KR102430622B1 - Low irritant detergent composition - Google Patents

Abstract

The present invention relates to a hypoallergenic detergent composition using a betaine-based amphoteric surfactant and two or more specific nonionic surfactants in essential combination, preferably cocamidopropylbetaine as the betaine-based amphoteric surfactant. (Cocamidopropyl Betaine) may be used, and Cocamide MEA and Cocamide Methyl MEA may be used as the nonionic surfactant. In addition, skin hypoallergenicity in the present invention is characterized in that the increase in the size of micelles of the detergent composition prevents the penetration of the surfactant into the skin, and is characterized in that it appears by reducing protein denaturation and lipid elution of the skin.

Description

Low irritant detergent composition

The present invention relates to a human body cleansing composition with low skin irritation by using a betaine-based amphoteric surfactant in combination with two or more specific nonionic surfactants.

The human body cleanser is intended to remove contamination from the body by washing away sweat and sebum secreted by human metabolic activity, and contains a surfactant, which is a cleaning component, as a main component. Conventionally, sulfate-based anionic surfactants having excellent cleaning and foaming power have been widely used in detergents, but as problems of skin irritation and harm to the human body emerged, they were gradually reluctant to use them. Recently, instead of sulfate-based surfactants such as sodium lauryl ether sulfate (SLES), various methods have been sought to find a composition of a surfactant with low irritation while maintaining cleaning power.

For example, the existing sulfate- and sulfonate-based anionic surfactants are used as hypoallergenic anionic surfactants such as sodium cocoyl ethionate, disodium lauryl sulfosuccinate, acyl-N-methyl taurate, and acyl glutamate. Products with reduced skin irritation are being developed by replacing them. However, these surfactants are not very irritating to people with normal skin, but can be felt as a great irritation to people with skin diseases such as atopy or acne.

On the other hand, there have been attempts to make detergents using only a small amount of anionic surfactants to reduce irritation or mainly using nonionic surfactants known to have relatively little skin irritation. Detergents that mainly use nonionic surfactants have a disadvantage in that their performance is very low as a human body cleanser because their foaming power is greatly reduced.

Recently, instead of anionic surfactants, amphoteric surfactants, which are harmful to the human body and have a low degree of skin irritation, have been used in human body cleansers. In particular, betaine-based amphoteric surfactants, including Cocamidopropyl Betaine, are widely used in detergents for hair and skin cleaning. Although it is low, there is still concern about skin irritation.

Therefore, there is a need for a better method for the development of an excellent cleaning agent with less skin irritation while maintaining cleaning power.

1. Republic of Korea Patent Application No. 10-2015-0078130 (published on May 20, 2016) 2. US Patent No. 14503470 (published on April 07, 2016)

Conventional human body cleaners have excellent cleaning power, but have high skin irritation or low human safety. Therefore, it is an object to provide a detergent composition with reduced skin irritation by using a composition and mixing ratio of a surfactant that has a low degree of skin irritation and can be safe for the human body without losing cleaning power.

In order to achieve the above object, the present invention designed a detergent composition formulation with excellent quality and low irritation by using a betaine-based amphoteric surfactant and two or more fatty acid alkanolamide-based nonionic surfactants together.

A preferred component as the betaine-based amphoteric surfactant is cocamidopropyl betaine, and the fatty acid alkanolamide-based nonionic surfactant is Cocamide MEA and Cocamide MEA Methyl MEA) is preferred.

At this time, the Cocamide MEA and Cocamide Methyl MEA may be mixed in a certain ratio and included in the detergent composition according to the present invention.

The cleaning composition according to the present invention exhibits reduced skin irritation while having a cleaning power similar to that of a conventional cleaning composition. In particular, by using a betaine-based amphoteric surfactant and two or more specific nonionic surfactants together, the micelle size of the surfactant increases and skin penetration is reduced, and the effect of reducing protein denaturation and lipid dissolution of the skin is shown, thereby irritating the skin as a whole. will decrease

1 is a diagram illustrating three causes of skin irritation caused by detergents. The cleaning composition according to the present invention is designed to reduce or prevent these causes of skin irritation.

The present invention relates to a cleaning composition with reduced skin irritation while having similar cleaning power to conventional cleaners, and contains a betaine-based amphoteric surfactant and contains a small amount of two or more fatty acid alkanolamide-based nonionic surfactants. By using it, it is possible to exhibit skin hypoallergenic properties without reducing the total content of the surfactant.

The 'cleansing composition' referred to in the present specification can be widely used as a cleaning composition that can be applied to the human body including skin, hair, oral cavity, etc. can

Hereinafter, the present invention will be described in more detail.

The hypoallergenic detergent composition according to the present invention comprises a betaine-based amphoteric surfactant; and at least two of fatty acid alkanolamide-based nonionic surfactants; Including, the betaine-based amphoteric surfactant is preferably cocamidopropyl betaine, and the fatty acid alkanolamide-based nonionic surfactant is cocamide MEA and cocamide methyl M It is preferable that it is E-E (Cocamide Methyl MEA).

Betaine-based amphoteric surfactant

In the present invention, the betaine-based amphoteric surfactant is a naturally-derived component that is relatively safe for the human body and has a lower skin irritation level than anionic surfactant without losing cleaning power. It can be used in place of a surfactant. In particular, it can be used in place of sulfate-based surfactants, which have excellent cleaning power but high skin irritation and low human safety.

The betaine-based amphoteric surfactants include cocamidopropyl betaine, coco-betaine, coco dimethyl carboxymethyl betaine, lauryl dimethyl carboxymethyl betaine. (Lauryl Dimethyl Carboxymethyl Betaine), Lauryl Dimethyl Alphacarboxyethyl Betaine, Cetyl Dimethyl Carboxymethyl Betaine, Lauryl-bis-(2-hydroxyethyl) Carboxymethyl Betaine (Lauryl Bis-(2-hydroxyethyl) Carboxymethyl Betaine), Stearyl Bis-(2-hydroxypropyl) Carboxymethyl Betaine, Oleyldimethyl- Oleyl Dimethyl Gamma-carboxypropyl Betaine, Lauryl Bis-(2-hydroxypropyl)alpha-carboxyethyl Betaine, Soiami Dopropyl Betaine (Soyamidopropyl Betaine), Coco Dimethyl Sulfopropyl Betaine, Stearyl Dimethyl Sulfopropyl Betaine, Cocoamidodimethyl Sulfopropyl Betaine, Lauryl amidodimethylcarboxy Methylbetaine, cetylamidodimethylcarboxymethylbetaine and stearylamidodimethylsulfopropylbetaine, Cocamidopropyl Hydroxysultaine, Coco-Hydroxysultaine, Coco-Sultaine Coco-Sultaine, Erucamidopropyl Hydroxysultaine, Hyde Hydroxysultaines, Lauramidopropyl Hydroxysultaine, Lauryl Hydroxysultaine, Lauryl Sultaine, Methoxycinidopropyl Hydroxysultaine (Methoxycinidopropyl Hydroxysultaine) At least one selected from the group consisting of Hydroxysultaine), Myristamidopropyl Hydroxysultaine, Oleamidopropyl Hydroxysultaine, and Tallowamidopropyl Hydroxysultaine, etc. can be used Preferably, cocamidopropyl betaine or lauryl hydroxy sultaine may be used.

In the detergent composition according to the present invention, the betaine-based amphoteric surfactant may be included in an amount of 10 to 30% by weight, preferably 15 to 30% by weight, based on the total weight of the composition. If the content of the betaine-based amphoteric surfactant is less than 10% by weight, the foaming power and cleaning power are rapidly lowered, and if it is 30% by weight or more, the foaming power is excellent, but the cleaning power is excessive and adversely affects the formulation stability.

nonionic surfactant

It may be difficult to obtain a satisfactory hypoallergenic effect on the skin only by using the betaine-based amphoteric surfactant. Accordingly, the present inventors have discovered that, when two or more specific nonionic surfactants are used together with the betaine-based amphoteric surfactant, a much better skin hypoallergenic effect can be obtained and completed the present invention.

Therefore, the detergent composition according to the present invention includes a nonionic surfactant as an essential component, and the nonionic surfactant is a fatty acid alkanolamides, particularly an alkanolamide of a fatty acid having C8 to C18 carbon atoms, Examples include coconut fatty acid monoethanolamide, monoethanolamide and diethanolamide of lauric acid, monoethanolamide and diethanolamide of myristic acid, and monoethanolamide and diethanolamide of stearic acid. can do. Preferably, the fatty acid alkanolamide-based surfactant may include cocamide MEA and cocamide methyl MEA.

As a high melting point nonionic surfactant, Cocamide MEA is known for its role in raising the foaming power, and Cocamide Methyl MEA is a nonionic surfactant and also has a viscosity control effect as well as a bubble boosting effect. In the present invention, when the Cocamide MEA and Cocamide Methyl MEA are mixed in an appropriate ratio, each bubble boosting effect or viscosity control effect, as well as the synergy of the two components, can maximize the skin hypoallergenic effect. was confirmed.

In the detergent composition according to the present invention, the fatty acid alkanolamide-based nonionic surfactant may be contained in an amount of 0.5% to 5% by weight, preferably 1.5% to 3.5% by weight, based on the total weight of the composition. If the content of the fatty acid alkanolamide-based nonionic surfactant is less than 0.5% by weight, the bubble boosting effect is insignificant, and if it is more than 5% by weight, it adversely affects stability over time.

In the cleaning composition according to the present invention, the content of the cocamide MEA may be contained in an amount of 0.5 to 3% by weight, preferably 0.5 to 1.5% by weight, based on the total weight of the composition. If it is less than 0.5 wt%, the bubble boosting effect is insignificant, and if it is 3 wt% or more, the stability over time of the formulation may be deteriorated.

In the cleaning composition according to the present invention, the content of the cocamide methyl MEA may be contained in an amount of 0.5 to 4% by weight, preferably 1 to 2% by weight, based on the total weight of the composition. If it is less than 0.5% by weight, the bubble boosting effect is insignificant, and if it is more than 4% by weight, the stability over time of the formulation may be deteriorated.

In addition, in the detergent composition according to the present invention, it is most preferable to use the cocamide MEA and cocamide methyl MEA in a ratio of 1.5:2 by weight.

other ingredients

In addition to the surfactant, the detergent composition according to the present invention includes Disodium Cocoamphodiacetate, Sodium Cocoamphoacetate, Alkyl Poly Glucoside, Laureth-4, Disodium Laureth Sulfosuccinate, Sodium Methyl Cocoyl Taurate, Disodium Cocoyl Glutamate, Potassium Cocoyl Glycinate It may further include one or more types from the group consisting of.

The detergent composition according to the present invention may contain a weak acid for pH adjustment. The weak acid is one selected from the group consisting of Citric acid, Tartaric acid, Phosphoric acid, Acetic acid, Maleic acid, Fumaric acid, and combinations thereof. or more, preferably citric acid may be used.

The weak acid may be included in an amount of 0.1 to 0.5% by weight based on the total weight of the cleaning composition. If the content of the weak acid is less than 0.1% by weight, the cleaning power may be reduced, and if it is more than 0.5% by weight, the acidity of the cleaning composition may increase and thus may not be suitable as a cleaning composition.

The cleaning composition according to the present invention may include a moisturizing agent for moisturizing and foaming power of the human body including skin, hair, oral cavity, and the like. The moisturizing agent is sorbitol, glycerin, polyethylene glycol (PEG, polyethylene glycol), propylene glycol, butylene glycol, hyaluronic acid, panthenol, tocopherol. (Tocopherol) and may be at least one selected from a combination thereof, preferably sorbitol (Sorbitol) may be used.

The moisturizing agent may be included in an amount of 5 to 30% by weight based on the total weight of the cleaning composition. If the content of the humectant is less than 5% by weight, it may cause a dry feeling of use, and if it is more than 30% by weight, the ratio of the moisturizing component in the cleaning composition may be excessively increased, so that the feeling of use may not be good.

The cleaning composition according to the present invention may include tetrasodium EDTA as a chelating agent for metal ion sequestration and strengthening the antiseptic power of the product, preferably disodium EDTA. may include.

The chelating agent may be included in an amount of 0.01 to 0.1% by weight based on the total weight of the cleaning composition. If the content of the chelating agent is less than 0.01% by weight, the effect on the preservative power of the product is insignificant, and if it exceeds 0.1% by weight, the foaming power may be reduced.

The detergent composition according to the present invention may contain water, preferably deionized distilled water may be used, and may be used as the balance so that the total weight of the total composition satisfies 100% by weight.

The cleaning composition according to the present invention may further include additives commonly used in the art. For example, it may include one or more additives selected from preservatives, fragrances, viscosity modifiers, conditioning agents, and disinfectants. Such additives may be included, for example, within the range of 0.001 to 10% by weight, depending on the type of each additive based on the total weight of the cleaning composition according to the present invention. In addition, solvents such as alcohols or ketones, herbal extracts, fruit extracts, vitamins and pigments may be optionally further included.

The skin hypoallergenic effect of the detergent composition according to the present invention is the betaine-based amphoteric surfactant such as cocamidopropylbetaine, and the fatty acid alkanolamide-based nonionic surfactant, for example, cocamide MEA and cocamide methyl. By using MEA together, the size of micelles of the detergent composition increases to prevent penetration of the surfactant into the skin, and it is characterized by reducing protein denaturation and lipid dissolution of the skin, which are often caused by detergents containing surfactants. .

The detergent composition according to the present invention may be formulated as one selected from body cleanser, soap, cleansing foam, shampoo, rinse, hair treatment, and combinations thereof, and may be widely used as a human body wash of various formulations.

Hereinafter, the present invention will be described in more detail by way of Examples. However, the embodiments according to the present invention may be modified in various other forms, and the technical scope of the present invention is not limited to the following examples.

[Preparation of Examples and Comparative Examples]

Examples 1-3

The detergent compositions of Examples 1 to 3 were prepared according to the composition and weight shown in Table 1, and the preparation method was as follows (unit: wt%).

(1) After weighing purified water in a beaker, add disodium EDTA and sufficiently stir and dissolve using an Agi Mixer. At this time, the temperature is maintained at 40°C.

(2) After raising the temperature to 60°C, add Cocamide MEA in step (1) to sufficiently dissolve it with an azimuth mixer.

(3) When it is confirmed that dissolved in (2), cocamidopropyl betaine, disodium laureth sulfosuccinate, and disodium cocoamphodiacetate are added to transparently solubilize the dissolved cocamide MEA.

(4) After dispersing by adding sorbitol in (3), citric acid is added to adjust the pH to between 5 and 6.

(5) Add cocamide methyl MEA in (4) and stir and mix to give the formulation a thickening effect and increase hypoallergenicity.

In the manufacturing process, cocamidopropyl betaine was used as Mitaine CA(S) (Miwon Trading Co., Ltd.), but is not limited thereto. In addition, Cocamide MEA used HISOL CME (Miwon Trading Co., Ltd.), and Cocamide Methyl MEA used Micopol CMM (Miwon Trading Co., Ltd.), but is not limited thereto.

Raw material name Example 1 Example 2 Example 3 Surfactants Cocamidopropyl Betaine 26 30 35 Cocamide MEA 1.5 One 0.5 Cocamide Methyl MEA 2 1.5 One Disodium Laureth Sulfosuccinate 15 10 5 Disodium Cocoamphodiacetate 5 10 10 moisturizer Sorbitol 10 10 10 PH regulator Citric acid 0.15 0.15 0.15 chelating agent EDTA-2NA 0.05 0.05 0.05 solvent Purified water to 100 to 100 to 100

Comparative Examples 1 to 6

However, prepared in the same method and sequence as in Examples, a detergent composition was prepared according to the composition shown in Table 2 below (unit: wt%).

Comparative Example 1 had the same composition as that of Example 1, but did not include a nonionic surfactant, and Comparative Examples 2 and 4 had the same composition as Example 1, but used Cocamide MEA as a nonionic surfactant. It was used alone, and Comparative Examples 3 and 5 had the same composition as that of Example 1, but cocamide methyl MEA was used alone as a nonionic surfactant. Comparative Example 6 had the same composition as that of Example 1, but used an alkyl polyglucoside as a nonionic surfactant.

Raw material name Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Surfactants Cocamidopropyl Betaine 26 26 26 26 26 26 Cocamide MEA 0 One 0 3.5 0 0 Cocamide Methyl MEA 0 0 2 0 3.5 0 Disodium Laureth Sulfosuccinate 15 15 15 15 15 15 Disodium Cocoamphodiacetate 5 5 5 5 5 5 Alkyl polyglucoside 0 0 0 0 0 5 moisturizer Sorbitol 10 10 10 10 10 10 PH regulator Citric acid 0.15 0.15 0.15 0.15 0.15 0.15 chelating agent EDTA-2NA 0.05 0.05 0.05 0.05 0.05 0.05 solvent Purified water to 100 to 100 to 100 to 100 to 100 to 100

[Test Example 1] Measurement of micelle size

The prepared Examples and Comparative Examples were diluted in purified water to make a 1% solution, and then, 10 ml of each content was put in a cuvette to prepare a sample. Then, the micellar size of each Example and Comparative Example sample was measured using a Zetasizer Nano ZS90 manufactured by Malvern Instruments. The results are shown in Table 3.

Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 micelle size
(Unit: nm) 14.78 13.82 12.98 6.68 12.39 12.02 12.18 11.87 6.18

As shown in Table 3, as a result of measuring the size of micelles of composition samples containing various surfactants after fixing the content of cocamidopropylbetaine, alkyl polyglucoside, which is a nonionic surfactant, was added to cocamidopropylbetaine. The blended Comparative Example 6 contained cocamidopropylbetaine but showed no change or rather small change in the size of micelles compared to Comparative Example 1 containing no other nonionic surfactant.

However, when cocamide MEA or cocamide methyl MEA, which is a nonionic surfactant, is mixed with cocamidopropyl betaine (Comparative Examples 2 to 5), it can be seen that the size of micelles is about twice that of Comparative Example 1. .

In addition, rather than using Cocamide MEA or Cocamide Methyl MEA alone (Comparative Examples 2 to 5) as a nonionic surfactant used together with cocamidopropyl betaine, Cocamide MEA and Cocamide Methyl MEA When these were used together (Examples 1 to 3), it was confirmed that the size of micelles was further increased. In particular, comparing the size of micelles of Example 1 and Comparative Examples 4 to 5 when the total content of nonionic surfactant is the same as 3.5%, micelles of Example 1 using Cocamide MEA and Cocamide Methyl MEA together It can be seen that the size is increased by 20% or more compared to Comparative Example 4 or Comparative Example 5 in which Cocamide MEA or Cocamide Methyl MEA is used alone.

On the other hand, in Comparative Example 6, the content of the additional surfactant used together with cocamidopropylbetaine was 5% by weight, which is greater than 3.5% by weight of Example, or 1.5 to 3.5% by weight of Comparative Examples 2 to 5, but micelles The size showed small results. From this, it can be seen that the size of micelles is significantly different depending on which type of surfactant is blended rather than the content of the surfactant.

Therefore, in the cleaning composition using cocamidopropyl betaine, when other surfactants are additionally formulated, the effect of increasing the size of micelles when using the nonionic surfactants Cocamide MEA and Cocamide Methyl MEA at the same time was confirmed to be obtainable. In particular, it was confirmed that the most desirable results can be obtained when the mixing ratio of cocamidopropyl betaine, cocamide ME and cocamide methyl MA is 26:1.5:2 by weight.

[Test Example 2] Protein denaturation measurement

The degree of protein denaturation of Examples 1 and Comparative Examples 1 to 5 prepared above was measured by the following method.

(1) 1 g of Zein protein powder (insoluble) was quantified in a 50 ml conical tube, respectively.

(2) The prepared Examples 1 and Comparative Examples 1 to 5 were diluted in purified water to make a 10% solution, and then, each content was prepared by putting 10 ml of each in a cuvette.

(3) After adding (2) to (1) and mixing evenly, it was reacted at 130 rpm on a stirrer for 24 hours.

(4) (3) was centrifuged at 1000 rpm for 3 minutes.

(5) After taking the supernatant of (4) with a 10 ml syringe, it was filtered with a 0.45 μm nylon syringe filter.

(6) Using a 20-fold diluted solution of (5) with purified water as a sample, the denatured protein contained in each sample was quantified through BCA (bicinchoninate) assay.

When a surfactant and a protein are mixed, a difference in the degree of protein denaturation occurs depending on the surfactant mixing ratio of each composition. Accordingly, when the denatured protein is taken from each sample diluted in (5), placed in 96 Well, and reacted with the solution of the BCA assay KIT, the color of the sample in which a lot of the denatured protein is dissolved is changed to dark purple. This was measured by Elisa to quantify the denatured protein. The results are shown in Table 4.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 protein denaturation
(Measured amount of denatured Corn protein (zein protein), μg/ml) 3.6 4.01 3.88 4.39 3.98 4.62

As shown in Table 4, in the case of Example 1 in which cocamide MEA and cocamide methyl MEA were used together with cocamidopropyl betaine, it was confirmed that the degree of protein denaturation was the lowest.

In contrast, the protein denaturation degree of Comparative Example 2 and Comparative Example 4 using cocamide MEA alone was slightly lower than Comparative Example 1 but higher than Example 1, and Comparative Example 3 and Comparative Example 5 using Cocamide MEA alone. The degree of protein denaturation was rather higher than that of Comparative Example 1.

In particular, when comparing the protein denaturation degree of Example 1 and Comparative Examples 4 to 5 when the total content of the nonionic surfactant is the same as 3.5%, the protein of Example 1 using Cocamide MEA and Cocamide Methyl MEA together It can be seen that the degree of denaturation is about 10% lower than Comparative Example 4 in which cocamide MEA was used alone, and 20% or more lower than Comparative Example 5 in which cocamide MEA was used alone.

[Test Example 3] Lipid dissolution measurement

The degree of lipid dissolution of Examples 1 and Comparative Examples 1 to 5 prepared above was measured by the following method.

(1) Prepare a sample prepared by diluting Example 1 and Comparative Examples 1 to 5 in purified water to form a 1% solution.

(2) Make a lipid material containing triglyceride (TGC), oleic acid, and orange oil.

(3) After adding 50 μl of the lipid material using a 200 μl pipette, quickly shake it with a vortex mixer for 10 seconds (Vortexing) and leave it for 1 hour.

(4) Remove the supernatant (oil), measure only 5 ml of the supernatant, and weigh in a Petri dish.

(5) Measure a*(Redness) using a Minolta-CM5 colorimeter.

The results measured by the above measurement method are shown in Table 5 below.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Lipid Elution
(Redness a*) 1.11 1.86 1.87 1.53 1.76 1.63

As shown in Table 5 above, it was confirmed that the amount of lipid elution was the lowest in Example 1 in which cocamide MEA and cocamide methyl MEA were used together with cocamidopropyl betaine.

In contrast, the lipid elution amounts of Comparative Examples 2 and 4 using cocamide MEA alone were similar to or slightly less than those of Comparative Example 1, and the lipid elution amounts of Comparative Examples 3 and 5 using cocamide methyl MEA alone were comparable to those of Comparative Examples. It was less than 1, but more than Example 1.

In particular, when comparing the lipid dissolution of Example 1 and Comparative Examples 4 to 5 when the total content of the nonionic surfactant is the same as 3.5%, the lipid dissolution of Example 1 using cocamide MEA and cocamide methyl MEA together It can be seen that the degree is about 30 to 40% lower than that of Comparative Example 4 or Comparative Example 5 in which cocamide MEA or cocamide methyl MEA was used alone.

[Test Example 4] Eye mucous membrane irritation test (HET-CAM Test)

The eye mucosa irritation test (HET-CAM Test) of Example 1 and Comparative Examples 1 to 5 prepared above was performed in the following manner.

Incubate the fertilized eggs in an automatic incubator for 9 days at a temperature of 37.5±0.5 ℃ and a humidity of 55±7%. On the 10th day of culture, air chambers were observed through the incubation period, and only fertilized eggs with well-developed CAM were selected and used for the experiment. After removing the eggshell using forceps, pour 37℃ sterile physiological saline onto the eggshell membrane so that the eggshell membrane and the CAM can be separated well. Using blunt-tipped forceps, remove the eggshell membrane so as not to injure the blood vessels on the CAM surface to expose the CAM. Among the evaluation methods of the HET-CAM test, the termination response evaluation method was used to determine eye irritation. After reacting the evaluation material on the CAM surface for 30 seconds, the CAM was carefully washed with sterile physiological saline at 37°C, and the degree of blood vessel bleeding, fusion, and coagulation was evaluated at the time point 30 seconds elapsed to measure the stimulation score. Six fertilized eggs were used per test substance, and the evaluation result according to the termination reaction evaluation method was expressed as the sum of each observation item, and was judged by referring to the evaluation criteria table.

The results are shown in Table 6 below.

Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 eye irritation score
(HET-CAM test) 0.08 0.65 0.55 0.45 0.66 0.58

As shown in Table 6, in the case of Example 1 in which cocamide MEA and cocamide methyl MEA were used together with cocamidopropyl betaine, it was confirmed that the degree of eye irritation was very low. Example 1 has a very low eye irritation score of 12 to 14% compared to Comparative Example 4 or Comparative Example 5.

In contrast, in Comparative Example 4 and Comparative Example 5 in which cocamide MEA was used alone, while the total content (3.5%) of the nonionic surfactant was the same as that of Example 1, the degree of irritation of the eye mucosa was It was almost similar to Comparative Example 1. From this, it was predicted that when cocamide MEA or cocamide methyl MEA was used alone, there was little effect of reducing eye irritation, and therefore, the effect of reducing skin irritation was also expected to be less than that of Example 1.

Therefore, when considering the results of Test Examples 1 to 4, if a certain amount of cocamide MEA or cocamide methyl MEA, which is a nonionic surfactant, is used in combination with cocamidopropyl betaine, a betaine-based amphoteric surfactant, , it can be seen that by increasing the micelle size of the cleaning composition containing them, and reducing the protein denaturation and lipid elution of the skin, it can bring about the effect of lowering skin irritation as a whole.

Preferably, when the cocamide MEA and the cocamide methyl MEA are mixed with the cocamidopropyl betaine in a certain amount or in a certain ratio and used together, the micelle size of the cleaning composition containing them is further increased, and the skin By further reducing protein denaturation and lipid elution, it was confirmed that the overall skin irritation improvement effect was very good.

Claims (10)

betaine-based amphoteric surfactants; and
two or more fatty acid alkanolamide-based nonionic surfactants; As a hypoallergenic detergent composition containing as an active ingredient,
The betaine-based amphoteric surfactant is cocamidopropyl betaine,
The fatty acid alkanolamide-based nonionic surfactant is Cocamide MEA and Cocamide Methyl MEA,
The content of the betaine-based amphoteric surfactant is 10 to 30% by weight based on the total weight of the composition,
The content of the fatty acid alkanolamide-based nonionic surfactant is 1.5 to 3.5 wt% based on the total weight of the composition, characterized in that the hypoallergenic detergent composition. The mild detergent composition according to claim 1, wherein the betaine-based amphoteric surfactant and cocamide MEA and cocamide methyl MEA are contained in a weight ratio of 26:1.5:2. According to claim 1, wherein the cleaning composition is a surfactant as a disodium cocoamphodiacetate (Disodium Cocoamphodiacetate), sodium cocoamphoacetate (Sodium Cocoamphoacetate), alkyl polyglucoside (Alkyl Poly Glucoside), Laureth-4 (Laureth-4) ), Disodium Laureth Sulfosuccinate, Sodium Methyl Cocoyl Taurate, Disodium Cocoyl Glutamate, Potassium Cocoyl Glycinate ), characterized in that it further contains at least one kind from the group consisting of, hypoallergenic detergent composition. The mild detergent composition according to any one of claims 1 to 3, wherein the cleaning composition further contains a moisturizing agent, a pH adjusting agent, a chelating agent, and water. According to claim 1, wherein the hypoallergenic is characterized in that the effect according to the increase in the size of micelles, the decrease in protein denaturation and the decrease in lipid dissolution, hypoallergenic detergent composition. delete delete delete delete delete

Images