KR102427458B1 - Freeze-drying methods and apparatus - Google Patents

Abstract

In the freeze-drying method of the present invention, the material is brought into contact with dry ice so that the material has a freezing temperature, and the material is exposed to a vacuum environment to desorb moisture from the material by sublimation of dry ice and vacuum, and at the same time, dehydration by rotation of the material Through the process, the substance to be obtained and the substance to be excluded are separated from the substance.

Description

Freeze-drying methods and apparatus {Freeze-drying methods and apparatus}

The present invention relates to a freeze-drying method and apparatus, and more particularly, by contacting a material with dry ice to have a freezing temperature and dehydrating the material while desorbing moisture from the material through the sublimation phenomenon of dry ice and a vacuum environment. It relates to a freeze-drying method and apparatus for classifying and classifying substances subject to and exclusion.

Adipose tissue metastasis to various parts of the body is a relatively common cosmetic, therapeutic and structural procedure that involves harvesting adipose tissue from one site and re-implantation of harvested and sometimes treated tissue to another site.

Adipose tissue metastasis is mainly used to repair cosmetic defects such as facial folds, wrinkles, smallpox scars and divots, but recently cosmetic and/or therapeutic breast augmentation and reconstruction. (Bircoll and Novack 1987; and Dixon 1988), and hip augmentation (Cardenas-Camarena, Lacouture et al. 1999; de Pedroza 2000; and Peren, Gomez et al. 2000).

In the past, adipose tissue graft and adipose tissue metastasis methods have been used for necrosis, implant resorption by the body, infection (Castello, Barros et al. 1999; Valdatta, Thione et al. 2001), calcification and scarring (Huch, Kunzi et al. 1998), inconsistent engraftment (Eremia and Newman 2000), lack of durability, and other problems resulting from necrosis and lack of neovascularization of the transplanted tissue.

One of the biggest challenges in adipose tissue metastasis is the uptake of the graft by the body after metastasis and the maintenance of the volume of the adipose tissue graft. When adipose tissue is harvested or washed, the spaces between the individual pieces of harvested adipose tissue are filled with a liquid (eg, water, blood, tumescent solution, oil).

When this tissue/fluid mixture is implanted into a recipient, the liquid portion is rapidly absorbed by the body resulting in volume loss. The process of removing an amount of fluid from a tissue/fluid mixture is frequently referred to as adipose tissue drying or adipose tissue dehydration.

In addition, the content of red blood cells, white blood cells, and the like in the adipose tissue graft may also significantly affect the volume of the graft maintained after graft transplantation due to induction or exacerbation of an inflammatory response. Another aspect of tissue maintenance relates to the amount of lipids in the adipose tissue graft. The presence of free lipids (referring to lipids released from dead or damaged adipocytes; also called oil) in adipose tissue grafts may cause or exacerbate inflammatory responses by substantial phagocytic activity and resultant loss of graft volume. understand that you can

It is also known that the mixing of an enriched population of untreated adipose tissue and adipose-derived regenerative cells overcomes many of the problems associated with adipose tissue grafts and adipose tissue metastases as described above.

In particular, replenishing untreated adipose tissue with an enriched population of adipose-derived cells, including adipose-derived stem cells, increases the weight, angiogenesis and maintenance of adipose grafts (U.S. Patent No. 7,390,484 and co-pending U.S. Patent Application Publication Nos. 2005/0025755, which is expressly incorporated herein by reference in its entirety). Adipose tissue fragments supplemented or mixed with an enriched population of cells comprising adipose-derived stem cells show improved angiogenesis and perfusion in the graft compared to the unsupplemented adipose tissue graft alone in an animal model.

Moreover, adipose tissue grafts supplemented with adipose-derived regenerative cells, including adipose-derived stem cells, exhibit increased graft retention and weight over time as compared to unsupplemented grafts (U.S. Patent Application Publication No. 2005/0025755). see). In addition, treatment of adipose tissue in a closed sterile fluid pathway greatly reduces the chance of infection.

In addition, the improvement of adipose tissue autologous metastasis shown in the above animal model was reproduced in human clinical studies. Nevertheless, the isolation and purification of an enriched population of adipose-derived regenerative cells, including adipose-derived stem cells (ADSCs), usually involves a series of washing, digestion, filtration and/or centrifugation steps, which reduce viable cell yields. may reduce and/or require mechanical equipment and specialized clinicians and/or may impair the quality, appearance, longevity, hydration or efficacy of the graft.

Republic of Korea Patent Registration No. 10-1265381 (2013.05.10)

In order to solve the above problems, an object of the present invention is to dehydrate the material to be excluded from the material while desorbing moisture from the material through the sublimation phenomenon of dry ice and a vacuum environment, while the material has a freezing temperature through dry ice. It is intended to be obtained in a freeze-dried state of

Another object of the present invention is to separate and collect the exclusion target substances excluding the target substance from the substance.

Another object of the present invention is to block the moisture desorbed from the material from being transferred to the pressure unit.

Another object of the present invention is to maintain the freezing temperature through a separately installed refrigerator when a temperature decrease of the freezing temperature occurs when the dry ice is exhausted.

In order to achieve the above object, the freeze-drying apparatus of the present invention includes a first chamber containing a substance and dry ice to be brought into contact, a power unit for rotating the first chamber, a second chamber accommodating the first chamber, and the It consists of a pressure part that maintains the internal space of the second chamber in a vacuum state, and dehydrates the substance through the power part while desorbing moisture from the substance by sublimation of dry ice and the vacuum state to separate the substance to be obtained and the substance to be excluded from the substance. let it be

According to the present invention, the first chamber is formed with a plurality of perforations.

According to the present invention, a collecting means is installed in the first chamber to absorb the material to be excluded.

According to the present invention, the second chamber is connected to the pressure part by a pipe.

According to the present invention, a temperature sensor is installed in the second chamber to sense a temperature reduction that occurs when the sublimated dry ice is exhausted, and provides a freezing temperature to the internal space through a refrigerator connected to the second chamber.

According to the present invention, the second chamber further includes a pressure valve, and the pressure valve is configured to discharge the internal space pressure of the second chamber to the outside when the vacuum state of the internal space of the second chamber is changed to overpressure.

According to the present invention, a colt trap is installed between the second chamber and the pressure unit to block moisture transfer to the pressure unit.

According to the present invention, the collecting means is laminated mesh on the front and back.

In addition, in the freeze-drying method of the present invention, the material is exposed to a vacuum environment by contacting the material with dry ice so that the material has a freezing temperature. Through the dehydration process by

In addition, in the freeze-drying method of the present invention, the material and dry ice are pulverized and mixed so that the material and the culture solution have a freezing temperature and exposed to a vacuum environment to desorb moisture from the material by sublimation of dry ice and vacuum, and at the same time, the material Through the dehydration process by rotation of

In addition, the freeze-drying method of the present invention comprises a process of mixing a material and a culture solution and then contacting it with dry ice so that the material and the culture solution have a freezing temperature and exposing them to a vacuum environment to sublimate the dry ice and desorb moisture from the material by vacuum; , at the same time to separate the material to be obtained and the material to be excluded from the material through the dehydration process by rotation of the material.

According to the invention, the material is centrifuged and then brought into contact with dry ice.

According to the present invention, the material is applied by selecting any one of organic material, biomaterial, fat, fat material, ECM, inorganic material, and culture medium, or applying a combination of one or more materials.

According to the present invention, through the dehydration process, the remaining exclusion target materials except for the target material to be obtained are separately collected from the material.

According to the present invention, the target material to be obtained further includes irradiating gamma rays.

The freeze-drying method and apparatus of the present invention as described above include the process of contacting a material with dry ice so that the material has a freezing temperature and exposing it to a vacuum environment to sublimate the dry ice and desorb moisture from the material by vacuum, and at the same time By separating the substance to be obtained and the substance to be excluded from the substance through the dehydration process by rotation of the substance, there is an effect of improving the prevention of spoilage of the substance to be obtained, the shelf life, and the storability.

As a result, there is an effect that can minimize the physical and chemical changes of the obtained metabolites.

In addition, the freeze-drying method and apparatus of the present invention provide convenience for obtaining the material to be obtained by separately collecting the remaining exclusion target materials except for the material to be obtained through the collecting means installed inside the chamber.

In addition, the freeze-drying method and apparatus of the present invention has an effect of preventing damage to the pressure unit by moisture by installing a cold trap between the chamber and the pressure unit to block the transfer of moisture generated in the chamber to the pressure unit.

In the freeze-drying method and apparatus of the present invention, when a temperature sensor is installed in the chamber to sense the temperature reduction state of the internal space that changes when the defrosting is exhausted by sublimation, the freezing temperature is provided to the internal space through the refrigerator connected to the chamber to provide stable freezing It has the effect of providing an environment.

The freeze-drying method and apparatus of the present invention further include a pressure valve in the second chamber, wherein the pressure valve discharges the internal space pressure of the second chamber to the outside when the vacuum state of the internal space of the second chamber is changed to overpressure When the vacuum state of the inner space of the second chamber is not achieved due to a failure of the pressure part, it prevents overpressure in the inner space of the second chamber by continuous sublimation of dry ice to maintain stable vacuum pressure. has the effect of

1 is a block diagram showing the freeze-drying apparatus of the present invention.
Figure 2 is an enlarged cross-sectional view showing the first chamber of the freeze-drying apparatus of the present invention.
Figure 3 is an enlarged cross-sectional view showing another embodiment of the first chamber of the freeze-drying apparatus of the present invention.

Hereinafter, the present invention will be described in detail with reference to the accompanying drawings. First, it should be noted that in the drawings, the same components or parts are denoted by the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted so as not to obscure the gist of the present invention.

As used herein, the terms about, substantially, etc., are used in a sense at or close to the numerical value when the manufacturing and material tolerances inherent in the stated meaning are presented, and are intended to be precise or to aid in the understanding of the present invention. Absolute figures are used to prevent unreasonable exploitation of the stated disclosure by unscrupulous infringers.

Figure 1 is a block diagram showing the freeze-drying apparatus of the present invention, Figure 2 is an enlarged cross-sectional view showing the first chamber of the freeze-drying apparatus of the present invention, Figure 3 is another embodiment of the first chamber of the freeze-drying apparatus of the present invention It is an enlarged cross-sectional view showing an example.

The freeze-drying method of the present invention is as follows.

It is possible to obtain a freeze-dried material to be obtained by separating the material to be obtained and the material to be excluded from the material while desorbing moisture from the material through an environment equipped with a freezing temperature and a vacuum state.

For example, if the substance is fat obtained from a living body, water and free oil are desorbed and dehydrated from the fat so that the freeze-dried fat can be obtained from stem cells.

Here, the material may be applied by selecting any one of organic material, biomaterial, fat, fat material, ECM, inorganic material, and culture medium, or combining one or more materials.

That is, the freeze-drying method has three main examples.

In the first embodiment, the material is brought into contact with dry ice so that the material has a freezing temperature, and the material is exposed to a vacuum environment to sublimate the dry ice and desorb moisture from the material by vacuum, and at the same time, a dehydration process by rotation of the material. It is separated into substances subject to acquisition and substances subject to exclusion.

In the second embodiment, the material and dry ice are pulverized and mixed so that the material and the culture medium have a freezing temperature and exposed to a vacuum environment to sublimate the dry ice and desorb moisture from the material by vacuum, and at the same time, by rotating the material Through the dehydration process, the substance is separated into a substance to be obtained and a substance to be excluded.

The third embodiment is the process of desorbing moisture from the material by sublimation of dry ice and vacuum by exposing it to a vacuum environment while mixing the material and the culture solution and contacting the material with dry ice so that the material and the culture solution have a freezing temperature. Through the spin-drying process, the material is separated into a target substance to be obtained and a substance to be excluded.

In the above embodiment, the material is contacted with dry ice, or the material is ground and mixed with dry ice, or the material and the culture solution are mixed and then contacted with dry ice.

The material has a freezing temperature through dry ice.

And, when the material is exposed to a vacuum environment, moisture is desorbed from the material through the sublimation phenomenon of dry ice.

In addition, when the material is rotated, the material to be obtained and the material to be excluded are separated from the material through a dehydration process, and the material to be excluded is collected separately.

Specifically, the material is brought into contact with dry ice or mixed with dry ice so that the material has its own freezing temperature.

Here, the pulverization of the material pulverizes the particles of the material through a conventional pulverizer.

Here, the reason for pulverizing the material is that the pulverized particles of the dry ice increase the contact surface area with the material, so that the sublimation phenomenon of the dry ice is promoted to have an effective freezing temperature of the material.

Here, dry ice is solid carbon dioxide made by compressing gaseous carbon dioxide, and in addition to the function of imparting a freezing temperature, a material to be obtained extracted from a material to be described later can create an environment for culture through carbon dioxide expressed by sublimation of dry ice.

As described above, when a material having a freezing temperature is placed in a space where the pressure is lowered to below the triple point, the moisture contained in the material is sublimated to a gaseous state and dried.

That is, the moisture contained in the material having a freezing temperature is sublimed under vacuum pressure to desorb moisture.

Freeze-drying is to sublimate and remove moisture from a material, and is a three-dimensional drying method by the intersection of three phases (phase diagram of water) such as sublimation and freezing using thermal properties and vapor pressure of vacuum.

Such freeze-drying has a function of lowering the moisture content per volume of the material through improvement of biochemical properties.

It has a dehydration process by rotating the material simultaneously with the freeze-drying process.

In the dehydration process, the target substances extracted from the substances are separated from the substances subject to exclusion, and then the substances subject to exclusion can be separately collected and removed.

The dehydration process uses centrifugal force generated by rotating the material to separate the material to be obtained and the material to be excluded from the material.

As a result, moisture is desorbed from the material to obtain the material to be obtained in a freeze-dried state in a solidified state, thereby improving the prevention of spoilage of the material to be obtained, shelf life, and storability.

In addition, the solidified material to be obtained may be irradiated with gamma rays.

This is to minimize the transplant rejection reaction in the process of cross-transplantation of the material to be obtained.

In addition, differentiation and proliferation of the target substance can be controlled by irradiation with low-level gamma rays.

1 to 3, the freeze-drying apparatus of the present invention consists of a first chamber 110, a power unit 140, a second chamber 130, and a pressure unit 150.

The first chamber 110 has an inner space 111 having an open upper portion, and a material is filled in the inner space 111 .

Here, a plurality of perforations 112 may be formed on the surface of the first chamber 110 .

Then, a mesh and an absorbent member are laminated on the inner surface of the inner space 111 of the first chamber 110, and then dry ice contained in the mesh body is placed in the inner space.

Then, the material contained in the mesh is placed in contact with the upper surface of the dry ice to transfer the freezing temperature of the dry ice to the material.

Then, the power unit 140 rotates the first chamber 110 to dehydrate the material.

The power unit 140 is composed of a motor or the like, and connects the rotating shaft of the power unit 140 to the lower surface of the first chamber 110 to rotate the first chamber 110 to separate the material to be obtained and the material to be excluded from the material by centrifugal force.

That is, in the first chamber 110, except for the material to be obtained, the remaining materials to be excluded are separated and absorbed in a collecting means to be described later.

The second chamber 130 accommodates the first chamber 110 in the inner space 113 .

In addition, the pressure unit 150 maintains the internal space 113 of the second chamber 130 in a vacuum state.

In other words, the pressure part 150 of the second chamber 130 is connected to the pipe line 132 to generate a vacuum pressure in the inner space 113 of the second chamber 130 .

In the same configuration as above, a freezing temperature is applied through dry ice, and a dehydration process by rotation is performed while desorbing moisture 43 from the material through sublimation of dry ice and a vacuum environment to dehydrate the excluded material from the material. to obtain

Here, the exclusion target material is absorbed by the collecting means 120 .

The collecting means 120 installs the absorbent member 121 to absorb and collect the exclusion target material in the dehydration process.

In particular, the first mesh 122 and the second mesh 123 are stacked on the front and rear surfaces of the absorbent member 121 so as to be closely supported on the inner surface of the first chamber 110 .

That is, the first mesh 122 and the second mesh 123 maintain a state in which the absorbing member 121 is wrapped around the inner surface when the first chamber 110 rotates during the dehydration process.

The second chamber 130 provides a stable freezing environment through the refrigerator 170 connected to the second chamber 130 by installing a temperature sensor 133 to sense a temperature reduction that occurs when the sublimated dry ice is exhausted.

That is, in order to prevent a problem in which the temperature of the internal temperature of the first chamber 110 is reduced in the process of being consumed while the dry ice is sublimed and thus not satisfying the freezing temperature of the material, the inside of the first chamber 110 and the second chamber 120 is If a temperature change occurs above the freezing temperature in the temperature sensor 133 that measures the temperatures of the spaces 111 and 113, the freezing temperature is provided to the internal space 113 of the second chamber 130 through the refrigerator 170 in anticipation of the dry ice exhaustion.

In addition, a colt trap 160 is installed between the second chamber 130 and the pressure unit 150 to block moisture transfer to the pressure unit 150 .

Here, the cold trap 160 is a device for effectively blocking the vacuum pump vacuum load of the pressure unit 150, moisture and various organic solvent gases that cause corrosion and heat load.

In addition, the second chamber 130 further includes a pressure valve 134 .

The pressure valve 134 causes the pressure in the inner space of the second chamber 130 to be discharged to the outside when a problem occurs that the vacuum state of the inner space 131 of the second chamber 130 changes due to overpressure.

That is, when the vacuum state of the inner space 131 of the second chamber 130 is not achieved due to the failure of the pressure unit 150 made of the pump, the overpressure of the inner space 131 of the second chamber 130 is prevented from occurring due to the continuous sublimation of dry ice. This has the effect of maintaining a stable vacuum pressure.

The present invention described above is not limited by the above-described embodiments and the accompanying drawings, and it is common in the technical field to which the present invention pertains that various substitutions, modifications and changes are possible without departing from the technical spirit of the present invention. It will be clear to those who have the knowledge of

100: freeze-drying device
110: first chamber 111: internal space
112: perforated 120: collecting means
121: absorption member 122: first mesh
123: second mesh 130: second chamber
131: interior space 132: pipeline
133: temperature sensor 134: pressure variable
140: power unit 150: pressure unit
160: cold trap 170: freezer

Claims (15)

It has an internal space 111 with an open upper part having a plurality of perforations and is formed to contain the material and dry ice in the internal space 111 so that the material and the dry ice are in contact, but the material contained in the mesh is placed on the upper surface of the dry ice in contact with the dry ice to lower the freezing temperature of the dry ice. A first chamber that is provided with a mesh-type collecting means to absorb the material to be excluded while delivering it to the material;
a power unit for rotating the first chamber;
a second chamber accommodating the first chamber and installing a temperature sensor therein to sense a temperature reduction that occurs when the sublimated dry ice is exhausted and provide a freezing temperature to the internal space through a refrigerator connected to the second chamber;
Consists of a pressure part for maintaining the internal space of the second chamber in a vacuum state,
Contacting a substance with dry ice or grinding a substance with dry ice, or mixing a substance and a culture solution and then contacting the substance with dry ice so that the substance has a freezing temperature and exposing it to a vacuum environment Freeze-drying apparatus, characterized in that the material is separated into a target material to be obtained and a material to be excluded through a dehydration process by rotation of the material at the same time as the process of desorbing the material. delete delete According to claim 1,
The second chamber is a freeze-drying apparatus, characterized in that connected to the pressure unit and a pipe.
delete According to claim 1,
The second chamber further includes a pressure valve, and the pressure valve is configured to discharge the internal space pressure of the second chamber to the outside when the vacuum state of the internal space of the second chamber is changed to overpressure. . According to claim 1,
Freeze-drying apparatus, characterized in that by installing a colt trap between the second chamber and the pressure part to block the transfer of moisture to the pressure part. delete delete delete delete delete delete delete delete

Images