KR102500295B1 - Method for preparing antioxidant functional composition containing sturgeon water-soluble oil - Google Patents

Abstract

The present invention relates to an antioxidant functional composition and, more particularly, to a method for manufacturing an antioxidant functional composition that has improved flavor and functionality by mixing sturgeon water-soluble oil rich in nutrients such as essential amino acids and excellent in antioxidant and blood circulation improvement effects, and nut paste rich in nutrients such as unsaturated fatty acids and excellent in antioxidant and cholesterol lowering effects.

Description

Method for preparing antioxidant functional composition containing sturgeon water-soluble oil {Method for preparing antioxidant functional composition containing sturgeon water-soluble oil}

The present invention relates to an antioxidant functional composition, and more particularly, to a composition rich in nutrients such as essential amino acids, rich in nutrients such as sturgeon water-soluble oil and unsaturated fatty acids, rich in nutrients such as antioxidant and excellent in antioxidant and blood circulation improvement, antioxidant and cholesterol level It relates to a method for producing an antioxidant functional composition with improved flavor and functionality by mixing nut paste with excellent lowering efficacy.

Sturgeon ( Acipenser sinensis ) is a fish species originating from 450 to 350 million years ago (Devonian Period), belonging to the Acipenseridae family, and is a fish species that is widely distributed in the northern mid-latitude region of the Northern Hemisphere and grows naturally. Currently, 26 species of sturgeon are known, and interest in many countries is increasing due to their economic and ecological value.

In March 1997, Siberian sturgeon, which was first transplanted and bred in Korea at Canakov Farm in Russia, and then produced in 2003 by its own seedlings, has excellent environmental adaptability, grows faster than other breeds farmed in Korea, and has good meat quality. It has become a popular fish for aquaculture. The maximum body length is 3 m, the weight can grow up to 200-300 kg, there are species that grow to a maximum length of more than 8 m, and the maximum life span exceeds 100 years. At about 6 to 7 years old, they grow to more than 1 m and produce caviar.

Sturgeon has a chewy taste of flesh, so it is used in raw, smoked, barbecued, steak and skewered dishes, its head is steamed and stewed, its fin is used in shark fin dish, which is one of the three major delicacies in China, and its cartilage is used for special dishes, tonics and industrial ingredients. there is. In addition, sturgeon contains a large amount of active ingredients such as protein, essential amino acids, unsaturated fatty acids, vitamins, EPA, DHA, etc. As the excellent efficacy of is revealed, it is getting more and more attention as a highly nutritious and highly functional material.

In recent years, as sturgeon farming technology has begun to develop, there are more opportunities for common people to come into contact with sturgeon. It became.

However, in the raw material of sturgeon marketed to date, sufficient research has not been conducted on the actual content of sturgeon, the content of active ingredients, processing method, extraction efficiency, etc. In particular, since there is no processing technology for sturgeon and products using it in Korea, sturgeon has not yet been objectively and publicly recognized by consumers as a functional material and product.

On the other hand, oxidative stress caused by active oxygen and free radicals causes denaturation and destruction of lipids, proteins, DNA, enzymes, etc., which causes aging and various diseases. When the biological defense ability of antioxidants that can withstand such oxidative stress is abnormal or exposed to excessive active oxygen and free radicals, the balance is broken and substrates, proteins, and DNA are irreversibly destroyed, resulting in aging and Various diseases such as cancer, complex arteriosclerosis, arthritis and Parkinson's disease are induced.

Here, what acts as an antioxidant can be divided into enzymatic and non-enzymatic types. Antioxidant enzymes such as superoxide disputase (SOD), catalase, and peroxidase, and vitamins C, E, and glutathione ) and other non-enzymatic substances.

On the other hand, in order to prevent and improve diseases caused by oxidative stress, research on developing antioxidants, inducing the expression of antioxidant enzymes, or developing pharmaceutical preparations and functional foods containing non-enzymatic antioxidants is underway. has been progressing Synthetic antioxidants developed so far include butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT) and nordihydro-guaiaretic acid (NDGA).

However, synthetic antioxidants have disadvantages such as being toxic in vivo, possibly causing allergies and tumors, and being vulnerable to temperature and being easily destroyed once heat is applied. In addition, natural antioxidants such as tocopherol are also being developed, but are known to have the advantage of being safe for the living body compared to synthetic antioxidants, but have a weak effect.

Therefore, it is necessary to develop an anti-oxidant functional composition using natural substances that are not toxic to the living body and have superior antioxidant effects than existing natural antioxidants.

Republic of Korea Patent Publication No. 10-2009-0111066

The present invention is intended to solve the above problems, and is rich in nutrients such as essential amino acids, and is rich in nutrients such as sturgeon water-soluble oil and unsaturated fatty acids, which are rich in antioxidants and have excellent effects in improving blood circulation and antioxidant, and have cholesterol lowering effects By mixing this excellent nut paste, it is intended to provide a method for producing an antioxidant functional composition with improved flavor and functionality.

The above and other objects and advantages of the present invention will become apparent from the following description of preferred embodiments.

The above object is to obtain a nut mixture obtained by grinding nut raw materials including pecans, walnuts, cashews, cacao nibs and Brazil nuts in a blender; Extruding the nut mixture with an extruder to obtain nut oil and nut solids, respectively; and

Obtaining a nut paste by mixing the nut solids, basil pesto, purified water, and the nut oil in a weight ratio of 2: 0.5-0.7: 0.9-1: 0.3-0.5 and homogenizing them in a homogenizer; A first step comprising;

Preparing sturgeon oil according to the method for obtaining raw materials for sturgeon; Performing a first aging of the sturgeon oil to prepare an aged sturgeon oil; A pre-treatment step of foaming the aged sturgeon oil by using a high-pressure processor and a fine nozzle to form changes in physical properties of the oil; Second aging of the pretreated sturgeon oil aging product; Mixing the aged sturgeon oil and water, in which the second aging has been performed, and introducing the mixture into a distillation extractor and performing a second extraction; After performing the second extraction, water is mixed with the remaining matured sturgeon oil and introduced into a distillation extractor, and third extraction is performed to produce first water-soluble oil; Preparing a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively; and distilling and extracting a mixture of the first water-soluble oil, the first additive composition, and the second additive composition to prepare a second water-soluble oil; a second step of preparing sturgeon water-soluble oil; and

A third step of preparing a food composition by mixing 120 to 150 parts by weight of sturgeon water-soluble oil obtained in the second step and 25 to 35 parts by weight of pumpkin extract with respect to 100 parts by weight of the nut paste obtained in the first step; It can be achieved by a method for producing an antioxidant functional composition comprising.

The method for obtaining the raw material of the sturgeon includes a farming step of culturing the sturgeon fry for 3 years or more using natural antibiotics; A pre-treatment step of shipping the farmed sturgeon and pre-processing the whole fish; A cold storage step of rapidly cooling the pretreated sturgeon, wherein the rapid cooling reaches a temperature of -80 to -20 ° C in 5 to 20 minutes; and thawing and washing the sturgeon stored in the cold, then putting the whole sturgeon into a pneumatic extractor and performing a first extraction to obtain sturgeon oil.

According to the present invention, an antioxidant functional composition containing sturgeon water-soluble oil can be prepared.

The sturgeon water-soluble oil can secure human body stability and environmental friendliness by excluding artificial chemicals such as surfactants and forming a water-soluble oil formulation only by physical separation using natural nanotechnology. In addition, the sturgeon water-soluble oil composition contains sturgeon oil obtained from whole sturgeon, contains a high content of active ingredients in sturgeon, and can secure human safety and improved quality due to non-antibiotics .

According to the present invention, by mixing sturgeon water-soluble oil rich in nutrients such as essential amino acids and excellent in antioxidant and blood circulation improvement effects, and nut paste rich in nutrients such as unsaturated fatty acids and excellent in antioxidant and cholesterol lowering effects, An antioxidant functional composition with improved flavor and functionality can be prepared.

The antioxidant functional composition can be used in various industrial fields, such as, for example, food, drinking water, cosmetics, pharmaceuticals, pet-related products, and vegetable raw material compositions.

However, the effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

1 is a photograph of preparing sturgeon water-soluble oil according to an embodiment of the present invention.
2 and 3 show the results of Experimental Example 1.
4 and 5 show the results of Experimental Example 2.

Hereinafter, the present invention will be described in detail with reference to embodiments and drawings of the present invention. These examples are only presented as examples to explain the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples. .

In addition, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of skill in the art to which this invention belongs, and in case of conflict, this specification including definitions of will take precedence.

In order to clearly explain the proposed invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification. And, when a certain component is said to "include", this means that it may further include other components without excluding other components unless otherwise stated. Also, a “unit” described in the specification means one unit or block that performs a specific function.

In each step, the identification code (first, second, etc.) is used for convenience of description, and the identification code does not describe the order of each step, and each step does not clearly describe a specific order in context. It may be performed differently from the order specified above. That is, each step may be performed in the same order as specified, may be performed substantially simultaneously, or may be performed in the reverse order.

Hereinafter, embodiments and embodiments of the present disclosure will be described in detail with reference to the accompanying drawings. However, it may not be limited to these implementations and examples and figures herein.

One aspect of the present application, a first step of preparing a nut paste; A second step of preparing sturgeon water-soluble oil; And a third step of preparing a mixed composition by mixing 120 to 150 parts by weight of sturgeon water-soluble oil obtained in the second step and 25 to 35 parts by weight of pumpkin extract with respect to 100 parts by weight of the nut paste obtained in the first step; It provides a method for producing an antioxidant functional composition comprising a.

Step 1: Preparation of Nut Paste

First, you can prepare the nuts. The nuts may be, for example, at least one selected from walnuts, almonds, peanuts, cashew nuts, pecans, pistachios, cacao nibs, and Brazil nuts. For example, the nuts may be a mixture of pecans, walnuts, cashews, cacao nibs, and Brazil nuts.

The nuts are excellent in antioxidant activity, lower cholesterol, anti-cancer effect by enhancing immunity, protect brain cells, and are helpful in intake of unsaturated fatty acids and calcium. In addition, due to its unique savory flavor, it can exhibit synergy with the water-soluble sturgeon oil described later in terms of functionality. In particular, it is possible to control the off-flavor and taste peculiar to marine products, and further improve the savory flavor.

Pecans are widely known as ingredients for pies, ice cream, cookies, etc., and pecans contain large amounts of unsaturated fatty acids, calcium, and B vitamins. In particular, it is rich in calcium, which is good for bones, so it is good for preventing osteoporosis in menopausal women, and it is also effective in increasing bone health in growing children and the elderly.

Walnuts are rich in antioxidants and omega-3 fatty acids, which not only help the formation and growth of brain nerve cells, but also play a role in protecting brain cells. In addition, a component called alpha-linolenic acid contained in walnuts has the effect of improving learning ability and memory.

Cashew nuts are rich in vitamin K, pantothenic acid, and linoleic acid, and have micronutrients such as selenium, copper, and magnesium, which are effective for adult diseases by lowering blood cholesterol levels.

Cacao nibs contain polyphenols such as catechin and procyanidin, which are effective in preventing aging, relieving fatigue, and enhancing immunity.

Brazil nuts are extremely high in selenium. Selenium suppresses active oxygen in the body to prevent cell damage caused by active oxygen, and enhances immunity, so it has excellent anti-cancer effects.

When the pecans, walnuts, cashew nuts, cacao nibs, and Brazil nuts are mixed in a weight ratio of 1: 1: 0.8 to 1: 0.5 to 0.6: 0.7 to 0.8, synergy with sturgeon water-soluble oil is the best in terms of sensory properties. can

The nuts are cleaned and prepared, and in some cases, a roasting process performed at a low temperature, for example, at a temperature of 40 to 60 ° C. for 10 to 20 minutes may be performed. When the roasting process is performed, the flavor of the nuts can be further improved.

Then, the nut mixture can be prepared in the form of nut powder by grinding it with a blender or the like or crushing it with a mortar.

Next, it can be prepared by extruding the nut (flour) mixture using a conventional extruder to obtain the oil and fat component and the solid content of the nut, respectively. At this time, the maintenance component can increase the stirring efficiency by stirring while adding little by little when preparing the nut paste later.

As another example, the nut oil component may be mixed with 10 to 20% by weight (relative to the total weight of the nut oil component) of Haejuksun extract as a natural food preservative to prevent oxidation of oil.

Next, a nut paste containing the nut solids may be prepared. The nut paste is an intermediate formulation of solid and liquid, and can be prepared to have sufficient viscosity.

The nut solids may further include basil pesto, purified water, and the nut oil to prepare the nut paste. However, it is not limited thereto, and the nut paste may further include, for example, liquid components such as milk and soymilk, herbs for flavor, and additives commonly used, such as sweeteners for sweetness.

For example, the nut solids, the basil pesto, the purified water, and the nut oil may be mixed in a weight ratio of 2: 0.5 to 0.7: 0.9 to 1: 0.3 to 0.5, put into a homogenizer, and homogenized to form a paste formulation. At this time, the homogenization effect can be further increased by dividing the purified water and the nut oil or nut oil by adding them little by little. In addition, known techniques for forming nut paste formulations may be followed without limitation.

Step 2: Preparation of sturgeon water-soluble oil composition

<Sturgeon Oil>

First, sturgeon oil obtained according to the method for obtaining raw materials for sturgeon can be obtained.

The method for obtaining the raw material of the sturgeon includes a farming step (S1) of culturing the sturgeon fry for 3 years or more using natural antibiotics; A pre-processing step (S2) of shipping the farmed sturgeon and pre-processing them whole; A cold storage step (S3) of rapidly cooling the pretreated sturgeon, such that the rapid cooling reaches a temperature of -80 to -20 ° C within 5 to 20 minutes; and thawing and washing the sturgeon stored in the cold, then putting the whole sturgeon into a pneumatic extractor and performing a first extraction to obtain sturgeon oil (S4).

More specifically, in the step S1, sturgeon, specifically, sturgeon fry is farmed. The breeds of the sturgeon include Beluga sturgeon (Huso huso), Kaluga sturgeon (Huso dauricus), Siberian sturgeon (Acipenser baerii), Russian sturgeon (Acipenser gueldenstaedti), stellet sturgeon (Acipenser ruthenus), white It may be selected from the group consisting of sturgeon (Acipenser transmontanus), Amur sturgeon (Acipenser schrenckii), Chinese sturgeon (Acipenser sinensis), spatula sturgeon (Polyodon spathula) and hybrids thereof. Sturgeon according to an embodiment of the present invention may be, for example, a hybrid of Siberian sturgeon and Beluga sturgeon, but is not limited thereto.

The environment of the aquaculture may be a fresh water farm, and the fresh water source may be river water, ground water, mountain spring water, and tap water, and the fresh water source may be a water source that has undergone purification treatment such as dechlorination treatment with activated carbon.

The farming may be carried out until the sturgeon fry become adult sturgeon weighing at least 1 kg, eg for more than 3 years, eg 3 to 5 years.

The aquaculture is oriented toward an antibiotic-free environment. That is, in the sturgeon farming of the present invention, as in the prior art, chemical components such as various antibiotics and growth promoters administered for weight gain and meat quality improvement of the sturgeon are not used, and natural antibiotics made of natural materials are used. can

For example, bay salt may be used as the natural antibiotic. Specifically, by allowing the sturgeon to consume sea salt while being farmed, impurities including harmful bacteria inside the sturgeon can be effectively removed. Intake of the sun salt is preferably carried out within the range of 1 to 3 times a month.

In the above steps S2 and S3, the cultured sturgeon may be pretreated and then stored in a refrigerator. The pretreatment is performed so that the sturgeon is not cut by part, but in the form of whole fish (separated or uncut whole).

The farmed sturgeon is shipped to perform pretreatment, but first, the sturgeon may be fasted so that it does not consume any food from 10 to 12 days before a predetermined shipment date, for example, 10 days before. By allowing the sturgeon to fast, impurities in the intestines are emptied cleanly, so that the yield of active ingredients of the whole sturgeon can be further increased.

The sturgeon after fasting is shipped out, washed briefly, and then knocked down by hitting the sturgeon head once or twice with a rubber mallet. The stunned sturgeon can then be rapidly cooled directly to carry out storage. The rapid cooling condition may be to reach a temperature of -80 to -20 °C in a short time, for example, between 5 and 20 minutes.

By rapidly cooling the sturgeon after rapidly stunning it in the above-described manner, it is possible to obtain high-quality active ingredients of the whole sturgeon while minimizing the pain of the sturgeon.

In the above step S4, sturgeon raw material, that is, sturgeon oil is obtained.

The cooled sturgeon is thawed for 2 to 6 hours, and then the entire fish body is evenly washed with water or saline to remove various impurities such as residual blood or residual internal organs.

The first extraction may be performed with the sturgeon in whole form. The first extraction method may be performed using a pneumatic extractor, specifically, a pneumatic hot water extractor. The whole sturgeon may be put into the pneumatic extractor together with water as a solvent. At this time, the solvent may further include calamansi undiluted solution, bellflower undiluted solution and beet undiluted solution.

In one example, an extraction raw material obtained by mixing 50% by weight of sturgeon, 41% by weight of water, 5% by weight of calamansi stock solution, 2% by weight of balloon flower stock solution and 2% by weight of beet stock solution is introduced into the pneumatic extractor to prepare 1 extraction can be performed.

The pneumatic extractor may be performed under conditions of 100 to 104 ° C, for example, 102 ° C, 2.3 to 2.7 atm, for example, 2.5 atm, and the extraction time is 98 to 102 hours, for example, 100 hours it is desirable

After the first extraction, specific gravity separation may be performed in which upper, middle, and lower layers are separated using the difference in specific gravity. As a specific method for the specific gravity separation, first, the obtained primary extract is directly put into the stirrer tank without removing steam (kim) while the pneumatic extractor is operating. In this case, the primary extract contained in the agitator tank may be a liquid extract of sturgeon, and the liquid extract of sturgeon may account for about 80% by weight of the total weight of the raw material for extraction.

Thereafter, a secondary extract may be obtained by performing compression of the remaining extraction raw materials once or twice or more with a compressor provided in the pneumatic extractor. In this case, the secondary extract may be sturgeon oil, and the sturgeon oil may account for about 5% by weight with respect to the total weight of the raw material for extraction.

Then, the remaining raw material for extraction, that is, solids of sturgeon can be obtained. The sturgeon solids may include sturgeon meat, cartilage, bone marrow, or a combination thereof. The solid content of the sturgeon may account for about 15% by weight based on the total weight of the raw material for extraction.

The method for obtaining the sturgeon raw material described in step S4 above is to perform extraction and gravity separation at the same time, so that all of the sturgeon liquid extract, sturgeon oil and sturgeon solids obtained from whole sturgeon are obtained in a simpler process. make it possible to obtain

<Sturgeon water-soluble oil composition>

Second, a first aging of the sturgeon oil may be performed to prepare an aged sturgeon oil. Sterilization may be performed before performing the first aging. A method of sterilization may be performed according to known techniques generally used in the art.

The first aging is carried out in a shaded place by putting the sturgeon oil in a container for aging, but may be natural aging performed under conditions similar to natural conditions. Specifically, the aging is preferably performed for 25 days to 35 days, for example, 30 days, for example, at a temperature range of 17 to 21 ° C., for example, 17 ° C., without sunlight and without air. It can be performed under the following conditions.

If the first aging is performed within the above-described condition range, impurities in the sturgeon oil may sink or float and be removed later, thereby increasing the purity of the sturgeon oil and further increasing the content of active ingredients. After the aging, filtration may be performed separately.

Thirdly, the aged sturgeon oil may be pretreated. The pretreatment may be an operation of making the oil state immediately before water solubilization (solubilization) in order to obtain a formulation of water soluble oil later.

A specific method of performing the pretreatment is to cause a change in the physical properties of the sturgeon oil, for example, while passing the sturgeon oil several to tens to hundreds of times through a high-pressure injector and a fine nozzle provided in the high-pressure injector. It could be foaming shark oil. At this time, the efficiency of the foaming operation may be increased while injecting air using a separate injection pump.

This pretreatment process may be repeated a number of times until the oil becomes a formulation like fresh cream. At this time, the point at which the oil becomes a formulation such as whipped cream may be in a state immediately before water-solubilization (solubilization) to obtain water-soluble oil.

This pretreatment process does not use artificial chemical components such as surfactants used in the past, but only physical nanotechnology causes physical property changes in components to form water solubilization (solubilization) to obtain a formulation of water-soluble oil later. make it possible That is, in one embodiment of the present invention, as a method for producing sturgeon water-soluble oil using a natural nanotechnology, in which harmlessness to the human body and environmental friendliness should be given priority, drinking water, food, cosmetics, pharmaceuticals, pet-related fields, and vegetable raw material compositions It can be used in a wide range of fields such as

The pre-treated sturgeon oil-aged product may be stored in a container at room temperature, for example, 1 to 35 ° C, specifically, 18 to 23 ° C, after the second aging. The second aging is natural aging like the first aging, and the specific conditions are the same as the first aging, but the aging period is preferably about 10 days.

Fourth, the first water-soluble oil may be prepared by performing second extraction and third extraction with the pretreated aged sturgeon oil and water. At this time, the method of the second extraction and the third extraction may be distillation extraction.

Specifically, in the second extraction, distillation extraction may be performed after adding the pretreated aged sturgeon oil and water in a 1:1 weight ratio to a distillation extractor. Subsequently, after the second extraction, water vapor is all evaporated and water is mixed with the remaining sturgeon oil in a weight ratio of 1: 1.2 to 1.5, and then third extraction is performed by distillation extraction. The third extraction is repeated at least 30 times, thereby obtaining primary water-soluble oil.

The primary water-soluble oil may be a formulation in the process of preparing a final water-soluble oil. The primary water-soluble oil may be put into a stirrer and stirred once or twice.

Fifthly, an additive composition may be prepared. The additive composition may be a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively.

The primary additive composition may further include at least one selected from the group of primary natural ingredients consisting of Baekbong Ogolgae, white throat mushroom, beet, kelp root, and bellflower. For example, the first additive composition may include a first natural ingredient that is a mixture of edible snails, Baekbong Ogolgae, white throat mushrooms, beets, kelp roots, and bellflowers. At this time, the natural ingredient may be used by washing the raw material cleanly and cutting it into an average size of about 4 to 7 cm.

The first natural ingredient extract and water are mixed at a weight ratio of 1:1 and then put into a pneumatic extractor and extracted for 100 hours at a temperature of 100° C. or higher to prepare a first natural ingredient extract, that is, a first additive composition.

The secondary additive composition is a group of secondary natural ingredients consisting of lotus seeds, sea bamboo shoots, centella asiatica, wormwood, ginseng mugwort, peppermint, yugenpi, hagocho, guccipon, waspong, chaga mushroom, samchae, calamansi and mulberry leaves It may further include one or more selected from. In one example, the secondary additive composition is puerh tea, chrysanthemum, lotus seed, haejuksun, centella asiatica, wormwood, ginseng wormwood, peppermint, yugeunpi, hagocho, guccippong, waspong, chaga mushroom, samchae, calamansi and mulberry leaves It may contain secondary natural ingredients that are mixtures. At this time, the natural ingredient may be used by washing the raw material cleanly and cutting it into an average size of about 4 to 7 cm.

After mixing the secondary natural component and water at a weight ratio of 1:1, it is introduced into a pneumatic extractor and extracted for 100 hours at a temperature of 100° C. or higher to prepare a secondary natural component extract, that is, a secondary additive composition.

Then, the primary natural component extract and the secondary natural component extract were aged at room temperature, 1 to 35 ° C, for example, 19 to 23 ° C, for 10 days, and then put together in a stirrer and stirred, Distillation extraction can be performed. For example, 50 parts by weight of the secondary natural ingredient extract and 50 parts by weight of water may be mixed with 100 parts by weight of the first natural ingredient extract, and then put into a distillation extractor for distillation and extraction.

When the distillation extraction is completed, a mixed additive composition obtained from the mixed extraction of the first natural component extract and the second natural component extract can be obtained.

Sixth, a secondary water-soluble oil may be prepared by distilling and extracting the primary water-soluble oil and the mixed additive composition. The primary water-soluble oil and the mixed additive composition may be mixed in a weight ratio of 1:1, and the distillation extraction may be performed once or two to three times.

The secondary water-soluble oil may be the “sturgeon water-soluble oil composition” in the present invention. That is, the sturgeon water-soluble oil composition may include a mixed additive composition obtained by distilling and extracting a mixture of sturgeon oil and natural additives.

The sturgeon water-soluble oil (composition) is an oil formulation that can be easily solubilized in water without artificial chemicals such as surfactants, and can be widely used in drinking water or food fields, where harmlessness to the human body and environmental friendliness must be given priority. .

Step 3: Preparation of antioxidant functional composition

A mixed composition may be prepared by mixing 120 to 150 parts by weight, for example, 130 parts by weight of the sturgeon water-soluble oil (composition) obtained in the second step, with respect to 100 parts by weight of the nut paste obtained in the first step.

The mixed composition may further include 25 to 35 parts by weight of pumpkin (old pumpkin) extract, for example, 30 parts by weight, based on 100 parts by weight of nut paste, to improve the sensory properties.

The pumpkin extract may play a role in allowing the nut paste and the sturgeon water-soluble oil to taste well together. The preparation method of the pumpkin extract may follow a known technique.

The nut paste, the sturgeon water-soluble oil composition, and the pumpkin extract may be mixed more uniformly by stirring in a conventional manner.

The stirring is sufficiently performed at a speed of 150 to 200 rpm for 30 minutes to 90 minutes, and at this time, a natural emulsifier such as lecithin may be included so that the mixture is more uniformly mixed.

The composition can be used in various industrial fields, such as, for example, food, drinking water, cosmetics, pharmaceuticals, pet-related products, and vegetable raw material compositions.

For example, the composition may be prepared in the form of a food composition, that is, various health supplements provided by conventional methods in the art. For example, the food composition, liquid phase, such as suspension, emulsion, beverage; or solids such as sticks, gels, pills, tablets, jellies, and chips; It can be produced in a variety of formulations.

As an example, the food composition may be prepared as a beverage composition, and the shape may be, for example, liquid stick type, liquid pouch type, etc., but is not limited thereto.

When the food composition is prepared as a beverage composition, in addition to the above-described components, for example, acacia honey, pear juice, bellflower juice, apple juice, or a combination thereof may further include natural sweetener components. As an example, 15 to 20 parts by weight of a natural sweetener obtained by mixing pear juice and acacia honey in a weight ratio of 1: 1 may be further included with respect to 100 parts by weight of the natural raw juice mixture.

By further including these natural sweetening ingredients, it is possible to manufacture a new health functional food that can simultaneously secure the nutritional components of sturgeon and the antioxidant function of natural juice by improving the sensory by eliminating the reluctance to eat sturgeon. there is.

The sturgeon water-soluble oil composition according to an embodiment of the present invention includes sturgeon oil obtained from whole sturgeon, contains a high content of active ingredients in sturgeon, and improves human safety and quality due to non-antibiotics. can be obtained. In addition, human body stability and environmental friendliness can be secured by excluding artificial chemicals such as surfactants using a physical natural nanotechnology and forming a water-soluble oil formulation using only a physical separation method.

상기 표 2와 같이, 96 well plate에 각 농도별 시험군을 100 ㎕씩 분주한 뒤, 24 시간 동안 반응시킨 ABTS 시약을 a,b에 100 ㎕씩 첨가, a', b' 에는 증류수 100 ㎕를 첨가하여 30분간 반응시켰다.
그 다음에, 700nm 파장에서 흡광도를 측정하였다. 그런 다음, ABTS 프리 라디칼 소거능(%)을 하기 식 1에 따라 산출하였다. 실험 결과를 도 2 및 도 3에 나타내었다.
<식 1>

도 2 및 도 3을 참조하면, 3.125%, 6.25%, 12.5%, 25%, 50 %의 각 농도별 시험군들은 각각 3.6 ± 0.9 %, 4.4 ± 0.8 %, 8.4 ± 0.8 %, 15.5 ± 1.3 %, 26.4 ± 0.8 %의 ABTS free radical 소거능을 보였다.
이로써, 실시예 1에 따른 철갑상어 추출물, 즉, 철갑상어 오일 조성물은, 프리 라디칼 소거에 의한 항산화 활성 효능이 있음을 확인할 수 있었다.
<실험예 2 : 소거 평가 시험 (2) >
실시예 1에 따라 제조된 철갑상어 수용성 오일에 대한 항산화 기능을 측정하기 위하여, DPPH 프리 라디칼 소거 평가 시험(DPPH free radical scavenging assay)을 실시하였다. (DPPH : 2,2-diphenyl-1-picrylhydrazy)
구체적인 실험방법은 다음과 같다.
실시예 1의 철갑상어 수용성 오일을 버퍼(PBS)로 처리하여 5개의 농도(3.125%, 6.25%, 12.5%, 25%, 50%)로 각 시험군들을 제조하였다. 정확한 비교를 위하여, 대조군(control, 에탄올)과 양성 대조군(ascorbic acid)(0.00625%, 0.0125%, 0.025%, 0.05%, 0.1%)도 함께 설정하였다. 또한, 상기 시험군들에 대하여 각각 DPPH 처리군(a, b)과 미처리군(a', b')으로 분류하였다. 상기한 실험 샘플들에 대하여 하기 표 3 및 표 4에 나타내었다.

상기 표 4와 같이, 96 well plate에 각 농도별 시험군을 100 ㎕씩 분주한 뒤, 24 시간 동안 반응시킨 DPPH 시약을 a,b에 100 ㎕씩 첨가, a', b' 에는 증류수 100 ㎕를 첨가하여 30분간 반응시켰다.
그 다음에, 517nm 파장에서 흡광도를 측정하였다. 그런 다음, DPPH 프리 라디칼 소거능(%)을 하기 식 2에 따라 산출하였다. 실험 결과를 도 4 및 도 5에 나타내었다.
<식 2>

도 4 및 도 5를 참조하면, 3.125%, 6.25%, 12.5%, 25%, 50 %의 각 농도별 시험군들은 각각 각 농 도에서 12.4 ± 0.7 %, 12.6 ± 1.2 %, 17.8 ± 1.6 %, 23.9 ± 0.9 %, 34.3 ± 2.0 %의 DPPH free radical 소거능을 보였다.
이로써, 실시예 1에 따른 철갑상어 추출물, 즉, 철갑상어 오일 조성물은, 프리 라디칼 소거에 의한 항산화 활성 효능이 있음을 확인할 수 있었다.Furthermore, the mixed composition according to an embodiment of the present invention provides an abundance of nutrients such as low-molecular-weight collagen, protein, essential amino acids, and vitamins from the sturgeon water-soluble oil composition, and various nutrients and By allowing various antioxidant functions to exert synergy together, the antioxidant effect can be further increased.
Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for explaining the present invention in more detail, and the scope of the present invention is not limited to these examples.
[Example 1]
Manufacture of sturgeon raw materials
Farming with eco-friendly non-antibiotics for more than 3 years, feeding clean sturgeon (Siberian sturgeon) with bay salt at least once a month, fasting for 10 days according to the shipping time, and then hitting the head with a rubber mallet once or twice The samples were stunned without pain and immediately quenched and stored. After washing the rapidly frozen sturgeon, the entire sturgeon is put in a pneumatic hot water extractor with 50% sturgeon, 41% water, 5% calamansi undiluted solution, 2% bellflower seed undiluted solution, and 2% beet undiluted solution at 102℃ and 2.5 atm. After 100 hours of extraction, the mixture was separated at the upper, middle, and lower specific gravity points to obtain 80% sturgeon liquid extract, 15% sturgeon solid content, and 5% sturgeon oil, respectively.
Preparation of sturgeon water soluble oil
The obtained sturgeon oil was aged at 17° C. for more than 30 days and then passed through a fine nozzle using a high-pressure pump (high-pressure sprayer) repeatedly dozens of times to foam like fresh cream. This is an operation that causes a change in physical properties to bring it to a state before water-solubility, and after this is completed, it is stored at room temperature for natural aging for another 10 days.
Aged sturgeon oil and water, which have been subjected to the physical natural separation method described above, are mixed in equal proportions (50% aged sturgeon oil + 50% water), put into a distillation extractor, extracted, and returned to the remaining aged sturgeon oil. Only water was added at 120% and extracted by distillation more than 30 times to prepare a first water-soluble oil.
On the other hand, a primary additive was prepared by extracting 50% + 50% water of a mixture of edible snails, Baekbong Ogolgae, white neck mushrooms, beets, kelp roots, and bellflower seeds in a pneumatic hot water extractor for 100 hours. In addition, 50% of a mixture of puerh tea, chrysanthemum, lotus seed, sea bamboo shoot, centella asiatica, wormwood, wormwood, peppermint, yugenpi, hagocho, guccipon, waspong, chaga mushroom, samchae, calamansi and mulberry leaves and water 50% was put into a pneumatic hot water extractor to prepare a secondary additive extracted for 100 hours, and then stirred in a stirrer together with the primary additive.
Then, based on 100 parts by weight of the first additive, 50 parts by weight of the second additive and 50 parts by weight of the first water-soluble oil were placed in a distillation extractor and distilled and extracted to prepare final sturgeon water-soluble oil.
Referring to Figure 1, it can be seen that the sturgeon water-soluble oil produced by the above-described method has a transparent appearance.
<Experimental Example 1: Erasure Evaluation Test (1)>
In order to measure the antioxidant function of the water-soluble sturgeon oil prepared in Example 1, an ABTS free radical scavenging assay was performed. (ABTS: 2,2'-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)
The specific test method is as follows.
Each test group was prepared at five concentrations (3.125%, 6.25%, 12.5%, 25%, and 50%) by treating the water-soluble sturgeon oil of Example 1 with a buffer (PBS). For accurate comparison, a control (control, ethanol) and a positive control (Trolox) (0.00625%, 0.0125%, 0.025%, 0.05%, 0.1%) were also set together. In addition, the test groups were classified into ABTS treated groups (a, b) and untreated groups (a', b'), respectively. The above experimental samples are shown in Tables 1 and 2 below.

As shown in Table 2 above, after dispensing 100 μl of each test group for each concentration in a 96-well plate, 100 μl of ABTS reagent reacted for 24 hours was added to a and b, and 100 μl of distilled water was added to a' and b' was added and reacted for 30 minutes.
Then, absorbance was measured at a wavelength of 700 nm. Then, the ABTS free radical scavenging ability (%) was calculated according to Equation 1 below. Experimental results are shown in FIGS. 2 and 3 .
<Equation 1>

2 and 3, the test groups for each concentration of 3.125%, 6.25%, 12.5%, 25%, and 50% were 3.6 ± 0.9%, 4.4 ± 0.8%, 8.4 ± 0.8%, and 15.5 ± 1.3%, respectively. , showed ABTS free radical scavenging ability of 26.4 ± 0.8%.
Thus, it was confirmed that the sturgeon extract, that is, the sturgeon oil composition according to Example 1, has an antioxidant activity effect by scavenging free radicals.
<Experimental Example 2: Erasure Evaluation Test (2)>
In order to measure the antioxidant function of the water-soluble sturgeon oil prepared in Example 1, a DPPH free radical scavenging assay was performed. (DPPH: 2,2-diphenyl-1-picrylhydrazy)
The specific test method is as follows.
Each test group was prepared at 5 concentrations (3.125%, 6.25%, 12.5%, 25%, 50%) by treating the water-soluble sturgeon oil of Example 1 with a buffer (PBS). For accurate comparison, a control (ethanol) and a positive control (ascorbic acid) (0.00625%, 0.0125%, 0.025%, 0.05%, 0.1%) were also set together. In addition, the test groups were classified into DPPH-treated groups (a, b) and untreated groups (a', b'), respectively. The above experimental samples are shown in Tables 3 and 4 below.

As shown in Table 4 above, after dispensing 100 μl of each test group for each concentration in a 96-well plate, 100 μl of DPPH reagent reacted for 24 hours was added to a and b, and 100 μl of distilled water was added to a' and b' was added and reacted for 30 minutes.
Then, absorbance was measured at a wavelength of 517 nm. Then, the DPPH free radical scavenging ability (%) was calculated according to the following formula 2. Experimental results are shown in FIGS. 4 and 5 .
<Equation 2>

4 and 5, the test groups for each concentration of 3.125%, 6.25%, 12.5%, 25%, and 50% were 12.4 ± 0.7%, 12.6 ± 1.2%, 17.8 ± 1.6%, DPPH free radical scavenging activity of 23.9 ± 0.9 % and 34.3 ± 2.0 % was shown.
Thus, it was confirmed that the sturgeon extract, that is, the sturgeon oil composition according to Example 1, has an antioxidant activity effect by scavenging free radicals.

In this specification, only a few examples of various embodiments performed by the present inventors are described, but the technical spirit of the present invention is not limited or limited thereto, and can be modified and implemented in various ways by those skilled in the art, of course.

Claims (2)

Obtaining a nut mixture obtained by grinding nut raw materials including pecans, walnuts, cashews, cacao nibs and Brazil nuts in a blender;
Extruding the nut mixture with an extruder to obtain nut oil and nut solids, respectively; and
Obtaining a nut paste by mixing the nut solids, basil pesto, purified water, and the nut oil in a weight ratio of 2: 0.5-0.7: 0.9-1: 0.3-0.5 and homogenizing them in a homogenizer; A first step comprising;
Preparing sturgeon oil according to the method for obtaining raw materials for sturgeon;
Performing a first aging of the sturgeon oil to prepare an aged sturgeon oil;
A pre-treatment step of foaming the aged sturgeon oil by using a high-pressure processor and a fine nozzle to form changes in physical properties of the oil;
Second aging of the pretreated sturgeon oil aging product;
Mixing the aged sturgeon oil and water, in which the second aging has been performed, and introducing the mixture into a distillation extractor and performing a second extraction;
After performing the second extraction, water is mixed with the remaining matured sturgeon oil and introduced into a distillation extractor, and third extraction is performed to produce first water-soluble oil;
Preparing a first additive composition containing edible snails and a second additive composition containing Puer tea and chrysanthemum, respectively; and
A second step of preparing water-soluble sturgeon oil, comprising distilling and extracting a mixture of the first water-soluble oil, the first additive composition, and the second additive composition to produce a second water-soluble oil; and
A third step of preparing a mixed composition in which 120 to 150 parts by weight of sturgeon water-soluble oil obtained in the second step and 25 to 35 parts by weight of pumpkin extract are mixed with 100 parts by weight of the nut paste obtained in the first step; A method for producing an antioxidant functional composition comprising: According to claim 1,
The method for obtaining the raw material of the sturgeon is,
A farming step in which sturgeon fry are farmed for at least 3 years using natural antibiotics;
A pre-treatment step of shipping the farmed sturgeon and pre-processing the whole fish;
A cold storage step of rapidly cooling the pretreated sturgeon, wherein the rapid cooling reaches a temperature of -80 to -20 ° C in 5 to 20 minutes; and
A method for producing an antioxidant functional composition comprising the steps of thawing and washing the sturgeon stored in a refrigerator, then putting the whole sturgeon into a pneumatic extractor and performing a first extraction to obtain sturgeon oil.

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