KR102511133B1 - Composition for Inhibiting Biofilm Formation Comprising Extracts of Coptis chinensis - Google Patents

Abstract

The present invention relates to a composition for inhibiting biofilm formation comprising a barberry extract as an active ingredient.
The yellow lotus extract of the present invention has no cytotoxicity, inhibits the activity of the glucosyltransferase enzyme secreted by oral microorganisms such as Streptococcus mutans strains, and effectively inhibits the biofilm formed by oral microorganisms such as Streptococcus mutans strains. Since it can inhibit, it can be usefully used for preventing or treating oral diseases caused by bacterial biofilm formation.

Description

Composition for Inhibiting Biofilm Formation Comprising Extracts of Coptis chinensis}

The present invention relates to a composition for inhibiting biofilm formation containing a Barberry root extract and a composition for preventing, improving or treating oral diseases containing a Barberry root extract.

A biofilm, also called a biofilm, refers to a state in which microorganisms produce polysaccharides around cells and aggregate with adjacent microorganisms to form a film on a solid or biological surface.

Microorganisms that form biofilms are reported to be more than 1,000 times more resistant to antibiotics than suspended microorganisms, and biofilm formation can lead to bacterial endocarditis, dental caries, and chronic sinusitis. , It is well known that it plays an important role in the pathogenesis of various diseases such as food poisoning and pneumonia (Non-Patent Document 1).

On the other hand, dental caries (cavity) is caused by Streptococcus mutans (S.mutans) attached to the tooth surface in the oral cavity, and the S. mutans strain secretes a glucosyltransferase (glucosyltransferase) enzyme. The secreted glucosyltransferase enzyme decomposes sucrose into glucose and fructose, and uses glucose to synthesize polysaccharide glucan. The glucan thus synthesized helps other microorganisms attach, and as a result, many glucans are formed on the tooth surface, increasing the volume of plaque, a type of biofilm. The S. mutans strain attached to teeth by forming a biofilm has resistance against external physical and chemical treatment for hygiene, so it can stay in the oral cavity for a long time. As such, the S. mutans strain, which is not completely removed, continuously acts and causes chronic diseases and complications in the oral cavity.

Therefore, it can be seen that the formation of biofilm is effectively inhibited when the activity of glucosyltransferase is inhibited.

In addition, since biofilms cause diseases such as dental caries or intractable infections in living tissues, it is necessary to effectively inhibit biofilm formation in order to prevent the development of diseases. need something

Dental treatment agents currently used clinically are known to exhibit various side effects. Calcium hydroxide, chlorhexidine, and triple antibiotics, which are used as representative dental treatment agents, exhibit side effects such as tissue necrosis, induction of paresthesia, induction of tooth discoloration, and induction of allergic reactions. In addition, acid/alkali wash, chlorine, and peracid sanitizer, which are chemical methods, have a risk of corrosion when used on metal materials. In particular, it has been reported that chlorine-based sterilization disinfectants, which are frequently used for sterilization treatment, generate carcinogens such as trihalomethane. On the other hand, after sterilization, a large amount of washing water is required in the washing process, and re-contamination of pathogenic microorganisms may occur in the sterilized and disinfected food processing equipment by reusing the washing water.

As such, dental treatment used to remove biofilm has a limitation in that it exhibits side effects on the human body, and accordingly, there is a need to develop a biocompatible new dental treatment that can efficiently control biofilm without side effects.

In addition, as natural products with fewer side effects and improved therapeutic effects have recently received attention, it is necessary to develop natural extracts for the purpose of removing biofilms, but research on natural extracts effective for removing biofilms is still insufficient. .

Under this background, the present inventors studied the therapeutic or preventive effects of oral diseases targeting various plant extracts, and the barberry extract effectively inhibited the formation of biofilms, while blocking the activity of glucosyltransferase to prevent biofilm formation. It was confirmed that it was effective in fundamentally blocking, and the present invention was completed.

Republic of Korea Patent Registration No. 10-1506858 Republic of Korea Patent Registration No. 10-1929565 Korean Patent Publication No. 10-2020-0137316

http://www.intron.co.kr/sub/technologies/biofilm.php

Accordingly, a main object of the present invention is to provide a composition for inhibiting biofilm formation comprising a Coptis chinensis extract as an active ingredient.

Another object of the present invention is to provide a composition for preventing or improving oral diseases comprising an extract of Coptis chinensis as an active ingredient.

Other objects and advantages of the present invention will become more apparent from the following detailed description of the invention, claims and drawings.

According to one aspect of the present invention, the present invention provides a composition for inhibiting biofilm formation comprising a Coptis chinensis extract as an active ingredient.

The present inventors have conducted experiments for many years to search for substances derived from natural materials that are effective in inhibiting the formation of biofilms and preventing or treating oral diseases such as dental caries, and have performed oral cavity extracts of hundreds of various plants. The effect on the disease was verified. As a result, the present invention was completed by confirming that the Barberry extract effectively inhibits the formation of biofilm, while blocking the activity of glucosyltransferase to fundamentally block biofilm formation.

The composition of the present invention is safe without side effects even when applied to the human body because it contains as an active ingredient the extract of barberry root derived from safe natural plant materials without side effects.

" Coptis chinensis " of the present invention refers to an evergreen perennial plant of the dicotyledonous plant Buttercup Orchidaceae. Barberry is known to have effects such as strengthening blood pressure, promoting digestion, preventing food poisoning and diarrhea, and detoxifying. However, the effect of inhibiting the formation of biofilm by microorganisms has not been previously known.

As used in the present invention, the term "extract" refers to an extract obtained by extraction of Barberry, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof, or the like. It includes extracts of all formulations that can be formed using the extract itself and the extract solution. The extract of the present invention may be preferably prepared and used in the form of a dry powder after extraction. In the extraction of the barberry, a method for extracting the extract is not particularly limited, and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method include a hot water extraction method, an ultrasonic extraction method, a filtration method, a reflux extraction method, and the like, which may be performed alone or in combination with two or more extraction methods.

In the present invention, the type of extraction solvent used to extract the barberry is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the extraction solvent include water; C1 to C4 lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; and hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or a mixture thereof may be used, and preferably, water, lower alcohol, 1,3-butylene glycol, and ethyl acetate may be used alone or in combination of two or more.

In the present invention, the extract obtained by hot water extraction or cold brewing is filtered to remove floating solid particles by filtering out the particles using, for example, nylon or by using cryofiltration, and then used as it is or freeze-dried, hot-air-dried, It can be used after being dried using spray drying or the like.

As used herein, the term "fraction" refers to a product obtained by performing fractionation to separate a specific component or a specific component group from a mixture containing various components.

A non-limiting example of the fractionation method may be a method of obtaining fractions from the extract by treating the extract obtained by extracting Barberry lotus with a predetermined solvent. In the present invention, the type of solvent used to obtain the fraction is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of the fractionation solvent include polar solvents such as water and alcohol; and non-polar solvents such as hexane, ethyl acetate, chloroform, and dichloromethane. These may be used alone or in combination of two or more. When alcohol is used among the fractionation solvents, C1 to C4 alcohols may be specifically used.

The extract of the yellow lotus of the present invention can be extracted from any one or more parts of the outpost, leaves, stems, and roots of the yellow lotus, but it is preferable to extract the root. In addition, the barberry extract can be extracted from natural, hybrid or mutant plants, and can also be extracted from plant tissue culture.

In the present invention, the barberry extract is effective in inhibiting biofilm formation by itself.

As used herein, the term "biofilm" refers to a membrane-like structure formed by microorganisms, and has a structural feature consisting of interconnected microbial cell layers and microbial secretions. Microorganisms secrete polysaccharide-generating enzymes to the outside of cells to form water-soluble or insoluble polysaccharides from sugar substances present in the external environment, and when these insoluble polysaccharides are continuously accumulated, a thin film-like biofilm is formed. The formed biofilm helps in aggregation between microorganisms, and as a result may cause diseases such as dental caries.

According to one embodiment of the present invention, the biofilm is Streptococcus sp. , Lactobacillus sp. , Actinobacillus sp. , Aggregatibacter sp. ), Porphyromonas genus ( Porphyromonas sp. ), Tannerella genus ( Tannerella sp. ), Treponema genus ( Treponema sp. ), Prevotella genus ( Prevotella sp. ) and Fusobacterium genus ( Fusobacterium sp. ). ), but may be formed by one or more microorganisms selected from the group consisting of, but is not limited thereto.

In addition, the biofilm is Streptococcus mutans , Streptococcus salivarius, Streptococcus salivarius, Streptococcus gordonii , Streptococcus sobrinus, Streptococcus sanguis ), Streptococcus pyogenes , Lactobacillus acidophilus, Lactobacillus casei , Actinobacillus viscosus , Aggregatibacter actinomycetum comitans ( Aggregatibacter actinomycetemcomitans ), Porphyromonas gingivalis ( Porphyromonas gingivalis ), Tannerella Forsythia ( Tannerella forsythia ), Treponema denticola ), Prevotella intermedia ( Prevotella intermedia ) and Fusobacterium Nucleatum ( Fusobacterium nucleatum ) It may be formed by a microorganism selected from the group consisting of, but is not limited thereto, and preferably may be formed by a Streptococcus mutans strain.

According to another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating oral diseases comprising an extract of Coptis chinensis as an active ingredient. The barberry extract of the present invention is effective in inhibiting biofilm formation by itself, and is effective in treating and preventing oral diseases caused by the formation of bacterial biofilm. Bacteria that form the bacterial biofilm that causes the oral disease are as described above, and are omitted here to avoid overly complicating the specification with excessive description.

In the pharmaceutical composition for preventing or treating oral diseases of the present invention, a representative example of the oral microorganism is Streptococcus mutans ( Streptococcus mutans ), and oral diseases are Streptococcus mutans ( Streptococcus mutans ) Biofilm by the strain It may be an oral disease caused by the formation of a biofilm.

The Streptococcus mutans strain is an important causative agent that attaches to the tooth surface in the oral cavity and causes dental caries (cavity), synthesizes a polysaccharide called glucan using glucose, and forms a biofilm with the generated glucan to treat teeth By attaching to the surface, it has resistance against external physical and chemical treatment.

Since the yellow lotus extract of the present invention can inhibit the activity of glucosyltransferase secreted by oral microorganisms such as Streptococcus mutans strains and effectively inhibit biofilms formed by oral microorganisms such as Streptococcus mutans strains, It can be usefully used to effectively suppress dental caries, which can be caused by biofilms and plaques, and thus stomatitis and bad breath.

Therefore, in the pharmaceutical composition for preventing or treating oral diseases of the present invention, the oral diseases may be dental caries or stomatitis.

The pharmaceutical composition for preventing or treating oral diseases of the present invention may be in the form of a pharmaceutical composition containing a pharmaceutically acceptable carrier. The pharmaceutical composition may be formulated in the form of oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc. according to conventional methods, and powders, tablets, capsules, and liquids are more preferred. desirable. Such formulation may be performed by a method commonly performed in the field of pharmaceuticals, and preferably formulated according to each disease or added component using a method disclosed in Remington's Pharmaceutical Science (Mack Publishing Company, Easton PA) can do.

The pharmaceutically acceptable carrier is lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil; and the like.

Solid preparations for oral use include tablets, pills, powders, granules, capsules, etc., and these solid preparations contain at least one or more excipients, for example, starch, calcium carbonate, sucrose or lactose. ), gelatin, etc., magnesium stearate, lubricants such as talc, and the like. Oral liquid preparations include suspensions, internal solutions, emulsions, syrups, and the like, and may include diluents such as water and liquid paraffin, wetting agents, sweeteners, aromatics, and preservatives.

The oral composition of the present invention may be formulated into, for example, a film, toothpaste, mouthwash solution, ointment, spray, dressing solution, coating agent, dental floss, or periodontal bag, in addition to the powder, tablet, or capsule formulation.

In the pharmaceutical composition of the present invention, the barberry extract may be included in an amount of 0.1 to 50% by weight, preferably 1 to 20% by weight, based on the total weight of the composition, and method of using the pharmaceutical composition for preventing or treating oral diseases and Depending on the purpose of use, the content of the active ingredient can be appropriately adjusted.

The pharmaceutical composition of the present invention can be administered orally according to the desired method, and the dosage ranges depending on the patient's weight, age, sex, health condition, diet, administration time, administration method, excretion rate, and severity of the disease. is diverse.

The daily dose of the Barberry extract of the present invention is about 0.1 to 500 mg/kg, preferably 50 to 200 mg/kg.

The pharmaceutical composition of the present invention can be used alone or in combination with methods using surgery and drug treatment for the prevention and treatment of oral diseases, for example, preventing tooth decay, preventing periodontal disease, preventing gingivitis, and removing bad breath. It is for the effect of fluoride, zinc chloride, chlorhexidine, aminocaproic acid, tranexamic acid, cetylpyridium chloride, pyridoxine chloride, triclosan, tocopherol acetate, sodium monofluorophosphate, etc., but is not limited thereto, and these alone It can be used as or in combination of two or more.

According to another aspect of the present invention, the present invention provides a food composition for preventing or improving oral diseases comprising an extract of Coptis chinensis as an active ingredient.

The yellow raspberry extract of the present invention has no cytotoxicity, is effective in inhibiting biofilm formation by itself, and is effective in preventing and improving oral diseases caused by the formation of bacterial biofilms, so it is used for preventing or improving oral diseases. It can be usefully used as a food composition. The effects of the extract of the present invention on the prevention and improvement of oral diseases are as described above. The food composition may be used in the form of health functional food, but is not limited thereto.

The food composition of the present invention may be included in the form of a fraction of Barberry extract or a processed product thereof. In addition, the composition may include a food additive that is acceptable in food science in addition to the active ingredient.

In the present invention, "food supplement additive" refers to a component that can be added to food supplementally, and can be appropriately selected and used by those skilled in the art as being added to prepare a health functional food of each formulation. Examples of food additives include various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, colorants and fillers, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners , pH regulators, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, etc. are included, but the types of food additives of the present invention are not limited by the above examples.

The food composition of the present invention may include health functional food. In the present invention, "health functional food" refers to food manufactured and processed in the form of tablets, capsules, powders, granules, liquids and pills using raw materials or ingredients having useful functionality for the human body. Here, "functionality" means obtaining useful effects for health purposes such as adjusting nutrients for the structure and function of the human body or physiological actions, and specifically means the effect of preventing or improving oral diseases. The health functional food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and components commonly added in the art during the preparation.

In addition, the formulation of the health functional food may also be manufactured without limitation as long as the formulation is recognized as a health functional food. The food composition of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that may occur when taking drugs for a long time by using edible Barberry extract as a raw material, and is effective in the action of oral microorganisms. Since it suppresses the formation of biofilm in the oral cavity by and suppresses the activity of glucosyltransferase, and thus has excellent effect on improving oral diseases, it can be taken as an adjuvant to enhance the therapeutic effect of oral diseases.

There is no limit to the form that the health functional food of the present invention can take, and it may include all foods in a conventional sense, and may be used interchangeably with terms known in the art, such as functional foods. In addition, the health functional food of the present invention may be prepared by mixing suitable other auxiliary ingredients and known additives that may be included in food according to the selection of those skilled in the art. Examples of foods that can be added include beverages, tea, drinks, alcoholic beverages, meat, sausages, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, dairy products including ice cream, various soups, And vitamin complexes, etc., which can be prepared by adding to juices, teas, jellies, juices, etc. prepared using the barberry extract according to the present invention as a main component. Also included are foods used as feed for animals.

Since the composition of the present invention is effective in preventing and improving oral diseases by itself, it can be directly used as a composition for preventing and improving oral diseases, and is used in combination with other ingredients containing natural extracts effective for oral diseases. It is expected that it can be used in the form of

The features and advantages of the present invention are summarized as follows:

(1) The present invention provides a composition for inhibiting biofilm formation comprising a barberry extract as an active ingredient.

(2) Barberry extract of the present invention is not cytotoxic, inhibits the activity of the glucosyltransferase enzyme secreted by oral microorganisms such as Streptococcus mutans strains, and is formed by oral microorganisms such as Streptococcus mutans strains. Since it can effectively inhibit the film, it can be usefully used for preventing or treating oral diseases caused by the formation of bacterial biofilms.

1 is a graph showing the measurement of changes in biofilm production according to the treatment of Barberry extract.
Figure 2 is a graph showing the measurement of changes in glucosyltransferase enzyme activity according to the treatment of barberry extract.

Hereinafter, the configuration of the present invention will be described in more detail with examples, but the scope of the present invention is not limited to the embodiments disclosed below, but will be implemented in various forms different from each other, only the present embodiments It is provided to make the disclosure of the invention complete, and to fully inform those skilled in the art of the scope of the invention to which the present invention belongs, and the present invention is only defined by the scope of the claims. In addition, the significance between the control group, comparison group, and experimental group according to the experimental results described below was verified by One-Way ANOVA.

Example 1. Preparation of Barberry Extract

200 ml of 70% ethanol was added to 20 g of Barberry root, respectively, and reflux extraction was performed in a constant temperature water bath at 100 ° C. for 3 hours. Thereafter, the extract was filtered using filter paper, and concentrated at 40° C. or lower and used in the experiment.

Example 2. Biofilm formation inhibitory activity of barberry extract

Streptococcus mutans ( Streptococcus mutans ; hereinafter referred to as "S. mutans") strain was distributed from the National Institute of Agricultural Sciences Microorganism Bank (KACC) and cultured in BHI (Brain Heart Infusion medium, Bacto ™, BD Biosciences) medium.

The S. mutans strain was streaked on a BHI agar plate and then cultured at 37° C. for 2 days. A single colony was taken from the plate and inoculated into 5 ml of BHI broth, followed by stationary culture at 37°C for 1 day.

100 μl of BHI medium containing 1% sugar was dispensed into a 96-well plate (SPL), and 1 μl of Barberry extract prepared in Example 1 was additionally dispensed. Next, each well was inoculated with the statically cultured S. mutans strain to an OD600 of 0.05, and incubated at 37° C. for 24 hours. After 24 hours, the optical density (OD; absorbance) was measured at OD595 to confirm the cell density of the S. mutans strain. After removing the culture medium from the plate, the plate was gently washed three times with distilled water.

100 μl of 1% crystal violet solution was dispensed into each well, and the biofilm was stained for 15 minutes at room temperature. After 15 minutes, it was washed three times with distilled water, and 100 μl of 95% ethanol was added to elute the crystal violet, and left for 15 minutes. Then, the amount of the biofilm formed was quantified by measuring the absorbance at OD595 with a microplate reader, and summarized in FIG. 1 .

Referring to FIG. 1, it was confirmed that the amount of biofilm was significantly reduced in the experimental group to which the Barberry extract was added, and in the test group treated with the Barberry extract of the present invention, 96.71% ( 31.3 μg/ml), 73.3% (62.5 μg/ml), 29% (125 μg/ml), 25% (250 μg/ml), and 11% (500 μg/ml) of biofilm production were measured. .

Through the above experimental results, it was confirmed that the extract of the present invention has an excellent effect in inhibiting the biofilm produced by Streptococcus mutans.

Example 3. Glucosyltransferase inhibitory activity of barberry extract

3-1. Production and isolation of glucosyltransferase enzymes from S. mutans strains

Since the S. mutans strain secretes glucosyltansferase (hereinafter referred to as “GTase”) enzyme to the outside of the cell, the GTase enzyme produced by the S. mutans strain was isolated by the following method.

First, a single colony was taken from the plate and the S. mutans strain was inoculated into 40 ml of BHI broth, followed by culturing at 37° C. for 24 hours. After 24 hours, absorbance was measured at 600 nm with a spectrophotometer, and S. mutans strain was inoculated to OD600 of 0.05 in 1 L of fresh BHI liquid medium. The S. mutans strain was cultured in a fresh BHI liquid medium for 24 hours to produce the GTase enzyme.

After 24 hours, the culture solution was centrifuged at 4° C. for 10 minutes at 1,500 x 10 g to collect the supernatant containing the GTase enzyme. The supernatant collected to precipitate the protein was mixed with 95% ethanol stored in a refrigerator (4°C) at a ratio of 1:1, and reacted in a refrigerator at 4°C for 24 hours. Next, the supernatant was removed by centrifugation at 4° C. for 10 minutes at 1,500 x 10 g, and only the precipitated proteins were collected. The collected proteins were dissolved again using 10 ml of 60 mM potassium phosphate buffer (pH 6.8), washed, and centrifuged under the same conditions as above. Thereafter, the supernatant was removed, and the precipitate was again dissolved in 10 ml of 60 mM potassium phosphate buffer, and then dispensed by 1 ml and stored at -20 ° C.

3-2. Measurement of GTase enzyme activity inhibition ability of Barberry root extract

Glucosyltransferase (GTase) decomposes sucrose into glucose and fructose in the oral cavity, and synthesizes a polysaccharide called glucan using glucose. The generated glucan helps attach microorganisms to the tooth surface, and as a result, many glucans are formed on the tooth surface, increasing the volume of plaque, a type of biofilm. Therefore, it can be seen that the formation of biofilm is inhibited when the activity of glucosyltransferase is inhibited.

The enzymatic activity inhibitory ability of the Barberry root extract was measured using the GTase enzyme prepared in Example 3-1.

Since the GTase enzyme breaks down sugar into glucose and converts glucose into glucan, 60 mM potassium phosphate buffer (containing 1.25% sugar and 0.025% NaN ₃ , pH 6.8) containing sugar was used as a substrate to measure the enzyme reaction. did Specific enzyme reaction conditions are described in [Table 1], and reacted at 37 ° C. for 24 hours.

(Unit: μl) temperament GTase extract Distilled water menstruum Sum blank test group 800 0 50 100 0 1000 negative control group 800 50 0 150 50 experimental group 800 50 50 100 0

After the reaction was completed, the glucan was released using a sonicator for 5 seconds. The supernatant was removed by centrifugation at 4° C. for 10 minutes at 1,500 x 10 g, and 200 μl of distilled water was added to the precipitate, and then sonication was performed for 3 seconds using a sonicator to release the precipitate again. .

200 μl was dispensed into a 96-well plate (SPL), and the amount of glucan produced was measured by measuring the turbidity at an absorbance of 540 nm using a microplate reader. Organized.

Referring to Figure 2, it was confirmed that the amount of glucan production was significantly reduced in the experimental group to which the Barberry extract of the present invention was added, and in the test group treated with the Barberry extract of the present invention, compared to the negative control group not treated with the Barberry lotus extract, respectively, 94.47 % (31.3 μg/ml), 83.3% (62.5 μg/ml), 50% (125 μg/ml), 48% (250 μg/ml), and 46% (500 μg/ml) of glucan production has been measured

Through the above experimental results, it was confirmed that the extract of the present invention inhibits the activity of the glucosyltransferase (GTase) enzyme to reduce the production of glucan, thereby having an excellent effect on inhibiting biofilm.

Claims (6)

Barberry ( Coptis chinensis ) Containing the extract as an active ingredient,
The extract of Barberry is characterized in that it is an extract obtained by refluxing extract of Barberry with 10 times (w / v) of 70% ethanol at 100 ° C. for 3 hours,
Characterized in that it inhibits the formation of a biofilm formed by Streptococcus mutans ,
A composition for inhibiting biofilm formation. delete Barberry ( Coptis chinensis ) Containing the extract as an active ingredient,
The extract of Barberry is characterized in that it is an extract obtained by refluxing extract of Barberry with 10 times (w / v) of 70% ethanol at 100 ° C. for 3 hours,
It is characterized by inhibiting the formation of a biofilm formed by Streptococcus mutans ,
Streptococcus mutans ( Streptococcus mutans ) A pharmaceutical composition for preventing or treating oral diseases caused by biofilm formation. delete delete Barberry ( Coptis chinensis ) Containing the extract as an active ingredient,
The extract of Barberry is characterized in that it is an extract obtained by refluxing extract of Barberry with 10 times (w / v) of 70% ethanol at 100 ° C. for 3 hours,
It is characterized by inhibiting the formation of a biofilm formed by Streptococcus mutans ,
Streptococcus mutans ( Streptococcus mutans ) A food composition for preventing or improving oral diseases caused by biofilm formation.

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