KR102506883B1 - Method for evaluating sun protection degree - Google Patents

Abstract

The present invention is a sun protection factor (SPF) evaluation method, comprising the steps of: (1) irradiating natural pigments with at least one artificial ultraviolet light having a different light intensity value so as to transmit each test product; (2) measuring the absorbance of natural pigment irradiated with artificial ultraviolet rays through the test product; and (3) deriving the UV blocking power of the test product from the measured absorbance data. Specifically, according to the present invention, a standardized method for evaluating UV blocking power is provided. In addition, the present invention has the effect of objectively and accurately evaluating the UV blocking power.

Description

UV protection evaluation method {METHOD FOR EVALUATING SUN PROTECTION DEGREE}

The present invention relates to a method for evaluating UV blocking power, and more particularly, to a method for evaluating the UV blocking power of a product to be tested, which can objectively and accurately evaluate the UV blocking power of a product to be tested.

Ultraviolet light refers to a part corresponding to a wavelength range of approximately 280 nm to 400 nm in the spectrum of sunlight. Ultraviolet rays are classified into UVA, UVB and UVC depending on the wavelength. Excessive exposure to ultraviolet rays is known to accelerate skin aging and, in severe cases, to cause DNA damage and even skin cancer. In order to reduce skin damage caused by such ultraviolet rays, a product having a sunscreen effect, that is, a sunscreen product, has been steadily developed.

In general, the efficacy of sunscreens is evaluated through a sun protection factor (SPF) index and a protection factor of UVA (PA) index. SPF and PA are used as evaluation indices for UV blocking power. SPF is used to indicate UVB blocking power, and PA is used to indicate UVA blocking power. There are currently various methods for determining the UV blocking power, but the representative test method that is widely used and recognized is the most common in vivo method for humans.

However, the in vivo method for determining the UV blocking power has various problems such as ethical problems such as protection of subjects, high cost and time-consuming economic problems, and deviation of values within and between experiments, and thus substitutes for these Various attempts have been made to do this, but there is currently no representative in vitro test method that can replace it.

Republic of Korea Patent Publication No. 10-2012-0049634

Accordingly, an object of the present invention is to provide a method for evaluating the UV blocking power of a test product that can objectively and accurately evaluate the UV blocking power of a product to be tested.

In order to achieve the above object, one embodiment of the present invention is a method for evaluating the sun protection factor (SPF) of a test product,

(1) irradiating natural pigments with artificial ultraviolet rays such that at least one artificial ultraviolet light having different light intensity values first penetrates the test product and then reaches the natural pigment;

(2) measuring the absorbance of natural pigment irradiated with artificial ultraviolet rays through the test product; and

(3) It provides a method for evaluating the UV blocking power of the test product, including the step of deriving the UV blocking power of the test product from the measured absorbance data.

According to the present invention, a standardized method for evaluating ultraviolet ray blocking power is provided. In addition, the present invention has the effect of objectively and accurately evaluating the UV blocking power of the test product.

1A to 1D are photographs showing color changes of pigments according to the amount of UV irradiation and the UV protection index evaluated according to an embodiment of the present invention (FIG. 1A sample uncoated, FIG. 1B SPF15 applied, FIG. 1C SPF30 applied, FIG. 1d SPF50 applied).
2a to 2d are graphs showing the results of measuring the absorbance of pigments according to the amount of UV irradiation and the UV protection index evaluated according to an embodiment of the present invention (Fig. 2a sample uncoated, Fig. 2b SPF15 coated, Fig. 2c SPF30 coated, Fig. 2d SPF50 application).

As described above, a method for evaluating the UV blocking power has not yet been standardized. In addition, it is not objective or accurate to evaluate the UV blocking power by conventional in vivo test methods for humans.

Therefore, as a result of repeated studies on the determination of objective UV blocking power, it was found that UV blocking power can be accurately and objectively evaluated by using natural pigments that react more sensitively to UV rays and quantifying the color change of the pigment with absorbance measurement values. Could know.

Hereinafter, the present invention will be described in detail.

In the method for evaluating UV blocking power of a test product according to the present invention, after artificial UV rays are transmitted through a natural dye to pass through the test product, the UV blocking power of the test product is derived from the absorbance measurement result of the natural dye.

In this case, the sun protection factor may be derived by determining a sun protection factor such as SPF (sun protection factor) or PA (protection factor of UVA).

According to a specific embodiment, the method for evaluating the UV blocking power of the test product according to the present invention may include the following steps (1) to (3).

(1) irradiating natural pigments with at least one artificial ultraviolet light having different light intensity values so that each of the artificial ultraviolet rays first penetrates the test product and then reaches the natural pigment

(2) measuring the absorbance of natural pigment irradiated with artificial ultraviolet rays through the test product

(3) deriving the UV blocking power of the test product from the measured absorbance data

In the step (1), the at least one artificial ultraviolet light irradiation may be performed simultaneously or sequentially for at least one natural pigment dispensed into each container. That is, at least one artificial ultraviolet light having a different light intensity value may be simultaneously irradiated to at least one natural pigment distributed in each container or sequentially irradiated to each natural pigment according to the light intensity value.

On the other hand, according to one embodiment, the irradiation of the artificial ultraviolet ray applies the test product to a membrane or plate through which a sunscreen product can be applied and the artificial ultraviolet ray passes, and it is applied to the artificial ultraviolet ray and By placing it between natural pigments and then irradiating artificial ultraviolet rays, the natural pigments can be irradiated so as to transmit artificial ultraviolet rays through the test product.

For example, a poly methylmethacrylate (PMMA) plate may be used, but is not necessarily limited thereto, and various membranes and plates may be used if a UV blocking product can be applied without affecting the wavelength and intensity of the UV band.

According to another embodiment, the irradiation of the artificial ultraviolet light may be applied to the natural pigment on which the test product is applied on the surface, or the natural pigment is printed with an inkjet printer, the test product is applied thereon, and then the artificial ultraviolet light is irradiated. can do.

More specifically, for example, when printing natural pigments with the inkjet printer, titanium dioxide (TiO ₂ ), which serves as a catalyst, and polyvinylpyrrolidone (PVP), which serves as a binder for forming a film, are mixed with natural pigments. It is preferable to print together.

The irradiation of the at least one artificial ultraviolet light may be irradiated with ultraviolet light corresponding to a specific light intensity through an artificial light source. The artificial light source may be, for example, a general lamp or an ultraviolet emitter.

At this time, the irradiation intensity of each of the at least one or more artificial ultraviolet rays, that is, the light intensity value and the irradiation time are not particularly limited, but are 0.1 to 100 mJ/cm ² ·min, preferably 0.5 to 50 mJ/cm ² ·min. can be investigated by the intensity of For example, the artificial ultraviolet ray is 0.1 mJ/cm ² min or more, 0.5 mJ/cm ² min or more, 1.0 mJ/cm 2 min or more, 5.0 mJ/cm ^{2 min} or more, 10 mJ/cm ^{2 min} or more. min or more, 15 mJ/cm ² min or more, 20 mJ/cm ² min or more, 25 mJ/cm ² min or more, 30 mJ/cm ^{2 min} or more, 35 mJ/cm ^{2 min} or more, 40 mJ /cm ² min or more, 45 mJ/cm ² min or more, 50 mJ/cm ² min or more, 60 mJ/cm ² min or more, 70 mJ/cm ² min or more, 80 mJ/cm ^{2 min} or more or 90 mJ/cm ² min or more, 100 mJ/cm ² min or less, 90 mJ/cm ² min or less, 80 mJ/cm ² min or less, 70 mJ/cm ² min or less, 60 mJ/cm ² min or less, 50 mJ/cm ² min or less, 45 mJ/cm ² min or less, 40 mJ/cm ² min or less, 35 mJ/cm ² min or less, 30 mJ /cm ² min or less, 25 mJ/cm ² min or less, 20 mJ/cm ² min or less, 15 mJ/cm ² min or less, 10 mJ/cm ² min or less, 5.0 mJ/cm ² min or less Or less, 1.0 mJ/cm ² ·min or less or 0.5 mJ/cm ² ·min or less may be irradiated with an intensity.

In addition, the irradiation time may be 30 to 90 minutes, preferably 50 to 70 minutes. For example, the artificial ultraviolet rays are irradiated for 30 minutes or more, 35 minutes or more, 40 minutes or more, 45 minutes or more, 50 minutes or more, 55 minutes or more, 60 minutes or more, 65 minutes or more, 70 minutes or more, or 80 minutes or more. 90 minutes or less, 80 minutes or less, 70 minutes or less, 65 minutes or less, 60 minutes or less, 55 minutes or less, 50 minutes or less, 45 minutes or less, 40 minutes or less, or 35 minutes or less. .

In addition, the total amount of light (light intensity x irradiation time) is not particularly limited, but may be 1.0 to 5000 mJ/cm ² , preferably 30 to 3000 mJ/cm ² . For example, the artificial ultraviolet light has a total light intensity of 1.0 mJ/cm ² or more, 10 mJ/cm ² or more, 30 mJ/cm ^{2 or} more, 50 mJ/cm ^{2 or} more, 100 mJ/cm ^{2 or} more, or 300 mJ/cm ² or more, 500 mJ/cm ² or more, 1000 mJ/cm ^{2 or} more, 1500 mJ/cm ² or more, 2000 mJ/cm ^{2 or} more, 2500 mJ/cm ² , 300 mJ/cm ² or more, 3500 mJ/cm ² or more , 4000 mJ/cm ² or more, or 4500 mJ/cm ² or more, 5000 mJ/cm ² or less, 4500 mJ/cm ^{2 or} less, 4000 mJ/cm ² or less, 3500 mJ/cm ² or less, 3000 mJ/cm ² or less, 2500 mJ/cm ² or less, 2000 mJ/cm ^{2 or less, 1500 mJ/cm 2 or} less, 1000 mJ/cm ^{2 or} less, 500 mJ/cm ² or less, 300 mJ/cm ² or less, 100 mJ/cm ² or less, 50 mJ/cm ² or less, 30 mJ/cm ² or less, or 10 mJ/cm ^{2 or} less may be irradiated.

In one embodiment, the natural pigment is a pigment that reacts sensitively to ultraviolet rays and exhibits color change and absorbance change, and is a carotenoid, porphyrin, diketone, beta-cyanine (β) -cyanin) and asapilone (ASA pyrone).

Preferably, the natural pigment may be a porphyrin-based pigment, in particular, spirulina blue color.

Examples of the carotenoid-based pigment include, but are not limited to, annatto pigments containing bixin, norbixin, etc. as main components; Beta-carotene (β-carotene), beta-apo-8'-carotene (β-Apo-8'-carotenal), lycopene (lycopene), carotene (carotene) pigment containing phytoene (phytoene) as a main component; Paprika pigment containing capsanthin and the like as a main component; and crocin pigments containing crocin, crocetin, and the like as main components.

Examples of the porphyrin-based pigment include, but are not limited to, chlorophyll pigment; and spirulina pigments containing phycocyanin as a main component.

Examples of the diketone-based pigment include, but are not limited to, turmeric pigments containing curcumin or the like as a main component.

Examples of the beta-cyanine pigment include, but are not limited to, beet reed containing isobetanine and betanine as main components.

Examples of the asaphyllone-based pigment include, but are not limited to, Monascus (monascurbin, monasflavin, monasin, ankaflavin, etc. as main components) monascus) pigment.

In one embodiment, in the step (3), the UV blocking power of the test product is derived by comparing the absorbance graph of the natural pigment according to the light amount of the artificial UV light with a standard curve designated in advance for each UV blocking power. can do.

That is, when the shape of the graph of absorbance of natural pigments according to the amount of artificial ultraviolet light measured for the test product matches the standard curve specified in advance for the sunscreen having a specific ultraviolet blocking power, the test product has the specific ultraviolet blocking power. can be determined to have.

In another embodiment, in the step (3), the UV blocking power of the test product may be derived by comparing the slope of the absorbance graph of the natural pigment according to the light amount of the artificial UV light with a pre-specified slope value for each UV blocking power. there is.

That is, when the slope of the absorbance graph of the natural pigment according to the amount of artificial ultraviolet light measured for the test product falls within a predetermined slope range for a sunscreen having a specific UV blocking power, the test product has the specific UV blocking power. can be determined as

In another embodiment, the step (3) is based on the optical density (OD) of the natural pigment irradiated with artificial ultraviolet light passing through the test product and the natural pigment irradiated with artificial ultraviolet light without passing through the test product. The UV blocking power of the test product can be derived from the difference value (OD-blank OD) of the absorbance value (blank OD) of the dye.

For example, when the difference value (OD-blank OD) x 10 is 0.7 to 1.2, the UV blocking power can be determined to be 15, and when the difference value is 1.3 to 1.7, the UV blocking power can be determined to be 30, When the difference value is 1.8 to 2.7, the UV blocking power may be determined to be 50.

As described above, according to the method for evaluating the UV blocking power of the test product of the present invention, the absorbance change of the natural pigment after irradiation of artificial ultraviolet rays through the test product is used as a measure to objectively and accurately measure the UV blocking power of the test product in a short time. can be evaluated Therefore, based on the evaluation results, it may be possible to predict the UV blocking power of a specific product.

Hereinafter, the content of the present invention will be described in more detail through examples. However, these embodiments are only presented to understand the contents of the present invention, the scope of the present invention is not limited to these embodiments, and modifications, substitutions, and insertions commonly known in the art can be performed. It may be, and it is also included in the scope of the present invention.

[Example]

Spirulina blue dye (Spirulina Blue No. 15L, Samyoung Food Co.) was used as a natural pigment, which was diluted in water at a ratio of 1:74 and used after filtering with a 0.2 μm syringe. 500 μL each of the filtered natural pigment diluted in the water was dispensed into 6 containers, and artificial ultraviolet light was irradiated for 60 minutes with the light quantity and light intensity shown in Table 1 below.

Light intensity (MED; MED=21mJ/cm ² min) Light amount (mJ/cm ² ) 0.033 42 0.667 840 1.000 1260 1.333 1680 1.667 2100 2.000 2520

At this time, artificial ultraviolet irradiation was irradiated through a total of six irradiation sites according to each light amount using a multiport simulator (Solar Light Company). In addition, after applying samples having UV blocking powers of SPF15, SPF30, and SPF50 to a PMMA (poly methylmethacrylate) plate, respectively, they are placed in the ultraviolet irradiation unit of the artificial ultraviolet irradiator, so that artificial ultraviolet rays according to each amount of light penetrate the sample, respectively, It was irradiated with the dog's pigment. After that, the color change of each dye was observed and the absorbance was measured three times in total. Visual observation results of color change are shown in FIGS. 1A to 1D , and absorbance measurement results are shown in Table 2 (average value of a total of three absorbance measurements) and FIGS. 2A to 2D .

Light amount (mJ/cm ² ) no sample applied SPF15 SPF30 SPF50 0 0.325 0.327 0.330 0.338 42 0.307 0.323 0.321 0.337 840 0.131 0.250 0.275 0.332 1260 0.111 0.206 0.242 0.330 1680 0.083 0.175 0.176 0.309 2100 0.080 0.171 0.224 0.306 2520 0.066 0.139 0.200 0.319

From the results of FIGS. 1A to 1D, when the sample was not applied (FIG. 1A), the color of cyanide changed to transparent as the amount of light increased (from left to right), and samples of SPF15, SPF30, and SPF50 were applied, respectively. In this case (FIG. 1b, FIG. 1c, and FIG. 1d, respectively), it was confirmed that the degree of color change greatly decreased as the amount of light increased. That is, it was confirmed that the UV blocking power can be objectively and accurately evaluated in the case of using natural pigments that are sensitive to UV rays, that is, change color by UV rays, according to the present invention.

In addition, from the results of Table 2 and FIGS. 2A to 2D, it was confirmed that the decrease in absorbance due to the increase in the amount of light gradually decreased as the UV blocking power increased. That is, it was confirmed that as the UV blocking power increased, the absorbance graph appeared in parallel to the X-axis.

On the other hand, looking at the difference between the absorbance when the sample was applied and the absorbance when the sample was not applied (OD-blank OD) according to each UV blocking power, in the case of SPF15, it is 0.002 to 0.119, and x10 is 0.02 to 1.19 , SPF30 is 0.005 to 0.144, x10 is 0.05 to 1.44, and SPF50 is 0.014 to 0.253, x10 is 0.14 to 2.53.

That is, from the above results, according to the present invention, the absorbance measurement result of the dye after applying artificial ultraviolet rays to transmit the test product, that is, the absorbance graph, the slope of the graph, and the absorbance difference value with the blank control group for each specific ultraviolet blocking power It can be seen that the UV blocking ability of the test product can be objectively and accurately evaluated by comparing the shape of the standard curve specified in advance, the descriptor range, and the absorbance difference value range with the blank control group.

Claims (10)

As a method for evaluating the UV blocking power of the test product,
(1) irradiating natural pigments with artificial ultraviolet rays such that at least one artificial ultraviolet light having different light intensity values first penetrates the test product and then reaches the natural pigment;
(2) measuring the absorbance of natural pigment irradiated with artificial ultraviolet rays through the test product; and
(3) deriving the UV blocking power of the test product from the measured absorbance data;
In the step (3),
comparing a graph of absorbance of the natural pigment according to the amount of artificial ultraviolet light with a standard curve designated in advance for each ultraviolet blocking ability to derive the ultraviolet blocking power of the test product;
Deriving the UV blocking power of the test product by comparing the slope of the absorbance graph of the natural pigment according to the light amount of the artificial UV light with a pre-specified slope value for each UV blocking power; or
The difference between the absorbance value (OD) of the natural pigment irradiated with artificial ultraviolet light passing through the test product and the absorbance value (blank OD) of the natural pigment irradiated with artificial ultraviolet light without penetrating the test product (OD-blank OD) A method for evaluating the UV blocking power of the test product, which derives the UV blocking power of the test product from According to claim 1,
Characterized in that the irradiation of the at least one artificial ultraviolet ray is performed simultaneously or sequentially with respect to at least one or more natural pigments dispensed in each container, the method for evaluating the ultraviolet ray blocking power of the test product. According to claim 1,
Each of the at least one artificial ultraviolet ray is irradiated with an intensity of 0.1 to 100 mJ / cm ² · min, characterized in that, the method of evaluating the ultraviolet ray blocking power of the test product. According to claim 3,
The artificial ultraviolet rays are irradiated for 30 to 90 minutes, characterized in that, a method for evaluating the ultraviolet ray blocking power of the test product. According to claim 1,
Characterized in that the natural pigment is a pigment selected from carotenoids, porphyrins, diketones, β-cyanin and ASA pyrones, the test A method for evaluating the UV blocking power of products. According to claim 5,
Characterized in that the natural pigment is a porphyrin-based pigment, a method for evaluating the UV blocking power of the test product. According to claim 6,
Characterized in that the natural pigment is spirulina blue color, a method for evaluating UV protection of the test product. delete delete delete

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