KR102445679B1 - Antioxidant composition comprising silkworm pupa extract and red ginseng extract - Google Patents
Antioxidant composition comprising silkworm pupa extract and red ginseng extract Download PDFInfo
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- KR102445679B1 KR102445679B1 KR1020190141519A KR20190141519A KR102445679B1 KR 102445679 B1 KR102445679 B1 KR 102445679B1 KR 1020190141519 A KR1020190141519 A KR 1020190141519A KR 20190141519 A KR20190141519 A KR 20190141519A KR 102445679 B1 KR102445679 B1 KR 102445679B1
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- extract
- red ginseng
- silkworm pupa
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- ginseng extract
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Abstract
본 발명은 누에 번데기 및 홍삼추출물을 포함하는 항산화용 조성물에 관한 것으로서, 더욱 상세하게는 상기 누에 번데기 및 홍삼 추출물을 포함하는 항산화용 식품, 화장료, 약학적 조성물 또는 의약외품 조성물에 관한 것이다. 본 발명에 따른 누에 번데기 및 홍삼추출물은 폴리페놀 함량 증가 및 ABTS 라디칼, DPPH 라디칼 소거 활성을 나타내는 바, 항산화 목적의 의약품, 식품, 화장품, 의약외품 분야에 다양하게 활용될 수 있다. The present invention relates to an antioxidant composition comprising silkworm pupa and red ginseng extract, and more particularly, to an antioxidant food, cosmetic, pharmaceutical composition or quasi-drug composition comprising the silkworm pupa and red ginseng extract. The silkworm pupae and red ginseng extract according to the present invention increase the polyphenol content and exhibit ABTS radical and DPPH radical scavenging activity, and thus can be variously used in the fields of anti-oxidation drugs, food, cosmetics, and quasi-drugs.
Description
본 발명은 누에 번데기 및 홍삼추출물을 포함하는 항산화용 조성물에 관한 것으로서, 더욱 상세하게는 상기 누에 번데기 및 홍삼 추출물을 포함하는 항산화용 식품, 화장료, 약학적 조성물 또는 의약외품 조성물에 관한 것이다.The present invention relates to an antioxidant composition comprising silkworm pupa and red ginseng extract, and more particularly, to an antioxidant food, cosmetic, pharmaceutical composition or quasi-drug composition comprising the silkworm pupa and red ginseng extract.
활성 산소는 생체 외부로부터 유입되거나, 생체 내에서 발생하여 노화를 촉진시키거나, 암 발생의 원인이 되는 것으로 알려져 있다. 따라서 활성산소에 의한 산화적 손상을 억제하는 항산화 물질에 대한 연구가 활발히 진행되고 있으며, 대표적으로 페놀성 화합물, 플라보노이드, 토코페롤, 비타민 C, 셀레늄 등이 알려져 있다. 한편 최근 합성 물질인 화학 항산화제가 인체에 부작용을 나타내는 등 안전성에 대한 문제가 있어 새로운 천연 물질 항산화제에 대한 필요성이 대두되고 있다. 천연에 존재하는 항산화 물질은 인체에 적용에 적용할 경우 실질적으로 충분한 효과를 기대할 수 없다는 연구 결과들이 보고됨에 따라, 합성 화학물질을 대체한 새로운 천연 항산화제에 대한 필요성이 지속적으로 제기되고 있다.Active oxygen is known to be introduced from outside the living body, or generated in the living body to accelerate aging or cause cancer. Therefore, studies on antioxidants that inhibit oxidative damage caused by free radicals are being actively conducted, and representatively, phenolic compounds, flavonoids, tocopherols, vitamin C, selenium, and the like are known. On the other hand, the need for new natural antioxidants is emerging due to safety issues such as chemical antioxidants, which are synthetic substances, have side effects on the human body. As research results report that natural antioxidants cannot be expected to have practically sufficient effects when applied to the human body, the need for new natural antioxidants that replace synthetic chemicals is constantly being raised.
최근 자연 지향적이고 환경 친화적인 소비추세에 따라 화장품, 식품에 들어가는 유효성분도 식물 또는 동물 유래의 천연물의 기능성을 기반으로 하여 이들을 여러 가지 형태로 배합하여 사용되고 있으며, 근래에는 국내·외에서 소위, 웰빙의 욕구가 증가되어 건전한 식생활과 건강 및 질병 예방을 위한 관심이 주목되고 있는 실정이다. According to the recent nature-oriented and eco-friendly consumption trend, active ingredients in cosmetics and food are also mixed and used in various forms based on the functionality of natural products derived from plants or animals. There is a growing interest in healthy eating, health and disease prevention.
예로부터 무공해 생명체로 알려져 있는 누에 번데기는 최근에 분말 및 추출물을 이용하여 강정제뿐만 아니라 당뇨, 고혈압 등의 성인병 치료 및 면역기능 강화를 위한 기능성 식품으로 많이 이용되고 있다. 현재까지 천연물로서의 누에의 안전성과 임상적 효능은 익히 알려져 있으나 그 활성 기작은 아직까지 확실치 않다. 누에뿐만 아니라 천연물로서의 누에 번데기 그 자체도 부작용이 적고 다양한 성인병 관련 질환의 치료제로서의 효능이 연구되고 있고, 누에 번데기에 다양한 봄비콜, 히드록시헥사데카디엔, 봄비신 등의 단백질과 아르기닌 등의 아미노산 함량이 높아 다양한 효능을 보이는 것으로 예상되고 있다.Silkworm pupa, known as a non-polluting organism since ancient times, has recently been widely used as a functional food for strengthening immune function and treating adult diseases such as diabetes and high blood pressure as well as gangrene using powder and extract. Until now, the safety and clinical efficacy of silkworm as a natural product are well known, but the mechanism of its activity is not yet clear. Not only silkworms but also silkworm pupae as natural products have few side effects and their efficacy as a treatment for various adult disease-related diseases is being studied. This is expected to show various effects.
그러나 아직까지 누에 번데기를 주요 성분으로 항산화제로 활용하거나 다른 유용 작물과 혼합하여 항산화제로 활용하고자 하는 연구는 널리 보고된 바 없다. However, studies using silkworm pupa as a main component as an antioxidant or mixing it with other useful crops as an antioxidant have not been widely reported.
이에 본 발명자들은 천연물의 효능을 증진시키기 위한 방법을 연구한 결과, 누에 번데기 추출물 및 홍삼 추출물이 현저한 항산화 효과가 있음을 확인함으로써 본 발명을 완성하게 되었다.Accordingly, the present inventors have completed the present invention by confirming that the silkworm pupa extract and the red ginseng extract have significant antioxidant effects as a result of studying a method for enhancing the efficacy of natural products.
따라서 본 발명의 목적은, 누에 번데기 추출물 및 홍삼 추출물을 포함하는 항산화용 식품, 화장료, 약학적 조성물 및 의약외품 조성물을 제공하는 것이다.Accordingly, an object of the present invention is to provide an antioxidant food, cosmetic, pharmaceutical composition and quasi-drug composition comprising a silkworm pupa extract and a red ginseng extract.
상기 목적을 달성하기 위하여, 본 발명은 누에 번데기 추출물 및 홍삼 추출물을 포함하는 항산화용 식품 조성물을 제공한다.In order to achieve the above object, the present invention provides a food composition for antioxidants comprising a silkworm pupa extract and a red ginseng extract.
또한 본 발명은 누에 번데기 및 홍삼추출물을 포함하는 항산화용 화장료 조성물을 제공한다.In addition, the present invention provides a cosmetic composition for antioxidants comprising silkworm pupa and red ginseng extract.
또한 본 발명은 누에 번데기 및 홍삼추출물을 포함하는 항산화용 약학적 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for antioxidants comprising silkworm pupa and red ginseng extract.
또한 본 발명은 누에 번데기 및 홍삼추출물을 포함하는 항산화용 의약외품 조성물을 제공한다. In addition, the present invention provides a quasi-drug composition for antioxidants comprising silkworm pupa and red ginseng extract.
본 발명에 따른 누에 번데기 및 홍삼추출물은 폴리페놀 고함유에 따른 ABTS 라디칼, DPPH 라디칼 소거 활성을 나타내는 바, 항산화 목적의 의약품, 식품, 화장품, 의약외품 분야에 다양하게 활용될 수 있다. The silkworm pupae and red ginseng extract according to the present invention exhibit ABTS radical and DPPH radical scavenging activity according to the high polyphenol content, and thus can be variously used in the fields of anti-oxidation drugs, food, cosmetics, and quasi-drugs.
도 1은 추출 용매에 따른 누에 번데기 추출물의 폴리페놀 함량 측정 결과를 나타낸 도이다.
도 2는 추출 용매에 따른 누에 번데기 추출물의 ABTS 라디칼 소거 활성을 측정한 결과를 나타내는 도이다.
도 3은 추출 용매에 따른 누에 번데기 추출물의 DPPH 라디칼 소거 활성을 측정한 결과를 나타내는 도이다.
도 4는 추출용매에 따른 홍삼 추출물의 폴리페놀 함량 측정 결과를 나타낸 도이다.
도 5는 추출용매에 따른 홍삼 추출물의 DPPH 라디칼 소거 활성을 측정한 결과를 나타내는 도이다.
도 6은 추출 용매에 따른 홍삼 추출물의 ABTS 라디칼 소거 활성을 측정한 결과를 나타내는 도이다.
도 7은 추출 조건 및 혼합 조건에 따른 누에 번데기 추출물 및 홍삼 추출물의 DPPH 라디칼 소거 활성을 측정한 결과를 나타내는 도이다.
도 8 은 추출 조건 및 혼합 조건에 따른 누에 번데기 추출물 및 홍삼 추출물의 세포내 활성 산소종 억제 효과를 측정한 결과를 나타내는 도이다.1 is a view showing the measurement result of the polyphenol content of the silkworm pupa extract according to the extraction solvent.
2 is a diagram showing the results of measuring the ABTS radical scavenging activity of the silkworm pupa extract according to the extraction solvent.
3 is a diagram showing the results of measuring the DPPH radical scavenging activity of the silkworm pupa extract according to the extraction solvent.
4 is a diagram showing the measurement result of the polyphenol content of the red ginseng extract according to the extraction solvent.
5 is a diagram showing the results of measuring the DPPH radical scavenging activity of the red ginseng extract according to the extraction solvent.
6 is a diagram showing the results of measuring the ABTS radical scavenging activity of the red ginseng extract according to the extraction solvent.
7 is a diagram showing the results of measuring the DPPH radical scavenging activity of silkworm pupa extract and red ginseng extract according to extraction conditions and mixing conditions.
8 is a diagram showing the results of measuring the intracellular reactive oxygen species inhibitory effect of silkworm pupa extract and red ginseng extract according to extraction conditions and mixing conditions.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 양태에 따르면, 누에 번데기 추출물 및 홍삼 추출물을 포함하는 항산화용 식품 조성물, 화장료 조성물, 항산화용 약학적 조성물 및 의약외품 조성물을 제공한다.According to an aspect of the present invention, there is provided a food composition for antioxidant, a cosmetic composition, a pharmaceutical composition for antioxidant, and a quasi-drug composition comprising a silkworm pupa extract and a red ginseng extract.
본 발명에 있어서, “추출물”은 적절한 용매를 이용하여 누에 번데기 및 홍삼으로부터 추출한 것이며, 예를 들어 누에 번데기 및 홍삼의 조추출물, 극성용매 가용 추출물 또는 비극성용매 가용 추출물을 모두 포함한다. 또한, 상기 추출물은 추출, 분획 또는 정제의 각 단계에서 얻어지는 모든 추출액, 분획물, 그들의 희석액, 농축액 또는 건조물을 모두 포함하는 개념이다.In the present invention, "extract" is extracted from silkworm pupa and red ginseng using an appropriate solvent, and includes, for example, crude extracts of silkworm pupa and red ginseng, polar solvent-soluble extracts, or non-polar solvent-soluble extracts. In addition, the extract is a concept including all extracts, fractions, their dilutions, concentrates, or dried products obtained in each step of extraction, fractionation or purification.
본 발명의 조성물의 유효성분인 누에 번데기 및 홍삼추출물은 통상적인 추출 방법에 의해 얻을 수 있고 또는 시판되는 것을 구입하여 사용할 수도 있다.Silkworm pupa and red ginseng extract, which are active ingredients of the composition of the present invention, may be obtained by a conventional extraction method or may be purchased and used commercially.
상기 누에 번데기 및 홍삼추출물은 당업계에 공지된 추출, 분리 및 분획하는 방법을 사용하여 천연으로부터 추출, 분리 및 분획하여 수득한 것을 사용할 수 있다. The silkworm pupae and red ginseng extract may be obtained by extraction, separation and fractionation from nature using methods known in the art for extraction, separation and fractionation.
상기 누에 번데기 추출물 및 홍삼 추출물을 수득하기 위한 적절한 용매로는 물 또는 유기용매를 사용할 수 있으나, 메탄올(methanol), 에탄올(ethanol), 프로판올(propanol), 이소프로판올(isopropanol), 부탄올(butanol) 등의 탄소수 1 내지 4의 알코올 등을 단독으로 또는 2종 이상 혼합하여 사용할 수 있다. 바람직하게, 상기 용매로는 메탄올 또는 에탄올을 사용할 수 있고, 더욱 바람직하게는 에탄올을 사용할 수 있다. As a suitable solvent for obtaining the silkworm pupa extract and red ginseng extract, water or an organic solvent may be used, but methanol, ethanol, propanol, isopropanol, butanol, etc. Alcohols having 1 to 4 carbon atoms may be used alone or in combination of two or more. Preferably, methanol or ethanol may be used as the solvent, and more preferably ethanol may be used.
특히 본 발명의 누에 번데기 추출물은 탄소수 1 내지 4의 알코올을 이용하는 경우, 20 내지 50 % (v/v) 알코올을 이용하여 추출한 추출물일 수 있으며, 더욱 바람직하게는 25 내지 35 % (v/v)의 에탄올 추출물일 수 있다. 25 내지 35 % (v/v)의 에탄올 추출용매를 통해 추출된 누에 번데기 추출물은 누에 번데기 자체를 물 또는 탄소수 1 내지 4의 알코올로 추출한 추출물과 비교하여 항산화 효과가 우수할 뿐만 아니라, 특히 누에 번데기 수 추출물과 비교하더라도 현저히 우수한 항산화 효과를 나타낼 수 있다. In particular, the silkworm pupa extract of the present invention may be an extract extracted using 20 to 50% (v/v) alcohol, more preferably 25 to 35% (v/v) when using alcohol having 1 to 4 carbon atoms. It may be an ethanol extract of The silkworm pupa extract extracted through 25 to 35% (v/v) ethanol extraction solvent has excellent antioxidant effect compared to the extract obtained by extracting the silkworm pupa itself with water or alcohol having 1 to 4 carbon atoms, in particular, silkworm pupa Even compared to the water extract, it can exhibit a remarkably excellent antioxidant effect.
또한 본 발명의 홍삼 추출물은 탄소수 1 내지 4의 알코올을 이용하는 경우, 30 내지 60 % (v/v) 알코올을 이용하여 추출한 추출물 일 수 있으며, 더욱 바람직하게는 40 내지 60 % (v/v)의 에탄올 추출물일 수 있다. 40 내지 60 % (v/v)의 에탄올 추출용매를 통해 추출된 홍삼 추출물은 홍삼 수 추출물과 비교하여 항산화 효과가 우수할 뿐만 아니라, 특히 홍삼을 100 % (v/v) 에탄올로 추출한 추출물과 비교하더라도 현저히 우수한 항산화 효과를 나타낼 수 있다.In addition, when the red ginseng extract of the present invention uses alcohol having 1 to 4 carbon atoms, it may be an extract extracted using 30 to 60% (v/v) alcohol, and more preferably 40 to 60% (v/v) of alcohol. It may be an ethanol extract. The red ginseng extract extracted through 40 to 60% (v/v) ethanol extraction solvent not only has excellent antioxidant effect compared to the red ginseng water extract, but especially compared to the extract extracted with 100% (v/v) ethanol of red ginseng. However, it can exhibit a remarkably excellent antioxidant effect.
또한 본 발명은 항산화 효과를 증진시키기 위하여 누에 번데기 추출물 및 홍삼 추출물을 포함하고, 누에 번데기 추출물 및 홍삼 추출물을 항산화 목적으로 이용하는 경우, 홍삼 추출물 대비 누에 번데기 추출물이 더 많은 부피비로 포함되는 것이 바람직하다. 예컨대 바람직하게는 1:1 내지 3:1 부피비로 혼합될 수 있고, 이는 홍삼 추출물이 더 많은 부피비로 함유되는 혼합 추출물 대비 더욱 우수한 항산화 효과를 달성할 수 있다. 예컨대, 본 발명의 일 구현예에서는 누에 번데기 추출물 및 홍삼 추출물을 50:50, 75:25 으로 혼합한 실시예가 25:75 부피 혼합 추출물 대비 우수한 항산화 효과가 달성되는 것을 확인하였다. In addition, the present invention includes a silkworm pupa extract and red ginseng extract to enhance the antioxidant effect, and when the silkworm pupa extract and red ginseng extract are used for antioxidant purposes, it is preferable that the silkworm pupa extract is included in a larger volume ratio compared to the red ginseng extract. For example, it may be preferably mixed in a volume ratio of 1:1 to 3:1, which can achieve a better antioxidant effect than a mixed extract containing a red ginseng extract in a larger volume ratio. For example, in one embodiment of the present invention, it was confirmed that the example in which the silkworm pupa extract and the red ginseng extract were mixed at 50:50, 75:25 achieved an excellent antioxidant effect compared to the 25:75 volume mixed extract.
또한 본 발명은 상기와 같은 누에 번데기 추출물 및 홍삼 추출물의 혼합을 통해, 누에 번데기, 홍삼 추출물 단일 추출물과 대비하여 우수한 폴리페놀 함량, DPPH 자유 라디칼 소거능, 세포 내 활성 산소종 제거능을 확인하였고, 단일 추출물 대비 약 2배 이상의 활성 증진 효과를 확인하였다. In addition, the present invention confirmed the superior polyphenol content, DPPH free radical scavenging ability, and intracellular reactive oxygen species removal ability, compared to the single extract of silkworm pupae and red ginseng extract, through the mixing of the silkworm pupa extract and red ginseng extract as described above, and the single extract It was confirmed that the activity enhancing effect of about 2 times or more compared to the comparison.
본 발명에 있어, 누에 번데기 추출물 및 홍삼 추출물의 추출 온도는 30 내지 100℃인 것이 바람직하며, 12 내지 96시간, 바람직하게는 24 내지 72시간 동안 추출할 수 있다. 또한 추출방법으로는 열수추출법, 냉침추출법, 환류냉각추출법, 용매추출법, 수증기증류법, 초음파추출법, 용출법, 압착법 등의 다양한 방법이 사용될 수 있으나 이에 제한되지는 않고, 바람직하게는 열수추출법 또는 환류냉각추출법을 사용하는 것이다.In the present invention, the extraction temperature of the silkworm pupa extract and the red ginseng extract is preferably 30 to 100° C., and can be extracted for 12 to 96 hours, preferably 24 to 72 hours. In addition, as the extraction method, various methods such as hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, compression method, etc. may be used, but are not limited thereto, preferably hot water extraction method or reflux method Cold extraction method is used.
상기 누에 번데기 및 홍삼추출물은 당업계에서 알려진 통상의 방법으로 상온에서 냉침, 가열 및 여과하여 액상물을 얻을 수 있으며, 또는 추가로 용매를 증발, 분무건조 또는 동결건조 할 수도 있다. 또한 상기 누에 번데기 및 홍삼추출물은 감압 증류 및 동결 건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조될 수도 있다. 또한 상기 추출물 또는 분획물을 실리카겔 컬럼 크로마토그래피(silica gel column chromatography), 박층크로마토그래피(thin layer chromatography), 고성능 액체 크로마토그래피(high performance liquid chromatography) 등과 같은 다양한 크로마토그래피를 이용하여 추가로 정제된 분획으로도 얻을 수 있다.The silkworm pupae and red ginseng extract can be cooled at room temperature, heated and filtered by a conventional method known in the art to obtain a liquid, or the solvent may be further evaporated, spray-dried or freeze-dried. In addition, the silkworm pupae and the red ginseng extract may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying. In addition, the extract or fraction is further purified using various chromatography methods such as silica gel column chromatography, thin layer chromatography, high performance liquid chromatography, etc. can also be obtained
따라서 본 발명에서 사용되는 상기 누에 번데기 및 홍삼추출물은 추출, 분획 또는 정제의 각 단계에서 얻어지는 모든 추출물, 분획물 및 정제물, 그들의 희석액, 농축액 또는 건조물을 모두 포함하는 개념이다.Therefore, the silkworm pupae and red ginseng extract used in the present invention is a concept including all extracts, fractions and purified products obtained in each step of extraction, fractionation or purification, and their dilutions, concentrates or dried products.
본 발명의 일 실시예에 따르면, 상기 누에 번데기 추출물 및 홍삼추출물은 총 폴리페놀 함량이 우수하고, DPPH 및 ABTS 라디칼 소거 활성이 우수하다. 따라서, 본 발명의 누에 번데기 추출물 및 홍삼 추출물은 항산화 조성물로 유용하게 활용될 수 있다.According to an embodiment of the present invention, the silkworm pupa extract and red ginseng extract have excellent total polyphenol content and excellent DPPH and ABTS radical scavenging activity. Therefore, the silkworm pupa extract and red ginseng extract of the present invention can be usefully used as an antioxidant composition.
또한 본 발명의 누에 번데기 추출물 및 홍삼 추출물은 누에 번데기 및 홍삼을 혼합하여 동시에 추출한 것, 이를 각각 순차적으로 각각 추출하여 혼합한 것을 모두 포함한다. In addition, the silkworm pupa extract and red ginseng extract of the present invention include both those extracted at the same time by mixing the silkworm pupa and red ginseng, and all of them sequentially extracted and mixed.
본 발명의 구체예에서, 본 발명의 조성물은 항산화용 식품, 화장료, 약학적 조성물 또는 의약외품 조성물일 수 있다.In an embodiment of the present invention, the composition of the present invention may be an antioxidant food, cosmetic, pharmaceutical composition or quasi-drug composition.
본 발명의 조성물이 식품 조성물로 이용되는 경우, 본 발명의 식품 조성물은 질병의 예방 및 개선, 생체방어, 면역, 병후의 회복, 노화 억제 등 생체조절 기능을 가지는 식품을 말하는 것으로, 장기적으로 복용하였을 때 인체에 무해해야 하고, 이는 “건강기능식품” 을 포함하는 개념이다. When the composition of the present invention is used as a food composition, the food composition of the present invention refers to a food having bioregulatory functions such as prevention and improvement of disease, biodefense, immunity, recovery from disease, and suppression of aging. It should be harmless to the human body, and this is a concept that includes “health functional food”.
상기 식품의 종류에는 특별한 제한은 없다. 상기 물질을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 수프, 음료수, 차, 드링크제, 알코올 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the type of the food. Examples of foods to which the above substances can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages, vitamin complexes, and the like, and includes all health foods in the ordinary sense.
본 발명의 실시예에서, 본 발명의 식품 조성물은 식품 첨가물일 수 있다. 상기 식품 첨가물은 상기 누에 번데기 및 홍삼추출물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 혼합양은 사용 목적(예방, 건강 또는 치료적 처치)에 따라 적합하게 결정될 수 있다. 일반적으로, 식품 또는 음료의 제조 시에 본 발명의 누에 번데기 및 홍삼추출물은 원료에 대하여 15중량% 이하, 바람직하게는 10 중량% 이하의 양으로 첨가된다. 그러나, 건강 및 위생을 목적으로 하거나 또는 건강 조절을 목적으로 하는 장기간의 섭취의 경우에는 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로도 사용될 수 있다.In an embodiment of the present invention, the food composition of the present invention may be a food additive. The food additive may be added to the silkworm pupa and red ginseng extract as it is, or may be used with other foods or food ingredients, and may be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined according to the purpose of use (prevention, health or therapeutic treatment). In general, the silkworm pupae and red ginseng extract of the present invention are added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw material when preparing food or beverage. However, in the case of long-term intake for health and hygiene or health control, it may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.
본 발명의 실시예에서, 본 발명의 식품 조성물은 건강음료 조성물일 수 있다. 상기 건강음료 조성물은 누에 번데기 및 홍삼추출물 외에 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 포함할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토오스, 수크로오스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ml 당 일반적으로 약 0.01 내지 10 g, 바람직하게는 약 0.01 내지 0.1 g 이다.In an embodiment of the present invention, the food composition of the present invention may be a health drink composition. The health drink composition may include various flavoring agents or natural carbohydrates as additional ingredients, such as a conventional beverage, in addition to silkworm pupa and red ginseng extract. As the above-mentioned natural carbohydrates, monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and natural sweeteners such as dextrin and cyclodextrin, synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 10 g, preferably about 0.01 to 0.1 g per 100 ml of the composition of the present invention.
상기 외에 본 발명의 조성물은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산 음료에 사용되는 탄산화제 등을 포함할 수 있다. 그 밖에 본 발명의 조성물은 천연 과일주스, 과일주스 음료 및 야채 음료의 제조를 위한 과육을 포함할 수 있다. 이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하진 않지만 본 발명의 조성물 100 중량부 당 0.01 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the above, the composition of the present invention includes various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, Carbonating agents used in carbonated beverages, etc. may be included. In addition, the composition of the present invention may include fruit for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. The proportion of these additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
본 발명의 조성물이 화장료 조성물로 이용되는 경우, 본 발명의 화장료 조성물은 상기 유효성분 이외에 화장료 조성물에 통상적으로 사용되는 항산화제, 안정화제, 용해화제, 비타민, 안료, 향료 등과 같은 통상적인 보조제 및 담체가 더 포함될 수 있다. 예를 들어, 상기 화장료 조성물에는 글리세린, 부틸렌 글라이콜, 폴리옥시에칠렌 경화피마자유, 토코페릴 아세테이트, 시트릭산, 판테놀, 스쿠알란, 소듐 시트레이트, 알란토인 등의 보조성분이 추가로 더 포함될 수 있다.When the composition of the present invention is used as a cosmetic composition, the cosmetic composition of the present invention includes, in addition to the above active ingredients, conventional adjuvants and carriers such as antioxidants, stabilizers, solubilizers, vitamins, pigments, fragrances, etc. that are commonly used in cosmetic compositions. may be further included. For example, the cosmetic composition may further include auxiliary components such as glycerin, butylene glycol, polyoxyethylene hydrogenated castor oil, tocopheryl acetate, citric acid, panthenol, squalane, sodium citrate, and allantoin. .
본 발명의 화장료 조성물은 기본적으로 피부에 도포되는 것이므로, 당업계의 화장료 조성물을 참조하여 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있다. 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 유연 화장수, 영양 화장수, 영양크림, 마사지크림, 에센스, 아이크림, 클렌징크림, 클렌징폼, 클렌징워터, 마스크팩, 스프레이 또는 파우더의 제형으로 제조될 수 있다.Since the cosmetic composition of the present invention is basically applied to the skin, it may be prepared in any formulation conventionally prepared with reference to a cosmetic composition in the art. For example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays, etc. The present invention is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a mask pack, a spray, or a powder.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로 동물성유, 식물성유, 왁스, 파라핀, 전분, 트라칸트, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크, 산화아연 등이 포함될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. may be included as carrier components. have.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트, 폴리아미드 파우더 등이 포함될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄, 디메틸 에테르 등의 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, etc. may be included as carrier components, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane and dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로 용매, 용해화제, 유탁화제 등이 포함될 수 있고, 구체적으로 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜, 소르비탄의 지방산 에스테르 등이 포함될 수 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer, emulsifier, etc. may be included as carrier components, and specifically, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene Glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, and the like may be included.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로 물, 에탄올, 프로필렌글리콜 등의 액상 희석제; 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르, 폴리옥시에틸렌 소르비탄 에스테르 등의 현탁제; 미소결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가, 트라칸트 등이 포함될 수 있다.When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol, propylene glycol; suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester; Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, tracanth, and the like may be included.
본 발명의 제형이 계면-활성제 함유 클린징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성유, 라놀린유도체, 에톡실화 글리세롤 지방산 에스테르 등이 포함될 수 있다.When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, ethoxylated glycerol fatty acid esters, and the like may be included.
상기 누에 번데기 및 홍삼추출물은 화장료 조성물 총 중량에 대하여 0.01 내지 95중량%로 포함되는 것이 바람직하고, 더욱 바람직하게는 1 내지 80중량%로 포함되는 것이다. 그 함량이 0.01중량% 미만일 경우에는 항산화 효과가 미미할 수 있으며, 95중량%를 초과할 경우에는 사용량 대비 효과 상승률이 낮아 비경제적일 수 있다.The silkworm pupae and red ginseng extract are preferably included in an amount of 0.01 to 95% by weight, more preferably 1 to 80% by weight, based on the total weight of the cosmetic composition. If the content is less than 0.01% by weight, the antioxidant effect may be insignificant, and if it exceeds 95% by weight, the increase rate of the effect relative to the amount used is low, which may be uneconomical.
본 발명의 조성물이 약학적 조성물로 이용되는 경우, 본 발명의 약학적 조성물은 통상의 방법에 따라 다양한 형태로 제형화하여 사용될 수 있다. 예컨대, 산제, 과립제, 정제, 캡슐제, 현탁액, 에멀젼, 시럽 등의 경구형 제형으로 제형화할 수 있고, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다.When the composition of the present invention is used as a pharmaceutical composition, the pharmaceutical composition of the present invention may be formulated and used in various forms according to a conventional method. For example, it may be formulated in oral dosage forms such as powders, granules, tablets, capsules, suspensions, emulsions, and syrups, and may be formulated in the form of external preparations, suppositories, and sterile injection solutions.
본 발명의 항산화용 약학적 조성물은 이에 제한되는 것은 아니나, 염증, 빈혈, 심근경색, 뇌성마비, 류머티즘성 관절염, 파킨슨병, 자가면역질환, 심장질환, 암, 당뇨병, 뇌혈관 질환, 동맥경화증 및 이들의 합병증 중에서 선택되는 산화적 스트레스에 의한 질환에 대한 예방 또는 치료 용도일 수 있다. The antioxidant pharmaceutical composition of the present invention is not limited thereto, but inflammation, anemia, myocardial infarction, cerebral palsy, rheumatoid arthritis, Parkinson's disease, autoimmune disease, heart disease, cancer, diabetes, cerebrovascular disease, arteriosclerosis and It may be used for prevention or treatment of diseases caused by oxidative stress selected from their complications.
본 발명의 약학적 조성물은 투여를 위해서 상기 유효성분 이외에 추가로 약학적으로 허용 가능한 담체를 1종 이상 포함하여 제조할 수 있다. 본 발명의 약학적 조성물에 포함되는 약학적으로 허용되는 담체는 제제시에 통상적으로 이용되는 것으로서, 락토오스, 덱스트로스, 수크로스, 솔비톨, 만니톨, 전분, 아카시아 고무, 인산 칼슘, 알기네이트, 젤라틴, 규산 칼슘, 미세결정성 셀룰로스, 폴리비닐피롤리돈, 셀룰로스, 물, 시럽, 메틸 셀룰로스, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 활석, 스테아르산 마그네슘 및 미네랄 오일 등을 포함하나, 이에 한정되는 것은 아니다. 본 발명의 약학적 조성물은 상기 성분들 이외에 윤활제, 습윤제, 감미제, 향미제, 유화제, 현탁제, 보존제 등을 추가로 포함할 수 있다. The pharmaceutical composition of the present invention may be prepared by including one or more pharmaceutically acceptable carriers in addition to the active ingredients for administration. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are commonly used in formulation, and include lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water, syrup, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil, and the like. it's not going to be The pharmaceutical composition of the present invention may further include a lubricant, a wetting agent, a sweetening agent, a flavoring agent, an emulsifying agent, a suspending agent, a preservative, and the like, in addition to the above components.
본 발명의 약학적 조성물의 투여량은 상기 약학적 조성물의 제제화 방법, 투여 방식, 투여 시간 및/또는 투여 경로 등에 의해 다양해질 수 있으며, 상기 약학적 조성물의 투여로 달성하고자 하는 반응의 종류와 정도, 투여 대상이 되는 개체의 종류, 연령, 체중, 일반적인 건강 상태, 질병의 증세나 정도, 성별, 식이, 배설, 해당 개체에 동시 또는 이시에 함께 사용되는 약물 기타 조성물의 성분 등을 비롯한 여러 인자 및 의약 분야에서 잘 알려진 유사 인자에 따라 다양해질 수 있으며, 당해 기술 분야에서 통상의 지식을 가진 자는 목적하는 치료에 효과적인 투여량을 용이하게 결정하고 처방할 수 있다.The dosage of the pharmaceutical composition of the present invention may vary depending on the formulation method, administration method, administration time and/or route of administration of the pharmaceutical composition, and the type and degree of response to be achieved by administration of the pharmaceutical composition. , various factors including the type of subject to be administered, age, weight, general health, symptoms or severity of disease, sex, diet, excretion, components of drugs or other compositions used simultaneously or at the same time in the subject; It may vary depending on similar factors well known in the medical field, and a person skilled in the art can easily determine and prescribe an effective dosage for a desired treatment.
본 발명의 약학적 조성물의 투여량은 예를 들어, 1일 1 mg/kg 내지 1,000 mg/kg일 수 있으나, 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다.The dosage of the pharmaceutical composition of the present invention may be, for example, 1 mg/kg to 1,000 mg/kg per day, but the dosage is not intended to limit the scope of the present invention in any way.
본 발명의 약학적 조성물의 투여 경로 및 투여 방식은 각각 독립적일 수 있으며, 그 방식에 있어 특별히 제한되지 아니하며, 목적하는 해당 부위에 상기 약학적 조성물이 도달할 수 있는 한 임의의 투여 경로 및 투여 방식에 따를 수 있다. The route and mode of administration of the pharmaceutical composition of the present invention may be each independent, and the method is not particularly limited, and any route and mode of administration as long as the pharmaceutical composition can reach the desired site. can follow
상기 약학적 조성물은 경구 투여 또는 비경구 투여 방식으로 투여할 수 있다. 상기 비경구 투여 방식으로는 예를 들어 정맥 내 투여, 복강 내 투여, 근육 내 투여, 경피 투여 또는 피하 투여 등이 포함되며, 상기 약학적 조성물을 질환 부위에 도포하거나 분무, 흡입하는 방법 또한 이용할 수 있으나 이에 제한되지 않는다.The pharmaceutical composition may be administered by oral administration or parenteral administration. The parenteral administration method includes, for example, intravenous administration, intraperitoneal administration, intramuscular administration, transdermal administration, or subcutaneous administration, and methods of applying, spraying, or inhaling the pharmaceutical composition to the diseased site may also be used. However, the present invention is not limited thereto.
또한 본 발명의 누에 번데기 추출물 및 홍삼 추출물은 항산화 목적의 의약외품 조성물일 수 있고, 용어 '의약외품'이란 약사법에 따라 의약품의 용도로 사용되는 물품을 제외한 것으로서, 질병을 치료하거나 예방하기 위해 쓰는 의약품보다 인체에 대한 작용이 경미한 물품에 대해 보건복지부가 따로 정한 분류 기준에 의한 약품을 의미한다. 일 구체에서 상기 의약외품은 항산화 목적의 의약외품일 수 있다.In addition, the silkworm pupa extract and red ginseng extract of the present invention may be a quasi-drug composition for the purpose of antioxidant, and the term 'quasi-drug' excludes articles used for medicinal purposes according to the Pharmaceutical Affairs Act, and is better than medicines used to treat or prevent diseases. It refers to drugs according to the classification criteria set separately by the Ministry of Health and Welfare for goods with a slight effect on the skin. In one embodiment, the quasi-drug may be a quasi-drug for antioxidant purposes.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 이들 실시예에 의해 제한되는 것으로 해석되지는 않는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.
실험예Experimental example 1. 누에 단일 추출물의 항산화 효과 확인 1. Confirmation of antioxidant effect of silkworm single extract
누에 산물의 추출 조건 별 항산화 효과를 확인하기 위한 실험을 수행하였다. 시료로 백옥잠, 골든실크 품종의 누에와 누에 번데기 (울진농원) 를 이용하였다. 추출 조건을 달리하기 위하여 상기 시료들을 각각 90℃ 물을 추출 용매로 하여 24, 48, 72 시간, 30% (v/v) 에탄올을 용매로 하여 24, 48, 72 시간 추출하였다. 각 추출물들의 항산화 효과는 폴리페놀 함량 측정, ABTS 라디칼 제거능, DPPH(1,1-diphenyl-2-picryl hydrazyl) 라디칼 제거능 측정을 통해 확인하였다. An experiment was performed to confirm the antioxidant effect of silkworm products for each extraction condition. As samples, silkworms and pupae (Uljin Farm) of Baekjam, Golden Silk varieties were used. In order to change the extraction conditions, the samples were extracted for 24, 48, 72 hours and 30% (v/v) ethanol for 24, 48, 72 hours using 90° C. water as an extraction solvent, respectively. The antioxidant effect of each extract was confirmed by measuring polyphenol content, ABTS radical scavenging ability, and DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging ability.
폴리페놀 화합물의 함량 비교는 Folin-Ciocalteu phenol reagent를 이용하여 측정하였다. 각 추출물(10 μL)에 2% Na2CO3 용액(200 μL)을 가하고 3분간 방치한 후, 50% Folin-Ciocalteu's reagent (10 μL)을 첨가하여 청색으로 발색되는 것을 750nm에서 측정하였다. 정량값은 0.1% gallic acid를 표준물질로 사용하여 gallic acid equivalent (GAE)값으로 표기하였다. The content comparison of polyphenol compounds was measured using Folin-Ciocalteu phenol reagent. A 2% Na 2 CO 3 solution (200 μL) was added to each extract (10 μL) and left for 3 minutes, and then 50% Folin-Ciocalteu's reagent (10 μL) was added, and the blue color was measured at 750 nm. Quantitative values were expressed as gallic acid equivalent (GAE) values using 0.1% gallic acid as a standard material.
DPPH radical 소거 활성은 trolox 표준곡선을 이용하여 trolox equivalent (TE) 값으로 표기하였으며, 각 추출물 시료 (10 μL)에 DPPH 용액(200 μL) 을 가하여 실온에 30분 동안 방치한 후 흡광도를 520nm에서 측정하여 확인하였다.The DPPH radical scavenging activity was expressed as a trolox equivalent (TE) value using a trolox standard curve. After adding a DPPH solution (200 μL) to each extract sample (10 μL) and leaving it at room temperature for 30 minutes, the absorbance was measured at 520 nm. to confirm.
ABTS radical을 이용한 항산화력 측정은 ABTS+양이온 탈색 분석 방법(Re et al., 1999)에 의하여 시행하였다. 7.4 mM ABTS(Sigma Co.)와 2.6 mM 과황산칼륨(potassium persulfate)(Sigma Co.)를 최종농도로 혼합하여 실온인 암소에서 24시간 동안 방치하여 ABTS radical을 형성시킨 후 734 nm에서 흡광도 값이 1.0이 되게 PBS(pH7.4)로 희석하였다. 희석된 용액 200 μL에 추출물 시료 10 μL를 가하여 정확히 10분 동안 방치한 후 흡광도를 734 nm 측정하였다. 최종 결과값은 trolox 표준곡선을 이용하여 TE 값으로 환산하였다. 측정된 결과는 각 도 1 내지 도 3에 나타내었다.Antioxidative activity using ABTS radical was measured by ABTS+cation decolorization analysis method (Re et al., 1999). 7.4 mM ABTS (Sigma Co.) and 2.6 mM potassium persulfate (Sigma Co.) were mixed to a final concentration and left in the dark at room temperature for 24 hours to form ABTS radicals. It was diluted with PBS (pH 7.4) to 1.0. After adding 10 µL of the extract sample to 200 µL of the diluted solution and leaving it for exactly 10 minutes, the absorbance was measured at 734 nm. The final result value was converted to TE value using the trolox standard curve. The measured results are shown in FIGS. 1 to 3 , respectively.
도 1 내지 도 3에 나타낸 바와 같이, 누에 산물 추출물의 항산화 효과를 비교 실험한 결과, 누에 번데기를 추출 대상으로 하고, 이를 30% 에탄올로 추출한 실험군에서 다른 누에 품종의 추출물과 누에 번데기 수 추출물 대비 현저하게 우수한 폴리페놀 함량을 나타내며, ABTS 라디칼, DPPH 라디칼 소거능 역시 현저하게 우수함을 확인하였다. As shown in FIGS. 1 to 3 , as a result of a comparative experiment on the antioxidant effect of the silkworm product extract, the silkworm pupa was extracted, and in the experimental group extracted with 30% ethanol, it was significantly better than the extracts of other silkworm varieties and the number of silkworm pupa extracts. It was confirmed that the polyphenol content was excellent, and the ABTS radical and DPPH radical scavenging ability were also remarkably excellent.
실험예Experimental example 2. 홍삼 단일 추출물의 항산화 효과 확인 2. Confirmation of antioxidant effect of single extract of red ginseng
홍삼 단일 추출물의 추출 조건 별 항산화 효과를 확인하기 위한 실험을 수행하였다. 추출 조건을 달리하기 위하여 홍삼 시료들을 상압 및 상온에서 추출하되, 추출 용매를 100% (v/v), 50% (v/v) 에탄올 및 물로 달리하여 24 시간 추출하였다. 실험예 1과 동일한 방법을 통해 홍삼 단일 추출물의 추출 조건 별 폴리페놀 함량 측정, ABTS 라디칼 제거능, DPPH(1,1-diphenyl-2-picryl hydrazyl) 라디칼 제거능 측정을 수행하였으며, 그 결과를 도 4 내지 도 6에 나타내었다.An experiment was performed to confirm the antioxidant effect of a single extract of red ginseng for each extraction condition. In order to change the extraction conditions, red ginseng samples were extracted at normal pressure and room temperature, and the extraction solvents were 100% (v/v), 50% (v/v) ethanol and water, and extracted for 24 hours. In the same manner as in Experimental Example 1, polyphenol content measurement, ABTS radical removal ability, and DPPH (1,1-diphenyl-2-picryl hydrazyl) radical removal ability of a single extract of red ginseng for each extraction condition were measured, and the results are shown in FIGS. 4 to 6 shows.
도 4 내지 도 6에 나타낸 바와 같이, 홍삼 추출물의 항산화 효과를 비교 실험한 결과, 50% (v/v) 에탄올을 용매로 추출한 홍삼 추출물이 수 추출물 또는 100%(v/v) 에탄올로 추출한 실험군 대비 폴리페놀 함량 및 ABTS 라디칼 소거능이 우수하며, DPPH 라디칼 소거능이 100%(v/v) 에탄올 추출물 대비 현저하게 우수함을 확인하였다. 4 to 6, as a result of a comparative experiment on the antioxidant effect of the red ginseng extract, the experimental group in which the red ginseng extract obtained by extracting 50% (v/v) ethanol as a solvent was extracted with water extract or 100% (v/v) ethanol. It was confirmed that the polyphenol content and ABTS radical scavenging ability were excellent, and the DPPH radical scavenging ability was significantly superior to that of 100% (v/v) ethanol extract.
실시예Example 1. 누에 번데기 및 홍삼 혼합 추출물의 항산화 효과 1. Antioxidant effect of silkworm pupa and red ginseng mixed extract
상기 실험예 1 및 2를 통해 누에 번데기 30%(v/v) 에탄올 추출물 및 홍삼 50% (v/v) 에탄올 추출물이 다른 추출 조건에 의한 추출물 대비 우수한 항산화 효과를 나타냄을 확인하였으므로, 이들을 혼합한 혼합 추출물의 항산화 효과를 확인하였다. 누에 번데기 추출물 및 홍삼 추출물은 하기와 같은 비율로 혼합 비율을 조정하여 제조하였다. 누에 번데기 30%(v/v) 에탄올 추출물 및 홍삼 50% (v/v) 에탄올 추출물을 각각 단일 추출물 비교예로 설정하였다. As it was confirmed that the silkworm pupae 30% (v/v) ethanol extract and the
(25:75)(25:75)
(50:50)(50:50)
(75:25)(75:25)
(25:75)(25:75)
(50:50)(50:50)
(75:25)(75:25)
실험예 1에 기재된 방법과 동일한 방법으로, 누에 번데기 단일 추출물, 홍삼 단일 추출물, 표 1에 따른 누에 번데기 및 홍삼 혼합 추출물의 DPPH 라디칼 제거능 측정을 수행하였으며, 그 결과를 도 7에 나타내었다.In the same manner as described in Experimental Example 1, the DPPH radical scavenging ability of silkworm pupa single extract, red ginseng single extract, and silkworm pupa and red ginseng mixed extract according to Table 1 was measured, and the results are shown in FIG. 7 .
도 7에 나타낸 바와 같이, 누에 번데기 및 홍삼의 혼합추출물은 누에 번데기 단일 추출물, 홍삼 단일 추출물 대비 DPPH 라디칼 소거능이 우수하였으며, 특히 이를 50:50, 75:25 로 혼합한 혼합 실시예 2 및 3에서 누에 번데기 단일 추출물 대비 2 배 이상의 효과를 확인하였다. As shown in Figure 7, the mixed extract of silkworm pupa and red ginseng had superior DPPH radical scavenging ability compared to silkworm pupa single extract and red ginseng single extract. It was confirmed that the effect was more than twice that of the silkworm pupa single extract.
또한 누에 번데기 단일 추출물, 홍삼 단일 추출물, 표 2에 따른 누에 번데기 및 홍삼 혼합 추출물의 세포내 활성 산소종 (reactive oxygen species, ROS) 소거능을 확인하였다. 구체적으로 세포 내에서 생성된 ROS의 양을 측정하기 위해서 2', 7'-dichloro-dihydro-fluorescein diacetate (DCFH-DA)를 이용한 Lee 등(2017)의 방법을 변형하여 실험하였다. 배양 중인 세포를 black 96 well plate에 5×104의 농도로 각 well에 분주하고 12시간 후 단일 추출물 및 혼합 추출물을 serum-free DMEM에 녹여 12시간 동안 전처리하였다. 이후 각 well에 tert-Butyl hydroperoxide (tBHP)를 최종농도가 1 mM이 되도록 첨가하였다. 1시간 후 배지를 제거하고 DCFH-DA 25 μM을 1시간 처리하였다. 최종 세포내 ROS 농도는 485/530 nm 에서 형광값으로 측정하였으며, 그 결과를 도 8에 나타내었다. 대조군으로는 tBHP 처리군을 이용하였다. In addition, the intracellular reactive oxygen species (ROS) scavenging ability of silkworm pupa single extract, red ginseng single extract, and silkworm pupa and red ginseng mixed extract according to Table 2 was confirmed. Specifically, in order to measure the amount of ROS generated in cells, the method of Lee et al. (2017) using 2',7'-dichloro-dihydro-fluorescein diacetate (DCFH-DA) was modified and tested. Cells in culture were dispensed into each well at a concentration of 5×10 4 in a black 96 well plate, and after 12 hours, single and mixed extracts were dissolved in serum-free DMEM and pretreated for 12 hours. Thereafter, tert-Butyl hydroperoxide (tBHP) was added to each well to a final concentration of 1 mM. After 1 hour, the medium was removed and DCFH-DA 25 μM was treated for 1 hour. The final intracellular ROS concentration was measured as a fluorescence value at 485/530 nm, and the results are shown in FIG. 8 . As a control group, the tBHP-treated group was used.
도 8에 나타낸 바와 같이, 미처리 대조군 대비 tBHP 첨가군에서는 세포내 활성 산소종이 현저하게 증가하였고, 누에 번데기 및 홍삼 혼합 추출물 처리 실험군에서는 현저하게 우수한 ROS 제거 효과를 확인하였다. 특히 혼합 실시예 3에서는 누에 번데기 단일 추출물 대비 2.2 배 ROS 제거 효과를 확인하여 단일 추출물 대비 누에 번데기 추출물 및 홍삼 추출물을 포함하는 혼합 추출물이 현저히 우수한 효과를 나타낼 수 있음을 확인하였다. As shown in FIG. 8 , intracellular reactive oxygen species was significantly increased in the tBHP-added group compared to the untreated control group, and a remarkably excellent ROS removal effect was confirmed in the experimental group treated with silkworm pupa and red ginseng mixed extract. In particular, in Mixed Example 3, compared to the single extract of silkworm pupa, a 2.2-fold ROS removal effect was confirmed, and it was confirmed that the mixed extract containing the silkworm pupa extract and red ginseng extract could exhibit a remarkably excellent effect compared to the single extract.
이하, 제제예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 제제예는 오로지 본 발명을 예시하기 위한 것으로서, 본 발명의 범위가 제제예에 의해 제한되는 것으로 해석되지 않는다.Hereinafter, the present invention will be described in more detail through formulation examples. The formulation examples are only for illustrating the present invention, and the scope of the present invention is not to be construed as being limited by the formulation examples.
제제예Formulation example 1. 항산화용 약학적 조성물의 제조 1. Preparation of pharmaceutical composition for antioxidant
1-1. 1-1. 산제의powdery 제조 Produce
누에 번데기 추출물 및 홍삼 추출물 20 mgSilkworm pupa extract and
유당 100 mg
탈크 10 mg
상기의 성분들을 혼합하고 기밀포에 충진하여 산제를 제조한다.The above ingredients are mixed and filled in an airtight bag to prepare a powder.
1-2. 정제의 제조1-2. manufacture of tablets
누에 번데기 추출물 및 홍삼 추출물 10 mg10 mg of silkworm pupa extract and red ginseng extract
옥수수전분 100 mg100 mg cornstarch
유당 100 mg
스테아린산 마그네슘 2 mg2 mg magnesium stearate
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.After mixing the above ingredients, tablets are prepared by tableting according to a conventional manufacturing method of tablets.
1-3. 캡슐제의 제조1-3. manufacture of capsules
누에 번데기 추출물 및 홍삼 추출물 10 mg10 mg of silkworm pupa extract and red ginseng extract
결정성 셀룰로오스 3 mg3 mg of crystalline cellulose
락토오스 14.8 mgLactose 14.8 mg
마그네슘 스테아레이트 0.2 mg0.2 mg magnesium stearate
통상의 캡슐제 제조방법에 따라 상기의 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조한다.According to a conventional capsule preparation method, the above ingredients are mixed and filled in a gelatin capsule to prepare a capsule.
1-4. 주사제의 제조1-4. manufacture of injections
누에 번데기 추출물 및 홍삼추출물 10 mgSilkworm pupa extract and
만니톨 180 mgmannitol 180 mg
주사용 멸균 증류수 2974 mg2974 mg of sterile distilled water for injection
Na2HPO42H2O 26 mgNa 2 HPO 4 2H 2 O 26 mg
통상의 주사제의 제조방법에 따라 1 앰플당 (2 ml) 상기의 성분 함량으로 제조한다.According to a conventional method for preparing injections, the content of the above ingredients per 1 ampoule (2 ml) is prepared.
1-5. 1-5. 액제의liquid 제조 Produce
누에 번데기 추출물 및 홍삼추출물 20 mgSilkworm pupa extract and
이성화당 10 g10 g isomerized sugar
만니톨 5 g5 g of mannitol
정제수 적량Purified water appropriate amount
통상의 액제의 제조방법에 따라 정제수에 각각의 성분을 가하여 용해시키고 레몬향을 적량 가한 다음 상기의 성분을 혼합한 다음 정제수를 가하여 전체를 정제수를 가하여 전체 100ml로 조절한 후 갈색병에 충진하여 멸균시켜 액제를 제조한다.According to a conventional liquid preparation method, each component is added to purified water to dissolve, an appropriate amount of lemon flavor is added, the above components are mixed, purified water is added, the whole is adjusted to 100ml by adding purified water, and then filled in a brown bottle and sterilized to prepare a liquid.
제제예Formulation example 2. 항산화용 2. For antioxidant 화장료cosmetics 조성물의 제조 Preparation of the composition
2-1. 유연화장수(스킨로션)의 제조2-1. Manufacture of softening lotion (skin lotion)
누에 번데기 추출물 및 홍삼추출물 0.5%Silkworm pupa extract and red ginseng extract 0.5%
베타-1,3-글루칸 1.0%Beta-1,3-glucan 1.0%
부틸렌글리콜 2.0%Butylene glycol 2.0%
프로필렌글리콜 2.0%Propylene glycol 2.0%
카르복시비닐폴리머 0.1%Carboxyvinyl Polymer 0.1%
피이지-12 노닐페닐에테르 0.2%PEG-12 Nonylphenyl Ether 0.2%
폴리솔베이트 80 0.4%Polysorbate 80 0.4%
에탄올 10.0%Ethanol 10.0%
트리에탄올아민 0.1%Triethanolamine 0.1%
방부제, 색소, 향료 적량Preservatives, colors and fragrances
정제수 to 100%Purified water to 100%
2-2. 영양화장수(2-2. Nutrient lotion ( 밀크로션milk lotion )의 제조) manufacturing
누에 번데기 추출물 및 홍삼추출물 0.5 %Silkworm pupa extract and red ginseng extract 0.5%
베타-1,3-글루칸 1.0 %Beta-1,3-glucan 1.0%
밀납 4.0 %beeswax 4.0%
폴리솔베이트 60 1.5 %Polysorbate 60 1.5%
솔비탄세스퀴올레이트 1.5 %Sorbitan sesquioleate 1.5%
유동파라핀 0.5 %Liquid paraffin 0.5%
카프릴릭/카프릭트리글리세라이드 5.0 %Caprylic/Capric Triglycerides 5.0 %
글리세린 3.0 %Glycerin 3.0%
부틸렌글리콜 3.0 %Butylene Glycol 3.0 %
프로필렌글리콜 3.0 %Propylene glycol 3.0 %
카르복시비닐 폴리머 0.1 %Carboxyvinyl Polymer 0.1%
트리에탄올아민 0.2 %Triethanolamine 0.2%
방부제, 색소, 향료 적량Preservatives, colors and fragrances
정제수 to 100 %Purified water to 100 %
2-3. 영양크림의 제조2-3. production of nourishing cream
누에 번데기 추출물 및 홍삼추출물 1.0 %Silkworm pupa extract and red ginseng extract 1.0%
베타-1,3-글루칸 5.0 %Beta-1,3-glucan 5.0%
밀날 10.0 %wheat blade 10.0 %
폴리솔베이트 60 1.5 %Polysorbate 60 1.5%
피이지 60 경화피마자유 2.0 %Sebum 60 hydrogenated castor oil 2.0%
솔비탄세스퀴올레이트 0.5 % Sorbitan sesquioleate 0.5%
유동파라핀 10.0 %Liquid paraffin 10.0 %
스쿠알란 5.0 %Squalane 5.0%
카프릴릭/카프릭트리글리세라이드 5.0 %Caprylic/Capric Triglycerides 5.0 %
글리세린 5.0 %Glycerin 5.0%
부틸렌글리콜 3.0 %Butylene Glycol 3.0 %
프로필렌글리콜 3.0 %Propylene glycol 3.0 %
트리에탄올아민 0.2 %Triethanolamine 0.2%
방부제, 색소, 향료 적량Preservatives, colors and fragrances
정제수 to 100%Purified water to 100%
이상, 본 발명내용의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서, 이러한 구체적인 기술은 단지 바람직한 실시양태일 뿐이며, 이에 의해 본 발명의 범위가 제한되는 것이 아닌 점은 명백할 것이다. 따라서 본 발명의 실질적인 범위는 첨부된 청구항들과 그것들의 등가물에 의해 정의된다고 할 것이다. Above, a specific part of the present invention has been described in detail, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.
Claims (9)
25 to 35% (v/v) ethanol-extracted silkworm pupa extract and 50% (v/v) ethanol-extracted red ginseng extract, wherein the silkworm pupa extract and red ginseng extract are mixed in a 1:1 to 3:1 volume ratio A food composition for antioxidants, characterized in that.
According to claim 1, wherein the silkworm pupa extract and red ginseng extract is 1:1 or 3:1 volume ratio, characterized in that mixed, antioxidant food composition.
The method of claim 1, wherein the composition has a radical scavenging activity of ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) or DPPH (2,2-diphenyl-1-picrylhydrazyl) A food composition for antioxidants.
According to claim 1, wherein the composition has a reactive oxygen species (reactive oxygen species) scavenging activity, characterized in that, antioxidant food composition.
25 to 35% (v/v) ethanol-extracted silkworm pupa extract and 50% (v/v) ethanol-extracted red ginseng extract, wherein the silkworm pupa extract and red ginseng extract are mixed in a 1:1 to 3:1 volume ratio A cosmetic composition for antioxidants, characterized in that it has been
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| KR101703527B1 (en) | 2015-12-07 | 2017-02-08 | 주식회사 유니솔브 | A composition for antioxidating, whitening and improving wrinkle comprising extracts of bombycis faeces |
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| KR20060014687A (en) * | 2004-08-11 | 2006-02-16 | 최진호 | Functional erectile accelerator based on red ginseng extract, its preparation method and strengthening supplement |
| KR100573466B1 (en) * | 2004-09-23 | 2006-05-02 | 대한민국(관리부서:농촌진흥청) | Extraction, Separation and Purification of Oil from Silkworm Pupa |
| KR101358507B1 (en) * | 2011-11-22 | 2014-02-06 | 주식회사 에이세프 | Composition for antiinflammatory, antibacterial and removing stench comprising red ginseng extracts and extracts derived from natural materials |
| KR101420781B1 (en) * | 2012-12-07 | 2014-07-21 | 주식회사 대화약품 | Method for manufacturing pill product using silkworm |
| KR20170004626A (en) * | 2015-07-03 | 2017-01-11 | 김혜정 | A cosmetic composition using silkworm-derived extracts |
| KR101863694B1 (en) * | 2015-08-04 | 2018-06-01 | 주식회사 한국인삼공사 | Cosmetic Composion for Anti-oxidation Containing Purified Extract of Red Ginseng Ethanol Extract |
| KR101906143B1 (en) * | 2016-11-25 | 2018-12-06 | 주식회사코리아코스팩 | A cosmetic composition for the extract of golden cocoon with improved anti-aging activity by far-infrared method |
| KR20190021614A (en) * | 2017-08-23 | 2019-03-06 | 태웅식품 주식회사 | Composition for antioxidant, antiinflammatory and inflammatory neurodegenerative diseases comprising perilla frutescens britton extract |
| KR20190069740A (en) * | 2017-12-12 | 2019-06-20 | 김종현 | Red ginseng drink containing grub extracts |
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