KR102328824B1 - Composition for Hair Growth Stimulation or Hair Loss Prevention Using Solubilized Mastic Gum and an Extract of Lindera glauca - Google Patents

Abstract

The present invention discloses a composition for promoting hair growth or inhibiting hair loss using a water-soluble mastic gum and an extract of Lindera glauca which promote the proliferation of dermal papilla cells and suppress the expression of 5α-reductase. As described above, according to the present invention, it is possible to provide the composition for promoting hair growth or inhibiting hair loss using the water-soluble mastic gum and the extract of Lindera glauca.

Description

Composition for Hair Growth Stimulation or Hair Loss Prevention Using Solubilized Mastic Gum and an Extract of Lindera glauca

The present invention relates to a composition for promoting hair growth or inhibiting hair loss using water- soluble mastic gum and an extract of Lindera glauca.

Hair is formed from hair follicles, and there are about 5 million hair follicles in adults, which are distributed throughout the body except for palms and sea cucumbers among human skin, and 1 million are present on the head, of which 100,000 are on the scalp. (Bulletin of Food Technology 2009, 22(4):651-663). Scalp hair accounts for about 20% of human hair. It has physiological functions such as sweat evaporation and body temperature control, as well as a function to protect the head from shock by buffering the head. Therefore, it has important social functions for individual individuality and image production (Clin Dermatol 2001, 19(2):161-166).

The exact cause of hair loss has not been fully elucidated so far, but it is caused by inhibition of proliferation or decreased function of dermal papilla cells related to hair cycle regulation, abnormal hair cycle due to the action of male hormones, and decreased blood flow to the scalp. Abnormal changes in the hair cycle caused by this, drugs such as anticancer drugs, mental stress, physical stimulation, and environmental pollution are being discussed (J Invest Dermatol, 1999, 113: 873-877; Mol Cell Endocrinol, 2002 198:89-95; J (Dermatol. Sci. 2011, 62: 154-159).

The dermal papilla cells, a type of dermal fibroblast specialized in the hair bulb, interact with various types of epithelial cells in the hair follicle and play an important role in the formation of hair follicles, hair regeneration, and hair growth. As is known, the growth and proliferation of dermal papilla cells, and inhibition of apoptosis (apoptosis) is an important target for the development of drugs that maintain the growth phase of hair and inhibit hair loss (Physiol. Rev. 81: 449-494; J. Dermatol) (Sci. 2011, 62: 154-159; FASEB J. 2008, 22: 1725-1736).

Cell proliferation is closely related to the progression of the cell cycle, which consists of G0/G1, S and G2/M phases, and this cell cycle progression is the result of the cyclin E/CDK2 complex. It is promoted by activation, cyclin D1 (cyclin D1) increases, increased phosphorylation of pRB, p27 ^KIP1 reduced (Mar. Drugs 2013, 11: 1783-1799 ).

In particular, among these cell cycle proteins, cyclin D1 is known as one of the target genes of Wnt/β-catenin signaling as a protein that increases when the cell cycle progresses from G0/G to S phase. (Johnson et al., 1999; Sherr 1996; Prall et al., 1997), Wnt/β-catenin signaling also plays an important role in hair growth and hair regeneration. Wnt/β-catenin signaling is reported to be regulated by the actions of GSK3β (glycogen synthase kinase-3β), PKA (protein kinase A), Akt, etc. (Dev. Biol. 2005, 185: 82-91; Mol. Cell. Biol. 2005, 25: 9063-9072; J. Immunol. 2001, 166: 4713-4720).

Currently, two types of drugs that promote hair growth have been approved by the Food and Drug Administration (FDA): minoxidil and finasteride. Minoxidil was initially developed as a vasodilator for the treatment of hypertension, but was developed as a hair growth agent after reports of hirsutism as a side effect. K _ATP channel (ATP-sensitive K ⁺ -channel) opening effect, activation of Wnt/β-catenin signaling, and dermal papilla cell apoptosis inhibitory effect are thought to induce hair growth ( J Dermatol Sci. 2004, 34: 91-98). In addition, finasteride developed by Merck is a substance that inhibits the activity of 5α-reductase, an enzyme that acts on male hormone metabolism, and was developed as a treatment for enlarged prostate. It is a known and approved drug for hair growth (Mol Cell Endocrinol, 1999, 198:89-95). Originally, 5α-reductase is an enzyme that converts the male hormone testosterone into dihydrotestosterone (DHT). Androgen receptor (androgen receptor) that binds to (agonist) is also reduced, ultimately preventing hair loss symptoms.

Because hair loss treatment requires long-term treatment, it is important to minimize side effects. Accordingly, efforts are being made to discover new hair loss treatment materials from natural products with relatively few side effects to the human body.

It is an object of the present invention to provide a composition for promoting hair growth or inhibiting hair loss using water-soluble mastic gum and E.

Other objects or specific objects of the present invention will be set forth below.

As confirmed in the Examples and Experimental Examples below, the present invention is that a mixture of water-soluble mastic gum and its E. E. tree extract promotes the proliferation of dermal papilla cells and suppresses the expression of 5α-reductase. It is completed by checking.

Considering the experimental results as described above, the composition for promoting hair growth or inhibiting hair loss of the present invention comprises (i) water-soluble mastic gum or (ii) a mixture with its E. E. tree extract as an active ingredient. do.

In the present specification, "water-soluble mastic gum" refers to a suspension (suspension) in which mastic gum is made into fine particles (average particle diameter in the range of 1 to 999 μm) to nanoparticles (average particle diameter in the range of 1 to 999 nm) and suspended in water as a solvent ( In other words, it means that it is maintained in a dispersed state without agglomeration or precipitation in water as a solvent). Such water-soluble mastic gum can be obtained by grinding mastic gum into fine particles or nanoparticles, adding water thereto, stirring, and mixing. It may be preferable to grind the mixture (or liquid mixture) to obtain nanoparticles of mastic gum particles in the mixture. In this case, in order to stabilize the dispersed particles and prevent agglomeration and precipitation, suspending agents may be added in an appropriate amount before and after mixing the fine particles or nanoparticles with water and before and after grinding. Any suspending agent known in the art may be used, such as gum arabic, tragacanth gum, agar, karaya gum, locust bean gum, guar gum ( guar gum), xanthan gum, ghatti gum, water-soluble polymers such as pectin, cyclodextrin, glycerin or its fatty acid ester derivatives, methylcellulose or hydroxymethylcellulose Modified cellulose, various surfactants, etc. may be used. These suspending agents may be used alone or in mixture of two or more, and may be used in an appropriate amount for suspending, that is, in an amount of 20 to 200 parts by weight based on 100 weight of mastic gum. For more specific information on the preparation of water-soluble mastic gum, refer to the examples below, or Korean Patent Registration No. 10, whose title is "Method for producing an aqueous solution of mastic gum with increased dispersing and water-soluble power through nano-particleization" Reference may be made to the method disclosed in -1865712 and the like. Here, mastic gum refers to the resin (reisn) obtained from the mastic tree (Pistacia lentiscus).

In addition, in the present specification, the term "E. E. tree extract" refers to the extraction target of E. E. tree leaves, stems, above-ground parts, rhizomes, roots, underground parts, outposts, or mixtures thereof with water, lower alcohols having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) , methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N,N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or these It refers to an extract obtained by leaching using a mixed solvent of In consideration of this, any method such as chilling, reflux, warming, ultrasonic radiation, or supercritical extraction may be applied. In the case of a fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and standing still with a solvent having a different polarity, and adsorbing the crude extract to a column filled with silica gel, etc. It is meant to include the fraction obtained as a mobile phase. In addition, the meaning of the extract includes a concentrated liquid extract or solid extract from which the extraction solvent has been removed by freeze drying, vacuum drying, hot air drying, spray drying, or the like. Preferably, an extract obtained by using water, an alcohol having 1 to 4 carbon atoms or a mixed solvent thereof as an extraction solvent, more preferably a mixed solvent of water and an alcohol having 1 to 4 carbon atoms as an extraction solvent, especially a mixed solvent of ethanol and water means an extract obtained using

In addition, as used herein, the term "active ingredient" refers to a component capable of exhibiting the desired activity alone or in combination with a carrier that has no activity by itself.

In addition, in the present specification, the term "hair loss" should be understood as meaning including all symptoms classified as hair loss in the art, regardless of the direct or indirect cause of the occurrence. Specifically, it is meant to include all symptoms of hair loss due to poor blood circulation, excessive sebum secretion due to excessive production of dihydrotestosterone, deterioration of scalp function due to peroxide or bacteria, aging, genetic factors, stress, and complex actions thereof. .

In the composition of the present invention, the mixture of the water-soluble mastic gum and the E. E. tree extract may be according to any mixing ratio, and when the following examples and experimental examples are considered, the mixture is water-soluble mastic gum 100 weight In comparison, the E. E. tree extract may be a mixture in the range of 0.1 to 10 parts by weight.

The composition of the present invention may be included in any amount (effective amount) depending on the use, formulation, compounding purpose, etc., as long as the active ingredient can exhibit ankle promoting or hair loss inhibiting activity, and a typical effective amount is based on the total weight of the composition. It will be determined within the range of 0.001 wt % to 20.0 wt %. As used herein, the term "effective amount" refers to a mammal, preferably a human, to which the subject is applied, when the composition of the present invention is administered during the administration period as suggested by a medical professional, etc., which can exhibit intended functional and pharmacological effects such as promoting hair growth. Refers to the amount of the active ingredient contained in the composition of the present invention. Such effective amounts can be determined empirically within the ordinary ability of one of ordinary skill in the art.

In addition to the active ingredient, the composition of the present invention has already been verified in safety in the art for increasing/reinforcing the effect of promoting hair growth or inhibiting hair loss, or to enhance the convenience of taking or ingestion through the addition of similar activities such as blood pressure control activity. and may further include any compound or natural extract known to have a corresponding activity. Such compounds or extracts include compounds or extracts, drugs listed in compendial documents such as pharmacopeias of each country (“Korean Pharmacopoeia” in Korea) and health functional food regulations of each country (“health functional food standards and specifications” announced by the Ministry of Food and Drug Safety in Korea). Each country's laws governing the manufacture and sale of compounds, extracts, and health functional foods that have been approved for items in accordance with the laws of each country (the "Pharmaceutical Act" in Korea) that regulate the manufacture and sale of '), and compounds or extracts whose functionality is individually recognized are included.

For example, dexpanthenol, biotin, L-menthol, zinc pyrithione, etc., which are ingredients with a function of alleviating hair loss symptoms listed in the “Standards and Test Methods for Functional Cosmetics” announced by the Ministry of Food and Drug Safety in accordance with Korean cosmetics, or with blood pressure control functions L-glutamic acid-derived GABA-containing powder, Katsuobushi oligopeptide, nattobacterial culture powder, Semoktae (snow bean) peptide complex, salmon peptide, olive leaf extract, sardine peptide, casein hydrolyzate, coenzyme Q10, grape seed enzyme decomposition extract powder, haetai oligopeptides and the like would correspond to such compounds or extracts.

In a specific embodiment, the composition of the present invention may be identified as a food composition.

The food composition of the present invention may be prepared in any form, for example, beverages such as tea, juice, carbonated beverages, and ionic beverages, processed oils such as milk and yogurt, gums, rice cakes, Korean sweets, bread, confectionery, noodles, etc. Foods, tablets, capsules, pills, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, and health functional food preparations such as bars can be prepared.

In addition, the food composition of the present invention may have any product classification in terms of legal and functional classification as long as it conforms to the enforcement laws at the time of manufacture and distribution. For example, it is a health functional food according to Korea's "Health Functional Food Act", or confectionery, beans, tea, and beverages according to each food type according to the Food Ordinance of Korea's Food Sanitation Act (Ministry of Food and Drug Safety Notification "Food Standards and Specifications") , food for special use, and the like.

The food composition of the present invention may contain food additives in addition to the active ingredients thereof. Food additives can be generally understood as substances that are added to and mixed with or infiltrated into food in manufacturing, processing, or preserving food. Food additives with guaranteed safety are limited in terms of ingredients or functions in the Food Additives Ordinance in accordance with the laws of each country that regulates the manufacture and distribution of food (“Food Sanitation Act” in Korea). In the Korean Food Additives Code (“Food Additive Standards and Specifications” notified by the Ministry of Food and Drug Safety), food additives are classified into chemically synthetic products, natural additives, and mixed preparations in terms of ingredients. It is classified into an agent, a preservative, an emulsifier, an acidulant, and a thickener.

The sweetener is used to impart appropriate sweetness to food, and both natural and synthetic ones may be used in the food composition of the present invention. Preferably, a natural sweetener is used. Examples of the natural sweetener include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavoring agents are used for the purpose of improving taste or aroma, and both natural and synthetic ones may be used. Preferably, it is a case where a natural thing is used. In the case of using a natural one, the purpose of nutritional enhancement in addition to flavor may be concurrently used. The natural flavoring agent may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, or the like, or obtained from green tea leaves, horseradish leaves, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. In addition, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, ginkgo biloba, etc. can be used. The natural flavoring agent may be a liquid concentrate or a solid extract. In some cases, a synthetic flavoring agent may be used, and the synthetic flavoring agent may include esters, alcohols, aldehydes, terpenes, and the like.

Calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid), etc. can be used as preservatives, and acacia gum, carboxymethylcellulose, xanthan gum, and pectin can be used as emulsifiers. acidulant, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and the like may be used as acidulants. Acidulant may be added so that the food composition has an appropriate acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste. As the thickening agent, a suspending agent, a settling agent, a gel-forming agent, a bulking agent, and the like can be used.

The food composition of the present invention may contain, in addition to the food additives described above, bioactive substances or minerals known in the art for the purpose of supplementing and reinforcing functionality and nutrition and with guaranteed stability as food additives.

Examples of such physiologically active substances include catechins contained in green tea and the like, vitamins such as vitamin B1, vitamin C, vitamin E, and vitamin B12, tocopherol, dibenzoylthiamine, and the like. Magnesium preparations, such as iron preparations, such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc, etc. are mentioned.

In the food composition of the present invention, the food additives as described above may be included in an appropriate amount to achieve the purpose of the addition according to the type of product.

In relation to other food additives that may be included in the food composition of the present invention, reference may be made to the national food regulations or food additives regulations.

The composition of the present invention may be identified as a pharmaceutical composition in another specific embodiment.

The pharmaceutical composition of the present invention may be prepared as an oral dosage form or a parenteral dosage form according to the route of administration by a conventional method known in the art, including a pharmaceutically acceptable carrier in addition to the active ingredient. Here, the route of administration may be any suitable route including topical route, oral route, intravenous route, intramuscular route, and direct absorption through mucosal tissue, and two or more routes may be used in combination.

Pharmaceutically acceptable carriers are well known in the art depending on the route of administration or formulation, and specifically, reference may be made to the pharmacopeias of each country including the "Korea Pharmacopoeia".

When the pharmaceutical composition of the present invention is prepared as an oral dosage form, powders, granules, tablets, pills, dragees, capsules, liquids, gels, syrups, suspensions, wafers, together with a suitable carrier according to methods known in the art. It can be prepared in a formulation such as Examples of suitable carriers include sugars such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol, and xylitol, starches such as corn starch, potato starch, wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Cellulose such as hydroxypropylmethylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol, grease Serol etc. are mentioned. In the case of formulation activity, an appropriate binder, lubricant, disintegrant, colorant, diluent, etc. may be included as needed. Suitable binders include starch, magnesium aluminum silicate, starch ferrist, gelatin, methylcellulose, sodium carboxymethylcellulose, polyvinylpyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax, and the like, and oleic acid as lubricant. sodium, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, magnesium salts and calcium salts thereof, polyethylene glycol, etc., and the disintegrant include starch, methyl cellulose , agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt. Examples of the diluent include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine, and the like.

When the pharmaceutical composition of the present invention is prepared for parenteral use, it may be formulated in the form of injections, transdermal administrations, nasal inhalants and suppositories together with suitable carriers according to methods known in the art. When formulated as an injection, an aqueous isotonic solution or suspension may be used as a suitable carrier, and specifically, PBS (phosphate buffered saline) containing triethanolamine, sterile water for injection, or isotonic solution such as 5% dextrose may be used. . When formulated for transdermal administration, it can be formulated in the form of ointments, creams, lotions, gels, external solutions, pasta agents, liniment agents, air rolls, and the like. In the case of nasal inhalants, it can be formulated in the form of an aerosol spray using a suitable propellant such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, and the like. witepsol), tween 61, polyethylene glycols, cacao fat, laurin fat, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearate, sorbitan fatty acid esters, and the like can be used.

Specific formulations of pharmaceutical compositions are known in the art, and reference may be made to, for example, Remington's Pharmaceutical Sciences (19th ed., 1995). This document is considered a part of this specification.

A preferred dosage of the pharmaceutical composition of the present invention is in the range of 0.001 mg/kg to 10 g/kg per day, preferably 0.001 mg/kg to 1 g, depending on the patient's condition, weight, sex, age, patient's severity, and administration route. It can be in the range /kg. Administration may be performed once or divided into several times a day. Such dosages should not be construed as limiting the scope of the invention in any respect.

In another specific embodiment, the composition of the present invention can be identified as a cosmetic composition.

Even when the composition of the present invention is identified as a cosmetic composition, the cosmetic composition can be classified into any product in terms of its use or by law, and specifically, uses such as alleviating hair loss, promoting hair growth, improving skin troubles, and improving atopic dermatitis. It may be functional cosmetics, non-functional general cosmetics, and the like. The product form may also take any product form, specifically solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, shampoo, surfactant-containing cleansing, oil, powder foundation, emulsion foundation , wax foundation, spray, etc. In the specific product form, it may be in the form of a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder.

The cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the active ingredient, for example, conventional adjuvants such as stabilizers, solubilizers, surfactants, vitamins, pigments and fragrances, and carriers.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, fatty acid ester of sorbitan, etc. may be used.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, polyoxyethylene sorbitan ester, microcrystals Sex cellulose, aluminum metahydroxide, bentonite, agar, and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

The cosmetic composition of the present invention can be prepared according to a method for preparing a cosmetic composition conventionally performed in the art, except that it contains an active ingredient that exhibits hair growth promoting activity and the like.

As described above, according to the present invention, it is possible to provide a composition for promoting hair growth or inhibiting hair loss using water-soluble mastic gum and E. E. tree extract. The composition for promoting hair growth or inhibiting hair loss of the present invention may be commercialized as a functional food, drug, functional cosmetic, or the like.

1 and 2 are results of evaluation of the proliferation efficacy of dermal papilla cells.
3 and 4 are results of evaluating the expression level of 5α-reductase.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these Examples and Experimental Examples.

<Example> Efficacy of promoting hair growth or inhibiting hair loss of a mixture of water-soluble mastic gum and E. E. extracts

1. Preparation of Samples

1.1 Preparation of Soluble Mastic Gum

The mastic gum powder was visually inspected for color confirmation and foreign matter inspection. After that, 20% by weight of the selected mastic gum powder, 75.7% by weight of purified water, 4% by weight of cyclodextrin, and 0.3% by weight of additive (gatti gum) were added, and 30-50° C. It was dispersed so as to have a particle size of about 20-50 μm for 30-60 minutes at 2,000-3,000 rpm. The dispersion of the mastic gum was carried out for 3 to 6 hours using a bead mill (Dentech Co., Ltd.) at 20 to 40° C. so that the final dispersed particles became an average of 600 to 1,000 nm. Then, it was filtered through a 40-60 μm housing filter to obtain an aqueous solution of mastic gum in a suspension state. In the experiment below, this water-soluble mastic gum was used as it is.

1.2 Preparation of Ecklonia extract

After extracting E. E. E. tree (extraction part: leaves, twigs) with 70% alcohol by 4-8 times the weight at 70-85°C for 4-8 hours, the filtered filtrate is concentrated under reduced pressure at 60°C or lower and freeze-dried to form a powder extracts were obtained.

2. Hair growth promotion or hair loss inhibition efficacy test

2.1 Efficacy of proliferation of dermal papilla cells

^{Dermal papilla cells (PromoCell) were prepared in a 96-well plate at 1X10 3} /well using DMEM medium containing 1% FBS (Fetal Bovine Serum) and cultured at 37°C in an incubator for 24 hours. The following sample concentrations were treated, cultured for 24 to 96 hours, and the change in the growth rate was evaluated by observing the absorbance at 450 nm using the D-Plus CCK cell viability assay kit (Dongin Biotech Co., Ltd.). The results for the water-soluble mastic gum are shown in FIG. 1 as a percentage compared to the untreated group (1% FBS), and the results for the mixture of the water-soluble mastic gum and E. E. tree extract are shown in FIG. 2 . Minoxidil (Mnx, 100 μM) and/or finasteride (1 μM) were used as positive controls.

Referring to Figures 1 and 2, the mixture of water-soluble mastic gum and its E. E. tree extract stimulated the proliferation of dermal papilla cells in a concentration-dependent manner.

2.2 Measurement of expression level of 5α-reductase, a factor related to hair loss

^{The dermal papilla cells were prepared at 1X10 3} /well using DMEM medium containing 1% FBS (Fetal Bovine Serum) in a 96-well plate and cultured for 24 hours in an incubator at 37°C, and then the samples were processed by concentration and treated for 48 hours and 72 hours. After the time incubation, the culture medium was recovered and the expression level of 5α-reductase (5α-reductase, 5AR) involved in hair loss was evaluated using an ELISA assay (Human 5AR ELISA kit, Cusabio). The results for the water-soluble mastic gum are shown in FIG. 3 as a percentage compared to the untreated group (1% FBS), and the results for the mixture of the water-soluble mastic gum and E. E. tree extract are shown in FIG. 4 . Here again, minoxidil (Mnx, 100 μM) and/or finasteride (1 μM) were used as positive controls.

Referring to Figures 3 and 4, the mixture of water-soluble mastic gum and its E. E. tree extract inhibited the expression of 5α-reductase in a concentration-dependent manner.

2.3 Statistical Analysis

The dermal papilla cell proliferation efficacy and 5α-reductase expression change rate of the untreated group and the group treated with minoxidil, phytasteride and mastic gum, E. tree extract, and mixture were compared for each time period. To verify the statistical significance, an independent t-test was performed, and it was converted into a p-value and expressed. All of the above statistical processing were analyzed using the Excel program.

Claims (5)

As an active ingredient, including a mixture of water-soluble mastic gum and E. E. tree extract,
The water-soluble mastic gum is a suspension in which the mastic gum is made into fine particles (average particle diameter in the range of 1 to 999 μm) to nanoparticles (average particle diameter in the range of 1 to 999 nm) and suspended in water as a solvent,
Food composition for promoting hair growth or inhibiting hair loss .
According to claim 1,
The E. E. tree extract is a food composition, characterized in that water, ethanol, or a mixed solvent extract thereof.
As an active ingredient, including a mixture of water-soluble mastic gum and E. E. tree extract,
The water-soluble mastic gum is a suspension in which the mastic gum is made into fine particles (average particle diameter in the range of 1 to 999 μm) to nanoparticles (average particle diameter in the range of 1 to 999 nm) and suspended in water as a solvent,
A pharmaceutical composition for treating or preventing hair loss .
4. The method of claim 3,
The E. E. tree extract is a pharmaceutical composition, characterized in that water, ethanol, or a mixed solvent extract thereof.
As an active ingredient, including a mixture of water-soluble mastic gum and E. E. tree extract,
The water-soluble mastic gum is a suspension in which mastic gum is made into fine particles (average particle size in the range of 1-999 μm) to nanoparticles (average particle diameter in the range of 1-999 nm) and suspended in water as a solvent,
The Ecklonia tree extract is water, ethanol, or a mixed solvent extract thereof.
A cosmetic composition for promoting hair growth or inhibiting hair loss.

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