KR102462551B1 - Method of preparing a mealworm beetle feed for increasing fat content - Google Patents

Abstract

The present invention relates to a feed for Tenebrio molitor and a manufacturing method thereof, in which weight gain, fat content, and breeding efficiency can be increased by molding fermented wheat bran and processed food waste into a predetermined size.

Description

Method of preparing a feed for brown mealworm for increasing fat content {Method of preparing a mealworm beetle feed for increasing fat content}

The present invention relates to a feed for brown mealworm for increasing fat content and a method for manufacturing the same, and more particularly, to brown mealworm that has increased weight gain, fat content and breeding efficiency by molding fermented bran and processed food waste to a predetermined size. It relates to a feed and a method for manufacturing the same.

Recently, as competition for securing biological resources through the search and conservation of insect resources is getting fiercer, research on their value evaluation and use and development is being conducted very actively. As a part of this research, research on the development of food or pharmaceutical materials from insects is being conducted at home and abroad.

Mealworm (Tenebrionidae) is an insect belonging to the order Coleoptera, commonly found in warm and dry climates, and mostly feeds on dry and decaying plant or animal tissues. Caterpillars of this family (Tenebrionidae) have a length of about 25 mm and a cylindrical worm shape, and are often called mealworms, and the period is about 15 to 20 weeks, but the metamorphosis period is relatively short, so the growth is fast, and the reproduction rate is fast. It is also very high, and it is a clean insect that feeds on grain.

Most of the caterpillars of the family Mealworm are used as food for pet birds, fish, or hedgehogs, and among them, the larvae of the brown mealworm (Tenebrio molitor, mealworm, TM) are the most used. Brown mealworm, also called brown rice mealworm or Gosaeae, is distributed all over the world including Korea. Brown mealworm contains abundant fat, protein, various kinds of amino acids, unsaturated fatty acids and minerals.

Brown mealworm can be easily mass-produced and has a high nutritional value, so interest in the development of industrial uses such as feed and food is gradually increasing.

Meanwhile, biodiesel is being researched and commercialized as one of the energy sources that can replace fossil fuels. Biodiesel is produced by chemically transesterifying vegetable oil or animal fat with alcohol under various catalysts and reaction conditions. It is an eco-friendly alternative to petroleum fuel that contains oxygen in the molecule as well as a source. Insects, including brown mealworm, contain a large amount of fat, so they can be used as eco-friendly raw materials to produce such biodiesel.

However, in order to improve the economic efficiency and efficiency of biodiesel using insects, it is necessary to feed a feed that can increase the fat content of brown mealworm. In addition, since most of the existing insect feed is supplied in powder form (due to feed dust), the feeding operation is cumbersome and thus there is a problem in that efficiency is lowered.

The present invention is to provide a feed capable of increasing the weight gain and fat content of brown mealworm.

The present invention is to provide a feed for brown mealworm formulated in a predetermined size or more, not a powder.

The present invention relates to a feed for brown mealworm, which contains wheat bran, processed food waste, preservatives and solids, and is molded to a size of 3 mm or more in diameter.

The present invention provides a step of mixing bran and processed food waste in a weight ratio of 1: 0.5 to 2, Lactobacillus fermentum, Lactobacillus acidophilus, and pedio in the mixture of bran and food Inoculating one or more strains of Caucus acidilactici and fermenting for 4 to 28 days, 0.01 to 0.5% by weight of a preservative, 10 to 30% by weight of a solid agent and water are added to the mixture. It relates to a manufacturing method comprising the step of kneading, forming the kneaded mixture to a diameter of 3 mm or more and drying.

The feed for brown mealworm of the present invention increased the weight gain and fat content of brown mealworm by fermenting wheat bran and processed food waste into three strains. The feed for brown mealworm of the present invention increased the fat content by about 19% compared to the feed fed only wheat bran.

In addition, the feed for brown mealworm of the present invention did not reduce the weight gain or added preservatives and solid agents that could increase the weight gain, thereby improving feeding efficiency and convenience by formulating the feed into a predetermined size.

1 shows the manufacturing process of the present invention.
Figure 2 is a graph showing the increase in the amount of brown mealworm larvae reared with 7 types of feed mixed with 3 types of raw materials having excellent effect of increasing the weight gain.
3 is a graph showing the weight gain of brown mealworm fed feed fermented with EM.
4 is a graph showing the weight gain of brown mealworm fed feed fermented with yeast.
5 is a graph showing the weight gain of brown mealworm fed feed fermented with three strains.
6 is a graph showing the amount of weight gain of brown mealworm fed with a preservative-added feed.
7 is a graph showing the weight gain of brown mealworm fed with agar-added feed.
8 is a graph showing the weight gain of brown mealworm fed feed with carrageenan added.
9 is a graph showing the weight gain of brown mealworm fed with a feed containing potato starch.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in several different forms, and thus is not limited to the embodiments described herein.

When a part "includes" a certain component, it means that other components may be further included without excluding other components unless otherwise stated.

The feed for brown mealworm of the present invention includes bran, processed food waste, preservatives and solids.

The bran is also called wheat bran, and is generally used as a feed as a residue remaining after milling and sieving wheat. The remainder of the separation of wheat flour and germ from the milled wheat to form a powder. Most of the components are the outer skin of the seed, and it has a small amount of endosperm and embryo. The amount and composition of bran that can be obtained from wheat varies depending on the proportion of the milled product, but generally contains 15% protein, 4% fat, 25% carbohydrate, 10% fiber, etc.

The processed food waste is a dry feed or liquid feed that is produced through a processing process (separation, crushing, drying, etc.) of the collected food waste and feedable to livestock. For example, after collecting food waste discharged from homes, restaurants, and group cafeterias, the collected food waste is separated from foreign substances, then cut into small pieces with a grinder, and the crushed food waste is squeezed to remove moisture and dried by heating. Thus, it is possible to produce processed food waste for feed.

In general, as feed for brown mealworm, wheat bran and agricultural by-products (rice bran, cornstalk, soybean meal, sesame meal, perilla meal, bean curd meal), coffee meal, and fecal soil are used. As a result of measuring the weight gain after feeding these feed materials to brown mealworm (see Example), when bran, food waste and fecal soil were fed, it showed a higher weight gain increase rate compared to the rest of the feed (rice bran, corn, sesame meal, etc.) .

In addition, as a result of analyzing the components of brown mealworm larvae fed with these feed materials, the fat content was highest in the order of food waste, rice bran, bran, corn, and fecal soil. However, the weight gain of rice bran was only about 47% and 65% of that of bran.

In the present invention, three feed ingredients, which can increase the weight gain and fat content of brown mealworms at the same time, are combined and fed to brown mealworms alone (WB), food waste (FW), and fecal soil (TF). was measured (refer to FIG. 2 and Table 6). Referring to FIG. 2 and Table 6, it can be seen that the highest weight gain and the highest fat content is a 1:1 combination of bran (WB) and food waste (FW).

As described above, the feed of the present invention can increase the weight gain and fat content of brown mealworm by combining bran and processed food waste.

In the feed for brown mealworm of the present invention, bran and processed food waste may be mixed in a weight ratio of 1: 0.1 to 10, preferably 1: 0.5 to 2, more preferably 1:1.

The mixture of bran and food is fermented for 4 to 28 days with any one or more strains of Lactobacillus fermentum, Lactobacillus acidophilus and Pediococcus acidilactici can be

In addition, the feed of the present invention may be inoculated with each strain in the bran and processed food waste, fermented, and then mixed with them.

Lactobacillus fermentum (Lactobacillus fermentum), Lactobacillus acidophilus (Lactobacillus acidophilus) and Pediococcus acidilactici strains have excellent protein and lipid fermentation efficacy.

In addition, when the bran and food were fermented using Lactobacillus fermentum, Lactobacillus acidophilus and Pediococcus acidilactici strains, Compared to the use of useful microorganisms (EM) and yeast, the weight gain of brown mealworm was more increased.

The preservative should not reduce the weight gain of brown mealworm when fed as feed, and it is preferable to use one that increases the weight gain.

For example, the preservative may be sorbic acid, sodium benzoate, calcium or sodium propionate, formaldehyde, methylparaben, or grapefruit extract. .

The preservative may be included in the feed 0.01% to 0.5% by weight, preferably 0.05% to 0.2% by weight.

The solid agent may make the feed composition in powder or liquid form into a solid or gel form. That is, the solid agent may be formulated into a powder feed or liquid feed in a predetermined size.

The feed for brown mealworm of the present invention may be molded to a diameter of 3 mm or more, preferably 3 mm to 100 mm.

The solid agent includes agar, carrageenan, starch, alginic acid, gelatin, guar gum, glutinous, soya lectin, carboxymethyl cellulose, and the like.

The solid agent may be included in 10% to 30% by weight of the feed, preferably 15% to 30% by weight, more preferably 15 to 25% by weight.

In another aspect, the present invention relates to a method for producing a feed for brown mealworm. Referring to FIG. 1 , the manufacturing method of the present invention includes a mixing step, a fermentation step, a kneading step and a forming step.

The mixing step is a step of mixing the bran and processed food waste in a weight ratio of 1: 0.5-2. For a mixture of bran and processed food waste, see Brown Mealworm Feed.

In the fermentation step, any one or more strains of Lactobacillus fermentum, Lactobacillus acidophilus, and Pediococcus acidilactici in a mixture of the bran and processed food waste It is a step of inoculating and fermenting for 4 to 28 days, preferably 4 to 10 days.

The kneading step is a step of kneading the fermented product by adding 0.01 to 0.5% by weight of a preservative, 10 to 30% by weight of a solid agent and water. For example, in the kneading step, water is mixed with a mixture of fermented wheat bran, food waste, preservatives and solids in a ratio of 1:1 to 2 (w/v), 1:1 (w/v) and kneaded for about 60 minutes. do.

The forming step is a step of forming the dough to a diameter of 3 mm or more and drying. The size of the formulated feed may be 3 mm or more, preferably 3-100 mm. For example, drying may remove moisture by applying heat at 60° C. for 50 minutes after molding is completed.

For details of the mixing step, fermentation step, kneading step and forming step, you can refer to the feed for brown mealworm described above.

Through the following examples, the present invention will be described in more detail, but it is obvious that the present invention is not limited by the following examples.

(A) Analysis of the composition of 10 kinds of raw materials to increase economic efficiency and breeding efficiency

To increase economic efficiency and breeding efficiency, wheat bran and agricultural by-products (rice bran, cornstalk, soybean meal, sesame meal, perilla meal, bean curd meal) used in conventional brown mealworm breeding, coffee meal, fecal soil, and food waste dry feed (Hindol Co., Ltd.) , Cheonan) were selected (Table 1) and the composition was analyzed (Table 2). As a result of analyzing the composition of 10 feed materials, rice bran (21.81%) contained the highest amount of fat, followed by sesame meal and perilla meal, which are by-products after milking, and food waste material also showed a fat content of 8.10% ( Table 2).

treatment area feed treatment area feed One rice bran 6 perilla gourd 2 bran 7 biji squash 3 corn 8 coffee gourd 4 Soybean Meal 9 fecal soil 5 sesame gourd 10 Food waste

(g/100g) calorie carbohydrate protein Fat ash rice bran 471.44±0.49 ^a 52.99±0.05 ^c 15.69±0.20 ^ef 21.81±0.13 ^a 9.51±0.03 ^b bran 399.80±0.89 ^CD 76.17±0.33 ^a 14.99±0.02 ^f 3.95±0.24 ^d 4.89±0.07 ^f corn 378.05±12.35 ^de 78.56±4.33 ^a 8.26±1.38 ^g 3.41±0.11 ^d 6.53±0.19 ^d Soybean Meal 383.79±1.75 ^cde 21.19±1.89 ^e 68.31±1.97 ^a 2.86±0.14 ^e 7.64±0.21 ^c sesame gourd 403.71±0.17 ^c 26.47±1.94 ^e 51.84±2.53 ^b 10.02±0.26 ^c 11.66±0.33 ^a perilla gourd 428.43±1.10 ^b 39.27±1.34 ^d 44.41±1.06 ^c 10.41±0.23 ^c 5.90±0.05 ^e biji squash 404.53±0.35 ^c 61.03±1.68 ^b 31.21±1.82 ^d 3.96±0.09 ^d 3.81±0.05 ^g coffee gourd 478.73±0.51 ^a 65.06±0.56 ^b 16.60±0.63 ^ef 16.94±0.10 ^b 1.40±0.02 ^h fecal soil 362.23±15.77 ^e 68.08±3.51 ^b 20.09±0.22 ^e 1.07±0.06 ^f 6.60±0.37 ^d Food waste 384.74±0.91 ^cde 61.43±1.28 ^b 16.53±1.91 ^ef 8.10±0.42 ^c 7.98±0.08 ^c

(B) Analysis of larval growth and composition after feeding 10 kinds of raw materials to increase economic efficiency and breeding efficiency

Table 3 compares the weight gain of brown mealworms bred with 10 kinds of raw materials. As a result of checking the weight gain (Table 3), bran 108.83%, fecal soil 93.25%, food waste 86.72%, corn 70.21%, perilla meal 69.32%, bean curd meal 55.13%, rice bran 51.30%, sesame meal 37.38%, coffee meal 36.69%, Soybean meal was found to be 22.56%, so the feed with the best effect of increasing weight was wheat bran, followed by fecal soil and food waste.

5 days later after 10 days 15 days later 20 days later rice bran 10.61 ± 1.27 23.28 ± 10.29 27.24 ± 16.33 51.30 ± 12.09 ^d bran 40.53 ± 8.18 54.88 ± 7.91 93.66 ± 28.42 108.33 ± 14.29 ^a corn 16.48 ± 6.33 49.12 ± 3.69 55.57 ± 5.93 70.41 ± 3.85 ^b Soybean Meal 12.69 ± 8.27 19.97 ± 10.40 20.57 ± 9.92 22.56 ± 7.57 ^e sesame gourd 8.16 ± 6.54 15.86 ± 12.86 46.71 ± 19.70 37.24 ± 18.95 ^de perilla gourd 26.13 ± 13.16 31.41 ± 15.01 62.94 ± 14.63 69.32 ± 21.33 ^bc biji squash 8.67 ± 7.88 14.53 ± 5.69 39.94 ± 13.82 55.13 ± 10.67 ^CD coffee gourd 14.56 ± 12.34 24.11 ± 11.71 40.80 ± 9.73 36.69 ± 16.21 ^de fecal soil 36.32 ± 10.65 44.85 ± 11.98 59.91 ± 14.16 93.25 ± 10.29 ^a Food waste 28.57 ± 8.89 39.11 ± 11.17 69.45 ± 10.92 86.72 ± 18.79 ^ab

Table 4 shows the compositional analysis results of brown mealworm larvae reared with 10 kinds of raw materials. It was confirmed that the fat content in the body of brown mealworm larvae was the highest when food waste was fed as it showed a different pattern from the result of the previous weight gain increase effect (Table 3).

(g/100g) treatment area Calories (Kcal) carbohydrate protein Fat ash moisture rice bran 564.00±2.64 ^a 14.41±1.02 ^bcd 44.53±0.79 ^d 36.81±0.59 ^a 3.09±0.02 ^CD 1.94±0.05 ^g bran 533.33±3.51 ^b 11.86±0.95 ^d 51.70±0.21 ^c 30.98±0.71 ^b 3.99±0.00 ^abcd 1.46±0.03 ^h corn 522.00±2.65 ^c 11.98±0.62 ^d 50.75±0.11 ^c 30.14±0.52 ^b 4.31±0.00 ^abcd 2.82±0.03 ^f Soybean Meal 419.67±2.52 ^g 16.45±1.23 ^bc 62.05±0.74 ^a 11.74±0.50 ^e 5.18±0.01 ^abc 4.57±0.02 ^c sesame gourd 433.67±1.53 ^f 12.05±0.67 ^d 60.70±0.96 ^a 15.86±0.30 ^d 6.07±0.05 ^a 5.31±0.02 ^a perilla gourd 451.67±1.53 ^e 16.90±1.16 ^bc 55.90±1.69 ^b 17.87±0.41 ^d 5.76±0.16 ^ab 3.57±0.04 ^d biji squash 411.00±1.73 ^gh 24.24±0.94 ^a 56.05±0.61 ^b 9.98±0.34 ^ef 4.55±0.03 ^d 5.18±0.04 ^a coffee gourd 407.67±3.06 ^h 18.05±1.05 ^b 63.00±0.57 ^a 9.28±0.58 ^f 5.14±0.01 ^abc 4.53±0.04 ^c fecal soil 509.33±7.37 ^d 13.64±1.19 ^CD 51.37±0.43 ^c 27.70±1.56 ^c 4.13±0.02 ^abcd 3.15±0.01 ^e Food waste 515.78±2.99 ^CD 16.31±1.59 ^bc 39.40±0.92 ^e 38.08±0.91 ^a 3.51±0.68 ^bcd 5.04±0.05 ^b

(C) Blend of 3 types of raw materials with excellent weight gain among 10 types of raw materials

Out of the 10 raw materials (Table 2), the effect of increasing the weight gain of brown mealworm is excellent (Table 3), wheat bran (WB) (108.33%), Tenebrio molitor fecal soil (TFS) (93.25%), food waste ( Food waste, FW) (86.72%) was mixed to find the optimal combination to maximize weight gain and fat content, 7 types of feed were prepared and the composition was analyzed and shown in Table 5.

(g/100g) treatment area Calories (Kcal) carbohydrate protein Fat ash WB 399.80 76.17 14.99 3.95 4.89 TFS 351.12 69.51 11.15 12.27 7.37 FW 384.74 61.43 16.53 8.10 7.98 WB/TFS (50/50) 375.46 72.84 13.07 8.11 6.13 WB.FW (50/50) 392.27 68.80 15.76 6.03 6.44 TFS/FW (50/50) 367.93 65.47 13.84 10.19 7.68 WB/TFS/FW (50/25/25) 383.87 70.82 14.41 7.07 6.28

(D) Analysis of weight gain and composition after feeding 7 types of feed prepared by mixing 3 types of raw materials with excellent weight gain among 10 types of raw materials Bran (WB), fecal soil (TF) with excellent effect of increasing brown mealworm weight after breeding with 10 types of raw materials , 3 types of food waste (FW) were selected, and a breeding experiment was conducted for 20 days with 7 types of feed (Table 4) prepared by mixing them in various ratios, and the amount of weight gain at 5-day intervals after feeding (days post feeding, DPF) was measured. Weight gain was WB 370.74%, WB/FW 368.57%, FW 339.77%, TF 289.37%, WB/TF 267.84%, WB/TF/WF 21.92%, TF/FW 173.22% (FIG. 2), and WB and The treatment with similar weight gain was WB/FW, and since the price of FW (50 won/kg) raw material is cheaper than WB (200 won/kg), it is considered more economical to use WB/FW.

Table 6 shows the component analysis of brown mealworm larvae bred with 7 types of feed prepared by mixing 3 types of raw materials. Referring to Table 6, it was confirmed that the fat content of brown mealworm was high in the feed treatment group containing food waste (FW). That is, referring to FIG. 2 and Table 6, it was determined that the bran/food waste mixture (WB/FW), which was excellent in both weight gain and fat content increasing effect, was economically and efficiently optimal for breeding brown mealworm.

(g/100g) treatment area protein Fat moisture ash Calories (Kcal) WB 51.51 ± 0.05 ^b 25.17 ± 0.08 ^c 15.13 ± 0.05 ^c 4.36 ± 0.02 ^b 493 ± 1.25 ^a TFS 56.40 ± 0.06 ^a 18.97 ± 0.24 ^d 16.06 ± 0.08 ^b 5.11 ± 0.21 ^a 255 ± 1.65 ^d FW 37.75 ± 0.02 ^d 36.28 ± 0.34 ^a 18.57 ± 0.28 ^a 3.54 ± 0.01 ^c 415 ± 5.23 ^b WB/TFS (50/50) 53.91 ± 1.05 ^a 23.73 ± 0.48 14.43 ± 0.61 ^d 4.58 ± 0.02 ^b 290 ± 1.29 ^d WB.FW (50/50) 46.61 ± 0.02 ^c 29.12 ± 0.25 ^b 16.48 ± 0.27 ^b 4.86 ± 0.01 ^b 347 ± 0.28 ^c TFS/FW (50/50) 47.68 ± 0.57 ^c 29.93 ± 0.68 ^b 15.01 ± 0.07 ^c 4.45 ± 0.03 ^b 347 ± 0.98 ^c WB/TFS/FW (50/25/25) 50.67 ± 0.24 ^b 25.55 ± 0.29 ^c 15.77 ± 1.24 ^c 4.61 ± 0.01 ^b 311 ± 1.27 ^c

(E) Establishment of fermentation conditions to increase feed efficiency Six strains (Park et al., 2005; Kim et al., 2005; Kim et al. ., 2018; Jeong et al., 2017) were selected to evaluate their fermentability and are shown in Table 7 below. Referring to Table 7, the fermentation ability of carbohydrates (amylase, maltase, sucrase) was found to be excellent in L. fermentum and L. acidophilus, and the fermentation efficiency of protein (protease) and lipid (lipase) was found in L. fermentum, L. fermentum, L. acidophilus and P. acidiloctici were excellent, and L. fermentum, L. acidophilus, and P. acidiloctici were selected as strains for fermentation of feed for brown mealworm.

Amylase Maltase Sucrase Proteases Lipase L. plantarum 3.47 ± 0.24 ^b 35.28 ± 3.84 ^b 44.09 ± 3.35 ^a 107.70 ± 0.48 ^a 0.086 ± 0.001 ^b L. fermentum 4.01 ± 0.50 ^a 85.13 ± 6.89 ^a 24.94 ± 1.45 ^c 112.35 ± 0.82 ^a 0.094 ± 0.002 ^a L. acidophilus 3.56 ± 0.12 ^b 15.58 ± 3.56 ^d 73.96 ± 0.12 ^a 116.87 ± 0.98 ^a 0.093 ± 0.001 ^a P. acidiloctici 2.77 ± 0.29 ^c 28.98 ± 7.34 ^c 43.24 ± 3.52 ^b 112.59 ± 0.74 ^a 0.093 ± 0.001 ^a B. subtilis 3.11 ± 0.09 ^b 25.27 ± 1.01 ^c 46.31 ± 0.24 ^b 100 ± 0.00 ^b 0.090 ± 0.001 ^a S. cerevisiae 3.34 ± 0.12 ^b 42.68 ± 2.64 ^b 42.70 ± 0.80 ^b 106.85 ± 0.57 ^a 0.096 ± 0.002 ^a

50 selected 3 types of fermentation strains (3 microorganisms, 3M) and effective microorganisms (EM) and yeast (yeast, Y) 1×106 CFU/mL used in the existing feed fermentation were added to the bran/food waste mixed feed. It was added at a volume of mL/kg and fermented for 5, 7, 14, 21, and 28 days (days after fermentation, DAF). 3 is a graph showing the weight gain of brown mealworm fed feed fermented with EM, FIG. 4 is a graph showing the weight gain of mealworm fed feed fermented with yeast, and FIG. 5 is a feed fermented with 3 strains. It is a graph showing the weight gain of fed brown mealworm. Referring to FIGS. 3 to 5 , for each fermented strain, the weight gain of the mixed feed fermented with 3M was generally high, and by fermentation period, it was found that the effect of increasing the weight gain was greatest at 5 DAF. The shorter the fermentation period, the higher the economic efficiency, and the greater the effect of increasing the weight gain, the more efficient the 3M/5 DAF mixed feed was selected as the feed for brown mealworm for bioenergy production.

(F) Establishment of conditions for adding preservatives to target raw materials

After mixing 0.05%, 0.10%, and 0.15% of sorbic acid (S), which is commonly used in insect feed, and grapefruit seed extract (GSE), which is known as a naturally derived preservative, respectively, it is fed to brown mealworms to prevent larvae. A comparative analysis of the weight gain is shown in FIG. 6 . Referring to FIG. 6 , the weight gain was the highest when 0.1% of GSE was added, but there was no significant difference in all treatment groups. Since the prices of sorbic acid and grapefruit seed extract are 7,100 won and 11,500 won based on 1 kg, respectively, it was found that sorbic acid is more economical. Since there was no significant difference in the effect of increasing the weight gain in the treatment group treated with the preservative, 0.05% of sorbic acid, which is inexpensive and contains the least amount of preservative, is highly economical and was selected as the preservative.

(G) Establishment of solidification conditions for brown mealworm artificial feed

To establish the solidification conditions of the fermented wheat bran/food waste (FWBFW) of the brown mealworm, agar (agar, A), carrageenan (carrageenan, C), and potato starch (starch, S) were added to 25 of the feed amount. %, distilled water was added at 1:1 (w/v) of the feed amount, kneaded with a large-capacity kneader for 1 hour, and then extracted to a size of 6 mm using a mincer. The extracted feed was dried at 60℃ for 50, 100, and 150 minutes, respectively, and then cut into small pieces and fed to brown mealworm. As a result of confirming the weight gain of brown mealworm fed feed with three solidification materials added (FIG. 7 is agar, FIG. 8 is carrageenan, FIG. 9 is brown mealworm weight gain fed feed containing potato starch) according to solidification material There was no significant difference in weight gain. However, it was confirmed that the shorter the drying time, the significantly increased the weight gain. This is thought to be because moisture remains in the feed when it is dried for a short time. Considering the price of agar (6,380 won/100 g), carrageenan (10,900 won/100 g), and potato starch (7,900 won/100 g) used in this experiment, agar was the most economical, and there was a significant difference in the amount of weight gain depending on the solidification material. Since there was no solidification material, agar was selected.

(H) Analysis of the composition of brown mealworm raised with solidified feed and bran

Bran (WB) and food waste (FB) raw materials were mixed in a 1:1 ratio. Three strains (L. fermentum, L. acidophilus, P. acidiloctici) 1×10 ⁶ CFU/mL were inoculated into WBFW at a dose of 50 mL/kg and fermented for 5 days (FWBFW). After the fermentation is completed, 0.05% of sorbic acid is added as a preservative to FWBFW (FWBFW-S), and then 25% of agar is added (FWBFW-SA). After that, the mixture and distilled water are mixed at a ratio of 1:1 (w/v) and kneaded in a kneader for 60 minutes. After kneading, the FWBFW-SA was molded to a diameter of 6 mm and dried at 60°C for 50 minutes (FWBFW-SA50) and refrigerated at 4°C.

By comparing the fat content of brown mealworms fed with fermented wheat bran/food waste (FWBFW)/S/A/50 minutes (FWBFWSA50) feed prepared above and brown mealworms reared with wheat bran (WB), It is shown in Table 8 below.

Referring to FIG. 8 , it was confirmed that the fat content of brown mealworm fed with FWBFWSA50 feed was significantly increased. Therefore, when the feed of the present invention is fed to brown mealworm, there is an effect of increasing the weight gain and fat content, and when used for mass breeding of brown mealworm for bioenergy production, it is shown to have an effect of increasing economic efficiency and efficiency.

crude protein george carbohydrate views calorie WB 54.47 ± 0.22 29.44 ± 0.73 12.04 ± .096 4.05 ± 0.00 527.67 ± 6.80 FWBFWSA50 52.70 ± 0.20 34.98 ± 0.7 ^* 10.86 ± 0.95 3.99 ± 0.00 545.49 ± 6.96

Embodiments of the present invention disclosed in the present specification and drawings are merely provided for specific examples in order to easily explain the technical contents of the present invention and help the understanding of the present invention, and are not intended to limit the scope of the present invention. In addition to the embodiments disclosed herein, it is apparent to those of ordinary skill in the art that other modifications based on the technical spirit of the present invention can be implemented.

Claims (2)

delete mixing the bran and processed food waste in a weight ratio of 1:1;
After inoculating the mixture of the bran and the processed food waste with three strains of Lactobacillus fermentum, Lactobacillus acidophilus and Pediococcus acidilactici Fermentation for 4 to 28 days;
kneading the fermented product by adding 0.05 to 0.15% by weight of a preservative, 10 to 30% by weight of a solid agent and water;
As a method for producing a feed for brown mealworm, comprising the step of molding the dough into a size of 3 mm to 100 mm in diameter and drying it,
The processed food waste is dry feed or wet feed in which the collected food waste is processed,
The preservative is sorbic acid or grapefruit seed extract (GSE)),
The feed for brown mealworm is a method for producing a feed for brown mealworm, characterized in that it can increase the weight gain and fat content of the brown mealworm.

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