KR102428554B1 - Additive composition for fertilizer - Google Patents
Additive composition for fertilizer Download PDFInfo
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- KR102428554B1 KR102428554B1 KR1020210129514A KR20210129514A KR102428554B1 KR 102428554 B1 KR102428554 B1 KR 102428554B1 KR 1020210129514 A KR1020210129514 A KR 1020210129514A KR 20210129514 A KR20210129514 A KR 20210129514A KR 102428554 B1 KR102428554 B1 KR 102428554B1
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- South Korea
- Prior art keywords
- stevia
- water
- weight
- leaves
- fertilizer
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 235000009736 ragweed Nutrition 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229960001548 salinomycin Drugs 0.000 description 1
- 235000019378 salinomycin Nutrition 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 150000004354 sesquiterpene derivatives Chemical group 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 235000015096 spirit Nutrition 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/44—Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
Abstract
Description
본 발명은 스테비아를 이용한 사료 및 비료용 첨가제 조성물에 관한 것으로, 더욱 상세하게는 항생제를 사용하지 않고도 가축의 면역력을 증가시킬 수 있고 농작물의 병충해에 대한 저항성을 향상시킬 수 있는 스테비아를 이용한 사료 및 비료용 첨가제 조성물에 관한 것이다. The present invention relates to an additive composition for feed and fertilizer using stevia, and more particularly, feed and fertilizer using stevia, which can increase immunity of livestock without using antibiotics and improve resistance to pests and diseases of crops It relates to an additive composition for
현재 우리의 시대는 농약과 비료의 개발로 농업의 비약적인 발전을 이루었으나 그 반대로 우리의 토양과 우리의 환경은 회복 불능의 상태가 되고 원인을 알 수 없는 병해충과 치료할 수 없는 병으로 고통을 겪고 있다. 이러한 병든 자연환경과 토양을 치유하고 각종 동·식물에게도 면역력을 높여 튼튼하고 싱싱한 먹거리를 제공할 것이 요구되고 있다.In our current era, agriculture has made a leap forward with the development of pesticides and fertilizers, but on the contrary, our soil and our environment are in a state of irreversibility and are suffering from unknown diseases and pests and diseases that cannot be cured. . It is required to provide strong and fresh food by healing the diseased natural environment and soil, and by increasing immunity to various animals and plants.
사료 산업은 식량 산업에 속하며 식품 안전 문제와 직접 연계되어 있고, 사료 안전은 안전한 육류 생산의 기반이며, 사료 안전은 사료 자체 안전뿐만 아니라 사람의 안전과 건강에도 직접적인 영향을 미친다.The feed industry belongs to the food industry and is directly related to food safety issues, feed safety is the basis for safe meat production, and feed safety directly affects not only the safety of feed itself, but also human safety and health.
사료 첨가제는 대상 동물의 특수한 요구를 충족시키기 위하여 일반 사료에 미량으로 첨가되는 물질이며, 사료의 영양학적인 측면에 있어 첨가제의 정확한 의미는 영양소는 아니지만 사료에 첨가했을 때 사료 효율, 생산성 및 동물의 품질을 향상 또는 보존시키는 물질을 말하며, 동물의 생산성과 퍼포먼스를 증가시키기 위해 사용된다.Feed additives are substances added to general feed in trace amounts to meet the special needs of target animals, and in terms of nutrition, the exact meaning of additives is not a nutrient, but when added to feed, feed efficiency, productivity and quality of animals A substance that enhances or preserves animal health and is used to increase animal productivity and performance.
우리나라는 사료첨가용 항생 물질의 사용을 2011년 7월 이후부터 사용 금지하는 조치를 EU에 이어 세계에서 2번째로 이행하여 성장촉진용 항생제(Antibiotics Growth Promoter; AGP)의 대체 물질의 사용에 대한 관심과 연구가 활발히 진행되고 있다.Korea is the second country in the world to implement a measure to ban the use of antibiotics for feed additives after July 2011, following the EU, and interest in the use of alternative substances for antibiotics growth promoters and research is being actively pursued.
이에 많은 성장촉진용 항생제 대체물질들이 시장에 도입되고 있다. 주요 성장촉진용 항생제 대체제로 프로바이오틱스/프리바이오틱스, 효모, 식물추출물, 정유, 유기산, 미네랄, 효소 등이 있는데 우리나라에 일대일로 대체하여 성장촉진용 항생제의 생산성을 낼 수 있는 대체제는 없는 실정이다. 구제역(FMD) 및 조류 독감(AvianInfluenza, AI) 등 바이러스성 질환의 경우에는 전량 외국에서 수입하는 FMD 백신 외에는 적절한 대응 수단이 없는 실정이다.Accordingly, many antibiotic substitutes for growth promotion are being introduced into the market. There are probiotics/prebiotics, yeast, plant extracts, essential oils, organic acids, minerals, and enzymes as major alternatives to antibiotics for growth promotion. In the case of viral diseases such as foot-and-mouth disease (FMD) and avian influenza (AI), there is no adequate countermeasures other than FMD vaccines imported from foreign countries.
최근, 배합사료용 항생제의 사용 금지로 인해 면역증강제에 대한 시장의 요구에 부응하여 허브나 한방약재로 사용되는 식물체 혹은 식물유래 성분들을 활용하는 프리바이오틱스나 항균물질을 만들어 생균제로 활용 가능한 균주에 대한 연구들이 많이 진행되고 있다.Recently, due to the ban on the use of antibiotics for compound feed, in response to the market demand for immune enhancing agents, plants used as herbs or herbal medicines or prebiotics or antibacterial substances using plant-derived ingredients are made to prepare strains that can be used as probiotics. A lot of research is going on.
또한, 현재 농업에서는 농산물 생산을 증가시키기 위하여 화학 비료, 살균제, 살충제, 제초제가 과다하게 사용되고 있다. 이는 질소 기아와 같은 영양 결핍, 토양 오염과 같은 환경 악화, 농업 종사자의 건강 문제, 새로운 병균, 해충의 유입 및 출현, 내성균, 내성 해충의 출현과 같이 농업에 악영향을 주고 있다.In addition, in current agriculture, chemical fertilizers, fungicides, pesticides, and herbicides are excessively used in order to increase agricultural production. This has adverse effects on agriculture, such as nutritional deficiencies such as nitrogen starvation, environmental deterioration such as soil pollution, health problems of agricultural workers, introduction and appearance of new pathogens and pests, and emergence of resistant bacteria and resistant pests.
이와 같이 가축이나 농산물에 첨가되어 면역성 및 병충해에 대한 저항성을 향상시키기 위한 사료첨가제 및 비료 첨가제에 대한 종래기술은 하기와 같다. As described above, the prior art for feed additives and fertilizer additives for improving immunity and resistance to pests and diseases by being added to livestock or agricultural products are as follows.
대한민국 등록특허 제10-0643596호에서는 항산화, 항암, 항균, 항바이러스, 해독 등의 다양한 생리활성을 가진 몰로키아, 인동초 및 고단백의 주정박을 이용하여 돼지의 면역력을 증가시키고 여러 가지 영양소가 축적되게 하여 고품질의 육을 생산할 수 있는 사료 첨가제를 개시하고 있다. 또한, 대한민국 등록특허 제10-1042070호는 광합성 세균 중 로도슈도모나스 루티라 및 이를 이용하여 식물의 성장 촉진, 면역 증강, 잡초 제거, 토양 개선의 효과를 갖는 비료 첨가제를 개시하고 있다. 또한, 대한민국 등록특허 제10-2222811호에서는 유용미생물 배양액, 황칠 추출물, 게르마늄 액비, 콩, 청미래덩굴, 오미자 및 죽엽을 포함하는 혼합물을 발효하여 동, 식물의 면역력을 증강시키는 사료 및 비료 첨가제 조성물을 개시하고 있다.Korean Patent Registration No. 10-0643596 uses Molochia, honeysuckle, and high-protein spirits that have various physiological activities such as antioxidant, anticancer, antibacterial, antiviral, and detoxification to increase immunity of pigs and accumulate various nutrients. Thus, a feed additive capable of producing high-quality meat is disclosed. In addition, Republic of Korea Patent No. 10-1042070 discloses a fertilizer additive having the effects of promoting plant growth, enhancing immunity, removing weeds, and improving soil by using Rhodopseudomonas rutira among photosynthetic bacteria and the same. In addition, Korean Patent Registration No. 10-2222811 discloses a feed and fertilizer additive composition that enhances the immunity of animals and plants by fermenting a mixture containing useful microorganism culture broth, hwangchil extract, germanium liquid fertilizer, soybeans, Smilax china L. is starting
한편, 종래 비료 또는 사료의 제조시 농업·임업·축산업·수산업·제조업 또는 판매업을 영위하는 과정에서 나온 부산물, 예시적으로 가축분 퇴비, 부숙겨, 분뇨잔사, 도축 부산물, 부엽토, 건조축산폐기물 등을 이용하여 사례가 많아지고 있다. 이러한 부산물 비료 또는 사료는 자연순환적인 친환경 농축산업을 수행하는데 있어서 그 효용가치가 매우 크다. On the other hand, in the production of conventional fertilizer or feed, by-products from the process of running agriculture, forestry, livestock, fishery, manufacturing, or sales, for example, livestock meal compost, hulled bran, manure residue, slaughter by-products, humus, dry livestock waste, etc. There are more and more examples of using it. These by-product fertilizers or feeds have a very large utility value in carrying out a natural cycle eco-friendly agricultural and livestock industry.
그러나 이러한 부산물 비료 또는 사료는 악취가 심해 가축이 급이를 거부하거나, 토양에 시비시 민원의 대상이 되는 등의 문제가 있다. However, these by-product fertilizers or feeds have a bad odor, so livestock refuse to feed them, or they are subject to civil complaints when fertilizing the soil.
따라서, 본 발명의 목적은 항생제를 사용하지 않고도 가축의 면역력을 증가시킬 수 있고 농작물의 병충해에 대한 저항성을 향상시킬 수 있는 스테비아를 이용한 사료 및 비료용 첨가제 조성물을 제공하는 데 있다.Accordingly, an object of the present invention is to provide an additive composition for feed and fertilizer using stevia that can increase the immunity of livestock without the use of antibiotics and improve resistance to pests and diseases of crops.
본 발명의 다른 목적은 사료 또는 비료의 악취를 빠르게 제거하는 스테비아를 이용한 사료 및 비료용 첨가제 조성물을 제공하는 데 있다.Another object of the present invention is to provide an additive composition for feed and fertilizer using stevia that quickly removes the odor of feed or fertilizer.
상기한 목적을 달성하기 위한 본 발명의 사료 및 비료용 첨가제 조성물은, 유용미생물 배양액, 스테비아, 황칠 추출물, 도깨비부채 및 행채의 혼합물이 발효된 발효물을 유효성분으로 포함하며, 상기 유용미생물 배양액은 유용미생물(EM) 원액, 당밀 및 물을 혼합하여 배양한 것임을 특징으로 한다. The additive composition for feed and fertilizer of the present invention for achieving the above object includes, as an active ingredient, a fermented product obtained by fermenting a mixture of a useful microorganism culture medium, stevia, hwangchil extract, goblin fan and haengchae, and the useful microorganism culture medium is It is characterized in that it is cultured by mixing useful microorganism (EM) stock solution, molasses and water.
상기 유용미생물 배양액 100중량부에 대하여, 상기 스테비아 0.1~100중량부, 황칠 추출물 0.1~100중량부, 도깨비 부채 잎 0.01~50중량부 및 행채 0.01~50중량부를 포함하는 것이고, 상기 스테비아는, 잎, 줄기 중 1종 이상을 분말화한 분말; 및 잎, 줄기 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고, 상기 황칠 추출물은, 황칠나무의 수액; 및 황칠나무의 수피, 줄기 및 잎 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하며, 상기 도깨비 부채는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고, 상기 행채는, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것임을 특징으로 한다.With respect to 100 parts by weight of the useful microbial culture solution, 0.1 to 100 parts by weight of the stevia, 0.1 to 100 parts by weight of Hwangchil extract, 0.01 to 50 parts by weight of Goblin fan leaf, and 0.01 to 50 parts by weight of Haengchae, the stevia is a leaf , a powder obtained by pulverizing one or more of the stems; and an extract obtained by extracting at least one of leaves and stems using water as an extraction solvent; Including at least one of, the hwangchil extract, sap of hwangchil tree; and extracts obtained by extracting at least one of the bark, stems and leaves of Hwangchil tree using water as an extraction solvent; It contains one or more of the following, the goblin fan is a powder obtained by pulverizing leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; Containing one or more of, the haengchae is a powder made from a powdered plant; and extracts obtained by extracting whole plants using water as an extraction solvent; It is characterized in that it includes at least one of them.
상기 혼합물은, 분홍애기풀 0.01~50중량부, 카스타놉시스 에키노카르파 0.01~50중량부를 더 포함하고, 상기 분홍애기풀은, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것이며, 상기 카스타놉시스 에키노카르파는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것임을 특징으로 한다.The mixture further comprises 0.01 to 50 parts by weight of chrysanthemum, 0.01 to 50 parts by weight of Castanopsis echinocarpa, wherein the rhododendron is a powder obtained by pulverizing the outpost; and extracts obtained by extracting whole plants using water as an extraction solvent; It will include one or more of the following, the Castanopsis echinocarpa is a powder obtained by pulverizing leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; It is characterized in that it includes at least one of them.
상기 유효성분으로 옥수수속대의 발효추출물을 더 포함하되, 상기 옥수수속대의 발효추출물은 옥수수속대에 락토바실러스 람노서스(Lactobacillus rhamnosus)를 접종하여 발효하고, 이를 물을 추출용매로 하여 추출한 추출물인 것을 특징으로 한다.Further comprising a fermentation extract of corncobs as the active ingredient, wherein the fermented extract of corncobs is an extract obtained by inoculating and fermenting corncobs with Lactobacillus rhamnosus, and using water as an extraction solvent. do it with
본 발명에 의하면, 동, 식물의 면역력을 증강시켜 그 수확량 및 생산량을 늘릴 수 있는 것은 물론, 사료용 항생제를 대체할 수 있어 고급 브랜드의 축산물을 생산할 수 있다는 장점이 있다. 아울러, 각종 부산물 사료 또는 부산물 비료의 악취를 빠르게 제거할 수 있다는 장점이 있다.According to the present invention, it is possible to enhance the immunity of animals and plants to increase the yield and production, as well as to replace antibiotics for feed, so that high-end brand livestock products can be produced. In addition, there is an advantage that can quickly remove the odor of various by-product feed or by-product fertilizer.
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명에 의한 사료 및 비료용 첨가제 조성물은, 유용미생물 배양액, 스테비아, 황칠 추출물, 도깨비부채 및 행채의 혼합물이 발효된 발효물을 유효성분으로 포함하는 것을 특징으로 한다.The additive composition for feed and fertilizer according to the present invention is characterized in that it comprises, as an active ingredient, a fermented product of a mixture of useful microorganism culture broth, stevia, hwangchil extract, goblin fan and haengchae.
유용미생물(Effective Microoganims; EM)이란 광합성 세균과 유산균, 방선균, 사상균, 효모균 등의 다양한 유용 미생물을 단독 또는 둘 이상 포함하도록 배양한 미생물제재이다. 이러한 유용미생물은 수처리, 대기처리 분야뿐 아니라 농업, 축산업, 수산업, 식품, 화장품 등에 널리 활용되고 있고, 악취 제거, 수질 정화, 금속과 식품의 산화 방지, 남은 음식물 발효 등에 탁월한 효과가 있는 것으로 알려져 있다.Effective microorganisms (EM) are microbial preparations cultured to contain single or two or more of various useful microorganisms such as photosynthetic bacteria, lactic acid bacteria, actinomycetes, filamentous fungi, and yeasts. These useful microorganisms are widely used not only in water treatment and air treatment fields, but also in agriculture, livestock industry, fishery industry, food, and cosmetics, and are known to have excellent effects in removing odors, purifying water, preventing oxidation of metals and foods, and fermenting leftover food. .
상기 유용미생물은 강력한 항산화 작용, 고분자 물질의 분해를 통한 저분자화, 중금속의 이온화 방지효과 및 친환경성을 가지며, 유용미생물을 구성하는 혐기성균은 황화수소, 암모니아 등을 악취가 없는 물질로 변화시키고, 항산화 효과와 함께 산소를 생성하며, 호기성균은 유해균을 억제 및 퇴치하는 것으로 밝혀짐에 따라 각종 제품의 원료로 각광받고 있다. 뿐만 아니라, 상기 유용미생물의 경우 일체의 합성화학물질을 사용하지 않고 천연재료로만 제조됨은 물론이고, 생리활성물질을 생성하여 사용 후 생태계에 잔류하더라도 유익한 작용을 하므로, 환경친화적인 원료로서 더욱 주목된다.The useful microorganisms have a strong antioxidant action, low molecular weight through decomposition of high molecular substances, ionization prevention effect of heavy metals, and eco-friendliness, and anaerobes constituting useful microorganisms change hydrogen sulfide, ammonia, etc. It produces oxygen with the effect, and aerobic bacteria are in the spotlight as a raw material for various products as it has been found to inhibit and combat harmful bacteria. In addition, in the case of the useful microorganisms, not only are they manufactured only with natural materials without using any synthetic chemicals, but they also have a beneficial effect even if they remain in the ecosystem after use by generating physiologically active substances, so it is more noteworthy as an environmentally friendly raw material. .
본 발명에서 사용되는 유용미생물 배양액은 효모, 유산균 및 광합성 세균을 필수적으로 포함하는 유용미생물을 배양한 용액을 의미하는 것으로, 이러한 유용미생물은 시중에서 시판되는 EM 원액을 구입하여 사용할 수 있으며, 효모, 유산균 및 광합성 세균을 필수적으로 포함하는 유용미생물이면 제한 없이 사용할 수 있다. 상기 유용미생물의 배양액은 상기 유용미생물을, 당밀 및 물과 혼합하여 배양함으로써 제조할 수 있는바, 이에 대해서는 하기 제조방법에서 더욱 상세히 설명한다. The useful microorganism culture solution used in the present invention refers to a solution in which useful microorganisms essentially containing yeast, lactic acid bacteria and photosynthetic bacteria are cultured, and these useful microorganisms can be used by purchasing commercially available EM stock solutions, yeast, As long as it is useful microorganisms essentially including lactic acid bacteria and photosynthetic bacteria, it can be used without limitation. The culture solution of the useful microorganisms can be prepared by mixing and culturing the useful microorganisms with molasses and water, which will be described in more detail in the following preparation method.
본 발명은 이러한 유용미생물 배양액을 통해 스테비아, 황칠 추출물, 도깨비부채, 행채 등을 발효함으로써, 유효성분의 함량을 극대화하고, 흡수율을 높여준다. 아울러, 본 발명의 사료 및 비료의 첨가제 조성물은, 유용미생물 배양액의 사용으로 미생물의 활동을 최대화하고, 해로운 세균의 발육을 억제하며, 미량원소를 공급하여, 작물 및 가축의 건강한 성장을 돕는 것이다. The present invention maximizes the content of active ingredients and increases the absorption rate by fermenting stevia, hwangchil extract, goblin fan, haengchae, etc. through such a useful microorganism culture medium. In addition, the additive composition of feed and fertilizer of the present invention maximizes the activity of microorganisms by using a useful microbial culture medium, inhibits the growth of harmful bacteria, and supplies trace elements to help healthy growth of crops and livestock.
상기 스테비아(stevia)는 카로틴, 비타민, 단백질, 칼슘, 탄수화물, 글루타민, 유기탄소 등의 인체에 유익한 많은 영양소와 미네랄이 함유되어 있으며, 잎과 줄기에 설탕의 200~300배에 달하는 당도를 가진 저칼로리 감미물질이 포함되어 있어 이를 비료 첨가제로 사용할 경우 수확증대, 뿌리활착, 발근촉진, 당도증대, 신선도 증가 및 유효미생물의 활력으로 병충해 예방효과 등의 효과를 가지며, 사료 첨가제로 사용할 경우 가축의 생장촉진, 면역력 증강 등의 효과를 갖는다. 다만, 이러한 스테비아를 그대로 사용하거나 단순 추출하여 사용할 경우, 그 흡수율이 미약한바, 이를 유용미생물 배양액에 의해 발효시킴으로써, 흡수율을 높여 우수한 작용효과를 갖도록 하는 것이다. The stevia contains many nutrients and minerals beneficial to the human body, such as carotene, vitamins, protein, calcium, carbohydrates, glutamine, organic carbon, etc. Since it contains sweeteners, when used as a fertilizer additive, it has effects such as increasing harvest, rooting, rooting, increasing sugar content, increasing freshness, and preventing pests and pests with the vitality of effective microorganisms. When used as a feed additive, it promotes the growth of livestock. , immunity enhancement, etc. However, when using such stevia as it is or simply extracting it, the absorption rate is weak, and it is fermented with a useful microorganism culture to increase the absorption rate to have an excellent action effect.
상기 스테비아는, 잎, 줄기 중 1종 이상을 분말화한 분말; 및 잎, 줄기 중 1종 이상을 물을 추출용매로 하여 추출한 추출물 중 1종 이상을 포함하는 것을 의미한다. 이때, 그 추출방법은 제한하지 않는바, 이 기술이 속하는 분야에서 공지된 방법에 의하면 족하다. 또한, 그 분말 입도 역시 제한하지 않는다.The stevia is a powder obtained by pulverizing one or more of leaves and stems; and one or more of the extracts extracted by using water as an extraction solvent for one or more of the leaves and stems. At this time, the extraction method is not limited, and it is sufficient according to a method known in the field to which this technology belongs. In addition, the powder particle size is also not limited.
상기 황칠 추출물은 안식향을 함유하여 약리효과가 탁월한바, 항암, 항산화 효과, 간세포재생, 당뇨치료, 경조직 세포 재생 등에 효과적인 것으로 밝혀졌다. 상기 황칠의 성분은 사포닌과 정유(精油)성분으로서 주성분은 sesqui-terpene계의 dendropanoxide이며 그 외에 알콜 및 에테르 등이 함유되어 있다. 즉 황칠에는 α-Cubebene, β-Elemene, β-Selinene, β-Selinene, α-Muurolene Germacrene D, β-Sitosterol, β-Sitosterol 성분이 함유되어 있어서, 고혈압, 중풍, 치매 예방 등에 효과가 있는 것으로 보고되고 있으며, 이외에도 항균 효과, 소화기질환 치료, 강장 효과, 우울증 및 스트레스 해소 등에 효과적인 것으로 알려져 있다. The hwangchil extract contains benzoin and has excellent pharmacological effects, and was found to be effective in anticancer, antioxidant effects, liver cell regeneration, diabetes treatment, hard tissue cell regeneration, and the like. The components of the hwangchil are saponin and essential oil, and the main component is sesqui-terpene-based dendropanoxide, and in addition, alcohol and ether are contained. That is, Hwangchil contains α-Cubebene, β-Elemene, β-Selinene, β-Selinene, α-Muurolene Germacrene D, β-Sitosterol, and β-Sitosterol. In addition, it is known to be effective in antibacterial effect, treatment of digestive diseases, tonic effect, and relieving depression and stress.
본 발명에서 상기 황칠 추출물은 유용미생물 배양액에 의해 발효되어, 각종 활성성분이 극대화되고, 작물 및 가축에의 흡수율 역시 극대화됨으로써, 면역력을 현저히 향상키는 효과를 갖는다.In the present invention, the hwangchil extract is fermented by a culture medium of useful microorganisms, and various active ingredients are maximized, and the absorption rate in crops and livestock is also maximized, thereby significantly improving immunity.
상기 황칠 추출물은, 황칠나무(Dendropanax morbifera)의 수피에 상처를 내서 분비되는 황색의 수액; 및 황칠나무의 수피, 줄기 및 잎 중 1종 이상을 물을 추출용매로 하여 추출한 추출물 중 1종 이상을 포함하는 것을 의미한다. 이때, 그 추출방법은 제한하지 않는바, 이 기술이 속하는 분야에서 공지된 방법에 의하면 족하다. 또한, 그 분말 입도 역시 제한하지 않는다.The hwangchil extract, the yellow sap secreted by making a wound on the bark of the hwangchil tree (Dendropanax morbifera); And it means to include one or more of the extracts extracted by using water as an extraction solvent of one or more of the bark, stem and leaves of Hwangchil tree. At this time, the extraction method is not limited, and it is sufficient according to a method known in the field to which this technology belongs. In addition, the powder particle size is also not limited.
상기 도깨비부채(Rodgersia podophylla)는 쌍떡잎식물 장미목 범의귀과의 여러해살이풀로서 5개로 갈라진 잎의 모양이 마치 도깨비가 사용하는 부채와 같다고 하여 도깨비부채라고 부르며, 산우, 작합산, 수레부채라고도 한다.The dokkaebi fan (Rodgersia podophylla) is a dicotyledonous perennial plant of the Rosaceae family of the order Rosaceae, and the shape of the leaf divided into five is like a fan used by a goblin, so it is called a dokkaebi fan, and is also called sanwoo, jakhapsan, and wagon fan.
본 발명에서 상기 도깨비부채는 그 잎을 분말화한 것; 및 전초를 물을 추출용매로 하여 추출한 추출물 중 1종 이상을 포함하는 것을 의비하는바, 작물 및 가축의 생육을 돕는 것은 물론, 사료 및 비료의 악취 제거를 돕는다. 이때, 그 추출방법은 제한하지 않는바, 이 기술이 속하는 분야에서 공지된 방법에 의하면 족하다. 또한, 그 분말 입도 역시 제한하지 않는다.In the present invention, the goblin fan is a powdered leaf; And it is meant to include one or more of the extracts extracted by using water as an extraction solvent from the forage, helping the growth of crops and livestock, as well as helping to remove the odor of feed and fertilizer. At this time, the extraction method is not limited, and it is sufficient according to a method known in the field to which this technology belongs. In addition, the powder particle size is also not limited.
상기 행채는 학명이 Nymphoides peltata (JGGmelin) Kuntze로서 조름나물과(Menyanthaceae)에 속하는 수년생 수초이며 식물명은 노랑어리연꽃이다.The haengchae has a scientific name of Nymphoides peltata (JGGmelin) Kuntze, and is an aquatic perennial plant belonging to the Menyanthaceae family, and the botanical name is Yellow Lotus.
본 발명에서 상기 행채는 그 전초를 분말화한 것; 및 전초를 물을 추출용매로 하여 추출한 추출물 중 1종 이상을 포함하는 것을 의비하는바, 작물 및 가축의 생육을 돕는 것은 물론, 사료 및 비료의 악취 제거를 돕는다. 이때, 그 추출방법은 제한하지 않는바, 이 기술이 속하는 분야에서 공지된 방법에 의하면 족하다. 또한, 그 분말 입도 역시 제한하지 않는다.In the present invention, the haengchae is a powdered plant; And it is meant to include one or more of the extracts extracted by using water as an extraction solvent from the forage, helping the growth of crops and livestock, as well as helping to remove the odor of feed and fertilizer. At this time, the extraction method is not limited, and it is sufficient according to a method known in the field to which this technology belongs. In addition, the powder particle size is also not limited.
본 발명에 의한 첨가제 조성물은, 상기 유용미생물 배양액 100중량부에 대하여, 상기 스테비아 0.1~100중량부, 황칠 추출물 0.1~100중량부, 도깨비 부채 잎 0.01~50중량부 및 행채 0.01~50중량부를 포함하는 혼합물을 발효한 발효물을 유효성분으로 포함하는 것으로, 이러한 발효 과정을 통해 각종 영양성분의 흡수율을 높이고, 유용물질의 함량을 극대화하여 식물 및 가축의 면역력 향상 및 생육을 돕는 것이다.The additive composition according to the present invention contains 0.1 to 100 parts by weight of stevia, 0.1 to 100 parts by weight of hwangchil extract, 0.01 to 50 parts by weight of goblin fan leaves, and 0.01 to 50 parts by weight of haengchae with respect to 100 parts by weight of the useful microorganism culture solution. It contains a fermented product of a mixture of
한편, 상기 혼합물은, 분홍애기풀 0.01~50중량부, 카스타놉시스 에키노카르파 0.01~50중량부를 더 포함하는 것이 바람직하다.On the other hand, it is preferable that the mixture further comprises 0.01 to 50 parts by weight of chrysanthemum plant, 0.01 to 50 parts by weight of Castanopsis echinocarpa.
상기 분홍애기풀(Binghamia californica J. Agardh)은 홍조식물 분홍치목 마디잘록이과의 바닷말로, 조간대 하부에 자란다. 식물체는 막질이며 분홍색으로 끈 모양의 가는 뿌리에서 납작한 줄기가 여러 개 나온다. 이리저리 굽고 서로 붙으며 얽혀서 덩어리로 된다. 줄기는 두 갈래로 가지를 낸다. 구술모양의 세포가 2~3겹으로 피층을 이루고 수조직은 둥근 세포와 막대 모양의 세포가 어우러져 양쪽 피층조직을 연결한다.The pink algae grass (Binghamia californica J. Agardh) is a sea horse of the red algae plant of the genus Pink algae, and grows in the lower intertidal zone. The plant is membranous, and several flat stems come out of thin pink, string-shaped roots. They are baked, sticking to each other, and entangled to form a lump. The stem is bifurcated. Spherical cells form the cortex in 2-3 layers, and in the male tissue, round cells and rod-shaped cells combine to connect both cortical tissues.
상기 분홍애기풀은, 그 전초를 분말화한 분말; 및 전초를 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것을 의미하는 것으로, 각종 영양성분을 공급하여 가축 및 작물의 성장을 촉진한다. The pink rosemary is a powder obtained by pulverizing the outpost; and extracts extracted by using the whole plant as an extraction solvent; It means to include one or more of them, and promotes the growth of livestock and crops by supplying various nutrients.
상기 카스타놉시스 에키노카르파(Castanopsis echinocarpa)는 메밀잣밤나무속(카스타놉시스, Castanopsis)에 속한 종으로써 상록 활엽 교목이다. 한국, 중국, 일본 및 아열대에서 서식한다. The Castanopsis echinocarpa is a species belonging to the genus Buckwheat chestnut (Castaopsis, Castanopsis) and is an evergreen broad-leaved tree. It lives in Korea, China, Japan and the subtropics.
상기 카스타놉시스 에키노카르파는, 그 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것을 의미하는 것으로, 각종 영양성분을 공급하여 가축 및 작물의 성장을 촉진한다. The Castanopsis echinocarpa is a powder obtained by pulverizing the leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; It means to include one or more of them, and promotes the growth of livestock and crops by supplying various nutrients.
또한, 본 발명의 첨가제 조성물은 상기 유효성분으로 옥수수속대의 발효추출물을 더 포함함이 바람직하다. In addition, it is preferable that the additive composition of the present invention further comprises a fermented extract of corncob as the active ingredient.
상기 옥수수속대의 발효추출물은 악취를 갖는 부산물 비료 또는 사료의 냄새를 빠르게 제거해주는 역할을 하면서도, 가축 및 작물의 생육을 돕는 역할을 한다.The fermented extract of corncob serves to quickly remove the smell of by-product fertilizer or feed having a bad odor, while helping the growth of livestock and crops.
이때, 상기 혼합물의 발효물과 상기 옥수수속대의 발효추출물 간의 배합비는 제한하지 않는데, 1:0.1~0.5 중량비 정도면 바람직하다.At this time, the mixing ratio between the fermented product of the mixture and the fermented extract of corncob is not limited, but preferably 1:0.1 to 0.5 by weight.
상기 옥수수속대의 발효추출물은 옥수수속대에 락토바실러스 람노서스(Lactobacillus rhamnosus)를 접종하여 발효하고, 이를 물을 추출용매로 하여 추출한 발효추출물을 의미한다. The fermentation extract of corncobs refers to a fermentation extract extracted by inoculating and fermenting corncobs with Lactobacillus rhamnosus, and using this as an extraction solvent.
그 구체적인 방법은 옥수수속대에 물을 1:0.5~1 부피비로 혼합하고, 이에 락토바실러스 플란타럼 균주를 1~10(v/v)%로 접종한 후, 35~38℃의 혐기적 조건에서 60~80시간 발효시키고, 이에 5~20중량배의 물를 첨가한 후, 80~100℃에서 1~5시간 동안 추출, 여과 및 건조하여 제조될 수 있다. 상기 여과, 농축 및 건조방법은 당업계에서 공지된 다양한 방법을 이용함은 당연하다.The specific method is to mix water in a corncob in a volume ratio of 1:0.5 to 1, inoculate the Lactobacillus plantarum strain at 1 to 10 (v/v)%, and then in anaerobic conditions at 35 to 38 ° C. Fermented for 60 to 80 hours, 5 to 20 times by weight of water is added thereto, and extracted, filtered and dried at 80 to 100° C. for 1 to 5 hours. It is natural that the filtration, concentration and drying methods use various methods known in the art.
이하, 본 발명에 의한 비료 및 사료 첨가제 조성물의 제조방법에 대하여 상세히 설명한다.Hereinafter, a method for producing a fertilizer and feed additive composition according to the present invention will be described in detail.
본 발명에 의한 비료 및 사료 첨가제 조성물의 제조방법은, 유용미생물(EM) 원액, 당밀 및 물을 혼합하여 배양함으로써, 유용미생물 배양액을 제조하는 단계와, 상기 유용미생물 배양액에 스테비아, 황칠 추출물, 도깨비부채 및 행채를 혼합하는 단계와, 상기 혼합된 혼합물을 발효하는 단계를 포함하는 것을 특징으로 한다. The manufacturing method of the fertilizer and feed additive composition according to the present invention includes the steps of preparing a useful microbial culture solution by mixing and culturing a useful microorganism (EM) stock solution, molasses and water, and stevia, hwangchil extract, goblin in the useful microorganism culture solution It characterized in that it comprises the step of mixing the fan and the haengchae, and fermenting the mixed mixture.
먼저, 유용미생물 배양액을 제조한다.First, a culture medium of useful microorganisms is prepared.
상기 유용미생물 배양액은 당밀 100중량부에 대하여, EM 원액 0.1~0.5중량부 및 물 5~20중량부를 혼합하고, 25~30℃에서 4~7일간 배양하여 제조할 수 있다. 상기 당밀은 비정제 사탕수수당으로, 정제당보다 미네랄이 많으며, EM 원액의 배양을 촉진시켜 주고, 면역력 강화, 에너지 증강 등의 효과를 부여한다.The useful microorganism culture solution can be prepared by mixing 0.1 to 0.5 parts by weight of an EM stock solution and 5 to 20 parts by weight of water with respect to 100 parts by weight of molasses, and culturing at 25 to 30° C. for 4 to 7 days. The molasses is unrefined sugar cane sugar, contains more minerals than refined sugar, promotes the culture of EM stock solution, and imparts effects such as strengthening immunity and enhancing energy.
다음으로, 상기 유용미생물 배양액에 스테비아, 황칠 추출물, 도깨비부채 및 행채를 혼합한다. Next, stevia, hwangchil extract, goblin fan and haengchae are mixed with the useful microorganism culture solution.
이때, 그 혼합비는 상기 유용미생물 배양액 100중량부에 대하여, 상기 스테비아 0.1~100중량부, 황칠 추출물 0.1~100중량부, 도깨비부채 0.1~50중량부 및 행채 0.1~50중량부임이 바람직하다.At this time, the mixing ratio is preferably 0.1 to 100 parts by weight of stevia, 0.1 to 100 parts by weight of hwangchil extract, 0.1 to 50 parts by weight of goblin fan, and 0.1 to 50 parts by weight of haengchae with respect to 100 parts by weight of the useful microorganism culture solution.
아울러, 상기 스테비아는, 잎, 줄기 중 1종 이상을 분말화한 분말; 및 잎, 줄기 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고, 상기 황칠 추출물은, 황칠나무의 수액; 및 황칠나무의 수피, 줄기 및 잎 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하며, 상기 도깨비 부채는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고, 상기 행채는, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것으로, 그 추출방법은 물론, 분말의 입도 등은 제한하지 않는다.In addition, the stevia is a powder obtained by pulverizing one or more of leaves and stems; and an extract obtained by extracting at least one of leaves and stems using water as an extraction solvent; Including at least one of, the hwangchil extract, sap of hwangchil tree; and extracts obtained by extracting at least one of the bark, stems and leaves of Hwangchil tree using water as an extraction solvent; It contains one or more of the following, the goblin fan is a powder obtained by pulverizing leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; Containing one or more of, the haengchae is a powder made from a powdered plant; and extracts obtained by extracting whole plants using water as an extraction solvent; By including one or more of them, the extraction method, as well as the particle size of the powder, etc. are not limited.
또한, 이 단계에서 필요에 따라 분홍애기풀 0.01~50중량부 및 카스타놉시스 에키노카르파 0.01~50중량부를 더 혼합할 수 있다. In addition, in this step, if necessary, 0.01 to 50 parts by weight of chrysanthemum and 0.01 to 50 parts by weight of Castanopsis echinocarpa may be further mixed.
상기 분홍애기풀은, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것이며, 상기 카스타놉시스 에키노카르파는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것으로, 그 추출방법은 물론, 분말의 입도 등은 제한하지 않는다.The pink rhizome is a powder obtained by pulverizing the outpost; and extracts obtained by extracting whole plants using water as an extraction solvent; It will include one or more of the following, the Castanopsis echinocarpa is a powder obtained by pulverizing leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; By including one or more of them, the extraction method, as well as the particle size of the powder, etc. are not limited.
그리고 상기 혼합액을 25~30℃로 2~7일간 발효함으로써, 본 발명에 의한 첨가제 조성물을 제조한다. 상기 발효를 통해 각종 활성성분의 함량 증가시키고, 흡수율을 높임으로써, 작물 및 가축의 생육을 돕는 것이다.And by fermenting the mixture at 25 to 30 ° C. for 2 to 7 days, an additive composition according to the present invention is prepared. By increasing the content of various active ingredients through the fermentation and increasing the absorption rate, it is to help the growth of crops and livestock.
아울러, 옥수수속대 발효추출물을 더 혼합하고자 할 경우, 상기 발효된 발효물에 옥수수속대의 발효추출물을 혼합하여 첨가제 조성물의 제조를 완료한다.In addition, when it is desired to further mix the fermented corncob extract, the preparation of the additive composition is completed by mixing the fermented corncob extract with the fermented fermented product.
이때, 상기 옥수수속대의 발효추출물은 앞서 설명된 바와 같이, 옥수수속대에 락토바실러스 람노서스(Lactobacillus rhamnosus)를 접종하여 발효하고, 이를 물을 추출용매로 하여 추출한다.At this time, as described above, the fermented extract of corncobs is fermented by inoculating Lactobacillus rhamnosus into the corncobs, and it is extracted using water as an extraction solvent.
상기와 같이 제조되는 본 발명의 첨가제 조성물은 일반적인 사료 또는 비료에 혼합되어 사용되거나, 물에 희석하여 단독으로 사용할 수 있는바, 그 사용방법을 제한하지 않는다. 예시적으로, 본 발명의 첨가제 조성물을 물로 50~200배 정도 희석하여 작물에 관주식 또는 엽면식으로 살포하거나, 상기 첨가제 조성물을 일반적인 비료에 0.5~2중량% 내로 혼합하여 관주식 또는 엽면식 살포하거나, 가축용 사료에 0.5~2중량%로 첨가하거나, 물에 0.5~2중량%로 첨가하여 가축에게 복용시킬 수 있는 것으로, 그 사용을 제한하지 않는다. The additive composition of the present invention prepared as described above may be mixed with general feed or fertilizer, or used alone after being diluted in water, and the method of use thereof is not limited. Illustratively, by diluting the additive composition of the present invention by 50 to 200 times with water and spraying the crops in a irrigation or foliar manner, or by mixing the additive composition in 0.5 to 2% by weight in a general fertilizer and spraying it in a irrigation or foliar manner. Or, it can be added to livestock feed at 0.5 to 2% by weight, or added to water at 0.5 to 2% by weight to be taken by livestock, and its use is not limited.
아울러, 적용되는 작물의 종류 및 가축의 종류 역시 제한하지 않는바, 포도, 감귤, 보리싹, 고추, 상추, 배추 등의 식물은 물론, 닭, 돼지, 소, 오리 등의 가축에 적용가능하다.In addition, the types of crops and livestock to be applied are also not limited, and it is applicable to plants such as grapes, tangerines, barley sprouts, peppers, lettuce, and Chinese cabbage, as well as livestock such as chickens, pigs, cattle, and ducks.
상기와 같은 본 발명의 첨가제 조성물을 적용할 경우, 가축 및 작물의 면역력을 향상시켜 질병을 예방하고, 해충, 바이러스의 발생을 방지하는 것은 물론, 생육을 도와 수확량을 늘릴 수 있다는 장점이 있다. When the additive composition of the present invention as described above is applied, it is possible to improve immunity of livestock and crops to prevent diseases, and to prevent the occurrence of pests and viruses, as well as to help growth and increase the yield.
이하, 본 발명의 실시예에 대해 상세히 설명한다. Hereinafter, embodiments of the present invention will be described in detail.
(실시예 1)(Example 1)
당밀 4,000kg, EM 원액 5kg, 물 500kg을 혼합하고, 25~30℃에서 5일간 배양하여 EM 배양액을 제조하였다. 4,000 kg of molasses, 5 kg of EM stock solution, and 500 kg of water were mixed, and cultured at 25 to 30° C. for 5 days to prepare an EM culture solution.
상기 EM 배양액에 스테비아 20kg, 황칠 추출물 20kg, 도깨비나무 10kg 및 행채 10kg을 혼합하고, 25~30℃에서 다시 3일간 발효하여 첨가제 조성물을 제조하였다.20 kg of stevia, 20 kg of hwangchil extract, 10 kg of goblin and 10 kg of haengchae were mixed in the EM culture medium, and fermented at 25 to 30° C. for 3 days to prepare an additive composition.
이때, 상기 스테비아는 스테비아 잎의 건조분말을 사용하였고, 상기 황칠 추출물은 황칠나무의 수피에 5중량배의 물을 가하고, 90~100℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였고, 상기 도깨비나무는 도깨비나무 잎의 건조분말을, 상기 행채는 전초의 건조분말을 사용하였다.At this time, the stevia used the dry powder of stevia leaves, and the hwangchil extract was used by adding 5 weight times water to the bark of the hwangchil tree, extracting it at 90-100° C. for 10 hours, filtration, concentration and drying, For the Goblin tree, the dried powder of the leaves of the Goblin tree was used, and for the haengchae, the dried powder of the outpost was used.
(실시예 2)(Example 2)
실시예 1과 동일하게 실시하되, 상기 스테비아는 그 잎에 5중량배의 물을 가하고, 90℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였고, 상기 도깨비나무는 그 잎에 5중량배의 물을 가하고, 90℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였으며, 상기 행채는 그 전초에 5중량배의 물을 가하고, 90℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였다.It was carried out in the same manner as in Example 1, except that the stevia was used by adding 5 times by weight of water to the leaves, extracting at 90° C. for 10 hours, filtration, concentration, and drying, and the stevia was 5 times by weight on the leaves. of water was added, extracted at 90 ° C. for 10 hours, filtered, concentrated and dried, and the haengchae was used by adding 5 times by weight of water to the forearm, and extracted at 90 ° C. for 10 hours, followed by filtration, concentration and drying. was used.
(실시예 3)(Example 3)
실시예 2와 동일하게 실시하되, 상기 EM 배양액에 스테비아, 황칠 추출물, 도깨비나무, 행채를 혼합할 시, 분홍애기풀 10kg, 카스타놉시스 에키노카르파 10kg을 더 혼합하였다. It was carried out in the same manner as in Example 2, but when stevia, hwangchil extract, goblin tree, and haengchae were mixed in the EM culture medium, 10 kg of pink ragweed and 10 kg of Castanopsis echinocarpa were further mixed.
이때, 분홍애기풀은 그 전초에 5중량배의 물을 가하고, 90℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였으며, 상기 카스타놉시스 에키노카르파는 그 잎에 5중량배의 물을 가하고, 90℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 사용하였다.At this time, P. oleracea was added 5 times by weight of water to the outpost, extracted at 90° C. for 10 hours, filtered, concentrated and dried, and the Castanopsis echinocarpa was 5 times by weight of water on the leaves. After addition, extraction was performed at 90° C. for 10 hours, followed by filtration, concentration and drying.
(실시예 4)(Example 4)
실시예 1과 동일하게 실시하되, 상기 제조된 발효물에 옥수수속대의 발효추출물을 1:0.2 중량비로 혼합하였다.It was carried out in the same manner as in Example 1, except that the prepared fermented product was mixed with a fermented extract of corncob in a weight ratio of 1:0.2.
이때, 상기 옥수수속대의 발효추출물은 옥수수속대를 100mesh 정도로 분쇄한 후, 이에 물을 1:1 부피비로 혼합하였다. 그리고 이에 락토바실러스 플란타럼 균주를 3(v/v)%로 접종하고, 37℃의 혐기적 조건에서 72시간 발효시켰다. 그리고 이 발효물에 5중량배의 물을 가한 후, 90~100℃에서 10시간 추출한 후, 여과, 농축 및 건조하여 제조하였다.At this time, the fermented extract of corncobs was ground corncobs to about 100mesh, and then water was mixed thereto in a 1:1 volume ratio. Then, the Lactobacillus plantarum strain was inoculated at 3 (v/v)%, and fermented for 72 hours under anaerobic conditions at 37°C. Then, 5 times by weight of water was added to the fermented product, followed by extraction at 90 to 100° C. for 10 hours, followed by filtration, concentration and drying.
상기 락토바실러스 플란타럼 균주는 Lactobacillus plantarum SRCM 9균주를 MRS 아가 플레이트(Difco)에 계대배양 한 뒤, 100ml MRS 배지(Difco)에 접종하여 진탕배양기(37℃, 160rpm)에서 24시간 동안 본 배양하여 균수를 1.0×106CFU/ml 수준으로 조절하여 사용하였다.The Lactobacillus plantarum strain was subcultured on an MRS agar plate (Difco) with 9 strains of Lactobacillus plantarum SRCM, and then inoculated into 100ml MRS medium (Difco) and incubated for 24 hours in a shaking incubator (37°C, 160rpm). The number of bacteria was adjusted to a level of 1.0×10 6 CFU/ml and used.
(시험예 1)(Test Example 1)
실시예 1 내지 4에 의한 첨가제 조성물의 항균 능력을 보기 위하여, 대장균 O-157 및 살모넬라 엔테리티디스(Salmonella enteritidis)에 대하여 항균 활성 실험을 하였다.In order to see the antibacterial ability of the additive composition according to Examples 1 to 4, E. coli O-157 and Salmonella enteritidis (Salmonella enteritidis) were subjected to an antibacterial activity test.
시험군은 상기 균주를 식염수에 접종한 후 실시예 1 내지 4에 따른 첨가제 조성물 1% 수용액을 첨가하여 24시간 동안 배양시킨 후 선택 배지에 도말하고, 도말 후 48시간 동안 배양한 후에 각 균주의 개체수를 측정하였다. 대조군은 첨가제 조성물 수용액을 첨가하지 않았다. 그 결과를 표 1에 나타내었다.After inoculating the strain in saline, the test group was cultured for 24 hours by adding a 1% aqueous solution of the additive composition according to Examples 1 to 4, spread on a selective medium, and after culturing for 48 hours after plating, the number of individuals of each strain was measured. The control group did not add an aqueous additive composition solution. The results are shown in Table 1.
상기 표 1에서와 같이, 본 발명에 의한 실시예 1 내지 4는 병원성 세균의 증식을 억제할 수 있으며, 우수한 항균력을 가져 항생제 대체 물질로 사용이 가능함을 확인할 수 있었다.As shown in Table 1, Examples 1 to 4 according to the present invention can inhibit the growth of pathogenic bacteria, have excellent antibacterial activity, it was confirmed that it can be used as an antibiotic replacement material.
(시험예 2)(Test Example 2)
실시예 1 내지 4의 첨가제 조성물을 육계용 배합사료에 1중량%로 혼합하여 사료를 제조하였다. 그리고 이를 실험군 1 내지 4의 닭에 급여하였다. A feed was prepared by mixing the additive composition of Examples 1 to 4 in 1% by weight of the compound feed for broilers. And it was fed to the chickens of experimental groups 1 to 4.
그리고 대조군 1로서 닭에 일반사료만을 급여하였으며, 대조군 2로서 닭에 일반 육계용 배합사료와 아빌라마이신과 살리노마이신을 1:1 중량비로 혼합한 항생제 200ppm을 혼합한 동물사료를 급여하였다. And as Control 1, only general feed was fed to chickens, and as Control 2, chickens were fed an animal feed mixed with a general broiler compound feed and an antibiotic 200 ppm mixed with avilamycin and salinomycin in a 1:1 weight ratio.
상기 실험군 1 내지 4, 대조군 1, 2는 각각 16주령의 산란계 중종 200마리를 7일간 자유급식량으로 급여하면서 사료 섭취량, 산란율 및 계란의 평균무게를 측정하여 하기 표 2에 나타내었다.The experimental groups 1 to 4 and the control groups 1 and 2 each were fed 200 16-week-old laying hens as a free feed for 7 days while measuring feed intake, egg laying rate and average weight of eggs, and are shown in Table 2 below.
(실시예 1)experimental group
(Example 1)
(실시예 2)experimental group
(Example 2)
(실시예 3)experimental group
(Example 3)
(실시예 4)experimental group
(Example 4)
상기 표 2에서와 같이, 실시예 1 내지 4의 실험군은 대조군 1에 비해 산란율, 계란의 평균 무게가 증가한 것을 확인할 수 있었으며, 대조군 2에 비해 사료 섭취량이 적은 반면, 산란율 및 계란의 평균 무게는 비슷한 수준으로, 사료 효율 역시 개선됨을 확인할 수 있었다. As shown in Table 2, it was confirmed that the experimental groups of Examples 1 to 4 increased the laying rate and the average weight of eggs compared to Control 1, and the feed intake was lower than that of Control 2, whereas the laying rate and the average weight of eggs were similar. level, it was confirmed that the feed efficiency was also improved.
(시험예 3)(Test Example 3)
다보탑 고추를 50일 동안 육묘하고 정식한 후, 상기 실시예 1 내지 4의 첨가제 조성물을 물에 100배 희석하여, 고추 1주당 1L의 관주량으로, 15일 간격으로 관주하여 70주를 재배한 후, 고추의 평균 과수를 비교하였다. 그 결과는 하기 표 3과 같았다.After seedling and planting Dabotap peppers for 50 days, the additive composition of Examples 1 to 4 was diluted 100 times in water, and irrigated at 15-day intervals at an irrigation amount of 1L per pepper per week, after cultivating 70 weeks. , and the average number of red peppers was compared. The results were shown in Table 3 below.
상기 표 3에서와 같이, 실시예 1 내지 4를 시비한 고추는 무처리군에 비하여 그 평균 과수가 현저히 높음을 확인하였다.As shown in Table 3, it was confirmed that the average number of fruits of the peppers fertilized with Examples 1 to 4 was significantly higher than that of the untreated group.
(시험예 4)(Test Example 4)
계분 7kg, 목분 3kg을 혼합하여 부식유기질비료를 제조하였다. 그리고 이에 실시예 1 내지 4의 시료를 0.2kg씩 혼합하고, 최초암모니아 수치를 기준(100%)으로 시간 경과에 따른 암모니아 수치를 측정하여 하기 표 4에 나타내었다. 대조군으로는 가루탈취제(MW101-03) 0.2kg를 사용하였다.A humic organic fertilizer was prepared by mixing 7 kg of chicken manure and 3 kg of wood flour. Then, 0.2 kg of each of the samples of Examples 1 to 4 was mixed, and the ammonia level over time was measured based on the initial ammonia level as a reference (100%), and is shown in Table 4 below. As a control, 0.2 kg of powder deodorant (MW101-03) was used.
상기 표 4에서와 같이, 실시예 1 내지 4는 모두 암모니아 수치가 초기 대비 현저히 저감하여 탈취효과가 우수함을 확인할 수 있었다. 이는 발효작용에 의한 것으로 판단되었다. 특히, 실시예 4는 그 저감효과가 월등한바, 옥수수속대의 발효추출물을 더 포함하는 경우 탈취효과가 현저히 상승함을 확인할 수 있었다.As shown in Table 4, in Examples 1 to 4, the ammonia level was significantly reduced compared to the initial stage, and it was confirmed that the deodorization effect was excellent. It was determined that this was due to fermentation. In particular, it was confirmed that Example 4 had a superior reduction effect, and the deodorization effect was significantly increased when the fermented extract of corncobs was further included.
이상, 본 발명을 바람직한 실시예를 사용하여 상세히 설명하였으나, 본 발명의 범위는 특정 실시예에 한정되는 것은 아니며, 첨부된 특허청구범위에 의하여 해석되어야 할 것이다 또한, 이 기술분야에서 통상의 지식을 습득 한 자라면, 본 발명의 범위에서 벗어나지 않으면서도 많은 수정과 변형이 가능함을 이해하여야 할 것이다.In the above, the present invention has been described in detail using preferred embodiments, but the scope of the present invention is not limited to specific embodiments, and should be interpreted by the appended claims. It should be understood by those skilled in the art that many modifications and variations are possible without departing from the scope of the present invention.
Claims (4)
상기 유용미생물 배양액은 유용미생물(EM) 원액, 당밀 및 물을 혼합하여 배양한 것임을 특징으로 하는 스테비아를 이용한 비료용 첨가제 조성물.
It contains as an active ingredient a fermented product of a mixture of useful microorganism culture broth, stevia, hwangchil extract, goblin fan and haengchae,
The useful microorganism culture solution is an additive composition for fertilizer using stevia, characterized in that it is cultured by mixing an effective microorganism (EM) stock solution, molasses and water.
상기 혼합물은,
상기 유용미생물 배양액 100중량부에 대하여, 상기 스테비아 0.1~100중량부, 황칠 추출물 0.1~100중량부, 도깨비 부채 잎 0.01~50중량부 및 행채 0.01~50중량부를 포함하는 것이고,
상기 스테비아는, 잎, 줄기 중 1종 이상을 분말화한 분말; 및 잎, 줄기 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고,
상기 황칠 추출물은, 황칠나무의 수액; 및 황칠나무의 수피, 줄기 및 잎 중 1종 이상을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하며,
상기 도깨비 부채는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하고,
상기 행채는, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것임을 특징으로 하는 스테비아를 이용한 비료용 첨가제 조성물.
According to claim 1,
The mixture is
With respect to 100 parts by weight of the useful microorganism culture solution, 0.1 to 100 parts by weight of stevia, 0.1 to 100 parts by weight of Hwangchil extract, 0.01 to 50 parts by weight of goblin fan leaves and 0.01 to 50 parts by weight of haengchae,
The stevia is a powder obtained by pulverizing one or more of leaves and stems; and an extract obtained by extracting at least one of leaves and stems using water as an extraction solvent; comprising at least one of
The hwangchil extract, the sap of hwangchil tree; and extracts obtained by extracting at least one of the bark, stems and leaves of Hwangchil tree using water as an extraction solvent; including one or more of
The goblin fan, powdered leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; comprising at least one of
The haengchae is a powder made from a powdered plant; and extracts obtained by extracting whole plants using water as an extraction solvent; An additive composition for fertilizer using stevia, characterized in that it comprises at least one of them.
상기 혼합물은,
분홍애기풀 0.01~50중량부, 카스타놉시스 에키노카르파 0.01~50중량부를 더 포함하고,
상기 분홍애기풀은, 전초를 분말화한 분말; 및 전초를 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것이며,
상기 카스타놉시스 에키노카르파는, 잎을 분말화한 분말; 및 잎을 물을 추출용매로 하여 추출한 추출물; 중 1종 이상을 포함하는 것임을 특징으로 하는 스테비아를 이용한 비료용 첨가제 조성물.
3. The method of claim 2,
The mixture is
It further comprises 0.01 to 50 parts by weight of pink algae, 0.01 to 50 parts by weight of Castanopsis echinocarpa,
The pink rosemary is a powder obtained by pulverizing the outpost; and extracts obtained by extracting whole plants using water as an extraction solvent; It contains one or more of
The Castanopsis echinocarpa is a powder obtained by pulverizing leaves; and extracts obtained by extracting the leaves using water as an extraction solvent; An additive composition for fertilizer using stevia, characterized in that it comprises at least one of them.
상기 유효성분으로 옥수수속대의 발효추출물을 더 포함하되,
상기 옥수수속대의 발효추출물은 옥수수속대에 락토바실러스 람노서스(Lactobacillus rhamnosus)를 접종하여 발효하고, 이를 물을 추출용매로 하여 추출한 추출물인 것을 특징으로 하는 스테비아를 이용한 비료용 첨가제 조성물.According to claim 1,
Further comprising a fermented extract of corncobs as the active ingredient,
The fermented extract of corncobs is an extract obtained by inoculating and fermenting corncobs with Lactobacillus rhamnosus, and using water as an extraction solvent. An additive composition for fertilizer using stevia.
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KR102627671B1 (en) | 2023-02-16 | 2024-01-23 | 주식회사 진설초해 | Composition of high concentration saponin fertilizer including ellow lacquer extract, The fertilizer using the composition, And the cultivation grow using the fertilizer |
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