KR102374903B1 - Method for preparing ginseng extract with enhanced ginsenoside Rg3 - Google Patents

Abstract

The present invention relates to a method for producing a ginseng extract with enhanced ginsenoside Rg3 content, and more specifically, Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain having saponin bioconversion ability. It relates to a method for producing a ginseng extract capable of increasing the ginsenoside Rg3 component, which is known to have effects such as vasodilation and anticancer action.

Description

Method for preparing ginseng extract with enhanced ginsenoside Rg3 content {Method for preparing ginseng extract with enhanced ginsenoside Rg3}

The present invention relates to a method for producing a ginseng extract with enhanced ginsenoside Rg3 content, and more specifically, to a saponin-in blood vessel using a Vogesella indigofera WSW02 (Accession No. KCCM12637P) strain having saponin bioconversion ability. It relates to a method for producing a ginseng extract capable of increasing the ginsenoside Rg3 component, which is known to have effects such as relaxation and anticancer effects.

Ginseng is a plant belonging to the genus Panax, and the root is used for medicinal purposes. The efficacy of ginseng is due to ginsenoside, an active ingredient present in ginseng.

Ginsenoside is according to the structure of the ginsenoside aglycone, protopanaxadiol-type ginsenoside, protopanaxadiol-type ginsenoside, Protopanaxatriol-type ginsenoside, and olea It can be classified into three types of egg ginsenoside (Oleanane-type ginsenoside).

Ginsenoside derivatives include glucose, rhamnose, xylose, and arabinose in the alcoholic OH groups of R1, R2, and R3 of triterpene, a non-saccharide (protopanaxadiol or protopanaxatriol). ) is a compound having a structure in which saccharides such as saccharides are ester-bonded, and the major ginsenosides of ginseng currently known are about 13 common ginsenosides and about 11 rare ginsenosides known to be converted therefrom. is known as

Ginseng may be used by using fresh ginseng as it is, by drying it to prepare it in the form of white ginseng, or by steaming it to prepare and use it in the form of red ginseng.

Among ginseng, fresh ginseng contains glycoside ginsenosides such as Rg1, Re, Rb1, Rb2, Rc, and Rd. In the case of processed ginseng, rare ginsenosides Rg3, Rh1, Rh2, Compound K It is known that the content of

Recently, many studies have been actively conducted to further increase the content of ginseng saponin present only in processed ginseng. Red ginseng, a representative processed ginseng, can be prepared by steaming fresh ginseng, and in this process, the linkage of glycosides bound to the C-20 position of chemically structurally unstable ginsenoside aglycone may be hydrolyzed. Red ginseng processed through this process contains more ginsenosides Rh1, Rh2, Rg2, Rg3 than fresh ginseng or white ginseng. However, the content of ginsenoside Rg3, a kind of rare ginsenoside, is not high even in red ginseng, and it is complicated to process into red ginseng, causing inconvenience in mass production.

Therefore, in order to supplement the above-mentioned problems, the present inventors recognized that it is urgent to develop a method capable of enhancing the ginsenoside Rg3 content without processing ginseng, and completed the present invention.

Republic of Korea Patent Publication No. 10-1493158 Republic of Korea Patent Publication No. 10-1098467

Chem. Pharm. Bull. 46, 647~654 (1998) Chem. Pharm. Bull. 49,1452~1456 (2001)

An object of the present invention is to use the saponin bioconversion ability of Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain of saponin ginsenoside Rg3 component known to have effects such as vasodilatory action and anticancer action To provide a method for preparing a ginseng extract that can increase

Another object of the present invention is to produce a ginseng extract capable of increasing the ginsenoside Rg3 component effective for immune enhancement without significant destruction of the ginseng saponin component by omitting the step of processing into fermented red ginseng without requiring special facilities or equipment to provide a way to

The technical problems to be achieved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by those of ordinary skill in the art from the description of the present invention.

In order to achieve the above object, the present invention provides a method for preparing a ginseng extract with enhanced ginsenoside Rg3 content.

Hereinafter, the present specification will be described in more detail.

The present invention provides a method for producing a ginseng extract with enhanced ginsenoside Rg3 content, comprising the following steps.

(S1) pulverizing ginseng;

(S3) inoculating the pulverized ginseng with Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain; and

(S3) preparing an extract using a solvent of ginseng contacted with the strain.

In the present invention, the Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain is characterized in that it is inoculated with 3 to 10% by weight compared to the total weight% of the ginseng.

In the present invention, the step (S3) is characterized in that it includes the following steps.

(S3A) adding a solvent to the ginseng contacted with the strain; and

(S3B) Concentrating the solvent-added ginseng at 60 to 75 °C to prepare an extract.

In the present invention, the concentration is characterized in that 40 to 55% of the final concentration% of the ginseng to which the solvent is added by the step (S3A) is concentrated.

In the present invention, the method for preparing the ginseng extract fortified in the ginsenoside Rg3 content includes (S4) filtering the extract prepared in the step (S3) and then freeze-drying or vacuum drying; characterized.

The method for producing a ginseng extract with enhanced ginsenoside Rg3 content of the present invention uses a strain of Vo gesella indigofera WSW02 (Accession No. KCCM12637P) having saponin bioconversion ability to relax blood vessels and have anticancer effects among saponins. It is possible to increase the ginsenoside Rg3 component known to have such effects.

In addition, the method for producing a ginseng extract with enhanced ginsenoside Rg3 content of the present invention can prevent the problem of cross-contamination with other strains because only the selected strains are purely cultured.

In addition, the method for producing ginseng extract with enhanced ginsenoside Rg3 content of the present invention does not require special facilities or equipment, and omits the step of processing into fermented red ginseng, which is effective in enhancing immunity without significantly destroying ginseng saponin components. It can increase the senoside Rg3 component.

Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

1 is data analyzed for homology with strains registered in GenBank by analyzing the 16S rRNA gene sequence of the Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain.
Figure 2 is a test confirmation for measuring the component content of the ginseng extract prepared according to Example 1 of the present invention.

The terms used in this specification have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

Unless defined otherwise, all terms used herein, including technical and scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

Numerical ranges are inclusive of the values defined in that range. Every maximum numerical limitation given throughout this specification includes all lower numerical limitations as if the lower numerical limitation were expressly written. Every minimum numerical limitation given throughout this specification includes all higher numerical limitations as if the higher numerical limitation were expressly written. Any numerical limitation given throughout this specification shall include all numerical ranges within the broader numerical range, as if the narrower numerical limitation were expressly written down.

Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited by the following examples.

Manufacturing method of ginseng extract with enhanced ginsenoside Rg3 content

The present invention provides a method for preparing a ginseng extract with enhanced ginsenoside Rg3 content, comprising the following steps.

(S1) pulverizing ginseng;

(S2) inoculating the pulverized ginseng with Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain; and

(S3) preparing an extract using a solvent of ginseng contacted with the strain.

The step (S1) may be a step of pulverizing ginseng to an appropriate size.

The ginseng may use a variety of known ginseng, and may be one or more selected from the group consisting of Korean ginseng, hoegi ginseng, whole ginseng, bamboo jeol ginseng, samyeop ginseng, himalayan ginseng, western ginseng and Vietnamese ginseng, but is not limited thereto.

The pulverization may pulverize the ginseng to 20 to 60 mesh, preferably pulverize the ginseng to 30 to 60 mesh.

The step (S2) may be a step of inoculating the pulverized ginseng with a strain.

For the preparation of ginseng extract with enhanced ginsenoside Rg3 content according to the present invention, the deposited Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain having saponin bioconversion ability is received and used by culturing. In addition, the Pycnoporus Bogesera indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain was named [WSW02].

The Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) The strain will be described as follows.

The Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain can be used after culturing at 26 ° C., shaking or stationary (liquid, solid) under aerobic or anaerobic conditions.

The Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain may be inoculated with 3 to 10% by weight relative to the total weight% of the ginseng.

The Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) When the strain is inoculated, purified water or distilled water may be mixed and added.

The purified water or distilled water may be added in an amount of 1 to 10% by weight based on the total weight% of the ginseng.

The step (S3) is a step of preparing an extract, and may include the following steps.

(S3A) adding a solvent to the ginseng inoculated with the strain; and

(S3B) Concentrating the solvent-added ginseng at 60 to 75 °C to prepare an extract.

The solvent may be water, a C1-C4 alcohol, and a mixture of water and C1-C4 alcohol, preferably water, ethanol, and a mixed solution of water and ethanol, and most preferably water or ethanol. .

The concentration may be concentrated to 40 to 55% of the final concentration% of the solvent-added ginseng prepared in step (S3A).

In addition, the concentration may be performed for 10 to 15 days, and is not limited thereto, as long as it can be concentrated up to 40 to 55% of the final concentration% of ginseng to which the solvent is added.

The method for preparing a ginseng extract with enhanced ginsenoside Rg3 content may additionally include the following (S4) step.

(S4) After filtering the extract prepared in step (S3), freeze-drying or vacuum-drying.

The filtration may be filtered using a filtration network of 100 to 150 mesh, preferably filtered using a filtration network of 100 to 140 mesh, and most preferably filtered using a filtration network of 110 to 130 mesh. connect.

The freeze-drying or vacuum drying may be performed at a temperature range not exceeding 60 °C.

The method for producing a ginseng extract of the present invention is known to have effects such as vasoconstriction and anticancer action among saponins using a Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain having saponin bioconversion ability. It can increase the ginsenoside Rg3 component.

Advantages and features of the present invention, and methods for achieving them, will become apparent with reference to the embodiments described below in detail. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and only the embodiments allow the disclosure of the present invention to be complete, and common knowledge in the technical field to which the present invention pertains It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

Example 1. Preparation of ginseng extract with enhanced ginsenoside Rg3 content

First, ginseng (Korean ginseng) was ground to a size of 30 mesh, and the pulverized ginseng was inoculated with Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain. At this time, the strain was inoculated at 5% by weight relative to the total weight% of the ginseng, and the Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain was inoculated with distilled water at 3% by weight compared to the total weight% of the ginseng was added with Next, distilled water was added to the ginseng inoculated with the strain and concentrated to 50% of the final concentration of ginseng with the solvent added at 70° C. for 12 days to prepare an extract. Finally, the extract was filtered using a 120 mesh filtration network, and freeze-dried to prepare a ginseng extract according to the present invention.

Comparative Example 1. Preparation of Comparative Ginseng Extract

Four-year-old ginseng (Korean ginseng) was ground to a size of 30 mesh, and added to the pulverized ginseng in an amount of 3 wt% based on the total weight of ginseng. Next, distilled water was added to the ginseng and concentrated to 50% of the final concentration of ginseng to which the solvent was added at 70° C. for 12 days to prepare an extract. Finally, the extract was filtered using a 120 mesh filtration network, and freeze-dried to prepare a comparative ginseng extract.

Experimental Example 1. Confirmation of extract component content

In order to confirm the content of the constituents contained in the ginseng extract prepared according to the present invention, it was requested to measure the constituent content to the Korea Food Research Institute (KFRI), and the results are shown in FIG. 2 and [Table 1] below. indicated. In addition, for comparison with the present invention, the constituent content of the extract prepared in Comparative Example 1 was measured in the same manner, and it is shown in [Table 2] below.

[Table 1]

[Table 2]

2 and [Table 1], it can be seen that the ginseng extract according to the present invention contains a large amount of ginsenoside, and in particular, it can be confirmed that the ginsenoside Rg3 contains a total of 10.85 mg/g. there is. On the other hand, in the case of a comparative ginseng extract that is not inoculated with Vo gesella indigofera WSW02 (Accession No. KCCM12637P) strain according to the present invention, the content of ginsenosides present in the extract is also small, and Rg3 is confirmed in the extract can confirm that it is not.

From the above results, the ginseng extract prepared by the manufacturing method according to the present invention is an extract in which the ginsenoside Rg3 component is fortified using the Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain having bioconversion ability. It can be confirmed that the manufacture of

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be implemented in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive.

Claims (6)

Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain. (S1) pulverizing ginseng;
(S2) inoculating the pulverized ginseng with Bo gesella indigofera WSW02 (Accession No. KCCM12637P) strain; and
(S3) preparing an extract using the ginseng inoculated with the strain as a solvent; ginsenoside Rg3 content of ginseng extract enhanced, characterized in that it comprises a manufacturing method. 3. The method of claim 2,
The Bo gesella indigofera (Vo gesella indigofera ) WSW02 (Accession No. KCCM12637P) strain is a method of producing a ginseng extract with enhanced ginsenoside Rg3 content, characterized in that the strain is inoculated at 3 to 10% by weight relative to the total weight% of the ginseng . 3. The method of claim 2,
The step (S3) is
(S3A) adding a solvent to the ginseng contacted with the strain; and
(S3B) Concentrating the solvent-added ginseng at 60 to 75 ° C. to prepare an extract; A method for producing a ginseng extract with enhanced ginsenoside Rg3 content, comprising: 5. The method of claim 4,
The concentration is 40 to 55% of the final concentration% of the solvent-added ginseng by the step (S3A). 3. The method of claim 2,
The manufacturing method of the ginseng extract with enhanced ginsenoside Rg3 content is
(S4) After filtering the extract prepared in the step (S3), freeze-drying or vacuum-drying; A method for producing a ginseng extract with enhanced ginsenoside Rg3 content, characterized in that it additionally comprises.

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