KR102466606B1 - A herbal air freshener for a dog aromatherapy - Google Patents

Abstract

The present invention relates to a herbal fragrance for companion dogs. More specifically, it relates to herbal fragrances for dogs, including sancho, nutmeg, and zillaja as herbal medicines, and a manufacturing method thereof. Since the oriental fragrance for dogs according to the present invention is a scent emitted by steering natural herbal medicine and essential oil, it can solve the problems of synthetic fragrances. In addition, there is no rejection of the companion dog for the fragrance of the air freshener, and it suppresses the occurrence of mold in a room temperature and high humidity environment and has an effect of removing odors in the house.

Description

A herbal air freshener for a dog aromatherapy}

The present invention relates to a herbal fragrance for companion dogs. More specifically, it relates to herbal fragrances for dogs, including sancho, nutmeg, and zillaja as herbal medicines, and a manufacturing method thereof.

Today, with many people forming nuclear families and the proportion of people living alone increasing, the number of households with companion animals is increasing. As a result, the companion animal market is growing rapidly.

Companion dogs account for the largest share among companion animals, and their population is rapidly increasing. Air fresheners currently on the market for companion dogs are generally artificially scented and contain fragrances that are not liked by dogs or are fatal to dogs. On the other hand, various chemical components may cause adverse effects on the bronchus or skin.

In a dictionary sense, air freshener refers to drugs having a good scent, and removes or replaces odors or disgusting odors in living spaces such as indoors such as rooms and offices, public toilets, and interiors of cars. And it is used for the purpose of obtaining a sense of cleanliness or stability through fragrance. In recent years, quality of life is considered important, and the range of use for stress relief or therapeutic effect has been expanded.

In the case of existing synthetic fragrances, the fragrance is excellent at the beginning, but the fragrance decreases over time, resulting in a short product lifespan. There are concerns about side effects such as nervousness, hallucinations, anesthesia, muscle infarction, lethargy, and cranial nerve infarction. In the case of methanol, one of the components of some synthetic fragrance products, it is oxidized to formaldehyde, a toxic substance in the human body. Since the human body does not have an enzyme that can decompose formaldehyde, it affects the central nervous system and damages the nerves. It can cause eye blindness, tinnitus, digestive problems and loss of sensation. In addition, isopropanol, one of the other ingredients, can cause headaches or dizziness and can also accumulate in the body.

In addition, LAS (linear alkylbenze sulphonate), AEO (fatty alcohol-polyoxyethylene ether), and betaine among the synthetic surfactants used in fragrances containing oil components have high irritation in skin irritation evaluation, and are It has also been reported to be harmful to humans. In addition, synthetic surfactants other than LAS, AEO, and Betaine can also stimulate the scalp or skin, and when adsorbed and accumulated in the body, they remain in the liver and blood, causing cancer, dermatitis such as atopy, or allergy.

On the other hand, dogs have a sense of smell that is about 10,000 times more sensitive than humans, and people who live with dogs need air fresheners tailored to dogs. Most of the air fresheners on the market are made of chemicals. Accordingly, attempts have been made to prepare fragrances using herbal medicines rather than synthetic fragrances. As an example, Korean Patent Registration No. 10-1175656 contains 20 to 35% by weight of raw rice, 20 to 35% by weight of dermis, 10 to 15% by weight of octagonal incense, 10 to 15% by weight of cinnamon, 10 to 15% by weight of cloves, Disclosed is a herbal fragrance composition containing 5 to 8% by weight of mint, 5 to 8% by weight of Angelica guinea pig, 2 to 3% by weight of Yongnoe, and 3 to 5% by weight of Cnidium.

Herbal medicines, which are natural resources, have the advantage of being relatively clear about the pharmacological components of herbal medicines as well as safety in that distribution and manufacturing management are relatively thorough with GMP certification compared to other natural products. Therefore, attempts are being made to devise air fresheners that dogs and owners can enjoy together by using natural products and essential oils extracted from herbal medicines.

In addition, most of the air freshener products introduced at the fair related to companion animal products are mostly composed of natural ingredients with minimal toxicity, and most of the air fresheners have fragrances that dogs do not object to.

Under this background, the present inventors have made research efforts to develop a fragrance that can be used together with dogs and companions by using aromatic herbal medicines in accordance with the trend of the companion animal-related product market. The present invention was completed by devising a fragrance that the companion can enjoy together.

Specifically, the present inventors use herbal medicines in their natural state as a composition to maintain a fresh fragrance for a long time and have a mild fragrance, and based on an oriental fragrance with sterilization and odor removal functions, but various side effects of existing synthetic fragrances on the human body, It was intended to compensate for problems such as a slight scent diffusion effect and a short duration of scent. In particular, alcohol is used instead of synthetic surfactants to combine hydrosol, a herbal medicine extract extracted through a water-cooled circulation type extractor, with essential oils, such as eucalyptus oil and neroli oil, to help remove odors from dogs and purify the air in the room. We have developed an herbal air freshener for dogs that is not burdensome in terms of smell and that the scent spreads well in the room, and both dogs and owners can use it with satisfaction.

KR 10-1175656 B

An object of the present invention is to provide a herbal fragrance for dogs, including Chinese pepper, nutmeg, and Zillaja as herbal medicines, and a method for manufacturing the same.

In order to achieve the above object, the present invention provides a herbal fragrance for companion dogs containing sancho, nutmeg and zillaja as herbal medicines.

In addition, the present invention comprises the steps of a) obtaining a hydrosol of Sancho, Nutmeg and Zillaeja each as a herbal medicine; b) preparing a hydrosol-type herbal medicine base by adding 0.1 to 3 parts by weight of nutmeg hydrosol and 0.1 to 3 parts by weight of Zillaja hydrosol based on 1 part by weight of Sancho hydrosol obtained above; c) preparing a fragrance base by adding vodka or alcohol as a natural surfactant to the herbal medicine base in the same ratio; and d) adding 0.5 to 1.5 parts by weight of an essential oil blended with eucalyptus oil and neroli oil based on 100 parts by weight of the prepared fragrance base.

Since the herbal fragrance for dogs according to the present invention is a fragrance emitted from hydrosol and essential oil, which are herbal medicine extracts, it can solve the problems of synthetic fragrances and has excellent odor (fragrance), freshness index, and fragrance durability. In addition, it is possible to remove odors, mites, or germs from dogs, and it can be used safely because it does not cause mold or deterioration even after a long time at room temperature.

In addition, the oriental fragrance for dogs according to the present invention inhibits the occurrence of mold even at room temperature and in a high-humidity environment, so it can be used for a long time while maintaining the same scent at any time regardless of the season, providing an optimal environment. It has excellent antibacterial effect.

1 is a diagram showing the results of a sensory test for the degree of smell (fragrance) of an herbal air freshener for dogs according to an embodiment of the present invention.
2 is a diagram showing the results of a sensory test for the degree of fragrance persistence of an herbal air freshener for dogs according to an embodiment of the present invention.
3 is a diagram showing the results of a sensory test for the freshness index (human) degree of an herbal air freshener for dogs according to an embodiment of the present invention.
4 is a diagram showing the results of a sensory test on the degree of diffusion of the fragrance of an herbal air freshener for dogs according to an embodiment of the present invention.
5 is a diagram showing the results of a sensory test for the degree of liking or disliking of an herbal air freshener for dogs according to an embodiment of the present invention.
6 is a view showing the results of a sensory test for the degree of help of the herbal air freshener for dogs according to an embodiment of the present invention in removing the dog's odor.
7 is a diagram showing the results of a sensory test for the degree of purchase intention of an herbal air freshener for dogs according to an embodiment of the present invention.
8 is a diagram showing the MIC (minimum inhibitory concentration) results for the standard super bacteria strain of the oriental fragrance for dogs according to an embodiment of the present invention.
9 is a diagram showing the MIC (minimum inhibitory concentration) results of ampicillin and oxacillin, which were used as control groups for comparison of antibacterial effects of oriental fragrances for dogs according to an embodiment of the present invention, for superbacterial standard strains.

The present invention relates to a herbal fragrance for companion dogs. In detail, it relates to a distillate extracted from herbal medicines that are not toxic to dogs, that is, a herbal fragrance for dogs including Chinese pepper, nutmeg, and Chinese pepper hydrosol, alcohol and essential oil, and a method for manufacturing the same.

As one aspect, the present invention provides a herbal fragrance for dogs, including sancho, nutmeg and zillajaja as herbal medicines.

The term "sancho" refers to a medicinal material made by removing seeds as much as possible as the rind of a cortaceae plant or the rind of a plant of the same genus. It warms the stomach to get rid of cold, has the effect of dehumidifying pain, eliminates pests, stops itching and diarrhea, and treats poisoning symptoms caused by fish and shellfish.

The term "nutmeg" refers to a well-ripe seed of the nutmeg family and a medicinal material from which the seed husk is removed. It also acts as an aromatic stomach acid, and acts as a carminative, geonwi, anti-cancer, anti-inflammatory, hepatocellular protection, and antihyperlipidemia.

The term "getting sick" refers to the fruit of Namgasae of the Namgasae family, and is used as a diuretic, anti-inflammatory, and blood-purifying medicine for headaches and eye diseases, and is also used as an external medicine, lowering blood pressure, diuretic, anthelmintic, improving vitiligo, protecting liver cells, and soothing. , antifungal, anti-hypersensitivity action.

The herbal medicine is not limited thereto, but may include 0.1 to 3 parts by weight of nutmeg and 0.1 to 3 parts by weight of Chinese pepper based on 1 part by weight of Chinese pepper. Preferably, it may be included in 0.5 to 2 parts by weight of nutmeg and 0.5 to 2 parts by weight of pepper based on 1 part by weight of Japanese pepper. More preferably, it may be included in 0.5 parts by weight of nutmeg and 0.5 parts by weight of peppercorns based on 1 part by weight of Japanese pepper.

The herbal medicine is not limited thereto, but may be extracted in the form of a hydrosol using a water-cooled cooling circulation type extractor.

The term "hydrosol" means a colloidal solution using water as a dispersion medium. Hydrosol, also referred to as floral water, is a water-soluble component of plants that comes out when essential oils are extracted. Hydrosol is non-irritating and acts mildly on the body, so it can be safely used even for young animals. It is weakly acidic, similar to the skin of most animals, and balances the pH of the skin and creates a skin environment in which bacteria do not proliferate.

The hydrosol is extracted using a water-cooled cooling circulation type extractor, and its manufacturing process is the same as the aroma distillation process for extracting essential oil. Most hydrosols are made by water or steam distillation.

The herbal fragrance for dogs may further include a natural surfactant and essential oil.

The natural surfactant refers to a surfactant other than an artificially synthesized surfactant, and is used to uniformly mix a hydrosol type herbal medicine base and essential oil.

The natural surfactant may be, but is not limited to, vodka or alcohol, and the vodka or alcohol is characterized in that the alcohol content is 40%.

The natural surfactant is not limited thereto, but may be mixed with the herbal medicine base in hydrosol form in the same ratio.

In the present invention, the term "herbal herb base" is a basic herbal material for preparing a herbal fragrance for dogs, and includes 0.1 to 3 parts by weight of nutmeg hydrosol and 0.1 to 3 parts by weight of ginseng hydrosol based on 1 part by weight of Japanese pepper hydrosol. raw material added.

In addition, the essential oil may be composed of eucalyptus oil and neroli oil.

The essential oil may be added in an amount of 0.5 to 1.5 parts by weight, but is not limited thereto, based on 100 parts by weight of a fragrance base in which a hydrosol type herbal medicine base and a natural surfactant are mixed in equal proportions.

In the present invention, the term "fragrance base" is a basic fragrance raw material for preparing a herbal fragrance for dogs, and is a raw material in which the herbal medicine base and a natural surfactant are mixed in the same ratio.

In one embodiment of the present invention, sancho, nutmeg, and ginseng hydrosol are mixed in a weight ratio of 2: 1: 1 to prepare a hydrosol-type herbal medicine base, and vodka (which serves as the herbal medicine base and natural surfactant) 40% alcohol) was mixed in a weight ratio of 1: 1 to prepare a fragrance base. Herbal fragrance for dogs was finally prepared by adding essential oil prepared by blending 0.6 mL of eucalyptus oil and 0.6 mL of neroli oil to 100 mL of the fragrance base mixed with herbal medicine base and natural surfactant (Examples 1 to 5 ).

The oriental fragrance for dogs of the present invention is characterized by having an antibacterial effect. Specifically, the fragrance is a fragrance using Chinese herbal medicines such as sancho, nutmeg, and ginseng, and natural surfactants such as vodka or alcohol, and eucalyptus oil and neroli oil, which are scents preferred by dogs, and can be safely used without a dog's reluctance. It is characterized in that it is a harmless herbal fragrance for dogs.

The oriental air freshener for companion dogs of the present invention has a good fragrance and excellent fragrance durability, and thus can improve the quality of life in a living space. In addition, it gives a refreshing feeling to dogs and people who live with them without feeling repulsive, and has excellent antibacterial effects as well as antiseptic effects.

The oriental air freshener for companion dogs according to the present invention can be used sufficiently in a normal living room because the degree of diffusion of fragrance is made up to a distance of about 4 m. Therefore, the oriental fragrance for dogs according to the present invention is not limited thereto, but is preferably used in a state located within 1 m to 4 m from the living room or room.

As another aspect, the present invention is a) obtaining a hydrosol of sancho, nutmeg and zillaeja each as a herbal medicine; b) preparing a hydrosol-type herbal medicine base by adding 0.1 to 3 parts by weight of nutmeg hydrosol and 0.1 to 3 parts by weight of Zillaja hydrosol based on 1 part by weight of Sancho hydrosol obtained above; c) preparing a fragrance base by adding vodka or alcohol as a natural surfactant to the herbal medicine base in the same ratio; and d) adding 0.5 to 1.5 parts by weight of essential oil blended with eucalyptus oil and neroli oil based on 100 parts by weight of the prepared fragrance base. .

The step a) is a step of obtaining hydrosols of Chinese pepper, nutmeg, and Chinese pepper as herbal medicines, and each hydrosol of Japanese pepper, nutmeg, and Chinese pepper can be extracted using a water-cooled cooling circulation type extractor.

Step b) is a step of preparing a hydrosol-type herbal medicine base, wherein the herbal medicine base contains 0.1 to 3 parts by weight of nutmeg hydrosol and 0.1 to 3 parts by weight of nutmeg hydrosol based on 1 part by weight of Sancho hydrosol obtained in step a). It can be prepared by adding 3 parts by weight.

Preferably, the herbal medicine base may be prepared by adding 0.5 to 2 parts by weight of nutmeg and 0.5 to 2 parts by weight of Chinese pepper based on 1 part by weight of Chinese pepper, more preferably 0.5 parts by weight of nutmeg based on 1 part by weight of Japanese pepper, and It can be prepared by adding 0.5 parts by weight of ginseng.

Step c) is a step of preparing a fragrance base, which may be prepared by adding a natural surfactant to the herbal medicine base prepared in step b) in the same ratio.

The natural surfactant may be vodka or alcohol, and the vodka or alcohol may have an alcohol content of 40%, but is not limited thereto.

Step d) is a step of adding essential oil to the fragrance base prepared in step c), and 0.5 to 1.5 parts by weight of the essential oil may be added based on 100 parts by weight of the fragrance base.

The essential oil may be prepared by blending eucalyptus oil and neroli oil.

The oriental fragrance for dogs of the present invention prepared by the above manufacturing method is composed of only natural materials, not chemicals, and is characterized in that it is manufactured by excluding harmful chemicals as much as possible.

In addition, the herbal air freshener for dogs has an excellent antibacterial effect, so it can be used for a long time without a separate preservative, and it not only removes the dog's odor, but also purifies the indoor air to improve the quality of living for dogs and companions. has

On the other hand, the herbal air freshener for dogs uses herbal medicines, specifically Chinese pepper, nutmeg, and hydrosol of japonica as the raw material of the fragrance, and has fragrance properties that are harmless to the human body. It has the characteristics of a safe air freshener that can be used by dogs without objection to the scent.

The present invention will be described in more detail through the following examples. These examples are only for specifically explaining the present invention, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Example 1: Hydrosol-based sensory test

For the manufacture of herbal air fresheners for dogs, the herbal medicines used in the preparation of this base were purchased from the Dongbu Herbal Medicines Domestic Herbal Medicine General Store (Dbubu Herbal Medicine Farming Association, 1679 Yeosun-ro, Haeryong-myeon, Suncheon-si, Jeollanam-do). After purchase, it was extracted with hydrosol immediately after opening. In addition, the essential oil used in the preparation of this base was purchased from Herb Aehyang (http://herb aehyang.com/), and was sealed immediately after use to prevent loss of essential oil components. On the other hand, the diffuser container and fiber sticks necessary for sample production were purchased from Star Candle (http://www.star-candle.com/), and the fiber sticks were cut in half and used.

For this manufacturing process, after testing the taste of 130 or so initially extracted medicinal materials, various extracts were combined according to the theory of oriental medicine. Of these, seven bases were made and blind tests were conducted twice.

In the first irradiation, as shown in Table 1 below, 7 types of bases were steered at each ratio and placed in a microtube. Thereafter, the preference of fragrance through the sense of smell was measured in a space about 30 cm away from each of these hydrosol combinations. At this time, the fragrance preference measurement was performed in a closed space of 5 pyeong for a total of 16 people. The results are shown in Table 1, and it was confirmed that base No. 6 (Nutmeg: Jilleoja: Sancho = 1: 1: 1) was highly preferred.

As a result of a blind test, it was confirmed that base No. 6 containing Sancho extract was highly preferred, so Sancho-based bases (Sancho, Zillaja, Nutmeg) were made.

In order to extract the hydrosol base of nutmeg, Chinese pepper, and Chinese pepper, herbal medicines were extracted using a water-cooled cooling circulation type extractor, respectively. Extraction information is shown in Table 2 below.

Raw material origin weight (kg) menstruum Solvent usage (mL) collection time Acquisition (cc) nutmeg Indonesia 1.2 Distilled water 10,500 2 hours 30 minutes 3,900 let's get tired China 1.2 Distilled water 10,500 2 hours 30 minutes 3,800 Sancho China 2.4 Distilled water 19,500 2 hours 30 minutes 5,100

The hydrosol ratio of sancho, nutmeg, and zillaja extracted through the above method was varied and contained in a micro tube. Thereafter, as a secondary investigation, the preference for fragrance through the sense of smell was measured in a space about 30 cm away from each of these hydrosol combinations, and the results are shown in Table 3 below. At this time, the fragrance preference measurement was performed in a closed space of 5 pyeong for a total of 16 people, and after tasting, they were asked to check two of their favorite fragrances. The measured results were converted to a scale of 100, and the results are shown in Table 4 below.

On the other hand, as can be seen in Table 1 above, when herbal medicine hydrosols are combined and tested in 7 ways, No. 5 hydrosol base (Angelica: Deukryangman rose pink: dermis: broiler = 1:1:1:1) showed the highest preference. However, due to the nature of herbal air fresheners that can be used while living with dogs, 'broiled chicken', which can cause skin irritation when in contact with a dog's skin, was excluded.

Accordingly, hydrosol No. 6, which is the most preferred hydrosol base except for the hydrosol base in which 'broiler' was used, was used as the base of the fragrance.

In addition, as can be seen in Tables 3 and 4 above, No. ④, consisting of a ratio of pepper: nutmeg: jjilleoja = 2: 1: 1, showed the highest preference, followed by pepper: nutmeg: jjilleo Ja = 1:1:1, Sancho:Nutmeg:Zillaeja = 1:2:2 showed high preference.

Example 2: Investigation and Selection of Essential Oil Preference

As a result of Example 1, the preference for the hydrosol base ④, which was found to be highly preferred in Table 4, was investigated when it was steered with essential oil. Specifically, the dog's favorite essential oil was steered into the hydrosol base ④, and the preference of the scent through the sense of smell was measured at a distance of 30 cm from the steered sample. At this time, the measurement of preference for fragrance was performed in a closed space of 10 pyeong for a total of 20 people. As shown in Table 5, the degree of preference was measured as a score from 1 to 10, and the measured result was converted. The conversion results are shown in Table 6 below.

However, the essential oils were abbreviated as follows in Table 5. Here, PA is patchouli, TH is cedarwood, RC is Roman chamomile, and NE is neroli. Also, ZE is geranium, MJ is marjoram, LA is lavender, OR is sweet orange, and EU is eucalyptus.

Steering preference result of selected hydrosol base and essential oil (%) oil PA TH RC NE ZE MJ LA OR EU preference 28 8.5 38.5 65.5 46 55 58 55 61.5

As a result of the measurement of the preference for essential oil steering, Neroli (NE) showed the highest preference among the essential oils based on the hydrosol base ④ (Pancho: Nutmeg: Jilleoja = 2: 1: 1), and Next, it was confirmed that the fragrance steered with eucalyptus (EU) showed the highest preference.

Example 3: Investigation and Selection of Fragrance Preference According to Essential Oil Content

Fragrance consisting of neroli (NE) essential oil and eucalyptus (EU) essential oil based on ④ hydrosol base (Pancho: Nutmeg: Jilleoja = 2: 1: 1) adopted according to the results of Examples 1 and 2 above In addition to marjoram (MJ), lavender (LA), sweet orange (OR), and patchouli (PA) essential oils, the preference according to the oil content was investigated to determine whether the content was appropriate.

Specifically, a base was made by adding 40% vodka, which serves as a natural surfactant, in a 1: 1 ratio to the hydrosol ④ in Table 4 as a base. Then, the sensory test was conducted by dividing sample A containing 0.3 mL of neroli, eucalyptus, marjoram, lavender, sweet orange, and patchouli essential oils into sample B containing 0.6 mL, respectively, to determine which content was most preferred. did

As a result, as shown in Table 7 below, the fragrance preference according to the oil content was the highest in Sample A with 0.3 mL, No. 3 (LA) and No. 5 (MJ), but most of the participants in the sensory test showed that Sample A Many reviews said the scent was weak. On the other hand, in Sample B, No. 7 (EU) and No. 10 (NE) were the most preferred, and it was confirmed that the strength of the fragrance was the most appropriate.

In addition, as shown in Table 8 below, the likes and dislikes of sample A were not clear and the results were similar, but the likes and dislikes of sample B were also evident. It was confirmed that the most preferred in sample B were eucalyptus (No. 7, EU) and neroli (No. 10, NE).

Example 4: Investigation and selection of preference when essential oils are blended and steered

Based on the results of Table 8 of Example 3, it was confirmed that adding 0.6 mL of essential oil was the most preferred. Accordingly, the present inventors investigated the preference when blending and steering essential oils.

Specifically, eucalyptus (EU), neroli (NE), marjoram (MJ), sweet orange (OR), patchouli (PA), and lavender (LA) essential oils were adjusted to 0.6 mL and mixed in a 1:1 ratio. Eggplant essential oil was steered at 0.6 mL each. Thereafter, the preference of the fragrance through the sense of smell was measured at a distance of 30 cm from the 15 fragrances prepared in Table 9, that is, the steered sample. At this time, the measurement of preference for fragrance was performed in a closed space of 10 pyeong for a total of 20 people.

As a result, among the fragrances in Table 9 below, the fragrance consisting of prescription No. 3 (EU + NE), No. 5 (MJ + OR), No. 7 (LA + MJ), and No. 9 (NE + OR) was the most preferred. and the rest of the fragrances showed a slight reaction.

Fragrance No. 3 is a blend of Eucalyptus (EU) and Neroli (NE), Fragrance No. 5 is a blend of Marjoram (MJ) and Sweet Orange (OR), and Fragrance No. 7 is a blend of Lavender (LA) and Marjoram (MJ). and fragrance No. 9 was blended with neroli (NE) and sweet orange (OR).

On the other hand, the results obtained by converting the measured results to a scale of 100 are shown in Table 10 below.

Example 5: Preference investigation and selection of fragrances according to essential oil blending combinations

Preference for fragrances No. 3 (EU + NE), No. 5 (MJ + OR), No. 7 (LA + MJ), and No. 9 (NE + OR) among the herbal air fresheners for dogs prepared in Example 4 above. For the purpose of the investigation, a sensory test was conducted on 32 ordinary people. In order not to mix the scents, after preparing four rooms of about 10 pyeong, I went into each room and smelled the scent, and I was asked to choose the one I prefer the most. The results are shown in Table 11 below.

As can be seen from the above results, in Table 11, fragrance No. 3 blended with eucalyptus (EU) and neroli (NE) was the most preferred, followed by blending neroli (NE) and sweet orange (OR). It was confirmed that the preference for one fragrance was high.

Example 6: Orientation test of herbal air freshener for dogs

Based on the results of Examples 1 to 5, natural surfactants were mixed in the same ratio with a hydrosol base in which herbal fragrances for dogs, that is, Sancho, Nutmeg and Zillaja were mixed in a ratio of 2: 1: 1, and eucalyptus and neroli were mixed. The herbal fragrance blended with essential oils (prescription No. 3 in Table 11) was finally determined as the optimal herbal fragrance for dogs.

A sensory test was conducted for 15 days on a total of 53 dog owners living with their dogs in the house between the ages of 20 and 60 for the finalized herbal air freshener for dogs.

Specifically, after making 50 mL of the air freshener according to prescription No. 3 in Table 11, place it at a distance of 1 m next to the place where the dog stinks, smell (fragrance), freshness index, fragrance persistence, and whether it helps sleep was investigated. The degree of diffusion of the scent was investigated whether the scent could be recognized when the dog owner fell at 1 m intervals in a straight line from the standing position. The results were expressed in numbers and summarized in Tables 12 to 18 below.

Specifically, in terms of the degree of preference for smell (incense), the item of 'easily felt scent' received the greatest response (60%) (Table 12 and FIG. 1). In addition, the fragrance durability showed a positive response (74%) in the items of 'normally slight' to 'normally good' (Table 13 and FIG. 2).

The refreshment index showed a high vote rate (83%) in response to the items of 'slightly refreshing' to 'very refreshing' (Table 14 and FIG. 3). In addition, the degree of diffusion of the scent intensity showed a high vote rate (77%) for the items '1 m ~ 3 m' (Table 15 and FIG. 4).

On the other hand, as for the likes and dislikes of companion dogs, 'average' showed about half of the total vote (54%) (Table 16 and FIG. 5). In addition, in terms of whether or not it helps to remove the dog's odor, the items of 'normal' to 'very good' showed the most positive responses (79%) (Table 17 and FIG. 6).

In addition, as a result of examining the purchase intention, it was found that there is a possibility of commercialization as the items of 'somewhat' to 'must buy' showed positive responses (71%) (Table 18 and FIG. 7).

Example 7: Antibacterial test of air freshener for dogs

In order to confirm whether the antibacterial effect of the hydrosol containing the defense mechanism of natural herbal medicine actually works in the herbal fragrance for dogs, the antibacterial activity of the herbal fragrance for dogs was investigated.

Specifically, in order to increase the accuracy of antibacterial test results for prescription sample No. 3 in Table 11, samples A and B, which are identical samples, were divided into micro tubes, respectively, and minimum inhibitory concentrations (MICs) were measured. Super bacteria used in this antibacterial test were tested on standard strains. After taking the strain at -70 ℃ and smearing it on a solid medium, it was put into an incubator and cultured for 24 hours. After 24 hours, it was activated and used for 20 to 30 minutes. After adding 100 μl of liquid medium to a 96-well plate, samples and superbacteria standard strains were added. At this time, the sample (prescription sample No. 3 in Table 11) was set at a minimum of 10 μl and put in from 10 μl to 100 μl, and the strain was added in 10 μl increments according to the turbidity of 0.5 g. After culturing for 24 hours in an incubator set at 36.5° C., 10 μl of MTT reagent was added to confirm the antibacterial effect of the sample. There was no replacement of the medium during the experiment, and the experiment was repeated three times in total.

As a result, it was confirmed that herbal fragrance samples A and B for dogs according to sample No. 3 in Table 11 killed the standard super bacteria strain at 10 μl (FIG. 8). The effect was visible from the 2nd day, and the durability was about 7 days, showing an excellent antibacterial effect. On the other hand, when compared to the control groups Ampicillin (31.25 μl/ml) and Oxacillin (500 μl/ml), it was confirmed that Samples A and B showed superior antibacterial effects (FIG. 9).

Claims (11)

Herbal herbal base in the form of a hydrosol consisting of 1 part by weight of Japanese pepper hydrosol, 0.1 to 3 parts by weight of nutmeg hydrosol, and 0.1 to 3 parts by weight of japonica hydrosol, a natural surfactant of vodka or alcohol, and an essential oil consisting of eucalyptus oil and neroli oil. Herbal air freshener for dogs made of oil. delete According to claim 1, wherein the herbal medicine is extracted in the form of a hydrosol (hydrosol) using a water-cooled cooling circulation type extractor, herbal fragrance for dogs.
delete delete The oriental fragrance according to claim 1, wherein the alcohol content of the vodka or alcohol is 40%. The herbal fragrance for dogs according to claim 1, wherein the natural surfactant is mixed with the hydrosol type herbal medicine base in the same ratio. delete The herbal fragrance for dogs according to claim 1, wherein the essential oil is added in an amount of 0.5 to 1.5 parts by weight based on 100 parts by weight of a fragrance base in which a hydrosol type herbal medicine base and a natural surfactant are mixed in equal proportions. The herbal fragrance for dogs according to claim 1, wherein the herbal fragrance for dogs has antibacterial activity.
a) obtaining hydrosols of sancho, nutmeg and zillaja as herbal medicines;
b) preparing a hydrosol-type herbal medicine base by adding 0.1 to 3 parts by weight of nutmeg hydrosol and 0.1 to 3 parts by weight of Zillaja hydrosol based on 1 part by weight of Sancho hydrosol obtained above;
c) preparing a fragrance base by adding vodka or alcohol as a natural surfactant to the herbal medicine base in the same ratio; and
d) adding 0.5 to 1.5 parts by weight of essential oil blended with eucalyptus oil and neroli oil based on 100 parts by weight of the prepared fragrance base.

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