KR102545535B1 - A composition for anti-bacterial, skin-improving or anti-allergic activity containing extracts of Cudrania tricuspidata seed - Google Patents

Abstract

The present invention relates to an antibacterial composition containing a kkujippong seed extract as an active ingredient, and more particularly, to an antibacterial, skin improving or anti-allergic cosmetic composition, a food composition, and a pharmaceutical composition containing the kkujippong seed extract as an active ingredient. will be.

Description

A composition for anti-bacterial, skin-improving or anti-allergic activity containing extracts of Cudrania tricuspidata seed}

The present invention relates to an antibacterial composition containing a kkujippong seed extract, and more particularly, to a cosmetic composition, a food composition, and a pharmaceutical composition having antibacterial, skin-improving or anti-allergic effects.

It is known that 73 genera and 1,000 species of Moraceae, to which Cudrania belongs, are distributed worldwide, and it has been reported that 10 species of 5 genera inhabit Korea. Among them, three species of Cudrania mulberry were reported to grow wild in India, China, and Australia, respectively, and two species in Japan.

The Cudrania tricuspidata bureau, which grows wild in the mountains of the southern region of Korea, is a deciduous broad-leaved small arboreous tree belonging to the Moraceae family. It is hardy and has a characteristic of producing red fruits around October. It is used for food (leaves, fruits), medicine (stems), civil engineering materials, and feed for silkworms (leaves). In addition, when the leaves of the Cudrania mulberry tree are fed to silkworms, the threads are pulled out to make strings such as geomungo or gayageum. In addition, it is used for landscaping, textile (leaves), and grafting of Sanghwang mushroom spawn.

In addition, it is resistant to pests and diseases, making it a good income crop in agriculture and forestry. Currently, although the stem and root of the Cudrania mulberry tree are widely used in the private sector for cancer treatment, the use of the stem and root as a food raw material is limited, so only the fruit and leaves can be used for food. As a supplementary material for baking, the fruit is used to make edible jam or to make alcoholic beverages. In particular, compared to green tea leaves and mulberry leaves, kkujippong leaves have a higher content of protein, dietary fiber, vitamin B1, vitamin B2, calcium, potassium, GABA (γ-amino byturic acid), and rutin. Therefore, a number of green tea substitutes and extracts are being developed. However, since there are many thorns, it takes a lot of labor and time to collect leaves and fruits at farmhouses, so improved varieties without thorns are in progress. It's a reality.

Research on materials showing cytotoxicity to HepG3, a liver cancer cell, as a search for anti-cancer active substances of Cudrania quinoa, and isoprenylated xanthone or isoprenylate pravanone, which show inhibitory activity in cancer cell lines of the digestive system from root skin of Cudrania quince. (isoprenylated flavanones) It is known for its cancer-fighting efficacy through research on isolating substances belonging to the system, and in addition, it has blood pressure lowering action, antidepressant action, Parkinson's disease prevention, hyperlipidemia and arteriosclerosis inhibition, pain relief, diabetes prevention, menstrual pain, cold sensitivity relief. It is known to have anti-inflammatory properties.

Korean Patent Registration No. 10-1053177 'Health functional food' in relation to the physiological activity of Cudrania mulberry tree discloses a health functional food having blood sugar lowering activity using leaves of Cudrania mulberry tree, elm tree, Wasp pine, etc. There is little information on the antibacterial, skin-improving, or anti-allergic activity of seeds containing them as active ingredients.

As the discovery and preservation of domestic genetic resources has been strengthened in accordance with the recent international trend such as the fermentation of the Nagoya Protocol, the necessity of research for the export of processed products by deriving the efficacy of differentiated native kkujippong from China is emerging.

Republic of Korea Patent No. 10-1053177 (2011.07.26)

One object of the present invention is to provide an antibacterial composition containing kkujippong seed extract as an active ingredient.

Another object of the present invention is to provide a cosmetic composition for antibacterial, skin improvement or anti-allergy containing kkujippong seed extract as an active ingredient.

Another object of the present invention is to provide a food composition for antibacterial, skin improvement or anti-allergy containing kkujippong seed extract as an active ingredient.

Another object of the present invention is to provide an antibacterial or anti-allergic pharmaceutical composition containing kkujippong seed extract as an active ingredient.

The present invention relates to an antibacterial composition containing an extract of kujippong seeds as an active ingredient, preferably, the extract of kujippong seeds is characterized in that a lower alcohol is extracted as a solvent, more preferably, the lower alcohol is 80% ethanol characterized by

In addition, the antibacterial effect of the composition is against one or more microorganisms selected from the group consisting of Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus mutans It is achieved by growth inhibitory activity.

Another embodiment of the present invention relates to a cosmetic composition for antibacterial, skin improvement or anti-allergy, and more specifically, the cosmetic composition is characterized by whitening or skin aging improvement efficacy.

On the other hand, another embodiment of the present invention relates to a food composition for antibacterial, skin improvement or anti-allergy.

In addition, another embodiment of the present invention relates to a pharmaceutical composition for antibacterial or antiallergic use, wherein the pharmaceutical composition includes IgE concentration contained in serum, Ovalbumin-specific IgE concentration contained in serum, and IL contained in serum. It is characterized by reducing the concentration of -4 cytokines. More specifically, the anti-allergic agent relates to a pharmaceutical composition for chronic asthma.

According to the antibacterial composition containing the kkujippong seed extract according to the present invention, it has antimicrobial activity by inhibiting the growth of microbial strains and helps improve skin through collagenase inhibitory activity, tyrosinase inhibition or elastase inhibitory effect, serum Provides a cosmetic composition, food composition and pharmaceutical composition having antibacterial, skin improving and anti-allergic effects by reducing the concentration of IgE contained in serum, the concentration of ovalbumin-specific IgE contained in serum, or the concentration of IL-4 cytokine contained in serum can do.

Figure 1 shows the results of measuring the electron donating (DPPH) activity of the kkujippong fruit and seed extracts extracted by solvent.
Figure 2 shows the results of measuring the phenol content of kkujippong fruit and seed extracts extracted by solvent.
Figure 3 shows the results of measuring the flavonoid content of kkujippong fruit and seed extracts extracted by solvent.
Figure 4 shows the results of measuring the collagenase inhibitory activity of kkujippong fruit and seed extracts extracted by solvent.
Figure 5 shows the results of measuring the elastase inhibitory activity of the kkujippong fruit and seed extracts extracted by solvent.
Figure 6 shows the results of measuring the tyrosinase inhibitory activity of kkujippong fruit and seed extracts extracted by solvent.
Figure 7 shows the effect of suppressing asthma-induced mouse serum IgE concentration of kkujippong fruit and seed extracts extracted by solvent.
Figure 8 shows the effect of suppressing the concentration of ovalbumin-specific IgE in asthma-induced mouse serum of kkujippong fruit and seed extracts extracted by solvent.
Figure 9 shows the inhibitory effect of IL-4 cytokine concentration in serum of asthma-induced mice of kkujippong fruit and seed extracts extracted by solvent.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and should be understood to include all modifications, equivalents or substitutes included in the spirit and technical scope of the present invention.

Terms used in this application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the application, terms such as "comprise" or "have" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features or It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

The present invention extracts kkujippong leaves, fruits, and seeds for each solvent to measure microbial strain inhibitory activity, measure electron donating (DPPH) activity, measure phenol content, measure flavonoid content, measure collagenase inhibitory activity, measure elastase inhibitory activity , Measurement of tyrosinase inhibitory activity, IgE concentration of asthma-induced mouse serum, ovalbumin-specific IgE concentration of asthma-induced mouse serum, and IL-4 cytokine concentration contained in serum were measured. Provides an antibacterial composition containing as an active ingredient, preferably, the kkujippong seed extract is extracted with a lower alcohol as a solvent, more preferably, the kkujippong seed extract is extracted with 80% ethanol as a solvent. It's about the composition.

In addition, the present invention inhibits the growth of microorganisms as an antimicrobial effect activity, preferably Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus mutans (Streptococcus mutans). It relates to an antibacterial composition that is achieved by growth inhibitory activity against one or more microorganisms selected from the group consisting of.

In addition, the present invention provides a cosmetic composition for antibacterial, skin improvement or anti-allergy containing kkujippong seed extract as an active ingredient, and more preferably provides a cosmetic composition having a whitening or skin aging improvement effect. The cosmetic composition may be prepared in any form conventionally prepared in the art, and examples include, but are not limited to, lotion, toner, lotion, nutrient lotion, nutrient cream, massage cream, essence, pack, etc. .

In addition, the present invention provides a food composition for antibacterial, skin improvement or anti-allergy containing kkujippong seed extract as an active ingredient. The food composition may further include a food-acceptable food auxiliary additive in addition to the Kkujippong seed extract, and may be appropriately used according to conventional methods, and the mixed amount of the active ingredient is the purpose of its use, for example, preventive health or It can be suitably determined according to the therapeutic treatment.

Foods to which the kkujippong seed extract of the present invention can be added include, for example, various foods, beverages, gum, jam, tea, vitamin complexes, and health functional foods.

In addition, the present invention provides a pharmaceutical composition for antibacterial or anti-allergic containing kkujippong seed extract as an active ingredient. More specifically, the pharmaceutical composition is characterized by reducing the concentration of IgE in serum, the concentration of ovalbumin-specific IgE in serum, or the concentration of IL-4 cytokine in serum, which has been identified as one of the causes of allergy. More preferably, the anti-allergy is characterized by chronic asthma. The pharmaceutical composition of the present invention may include only the crude drug extract itself, but may further include suitable carriers, excipients, and diluents commonly used in the preparation of pharmaceutical compositions, and pharmaceutically acceptable formulations according to conventional methods. , For example, it can be prepared in dosage forms such as powder, tablet, granule, capsule or injection.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for exemplifying the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example One. kkujippong Preparation of Seed Extract

After harvesting, drying, cutting, and extracting 80% ethanol at the boiling point for 2 hours, it is filtered by darkening with Whatman (No.1) filter paper, and then rotated. Samples were prepared by drying (PVTFA10AT, ILSIN, Suwon, Korea) the viscous extract obtained by concentration under reduced pressure with an evaporator in a lyophilizer.

Experimental example 1. Antibacterial activity test

The strains used in the antibacterial activity test are listed in Table 1 below, and each strain is activated by repeating the cultivation of dormant bacteria three times according to each bacteria growth condition after being distributed from the Korea Gene Bank (KCTC) and ATCC, respectively. made it The medium was used by Difco (Difco Laboratories, Detroit, MI, USA). The antibacterial activity of the fruit and seed extracts used in the experiment was verified by paper disc diffusion. Each bacterium was inoculated by obtaining 2-3 colonies (10 ^4-5 CFU/mL). The extract was dissolved in DW at 100 ug/ul, and 50 ul each was dropped on a paper disc (diameter 8 mm). After the paper disk was completely dried, it was placed on a flat medium and maintained at 35 ° C. After 20-24 hours, the clear zone, a growth inhibition zone around the paper disk, was measured to investigate the antibacterial activity.

strain name
Gram-negative bacteria, Gram(-) Salmonella typhimurium ATCC 13311 Escherichia coli KCTC 1039
Gram-positive bacteria, Gram(+)
Staphylococcus aureus KCTC 1621 Staphylococcus epidermis KCTC 1917 Streptococcus mutans KCTC 5316

The results of the antibacterial activity test of the fruit and seed extract of Kujippong are shown in Table 2 below. The CTFW kkujippong fruit described in Table 2 below is an extract extracted with water, CTFE is an extract obtained by extracting the kkujippong fruit with 80% ethanol, CTSW is an extract obtained by extracting the kkujippong seeds with water, and CTSE is an extract obtained by extracting the kkujippong seeds with 80% ethanol. indicate

antibacterial activity CTFW CTFE CTSW CTSE Salmonella typhimurium 0 0 0 0 Escherichia coli 0 0 0 0 Staphylococcus aureus 0 0 0 13.7 Staphylococcus epidermis 0 0 0 10.7 Streptococcus mutans 0 0 0 13.0

It was found to inhibit Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus mutans strains in 80% ethanol extract (CTSE) of Cudrania seeds.

Experimental example 2. Electronic donation ( DPPH ) active measurement

The electron donating ability was measured according to the method of Blois. That is, 0.8 mL of a diluted DPPH solution of 0.2 mM was added to 0.2 mL of the sample solution extracted from the kkujippong fruits and seeds by solvent, stirred, allowed to stand for 30 minutes, and then absorbance was measured at 517 nm. The electron donating ability is expressed as a percentage of the difference in absorbance between the added group and the non-added group of the sample solution. Figure 1 shows the results of measuring the electron donating activity of kkujippong fruit and seed extracts extracted by solvent. According to Figure 1, it was shown that the higher the concentration of the kkujippong fruit and seed extract, the higher the electron donating radical (DPPH radical) scavenging activity.

Experimental example 3. Determination of phenol content

1 mL of the sample extracted from the fruits and seeds of Cudrania japonica by solvent was mixed with 1 mL of 10% Na ₂ CO ₃ , 1 mL of 2 N Folin-denis reagent was added, and the absorbance was measured at 750 nm after standing for 30 minutes. For the total phenolic compound content, a calibration curve was prepared using caffeic acid, and the total phenolic compound content was converted using this curve. Figure 2 shows the results of measuring the phenol content of kkujippong fruit and seed extracts extracted by solvent. According to Figure 2, at an extract concentration of 6.25mg / ml, kkujippong fruit water extraction (CTFW) 2.35ug / ml, kkujippong fruit 80% ethanol extraction (CTFE) 2.22ug / ml, kkujippong seed water extraction (CTSW) 2.44ug / ml , Cudrania seeds 80% ethanol extraction (CTSE) was 3.53ug/ml, and it was found that 80% ethanol extraction of Cudrania seeds showed a high phenol content.

Experimental example 4. Determination of flavonoid content

Add 500 μL of diethylene glycol to 200 μL of the same sample as in the total phenol analysis, mix with 50 μL of 1 N NaOH, vortex, shake in a constant temperature water bath at 37 ° C for 1 hour, and use an ELISA reader ((ELISA reader) to measure the absorbance at 420 nm. At this time, using naringin as a standard material, the analysis is repeated three times. Figure 3 shows the results of measuring the flavonoid content of the fruit and seed extract of kkujippong extracted by solvent. According to Figure 3, kkujippong fruit water extraction (CTFW) 1.96ug / ml, kkujippong fruit 80% ethanol extraction (CTFE) 1.99ug / ml, kkujippong seed water extraction (CTSW) 2.06ug / ml at an extract concentration of 6.25mg / ml , 80% ethanol extraction (CTSE) of Cudrania seeds was 2.74ug/ml, and the amount of total flavonoids was also the highest in total phenol content.

Experimental example 5. collagenase Inhibition activity measurement

In the reaction zone, 4 mM calcium chloride (CaCl _{2 )} was added to 0.1 M Tris-HCI buffer (pH 7.5), and 4-phenylazobenzyloxycarbonyl-Pre-Leu-Gly-Pro-Arg (4-phenylazobenzyloxycarbonyl-Pro-Leu 0.15 ml of collagenase (0.2 mg/ml) was added to a mixture of 0.25 ml of substrate liquid and 0.1 ml of sample solution in which Gly-Pro-Arg, 0.3 mg/ml) was dissolved, and left at room temperature for 20 minutes, followed by 6% citric After stopping the reaction by adding 0.5 ml of acid, 1.5 ml of ethyl acetate was added to measure the absorbance of the supernatant at 320 nm. Collagenase inhibitory activity is expressed as the rate of decrease in absorbance between the sample solution added and unadded. Figure 4 shows the results of measuring the collagenase inhibitory activity of the kkujippong fruit and seed extracts extracted by solvent. As a result of measuring the inhibitory activity of collagenase in cudrania fruit and seed extracts, at an extract concentration of 12.5 mg / ml, water extraction of cudra quince fruit (CTFW), cudra quince fruit 80% ethanol extraction (CTFE), and cudra quince seed water extraction (CTSW) ), the inhibitory activity was less than 10%, but the 80% ethanol extraction (CTSE) of kkujippong seeds showed a high collagenase inhibitory activity of 79%.

Experimental example 6. Measurement of elastase inhibitory activity

Using N-succinyl-(L-Ala)3-p-nitroanilide as substrate, p-nitroanilide at 37°C for 30 min. By measuring the amount of (p-nitroanilide) produced, porcine pancreas elastase inhibitory activity is investigated. Each test solution was prepared to a certain concentration, and 0.1 ml each was taken in a test tube. Elastase dissolved in 50 mM Tris-HCl buffer (pH 8.6), pancreatic solution (Type I: From N-succinyl-(L-Ala)3-p-nitroy dissolved in 50 mM Tris-HCl buffer (pH 8.6) as a substrate after adding 0.05 ml of Porcine Pancreas (0.1 units/ml)) solution. 0.1 ml of nilide (N-succinyl-(L-Ala)3-p-nitroanilide, 0.1 mg/ml) was added, reacted for 30 minutes, and absorbance was measured at 410 nm using a microplate reader. The elastase inhibitory activity is expressed as the rate of decrease in absorbance between the added and non-added groups of the sample solution. Figure 5 shows the results of measuring the elastase inhibitory activity of the kkujippong fruit and seed extracts extracted by solvent. As a result of measuring the inhibitory activity of elastase in the fruit and seed extracts of Cudrania cudrania, at a concentration of 6.25 mg/ml, 49.3% of Cudrania quince fruit extract (CTFW), 58.7% of cudrania quince fruit 80% ethanol extraction (CTFE), 58.7% of Cudrania culata seed water extraction (CTSW) 64.9%, 80% ethanol extraction (CTSE) 78.0% of Cudrania seeds, 80% ethanol extraction showed high elastase inhibitory activity.

Experimental example 7. Measurement of tyrosinase inhibitory activity

110 Unit/ml mushroom tyrosinase was added to a mixture containing 0.2 ml of substrate solution and 0.1 ml of sample solution in which 10 mM L-DOPA was dissolved in 0.5 ml of 1/15M sodium phosphate buffer at pH 6.8 in a test tube. ) 0.2 ml was added, reacted at 25° C. for 2 minutes, and the absorbance of DOPA chrome generated in the reaction solution was measured at 475 nm using a microplate reader. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added group and non-added group. Figure 6 shows the results of measuring the tyrosinase inhibitory activity of kkujippong fruit and seed extracts extracted by solvent. As a result of measuring the inhibitory activity of tyrosinase in cudrania fruit and seed extracts, at a concentration of 12.5 mg/ml, 2.4% of cudrania fruit extract (CTFW), 13.1% of cudrania quince fruit 80% ethanol extraction (CTFE), 13.1% of cudrania quince seed extract (CTSW) 28.3%, 80% ethanol extraction (CTSE) 60.7%, and 80% ethanol extraction of Cudrania seeds showed high tyrosinase inhibitory activity.

Example 2. kkujippong Animal model production to confirm anti-allergic efficacy of extract

Sensitize first by intraperitoneally administering 0.2 mL of a solution containing 0.2% of ovalbumin (chicken egg albumin, grade V) mixed with aluminum hydroxide gel [Al(OH)3 gel in a 1:1 ratio. 14 days after intraperitoneal administration, re-sensitize in the same way as the first sensitization. First intraperitoneal administration After inhalation of 0.2% OVA for 1 hour using a nebulizer every day for 10 days from the 21st to the 30th, the test substance was orally administered as shown in Table 3 below. Anesthetize with ether 12 hours after the last OVA inhalation. Open the stomach and draw blood from the vena cava. The collected blood is stored at 4℃ for 2 to 4 hours, and then centrifuged (1,200 g, 15 minutes) to separate serum. After blood is drawn, a tracheotomy is performed and a cut-down tube is inserted through the tracheostomy window to collect bronchoalveolar lavage fluid (BALF).

Test group notation test group test substance dose Administration method NOR Normal control group (NOR) 1xPBS PO CTR Negative Control (CTR) 1xPBS PO Dexa3 positive control Dexamethasone 3 mg/kg PO CTSW200 Administration group 1 Cudrania Seed Water Extraction 200 mg/kg PO CTSW500 Administration group 2 Cudrania Seed Water Extraction 500 mg/kg PO CTSE200 Administration group 3 Cudrania seeds 80% ethanol extraction 200 mg/kg PO CTSE500 Administration group 4 Cudrania seeds 80% ethanol extraction 500 mg/kg PO CTLW:CTFW=4:1(200) Administration group 5 Cudrania leaf water extraction + Cudrania fruit water extraction = 4:1 200 mg/kg PO CTLW:CTFW=4:1(500) Administration group 6 Cudrania leaf water extraction + Cudrania fruit water extraction = 4:1 500 mg/kg PO

Experimental example 8. Asthma-induced mouse serum IgE Concentration suppression effect

The amount of total IgE in the serum isolated in Example 2 was quantified with an ELISA kit (Mouse IgE ELISA Set Cat. No. 555248, BD). After diluting the antibody in buffer solution and coating on 96-well, it is left at 4℃ for 20 hours. Wash each well 3 times and dispense 100 μL of serum (2-fold dilution). After being left at room temperature for 2 hours, washed 5 times, treated with 100 μL of antibody Avidin-HRP conjugated, left at room temperature for 1 hour, washed again, and then dispensed with 100 μL of TMB substrate and left in the dark for 30 minutes. After processing 50 μL of the solution, measure the absorbance at 450 nm with an ELISA reader. Figure 7 shows the effect of suppressing asthma-induced mouse serum IgE concentration of kkujippong fruit and seed extracts extracted by solvent. When 80% ethanol extract 200 (CTSW200) of kkujippong seeds was administered to asthma model animals, the concentration of IgE in the blood decreased the most.

Experimental example 9. Ovalbumin specificity of asthmatic mouse serum IgE concentration inhibitory effect

ELISA was performed to measure the concentration of ovalbumin-specific IgE in the serum isolated in Example 2. As an antigen, OVA is diluted in coating buffer at a concentration of 20ug/mL, coated on 96-well, and left at 4℃ for 20 hours. After washing each well with buffer solution three times, dispense 100 μL of serum (2-fold dilution). After being left at room temperature for 2 hours, washed with a buffer solution 5 times, treated with 100 μL of antibody Avidin-HRP conjugated, left at room temperature for 1 hour, and washed again. Here, 100 μL of TMB substrate is dispensed, left in the dark for 30 minutes, treated with 50 μL of the solution, and absorbance is measured at 450 nm using an ELISA reader. Figure 8 shows the effect of suppressing the concentration of ovalbumin-specific IgE in asthma-induced mouse serum of kkujippong fruit and seed extracts extracted by solvent. When 80% ethanol extract 200 (CTSW200) of kkujippong seeds was administered to asthma model animals, the concentration of ovalbumin-specific IgE in the blood decreased the most.

Experimental example 10. IL- in Asthmatic Mouse Serum 4 Cytokines (IL-4 cytokines ) concentration suppression effect

The amount of IL-4 cytokine (IL-4 cyotkine) concentration in the serum isolated through Example 2 was measured using ELISA kit (BD). Figure 9 shows the inhibitory effect of IL-4 cytokine (IL-4 cyotkine) concentration in serum of asthma-induced mice of kkujippong fruit and seed extracts extracted by solvent. Of the animal test drugs, it can be seen that the concentration of IL-4cytokine in the blood was significantly reduced in both 200 (CTSE200) and 500 (CTSE500) extracted from 80% ethanol of Kujippong seeds.

Claims (10)

An antimicrobial composition against one or more microorganisms selected from the group consisting of Staphylococcus epidermidis and Streptococcus mutans containing 80% ethanol extract of kkujippong seeds as an active ingredient. delete delete delete An antimicrobial cosmetic composition for at least one microorganism selected from the group consisting of Staphylococcus epidermidis and Streptococcus mutans containing 80% ethanol extract of Cudrania seeds as an active ingredient. A cosmetic composition for preventing or improving skin wrinkles containing an ethanol extract of kkujippong seeds as an active ingredient. A food composition comprising an antibacterial composition against one or more microorganisms selected from the group consisting of Staphylococcus epidermidis and Streptococcus mutans containing 80% ethanol extract of Cudrania seeds as an active ingredient . An antibacterial pharmaceutical composition for one or more microorganisms selected from the group consisting of Staphylococcus epidermidis and Streptococcus mutans containing 80% ethanol extract of kkujippong seeds as an active ingredient. delete A food composition comprising a composition for preventing or improving skin wrinkles containing an ethanol extract of kkujippong seeds as an active ingredient.

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