KR102327092B1 - Cosmetic composition having mauritia flexuosa fruit oil - Google Patents

Cosmetic composition having mauritia flexuosa fruit oil Download PDF

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KR102327092B1
KR102327092B1 KR1020210057182A KR20210057182A KR102327092B1 KR 102327092 B1 KR102327092 B1 KR 102327092B1 KR 1020210057182 A KR1020210057182 A KR 1020210057182A KR 20210057182 A KR20210057182 A KR 20210057182A KR 102327092 B1 KR102327092 B1 KR 102327092B1
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oil
cosmetic composition
aguahe
extract
weight
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Korean (ko)
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김현우
정혜선
박시은
이홍
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주식회사 네이처앤네이처
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/02Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04Dispersions; Emulsions
    • A61K8/06Emulsions
    • A61K8/062Oil-in-water emulsions
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9789Magnoliopsida [dicotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74Biological properties of particular ingredients
    • A61K2800/78Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782Enzyme inhibitors; Enzyme antagonists

Abstract

The present invention relates to a cosmetic composition and, more specifically, to a cosmetic composition comprising Mauritia flexuosa oil as an active ingredient. The cosmetic composition according to the present invention has an advantage in that it exhibits excellent moisturizing and anti-inflammatory effects by comprising Mauritia flexuosa oil as an active ingredient. In addition, the cosmetic composition has an advantage in that it can satisfy consumers' demand for a cosmetic composition derived from a natural product that is environmentally friendly and has fewer side effects.

Description

아구아헤 오일을 포함하는 화장료 조성물{COSMETIC COMPOSITION HAVING MAURITIA FLEXUOSA FRUIT OIL}Cosmetic composition comprising aguahe oil {COSMETIC COMPOSITION HAVING MAURITIA FLEXUOSA FRUIT OIL}

본 발명은 화장료 조성물에 관한 것으로, 더욱 상세하게는 아구아헤 오일을 화장료 조성물 내 유효성분으로 포함함으로써, 우수한 보습 및 항염증 효과를 부유하는 화장료 조성물에 관한 것이다. The present invention relates to a cosmetic composition, and more particularly, to a cosmetic composition containing aguahe oil as an active ingredient in the cosmetic composition, thereby providing excellent moisturizing and anti-inflammatory effects.

피부는 외부에서부터 순서대로 표피, 진피, 피하지방 조직의 3개 층으로 크게 구분되며, 인체를 외부 환경의 물리적, 화학적인 자극으로부터 보호해 주는 기능을 하는데, 특히 피부는 인체가 갖고 있는 약 65~70%의 수분이 체외로 증발되는 것을 조절해 준다.The skin is largely divided into three layers: the epidermis, the dermis, and the subcutaneous fat tissue in order from the outside. It controls the evaporation of 70% of water out of the body.

그 중에서도 표피는 외부로부터 각질층, 과립층, 유극층, 기저층의 순서로 구분되고, 표피의 각질층은 약 10~20%의 수분을 함유하며 인체의 최외각에 존재하여 체내로부터의 수분 증발을 억제하는 한편 외부로부터의 물질 과잉 침투를 차단한다(J. Invest. Dermatol. 80(Suppl.), 44-49. 1983). Among them, the epidermis is divided from the outside into the stratum corneum, granular layer, stratum corneum, and basal layer. Blocks excessive penetration of substances from the outside ( J. Invest. Dermatol. 80 (Suppl.), 44-49. 1983 ).

이러한 각질층을 구성하고 있는 세포에는 수용성 성분인 고농도의 자연보습인자(Natural Moisturizing Factor, NMF)가 존재하여 피부가 유연성을 나타내도록 작용함은 물론 적절한 수분을 유지할 수 있도록 돕는다(J. Invest. Dermatol. 54, 24-31, 1970). A high concentration of Natural Moisturizing Factor (NMF), a water-soluble component, exists in the cells constituting the stratum corneum, which helps the skin to show flexibility and maintain adequate moisture ( J. Invest. Dermatol. 54, 24-31, 1970 ).

그러나 환경의 변화 혹은 생활 패턴의 변화, 사회생활에서 발생하는 각종 스트레스와 환경오염, 화장 습관에 따른 잦은 세안 및 연령 증가에 따른 자연적인 피부 노화 등의 원인으로 각질층의 수분이 감소하여 피부가 건조해지고 표면이 거칠게 되며, 피부가 윤기를 잃어 칙칙하게 보이는 등의 현상이 발생하여 피부 보습에 관한 문제는 그 중요성이 점점 더 커지고 있다. However, due to changes in the environment or lifestyle, various stresses and environmental pollution in social life, frequent washing according to makeup habits, and natural skin aging due to increasing age, the moisture in the stratum corneum decreases and the skin becomes dry. As the surface becomes rough and the skin loses its luster and looks dull, the problem of skin moisturizing is becoming more and more important.

이에, 최근에는 피부 보습을 개선하고자 하는 의미에서 다양한 화장품 원료들이 개발되고 있는데, 근래의 외부 환경을 살펴 보면 동물성 원료들에 대한 소비자들의 선호도 하락과 광우병 등의 파문으로 동물성 원료에 대한 사용이 급격히 줄어들고 천연 식물 유래의 원료들이 각광 받고 있다. Accordingly, in recent years, various cosmetic raw materials are being developed in the sense of improving skin moisture. Looking at the recent external environment, the use of animal raw materials has drastically decreased due to the decline in consumer preference for animal raw materials and the ripple of mad cow disease. Raw materials derived from natural plants are in the spotlight.

이러한 식물성 원료로는 더위지기, 병풀, 쌀배아 추출물 등이 개발된 바 있다.As such botanical raw materials, heat radish, centella asiatica, and rice germ extract have been developed.

한편, 아구아헤는 이테야자라는 야자나무의 열매로, 아마존 원주민들은 건강에 좋다는 이유로 생명의 나무라고 부르기도 했다. 남미 열대 지방의 늪지나 습한 지역에서 잘 자라며, 겉은 거북이 등껍질처럼 생겼지만, 껍질을 벗기면 노란 과육이 나온다.On the other hand, aguahe is the fruit of a palm tree called ite palm, which Native Americans called the tree of life because it is good for health. It grows well in swamps and wet areas of tropical South America. It has a tortoise shell-like appearance, but when peeled, yellow flesh emerges.

이러한 아구아헤는 식물성 에스트로겐을 다량 함유해 중년 여성의 갱년기 증상을 개선하는데 좋으며, 아구아헤 오일의 경우 필수 지방산, 비타민 A, 비타민 C, 비타민 E, 베타카로틴 등의 다양한 영양소를 다량 포함한다.Aguahe oil contains a large amount of phytoestrogens and is good for improving menopausal symptoms in middle-aged women. In the case of aguahe oil, it contains a large amount of various nutrients such as essential fatty acids, vitamin A, vitamin C, vitamin E, and beta-carotene.

상기 아구아헤는 주로 식품으로 섭취하여 왔으나, 보습 및 항염증용 화장료 조성물로서 사용된 바 없다.The aguahe has been mainly consumed as food, but has not been used as a cosmetic composition for moisturizing and anti-inflammatory.

KR 10-1198129 B1KR 10-1198129 B1 KR 10-2021-0021209 AKR 10-2021-0021209 A KR 10-2195365 B1KR 10-2195365 B1

본 발명의 목적은 아구아헤 오일을 포함하는 피부 보습용 화장료 조성물을 제공하는 데 있다.An object of the present invention is to provide a cosmetic composition for moisturizing the skin comprising aguahe oil.

본 발명의 다른 목적은 아구아헤 오일을 포함하는 항염증용 화장료 조성물을 제공하는 데 있다.Another object of the present invention is to provide an anti-inflammatory cosmetic composition comprising aguahe oil.

상기한 목적을 달성하기 위한 본 발명에 따른 아구아헤 오일을 포함하는 화장료 조성물은, 아구아헤 오일을 유효성분으로 포함하는 것을 특징으로 한다.A cosmetic composition comprising aguahe oil according to the present invention for achieving the above object is characterized in that it comprises aguahe oil as an active ingredient.

상기 화장료 조성물은 수중유형 에멀젼(O/W emulsion)으로, 수상, 유상 및 실리콘상이 혼합된 것이며, 상기 수상은 디소듐이디티에이(Disodium EDTA), 글리세린(Glycerin), 프로판다이올(propanediol), 1,2-헥산다이올(1,2-Hexanediol), 디스테아레스-100 IPDI(disteareth-100 IPDI), 암모늄아크릴로일디메칠타우레이트/브이피코폴리머(Ammonium Acryloyldimethyltaurate/VP Copolymer) 및 정제수를 포함하고, 상기 유상은 상기 아구아헤 오일, 부틸렌글라이콜다이카프릴레이트/다이카프레이트(Butylene Glycol Dicaprylate/Dicaprate), 다이-C12-13 알킬말레이트(DI-C12-13 Alkyl Malate) 및 하이드로제네이티드폴리(C6-14올레핀)(Hydrogenated Poly(C6-14 Olefin))를 포함하며, 상기 실리콘상은 페닐트리메치콘(Phenyl Trimethicone), 사이클로펜타실록세인(Cyclopentasiloxane), 다이메티콘/비닐다이메티콘크로스폴리머(Dimethicone/Vinyl Dimethicone Crosspolymer) 및 폴리메틸실세스퀴옥세인(Polymethylsilsesquioxane)을 포함하는 것을 특징으로 한다.The cosmetic composition is an oil-in-water emulsion (O/W emulsion), in which an aqueous phase, an oil phase and a silicone phase are mixed, and the aqueous phase is Disodium EDTA, glycerin (Glycerin), propanediol, Contains 1,2-hexanediol (1,2-Hexanediol), disteareth-100 IPDI (disteareth-100 IPDI), Ammonium Acryloyldimethyltaurate/VP Copolymer (Ammonium Acryloyldimethyltaurate/VP Copolymer) and purified water And, the oil phase is the aguahe oil, butylene glycol dicaprylate / dicaprate (Butylene Glycol Dicaprylate / Dicaprate), di-C12-13 alkyl malate (DI-C12-13 Alkyl Malate) and Hydrogen Hydrogenated Poly(C6-14 Olefin), wherein the silicone phase is Phenyl Trimethicone, Cyclopentasiloxane, Dimethicone/Vinyldimethicone It is characterized in that it contains a cross polymer (Dimethicone / Vinyl Dimethicone Crosspolymer) and polymethylsilsesquioxane (Polymethylsilsesquioxane).

상기 화장료 조성물 100중량%에 대하여, 상기 아구아헤 오일을 0.1~10중량%만큼 포함하며, 보습 및 항염증용인 것을 특징으로 한다.Based on 100% by weight of the cosmetic composition, 0.1 to 10% by weight of the aguahe oil is included, and it is characterized in that it is for moisturizing and anti-inflammatory.

후피향나무 추출물 0.1~5중량% 및 계수나무껍질 추출물 0.1~5중량%를 더 포함하며, 엘라스타제 저해 활성 및 타이로시나아제 저해 활성을 갖는 것을 특징으로 한다.It is characterized in that it further comprises 0.1 to 5% by weight of the fenugreek extract and 0.1 to 5% by weight of the cassia bark extract, and has elastase inhibitory activity and tyrosinase inhibitory activity.

본 발명에 의하면, 아구아헤 오일을 화장료 조성물의 유효성분으로 포함함으로써, 우수한 보습 효과 및 항염증 효과를 나타낸다는 장점이 있다. 또한, 자연친화적이면서 부작용이 적은 천연물 유래의 화장료 조성물에 대한 소비자들의 수요를 충족시킬 수 있는 장점이 있다.According to the present invention, by including aguahe oil as an active ingredient in the cosmetic composition, there is an advantage in that it exhibits excellent moisturizing effect and anti-inflammatory effect. In addition, there is an advantage that can satisfy the demand of consumers for a cosmetic composition derived from a natural product that is environmentally friendly and has fewer side effects.

도 1은 시험예 2의 항염증 효능을 실험한 결과를 나타낸 그래프.1 is a graph showing the results of testing the anti-inflammatory efficacy of Test Example 2.

이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.

본 발명에 따른 화장료 조성물은, 아구아헤 오일(MAURITIA FLEXUOSA FRUIT OIL)을 유효성분으로 포함하는 것을 특징으로 한다. 즉, 본 발명은 상기 아구아헤 오일을 통해 보습 및 항염증 기능을 나타냄으로써, 피부염증, 건조증 등의 피부개선 효과를 가진다. 또한, 이러한 아구아헤 오일은 식물 원료로 피부에 자극이 없으며, 안정성 또한 우수하여 천연물 유래의 화장료 조성물로서 적합하다는 특징이 있다.The cosmetic composition according to the present invention is characterized in that it contains MAURITIA FLEXUOSA FRUIT OIL as an active ingredient. That is, the present invention exhibits moisturizing and anti-inflammatory functions through the aguahe oil, thereby having skin improvement effects such as skin inflammation and dryness. In addition, the aguahe oil is a plant raw material and has no irritation to the skin, and has excellent stability, so that it is suitable as a cosmetic composition derived from a natural product.

본 발명에서 상기 아구아헤 오일은 화장료 조성물 100중량%에 대하여 0.1~10중량%로 포함됨이 바람직한데, 그 함량이 너무 적을 경우 보습 및 항염증 효과가 미미하고, 그 함량이 더 높을 경우 추가적인 보습 및 항염증 효과가 나타나지 않으면서도 제조비용이 상승하고, 제형 안정성이 떨어지는 등의 단점이 있기 때문이다.In the present invention, the aguahe oil is preferably included in an amount of 0.1 to 10% by weight based on 100% by weight of the cosmetic composition. When the content is too small, the moisturizing and anti-inflammatory effects are insignificant, and when the content is higher, additional moisturizing and This is because there are disadvantages such as an increase in manufacturing cost and poor formulation stability without showing an anti-inflammatory effect.

본 발명에서 상기 아구아헤 오일은 이테야자열매(MAURITIA FLEXUOSA FRUIT)로부터 추출한 것이라면 그 추출방법과 무관하게 모두 사용할 수 있는 것으로, 그 추출방법을 제한하지 않음은 당연하다. 또한, 시판 상품 중 어떠한 것이라도 구입하여 사용하는 것이 가능하다. In the present invention, as long as the aguahe oil is extracted from MAURITIA FLEXUOSA FRUIT, it can be used regardless of the extraction method, and it is natural that the extraction method is not limited. In addition, it is possible to purchase and use any of the commercially available products.

본 발명의 화장료 조성물은 상기 유효성분 이외에 통상적으로 허용되는 성분들을 제한 없이 포함할 수 있는바, 예컨데 희석제, 부형제, 완충제, 분산제, 계면 활성제, 착색제, 향료 또는 감미제 등의 통상적인 보조제를 포함할 수 있다. 아울러, 상기 화장료 조성물은 피부에 미용 기능성을 부여하는 기능성 화장 성분, 보습제, 기타 첨가제를 더 첨가할 수도 있으며, 일반 피부 화장료에 배합되는 화장품학적으로 허용 가능한 담체를 1종 이상 추가로 포함할 수도 있다. 이러한 담체 성분으로는 동물성 유, 식물성 유, 왁스, 파라핀, 실리콘, 락토스, 실리카, 탈크, 물, 에탄올, 벤질 알콜, 글리세롤 지방족 에스테르, 지방족 알코올 설페이트 등이 사용될 수 있는바, 그 종류를 한정하지 않음은 당연하다.The cosmetic composition of the present invention may include, without limitation, conventionally acceptable ingredients in addition to the active ingredients, for example, diluents, excipients, buffers, dispersants, surfactants, colorants, flavorings, or sweeteners, such as conventional adjuvants. have. In addition, the cosmetic composition may further add a functional cosmetic ingredient that imparts cosmetic functionality to the skin, a moisturizer, and other additives, and may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics. . Animal oil, vegetable oil, wax, paraffin, silicone, lactose, silica, talc, water, ethanol, benzyl alcohol, glycerol aliphatic ester, aliphatic alcohol sulfate, etc. may be used as the carrier component, but the type is not limited. is natural

또한, 본 발명에 따른 화장료 조성물은 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클린싱, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이로 이루어진 군으로부터 선택되는 어느 하나의 제형을 가질 수 있으며, 이에 제한되는 것은 아니다.In addition, the cosmetic composition according to the present invention is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, and a spray group consisting of It may have any one formulation selected from, but is not limited thereto.

본 발명의 화장료 조성물은 가장 바람직하게, 수중유형 에멀젼(O/W emulsion)으로 제조되는 것인바, 이는 아구아헤 오일의 함량을 높여 보습 및 항염증 활성은 높이면서도, 끈적이는 사용감을 낮출 수 있기 때문이다. The cosmetic composition of the present invention is most preferably prepared as an oil-in-water emulsion (O/W emulsion), which increases the content of aguahe oil to increase moisturizing and anti-inflammatory activity, while lowering the sticky feeling of use. .

상기 수중유형의 화장료 조성물은, 수상, 유상 및 실리콘상이 혼합된 것이며, 상기 수상은 디소듐이디티에이(Disodium EDTA), 글리세린(Glycerin), 프로판다이올(propanediol), 1,2-헥산다이올(1,2-Hexanediol), 디스테아레스-100 IPDI(disteareth-100 IPDI), 암모늄아크릴로일디메칠타우레이트/브이피코폴리머(Ammonium Acryloyldimethyltaurate/VP Copolymer) 및 정제수를 포함하고, 상기 유상은 상기 아구아헤 오일, 부틸렌글라이콜다이카프릴레이트/다이카프레이트(Butylene Glycol Dicaprylate/Dicaprate), 다이-C12-13 알킬말레이트(DI-C12-13 Alkyl Malate) 및 하이드로제네이티드폴리(C6-14올레핀)(Hydrogenated Poly(C6-14 Olefin))를 포함하며, 상기 실리콘상은 페닐트리메치콘(Phenyl Trimethicone), 사이클로펜타실록세인(Cyclopentasiloxane), 다이메티콘/비닐다이메티콘크로스폴리머(Dimethicone/Vinyl Dimethicone Crosspolymer) 및 폴리메틸실세스퀴옥세인(Polymethylsilsesquioxane)을 포함하는 것을 특징으로 한다.The oil-in-water cosmetic composition is a mixture of an aqueous phase, an oil phase and a silicone phase, and the aqueous phase is Disodium EDTA, glycerin, propanediol, 1,2-hexanediol (1,2-Hexanediol), disteareth-100 IPDI (disteareth-100 IPDI), ammonium acryloyldimethyltaurate/VP Copolymer (Ammonium Acryloyldimethyltaurate/VP Copolymer) and purified water, and the oil phase is the agua Hee Oil, Butylene Glycol Dicaprylate/Dicaprate, DI-C12-13 Alkyl Malate and Hydrogenated Poly(C6-14 Olefins) ) (Hydrogenated Poly(C6-14 Olefin)), and the silicone phase is Phenyl Trimethicone, Cyclopentasiloxane, Dimethicone/Vinyl Dimethicone Crosspolymer (Dimethicone/Vinyl Dimethicone) Crosspolymer) and polymethylsilsesquioxane (Polymethylsilsesquioxane).

먼저, 상기 수상은 금속이온봉쇄제로서 디소듐이디티에이(Disodium EDTA)를, 폴리올 보습성분으로 글리세린(Glycerin), 프로판다이올(propanediol), 1,2-헥산다이올(1,2-Hexanediol)을 함유하며, 경도형성제로 디스테아레스-100 IPDI(disteareth-100 IPDI)을, 점도증가제로 암모늄아크릴로일디메칠타우레이트/브이피코폴리머(Ammonium Acryloyldimethyltaurate/VP Copolymer)를 함유하는 것이다. First, the aqueous phase contains Disodium EDTA as a sequestering agent, and glycerin, propanediol, and 1,2-Hexanediol as polyol moisturizing ingredients. ), disteareth-100 IPDI as a hardness forming agent, and ammonium acryloyldimethyltaurate/VP Copolymer as a viscosity increasing agent.

상기 유상은 기능성 물질로 아구아헤 오일을, 피부컨디셔닝제로 부틸렌글라이콜다이카프릴레이트/다이카프레이트(Butylene Glycol Dicaprylate/Dicaprate), 다이-C12-13 알킬말레이트(DI-C12-13 Alkyl Malate), 하이드로제네이티드폴리(C6-14올레핀)(Hydrogenated Poly(C6-14 Olefin))를 함유하는 것이다. The oil phase uses aguahe oil as a functional material, butylene glycol dicaprylate/dicaprate as a skin conditioning agent, DI-C12-13 Alkyl Malate ), hydrogenated poly (C6-14 olefin) (Hydrogenated Poly (C6-14 Olefin)).

상기 실리콘상은 수분증발차단제로 페닐트리메치콘(Phenyl Trimethicone), 유연제로 사이클로펜타실록세인(Cyclopentasiloxane), 점도증가제로 다이메티콘/비닐다이메티콘크로스폴리머(Dimethicone/Vinyl Dimethicone Crosspolymer) 및 불투명화제로 폴리메틸실세스퀴옥세인(Polymethylsilsesquioxane)을 함유하는 것이다. The silicone phase contains Phenyl Trimethicone as a moisture evaporation blocking agent, Cyclopentasiloxane as a softening agent, Dimethicone/Vinyl Dimethicone Crosspolymer as a viscosity increasing agent, and Opacifying agent. It contains polymethylsilsesquioxane.

이때 그 배합비는, 디소듐이디티에이(Disodium EDTA) 0.01~1중량%, 글리세린(Glycerin) 0.1~40중량%, 프로판다이올(propanediol) 0.1~10중량%, 1,2-헥산다이올(1,2-Hexanediol) 0.1~1중량%, 디스테아레스-100 IPDI(disteareth-100 IPDI) 0.1~5중량%, 암모늄아크릴로일디메칠타우레이트/브이피코폴리머(Ammonium Acryloyldimethyltaurate/VP Copolymer) 0.1~1중량%, 아구아헤 오일 0.1~10중량%, 부틸렌글라이콜다이카프릴레이트/다이카프레이트(Butylene Glycol Dicaprylate/Dicaprate) 0.1~5중량%, 다이-C12-13 알킬말레이트(DI-C12-13 Alkyl Malate) 0.1~10중량%, 하이드로제네이티드폴리(C6-14올레핀)(Hydrogenated Poly(C6-14 Olefin)) 0.1~1중량%, 페닐트리메치콘(Phenyl Trimethicone) 1~6중량%, 사이클로펜타실록세인(Cyclopentasiloxane) 1~5중량%, 다이메티콘/비닐다이메티콘크로스폴리머(Dimethicone/Vinyl Dimethicone Crosspolymer) 0.1~5중량%, 폴리메틸실세스퀴옥세인(Polymethylsilsesquioxane) 0.1~5중량% 및 잔부의 정제수를 포함함이 바람직하나, 상기 아구아헤 오일을 제외하고는 그 배합비를 반드시 제한하는 것은 아니다.At this time, the mixing ratio is disodium EDTA 0.01 to 1% by weight, glycerin 0.1 to 40% by weight, propanediol 0.1 to 10% by weight, 1,2-hexanediol ( 1,2-Hexanediol) 0.1~1% by weight, disteareth-100 IPDI (disteareeth-100 IPDI) 0.1~5% by weight, Ammonium Acryloyldimethyltaurate/VP Copolymer (Ammonium Acryloyldimethyltaurate/VP Copolymer) 0.1~ 1 wt%, Aguahe oil 0.1-10 wt%, Butylene Glycol Dicaprylate/Dicaprate 0.1-5 wt%, Di-C12-13 Alkylmaleate (DI-C12) -13 Alkyl Malate) 0.1 to 10% by weight, Hydrogenated Poly(C6-14 Olefin) 0.1 to 1% by weight, Phenyl Trimethicone 1 to 6% by weight , Cyclopentasiloxane 1-5 wt%, Dimethicone/Vinyl Dimethicone Crosspolymer 0.1-5 wt%, Polymethylsilsesquioxane 0.1-5 wt% % and the remainder of the purified water is preferably included, but the blending ratio is not necessarily limited except for the aguahe oil.

그리고 상기 수중유형 에멀젼(O/W emulsion) 형태의 화장료 조성물을 제조하는 방법은 제한하지 않는바, 종래 게시된 수중유형 에멀젼을 제조하는 방법에 의하는 것이면 족하다. 예시적으로, 상기 정제수에 폴리올 보습성분을 용해시키고, 이에 금속이온봉쇄제, 점도증가제 및 경도형성제를 분산 용해시켜 점성의 수상(겔상)을 제조하고, 이에 유상 및 실리콘상을 첨가하여 호모믹서로 충분히 교반하여 유화시켜 제조하면 족하다. And the method for preparing the cosmetic composition in the form of the oil-in-water emulsion (O/W emulsion) is not limited, as long as it is according to the method for preparing the oil-in-water emulsion published in the prior art. Illustratively, a viscous aqueous phase (gel phase) is prepared by dissolving a polyol moisturizing component in the purified water, and dispersing and dissolving a sequestering agent, a viscosity increasing agent, and a hardness forming agent therein. It is sufficient to emulsify it by sufficiently stirring it with a mixer.

상기와 같은 본 발명의 화장료 조성물은 우수한 보습 효과 및 항염 활성을 갖는다는 장점이 있다.The cosmetic composition of the present invention as described above has the advantage of having an excellent moisturizing effect and anti-inflammatory activity.

한편, 본 발명의 화장료 조성물은 미백 및 항주름 효과를 위하여, 후피향나무 추출물 및 계수나무껍질 추출물을 더 포함함이 바람직하다. 즉, 상기 아구아헤 오일은 보습 및 항염증 효과는 우수하나, 미백 및 항주름 효과는 미흡한 바 이를 개선하기 위함이다. 이때, 상기 후피향나무 추출물 및 계수나무 추출물은 화장료 조성물 100중량%에 대하여, 각각 0.1~5중량%의 범위로 포함됨이 바람직하다. On the other hand, the cosmetic composition of the present invention for whitening and anti-wrinkle effect, it is preferable to further include an extract of fenugreek and cassia bark extract. That is, the aguahe oil has excellent moisturizing and anti-inflammatory effects, but has insufficient whitening and anti-wrinkle effects. At this time, it is preferable that the extract of fenugreek extract and cassia tree extract are included in the range of 0.1 to 5% by weight, respectively, based on 100% by weight of the cosmetic composition.

상기 후피향나무 추출물 및 계수나무 추출물은 우수한 엘라스타제 저해 활성 및 타이로시나아제 저해 활성을 갖는바, 이를 통해 미백 및 항주름 효과를 갖는 것이다. The fenugreek extract and cassia extract have excellent elastase inhibitory activity and tyrosinase inhibitory activity, thereby having whitening and anti-wrinkle effects.

후피향나무는 우리나라 남해안 제주도, 중국, 일본, 필리핀, 베트남 등에 자생하는 상록 활엽수로, 붉은 열매가 아름답고 잎이 윤택이 나며 온도나 채광에 따라 붉게 물들기도 하여 주로 정원수나 관상용 나무로 사용되어 왔다. 상기 후피향나무 추출물은, 후피향(Ternstroemia kwangtungensis)의 잎 추출물을 의미하는 것으로, 엘라스타제 저해 활성을 갖는다. Pachyderm is an evergreen broad-leaved tree that is native to Jeju Island, China, Japan, the Philippines, and Vietnam in the southern coast of Korea. It has beautiful red fruits and glossy leaves, and has been used mainly as a garden tree or ornamental tree. The extract of fenugreek extract, which refers to a leaf extract of fenugreek (Ternstroemia kwangtungensis), has elastase inhibitory activity.

계수나무(Cercidiphyllum Japonicum S et Z)는 쌍떡잎식물 미나리아재비목 계수나무과의 낙엽활엽교목으로, 그 추출물은 타이로시나아제 저해 활성을 갖는다. Cassia (Cercidiphyllum Japonicum S et Z) is a deciduous broad-leaved tree of the dicotyledonous plant Heterinus Cassiaceae, and its extract has tyrosinase inhibitory activity.

이때, 상기 추출물의 추출방법은 제한하지 않는바, 후피향나무의 잎 또는 계수나무 껍질을 시료로 하고, 물, 메탄올(methanol), 에탄올(ethanol), 프로판올(propanol), 이소프로판올(isopropanol), 부탄올(butanol) 등을 포함하는 탄소수 1 내지 4의 알코올 중 1종 이상을 용매로 하여 열수추출법, 냉침추출법, 환류냉각추출법, 용매추출법, 수증기증류법, 초음파추출법, 용출법, 압착법 중 1종의 방법으로 추출할 수 있다. 또한, 목적하는 추출물은 추가로 통상의 분획 공정을 수행할 수도 있으며, 통상의 정제 방법을 이용하여 정제될 수도 있고, 희석, 농축 또는 건조될 수도 있는 것으로, 그 실시를 제한하지 않는다.At this time, the extraction method of the extract is not limited, and the leaves or cassia bark of the H. japonica are used as samples, and water, methanol, ethanol, propanol, isopropanol, butanol ( butanol), using one or more of the alcohols having 1 to 4 carbon atoms as a solvent, and using one of hot water extraction method, cold extraction method, reflux cooling extraction method, solvent extraction method, steam distillation method, ultrasonic extraction method, elution method, and compression method can be extracted. In addition, the desired extract may be further subjected to a conventional fractionation process, may be purified using a conventional purification method, may be diluted, concentrated or dried, and the practice is not limited.

이하, 본 발명을 하기의 실시예를 통해 보다 상세히 설명한다. 그러나 이들 예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through the following examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.

(실시예 1)(Example 1)

아구아헤로부터 오일을 추출하였다. Oil was extracted from Aguahe.

구체적으로 아구아헤를 깨끗이 세척한 후, 절단하고, 건조하였다. 그리고 착유기를 이용하여 아구아헤 오일을 수득하였다. Specifically, the aguahe was thoroughly washed, cut, and dried. And aguahe oil was obtained by using an expeller.

(실시예 2)(Example 2)

실시예 1의 아구아헤 오일에 후피향나무 추출물 및 계수나무껍질 추출물을 1:0.5:0.5중량비로 혼합하였다.The Aguahe oil of Example 1 was mixed with the extracts of fenugreek and cassia bark extract in a weight ratio of 1:0.5:0.5.

이때, 상기 후피향나무 추출물은 후피향나무의 건잎 100g에 혼합용매(에탄올 60중량%, 물 40중량%) 1kg을 가하고, 20℃에서 48시간 교반하여 추출물을 추출하였다. 그리고 이를 250mesh 여과포와 0.5㎛ 필터페이퍼로 여과한 후, 회전감압농축기를 이용하여 50℃에서 감압농축하고, 동결건조하였다. 제조된 추출물을 60Co 감마선(10 kGy/H, 25 kGy)으로 조사 멸균하여 사용하였다. At this time, as for the extract of the H. japonica, 1 kg of a mixed solvent (60% by weight of ethanol, 40% by weight of water) was added to 100 g of dried leaves of the H. japonica, and the extract was extracted by stirring at 20° C. for 48 hours. Then, it was filtered with a 250 mesh filter cloth and 0.5 μm filter paper, and concentrated under reduced pressure at 50° C. using a rotary vacuum concentrator and freeze-dried. The prepared extract was used after sterilization by irradiation with 60Co gamma rays (10 kGy/H, 25 kGy).

그리고 상기 계수나무껍질 추출물은 계수나무의 껍질 100g에 혼합용매(에탄올 60중량%, 물 40중량%) 1kg을 가하고, 20℃에서 48시간 교반하여 추출물을 추출하였다. 그리고 이를 250mesh 여과포와 0.5㎛ 필터페이퍼로 여과한 후, 회전감압농축기를 이용하여 50℃에서 감압농축하고, 동결건조하였다. 제조된 추출물을 60Co 감마선(10 kGy/H, 25 kGy)으로 조사 멸균하여 사용하였다. And the cassia bark extract was extracted by adding 1 kg of a mixed solvent (60% by weight of ethanol, 40% by weight of water) to 100 g of bark of cassia, and stirring at 20° C. for 48 hours. Then, it was filtered with a 250 mesh filter cloth and 0.5 μm filter paper, and concentrated under reduced pressure at 50° C. using a rotary vacuum concentrator and freeze-dried. The prepared extract was used after sterilization by irradiation with 60Co gamma rays (10 kGy/H, 25 kGy).

(시험예 1)(Test Example 1)

본 발명에 따른 화장료 조성물의 보습력을 확인하기 위하여 다음과 같은 실험을 실시하였다. In order to confirm the moisturizing power of the cosmetic composition according to the present invention, the following experiment was conducted.

구체적으로는 Cutometer MPA 580 기기를 이용하여 임의로 정한 실험자의 피부 수분 함량을 측정하고, 시료를 파이펫(pipet)을 이용하여 정확히 10㎕로 취한 뒤, 이를 임의로 선정한 실험자의 피부에 떨어뜨리고 면봉으로 가볍게 발라주었다. Specifically, the skin moisture content of a randomly selected experimenter is measured using the Cutometer MPA 580 instrument, and the sample is taken in exactly 10 μl using a pipet, dropped on the randomly selected experimenter's skin, and lightly with a cotton swab. it was painted

그리고 상기한 조성물을 도포한 120분 후 상기 조성물이 도포된 부위의 수분 함량 변화를 측정하고, 그 결과를 표 1에 나타내었다. 단, 상기 보습 테스트는 온도 22~23℃, 습도 17~18%의 조건에서 수행되었고, 양성대조군으로는 보습제로 흔히 사용되는 화학 성분인 1,3-부틸렌 글리콜을 사용하였다. 또한, 상기 시료로는 실시예 1 또는 2의 조성물에 1,3-부틸렌 글리콜을 1:9 중량비로 혼합하여 사용하였다.And after 120 minutes of application of the composition, a change in the moisture content of the portion to which the composition was applied was measured, and the results are shown in Table 1. However, the moisturizing test was performed under conditions of a temperature of 22 to 23° C. and a humidity of 17 to 18%, and as a positive control, 1,3-butylene glycol, a chemical component commonly used as a moisturizer, was used. In addition, as the sample, 1,3-butylene glycol was mixed with the composition of Examples 1 or 2 in a weight ratio of 1:9 and used.

시험예 1 결과Test Example 1 Results 구분division 양성대조군positive control 실시예 1Example 1 실시예 2Example 2 수분함량(%)Moisture content (%) 27.127.1 35.535.5 37.137.1

상기 표 1에서 확인할 수 있는 바와 같이, 본 발명에 따른 실시예 1, 2를 처리한 결과, 양성대조군인 1,3-부틸렌 글리콜과 대비하였을 때 높은 수분 함량이 유지되는 것을 확인할 수 있었다.As can be seen in Table 1, as a result of the treatment of Examples 1 and 2 according to the present invention, it was confirmed that a high water content was maintained when compared with 1,3-butylene glycol as a positive control.

(시험예 2)(Test Example 2)

본 발명에 따른 화장료 조성물의 항염증 효능을 확인하기 위하여 다음과 같은 실험을 실시하였다. In order to confirm the anti-inflammatory effect of the cosmetic composition according to the present invention, the following experiment was conducted.

구체적으로는, DMEM(in 10% FBS, 1% Penicillin/ streptomysin)을 배지로 사용하여, Raw264.7 세포를 96 well plate에 각 well 당 5.0×104 이 되도록 접종(seeding)하였다. 접종 후 24시간이 경과하면 염증을 활성화(activation)시키기 위해 200ng/ml의 LPS를 넣은 배지(media)로 바꿔주고, 시료를 농도별로 배지에 처리하였다. 그리고 24시간 후, Griess reagent system을 이용하여 NO(Nitric oxide) 생성량을 ELISA를 이용하여 550nm에서 흡광도(Optical Density)를 측정하였다. 그리고 그 결과를 도 1에 나타내었다.Specifically, using DMEM (in 10% FBS, 1% Penicillin/streptomysin) as a medium, Raw264.7 cells were seeded in a 96-well plate to 5.0×10 4 per well. When 24 hours have elapsed after inoculation, the medium containing 200 ng/ml of LPS was changed to activate inflammation, and the samples were treated in the medium by concentration. And 24 hours later, using the Griess reagent system, the amount of NO (nitric oxide) production was measured by ELISA at 550 nm for absorbance (Optical Density). And the result is shown in FIG.

도 1에서 확인할 수 있는 바와 같이, 본 발명에 따른 실시예 1, 2를 처리한 결과, 우수한 NO(Nitric oxide) 생성 억제 활성을 확인할 수 있었다. As can be seen in FIG. 1 , as a result of the treatment of Examples 1 and 2 according to the present invention, excellent NO (nitric oxide) production inhibitory activity was confirmed.

(시험예 3)(Test Example 3)

본 발명에 따른 화장료 조성물의 미백효과를 검정하기 위해 티로시나제(tyrosinase)라는 효소의 기능이 억제되는 정도를 평가하였다. 티로시나제는 생체 내에서 티로신(tyrosine)이라는 물질의 산화과정을 촉진하여 멜라닌이 생성되게 도와주는 효소이다. 멜라닌이란 흑색의 고분자로서 생체 내에서 상기와 같은 산화에 의해 멜라닌이 형성되어 피부 상층부로 이동하게 되면 피부가 검게 변하고 기미, 주근깨를 생성하게 된다. 시험방법은 아래와 같다.In order to test the whitening effect of the cosmetic composition according to the present invention, the degree of inhibition of the function of an enzyme called tyrosinase was evaluated. Tyrosinase is an enzyme that helps the production of melanin by accelerating the oxidation process of a substance called tyrosine in a living body. Melanin is a black polymer, and when melanin is formed by oxidation in the living body and moves to the upper layer of the skin, the skin turns black and produces blemishes and freckles. The test method is as follows.

시료 0.9㎖, 0.1M 인산완충액(pH 6.8) 1.0㎖ 및 1.5㎜ L-티로신 용액 1.0㎖을 넣은 후, 37℃에서 10분간 유지시켰다. 머쉬룸 티로시나제(1,500 units/㎖) 0.1㎖를 첨가하여 37℃에서 10분간 반응시킨 후, UV-vis spectrophotometer(Smartspec Plus, Biorad, USA)를 사용하여 475㎚에서 흡광도를 측정하여 티로시나제에 대한 저해율을 측정하였다.0.9 ml of sample, 1.0 ml of 0.1M phosphate buffer (pH 6.8), and 1.0 ml of 1.5 mm L-tyrosine solution were added, and then maintained at 37° C. for 10 minutes. After adding 0.1 ml of mushroom tyrosinase (1,500 units/ml) and reacting at 37° C. for 10 minutes, absorbance was measured at 475 nm using a UV-vis spectrophotometer (Smartspec Plus, Biorad, USA) to measure the inhibition rate for tyrosinase. did.

상기 티로시나제 활성저해시험을 위한 대조군은 시료 대신 완충용액을 넣어 같은 방법으로 측정하였으며, 티로시나제 대신 완충용액을 넣어 시료와 대조군에 대한 각각의 색 보정값을 얻은 경우로 설정하였다. 효과를 판단하기 위해 실험에 사용한 양성 대조군으로는 여러 연구결과에서 미백효과가 뛰어나다고 알려진 알부틴(Arbutinsynthetic, Sigma)을 사용하였다. 그리고 하기 수학식 1을 이용하여 티로시나제 저해율을 수치로 계산하여 그 결과를 하기 표 2에 나타내었다. 하기 표 2에서 IC50은 티로시나제 활성을 50% 저해하는데 소요되는 시료의 농도이며, 값이 작을수록 저해율이 높음을 나타낸다.The control for the tyrosinase activity inhibition test was measured in the same manner by adding a buffer solution instead of the sample, and a case where each color correction value for the sample and the control was obtained by adding a buffer solution instead of tyrosinase was set. As a positive control used in the experiment to determine the effect, arbutinsynthetic (Sigma), which is known to have excellent whitening effect in various studies, was used. And the tyrosinase inhibition rate was calculated numerically using Equation 1 below, and the results are shown in Table 2 below. In Table 2 below, IC 50 is the concentration of the sample required to inhibit tyrosinase activity by 50%, and the smaller the value, the higher the inhibition rate.

(수학식 1)(Equation 1)

티로시나제 저해율(%) = 100 - {(시료의 흡광도 / 대조군의 흡광도)×100}Tyrosinase inhibition rate (%) = 100 - {(absorbance of sample / absorbance of control) × 100}

시험예 3 결과Test Example 3 Results 구분division 티로시나제 저해율(IC50, ug/㎖)Tyrosinase inhibition rate (IC 50 , ug/ml) 실시예 1Example 1 40.240.2 실시예 2Example 2 29.829.8 알부틴arbutin 30.530.5

상기 표 2에서 확인할 수 있는 바와 같이, 본 발명에 따른 실시예 2는 강력한 미백제로 알려진 알부틴과 거의 유사한 수준의 미백 효과를 나타냄을 확인할 수 있었다.As can be seen in Table 2, it was confirmed that Example 2 according to the present invention exhibited a whitening effect at a level almost similar to that of arbutin, which is known as a powerful whitening agent.

(시험예 4)(Test Example 4)

본 발명에 따른 화장료 조성물의 피부주름 억제 및 개선 효과를 검정하기 위하여 엘라스테이즈 활성저해시험(elastase inhibition activity test)을 실시하였다. 엘라스틴은 피부 진피 내 매트릭스층을 구성하는 성분으로 피부가 노화됨에 따라 엘라스틴이 분해되고 진피 내 매트릭스층이 무너지며 주름이 생기게 된다. 이러한 원리를 이용하여 엘라스틴(elastin)을 분해하는 효소인 엘라스테이즈의 활성을 측정하는 방법으로 엘라스테이즈 기질인 N-석시닐-(Ala)3 p-니트로아닐린(N-succinyl-(Ala)3 p-nitroaniline, Sigma)을 이용하여 p-니트로아닐린이 분해되면서 생기는 색의 변화를 405㎚의 파장에서 흡광도를 측정함으로써, 엘라스테이즈 활성을 측정하는 방법이다. 완충액은 pH 8.0, 0.267 M Trizma-HCl (Sigma), 기질액은 8.8㎜ N-석시닐-(Ala)3 p-니트로아닐린, 효소액은 돼지췌장 엘라스테이즈를 10ug/㎖ (Elastase, Sigma, USA)의 농도로 사용하였다. 완충액 60㎕, 기질액 20㎕와 시료를 농도별로 3차 증류수에 희석한 시료액 100㎕를 섞은 후, 효소액 20㎕를 넣어 25℃ 항온수조에서 15분간 반응시켜 p-니트로아닐린의 생성량을 마이크로플레이트 판독기(UVT-06685, Thermo max, USA)를 사용하여 파장 405㎚에서 측정하였다. 이때, 엘라스테이즈 활성도를 측정하기 위한 대조군은 시료 대신 3차 증류수를 넣어 같은 방법으로 측정하였으며, 효소액 대신 3차 증류수를 넣어 각각에 대한 색보정값을 얻은 경우를 설정하였다. 시험에 사용한 시료의 엘라스테이즈 활성저해효과를 판단하기 위해 엘라스테이즈의 특이 저해제인 PMSF(phenylmethyls㎕fonyl fluoride;Sigma, USA)를 대조군으로 설정하였고, 하기 수학식 2를 이용하여 엘라스테이즈 저해율을 수치로 계산하여 그 결과를 표 3에 나타내었다. 하기 표 3에서 IC50은 엘라스테이즈 활성을 50% 저해하기 위해 소요되는 시료의 농도이며, 값이 작을수록 저해율이 높음을 나타낸다.In order to test the skin wrinkle suppression and improvement effect of the cosmetic composition according to the present invention, an elastase inhibition activity test was performed. Elastin is a component of the matrix layer in the dermis of the skin, and as the skin ages, elastin is decomposed and the matrix layer in the dermis collapses and wrinkles occur. This is a method for measuring the activity of elastase, an enzyme that degrades elastin, using this principle. N-succinyl-(Ala)3 p-nitroaniline (N-succinyl-(Ala) 3 p-nitroaniline, Sigma) is used to measure the color change caused by the decomposition of p-nitroaniline by measuring the absorbance at a wavelength of 405 nm to measure the elastase activity. The buffer solution was pH 8.0, 0.267 M Trizma-HCl (Sigma), the substrate solution was 8.8 mm N-succinyl-(Ala)3 p-nitroaniline, and the enzyme solution was 10ug/ml of porcine pancreatic elastase (Elastase, Sigma, USA). ) was used. After mixing 60 μl of buffer, 20 μl of substrate solution, and 100 μl of sample solution diluted in tertiary distilled water for each concentration, 20 μl of enzyme solution was added and reacted in a constant temperature water bath at 25° C. for 15 minutes to measure the amount of p-nitroaniline produced by microplate. Measurements were made at a wavelength of 405 nm using a reader (UVT-06685, Thermo max, USA). In this case, the control group for measuring the elastase activity was measured in the same way by adding tertiary distilled water instead of the sample, and the case where tertiary distilled water was added instead of the enzyme solution to obtain color correction values for each was set. In order to determine the elastase activity inhibitory effect of the sample used for the test, PMSF (phenylmethylsulfonyl fluoride; Sigma, USA), which is a specific inhibitor of elastase, was set as a control, and elastase inhibition rate using Equation 2 below was calculated numerically and the results are shown in Table 3. In Table 3 below, IC 50 is the concentration of the sample required to inhibit the elastase activity by 50%, and the smaller the value, the higher the inhibition rate.

(수학식 2)(Equation 2)

엘라스테이즈 활성저해율(%) = 100 - {(시료의 흡광도/대조군의 흡광도)×100}Elastase activity inhibition rate (%) = 100 - {(absorbance of sample / absorbance of control group) × 100}

실험예 4 결과Experimental Example 4 Results 구분division 엘라스테이즈 활성저해율(IC50, mg/㎖)Elastase activity inhibition rate (IC50, mg/ml) 실시예 1Example 1 3.543.54 실시예 2Example 2 2.122.12 PMSFPMSF 0.070.07

상기 표 3에서 확인할 수 있는 바와 같이, 본 발명에 따른 실시예 2는 우수한 주름개선 효과를 나타냄을 확인할 수 있었다.As can be seen in Table 3, it was confirmed that Example 2 according to the present invention exhibited an excellent anti-wrinkle effect.

이하 상기한 시험예들의 결과를 근거로 하여 본 발명의 아구아헤 오일을 포함하는 화장료를 조성하여 제시한다. 그러나 본 발명의 조성물을 하기의 제조예들로 한정하고자 하는 것은 아니다. 이때, 하기의 제조예들에 사용된 아구아헤 오일, 후피향나무 추출물, 계수나무껍질 추출물은 상기 실시예 1, 2를 통해 제조한 것을 사용하였다.Hereinafter, based on the results of the above test examples, a cosmetic composition containing aguahe oil of the present invention is formulated and presented. However, it is not intended to limit the composition of the present invention to the following preparation examples. At this time, the aguahe oil, the quince extract, and the cassia bark extract used in the following preparation examples were those prepared in Examples 1 and 2 above.

(제조예 1 내지 2 및 비교제조예 1)(Preparation Examples 1 to 2 and Comparative Preparation Example 1)

하기 표 4의 배합비로서 통상적인 방법에 의해 수중유형 에멀젼을 제조하였다. 구체적으로는, 정제수에 디소듐이디티에이, 글리세린, 프로판다이올, 1,2-헥산다이올을 용해시키고, 여기에 디스테아레스-100 IPDI, 암모늄아크릴로일디메칠타우레이트/브이피코폴리머를 첨가하여 젤상을 형성하였다. 그리고 실리콘상 및 유상의 성분을 혼합한 후, 상기 젤상에 상기 혼합된 실리콘상과 유상의 성분을 투입하여 호모믹서로 6000rpm의 속도로 교반하면서 10분간 유화하여 수중유형의 화장료 조성물을 제조하였다. 이때, 제조예 2의 제조시에는 상기 정제수에 후피향나무 추출물, 계수나무껍질 추출물을 추가로 용해시켰다.An oil-in-water emulsion was prepared by a conventional method as shown in Table 4 below. Specifically, disodium EDTA, glycerin, propanediol, and 1,2-hexanediol were dissolved in purified water, and disteareth-100 IPDI, ammonium acryloyl dimethyl taurate / VPicopolymer was dissolved therein. was added to form a gel. And after mixing the components of the silicone phase and the oil phase, the components of the silicone phase and the oil phase were added to the gel phase and emulsified for 10 minutes while stirring at a speed of 6000 rpm with a homomixer to prepare an oil-in-water cosmetic composition. At this time, in the preparation of Preparation Example 2, the extract of fenugreek and cassia bark extract was further dissolved in the purified water.

제조예 1 배합비Preparation Example 1 Blending ratio 성분ingredient 제조예 1Preparation Example 1 제조예 2Preparation 2 비교제조예 2Comparative Preparation Example 2 유상paid 아구아헤 오일aguahe oil 66 66 -- 부틸렌글라이콜다이카프릴레이트/다이카프레이트Butylene glycol dicaprylate/dicaprate 2.52.5 2.52.5 2.52.5 다이-C12-13 알킬말레이트Di-C12-13 Alkyl Maleate 77 77 77 하이드로제네이티드폴리(C6-14올레핀)Hydrogenated Poly (C6-14 Olefin) 0.50.5 0.50.5 0.50.5 실리콘상silicone phase 페닐트리메치콘Phenyltrimethicone 22 22 22 사이클로펜타실록세인cyclopentasiloxane 33 33 33 다이메티콘/비닐다이메티콘크로스폴리머Dimethicone/Vinyl Dimethicone Cross Polymer 0.50.5 0.50.5 0.50.5 폴리메틸실세스퀴옥세인polymethylsilsesquioxane 0.70.7 0.70.7 0.70.7 수상Awards 디소듐이디티에이Disodium EDIT 0.30.3 0.30.3 0.30.3 글리세린glycerin 77 77 77 프로판다이올propanediol 33 33 33 1,2-헥산다이올1,2-Hexanediol 0.40.4 0.40.4 0.40.4 디스테아레스-100 IPDIDistealess-100 IPDI 2.52.5 2.52.5 2.52.5 암모늄아크릴로일디메칠타우레이트/브이피코폴리머Ammonium Acryloyl Dimethyl Taurate/Vpicopolymer 0.30.3 0.30.3 0.30.3 후피향나무 추출물Pachyderm extract -- 33 -- 계수나무껍질 추출물Cassia Bark Extract -- 33 -- 정제수Purified water to 100to 100 to 100to 100 to 100to 100

(시험예 5)(Test Example 5)

피부노화가 진행 중인 30대 여성 30명(그룹당 10명)을 대상으로, 상기한 제조예 1, 2 및 비교제조예 1을 12주간 사용토록 한 후, 보습, 탄력, 미백, 주름 개선과 전체적인 만족도를 9점 척도로 평가토록 하였다.(1점은 매우 악화됨, 9점은 매우 개선됨으로 평가) 그리고 그 결과를 하기 표 5에 나타내었다.For 30 women in their 30s (10 per group) who are undergoing skin aging, after using Preparation Examples 1 and 2 and Comparative Preparation Example 1 for 12 weeks, moisturizing, elasticity, whitening, wrinkle improvement and overall satisfaction was evaluated on a 9-point scale (1 point was evaluated as very bad, 9 points as very improved).

실험예 5 결과Experimental Example 5 Results 구분division 보습Moisturizing 탄력elasticity 미백Whitening 주름wrinkle 만족도satisfaction 제조예 1Preparation Example 1 7.87.8 7.57.5 5.15.1 5.15.1 6.86.8 제조예 2Preparation 2 7.97.9 7.87.8 7.87.8 7.27.2 7.87.8 비교제조예 1Comparative Preparation Example 1 5.15.1 4.64.6 4.14.1 4.04.0 4.54.5

상기 표 5에서 확인할 수 있는 바와 같이, 제조예 1, 2 모두 보습, 탄력, 미백, 주름 개선에 대한 만족도가 높음을 확인하였다. As can be seen in Table 5, it was confirmed that both Preparation Examples 1 and 2 had high satisfaction with moisturizing, elasticity, whitening, and wrinkle improvement.

(시험예 6)(Test Example 6)

피부노화가 진행 중인 40대 이상 여성 10명을 대상으로, 상기한 제조예 2를 12주간 눈가주름 부위에 사용하게 한 후, 눈가 주름 주형(replica)을 영상분석법으로 피부미세주름측정기(SV-600, C+K,Germany)를 이용하여 주름의 깊이(㎛)를 측정하였다. For 10 women in their 40s or older who are undergoing skin aging, the above-mentioned Preparation Example 2 was used on the periorbital area for 12 weeks. , C + K, Germany) was used to measure the depth of wrinkles (㎛).

그 결과, 12주 후 제조예 2의 주름깊이 감소율은 4.7%이었다.As a result, the wrinkle depth reduction rate of Preparation Example 2 after 12 weeks was 4.7%.

상기 결과에서 확인할 수 있는 바와 같이, 본 발명의 화장료 조성물은 주름개선에 효과가 있음을 확인하였다.As can be seen from the above results, it was confirmed that the cosmetic composition of the present invention is effective in improving wrinkles.

이상, 본 발명을 바람직한 실시예를 사용하여 상세히 설명하였으나, 본 발명의 범위는 특정 실시예에 한정되는 것은 아니며, 첨부된 특허청구범위에 의하여 해석되어야 할 것이다. 또한, 이 기술분야에서 통상의 지식을 습득한 자라면, 본 발명의 범위에서 벗어나지 않으면서도 많은 수정과 변형이 가능함을 이해하여야 할 것이다.As mentioned above, although the present invention has been described in detail using preferred embodiments, the scope of the present invention is not limited to specific embodiments, and should be interpreted by the appended claims. In addition, those skilled in the art will understand that many modifications and variations are possible without departing from the scope of the present invention.

Claims (4)

삭제delete 삭제delete 삭제delete 아구아헤 오일, 후피향나무 추출물 및 계수나무껍질 추출물을 유효성분으로 포함하는 보습 및 항염증용 화장료 조성물로,
상기 화장료 조성물은 수중유형 에멀젼(O/W emulsion)으로, 수상, 유상 및 실리콘상이 혼합된 것이며,
상기 수상은 디소듐이디티에이(Disodium EDTA), 글리세린(Glycerin), 프로판다이올(propanediol), 1,2-헥산다이올(1,2-Hexanediol), 디스테아레스-100 IPDI(disteareth-100 IPDI), 암모늄아크릴로일디메칠타우레이트/브이피코폴리머(Ammonium Acryloyldimethyltaurate/VP Copolymer), 상기 후피향나무 추출물, 상기 계수나무껍질 추출물 및 정제수를 포함하고,
상기 유상은 상기 아구아헤 오일, 부틸렌글라이콜다이카프릴레이트/다이카프레이트(Butylene Glycol Dicaprylate/Dicaprate), 다이-C12-13 알킬말레이트(DI-C12-13 Alkyl Malate) 및 하이드로제네이티드폴리(C6-14올레핀)(Hydrogenated Poly(C6-14 Olefin))를 포함하며,
상기 실리콘상은 페닐트리메치콘(Phenyl Trimethicone), 사이클로펜타실록세인(Cyclopentasiloxane), 다이메티콘/비닐다이메티콘크로스폴리머(Dimethicone/Vinyl Dimethicone Crosspolymer) 및 폴리메틸실세스퀴옥세인(Polymethylsilsesquioxane)을 포함하고,
상기 화장료 조성물 100중량%에 대하여, 상기 아구아헤 오일 0.1~10중량%, 상기 후피향나무 추출물 0.1~5중량% 및 상기 계수나무껍질 추출물 0.1~5중량%만큼 포함하며,
NO(Nitric oxide) 생성 억제 활성, 엘라스타제 저해 활성 및 타이로시나아제 저해 활성을 가지는 것을 특징으로 하는 아구아헤 오일을 포함하는 화장료 조성물.
A cosmetic composition for moisturizing and anti-inflammatory, comprising aguahe oil, fenugreek extract, and cassia bark extract as active ingredients,
The cosmetic composition is an oil-in-water emulsion (O/W emulsion), in which an aqueous phase, an oil phase and a silicone phase are mixed,
The aqueous phase is disodium EDTA, glycerin (Glycerin), propanediol (propanediol), 1,2-hexanediol (1,2-Hexanediol), disteareth-100 IPDI (disteareth-100) IPDI), ammonium acryloyldimethyltaurate / VP Copolymer (Ammonium Acryloyldimethyltaurate / VP Copolymer), the extract of the fenugreek extract, the cassia bark extract and purified water,
The oil phase is the aguahe oil, butylene glycol dicaprylate / dicaprate (Butylene Glycol Dicaprylate / Dicaprate), di-C12-13 alkyl malate (DI-C12-13 Alkyl Malate) and hydrogenated poly (C6-14 Olefin) (Hydrogenated Poly (C6-14 Olefin)),
The silicone phase includes phenyl trimethicone, cyclopentasiloxane, dimethicone/vinyl dimethicone crosspolymer, and polymethylsilsesquioxane, and ,
With respect to 100% by weight of the cosmetic composition, 0.1 to 10% by weight of the aguahe oil, 0.1 to 5% by weight of the fenugreek extract, and 0.1 to 5% by weight of the cassia bark extract,
A cosmetic composition comprising aguahe oil, characterized in that it has nitric oxide (NO) production inhibitory activity, elastase inhibitory activity and tyrosinase inhibitory activity.
KR1020210057182A 2021-05-03 2021-05-03 Cosmetic composition having mauritia flexuosa fruit oil KR102327092B1 (en)

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KR20070019440A (en) * 2005-08-12 2007-02-15 주식회사 케이피이 New compounds isolated from leaves of Ternstroemia japonica and antioxidant using the same
KR20100041243A (en) * 2008-10-13 2010-04-22 주식회사 엘지생활건강 Cosmetic compostion for skin cell regeneration
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KR102195365B1 (en) 2019-06-12 2020-12-30 (주)나우코스 Cosmetic composition for skin moisturizing comprising Mixed extract of rice embryo and fermentation
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KR20070019440A (en) * 2005-08-12 2007-02-15 주식회사 케이피이 New compounds isolated from leaves of Ternstroemia japonica and antioxidant using the same
KR20100041243A (en) * 2008-10-13 2010-04-22 주식회사 엘지생활건강 Cosmetic compostion for skin cell regeneration
KR101198129B1 (en) 2010-04-05 2012-11-12 덕성여자대학교 산학협력단 Anti-inflammatory composition extracted from Artemisisa iwayomogi
KR102195365B1 (en) 2019-06-12 2020-12-30 (주)나우코스 Cosmetic composition for skin moisturizing comprising Mixed extract of rice embryo and fermentation
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