KR20180120915A - Composition for Protection of Brain Neuronal Cells Using an Extract of Schizandra chinensis and an Extract of Ribes fasciculatum - Google Patents
Composition for Protection of Brain Neuronal Cells Using an Extract of Schizandra chinensis and an Extract of Ribes fasciculatum Download PDFInfo
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- KR20180120915A KR20180120915A KR1020170054871A KR20170054871A KR20180120915A KR 20180120915 A KR20180120915 A KR 20180120915A KR 1020170054871 A KR1020170054871 A KR 1020170054871A KR 20170054871 A KR20170054871 A KR 20170054871A KR 20180120915 A KR20180120915 A KR 20180120915A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/322—Foods, ingredients or supplements having a functional effect on health having an effect on the health of the nervous system or on mental function
Abstract
Description
본 발명은 오미자 추출물(Schizandra chinensis)과 칠해목(Ribes fasciculatum) 추출물을 이용한 뇌신경세포 보호용 조성물에 관한 것이다.The present invention relates to a composition for protecting brain cells using Schizandra chinensis extract and Ribes fasciculatum extract.
세계의 여러 국가가 점차 고령화 사회로 접어들면서 뇌졸중(stroke), 파킨슨씨병(Parkinson's disease, PD), 알츠하이머(치매) 등 각종 퇴행성 뇌질환에 시달리는 사람들이 급증하고 있으며, 그 비용도 천문학적으로 소요되고 있다. 2015년 통계청 자료에 따른 국내 사망원인을 살펴보면, 악성신생물(암), 심장질환, 뇌혈관 질환, 폐렴 등 뇌질환으로 인한 사망률이 3위를 차지하고 있다. 생명 유지에 필수적인 정보를 주관하는 뇌는 신경신호에 이상이 생기면 생명에 치명적인 영향을 끼치는 각종 신경성 장애를 일으킨다. 뇌질환을 치료하는 효과적인 방법이 없는 현 실정에서는 삶의 질저하 및 막대한 의료비의 지출 등으로 주변 가족에게 상당한 정신적인 부담을 주고 있다. 이러 한 문제의 심각성을 인지하여 세계 각국은 범국민적인 관심속에 집중적인 해결방안을 모색하고 있다. As countries around the world become more and more aged, the number of people suffering from degenerative brain diseases such as stroke, Parkinson's disease (PD), and Alzheimer's (dementia) is increasing rapidly and the cost is also astronomical . According to the statistics of the National Statistical Office (NSO) in 2015, death rate from brain diseases such as malignant neoplasm (cancer), heart disease, cerebrovascular disease, and pneumonia accounts for the third place. The brain, which is responsible for information essential to life support, causes neurotic disorders that have a life-threatening effect if neural signals are abnormal. In the current situation where there is no effective way to treat brain diseases, it causes considerable psychological burden on the family members due to the deterioration of quality of life and the expense of huge medical expenses. Recognizing the seriousness of these problems, countries around the world are looking for solutions that are focused on the people.
신경세포는 발생 및 시냅스를 재구성하는 과정에서 끊임없이 세포사멸하며, 스트레스와 세포독성 약물에 의한 세포사멸이 퇴행성 뇌질환의 주요 요인 중 하나이다. 퇴행성 뇌질환은 나이가 들어감에 따라 발생하는 뇌질환으로 환경적, 유전적 요인의 누적으로 인하여 발병하는 것으로 알려져 있다. 뇌와 척수의 특정 신경세포군이 서서히 그 기능을 잃고 뇌신경계의 정보전달에 가장 중요한 뇌신경세포의 사멸, 뇌신경세포와 뇌신경세포 사이의 정보를 전달하는 시냅스 형성이나 기능상의 문제, 뇌신경의 전기적 활동성의 이상적 증가나 감소로 인하여 야기된다. 뇌와 척수의 신경세포들은 위치에 따라 매우 다양한 기능을 하고 있어 어느 부위의 신경세포들이 먼저 손상되고 기능을 잃어가느냐에 따라, 또 이러한 기능장애가 어떤 형태로 진행되는가에 따라 매우 다양한 임상 양상을 보이게 된다. 퇴행성 뇌질환은 나타나는 주요 증상과 침범되는 뇌부위를 고려하여 구분 할 수 있으며, 알츠하이머병(Alzheimer's disease; AD), 파킨슨병(Parkinson's disease; PD), 헌팅톤병(Huntington's disease: HD), 다발성경화증 (Multiple sclerosis; MS), 루게릭병으로 알려진 근위축성 측삭 경화증(Amyotrophic lateral sclerosis: ALS) 등이 포함된다. 여러 인자 중 산화적 스트레스는 퇴행성 신경질환의 유발원인과 많은 연관 관계를 가진 것으로 알려져 있다. 뇌는 체중의 2% 밖에 되지 않지만 전체 산소소비량의 20% 정도를 소모하고 있기 때문에 산소 이용률이 높아 체내에서 가장 많은 활성산소가 발생하는 부위이다. 최근 연구에 따르면 만성적인 스트레스 및 산화적 스트레스는 시상하부-뇌 하수체-부신피질계, 해마, 선조체, 흑질 그리고 전뇌피질 부위에서 산화적 스트레스를 유발하여 세포사멸을 증가시키고 뉴런 및 성장인자를 감소시켜 퇴행성 뇌질환을 초래하는 것으로 보고되었다(Floyd RA. Proc Soc Exp Biol Med. 1999 Dec;222(3):236-45.;Wang JY et al., Curr Pharm Des. 2006;12(27):3521-33).Neurons are constantly killed in the process of reconstitution and synapse reconstruction, and stress and apoptotic cell death are one of the major factors of degenerative brain disease. It is known that degenerative brain diseases are caused by the accumulation of environmental and genetic factors. Neurons in the brain and spinal cord slowly lose their functions and are most important for the transmission of information in the nervous system Neuronal death of the brain, synapse formation or functional issues that transmit information between neuronal and neuronal cells, ideal for the electrical activity of the brain Increase or decrease. Neurons in the brain and spinal cord have a wide variety of functions depending on their location, and they show a wide variety of clinical features depending on which part of the neuron is damaged first, the function is lost, . Degenerative brain diseases can be distinguished by considering the main symptoms that appear and the area of the affected brain. Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), multiple sclerosis Multiple sclerosis (MS), Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease. Oxidative stress among many factors is known to be related to the cause of degenerative neuropathy. The brain is only 2% of body weight, but consumes about 20% of the total oxygen consumption, so it has the highest oxygen utilization rate and is the area where the most active oxygen occurs in the body. Recent studies have shown that chronic stress and oxidative stress induce oxidative stress in the hypothalamus-brainstem-adrenal cortex, hippocampus, hippocampus, striatum, and blackheads, leading to increased cell death, decreased neuronal and growth factors 2006; 12 (27): 282-9. [CrossRef], [Web of Science ®] View all references) 3521-33).
또한 뇌질환 중 뇌혈관 질환은 뇌출혈, 뇌졸중 등 혈관의 기능 이상에 따른 뇌질환으로, 산소와 에너지원의 공급 저하에 의하여 발병한다. 신경세포는 산소와 에너지원으로 글루코스(glucose)만을 이용한다. 그러나 뇌에는 글리코겐(glycogen)과 같은 저장기능이 없어 혈류를 막는 것만으로 에너지원이 완전히 차단되게 된다. 일반적으로 정상인의 혈당치는 80mg/를 유지하지만 혈당치가 20mg/가 되면 혼수상태가 된다. 또한 저산소 상태에서는 여러 활성산소가 유도되기 때문에 뇌신경세포에서 산소와 함께 글루코스의 공급이 차단되면 신경세포의 기능이 마비되고 뇌신경세포의 사멸로 연결된다(Muresan Aet al., Med Food. 2013 Sep;16(9):831-8.; Simran S et al., Nature Reviews Cancer 2014 14, 709721; Manzanero S et al., Neurochem Int. 2013 Apr;62(5):712-8.) 뇌혈관 질환에 의한 뇌조직 손상은 혈관성 치매 등의 질병으로 진행하게 된다.In addition, cerebrovascular disease is a cerebrovascular disease caused by abnormal function of blood vessels such as cerebral hemorrhage and stroke, and is caused by a decrease in supply of oxygen and energy sources. Neurons use only glucose as oxygen and energy source. However, the brain does not have a storage function such as glycogen, and the energy source is completely blocked only by blocking the blood flow. Normally, the blood glucose level of normal people is maintained at 80 mg / liter, but when the blood glucose level is 20 mg / liter, it becomes coma. In addition, since hypoxia induces multiple reactive oxygen species, blocking of glucose supply with oxygen in brain cells results in paralysis of nerve cell function and death of neuronal cells (Muresan Aet al., Med Food. 2013 Sep; 16 (9): 831-8 .; Simran S et al., Nature Reviews Cancer 2014 14, 709721; Manzanero S et al., Neurochem Int. 2013 Apr; 62 (5): 712-8) Brain tissue damage progresses to diseases such as vascular dementia.
그밖에도 안정한 분자상태인 기저삼중항산소(ground state triplet oxygen)가 체내 효소계, 환원대사, 화학약품, 공해물질, 광화학반응 등의 각종 물리적 화학적, 환경적 요인 등에 의하여 superdxide radical(O2-), hydroxyl radical(HO), 과산화수소(hydrogen peroxide, H2O2), singlet oxigen(1O2)과 같은 반응성이 매우 큰 활성산소 (active oxygen)으로 전환되면 생체에 치명적인 산소독성을 신경에 일으킨다. In addition, ground state triplet oxygen, which is a stable molecular state, can be converted to superdoxide radical (O2-), hydroxyl (OH), and hydroxyl radical by various physical and chemical factors such as enzymatic system, reductive metabolism, chemicals, pollutants, Conversion to highly reactive active oxygen such as radicals (HO), hydrogen peroxide (H2O2), and singlet oxigen (1O2) can cause fatal oxygen toxicity to the living body.
신경세포의 산화적 스트레스는 미토콘드리아에서의 시토크롬 C의 유리와 카스파아제-3 활성화를 일으켜 세포사멸을 유발한다. 또한 활성산소종은 글루타메이트, 특히 NMDA 수용체를 활성화하여 메타보트로피칼 케스케이드(metabotrophical cascade)에 의한 Ca2+이온의 증가를 야기하고, 세포내 Ca2+ 의 증가는 카스파아제-2를 활성화하여 DNA를 손상시킨다. (Bonini P et al., J Neurosci Res. 2004 Jan 1;75(1):83-95.; Polster BM and Fiskum G. J Neurochem. 2004 Sep;90(6):1281-9.; Gorman AM et al., Neuroreport. 1998 Jul 13;9(10):R49-55). Ca2+ 이온 항상성의 파괴로 인한 흥분성 신경독성, 내형질 세망과 미토콘드리아의 기능불능, 산화적 스트레스에 의한 DNA 손상 등으로 인해 세포사멸이 일어나게 된다(Wei et al., 1999, Toxicology 134:117-26.). Oxidative stress in neurons causes cytochrome C release and caspase-3 activation in mitochondria, leading to apoptosis. In addition, reactive oxygen species activate glutamate, particularly the NMDA receptor, resulting in an increase of Ca2 + ion by metabotrophical cascade, and increase of intracellular Ca2 + activates caspase-2 to damage DNA. Polymer BM and Fiskum G. J Neurochem. 2004 Sep; 90 (6): 1281-9 .; Gorman AM et < RTI ID = 0.0 > al., Neuroreport, 1998 Jul 13; 9 (10): R49-55). Cellular apoptosis occurs due to excitatory neurotoxicity due to destruction of Ca2 + ion homeostasis, dysregulation of mitochondria and DNA damage due to oxidative stress (Wei et al., 1999, Toxicology 134: 117-26). ).
이처럼 활성산소는 퇴행성 뇌질환 및 뇌혈관질환의 발병과 진행에 큰 영향을 끼치기 때문에 SH-SY5Y 세포들에 대한 과산화수소 처리 등 ROS 생성 세포사멸 조건에서 신경세포 보호 효과를 확인하는 실험은 뇌졸중 및 퇴행성 뇌질환의 신경손상을 억제하고 그 치료법을 탐색하는 데 있어서 중요한 연구 방법으로 사용되고 있다. Since ROS plays a major role in the development and progression of degenerative brain diseases and cerebrovascular diseases, experiments to confirm the protective effect of neurons in ROS-producing cell death conditions, such as treatment with hydrogen peroxide for SH-SY5Y cells, Has been used as an important research tool in inhibiting nerve injury of the disease and searching for its treatment.
본 발명에서는 오미자와 칠해목 추출물의 신경세포 보호 효과를 확인하였다.In the present invention, the protective effects of neurons on the extracts of Omija and pine wood were confirmed.
본 발명의 목적은 오미자 추출물과 칠해목 추출물을 이용한 뇌신경세포 보호용 조성물을 제공하는 데 있다.It is an object of the present invention to provide a composition for protecting brain cells using Omija extract and Pyrrhizae extract.
본 발명의 다른 목적이나 구체적인 목적은 이하에서 제시될 것이다.Other and further objects of the present invention will be described below.
본 발명자들은 아래의 실시예에서 확인되는 바와 같이, 인간신경모세포종 SH-SY5Y 세포주를 이용하여 오미자 추출물과 칠해목 추출물이 뇌신경세포에 대한 세포독성의 유무와, 과산화수소에 의한 뇌신경세포 사멸 억제 효과 유무를 살펴봤는데, 세포독성이 없으면서 농도 의존적으로 뇌신경세포 사멸 억제 효과를 가짐을 확인하였다.As shown in the following examples, the inventors of the present invention used SH-SY5Y cell line of human neuroblastoma to determine whether cytotoxic effect of Omiza extract and Pyrrhiza pratensis extract on brain nerve cells and inhibition of neuronal cell death by hydrogen peroxide As a result, it was confirmed that the compound had cytotoxic and cytotoxic effect in a concentration dependent manner.
이러한 실험 결과에 고려할 때, 일 측면에 있어서, 본 발명은 오미자 추출물, 칠해목 추출물 또는 이들의 혼합물을 유효성분으로 포함하는 뇌신경세포 보호용 조성물로 파악할 수 있고, 다른 측면에 있어서 오미자 추출물, 칠해목 추출물 또는 이들의 혼합물을 유효성분으로 포함하는 뇌신경세포의 사멸을 수반하는 질환의 개선용 조성물로 파악할 수 있다.In one aspect, the present invention can be understood as a composition for protecting brain cells comprising an extract of Omija, a extract of Phellodendus japonica or a mixture thereof as an active ingredient. In another aspect, the present invention provides a composition for protecting cells of Omine, Or a mixture thereof, as an active ingredient, for the improvement of diseases accompanied with the death of brain cells.
본 명세서에서, "오미자 또는 칠해목 추출물"은 추출 대상인 오미자 또는 칠해목 줄기, 잎, 열매, 꽃, 뿌리, 이들의 혼합물 등을 물, 탄소수 1 내지 4의 저급 알콜(메탄올, 에탄올, 부탄올 등), 메틸렌클로라이드, 에틸렌, 아세톤, 헥산, 에테르, 클로로포름, 에틸아세테이트, 부틸아세테이트, N,N-디메틸포름아미드(DMF), 디메틸설폭사이드(DMSO), 1,3-부틸렌글리콜, 프로필렌글리콜 또는 이들의 혼합 용매를 사용하여 침출하여 얻어진 추출물, 이산화탄소, 펜탄 등 초임계 추출 용매를 사용하여 얻어진 추출물 또는 그 추출물을 분획하여 얻어진 분획물을 의미하며, 추출 방법은 활성물질의 극성, 추출 정도, 보존 정도를 고려하여 냉침, 환류, 가온, 초음파 방사, 초임계 추출 등 임의의 방법을 적용할 수 있다. 분획된 추출물의 경우 추출물을 특정 용매에 현탁시킨 후 극성이 다른 용매와 혼합·정치시켜 얻은 분획물, 상기 조추출물을 실리카겔 등이 충진된 칼럼에 흡착시킨 후 소수성 용매, 친수성 용매 또는 이들의 혼합 용매를 이동상으로 하여 얻은 분획물을 포함하는 의미이다. 또한 상기 추출물의 의미에는 동결건조, 진공건조, 열풍건조, 분무건조 등의 방식으로 추출 용매가 제거된 농축된 액상의 추출물 또는 고형상의 추출물이 포함된다. 바람직하게는 추출용매로서 물, 에탄올 또는 이들의 혼합 용매를 사용하여 얻어진 추출물, 더 바람직하게는 추출용매로서 물과 에탄올의 혼합 용매를 사용하여 얻어진 추출물을 의미한다.In the present specification, the term " omija or pine wood extract " means water, a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, butanol, etc.) Methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, N-dimethylformamide (DMF), dimethylsulfoxide (DMSO), 1,3-butylene glycol, Extracts obtained by leaching using a mixed solvent of carbon dioxide and pentane, or fractions obtained by fractionating the extracts obtained by using a supercritical extraction solvent such as pentane, Any method such as cold rolling, refluxing, heating, ultrasonic irradiation, supercritical extraction, etc. can be applied. In the case of the fractionated extract, a fraction obtained by suspending the extract in a specific solvent and mixing and leaving with a solvent having a different polarity, the crude extract is adsorbed on a column packed with silica gel, and then a hydrophobic solvent, a hydrophilic solvent, Quot; means fractions obtained as a mobile phase. Also, the meaning of the extract includes a concentrated liquid extract or a solid extract in which the extraction solvent is removed by a method such as freeze drying, vacuum drying, hot air drying, spray drying and the like. Preferably an extract obtained by using water, ethanol or a mixed solvent thereof as an extraction solvent, and more preferably an extract obtained by using a mixed solvent of water and ethanol as an extraction solvent.
또 본 명세서에서 "유효성분"이란 단독으로 목적하는 활성을 나타내거나 또는 그 자체는 활성이 없는 담체와 함께 활성을 나타낼 수 있는 성분을 의미한다.In the present specification, the term " active ingredient " alone means an ingredient which exhibits the desired activity or which can exhibit activity together with a carrier which is not itself active.
또 본 명세서에서, "뇌신경세포의 보호"는 아래에서 정의되는 뇌신경세포의 사멸을 수반하는 질환에서 뇌신경세포 사멸을 억제하는 의미 이외에 노화로 인한 뇌신경세포의 사멸을 억제하는 의미를 포함한다. 뇌신경세포의 사멸 억제는 곧 인지 능력의 개선을 가져오므로, 상기 "뇌신경세포의 보호"는 인지 능력 개선의 의미로도 이해될 수 있다. In the present specification, " protection of neuronal cells " includes the meaning of inhibiting neuronal cell death in diseases accompanied by death of neuronal cells defined below, as well as inhibiting the death of neuronal cells due to aging. Since the inhibition of the extinction of neuronal cells leads to the improvement of the cognitive ability, the above " protection of the neuronal cells "
또 본 명세서에서, "뇌신경세포의 사멸을 수반하는 질환의 개선"는 아래에서 정의되는 뇌신경세포의 사멸을 수반하는 질환의 증상 경감, 치료, 그러한 질환의 예방(발병 억제 또는 지연)을 포함하는 의미이다.As used herein, the term " amelioration of diseases accompanied by apoptosis of neuronal cells " is intended to mean amelioration of symptoms, treatment of diseases accompanied by apoptosis of neuronal cells as defined below, prevention of such diseases to be.
또 본 명세서에서, "뇌신경세포의 사멸을 수반하는 질환"은 알츠하이머병(Alzheimer's disease; AD), 파킨슨병(Parkinson's disease; PD), 헌팅톤병(Huntington's disease: HD), 다발성경화증 (Multiple sclerosis; MS), 루게릭병으로 알려진 근위축성 측삭 경화증(Amyotrophic lateral sclerosis: ALS) 등의 퇴행성 뇌질환과 혈관성 치매 등의 허혈성 뇌질환을 포함한다. In the present specification, the term " a disease accompanied by the death of neuronal cells " includes Alzheimer's disease (AD), Parkinson's disease (PD), Huntington's disease (HD), Multiple sclerosis ), Amyotrophic lateral sclerosis (ALS), also known as Lou Gehrig's disease, and ischemic brain diseases such as vascular dementia.
본 발명의 조성물에서 그 유효성분은 뇌신경세포 보호 효과 등을 나타낼 수 있는 한, 용도, 제형, 배합 목적 등에 따라 임의의 양(유효량)으로 포함될 수 있는데, 통상적인 유효량은 조성물 전체 중량을 기준으로 할 때 0.001 중량 % 내지 20.0 중량 % 범위 내에서 결정될 것이다. 여기서 "유효량"이란 그 적용 대상인 포유동물 바람직하게는 사람에게 의료 전문가 등의 제언에 의한 투여 기간 동안 본 발명의 조성물이 투여될 때, 뇌신경세포 보호 효과 등 의도한 기능적·약리학적 효과를 나타낼 수 있는, 본 발명의 조성물에 포함되는 유효성분의 양을 말한다. 이러한 유효량은 당업자의 통상의 능력 범위 내에서 실험적으로 결정될 수 있다. In the composition of the present invention, the active ingredient may be contained in an arbitrary amount (effective amount) as long as it can exhibit a neuroprotective effect on the cell, etc., depending on the purpose of use, formulation, compounding purpose and the like. To < RTI ID = 0.0 > 0. < / RTI > The term " effective amount " as used herein means an amount of a compound capable of exhibiting a functional or pharmacological effect, such as a protective effect on brain cells, when the composition of the present invention is administered to a mammal, preferably a human, , And the amount of the active ingredient contained in the composition of the present invention. Such effective amounts can be determined experimentally within the ordinary skill of those skilled in the art.
본 발명의 조성물은 유효성분 이외에, 뇌신경세포 보호 효과의 상승·보강을 위하여 또는 항스트레스 활성, 피로 개선 활성 활성 등 유사활성의 부가를 통한 복용이나 섭취의 편리성을 증진시키기 위하여, 당업계에서 이미 안전성이 검증되고 해당 활성을 갖는 것으로 공지된 임의의 화합물이나 천연 추출물을 추가로 포함할 수 있다. 이러한 화합물 또는 추출물에는 각국 약전(한국에서는 "대한민국약전"), 각국 건강기능식품공전(한국에서는 식약처 고시인 "건강기능식품 기준 및 규격"임) 등의 공정서에 실려 있는 화합물 또는 추출물, 의약품의 제조·판매를 규율하는 각국의 법률(한국에서는 "약사법"임)에 따라 품목 허가를 받은 화합물 또는 추출물, 건강기능식품의 제조·판매를 규율하는 각국 법률(한국에서는 「건강기능식품에관한법률」임)에 따라 기능성이 인정된 화합물 또는 추출물이 포함된다. 예컨대 한국 「건강기능식품에관한법률」에 따라 '인지 능력 개선'으로 기능성을 인정받은 Lactobacillus Helveticus 발효물, 도라지 추출물(DRJ-AD), 참당귀 뿌리 추출물, 참당귀 추출 분말, 포스파티딜세린 등과 '피로 개선'으로 기능성을 인정받은 발효 생성 아미노산 복합물, 헛개나무 과병 추출물, 홍경천 추출물 등과, '항스트레스'로 기능성을 인정받은 L-테아닌, 아쉬아간다 추출물, 유단백가수분해물, 돌외 잎 추출물 등이 이러한 화합물 또는 추출물에 해당할 것이다.The composition of the present invention may be used in the art to increase or supplement the protective effect of neuronal cells or to enhance the convenience of taking or ingesting by adding similar activities such as antistress activity and fatigue improving activity activity And may further comprise any compound or natural extract known to be safe and have that activity. Such compounds or extracts include compounds or extracts listed in the official pamphlet of each country's pharmacopeia ("Korea Pharmacopoeia" in Korea), each country's health functional foods (in Korea, "Health Functional Food Standards and Specifications" (The "Act on Health Functional Foods" in Korea), which regulates the manufacture and sale of compounds or extracts and health functional foods approved by the respective countries in accordance with the laws of the respective countries ("Pharmaceutical Affairs Law" in Korea) &Quot;).≪ / RTI > For example, Lactobacillus Helveticus fermented product, Doraji extract (DRJ-AD), Chrysanthemum anguillarum root extract, Chrysanthemum orientalis extract powder, phosphatidylserine and the like which have been recognized as functional by the improvement of cognitive ability according to the Korean Health Functional Food Act, And the extracts of L-theanine, Ash-Ganda extract, milk protein hydrolyzate and off-shore leaf extract, which have been recognized as "anti-stress" Or extract.
이러한 화합물 또는 천연 추출물은 본 발명의 조성물에 그 유효성분과 함께 하나 이상 포함될 수 있다.Such compounds or natural extracts may be included in the compositions of the present invention in combination with one or more of their active ingredients.
본 발명의 조성물은 구체적인 양태에 있어서, 식품 조성물로서 파악할 수 있다.In a specific embodiment, the composition of the present invention can be identified as a food composition.
본 발명의 식품 조성물은 어떠한 형태로도 제조될 수 있으며, 예컨대 차, 쥬스, 탄산음료, 이온음료 등의 음료류, 우유, 요구루트 등의 가공 유류(乳類), 껌류, 떡, 한과, 빵, 과자, 면 등의 식품류, 정제, 캡슐, 환, 과립, 액상, 분말, 편상, 페이스트상, 시럽, 겔, 젤리, 바 등의 건강기능식품 제제류 등으로 제조될 수 있다. The food composition of the present invention can be prepared in any form and can be used in various forms such as beverages such as tea, juice, carbonated beverage, ionic drink, processed milk such as milk and request route, gum, rice cake, Such as confectionery, cotton, etc., tablets, capsules, rings, granules, liquids, powders, flakes, pastes, syrups, gels, jellies, bars and the like.
또 본 발명의 식품 조성물은 법률상·기능상의 구분에 있어서 제조·유통 시점의 시행 법규에 부합하는 한 임의의 제품 구분을 띨 수 있다. 예컨대 한국 「건강기능식품에관한법률」에 따른 건강기능식품이거나, 한국 「식품위생법」의 식품공전(식약처 고시 「식품의 기준 및 규격」)상 각 식품유형에 따른 과자류, 두류, 다류, 음료류, 특수용도식품 등일 수 있다.In addition, the food composition of the present invention may be classified into any product category as long as it meets the laws and regulations on the time of manufacture and distribution in the legal and functional category. For example, it is a health functional food according to the 「Health Functional Food Act」 in Korea, or a food functional food according to the Korean Food Sanitation Law (Food Standards and Specifications) , Special-purpose food, and the like.
본 발명의 식품 조성물에는 그 유효성분 이외에 식품첨가물이 포함될 수 있다. 식품첨가물은 일반적으로 식품을 제조, 가공 또는 보존함에 있어 식품에 첨가되어 혼합되거나 침윤되는 물질로서 이해될 수 있는데, 식품과 함께 매일 그리고 장기간 섭취되므로 그 안전성이 보장되어야 한다. 식품의 제조·유통을 규율하는 각국 법률(한국에서는 「식품위생법」임)에 따른 식품첨가물공전에는 안전성이 보장된 식품첨가물이 성분 면에서 또는 기능 면에서 한정적으로 규정되어 있다. 한국 식품첨가물공전(식약처 고시 「식품첨가물 기준 및 규격」)에서는 식품첨가물이 성분 면에서 화학적 합성품, 천연 첨가물 및 혼합 제제류로 구분되어 규정되어 있는데, 이러한 식품첨가물은 기능 면에 있어서는 감미제, 풍미제, 보존제, 유화제, 산미료, 점증제 등으로 구분된다. The food composition of the present invention may contain food additives in addition to the active ingredients thereof. Food additives are generally understood to be substances that are added to foods and mixed or infiltrated into food in the manufacture, processing or preservation of food, and their safety must be ensured since they are ingested daily with food and for long periods of time. In food additives according to the laws of the respective countries ("Food Sanitation Act" in Korea) regulating the manufacture and distribution of food, food additives with safety are specified in terms of ingredient or function. In the Food Additives Code of Korea (Food Additives Standards and Standards), the food additives are classified into chemical compounds, natural additives and mixed preparations in terms of ingredients. Such food additives are classified into sweeteners, flavors Preservatives, emulsifiers, acidulants, and thickeners.
감미제는 식품에 적당한 단맛을 부여하기 위하여 사용되는 것으로, 천연의 것이거나 합성된 것을 사용할 수 있다. 바람직하게는 천연 감미제를 사용하는 경우인데, 천연 감미제로서는 옥수수 시럽 고형물, 꿀, 수크로오스, 프룩토오스, 락토오스, 말토오스 등의 당 감미제를 들 수 있다. A sweetener is used to impart a sweet taste suitable for foods, and natural or synthetic sweeteners can be used. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.
풍미제는 맛이나 향을 좋게 하기 위하여 사용될 수 있는데, 천연의 것과 합성된 것 모두 사용될 수 있다. 바람직하게는 천연의 것을 사용하는 경우이다. 천연의 것을 사용할 경우에 풍미 이외에 영양 강화의 목적도 병행할 수 있다. 천연 풍미제로서는 사과, 레몬, 감귤, 포도, 딸기, 복숭아 등에서 얻어진 것이거나 녹차잎, 둥굴레, 대잎, 계피, 국화 잎, 자스민 등에서 얻어진 것일 수 있다. 또 인삼(홍삼), 죽순, 알로에 베라, 은행 등에서 얻어진 것을 사용할 수 있다. 천연 풍미제는 액상의 농축액이나 고형상의 추출물일 수 있다. 경우에 따라서 합성 풍미제가 사용될 수 있는데, 합성 풍미제로서는 에스테르, 알콜, 알데하이드, 테르펜 등이 이용될 수 있다. Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. Examples of natural flavoring agents include those obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or those obtained from green tea leaves, Asiatica, Daegu, Cinnamon, Chrysanthemum leaves and Jasmine. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. If desired, a synthetic flavor agent may be used. As the synthetic flavor agent, esters, alcohols, aldehydes, terpenes and the like may be used.
보존제로서는 소르브산칼슘, 소르브산나트륨, 소르브산칼륨, 벤조산칼슘, 벤조산나트륨, 벤조산칼륨, EDTA(에틸렌디아민테트라아세트산) 등이 사용될 수 있고, 또 유화제로서는 아카시아검, 카르복시메틸셀룰로스, 잔탄검, 펙틴 등이 사용될 수 있으며, 산미료로서는 연산, 말산, 푸마르산, 아디프산, 인산, 글루콘산, 타르타르산, 아스코르브산, 아세트산, 인산 등이 사용될 수 있다. 산미료는 맛을 증진시키는 목적 이외에 미생물의 증식을 억제할 목적으로 식품 조성물이 적정 산도로 되도록 첨가될 수 있다.As the preservative, calcium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like can be used, and as the emulsifier, acacia gum, carboxymethyl cellulose, xanthan gum, pectin And acidulant, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid and the like may be used as the acidulant. The acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.
점증제로서는 현탁화 구현제, 침강제, 겔형성제, 팽화제 등이 사용될 수 있다.Examples of the thickening agent include suspending agents, sedimentation agents, gel-forming agents, bulking agents and the like.
본 발명의 식품 조성물은 전술한 바의 식품첨가물 이외에, 기능성과 영양성을 보충, 보강할 목적으로 당업계에 공지되고 식품첨가물로서 안정성이 보장된 생리활성 물질이나 미네랄류를 포함할 수 있다.The food composition of the present invention may contain physiologically active substances or minerals which are known in the art and which are stable as a food additive in addition to the above-mentioned food additives in order to supplement and supplement functional and nutritional properties.
그러한 생리활성 물질로서는 녹차 등에 포함된 카테킨류, 비타민 B1, 비타민 C, 비타민 E, 비타민 B12 등의 비타민류, 토코페롤, 디벤조일티아민 등을 들 수 있으며, 미네랄류로서는 구연산칼슘 등의 칼슘 제제, 스테아린산마그네슘 등의 마그네슘 제제, 구연산철 등의 철 제제, 염화크롬, 요오드칼륨, 셀레늄, 게르마늄, 바나듐, 아연 등을 들 수 있다. Examples of such physiologically active substances include catechins contained in green tea and the like, vitamins such as vitamin B1, vitamin C, vitamin E and vitamin B12, tocopherol, dibenzoyl thiamine, etc. Examples of minerals include calcium preparations such as calcium citrate, magnesium stearate , Iron preparations such as iron citrate, chromium chloride, potassium iodide, selenium, germanium, vanadium, zinc and the like.
본 발명의 식품 조성물에는 전술한 바의 식품첨가물이 제품 유형에 따라 그 첨가 목적을 달성할 수 있는 적량으로 포함될 수 있다.The food composition of the present invention may contain an appropriate amount of the above-mentioned food additives according to the product type so as to achieve the purpose of addition thereof.
본 발명의 식품 조성물에 포함될 수 있는 기타의 식품첨가물과 관련하여서는 각국 식품공전이나 식품첨가물 공전을 참조할 수 있다.With regard to other food additives that may be included in the food composition of the present invention, reference may be made to the Food Code of the respective countries or the Food Additives Code.
본 발명의 조성물은 다른 구체적인 양태에 있어서는 약제학적 조성물로 파악될 수 있다.In another specific embodiment, the composition of the present invention can be identified as a pharmaceutical composition.
본 발명의 약제학적 조성물은 유효성분 이외에 약제학적으로 허용되는 담체를 포함하여 당업계에 공지된 통상의 방법으로 투여 경로에 따라 경구용 제형 또는 비경구용 제형으로 제조될 수 있다. 여기서 투여 경로는 국소 경로, 경구 경로, 정맥 내 경로, 근육 내 경로, 및 점막 조직을 통한 직접 흡수를 포함하는 임의의 적절한 경로일 수 있으며, 두 가지 이상의 경로를 조합하여 사용할 수도 있다. 두 가지 이상 경로의 조합의 예는 투여 경로에 따른 두 가지 이상의 제형의 약물이 조합된 경우로서 예컨대 1차로 어느 한 약물은 정맥 내 경로로 투여하고 2차로 다른 약물은 국소 경로로 투여하는 경우이다. The pharmaceutical composition of the present invention may be prepared into oral formulations or parenteral formulations according to the route of administration by conventional methods known in the art, including pharmaceutically acceptable carriers in addition to the active ingredient. Where the route of administration may be any suitable route including local routes, oral routes, intravenous routes, intramuscular routes, and direct absorption through mucosal tissues, and combinations of two or more routes may be used. An example of a combination of two or more routes is a combination of two or more formulations of the drug according to the route of administration, for example, one drug is administered intravenously and another drug is administered via a local route.
약학적으로 허용되는 담체는 투여 경로나 제형에 따라 당업계에 주지되어 있으며, 구체적으로는 "대한민국약전"을 포함한 각국의 약전을 참조할 수 있다. Pharmaceutically acceptable carriers are well known in the art depending on the route of administration and formulation, and specific reference may be made to the pharmacopoeia of each country, including the " Korean Pharmacopoeia ".
본 발명의 약제학적 조성물이 경구용 제형으로 제조될 경우, 적합한 담체와 함께 당업계에 공지된 방법에 따라 분말, 과립, 정제, 환제, 당의정제, 캡슐제, 액제, 겔제, 시럽제, 현탁액, 웨이퍼 등의 제형으로 제조될 수 있다. 이때 적합한 담체의 예로서는 락토스, 글루코스, 슈크로스, 덱스트로스, 솔비톨, 만니톨, 자일리톨 등의 당류, 옥수수 전분, 감자 전분, 밀 전분 등의 전분류, 셀룰로오스, 메틸셀룰로오스, 에틸셀룰로오스, 나트륨 카르복시메틸셀룰로오스, 하이드록시프로필메틸셀룰로오스 등의 셀룰로오스류, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 마그네슘 스테아레이트, 광물유, 맥아, 젤라틴, 탈크, 폴리올, 식물성유, 에탄올, 그리세롤 등을 들 수 있다. 제제화활 경우 필요에 따라적절한 결합제, 윤활제, 붕해제, 착색제, 희석제 등을 포함시킬 수 있다. 적절한 결합제로서는 전분, 마그네슘 알루미늄 실리케이트, 전분페리스트, 젤라틴, 메틸셀룰로스, 소듐 카복시메틸셀룰로스, 폴리비닐피롤리돈, 글루코스, 옥수수 감미제, 소듐 알지네이트, 폴리에틸렌 글리콜, 왁스 등을 들 수 있고, 윤활제로서는 올레산나트륨, 스테아르산나트륨, 스테아르산마그네슘, 벤조산나트륨, 초산나트륨, 염화나트륨, 실리카, 탈쿰, 스테아르산, 그것의 마그네슘염과 칼슘염, 폴리데틸렌글리콜 등을 들 수 있으며, 붕해제로서는 전분, 메틸 셀룰로스, 아가(agar), 벤토나이트, 잔탄 검, 전분, 알긴산 또는 그것의 소듐 염 등을 들 수 있다. 또 희석제로서는 락토즈, 덱스트로즈, 수크로즈, 만니톨, 소비톨, 셀룰로스, 글라이신 등을 들 수 있다. When the pharmaceutical composition of the present invention is prepared into an oral formulation, it may be formulated into powder, granules, tablets, pills, sugar tablets, capsules, solutions, gels, syrups, suspensions, wafers And the like. Examples of suitable carriers include starches such as lactose, glucose, sucrose, dextrose, sorbitol, mannitol and xylitol, corn starch, potato starch and wheat starch, cellulose, methylcellulose, ethylcellulose, sodium carboxymethylcellulose, Hydroxypropylmethylcellulose and the like; polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, magnesium stearate, mineral oil, malt, gelatin, talc, polyol, vegetable oil, ethanol Serol, and the like. In case of formulation, suitable binders, lubricants, disintegrants, coloring agents, diluents and the like may be included as needed. Examples of suitable binders include starch, magnesium aluminum silicate, starch pellets, gelatin, methyl cellulose, sodium carboxymethyl cellulose, polyvinyl pyrrolidone, glucose, corn sweetener, sodium alginate, polyethylene glycol, wax and the like. Examples of the disintegrating agent include starch, methylcellulose, magnesium stearate, magnesium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, silica, talcum, stearic acid, Agar, bentonite, xanthan gum, starch, alginic acid or its sodium salt, and the like. Examples of the diluent include lactose, dextrose, sucrose, mannitol, sorbitol, cellulose, glycine and the like.
본 발명의 약제학적 조성물이 비경구용 제형으로 제조될 경우, 적합한 담체와 함께 당업계에 공지된 방법에 따라 주사제, 경피 투여제, 비강 흡입제 및 좌제의 형태로 제제화될 수 있다. 주사제로 제제화할 경우 적합한 담체로서는 수성 등장 용액 또는 현탁액을 사용할 수 있으며, 구체적으로는 트리에탄올 아민이 함유된 PBS(phosphate buffered saline)나 주사용 멸균수, 5% 덱스트로스 같은 등장 용액 등을 사용할 수 있다. 경피 투여제로 제제화할 경우 연고제, 크림제, 로션제, 겔제, 외용액제, 파스타제, 리니멘트제, 에어롤제 등의 형태로 제제화할 수 있다. 비강 흡입제의 경우 디클로로플루오로메탄, 트리클로로플루오로메탄, 디클로로테트라플루오로에탄, 이산화탄소 등의 적합한 추진제를 사용하여 에어로졸 스프레이 형태로 제제화할 수 있으며, 좌제로 제제화할 경우 그 담체로는 위텝솔(witepsol), 트윈(tween) 61, 폴리에틸렌글리콜류, 카카오지, 라우린지, 폴리옥시에틸렌 소르비탄 지방산 에스테르류, 폴리옥시에틸렌 스테아레이트류, 소르비탄 지방산 에스테르류 등을 사용할 수 있다.When the pharmaceutical composition of the present invention is prepared into a parenteral dosage form, it may be formulated in the form of an injection, transdermal drug delivery, nasal aspirate and suppository together with a suitable carrier according to methods known in the art. As the carrier suitable for injection preparation, aqueous isotonic solutions or suspensions may be used. Specifically, PBS (phosphate buffered saline) containing triethanolamine, sterile water for injection, and isotonic solution such as 5% dextrose may be used . When formulated with a transdermal preparation, it can be formulated in the form of ointments, creams, lotions, gels, external liquids, pastes, liniments, and air-lozenges. Nasal inhalers may be formulated in the form of aerosol sprays using suitable propellants such as dichlorofluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide, etc., and when formulated as a suppository, witepsol, tween 61, polyethylene glycols, cacao butter, laurin, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene stearates, and sorbitan fatty acid esters.
약제학적 조성물의 구체적인 제제화와 관련하여서는 당업계에 공지되어 있으며, 예컨대 문헌[Remington's Pharmaceutical Sciences(19th ed., 1995)] 등을 참조할 수 있다. 상기 문헌은 본 명세서의 일부로서 간주 된다.The formulation of pharmaceutical compositions is well known in the art and can be found, for example, in Remington ' s Pharmaceutical Sciences (19th ed., 1995). This document is considered part of this specification.
본 발명의 약제학적 조성물의 바람직한 투여량은 환자의 상태, 체중, 성별, 연령, 환자의 중증도, 투여 경로에 따라 1일 0.001mg/kg ~ 10g/kg 범위, 바람직하게는 0.001mg/kg ~ 1g/kg 범위일 수 있다. 투여는 1일 1회 또는 수회로 나누어 이루어질 수 있다. 이러한 투여량은 어떠한 측면으로든 본 발명의 범위를 제한하는 것으로 해석되어서는 아니 된다. The preferred dosage of the pharmaceutical composition of the present invention is 0.001 mg / kg to 10 g / kg per day, preferably 0.001 mg / kg to 1 g / day, depending on the patient's condition, body weight, sex, age, / kg < / RTI > The administration can be carried out once or several times a day. Such dosages should in no way be construed as limiting the scope of the invention.
전술한 바와 같이, 본 발명에 따르면 오미자 추출물과 칠해목 추출물을 이용한 뇌신경세포 보호용 조성물을 제공할 수 있으며, 이러한 뇌신경세포 보호용 조성물은 식품 또는 약품으로 제품화될 수 있다.As described above, according to the present invention, it is possible to provide a composition for protecting a brain cell using an extract of Omija and a chestnut tree extract, and the composition for protecting a brain cell can be commercialized as a food or a medicine.
도 1 및 도 2는 뇌신경세포의 세포독성 실험 결과이다.
도 3 내지 5는 뇌신경세포의 보호 활성 실험 결과이다.FIGS. 1 and 2 show the cytotoxicity test results of brain nerve cells.
Figs. 3 to 5 show the results of the protective activity test of neuronal cells.
이하 본 발명을 실시예 및 실험예를 참조하여 설명한다. 그러나 본 발명의 범위가 이러한 실시예 및 실험예에 한정되는 것은 아니다.Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these examples and experimental examples.
<실시예> <Examples> 오미자 추출물과 칠해목 추출물의 제조Preparation of Omiza extract and Pine wood extract
오미자 열매 및 칠해목 줄기와 잎 건조 분쇄물 각각 600g을 70% 에탄올에 1일간 냉침시킨후 여과하고 여액을 감압농축, 동결건조하였으며, 이 과정을 2회 반복하여 분말상의 오미자 추출물과 칠해목 추출물각각을 제조하였다.600 g of each of the oatmeal, pine needle stalks and leaf dry crumbs were frozen in 70% ethanol for 1 day, filtered, and the filtrate was concentrated under reduced pressure and lyophilized. This procedure was repeated twice to obtain powdered omija .
<실험예> <Experimental Example> 뇌신경세포 보호 활성 실험Experiment of neuronal cell protection activity
<실험예 1> <Experimental Example 1> 인간신경모세포종 SH-SY5Y 세포주에 세포독성 실험Cytotoxicity test on human neuroblastoma SH-SY5Y cell line
SH-SY5Y세포는 10% (v/v) 소태아혈청(fetal bovine serum, FBS), 1% 페니실린/스트렙토마이신(penicillin/streptomycin), NaHCO3 (2 mg/ml) 및 15 mM 헤페스(hepes)가 포함된 동물세포배양 배지(DMEM 배지)에 5% CO2 배양기 내에서 37℃에서 배양 하였다. 배양된 SH-SY5Y 세포를 96 웰 플레이트에 5×104세포/웰(cell/well)을 시딩(seeding)한 후, 시료를 농도별(10, 20 ㎍/ml)로 처리하고 37℃에서 24 시간 동안 배양하였다. 뇌신경세포 생존율은 MTT방법으로 측정하였는데, MTT법은 세포의 미토콘드리아 내 효소인 succinate-dehydrogenase에 의해 MTT가 formazan으로 변하여 죽은 세포는 formazan생성을 줄어 들게 하는 원리를 이용하여 흡광도를 측정하는 것이다. 구체적으로 96-well plate에 최종농도가 0.5 ㎎/㎖이 되도록 MTT (Sigma-Aldrich, cat.# M2128, USA) 용액을 각 well에 넣었다. 배양기에서 3시간 동안 반응시키고 배지와 MTT 용액을 제거한 후 DMSO를 넣어 교반하였다. 완전히 용해되었을 때 마이크로리더를 이용하여 540 ㎚에서 UV 흡광도를 측정하였다. 측정된 흡광도를 이용하여 세포생존율(%)을 시료 무처리군 대한 백분율로 도 1 및 도 2에 나타내었다. SH-SY5Y cells were cultured in the presence of 10% (v / v) fetal bovine serum (FBS), 1% penicillin / streptomycin, NaHCO 3 (2 mg / ml) and 15 mM hepes ) In an animal cell culture medium (DMEM medium) at 37 ° C in a 5% CO 2 incubator. The cultured SH-SY5Y cells were seeded at a density of 5 × 10 4 cells / well in a 96-well plate, treated with a concentration (10, 20 μg / ml) Lt; / RTI > Neuronal cell viability was measured by the MTT method. The MTT method measures absorbance using the principle that MTT is changed to formazan by succinate-dehydrogenase, which is an enzyme in mitochondria of cells, and dead cells reduce formazan production. Specifically, MTT (Sigma-Aldrich, Cat. # M2128, USA) solution was added to each well to a final concentration of 0.5 mg / ml on a 96-well plate. After incubation for 3 hours in the incubator, the medium and MTT solution were removed, and DMSO was added and stirred. When fully dissolved, the UV absorbance was measured at 540 nm using a micro-reader. The cell viability (%) using the measured absorbance is shown in Figures 1 and 2 as a percentage of the untreated group.
도 1을 참조하여 보면, 실시예의 각 추출물은 모두 10, 20 ㎍/ml의 처리 농도에서 특별한 세포독성을 보이지 않았다.Referring to FIG. 1, each of the extracts of the Examples showed no specific cytotoxicity at a treatment concentration of 10, 20 占 퐂 / ml.
또 도 2를 참조하여 보면 실시예의 오미자 추출물과 칠해목 추출물의 6:4, 7:3 및 8:2 중량비 혼합물도 모두 10, 20 ㎍/ml의 처리 농도에서 특별한 세포독성을 보이지 않았다.Also, referring to FIG. 2, the mixture of 6: 4, 7: 3, and 8: 2 by weight of the extracts of Omiza extract and Pyrrhizae extract did not show any cytotoxicity at the treatment concentrations of 10 and 20 μg / ml.
<실험예 2> <Experimental Example 2> 추출물 단독의 인간신경모세포종 SH-SY5Y 세포의 보호 활성The protective activity of human neuroblastoma SH-SY5Y cells alone
배양된 SH-SY5Y 세포를 96 웰 플레이트에 5×104세포/웰(cell/well)을 시딩(seeding)한 후 37℃에서 24 시간 동안 배양하였다. 그 후 시료를 농도별(10, 20 ㎍/ml)로 처리하고 37℃에서 24 시간 동안 배양한 다음, 뇌신경세포의 사멸을 유도하기 위해 과산화수소 100uM을 1시간 처리하여 약 50%의 세포사멸을 유도 하였다. 다음 실시예 1과 동일한 MTT Assay를 통해 세포생존율을 과산화수소만의 처리군 대비 세포생존율을 구하여 도 3 내 도 5에 나타내었다. The cultured SH-SY5Y cells were seeded at 5 × 10 4 cells / well in a 96-well plate and cultured at 37 ° C. for 24 hours. After incubation at 37 ° C for 24 hours, the cells were treated with 100 μM hydrogen peroxide for 1 hour to induce apoptosis of neurons. Approximately 50% cell death was induced Respectively. The cell survival rate of the cells treated with hydrogen peroxide alone was determined by the same MTT assay as in Example 1 and shown in FIG.
도 3을 참조하여 보면 실시예의 각 추출물은 모두 농도 의존적으로 과산화수소에 의한 신경세포의사멸을 억제함을 보여준다.Referring to FIG. 3, each of the extracts of the Examples showed that the inhibition of peroxide-induced neuronal cell death was suppressed in a concentration-dependent manner.
또 도 4를 참조하여 보면, 실시예의 오미자 추출물과 칠해목 추출물의 혼합물도 모두 과산화수소에 의한 신경세포의사멸을 억제함을 보여준다. 특히 6:4 중량비의 혼합물의 활성이 가장 우수하였다. Also, referring to FIG. 4, it can be seen that both the mixture of Omiza extract and Pyrrhizae extract in Example suppresses the death of nerve cells by hydrogen peroxide. In particular, the activity of the 6: 4 weight ratio mixture was the most excellent.
도 5에는 오미자 추출물 20 ㎍/ml, 칠해목 추출물 20 ㎍/ml 및 오미자 추출물과 칠해목 추출물의 혼합물 10 ㎍/ml의 뇌신경세포 보호 활성을 함께 나타내었는데, 오미자 추출물과 칠해목 추출물의 혼합물이 10 ㎍/ml의 농도에서 가장 높은 것으로 나타났다.FIG. 5 also shows the cranial nerve cell protection activity of 20 μg / ml of Omija extract, 20 μg / ml of Pyrrhizae extract, and 10 μg / ml of a mixture of Omiza extract and Pyrrhizae extract. ㎍ / ml.
Claims (13)
A composition for protecting a brain cell comprising an extract of Ornithia, an extract of Pine wood, or a mixture thereof as an active ingredient.
상기 추출물은 물, 에탄올 또는 이들의 혼합 용매로 추출하여 얻은 추출물인 것을 특징으로 하는 조성물.
The method according to claim 1,
Wherein the extract is an extract obtained by extracting with water, ethanol or a mixed solvent thereof.
상기 조성물은 약제학적 조성물인 것을 특징으로 하는 조성물.
3. The method according to claim 1 or 2,
Wherein the composition is a pharmaceutical composition.
상기 조성물은 식품 조성물인 것을 특징으로 하는 조성물.
3. The method according to claim 1 or 2,
Wherein the composition is a food composition.
Composition for improving diseases accompanied by death of brain cells comprising an extract of Ornithia, extract of Pine wood, or a mixture thereof as an active ingredient.
상기 추출물은 물, 에탄올 또는 이들의 혼합 용매로 추출하여 얻은 추출물인 것을 특징으로 하는 조성물.
6. The method of claim 5,
Wherein the extract is an extract obtained by extracting with water, ethanol or a mixed solvent thereof.
상기 뇌신경세포의 사멸을 수반하는 질환은 퇴행성 뇌질환 또는 허혈성 뇌질환인 조성물.
The method according to claim 1,
Wherein said disease involving the death of said neuronal cell is a degenerative brain disease or ischemic brain disease.
상기 조성물은 약제학적 조성물인 것을 특징으로 하는 조성물.
The method according to claim 5 or 6,
Wherein the composition is a pharmaceutical composition.
상기 조성물은 식품 조성물인 것을 특징으로 하는 조성물.
The method according to claim 5 or 6,
Wherein the composition is a food composition.
Composition for improving cognitive ability comprising as an active ingredient an extract of Ornithia, an extract of Pine wood, or a mixture thereof.
상기 추출물은 물, 에탄올 또는 이들의 혼합 용매로 추출하여 얻은 추출물인 것을 특징으로 하는 조성물.
11. The method of claim 10,
Wherein the extract is an extract obtained by extracting with water, ethanol or a mixed solvent thereof.
상기 조성물은 약제학적 조성물인 것을 특징으로 하는 조성물.
The method according to claim 10 or 11,
Wherein the composition is a pharmaceutical composition.
상기 조성물은 식품 조성물인 것을 특징으로 하는 조성물.
The method according to claim 10 or 11,
Wherein the composition is a food composition.
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KR20200060685A (en) * | 2018-11-22 | 2020-06-01 | 재단법인 경기도경제과학진흥원 | Simultaneous Analytical Method of Marker Compounds in a Mixed Extract of Schizandra chinensis and Ribes fasciculatum |
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Citations (2)
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KR20160149853A (en) * | 2015-06-19 | 2016-12-28 | 부산대학교 산학협력단 | Composition for treating, improving or preventing neurodegenerative diseases |
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KR20200060685A (en) * | 2018-11-22 | 2020-06-01 | 재단법인 경기도경제과학진흥원 | Simultaneous Analytical Method of Marker Compounds in a Mixed Extract of Schizandra chinensis and Ribes fasciculatum |
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