KR102461785B1 - Composition comprising NueDongChungHaCho(Paecilomyces tenuipes) alcohol extract for preventing or treating inflammatory disease - Google Patents

Abstract

The present invention relates to a composition for preventing, treating or improving an inflammatory disease comprising an alcohol extract of Cordyceps Cordyceps Cordyceps. The alcohol extract of Cordyceps Cordyceps Cordyceps according to the present invention has excellent anti-inflammatory effects, such as inhibiting NO and IL-1β production, and thus can be usefully used for the prevention, improvement or treatment of inflammatory diseases.

Description

TECHNICAL FIELD [0001] Composition comprising NueDongChungHaCho(Paecilomyces tenuipes) alcohol extract for preventing or treating inflammatory disease

The present invention relates to a composition for preventing, treating or improving an inflammatory disease comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

In modern society, the average lifespan of human beings has increased due to the development of medicine, and thus we are entering an aging society. Recently, along with the well-being craze, awareness of natural ingredients derived from plants is increasing. In the case of herbal medicines derived from all parts of medicinal plants, such as roots, fruits, flower bark, stems, leaves, and seeds of various plants, the safety and efficacy proven through prescriptions passed down from the past have been recognized. , bio food, oriental medicine cosmetics, etc. are being used in a variety of products made from herbal medicines. In addition, many studies are being conducted on functional materials such as pharmaceuticals and cosmetics using natural products that are friendly to the human body and have low side effects.

The inflammatory response is a biological defense response caused by various factors, such as infection by a pathogen or tissue damage, and performs an initial protective action to limit damage only to the site of infection or damage. In most cases, this inflammatory response leads to removal of pathogenic factors using components of innate immunity and induction of specific adaptive immunity (Hawiger J., Innate Immunity and Inflammation: A Transcriptional Paradigm. Immunologic Research. 23, pp. 99-109, 2001). Redness, edema, fever, pain, etc., which are known characteristics accompanying inflammation, are increased local blood flow and decreased local blood flow velocity and increased vascular permeability due to the expansion of blood vessels due to the action of inflammatory mediators and cytokines at the site of inflammation. The result of successive immune responses such as increased extravasation of plasma components according to (Gallo RL, Murakami M, Takaaki O, Zaiou M., Biology and clinical relevance of naturally occurring antimicrobial peptides. J. Allergy. Clin. Immunol. 110, pp.823-831. 2002; Graeme B. Ryan, MB, and Guido M., Acute Inflammation. American Journal of Pathology. 86(1), pp.185-274, 1977). Histamine produced by some cells as a response to tissue damage, and low-molecular-weight peptide kinin that exists in an inactive state in the blood and is activated by tissue damage also contribute to inflammation-related vasodilation and increased vascular permeability (Yamaki K, Thorlacius H, Xie X, Lindbom L, Hedqvist P, Raud J., Characteristics of histamineinduced leukocyte rolling in the undisturbed microcirculation of the rat mesentry. British J. Pharmacol. 123, pp. 390-399, 1998; Brocklehurst WE, Role of Kinins and Prostaglandins in Inflammation. Proc. Roy. Soc. Med. 64, pp. 4-6, 1971).

The inflammatory response at the site of infection is initiated by the response of macrophages to pathogens. Reactive reactive oxygen species and reactive nitrogen species produced by macrophages activated by pathogens (eg NO), prostaglandins, inflammatory mediators such as leukotriene, and proinflammatory agents such as TNF-α, IL-6 and IL-8 Cytokines are known to be involved in the inflammatory response (Renauld JC, New insights into the role of cytokines in asthma. J. Clin. Pathol. 54, pp.577-589, 2001; Blake GJ, Ridker PM, Tumor necrosis). factor-α, inflammatory biomarkers, and atherogenesis (Eur. Heart J. 23, pp.345347, 2002).

Among the drugs developed so far, the drug with the strongest anti-inflammatory action is a steroid preparation. However, with long-term use, steroids have a number of causes, including a round, multi-blooded face, fluid retention, adrenal suppression, increased susceptibility to infection, and other psychoses, cataracts, glaucoma, peptic ulcers, delayed wound healing, and reactivation of early infections. There are side effects.

On the other hand, Cordyceps Cordyceps is a name given because it stays in the form of an insect in winter and turns into a mushroom in summer. It means the entire drying of Snow Cordyceps Cordyceps. However, to date, the anti-inflammatory activity of Cordyceps Cordyceps silkworm has not been elucidated.

Accordingly, the present inventors conducted research to develop a new drug that is not toxic to the body, has fewer side effects, and has excellent anti-inflammatory effects. By confirming that there is an effect, the present invention was completed.

Accordingly, an object of the present invention is to provide a health functional food and food composition for the prevention or improvement of inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps silkworm.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps.

Another object of the present invention is to provide an external preparation for skin and a cosmetic composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

Another object of the present invention is 1) freeze-drying the Cordyceps Cordyceps silkworm; And 2) mixing the dried Cordyceps Cordyceps Cordyceps and alcohol in a weight ratio of 1:5 to 1:15 to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps; of a composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps. To provide a manufacturing method.

In order to achieve the above object, the present invention provides a health functional food composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps.

In addition, the present invention provides a food composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps.

In addition, the present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps.

In addition, the present invention provides a composition for external application for skin for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

In addition, the present invention provides a cosmetic composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps silkworm.

In addition, the present invention comprises the steps of: 1) freeze-drying Cordyceps Cordyceps; And 2) mixing the dried Cordyceps Cordyceps Cordyceps and alcohol in a weight ratio of 1:5 to 1:15 to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps; of a composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps. A manufacturing method is provided.

The alcohol extract of Cordyceps Cordyceps Cordyceps according to the present invention has excellent anti-inflammatory effects, such as inhibiting NO and IL-1β production, and thus can be usefully used for the prevention, improvement or treatment of inflammatory diseases.

1 is a diagram showing the effect of an alcohol extract of Cordyceps Cordyceps Cordyceps on the NO production induced by LPS.
2 is a diagram showing the effect of an alcohol extract of Cordyceps Cordyceps silkworm on the production of IL-1β induced by LPS.
Figure 3 is a diagram showing a comparison of the antioxidant activity of the silkworm cordyceps cord alcohol extract and red ginseng.
4 is a diagram showing a comparison of the polyphenol content of an alcohol extract of Cordyceps Cordyceps silkworm and red ginseng.

The present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps silkworm. In addition, the present invention provides a method for preparing a composition for topical application for skin, a cosmetic composition, a health functional food composition, a food composition, and a composition for preventing or treating an inflammatory disease comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

Hereinafter, the present invention will be described in more detail.

In the present invention, Cordyceps Cordyceps silkworm may refer to the entire drying of Cordyceps Cordyceps Cordyceps, which is formed by infecting the larvae (silkworm) of the silkworm moth or insect silkworm moth, including the entire Cordyceps Cordyceps that is formed by infecting the silkworm. could be a concept.

In the present invention, "extract" is an extract and fraction obtained by extraction treatment of Cordyceps Cordyceps Cordyceps, their dilution, concentrate, dried product obtained by drying the extract, a prepared or purified product of the extract, or a mixture thereof, etc., It includes extracts of all formulations that can be formed using the extract itself and the extract.

The alcohol extract of Cordyceps Cordyceps Cordyceps of the present invention is prepared by adding alcohol in a weight ratio of 1:5 to 1:15 to the dried Silkworm Cordyceps Cordyceps powder to be completely immersed, preferably by adding a solvent in a weight ratio of 1:8 to 1:12 It may be prepared, and more preferably, it may be prepared by adding a solvent 10 times the weight of the Cordyceps Cordyceps powder, but is not limited thereto. As the extraction method, extraction methods such as hot water extraction, cold extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be used. The extraction temperature may be a temperature of 40 °C to 80 °C, preferably 45 °C to 55 °C, more preferably 50 °C, but is not limited thereto. The extraction time may be extracted for 30 minutes to 3 hours, more preferably extracted for 30 minutes to 2 hours, and even more preferably extracted for 1 hour, but is not limited thereto. The alcohol as the extraction solvent may be a lower alcohol having 1 to 4 carbon atoms, and specifically may be at least one selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol, preferably ethanol. The ethanol may be 30 to 100% (v/v) ethanol, preferably 70% (v/v) ethanol, but is not limited thereto.

Particularly preferably, the alcohol extract of Cordyceps Cordyceps Cordyceps of the present invention may be extracted for 1 hour at a temperature of 50° C. using ethanol as a solvent.

Since the alcohol extract of Cordyceps Cordyceps Cordyceps of the present invention has excellent anti-inflammatory effects such as suppressing NO and IL-1β production without having toxicity in vivo, it is used in various fields such as pharmaceuticals, cosmetics, health functional foods, foods, and various supplements. It can be useful.

The present invention provides a pharmaceutical composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

In the present invention, the pharmaceutical composition for preventing or treating inflammatory diseases may specifically prevent or treat inflammatory diseases by inhibiting the expression of inflammatory factors and inflammatory cytokines, such as inhibiting NO and IL-1β production.

The inflammatory disease refers to diseases whose main lesion is inflammation, and is not limited thereto, but allergic diseases including, but not limited to, allergic asthma, allergic rhinitis, allergic mucositis, urticaria and anaphylax; Myopathy including systemic sclerosis, dermatomyositis and inclusion body myositis, arthritis, atopic dermatitis, psoriasis, asthma, multiple sclerosis, sepsis, polychondritis, scleroderma, eczema, gout, periodontitis disease, Behcet's syndrome, edema, vasculitis, Kawasaki disease, diabetic retinitis, autoimmune pancreatitis, vasculitis, glomerulonephritis, acute and chronic bronchitis and influenza infection.

In the present invention, the pharmaceutical composition may be formulated and used in various forms according to a conventional method. For example, it may be formulated in oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, and syrups, and may be formulated in the form of external preparations, suppositories, and sterile injection solutions.

The pharmaceutical composition of the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the active ingredients for administration. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like. it's not going to be The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like, in addition to the above components.

The dosage of the pharmaceutical composition of the present invention may vary depending on the formulation method, administration method, administration time and/or route of administration of the pharmaceutical composition, and the type and degree of response to be achieved by administration of the pharmaceutical composition. , the type of subject to be administered, age, weight, general health status, symptoms or severity of disease, sex, diet, excretion, components of drugs or other compositions used together with the subject at the same time or at different times, including several factors and similar factors well known in the pharmaceutical field, and those of ordinary skill in the art can easily determine and prescribe an effective dosage for the desired treatment.

The dosage of the pharmaceutical composition of the present invention may be, for example, 1 mg/kg to 1,000 mg/kg per day, but the dosage is not intended to limit the scope of the present invention in any way.

The route and mode of administration of the pharmaceutical composition of the present invention may be each independent, and the method is not particularly limited, and any route and mode of administration as long as the pharmaceutical composition can reach the desired site. can follow

The pharmaceutical composition may be administered by oral administration or parenteral administration. The parenteral administration method includes, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration, or subcutaneous administration, and methods of applying, spraying, or inhaling the pharmaceutical composition to the diseased site may also be used. However, it is not limited thereto.

The present invention provides a composition for external application for skin for preventing or improving inflammatory diseases, comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

In addition, the present invention provides a cosmetic composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps silkworm. Specifically, the cosmetic composition may be a cosmetic composition for inhibiting or alleviating inflammation.

The skin external application or cosmetic composition may be formulated as an ointment, a cream, a gel, a lotion, a liquid, a dressing, a patch, a blister, a tape, an aerosol, an external acid, and a spray.

In the composition of the present invention, any component generally used in external preparations for skin or cosmetic compositions may be used.

For example, in the case of ointments, creams, gels and lotions, white petrolatum (Vaseline), yellow petrolatum, lanolin, refined beeswax, cetanol, stearyl alcohol, stearic acid, hydrogenated oil, hydrocarbon gel, polyethylene glycol, bases such as liquid paraffin and squalane; Oleic acid, isopropyl myristate, glycerol triisooctanoate, crotamiton, diethyl sebacate, diisopropyl sebacate, diisopropyl adipate, hexylaulate, fatty acids, fatty acid esters, vegetable oils, fatty alcohols and solvents or stabilizers such as alcohols; antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; preservatives such as p-hydroxybenzoate; curbs such as glycerin, propylene glycol and sodium hyaluronate; surfactants such as polyoxyethylene derivatives, glycerol fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters and lecithin; thickeners such as carboxyvinylpolymer, xanthan gum, carboxymethyl cellulose and sodium carboxymethyl cellulose, hydroxypropyl cellulose and hydroxypropylmethyl cellulose; stabilizers; preservatives; adsorption promoters; and other suitable fillers may be added.

In the case of the blistering agent, a pressure-sensitive adhesive such as polyacrylic acid and polyacrylic acid copolymer; crosslinking agents such as aluminum sulfate, aluminum potassium sulfate, aluminum chloride, magnesium aluminometasilicate and dihydroxyaluminum aminoacetate; thickeners such as sodium polyacrylate, polyvinyl alcohol, polyvinylpyrrolidone, gelatin, sodium alginate, carboxymethylcellulose, sodium carboxymethyl cellulose, hydroxypropyl cellulose and hydroxypropylmethyl cellulose; polyhydric alcohols such as glycerin, polyethylene glycol (macrogol), polyethylene glycol and 1,3-butanediol; surfactants such as polyoxyethylene derivatives; flavorings such as l-menthol; preservatives such as p-hydroxybenzoate; Purified water; and other suitable fillers may be added.

In the case of the tape agent, an adhesive such as a styrene-isoprene-styrene block copolymer and an acrylate resin; adhesive resins such as acrylic saturated hydrocarbon resins, hydrogenated rosine resins and terpene resins; softeners such as liquid gums and liquid paraffin; antioxidants such as dibutylhydroxytoluene; polyhydric alcohols such as polyethylene glycol; adsorption promoters such as oleic acid; surfactants such as polyoxyethylene derivatives; and other suitable fillers may be added. In addition, a water-absorbing polymer such as sodium polyacrylate and polyvinyl alcohol and a small amount of purified water may be added to the preparation of the water-containing tape agent.

For aerosols, bases such as white petrolatum (Vaseline), yellow petrolatum, lanolin, refined beeswax, cetanol, stearyl alcohol, stearic acid, hydrogenated oil, hydrocarbon gel, polyethylene glycol, liquid paraffin and squalane; Oleic acid, isopropyl myristate, glycerol triisooctanoate, crotamiton, diethyl sebacate, diisopropyl sebacate, isopropyl adipate, hexyl laurate, fatty acids, fatty acid esters, vegetable oils, fatty alcohols and alcohols solvents or stabilizers such as; antioxidants such as tocopherol derivatives, L-ascorbic acid, dibutylhydroxytoluene and butylhydroxyanisole; preservatives such as p-hydroxybenzoate; curbs such as glycerin, propylene glycol and sodium hyaluronate; Surfactants such as polyoxyethylene derivatives, glycerol fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters and lecithin Carboxyvinyl polymers such as those used in ointments, creams, gels or lotions, xanthan gum, carboxymethyl thickeners such as cellulose and sodium carboxymethyl cellulose, hydroxypropyl cellulose and hydroxypropylmethyl cellulose; stabilizers; buffer; sweetener suspending agent; emulsifiers; Condiment; antiseptic; Adsorption promoters and other suitable fillers may be added.

In the case of the external powder, fillers such as potato starch, rice starch, corn starch, talc and zinc oxide, and other suitable additives may be added.

The skin external application or cosmetic composition of the present invention may be prepared by blending various known ingredients to be formulated in a composition applied to the skin or mucous membrane, and according to a well-known method for preparing an external preparation for skin or cosmetic composition, an appropriate base as necessary Together, it can be prepared by mixing each component well, and the preparation thus prepared is applied to the lesion as needed.

The alcohol extract of Cordyceps Cordyceps Cordyceps according to the present invention is included in an amount of 0.0001 to 50% by weight, preferably 1 to 30% by weight, based on the total weight of the skin external preparation or cosmetic composition of the present invention. If the content is less than 0.0001% by weight, the anti-inflammatory effect cannot be achieved, and when it exceeds 50% by weight, formulation is difficult and the feeling of use may be reduced.

The present invention provides a health functional food or food composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps silkworm.

In the present invention, “health functional food” refers to a food group or food composition that has added value to act and express the function of the food for a specific purpose using physical, biochemical, and bioengineering methods, etc. It refers to food that has been designed and processed to sufficiently express the body control functions related to disease prevention and recovery. For the purpose of the present invention, the health functional food is to promote anti-inflammatory activity, for example, refers to a health functional food for preventing and improving inflammatory diseases caused by the production of nitric oxide.

In the present invention, the food composition can be prepared by a method commonly used in the art, and during the preparation, it can be prepared by adding raw materials and components commonly added in the art. In addition, if the formulation of the food composition is also recognized as a food composition, it may be prepared without limitation.

Foods according to the present invention include, for example, various foods, beverages, gum, tea, vitamin complexes, functional foods, and the like. In addition, food includes special nutritional food (eg, formula milk, infant food, etc.), processed meat products, fish meat products, tofu, jelly, noodles (eg, ramen, noodles, etc.), breads, health supplements, seasoned foods (eg, soy sauce). , soybean paste, red pepper paste, mixed paste, etc.), sauces, confectionery (eg snacks), candy, chocolate, gum, ice cream, dairy products (eg fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi) , pickles, etc.), beverages (eg, fruit beverages, vegetable beverages, soy milk, fermented beverages, etc.), natural seasonings (eg, ramen soup, etc.), food additives, etc., but are not limited thereto. The food, beverage or food additive may be prepared by a conventional manufacturing method.

When the composition of the present invention is used as a health functional food additive, the composition may be added as it is or may be used together with other health functional food ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use. In general, in the production of food or beverage, the composition of the present invention may be added in an amount of preferably 50 parts by weight or less, more preferably 25 parts by weight or less, based on the raw material. However, in the case of long-term intake for the purpose of health control and hygiene, the amount may be less than the above range, and since there is no problem in terms of stability, the active ingredient may be used in an amount above the above range.

The food or health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates as an additional component, like a conventional food composition, in addition to containing an alcohol extract of Cordyceps Cordyceps Cordyceps as an active ingredient. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose and the like; and polysaccharides such as conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, erythritol and the like. The above-mentioned flavoring agent can advantageously use natural flavoring agent (Taumatine), stevia extract (eg rebaudioside A, glycyrrhizine, etc.) and synthetic flavoring agent (saccharin, aspartame, etc.).

In addition, the food composition contains various nutrients, vitamins, minerals (electrolytes), synthetic and natural flavoring agents, coloring agents and thickeners (cheese, chocolate, etc.), pectic acid and salts thereof, in addition to the alcohol extract of Cordyceps Cordyceps. , alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the food composition of the present invention may contain natural fruit juice and pulp for the production of fruit juice beverages and vegetable beverages.

The present invention also provides a method for preparing a composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps.

More specifically, 1) freeze-drying the Cordyceps Cordyceps; And 2) mixing the dried Cordyceps Cordyceps Cordyceps and alcohol in a weight ratio of 1:5 to 1:15 to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps; of a composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps. A manufacturing method is provided.

In the present invention, the drying in step 1) may be performed by freeze-drying in order to achieve the purpose of preparing the Cordyceps Cordyceps powder. The freeze-drying can be obtained by freezing the collected Cordyceps Cordyceps Cordyceps, thawing at room temperature, sterilizing it, and drying it in a freeze dryer at -35 to -45° C. for 24 to 72 hours. The dried freeze-dried product may be extracted after powdering.

The step of preparing the Cordyceps Cordyceps Cordyceps alcohol extract of step 2) of the present invention may be to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps silkworm by mixing the Cordyceps Cordyceps Cordyceps and alcohol in a weight ratio of 1:5 to 1:15, preferably with Cordyceps Cordyceps. Alcohol may be mixed in a weight ratio of 1:8 to 1:12, and more preferably, an alcohol of 10 times the weight of Cordyceps Cordyceps Cordyceps may be mixed to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps, but is not limited thereto.

In addition, the extraction temperature of step 2) of the present invention may be a temperature of 40 °C to 80 °C, preferably 45 °C to 55 °C, more preferably 50 °C, but is not limited thereto. The extraction time may be extracted for 30 minutes to 3 hours, more preferably extracted for 30 minutes to 2 hours, and even more preferably extracted for 1 hour, but is not limited thereto.

The alcohol as the extraction solvent may be a lower alcohol having 1 to 4 carbon atoms, and specifically, may be at least one selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol and isobutanol, preferably ethanol. The ethanol may be 30 to 100% (v/v) ethanol, preferably 70% (v/v) ethanol, but is not limited thereto.

Hereinafter, the content overlapping with the above-described content is omitted in order to avoid the complexity of the description, and the overlapped content may be equally applied.

Hereinafter, the present invention will be described in detail by way of Examples. The following examples and experimental examples are only for illustrating the present invention, but the present invention is not limited by the following examples.

Example 1. Preparation of alcohol extract of Cordyceps Cordyceps silkworm and preparation of analysis samples

The sample of the present invention was used by collecting Snow Flower Silkworm Cordyceps Cordyceps produced in Yeongdeok Farm in Gyeongbuk. The collected Cordyceps Cordyceps Cordyceps was prepared by freeze-drying using a freeze dryer and used in the following Examples. A freeze-dried Cordyceps Cordyceps and ethanol (70% (v/v)) 10 times the weight of the freeze-dried Cordyceps Cordyceps Cordyceps was prepared to prepare an alcohol extract of Cordyceps Cordyceps. The extraction was carried out at a temperature of 50° C. for 1 hour, and then the supernatant of the obtained extract was separated and freeze-dried to prepare an alcohol extract of Cordyceps Cordyceps Cordyceps.

In the present invention, RAW264.7 cells, a mouse-derived macrophage cell line, were purchased from the Korean Cell Line Bank (Seoul, Korea) and used for cell experiments.

Example 2. Confirmation of the anti-inflammatory effect of Cordyceps silkworm alcohol extract

Example 2.1 Preparation of Comparative Control

Red ginseng used in the comparative experiment was provided by Cheong Kwan Jang and used, and the Cordyceps Cordyceps water extract was prepared by mixing freeze-dried Cordyceps Cordyceps and water 10 times the weight of the freeze-dried Cordyceps Cordyceps. Extraction was performed at 100° C. for 2 hours to prepare a Cordyceps Cordyceps water extract. Since red ginseng among natural products is known as a substance exhibiting an anti-inflammatory effect, the anti-inflammatory effect was confirmed by using this as a comparative control group.

Cultured RAW264.7 cells were treated with LPS to induce inflammation, and the Cordyceps Cordyceps Cordyceps alcohol extract and water extract and red ginseng for comparative experiments were added to each medium and cultured for 24 hours. The inhibition of NO (nitric oxide) and IL-1β production was confirmed according to the manufacturer's recommended method. For the analysis of NO production inhibition, griess reagent was additionally mixed with the culture supernatant, and absorbance was measured at 548 nm after 15 minutes.

Example 2.2 Confirmation of inhibition of NO (nitric oxicd) production of Cordyceps silkworm alcohol extract

In order to confirm the NO production inhibitory effect of the alcohol extract of Cordyceps Cordyceps Cordyceps prepared in Example 1, the degree of inhibition of NO production was analyzed. NO is involved in physiological processes such as blood coagulation, regulation of blood pressure and neurotransmission functions, and plays an important role in maintaining cellular functions depending on the concentration. It is known to cause cell death, produce harmful substances, play an important role in the formation and progression of cancer, accumulate harmful oxidative substances in cells, damage DNA, and cause natural cell death. The NO production amount was confirmed by the method of Example 3.1, and the results are shown in FIG. 1 .

As shown in FIG. 1 , the LPS-treated group showed a higher amount of NO production compared to the LPS-untreated group, and it was confirmed that the Cordyceps Cordyceps alcohol extract effectively inhibited the increase in NO induced in the extract-untreated control group at all concentrations. In addition, the alcohol extract of Cordyceps Cordyceps from the same concentration of red ginseng and Cordyceps Cordyceps water extract was more effective in inhibiting NO production, and the NO production inhibitory ability was increased in a concentration-dependent manner. The above results are results showing that the alcohol extract of Cordyceps Cordyceps Cordyceps exhibits an anti-inflammatory effect by effectively achieving inhibition of NO production, which is known to play an important role in inducing inflammation.

Example 2.3 Confirmation of inhibition of IL-1β production of Cordyceps silkworm alcohol extract

The IL-1β production inhibitory effect of the alcohol extract of Cordyceps Cordyceps Cordyceps prepared in Example 1 was confirmed. IL-1β is a cytokine involved in the inflammatory response, and an increase in IL-1β is known to cause a number of auto-inflammatory diseases. The amount of IL-1β production was confirmed by the method of Example 3.1, and the results are shown in FIG. 2 .

As shown in FIG. 2 , the LPS-treated group showed a higher amount of IL-1β production compared to the LPS-untreated group, and the alcohol extract of Cordyceps Cordyceps Cordyceps increased IL-1β induced in the extract-untreated control group at all concentrations (μg/ml). It was confirmed that it was effectively inhibited. In addition, it was confirmed that the alcohol extract of Cordyceps Cordyceps cordyceps cordyxinosaurus cordyceps cordyxinosaurus cordyceps Cordyceps Cordyceps Cordyceps Cordyceps Cordyceps Cordycoma from IL-1β was more effective than red ginseng at the concentrations of 500 (μg/ml) and 1000 (μg/ml) at the same concentration. It was confirmed that the production inhibitory ability was increased. In particular, the alcohol extract of Cordyceps Cordyceps silkworm was superior to the water extract of Cordyceps Cordyceps silkworm in inhibiting IL-1β production at all concentrations.

The above results are results showing that the alcohol extract of Cordyceps Cordyceps Cordyceps exhibits an anti-inflammatory effect by effectively achieving inhibition of IL-1β production, which is known to play an important role in inducing inflammation.

Comparative Example 1. Comparison of antioxidant activity and polyphenol content of alcohol extract of Cordyceps Cordyceps silkworm and red ginseng

Comparison of antioxidant activity and polyphenol content between the alcohol extract of Cordyceps Cordyceps Cordyceps and red ginseng was performed, and the results are shown in FIG. 3 . The antioxidant activity was confirmed by the ABTS method, and the red ginseng used in the comparative experiment was provided by Cheong Kwan Jang.

As shown in FIG. 3 , it was confirmed that the alcohol extract of Cordyceps Cordyceps Cordyceps removed more than 50% of active oxygen and showed higher antioxidant activity than red ginseng. In addition, as shown in FIG. 4 , it was confirmed that the polyphenol content of the alcohol extract of Cordyceps Cordyceps Cordyceps was also higher than that of red ginseng.

These results show that the alcohol extract of Cordyceps Cordyceps Cordyceps of the present invention exhibits high active oxygen removal and polyphenol content compared to red ginseng, which exhibits strong antioxidant and anti-inflammatory effects, and can achieve excellent anti-inflammatory effects.

Hereinafter, formulation examples of the composition of the present invention will be described, but the present invention is not intended to limit the present invention, but merely to describe in detail.

Formulation Example 1. Preparation of a pharmaceutical composition

1- 1. Preparation of powder

2 g of alcohol extract of Cordyceps Cordyceps silkworm

1 g lactose

The above ingredients were mixed and filled in an airtight cloth to prepare a powder.

1- 2. Preparation of tablets

Silkworm Cordyceps Cordyceps Alcohol Extract 100 mg

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, tablets were prepared by tableting according to a conventional method for manufacturing tablets.

1-3. manufacture of capsules

Silkworm Cordyceps Cordyceps Alcohol Extract 100 mg

100 mg cornstarch

Lactose 100 mg

2 mg magnesium stearate

After mixing the above ingredients, the capsules were prepared by filling in gelatin capsules according to a conventional manufacturing method of capsules.

Formulation Example 2. Preparation of composition for external application for skin/cosmetics

2-1. Manufacture of softening lotion (skin lotion)

Silkworm Cordyceps Cordyceps Alcohol Extract 0.5%

Beta-1,3-glucan 1.0%

Butylene Glycol 2.0 %

Propylene glycol 2.0 %

Carboxyvinyl Polymer 0.1 %

PEG-12 Nonylphenyl Ether 0.2 %

Polysorbate 80 0.4%

Ethanol 10.0%

Triethanolamine 0.1%

Preservatives, colors and fragrances

Purified water to 100 %

2-2. preparation of cream

Silkworm Cordyceps Cordyceps Alcohol Extract 1.0%

Beta-1,3-glucan 5.0%

beeswax 10.0%

Polysorbate 60 1.5%

Sebum 60 hydrogenated castor oil 2.0%

Sorbitan sesquioleate 0.5%

Liquid paraffin 10.0 %

Squalane 5.0%

Caprylic/Capric Triglycerides 5.0 %

Glycerin 5.0%

Butylene Glycol 3.0 %

Propylene glycol 3.0 %

Triethanolamine 0.2%

Preservatives, colors and fragrances

Purified water to 100 %

Claims (18)

As a health functional food composition for preventing or improving inflammatory diseases, comprising an alcohol extract of Cordyceps Cordyceps Cordyceps from 70% (v / v) ethanol as a solvent for 1 hour at 50 ° C.
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits NO and IL-1β production, and polyphenols and antioxidant activity are increased, health functional food composition for the prevention or improvement of inflammatory diseases. delete delete delete delete delete delete As a food composition for the prevention or improvement of inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps from 70% (v / v) ethanol as a solvent for 1 hour at 50 ° C.
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits NO and IL-1β production, and polyphenols and antioxidant activity is increased, food composition for the prevention or improvement of inflammatory diseases. A pharmaceutical composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps from 70% (v / v) ethanol as a solvent for 1 hour at 50 ° C.
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits NO and IL-1β production, and polyphenol and antioxidant activity is increased, a pharmaceutical composition for the prevention or treatment of inflammatory diseases. 10. The method of claim 9, wherein the inflammatory disease is allergic asthma, allergic rhinitis, allergic mucositis, urticaria and allergic diseases including anaphylax, systemic sclerosis, dermatomyositis and including Myopathy including inclusion body myositis, arthritis, atopic dermatitis, psoriasis, asthma, multiple sclerosis, sepsis, polychondritis, scleroderma, eczema, gout, periodontal disease, Behcet's syndrome, edema, vasculitis, Kawasaki disease, At least one selected from the group consisting of diabetic retinitis, autoimmune pancreatitis, vasculitis, glomerulonephritis, acute and chronic bronchitis and influenza infection, a pharmaceutical composition for preventing or treating inflammatory diseases. As a skin external composition for preventing or improving inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps from 70% (v / v) ethanol as a solvent for 1 hour at 50 ° C.
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits NO and IL-1β production, and polyphenol and antioxidant activity is increased, the composition for external application for the prevention or improvement of inflammatory diseases. As a cosmetic composition for the prevention or improvement of inflammatory diseases, comprising an alcohol extract of Cordyceps Cordyceps Cordyceps from 70% (v/v) ethanol as a solvent and extracted for 1 hour at a temperature of 50°C,
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits NO and IL-1β production, and polyphenol and antioxidant activity is increased, a cosmetic composition for the prevention or improvement of inflammatory diseases. 1) freeze-drying the Cordyceps Cordyceps Cordyceps; and
2) The dried Cordyceps Cordyceps Cordyceps and alcohol are mixed in a weight ratio of 1:5 to 1:15, and extracted with 70% (v/v) ethanol as a solvent at 50 ° C. for 1 hour to prepare an alcohol extract of Cordyceps Cordyceps. As a method for preparing a composition for preventing or treating inflammatory diseases comprising an alcohol extract of Cordyceps Cordyceps Cordyceps,
The alcohol extract of Cordyceps Cordyceps Cordyceps, inhibits the production of NO and IL-1β, and polyphenol and antioxidant activity is increased, characterized in that the composition for the prevention or treatment of inflammatory diseases.
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