KR102375826B1 - Amino acid mineral complex for supplement magnesium and food or feed compositon comprising it - Google Patents

Abstract

The present invention relates to an amino acid mineral complex for supplementing magnesium and a composition for food or feed comprising the same, and more particularly, to a human or an animal other than a human being supplemented with magnesium using the amino acid mineral complex as an active ingredient due to magnesium deficiency. It relates to a health functional food, feed (or feed additive) composition that prevents diseases and enables energy metabolism and body function recovery, protein synthesis, and maintenance of nerve and muscle functions.

Description

Amino acid mineral complex for supplementation of magnesium and composition for food or feed comprising the same

The present invention relates to an amino acid-mineral complex for supplementing magnesium and a composition for a health functional food or feed comprising the same.

The advantage of amino acid-mineral complexes in mineral nutrition is the fact that they are readily absorbed by active transport or other mechanisms in mucosal and plant cells. That is, when minerals are absorbed using amino acids as transport molecules, there is an advantage in that it is possible to avoid problems related to competition for the active site for absorption and the function of inhibiting absorption of special trace elements between minerals.

A mineral amino acid complex is generally produced by a reaction between an alpha-amino acid and a metal ion, and in order for the complex to have a ring structure, a metal ion having a valence of 2 or more is required. In this reaction, the positive charge of the metal ion is neutralized by reacting with the negative charge of the amino or carboxyl group of the alpha-amino acid.

The structure and chemical formula of the mineral amino acid complex and the related literature on bioavailability are very diverse, representatively, Ashmead et al., Chelated Mineral Nutrition, (1982), Chas. C. Thomas Publishers, Springfield, Ill. ), Ashmead et al. (Intestinal Absorption of Metal Ions, (1985)), Ashmead et al. (Ashmead et al., Foliar Feeding of Plants with Amino Acid Chelates, (1986)) and U.S. Patent No. 4,020,158 , 4,167,564, 4,216,143, 4,721,644, 4,599,152, 4,774,089, 4,830,716, 4,863,898, 4,725,427, and the like.

U.S. Patent No. 4,020,158 U.S. Patent No. 4,167,564

Ashmead et al., Chelated Mineral Nutrition, (1982), Chas. C. Thomas Publishers, Springfield, Ill. Chas. C. Thomas Publishers, Springfield, Ill. Ashmead et al., Intestinal Absorption of Metal Ions, (1985) Ashmead et al., Foliar Feeding of Plants with Amino Acid Chelates, (1986)

The present invention is to provide an amino acid mineral complex having a novel chemical formula.

Another object of the present invention is to provide a method for preparing the amino acid-mineral complex.

Another object of the present invention is to provide a composition for replenishing magnesium comprising the amino acid-mineral complex.

The amino acid-mineral complex of the present invention for achieving the above object may have a molecular formula C ₄ H ₁₃ MgNO ₈ and may be magnesium aspartic acid hydrate represented by the following formula (1):

[Formula 1]

.

.

The magnesium aspartic acid hydrate may be water soluble.

The amino acid mineral complex may be for magnesium supplementation.

In addition, the method for preparing the amino acid-mineral complex of the present invention for achieving the above other object includes the steps of: adding a magnesium precursor and aspartic acid to water in a molar ratio of 1: 0.8 to 2.5; and heating the magnesium precursor and aspartic acid mixed aqueous solution of the above step at 50 to 100° C. for 10 minutes to 24 hours to react; may include.

In addition, the feed composition for supplementing magnesium of the present invention for achieving the above another object may contain the amino acid-mineral complex as an active ingredient.

In addition, the dietary supplement composition for magnesium of the present invention for achieving the above another object may contain the amino acid mineral complex as an active ingredient.

The daily intake of the amino acid mineral complex may be 66-250 mg.

The health functional food composition may be capable of energy metabolism and recovery of body functions, protein synthesis, and maintenance of nerve and muscle functions.

The amino acid-mineral complex of the present invention is water-soluble and can be quickly dissolved in water even when formulated into tablets such as powders, granules, and effervescent tablets.

In addition, the composition for magnesium absorption using the amino acid-mineral complex of the present invention as an active ingredient can provide magnesium as well as increase the absorption rate of the provided magnesium when applied to humans or animals other than humans, thereby recovering energy metabolism and body function , protein synthesis, and maintenance of nerve and muscle function.

The present invention relates to an amino acid-mineral complex for supplementing magnesium and a composition for a health functional food or feed comprising the same.

Specifically, by providing the amino acid-mineral complex (aminomagnesium) of the present invention to humans or non-human animals to increase the absorption rate of magnesium, diseases that may occur due to magnesium deficiency are prevented, energy metabolism and body function recovery, and protein synthesis , to maintain nerve and muscle function.

Hereinafter, the present invention will be described in detail.

The present invention relates to an amino acid-mineral complex having a molecular formula C ₄ H ₁₃ MgNO ₈ and magnesium aspartic acid hydrate represented by the following formula (1).

[Formula 1]

The magnesium aspartic acid hydrate may be water soluble. The water solubility means that 1 g of the magnesium aspartic acid hydrate is dissolved in 1 to 30 ml of water at 25°C, preferably 1 to 15 ml.

The amino acid mineral complex may be for magnesium supplementation.

The water-soluble amino acid-mineral complex is prepared by adding a magnesium precursor and aspartic acid to water in a molar ratio of 1:0.8 to 2.5; and reacting the magnesium precursor and the mixed aqueous solution of aspartic acid in the above step by heating at 50 to 100° C. for 10 minutes to 24 hours.

The present invention relates to a magnesium supplement composition comprising the amino acid-mineral complex as an active ingredient.

The magnesium precursor is a magnesium salt or magnesium oxide that can be used as food or feed, preferably magnesium oxide, magnesium sulfate, magnesium chloride, or magnesium nitrate, and more preferably magnesium oxide that can increase magnesium absorption. can be heard In particular, it was natural to use a water-soluble magnesium salt that dissolves well in water in the production of the conventional magnesium aspartic acid complex, but a magnesium aspartic acid complex, which is more effective when heated over a certain temperature and time while using water-insoluble magnesium oxide, is produced. formation was confirmed.

The molar ratio of the magnesium precursor and aspartic acid means a molar ratio of magnesium and aspartic acid, and a molar ratio of 1:0.8 to 2.5, preferably 1:1.5 to 2.2 is advantageous. Although the theoretical molar ratio of magnesium and aspartic acid in the magnesium aspartic acid hydrate of Formula 1 is 1:2, when aspartic acid is added in excess compared to the magnesium precursor, insoluble magnesium oxide is reduced and water-soluble magnesium aspartic acid hydrate is more easily formed. can do.

The reacting step is performed at 50 to 100 ° C. for 10 minutes to 24 hours, particularly in the case of a water-soluble magnesium salt, even in the above range, at a relatively low temperature and for a short time, for example, only heating at 50 to 80 ° C. for 10 minutes to 1 hour. In the case of magnesium oxide, it is preferable to heat at 80 to 100° C. for at least 1 hour, preferably for 1.5 hours. On the other hand, since most of the water-soluble magnesium salt or magnesium oxide reacts with about 95%, preferably 99% or more of aspartic acid within 3 hours, the reduction of insolubles or unreacted substances may not be significant even when heated for more than 3 hours. In the reacting step, ultrasonic treatment may be used to shorten the reaction time.

It may further include the step of removing insolubles from the reaction solution of the above step. The step of removing the insoluble material may be added even when a water-soluble magnesium salt is used as a magnesium precursor, but may be particularly required when magnesium oxide is used as a magnesium precursor.

In the step of removing the insoluble matter, the insoluble matter may be filtered with a number of μm or less, for example, 0.2 μm filter paper or filter cloth, and the insoluble matter may be removed before the filtration or through centrifugation separately from the filtration.

The manufacturing method does not require a separate additive other than the magnesium precursor, aspartic acid, and water, unlike the conventional method for manufacturing the magnesium aspartic acid complex, and has the advantage that the manufacturing process is simple and the process cost is reduced.

On the other hand, in the present specification, "with an active ingredient" means to include an amount sufficient to achieve the efficacy or activity of the mineral amino acid complex of the present invention. In one embodiment of the present invention, in the composition of the present invention, the food composition or feed composition has a mineral amino acid complex of, for example, 0.001 mg/kg or more, preferably 0.1 mg/kg or more, more preferably 10 mg/kg or more. Above, more preferably 60 mg/kg or more, even more preferably 150 mg/kg or more, and may be used purely only as a mineral amino acid complex. At this time, it is most preferable that the daily intake of the amino acid mineral complex is 66-250 mg.

The food composition or food additive composition according to the present invention may be formulated in the same manner as a conventional pharmaceutical composition and used as a functional food or added to various foods. Foods to which the food additive composition can be added include, for example, beverages, alcoholic beverages, confectionery, diet bar, dairy product, meat, chocolate, pizza, ramen, other noodles, gum, ice cream, vitamin complex, health supplement Foods, etc.

The food composition of the present invention may include a mineral amino acid complex as an active ingredient, as well as ingredients commonly added during food production, for example, proteins, carbohydrates, fats, nutrients, seasonings and flavoring agents. . Examples of the above-mentioned carbohydrates include monosaccharides such as glucose, fructose and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides, for example, conventional sugars such as dextrin, cyclodextrin, and the like, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [taumatine, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.)) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is prepared as a drink or beverage, citric acid, high fructose, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be additionally included in addition to the amino acid and mineral complex of the present invention. there is.

The present invention provides a health functional food comprising a food composition for supplementing magnesium comprising the mineral amino acid complex as an active ingredient. Health functional food is a food prepared by adding amino acid and mineral complex to food materials such as beverages, teas, spices, gum, and confectionery, or by encapsulating, powdering, or suspension, etc. However, unlike general drugs, it has the advantage of not having side effects that may occur when taking the drug for a long time using food as a raw material. The health functional food of the present invention obtained in this way is very useful because it can be ingested on a daily basis. The amount of amino acid-mineral complex added in such a health functional food varies depending on the type of health functional food and cannot be defined uniformly, but it can be added in a range that does not impair the original taste of the food. -250 mg/day. In addition, in the case of a health functional food in the form of pills, granules, tablets or capsules, it may be added in the range of 66-250 mg/day. In one embodiment, the health functional food of the present invention may be in the form of pills, tablets, capsules or beverages.

In the feed additive composition according to the present invention, the feed additive composition is added and mixed in an amount of 0.01-1% by weight based on the solid content of the feed composition of livestock such as pigs, chickens, ducks, cattle, sheep, goats, and dogs, and mixed with various animals. It can be applied by administration, and more preferably used as an additive in pig feed.

The feed additive composition of the present invention may be prepared as a feed composition in the form of fermented feed, compound feed, pellet form, silage, etc., but is not limited thereto, and may be prepared by modifying it as necessary.

Hereinafter, preferred examples are presented to help the understanding of the present invention, but the following examples are merely illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and spirit of the present invention, It goes without saying that such variations and modifications fall within the scope of the appended claims.

Example 1. Preparation of magnesium aspartic acid complex (using magnesium oxide)

Put 60 g of aspartic acid in 500 ml of water and stir while adding magnesium oxide (magnesium 60.32 wt%) to it and reacting it sufficiently at a temperature of 60 ° C. for at least 30 minutes while stirring, centrifugation to freeze-dry the supernatant, 70 g of a water-soluble magnesium aspartic acid complex was obtained. However, the magnesium oxide and the aspartic acid were added in a content of 1:2 mole ratio.

<Test Example>

Test Example 1. Magnesium Confirmation of the structure of the aspartic acid complex

The structure of the magnesium aspartic acid complex of Example 1 was confirmed using Bruker SMART APEX II X-ray Crystallograph (XRC) and Bruker SHELXTL Structure Analysis Program.

crystallographic data

Molecular formula C ₄ H ₁₃ MgNO ₈

molecular weight 227.46

crystal system orthorhombic system

Space group P2(1)2(1)2(1)

Unit cell dimension a=6.3016(18)Å α=90°

b=10.971(3)Å β=90°

c=13.521(4)Å γ=90°

Volume 934.8(5) / Å ³

Calculated Density 1.616 Mg/m ³

Crystal size 0.18 x 0.08 x 0.02 mm ³

F(000) 480

Table 1 shows atomic coordinates (x10 ⁴ ) and equivalent isotropic displacement parameters (Å ² x10 ³ ). U(eq) is defined as 1/3 of the trace of an orthogonalized Uij tensor.

Table 2 shows the bond length (Å) and angle (°).

Symmetric transformations were used to create equivalent atoms. #1 -x, y-1/2, -z+3/2; #2 -x, y+1/2, -z+3/2.

Table 3 shows the anisotropic displacement parameters (Å ² x10 ³ ). The exponent of the anisotropic displacement factor is in the form of -2π ² [h ² a ^*2 U ¹¹ +...+2hka ^* b ^* U ¹² ].

Table 4 shows hydrogen coordinates (x10 ⁴ ) and equivalent isotropic displacement parameters (Å ² x10 ³ ).

Table 5 shows the torsion angles (°).

Combining the above results, the water-soluble magnesium aspartic acid complex of Example 1 had the molecular formula C ₄ H ₁₃ MgNO ₈ and was confirmed as magnesium aspartic acid hydrate represented by the following formula (1).

[Formula 1]

The structure in which hydrogen is omitted in Formula 1 is shown in Formula 2 below.

[Formula 2]

In the magnesium aspartate hydrate of Formula 1, four water molecules are coordinated to magnesium, and one oxygen atom is coordinated from one hydroxyl group and one nitrogen atom from one amino group is coordinated to have a total of six coordination bonds. It was identified as a novel magnesium aspartic acid hydrate in which magnesium and aspartic acid were combined in a 1:1 molar ratio.

The magnesium aspartic acid hydrate of Formula 1 may coordinate with the aspartic acid of the adjacent magnesium aspartic acid hydrate.

Test Example 2. Measurement of Magnesium Absorption Rate

150-200 g of 4-week-old female SD mice (Orient Bio Inc., Seoul, Korea) were used in the experiment. Before the experiment, the mice were acclimatized for 7 days before being used in the experiment, and during the acclimatization process, they were bred on a free diet in a breeding room with a temperature of 23±1° C. and 60±5% humidity, and the light-dark cycle was controlled at a 12-hour cycle.

The mice are divided into 3 mice each, start feeding the mineral-restricted diet from the 4th day after starting the diet, and on the 26th and 27th (22nd and 23rd days after the mineral-restricted diet) magnesium oxide or Example 1 The amino acid mineral complex was orally administered (2 times in total). Control 1 is a magnesium-deficient group by a mineral-restricted diet, Control 2 is a group administered with magnesium oxide, and Example 1 administration group is a group administered with the amino acid-mineral complex of Example 1.

On the 26th day (the 22nd day after the mineral-restricted diet), feces were collected after oral administration of magnesium oxide or the amino acid-mineral complex of Example 1, and on the 27th day (the 23rd day after the mineral-restricted diet), magnesium oxide or Example After oral administration of the amino acid-mineral complex of 1, it was dissected and serum was collected.

division Feces (mg/g) Serum (mg/dl) Control 1 (mineral depletion) 45.71±4.25 1.20±0.09 Control 2 (magnesium oxide) 255.88±28.98 1.87±0.07 Example 1 (amino acid mineral complex) 90.96±25.15 2.20±0.12

As shown in Table 6 above, it was confirmed that the group administered with the amino acid-mineral complex prepared according to Example 1 of the present invention had a higher magnesium content in feces and serum compared to Controls 1 and 2.

This means that when the amino acid-mineral complex of Example 1 is used in humans or non-human animals, it can provide magnesium as well as increase the absorption rate of the provided magnesium.

Claims (8)

It has the molecular formula C ₄ H ₁₃ MgNO ₈ , is magnesium aspartic acid hydrate represented by the following formula (1), and is orthorhombic crystals, and 1 g of the magnesium aspartic acid hydrate has water solubility in 1 to 30 ml of water at 25° C. Amino Acid Mineral Complex:
[Formula 1]

. delete The amino acid-mineral complex according to claim 1, characterized in that it is for magnesium supplementation. adding the magnesium precursor and aspartic acid to water in a molar ratio of 1:0.8 to 2.5; and reacting the magnesium precursor and the mixed aqueous solution of _aspartic acid at ₅₀ to 100° C. for ₁₀ minutes to 24 hours to react; obtain,
wherein the magnesium aspartic acid hydrate is orthorhombic crystals, and 1 g of the magnesium aspartic acid hydrate has water solubility in 1 to 30 ml of water at 25°C;
[Formula 1]

A feed composition for supplementing magnesium comprising the amino acid-mineral complex of claim 1 or 3 as an active ingredient. A health functional food composition comprising the amino acid-mineral complex of claim 1 or 3 as an active ingredient. The dietary supplement composition for magnesium supplementation, characterized in that the daily intake of the amino acid-mineral complex of claim 6 is 66-250 mg. The health functional food composition of claim 6 is a health functional food composition, characterized in that it is possible to maintain energy metabolism and body function recovery, protein synthesis, and nerve and muscle functions.