KR102523775B1 - Food composition and health functional food containing Lactobacillus plantarum IDCC 3501 with proteolytic ability - Google Patents

Abstract

The present invention relates to a food composition and health functional food having protein degrading ability, and includes Lactobacillus plantarum IDCC 3501 [Accession Number: ATCC BAA-2838].

Description

Food composition and health functional food containing Lactobacillus plantarum IDCC 3501 with proteolytic ability}

The present invention relates to a food composition and health functional food containing Lactobacillus plantarum IDCC 3501 having protein degrading ability.

Protein constitutes 11-14% of average energy intake and is used in all metabolic processes in the body. Dietary protein is obtained from animal and plant raw materials, and in order for the ingested protein to be used in the body, it must be broken down into amino acids through peptides.

Protein degradation involves proteases that hydrolyze proteins to break peptide bonds. Recently, efforts have been made to increase the absorption rate in the body by increasing the concentration of amino acids in blood through protein decomposition as well as balanced intake of animal and vegetable proteins.

Korean Patent Registration No. 10-1280855 (Announced on July 02, 2013)

An object of the present invention is to provide a food composition and health functional food containing Lactobacillus plantarum IDCC 3501 having a protein degrading ability including lactic acid bacteria.

The object of the present invention is achieved by including Lactobacillus plantarum IDCC 3501 [Accession Number: ATCC BAA-2838] in a food composition having protein degrading ability.

The food composition may include 0.01% by weight to 50% by weight of the Lactobacillus plantarum IDCC 3501.

The object of the present invention is achieved by including Lactobacillus plantarum IDCC 3501 [Accession Number: ATCC BAA-2838] as an active ingredient in a health functional food having protein decomposition ability.

The health functional food may include 0.1% to 100% by weight of the Lactobacillus plantarum IDCC 3501.

The present invention relates to Lactobacillus plantarum IDCC 3501 [Accession Number: ATCC BAA-2838] having proteolytic ability.

According to the present invention, a food composition and health functional food containing Lactobacillus plantarum IDCC 3501 having protein degrading ability are provided.

Figure 1 shows the clear zone shown by protease activity when each strain was cultured in pea protein and skim milk medium,
Figure 2 is a graph comparing protease activity when each strain is cultured in pea protein and skim milk medium.

The present invention will be described in more detail with reference to the following drawings.

Since the accompanying drawings are only examples shown to explain the technical spirit of the present invention in more detail, the spirit of the present invention is not limited to the accompanying drawings. In addition, in the accompanying drawings, sizes and intervals may be exaggerated unlike actual ones in order to explain the relationship between each component.

Probiotics are live microorganisms that, when ingested in sufficient quantities, provide beneficial health effects to the host, influencing nutrient absorption and utilization. Probiotics can induce protease and peptidase activity, which are proteolytic enzymes, and release enzymes involved in protein digestion. In addition, probiotics can enhance the absorption and transport of peptides and amino acids by increasing the absorption and transport capacity of the colonic epithelium.

The present invention relates to a strain having excellent protein degradation ability among strains of the genus Lactoacillus and Lactococcus.

Lactobacillus plantarum IDCC 3501 [accession number: ATCC BAA-2838] of the present invention has excellent protease activity.

Lactobacillus plantarum IDCC 3501 of the present invention may be in the form of a culture.

Lactobacillus plantarum IDCC 3501 of the present invention can be used in probiotics preparations that help protein degradation.

Lactobacillus plantarum IDCC 3501 of the present invention has proteolytic ability to help protein digestion and amino acid absorption, and in particular, it can help amino acid absorption of those who have difficulty digesting protein.

The present invention relates to Lactobacillus plantarum IDCC 3501 [accession number: ATCC BAA-2838] having protein degrading ability, a food composition containing the same, and health functional food containing the same.

Lactobacillus plantarum IDCC 3501 can be in the form of live cells or lysates.

The food composition or health functional food of the present invention includes compositions for all types of food, such as functional food, nutritional supplement, health food, and food additives. The above type of food composition or health functional food can be prepared in various forms according to conventional methods known in the art. For example, as a health food, it can be prepared in the form of gum, vitamin complex, tea, juice and drink containing lactic acid bacteria, and a food composition containing Lactobacillus plantarum IDCC 3501 as an active ingredient can be granulated, encapsulated or powdered. It can be digested and consumed.

The food composition or health functional food of the present invention may include components commonly added during food preparation, and may include, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, jujube extract, licorice extract, and the like may be further included.

Food compositions or health functional foods are various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its It may contain salts, organic acids, protective colloidal thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, it may contain extracts for the production of natural fruit juice, fruit juice beverages and vegetable beverages. These components may be used independently or in combination.

As another example, the formulation of the food composition or health functional food may be in the form of a powder, granule, pill, tablet, or capsule as well as a general food or beverage form.

The type of food is not particularly limited, and examples of food include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, There are drinks, alcoholic beverages, vitamin complexes, and the like, and may include all foods in a conventional sense.

The content of Plantarum IDCC 3501 in the food composition may be 0.001 to 50% by weight, 0.01 to 50% by weight, 0.1 to 50% by weight, 0.1 to 20% by weight or 1 to 50% by weight.

In the health functional food, the content of Plantarum IDCC 3501 may be 0.1 to 100% by weight, 0.1 to 50% by weight, 0.1 to 30% by weight, 0.1 to 10% by weight or 1 to 10% by weight.

In general, when preparing food or beverage, the content of Lactobacillus plantarum IDCC 3501 may be 0.1 to 50% by weight, 0.1 to 20% by weight, or 0.1 to 10% by weight. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be less than the above range.

Among the food compositions according to one embodiment, the beverage may contain various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. The aforementioned natural carbohydrates may be monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the sweetener, natural sweeteners such as thaumatin and stevia extract, or synthetic sweeteners such as saccharin and aspartame may be used.

In addition to the above, the food composition or health functional food according to one embodiment includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, and stabilizers. , preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages. In addition, the food composition or health functional food of the present invention may contain fruit flesh for the production of natural fruit juice, fruit juice beverage and vegetable beverage. These components may be used independently or in combination.

Specific examples of food compositions or health functional foods are as follows.

Mineral nutritional additives, mineral supplements, mineral formulations, fruit flavored dietary supplement drink mix in powder form, nutritional supplement drink mix in powder form, vitamin and mineral supplements, vitamins and minerals, vitamins and vitamins, vitamin formulations, vitamins / minerals / amino acids and trace element dietary supplements, vitamin A agents, vitamin B agents, vitamin C agents, vitamin D agents, vitamin supplements, vitamin tablets, vitamins, drugs for bone disease treatment, powder for meal replacement, nutritional supplements for dietary supplements, beverages for dietary supplements, diet Supplementary beverages, antioxidants for dietary supplements, dietary fiber, dietary starch, dietary supplements and nutritional additives, vitamins in the form of food supplements, zinc supplement tablets, nutritional supplements with zinc as the main component, liquid vitamin supplements, liquid herbal supplements, Medicinal candy, medicinal candy, pharmaceutical menthol, nutritional supplement bar for energy enhancement, drink mix for medical meal replacement, drink mix for medical meal replacement and dietary supplement, shake for medical meal replacement, snack for medical meal replacement, dietary supplement for medical meal replacement Drink mix, nutritional drink mix for medical meal replacement, medical meal replacement drink, medical meal replacement bar, medical meal replacement powder, medical diet beverage, medical diet beverage preparation, medical dietary supplement, medical dietary therapy product, medical amino acid , medical amino acid preparation, medical candy, medical herb spray, medical herbal medicine, medical herb extract, iron-based nutritional supplement, weight loss medical preparation, weight loss capsule, weight loss medicine, colostrum supplement, calcium supplement, calcium Nutritional supplements, hypoglycemic agents, egg-based health functional foods, protein dietary supplements, food for diabetics, drink mix dietary supplements, lecithin dietary supplements, royal jelly dietary supplements, magnesium-based dietary supplements Dietary supplements, malt dietary supplements, mineral supplements, mineral dietary supplements, mineral food supplements, mineral-based health food supplements, micronutrient dietary supplements, micronutrient food supplements, dietary supplements with cosmetic effects, wheat dietary supplements, Supplement food, vitamin and mineral dietary supplement, vitamin and mineral food supplement, vitamin dietary supplement, vitamin-based health food supplement, dietary supplement, health functional food for dietary supplement, amino acid dietary supplement, amino acid food supplement, amino acid/mineral /Micronutrient dietary supplement, amino acid/mineral/micronutrient food supplement, iron-based dietary supplement, colostrum dietary supplement, therapeutic health functional food, therapeutic or medical health functional food, casein dietary supplement, calcium as the main ingredient Dietary supplements for cholesterol control, soy protein dietary supplements, health food supplements for special diets, glucose dietary supplements, propolis dietary supplements, antioxidant food supplements, dietary supplements for patients, enzyme dietary supplements

Processed grain food, prepared grain product, processed grain food, rice-based snack food, sugar-free candy, candy (candy), edible candy, rice snack, yokan, ginseng snack, ginseng candies, ginseng jellied snacks, ginseng candy , Red ginseng snacks, red ginseng gum, red ginseng yokan, red ginseng tablets, red ginseng jelly snacks, red ginseng ginseng, red ginseng chocolate, red ginseng candy, red ginseng tea

Health functional food with mixed sheep's milk as the main ingredient, processed meat, processed meat, bouillon, bouillon preparation, broth, black pudding [blood sausage], sausage, edible bone marrow, edible protein, edible swallow Nests, edible gelatin, meat products, meat organs, cooked canned meat, canned meat [canned meat], ham, hamburger meat, poultry {non-live}, wildfowl {non-live} , milk beverages containing peanuts, drinking yogurt, mold-aged cheese, fermented milk, powdered milk (except for infants), products containing fat for spreading on bread slices, sour, goat milk, fresh cream, aged cheese, edible casein, fresh unripe Cheese, milk beverages containing almonds, sheep's milk, sheep's cheese, condensed milk, soft cheese, soft white cheese, yogurt, yogurt beverages, milk, milk protein, milk beverages containing milk as the main ingredient, milk cream, milk cream powder, milk (乳) Processed food, lactobacillus beverage, lactobacillus beverage, whey, dairy product, curdled milk, cheese, cheese spread, cheese powder, kefir (milk beverage), condensed milk, kumis (milk beverage), Strained cheese, white cheese, whipped cream, butter, margarine, bone oil, animal fats and oils, shortening, hydrogenated edible oil, edible whale oil [edible], edible bone oil, edible animal oil, edible fish oil ), edible fat, edible beef tallow, non-live fish, non-live fish and shellfish (including frozen or salted), salted fish, dried fish and shellfish, preserved fish, preserved fish and shellfish, fish products , canned and bottled aquatic products, edible fish meal, processed fish and shellfish products, canned fish, smoked shellfish, processed/frozen/dried/cooked fruits and vegetables, dried fruits [dried fruits], dried Vegetables [dried vegetables], fruit processed food

Fermented red ginseng, processed ginseng food, ginseng jam, ginseng slices, ginseng jelly, processed red ginseng food, red ginseng powder, red ginseng jam, red ginseng slices, red ginseng jelly, edible jelly, jelly, health supplement food with antler as its main ingredient, edible milk Powder, edible colostrum, dried milk for food, milk substitute, milk processed food

The present invention will be described in detail through experimental examples below.

[Experimental Example 1] Production of probiotics

The strains used in this experiment were Lactobacillus plantarum IDCC 3501 isolated from kimchi, Lactobacillus paracasei IDCC 3401 isolated from feces of breastfed infants, and Lactobacillus paracasei IDCC 3401 isolated from homemade cheese. Lactococcus lactis IDCC 2301.

All probiotic strains were cultured in MRS medium, and 10 ⁸ ~ 10 ⁹ CFU/mL bacteria cultured at 37° C. for 24 hours were used in the experiment.

[Experimental Example 2] Confirmation of protease activity of Lactobacillus plantarum IDCC 3501

1) Confirmation of protease activity

Protease activity related to protein degradation was measured by the following method. As the strain for the test, a strain cultured according to the conditions in Experimental Example 1 was used. Experiments were conducted using 10 μl of each sample, and protease activity was distinguished by the formed clear zone. In order to confirm the protease activity, a culture medium using vegetable protein, pea protein, and animal protein, skim milk, was used. Each culture medium was made of protein 28g/L, tryptone 5g/L, yeast extract 2.5g/L, glucose 1g/L, and agar 15g/L, and protein + 50% D.W, the rest of the composition + 50% D.W to prevent protein coagulation. After sterilization, it was mixed and used. After dropping 10 ml of probiotics on the protein culture medium, they were incubated at 37 ° C for 48 hours, and the protease activity was evaluated by measuring the length of the clear zone generated by protein decomposition around the probiotics spot after culturing.

As shown in Figures 1 and 2, Lactobacillus plantarum IDCC 3501 showed protease activity similar to that of other bacteria in pea protein medium, but showed significantly superior protease activity in skim milk medium.

Claims (4)

In the food composition having protein degrading ability,
A food composition comprising Lactobacillus plantarum IDCC 3501 [Accession Number: ATCC BAA-2838] isolated from kimchi and having protease activity. According to claim 1,
A food composition comprising 0.01% to 50% by weight of the Lactobacillus plantarum IDCC 3501. In the health functional food having protein degradation ability,
A health functional food containing Lactobacillus plantarum IDCC 3501 [accession number: ATCC BAA-2838], which was isolated from kimchi and has protease activity, as an active ingredient. According to claim 1,
A health functional food comprising 0.1% by weight to 100% by weight of the Lactobacillus plantarum IDCC 3501.

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