170: Control unit
190: Display panel

Claims (7)

A kit body in which a plurality of reagent chambers in which reagents are stored are formed to be open upward, a kit cover coupled to the upper part of the kit body after a certain amount of the test subject's body fluid is administered to the reagent in the reagent chamber, and the kit cover A body fluid including a plurality of sensor connectors installed to correspond to the reagent chamber and a plurality of biosensors that are coupled to each of the sensor connectors and inserted into the reagent chamber when the kit cover is coupled to the upper part of the kit body and immersed in the reagent. Analysis kit;
A loading plate on which the kit body of the body fluid analysis kit is seated when the kit body and the kit cover are separated;
a body fluid administration unit that administers a certain amount of body fluid of the test subject to the reagent chamber of the kit body on the loading plate; and,
The loading plate is positioned from a loading position where the kit body is seated on the loading plate to a bodily fluid administration position where the body fluid is administered by the body fluid administration unit, a kit assembly position where the kit cover of the body fluid analysis kit is coupled to the upper part of the kit body, and The body fluid analysis kit is electrically connected to the probe terminal of the connection part and continuously transferred to the connection position where body fluid analysis is performed, and the loading plate is installed between the body fluid administration position and the kit assembly position, or between the kit assembly position and the connection position. A transfer and stirring unit that agitates the reagents and body fluids in the reagent chamber by repeatedly moving back and forth at a certain distance and a certain number of times to vibrate the kit body on the loading plate;
Including,
The transfer and stirring unit consists of a fixed unit installed to extend to the loading position, the body fluid administration position, the kit assembly position, and the connection position, and a movable unit movably installed on the fixed unit and coupled to the loading plate at the upper end. A body fluid and reagent mixing device for an automatic body fluid analysis system, including a linear motion module, wherein the movable unit vibrates the kit body on a loading plate by horizontally reciprocating with respect to the fixed unit. delete The bodily fluid and reagent mixing device of the automatic bodily fluid analysis system according to claim 1, wherein the kit guide groove supported by the lower end of the kit body is inserted into the upper surface of the loading plate and is formed concave downward. The method of claim 1, wherein the body fluid administration unit includes a multi-joint manipulator configured to be movable in any direction, and is installed on the multi-joint manipulator to suck the body fluid from the body fluid container and extract the body fluid from the upper side of the reagent chamber of the kit body. A syringe pump unit that generates negative pressure and positive pressure for discharge, a pipette standby unit in which a plurality of unused pipettes are detachably installed at the tip of the syringe pump unit, and the syringe pump A body fluid and reagent mixing device for an automatic body fluid analysis system that includes a pipette disposal unit that is coupled to the unit and collects pipettes that are separated from the syringe pump unit after use. The method of claim 1, wherein before the kit body is placed on the loading plate, the kit cover is detachably coupled to the lower part of the kit body and transported, and when the kit body is placed on the loading plate, the kit cover is attached to the kit body. After being separated from the lower part of the body, it is turned over and put into the automatic bodily fluid analysis system,
With the kit cover coupled to the lower part of the kit body, the upper part of the reagent chamber of the kit body is sealed by a temporary sealing material in the form of a film that is detachably attached to the upper surface of the kit body,
The kit body is made in the form of a box with an open lower surface, and a sensor receiving groove into which the biosensor is inserted is formed on the outer side of the reagent chamber when the kit cover is coupled to the lower part of the kit body. Body fluids and reagents of the automatic body fluid analysis system Mixing device. The method of claim 5, wherein when the kit cover is coupled to the upper part of the kit body at the kit assembly position and the biosensor is inserted into the reagent chamber, the biosensor is disposed in a position eccentric in one direction from the central axis of the reagent chamber. Body fluid and reagent mixing device for automatic body fluid analysis system. The method of claim 1, wherein the kit for bodily fluid analysis further includes a locking unit that restrains the kit cover to the kit body so that the kit cover is not separated from the kit body after the kit cover is coupled to the upper part of the kit body at the kit assembly position. A bodily fluid and reagent mixing device for an automatic bodily fluid analysis system.