CN117356655A - Silage mulberry branch and leaf feed for beef cattle and preparation method thereof - Google Patents
Silage mulberry branch and leaf feed for beef cattle and preparation method thereof Download PDFInfo
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- CN117356655A CN117356655A CN202310967052.5A CN202310967052A CN117356655A CN 117356655 A CN117356655 A CN 117356655A CN 202310967052 A CN202310967052 A CN 202310967052A CN 117356655 A CN117356655 A CN 117356655A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/22—Compounds of alkali metals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses silage mulberry branch and leaf feed for beef cattle and a preparation method thereof, wherein the feed comprises 30-35% of silage mulberry branch and leaf, 30-35% of dried straw, 24-25% of corn, 2-3% of wheat bran, 5-6% of bean pulp, 2-3% of cotton seed cake, 3-4% of vegetable pulp, 0.3% of salt, 0.9% of stone powder, 1% of premix and 100% of total. The feed prepared by the method has reasonable components, rich nutrition and good palatability, and can obviously reduce the use amount of silage corn under the condition that the production performance of beef cattle is not reduced, thereby reducing the use amount of feed grains and being beneficial to relieving the problem of grain contending between people and livestock; the feed prepared by the invention contains rich protein and amino acid, can balance the daily ration structure, and improves the digestibility of crude protein, crude ash, neutral washing fiber and acidic washing fiber of beef cattle. The preparation method of the invention has strong operability and is suitable for industrial production.
Description
Technical Field
The invention belongs to the field of animal feeds, and particularly relates to silage mulberry branch and leaf feed for beef cattle and a preparation method thereof.
Background
With the gradual improvement of the living standard and the gradual improvement of the dietary structure of people, the beef is favored by people because of being rich in high-quality protein, B vitamins and various microelements, and the gradual increase of the consumption of the beef drives the development of beef breeding industry. In the beef cattle cultivation process, the storage of feed, which is the key for ensuring the growth of beef cattle, cannot be ignored. However, in recent years, the supply of grain raw materials such as domestic corn, bean pulp and the like is greatly challenged by the influence of global climate change and international situation, the cultivation cost is continuously increased, and the development of the livestock industry is severely restricted. Strengthen the development of unconventional feed, and is an effective way for relieving the supply of conventional feed raw materials and reducing the cost of the feed.
The feed mulberry belongs to Moraceae mulberry genus, and is a deciduous arbor with strong stress resistance and wide suitable cultivation range. The leaves are also known as protein mulberry because they are rich in proteins. The mulberry leaves as animal feed have the characteristics of containing a plurality of natural active substances with special effects, such as sterols, isoquercitrin, quercetin Pi Gusu, gamma-amino acids and the like, having the effects of immunity and health care, remarkably reducing the dosage of antibiotics and improving the quality of beef cattle. However, mulberry branches are hard in wood, difficult to digest and poor in palatability, and the digestibility of directly fed beef cattle is low (1, chen Renjie, guo Qun. A method for preparing biological feed for beef cattle by using mulberry stalks, mulberry leaves and urea [ P ] Guangxi Zhuang nationality autonomous region: CN103652434B,2016-01-20.; [2] Cai Ming. Safety of mulberry leaves as animal feed is evaluated and studied [ D ] university of Lanzhou, 2019.). The silage special for beef cattle is developed by utilizing the feed mulberry, so that the feeding value of the silage can be effectively improved, the feed grain consumption is further reduced, and the sustainable development of beef cattle cultivation is promoted.
Disclosure of Invention
Based on the current situation of grain competition between people and livestock in China, the current cost-reducing and synergy requirements of beef cattle breeding industry and the development and development basis of feed mulberry development and utilization, the invention aims to provide silage mulberry branch and leaf feed for beef cattle and a preparation method thereof, so that the consumption of feed grain is reduced, and the problem of grain competition between people and livestock is facilitated to be relieved. In addition, the feed of the invention contains abundant protein and amino acid, can balance the ration structure and improve the digestibility of crude protein, crude ash, neutral washing fiber and acid washing fiber of beef cattle.
In order to achieve the above purpose, the technical scheme provided by the invention is as follows: a method for preparing silage mulberry branches and leaves, which comprises the following steps:
(a) Harvesting when the feed mulberry grows to 80-100 cm high or 30-45 days, and cutting mulberry branches and leaves into 2-3 cm after harvesting;
(b) Preparing raw materials of silage bacterial enzyme: the silage bacterial enzyme agent comprises the following raw materials in parts by mass: 2-4 parts of lactobacillus plantarum, 2-4 parts of lactobacillus acidophilus, 2-3 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5-1 part of xylanase and 0.5-1 part of laccase;
(c) Reviving the strain: pouring the silage bacterial enzyme into 1% syrup, fully dissolving, and standing for 1-2 h at normal temperature, wherein the proportion of the silage bacterial enzyme to the 1% syrup is as follows: 1:2000 (w/v, g/mL).
(d) Preparing silage bacterial liquid: pouring the revived strain into 0.7-1.0% saline solution, stirring uniformly, and preparing the prepared bacterial solution which cannot be used overnight; wherein, the ratio of the revived strain to the saline solution is as follows: 2:1 (v/v, mL/mL).
(e) Spraying silage bacterial liquid into mulberry branches and leaves, uniformly mixing, controlling the water content of the mixture to be 60% -70%, and obtaining
Silage mulberry branch and leaf mixture;
(f) Dividing, bundling and profiling silage mulberry branch and leaf mixture, and sleeving a double-layer bag for sealing;
(g) Fermenting at 35-37deg.C for 30-40 days, and stopping anaerobic fermentation when pH is 4.0-4.5 to obtain silage mulberry branch and leaf.
In the preparation method of the silage mulberry branches and leaves, preferably, the silage bacterial agent comprises the following raw materials in parts by mass: 3 parts of lactobacillus plantarum, 3 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5 part of xylanase and 0.5 part of laccase.
In the preparation method of the silage mulberry branches and leaves, preferably, the silage bacterial agent comprises the following raw materials in parts by mass: 2 parts of lactobacillus plantarum, 4 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5 part of xylanase and 1 part of laccase.
In the preparation method of the silage mulberry branches and leaves, preferably, the silage bacterial agent comprises the following raw materials in parts by mass: 4 parts of lactobacillus plantarum, 2 parts of lactobacillus acidophilus, 3 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 1 part of xylanase and 0.5 part of laccase.
In the preparation method of the silage mulberry branches and leaves, preferably, the total viable count content of lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis in the silage bacterial agent is more than 2 multiplied by 10 10 CFU/g。
In the preparation method of the silage mulberry branches and leaves, preferably, in the step (f), the silage mulberry branch and leaf mixture is divided and bundled into 65 kg/bag, and the compression specification is 70cm by 40cm by 30cm.
The invention also provides silage mulberry branches and leaves prepared by the silage mulberry branches and leaves preparation method.
Meanwhile, the invention also provides silage mulberry branch and leaf feed for beef cattle, which comprises the following components in percentage by mass: the silage mulberry branch and leaf 30-35%, dried straw 30-35%, corn 24-25%, wheat bran 2-3%, soybean meal 5-6%, cotton seed cake 2-3%, rapeseed meal 3-4%, salt 0.3%, stone powder 0.9%, premix 1% and total 100%.
The silage mulberry branch and leaf feed for beef cattle preferably comprises the following components in percentage by mass: the silage mulberry branch and leaf 30%, dried straw 30%, corn 24.5%, wheat bran 2%, soybean meal 5.8%, cotton seed cake 2%, rapeseed meal 3.5%, salt 0.3%, stone powder 0.9%, beef cattle premix 1% and total 100% of claim 7.
The invention also provides a preparation method of the silage mulberry branch and leaf feed for beef cattle, which is characterized by comprising the following steps:
(1) Crushing corn, wheat bran, bean pulp, cotton seed cake and rapeseed meal, sieving, mixing, stirring uniformly, and adding salt, stone powder and beef cattle premix;
(2) And (3) mixing the material obtained in the step (1) with the silage mulberry branches and leaves and the dry straws, uniformly stirring to obtain mixed feed, and tamping to obtain the silage mulberry feed for beef cattle.
The invention has the following beneficial effects:
compared with the prior art, the feed prepared by the method has reasonable components, rich nutrition and good palatability, and can ensure that the use amount of silage corns is obviously reduced under the condition that the production performance of beef cattle is not reduced, thereby reducing the use amount of feed grains and being beneficial to relieving the problem of grain contending of people and livestock; the feed prepared by the invention contains rich protein and amino acid, can balance the daily ration structure, and improves the digestibility of crude protein, crude ash, neutral washing fiber and acidic washing fiber of beef cattle. The preparation method of the invention has strong operability and is suitable for industrial production.
Detailed Description
In the present invention, all raw material components are commercially available products well known to those skilled in the art unless specified otherwise. The present invention will now be described in detail by way of specific examples, which should be understood by those skilled in the art as being preferred, but not limiting.
Preparation example 1
The silage mulberry branches and leaves of the embodiment are prepared by the following method:
(a) The mulberry leaves are harvested when the feed mulberry grows to 80-100 cm (or 30-45 days), and the mulberry leaves are kneaded and cut to be about 2.5cm after being harvested.
(b) Preparing raw materials of silage bacterial enzyme: the silage bacterial enzyme agent comprises the following raw materials in parts by weight: 3 parts of lactobacillus plantarum, 3 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5 part of xylanase and 0.5 part of laccase. Wherein the total viable count content of Lactobacillus plantarum, lactobacillus acidophilus and Bacillus subtilis is greater than 2×10 10 CFU/g。
(c) Reviving the strain: pouring the silage bacterial enzyme into 1% syrup, fully dissolving, and standing for 2 hours at normal temperature, wherein the proportion of the silage bacterial enzyme to the 1% syrup is as follows: 1:2000 (w/v, g/mL).
(d) Preparing bacterial liquid: pouring the revived strain into 0.9% saline solution, stirring, and making the prepared strain solution unable to stay overnight; wherein, the ratio of the revived strain to the saline solution is as follows: 2:1 (v/v, mL/mL).
(e) Spraying silage bacterial liquid into mulberry branches and leaves, and uniformly mixing to control the moisture content of the mixture to be 60% -70% (about 65% in the embodiment) so as to obtain silage mulberry branch and leaf mixture;
(f) Dividing and bundling (65 kg/bag) silage mulberry branch and leaf mixture, compacting (70 cm in specification: 40cm in specification: 30 cm), and sealing with double-layer bag;
(g) Fermenting for 30 days at 37 ℃ and stopping anaerobic fermentation when the pH value is 4.0-4.5, so as to obtain silage mulberry branches and leaves.
Preparation example 2
The silage mulberry branches and leaves of the embodiment are prepared by the following method:
(a) Harvesting when the feed mulberry grows to 80-100 cm high or 30-45 days, and cutting mulberry branches and leaves into 2-3 cm after harvesting;
(b) The silage bacterial enzyme agent comprises the following components in parts by weight: 2 parts of lactobacillus plantarum, 4 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase and hemicellulase1 part of amylase, 1 part of xylanase, 0.5 part of laccase and 1 part of laccase. The total viable count content of Lactobacillus plantarum, lactobacillus acidophilus and Bacillus subtilis is greater than 2×10 10 CFU/g。
(c) Reviving the strain: pouring the silage bacterial enzyme into 1% syrup, fully dissolving, and standing for 2 hours at normal temperature, wherein the proportion of the silage bacterial enzyme to the 1% syrup is as follows: 1:2000 (w/v, g/mL).
(d) Preparing bacterial liquid: pouring the revived strain into 1000ml of 1.0% saline solution, stirring, and making the prepared strain solution incapable of overnight; wherein, the ratio of the revived strain to the saline solution is as follows: 2:1 (v/v, mL/mL).
(e) Spraying silage bacterial liquid into mulberry branches and leaves, and uniformly mixing to control the moisture content of the mixture to 70% so as to obtain silage mulberry branch and leaf mixture;
(f) Dividing and bundling (65 kg/bag) silage mulberry branch and leaf mixture, compacting (70 cm in specification: 40cm in specification: 30 cm), and sealing with double-layer bag;
(g) Fermenting for 30 days at 37 ℃ and stopping anaerobic fermentation when the pH value is 4.0-4.5, so as to obtain silage mulberry branches and leaves.
Preparation example 3
The silage mulberry branches and leaves of the embodiment are prepared by the following method:
(a) Harvesting when the feed mulberry grows to 80-100 cm high or 30-45 days, and cutting mulberry branches and leaves into 2-3 cm after harvesting;
(b) The silage bacterial enzyme agent comprises the following components in parts by weight: 4 parts of lactobacillus plantarum, 2 parts of lactobacillus acidophilus, 3 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 1 part of xylanase and 0.5 part of laccase. The total viable count content of Lactobacillus plantarum, lactobacillus acidophilus and Bacillus subtilis is greater than 2×10 10 CFU/g。
(c) Reviving the strain: pouring the silage bacterial enzyme into 1% syrup, fully dissolving, and standing for 2 hours at normal temperature, wherein the proportion of the silage bacterial enzyme to the 1% syrup is as follows: 1:2000 (w/v, g/mL).
(d) Preparing bacterial liquid: pouring the revived strain into 1000mL of 0.7% saline solution, stirring, and making the prepared strain solution incapable of overnight; wherein, the ratio of the revived strain to the saline solution is as follows: 2:1 (v/v, mL/mL).
(e) Spraying silage bacterial liquid into mulberry branches and leaves, and uniformly mixing to control the moisture content of the mixture to 60% so as to obtain silage mulberry branch and leaf mixture;
(f) Dividing and bundling (65 kg/bag) silage mulberry branch and leaf mixture, compacting (70 cm in specification: 40cm in specification: 30 cm), and sealing with double-layer bag;
(g) Fermenting at 37 deg.c for 35 days, and stopping anaerobic fermentation when pH value is 4.0-4.5 to obtain silage mulberry branch and leaf.
Detection examples
Sensory evaluation, analysis and measurement of moisture and main components of the products were performed on the silage mulberry branches and leaves obtained in the above preparation examples 1 to 3, and sensory evaluation criteria, moisture requirements at each stage and main component analysis assurance value criteria of the silage mulberry branches and leaves were preferably obtained as shown in tables 1 to 4.
TABLE 1 preparation of the test results for examples 1-3
TABLE 2 sensory evaluation criteria for silage mulberry branches and leaves
TABLE 3 moisture requirement of silaged mulberry branch and leaf products at all levels
| Product(s) | Special grade | First level | Second-level | Three stages |
| Moisture (%) is less than or equal to | 68.0 | 70.0 | 72.0 | 74.0 |
TABLE 4 analysis assurance value of Main ingredient of silage Mulberry branches and leaves
| Main component | Special grade | First level | Second-level | Three stages |
| Crude protein (%) is ≡ | 10.0 | 9.0 | 8.0 | 7.0 |
| Crude fiber (%) is less than or equal to | 44.0 | 45.0 | 46.0 | 47.0 |
| Coarse ash (%) is less than or equal to | 7.0 | 8.0 | 9.0 | 10.0 |
Note that: the content of each nutrient component is calculated on a dry matter basis.
Example 1
In this embodiment, the silage mulberry branch and leaf feed for beef cattle comprises the following raw materials in percentage by mass: 30% of silage mulberry branches and leaves, 30% of dry straw, 24.5% of corn, 2% of wheat bran, 5.8% of soybean meal, 2% of cottonseed cake, 3.5% of rapeseed meal, 0.3% of salt, 0.9% of stone powder, 1% of beef cattle premix (commercially available) and 100% in total. The silage mulberry branches and leaves are obtained by cutting and silaging mulberry branches and leaves.
The method for preparing silage mulberry branch and leaf feed for beef cattle by using the raw materials comprises the following steps:
(1) Crushing corn, wheat bran, bean pulp, cotton seed cake and rapeseed meal, sieving, mixing, stirring uniformly, and adding salt, stone powder and beef cattle premix;
(2) And (3) mixing the material obtained in the step (1) with silage mulberry branches and leaves and dry straws, uniformly stirring to obtain mixed feed, and tamping to obtain the silage mulberry feed for beef cattle. The silage mulberry branches and leaves in this example were obtained according to the preparation method according to preparation example 2.
Comparative example 1
In the comparative example, the silage mulberry branch and leaf feed for beef cattle comprises the following raw materials in percentage by mass: 30% of silage corn (commercially available), 30% of dry straw, 24.5% of corn, 2% of wheat bran, 5.8% of soybean meal, 2% of cottonseed cake, 3.5% of rapeseed meal, 0.3% of salt, 0.9% of stone powder, 1% of beef cattle premix and 100% of total.
The preparation method of the silage mulberry branch and leaf feed for beef cattle comprises the following steps:
(1) Crushing corn, wheat bran, bean pulp, cotton seed cake and rapeseed meal, sieving, mixing, stirring uniformly, and adding salt, stone powder and beef cattle premix;
(2) And (3) mixing the material obtained in the step (1) with silage corns and dry straws, uniformly stirring to obtain mixed feed, and tamping to obtain the feed of the comparative example.
Beef cattle were bred with silage mulberry feed of example 1 and conventional feed of comparative example 1 according to conventional methods, 16 healthy black cattle of normal growth and development were selected for the test, average age was 14 (+1.5) months, initial weight was 272kg (+ -50 kg), test animals were divided into 2 groups according to the principle of complete randomization, and each group was randomly subjected to a diet treatment, 8 bulls were bred by full-term bolting. And (3) expelling insects, marking ear marks and adapting to test daily ration for the test cattle in the pre-feeding period. Day 1, day 30 and day 60 of the positive feeding period were weighed, and the average daily gain, average daily feed intake and feed weight ratio were calculated, and the results are shown in table 5. The two groups of beef cattle were manure-like collected beginning 5 days before the end of the formal test, and the apparent digestibility of dry matter, crude protein, crude fat, crude ash, neutral washing fiber and acid washing fiber was measured, and the results are shown in table 6.
Table 5 results of measuring growth performance of Hei Bo in Xiang
Table 6 apparent digestibility test results of black cattle nutrient in Hunan province
The test results show that the preparation method can improve the digestibility of crude beef cattle proteins, crude ash, neutral washing fibers and acidic washing fibers under the condition that the production performance of beef cattle is not reduced.
The foregoing is merely illustrative of the preferred embodiments of this invention and it will be appreciated by those skilled in the art that variations and modifications may be made without departing from the principles of the invention, and it is intended to cover all such variations and modifications as fall within the true scope of the invention.
Claims (10)
1. The preparation method of the silage mulberry branches and leaves is characterized by comprising the following steps:
(a) Harvesting when the feed mulberry grows to 80-100 cm high or 30-45 days, and cutting mulberry branches and leaves into 2-3 cm after harvesting;
(b) Preparing raw materials of silage bacterial enzyme: the silage bacterial enzyme agent comprises the following raw materials in parts by mass: 2-4 parts of lactobacillus plantarum, 2-4 parts of lactobacillus acidophilus, 2-3 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5-1 part of xylanase and 0.5-1 part of laccase;
(c) Reviving the strain: pouring the silage bacterial enzyme into 1% syrup, fully dissolving, and standing for 1-2 h at normal temperature, wherein the proportion of the silage bacterial enzyme to the 1% syrup is as follows: 1:2000 (w/v, g/mL);
(d) Preparing silage bacterial liquid: pouring the revived strain into 0.7% -1.0% saline solution, uniformly stirring, and preparing the prepared bacterial solution which cannot be used overnight; wherein, the ratio of the revived strain to the saline solution is as follows: 2:1 (v/v, mL/mL);
(e) Spraying silage bacterial liquid into mulberry branches and leaves, and uniformly mixing to control the moisture content of the mixture to be 60% -70% so as to obtain silage mulberry branch and leaf mixture;
(f) Dividing, bundling and profiling silage mulberry branch and leaf mixture, and sleeving a double-layer bag for sealing;
(g) Fermenting at 35-37deg.C for 30-40 days, and stopping anaerobic fermentation when pH value is 4.0-4.5 to obtain silage mulberry branch and leaf.
2. The preparation method of silage mulberry branches and leaves according to claim 1, wherein the silage bacterial enzyme agent comprises the following raw materials in parts by mass: 3 parts of lactobacillus plantarum, 3 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5 part of xylanase and 0.5 part of laccase.
3. The preparation method of silage mulberry branches and leaves according to claim 1, wherein the silage bacterial enzyme agent comprises the following raw materials in parts by mass: 2 parts of lactobacillus plantarum, 4 parts of lactobacillus acidophilus, 2 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 0.5 part of xylanase and 1 part of laccase.
4. The preparation method of silage mulberry branches and leaves according to claim 1, wherein the silage bacterial enzyme agent comprises the following raw materials in parts by mass: 4 parts of lactobacillus plantarum, 2 parts of lactobacillus acidophilus, 3 parts of bacillus subtilis, 1 part of cellulase, 1 part of hemicellulase, 1 part of amylase, 1 part of xylanase and 0.5 part of laccase.
5. The method for preparing silage mulberry branches and leaves according to any one of claims 1 to 4, wherein the total viable count content of lactobacillus plantarum, lactobacillus acidophilus and bacillus subtilis in the silage bacterial agent is more than 2 x 10 10 CFU/g。
6. The method for preparing silage mulberry branches and leaves according to claim 5, wherein in the step (f), the silage mulberry branch and leaf mixture is divided and bundled into 65 kg/bag, and the compression specification is 70cm by 40cm by 30cm.
7. Silage mulberry branch and leaf prepared by the method of any one of claims 1-6.
8. The silage mulberry branch and leaf feed for beef cattle is characterized by comprising the following components in percentage by mass: the silage mulberry branch and leaf 30-35%, dried straw 30-35%, corn 24-25%, wheat bran 2-3%, soybean meal 5-6%, cotton seed cake 2-3%, rapeseed meal 3-4%, salt 0.3%, stone powder 0.9%, premix 1% and total 100%.
9. The silage mulberry twig and leaf feed for beef cattle of claim 8, which is characterized by comprising the following components in percentage by mass: the silage mulberry branch and leaf 30%, dried straw 30%, corn 24.5%, wheat bran 2%, soybean meal 5.8%, cotton seed cake 2%, rapeseed meal 3.5%, salt 0.3%, stone powder 0.9%, beef cattle premix 1% and total 100% of claim 7.
10. The method for preparing silage mulberry branch and leaf feed for beef cattle according to claim 8 or 9, comprising the steps of:
(1) Crushing corn, wheat bran, bean pulp, cotton seed cake and rapeseed meal, sieving, mixing, stirring uniformly, and adding salt, stone powder and beef cattle premix;
(2) And (3) mixing the material obtained in the step (1) with the silage mulberry branches and leaves and the dry straws, uniformly stirring to obtain mixed feed, and tamping to obtain the silage mulberry feed for beef cattle.
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