KR102321147B1 - Cosmetic composition comprising Extracts of Ngoc Linh Ginseng, Curcuma longa, and Morinda citrifolia - Google Patents

Abstract

Disclosed is a cosmetic composition for improving whitening comprising an extract of ngokrin ginseng as a first component and an extract of a mixture of steamed turmeric and noni as a second component.

Description

Cosmetic composition comprising Extracts of Ngoc Linh Ginseng, Curcuma longa, and Morinda citrifolia

The present invention relates to a cosmetic composition comprising ngokrin ginseng, turmeric, and noni extract.

Recently, due to the destruction of the ozone layer due to environmental pollution, ultraviolet rays are increasing, and skin color changes, freckles, etc. are increasing. Melanin is reported to be biosynthesized by the action of tyrosinase as a pigment of the skin. Tyrosinase deposits melanin pigment on the skin exposed to UV rays, making the skin color darker or causing spots and freckles. Therefore, in the cosmetics and pharmaceutical industries, research is being actively conducted to develop substances that can prevent and improve skin damage by inhibiting melanin production. So far, research on melanogenesis inhibitors has been mainly focused on the development of tyrosinase inhibitors. Representative tyrosinase inhibitors include chelate-forming substances for copper ions in the tyrosinase active site, reducing agents such as ascorbic acid that reduce quinones to phenols, and and bisulfites that modify tyrosinase itself. For example, as a melanin biosynthesis inhibitor, hydroquinone, ascorbic acid or a derivative thereof, kojic acid, glutathione, a substance having inhibitory activity against tyrosinase such as arbutin is known. . However, hydroquinone has very strong skin irritation, so there is a problem that its use is limited, and ascorbic acid is easily oxidized, causing problems such as discoloration and odor of the formulation. In addition, kojic acid is unstable in solution, so the stability of the formulation is low, and there is a problem of carcinogenicity for the occurrence of thyroid cancer. And in the case of arbutin, its activity is not high, and thiol-based compounds such as glutathione and cysteine have a characteristic unpleasant odor and have a problem in transdermal absorption. there is a problem. Therefore, it is necessary to discover a functional material derived from natural products that can achieve a whitening effect without being harmful to the human body.

Ginseng (Panax ginseng C.A. Meyer) is a plant belonging to the genus Ginseng of the family Orchidaceae and has been used as a herbal medicine in Korea and other Asian countries for a long time. The efficacy of ginseng known so far has been found to have pharmacological effects on the central nervous system, anti-stress, and anti-diabetes derived from saponins.

Turmeric (Curcuma longa) is rich in curcumin, a main ingredient in curry, and is known to have anti-inflammatory and antioxidant effects.

Noni (Morinda citrifolia) has a potato-like fruit with a rough surface and dents. The fruit contains several small brown seeds, and when ripe, it has a foul odor and is not used actively. Recently, research results are known that noni fruit is effective in improving diabetes, gout, vasculitis, and arthritis.

Republic of Korea Publication No. 10-2014-0145393 (2014.12.23)

According to one embodiment, by including an extract of a mixture of Ngoc Linsam (Panax vietnamensis, Ngoc Linh) extract, and a mixture of turmeric (Curcuma longa) and noni (Morinda citrifolia), it effectively inhibits melanin production and has excellent whitening effect than the ethanol extract of conventional ginseng To provide a cosmetic composition that can exhibit

One aspect provides a cosmetic composition for improving whitening comprising an extract of Ngoc Linh (Panax vietnamensis, Ngoc Linh) as a first component, and an extract of a mixture of turmeric (Curcuma longa) and noni (Morinda citrifolia) as a second component .

The ngokrin ginseng may use the whole ginseng, preferably ginseng root may be used. The turmeric may preferably be a rhizome or tuber. The noni may preferably be a fruit.

The present inventors have found that the extract of Ngolin ginseng has the most excellent whitening effect among ginseng, and that the whitening effect is further increased when the extract of the steamed turmeric and noni mixture is further mixed.

In one embodiment, the ngokrinsam extract is heat-treating ngokrinsam; Steaming the heat-treated Ngogrin ginseng; And steamed ngokrin ginseng may be extracted using ethanol as a solvent. The step of heat-treating the ngokrin ginseng may be heat-treating at 120 to 160° C. for 20 to 30 minutes. The step of steaming the heat-treated ngokrin ginseng may be carried out at 60 to 80° C. for 4 to 5 hours.

If Ngocrin ginseng is pretreated with heat treatment and steaming and alcohol extraction is performed, the melanin production inhibitory effect of the extract can be further improved than that of an alcohol extract that is not pretreated.

In one embodiment, the weight ratio of the first component and the second component is 1.1:1 to 2.9:1, 1.3:1 to 2.7:1, 1.5:1 to 2.5:1, 1.8:1 to 2.2:1, or 2 It can be 1:1. When the first component and the second component are mixed in the above weight ratio, the effect of inhibiting melanin production can be further improved.

In one embodiment, the first component and the second component are 0.1 to 10% by weight, 0.2 to 10% by weight, 0.3 to 10% by weight, 0.4 to 10% by weight, 0.5 to 10% by weight, based on the total weight of the cosmetic composition; 1 to 10% by weight, 2 to 10% by weight, or 3 to 10% by weight.

In one embodiment, the second component has a weight ratio of turmeric and noni of 0.8:1 to 1.2:1, 0.9:1 to 1.2:1, 0.8:1 to 1.1:1, 0.9:1 to 1.1:1, or 1 It may be an extraction of a mixture of 1:1.

In one embodiment, the second component may be extracted with 60 to 80% ethanol, or 70% ethanol.

In one embodiment, the turmeric may be turmeric pre-treated by steaming. When ethanol is extracted by steaming turmeric, the effect of inhibiting melanin production can be further improved than the ethanol extract of turmeric that is not steamed.

In one embodiment, the steamed turmeric may be steamed at 40 to 60° C. for 30 minutes to 1 hour, 30 minutes to 50 minutes, or 30 minutes to 40 minutes.

Products to which the cosmetic composition of the present invention can be added include, for example, various skin lotions, skin softeners, skin toners, astringents, lotions, milk lotions, nourishing lotions, massage creams, nourishing creams, hand creams, foundations, and makeup. It may be a base, twin cake, mascara, essence, nourishing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion or body cleanser.

When the cosmetic composition of the present invention is prepared in the form described above, it may contain various bases and additives necessary and appropriate for the formulation of the dosage form. In addition, the type and amount of these components can be easily selected by those skilled in the art.

For example, when the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

As another example, when the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

As another example, when the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, such as suspension agent, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like can be used.

As another example, when the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluoro propellants such as hydrocarbons, propane/butane or dimethyl ether.

As another example, when the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate as carrier components , fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

In addition, the cosmetic composition of the present invention may be used alone or in combination, or may be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the skin condition or taste of the user.

Since it was confirmed that the cosmetic composition according to one embodiment has an excellent effect of inhibiting melanin production, it may have an excellent whitening effect.

1 is a measurement of the cell viability during treatment with different types of ginseng extracts according to an embodiment. MSHg is Korean ginseng extract, MSHn is Korean ginseng extract, MSHj is bamboo jeol ginseng extract, and MSHv is Ngolin ginseng extract.
Figure 2 shows the melanin production inhibitory effect of different types of ginseng ethanol extract according to an embodiment.
Figure 3 shows the melanin production inhibitory effect of the ethanol extract of ngokrinsam at different temperatures according to an embodiment.
Figure 4 shows the melanin production inhibitory effect of the ethanol extract of ngokrinsam steamed at different temperatures according to an embodiment.
Figure 5 shows the melanin production inhibitory effect of the ethanol extract of ngokrinsam, which was heat-treated and steamed at different temperatures according to an embodiment.
Figure 6 shows the melanin production inhibitory effect of the ethanol extract of ngokrinsam that is not heat-treated and steamed at different temperatures according to one embodiment.
7 shows the melanin production inhibitory effect of the ethanol extract of turmeric, the ethanol extract of noni, and the ethanol extract of turmeric and noni.
8 is a comparison of the melanin production inhibitory effect of the ethanol extract of steamed turmeric and the ethanol extract of steamed noni.

Hereinafter, one or more specific embodiments will be described in more detail through examples. However, these examples are for illustrative purposes of one or more embodiments, and the scope of the present invention is not limited to these examples.

Example 1: Confirmation of cell viability and whitening effect of ginseng extract

1-1. Preparation of 4 kinds of ginseng extract

The whitening effects of Korean ginseng (Panax ginseng), Korean ginseng (P. notoginseng), bamboo japonicas (P. japonicas), and Ngoc Linh (P. vietnamensis, Ngoc Linh) were compared. Ngoc Linh Ginseng was obtained from Ngoc Linh Mountain in Kon tum Province. The root of each ginseng was used for 2-3 years, and stored at -70°C until use.

Each root of ginseng was thawed, and 500 g was immersed in 5 L of 70% ethanol solvent, extracted at room temperature for 24 hours, and filtered through filter paper. The amount of alcohol used was about 10 times the weight of steamed ginseng.

1-2. B16F10 melanoma cell line culture

B16F10 mouse melanoma cells were distributed from the Korean cell line bank, and 10% FBS (fetal bovine serum, Welgene) and 1% penicillin-streptomycin (Gibco BRL) were used in DMEM medium supplemented with 37°C, 5 % CO ₂ Incubated under conditions. After subculture twice a week, the ^{cells were seeded at about 1 X 10 4} cells/well in a 24-well plate and cultured for 24 hours. An appropriate number of cells was maintained using trypsin to prevent cell overdensity.

1-3. Measurement of viability of B16F10 melanoma cells

^{B16F10 cells were aliquoted at 3 X 10 4} cells/well in a 6-well plate for cell culture, and 200 μg/ml of the steamed ginseng extract was treated per well. After 3 days, 200 μl of diluted 0.5 mg/ml tetrazolium bromide salt (MTT, Amresco, Solon, USA) was dispensed and _{incubated in a CO 2} incubator for 2 hours, then the medium and MTT reagent were removed and DMSO was added 2 ml each. After dissolving the formazin generated in the wells, 200 μl of each was dispensed into a 96 well plate, and absorbance was measured at 540 nm with an ELISA reader. Toxicity to each cell was expressed as a percentage by obtaining the average absorbance of each control group.

According to FIG. 1, all groups treated with each ginseng extract had excellent cell viability, so side effects were not expected to be significant when used as a cosmetic composition.

1-4. Measurement of melanin production in B16F10 melanoma cells

B16F10 cells were treated with α-melanocyte stimulating hormone (α-MSH) and 4 types of ginseng extracts prepared in Example 1-1 at 100 μg/ml, cultured for 2 days, and cells were collected and washed with PBS. After that, 1N NaOH containing 10% DMSO was added and incubated at 80° C. for 1 hour to dissolve melanin, and then absorbance was measured at 425 mm with an ELISA reader (Molecular Device, Sunnyvale, CA, USA).

Referring to FIG. 2 , melanin production was increased in the group treated with α-MSH, but the increase in melanin content was inhibited in the group treated with Korean ginseng, Korean ginseng, jeonchilsam, bamboo jeolsam, and Ngolin ginseng extract. It was confirmed that the melanin production inhibitory effect was the most excellent.

Example 2: Confirmation of whitening effect according to the method of extracting ngokrin ginseng

2-1. Confirmation of effect change according to extraction temperature

To establish the optimal extraction temperature, ngokrin ginseng was extracted with 70% ethanol at room temperature for 24 hours, extracted at 40°C for 8 hours, extracted at 50°C for 4 hours, and extracted at 60°C for 2 hours. Each extract was tested in the same manner as in Examples 1-4 to measure melanin production.

Referring to FIG. 3 , it was confirmed that the Ngolin ginseng extract extracted at 50° C. for 4 hours had the best melanin production inhibitory effect.

2-2. Confirmation of effect change of steamed ngokrin ginseng

The melanin production inhibitory effect of the 70% alcohol extract of the steamed Ngolin ginseng was confirmed.

Steamed ginseng was prepared by putting ginseng in a steamer and steaming it at 50 °C, 60 °C, 70 °C, 80 °C, 90 °C, and 100 °C for about 4 hours at different temperatures.

Ginseng steamed at each temperature was extracted with 70% alcohol at a temperature of 50° C. for 4 hours, and the amount of melanin production was measured in the same manner as in Example 1-4.

Referring to Figure 4, it was confirmed that the melanin production inhibitory effect of the extract extracted from ginseng steamed for 4 hours at 70 ℃ is the most excellent.

2-3. Confirmation of effect change of steamed ginseng after heat treatment

Ngolin ginseng heat-treated at different temperatures was prepared and each was steamed and extracted with ethanol to compare the effect of inhibiting melanin production.

Heat-treated ngokrin ginseng was prepared by heat-treating in an oven at 100 °C, 120 °C, 140 °C, and 160 °C for 25 minutes, respectively.

Steaming and ethanol extraction were performed in the same manner as in the above example, and steaming was performed at 70° C. for 4 hours, and ethanol extraction was performed at 50° C. for 4 hours.

Referring to FIG. 5 , it was confirmed that the melanin production inhibitory effect of the ethanol-extracted extract after steaming ngokrin ginseng heat-treated at 140° C. was the most excellent, and the effect decreased when the temperature was increased.

In addition, compared with the results of Figure 3, the ethanol extract of Ngolin ginseng, which had undergone the heat treatment and steaming steps, significantly increased the melanin production inhibitory effect when compared to the ethanol-extracted extract immediately after thawing without going through these steps.

2-4. Confirmation of effect change of ethanol-extracted ginseng after heat treatment

The change in the effect of ethanol-extracted ginseng after drying and omitting the steaming step in Example 2-3 was confirmed.

Drying conditions and ethanol extraction conditions of ngokrin ginseng were carried out in the same manner as in Example 2-3.

Referring to Figure 6, when ethanol extraction without steaming after drying, the melanin production inhibitory effect is inferior to that of the Ngoulin ginseng extract treated with both drying and steaming, and it can be confirmed that there is no significant difference from the case of extracting as it is after thawing. there was.

Therefore, it was thought to promote the extraction of melanin production inhibitory components during the ethanol extract of Ngogrin ginseng by drying and steaming.

Example 3: Confirmation of whitening effect of turmeric and noni

3-1. Confirmation of melanin production inhibitory effect of ethanol extracts of turmeric and noni

The ethanol extract of turmeric, the ethanol extract of noni, and the ethanol extract of turmeric and noni were extracted by the methods of Examples 2-1 and 2-2 to confirm the effect of inhibiting melanin production. Specifically, 500 g of turmeric was used for the ethanol extract of turmeric, 500 g of noni was used for the ethanol extract of noni, and 500 g of turmeric and noni were used in a 1:1 weight ratio, and 70% ethanol was immersed in 5L. The temperature and time were extracted at room temperature for 24 hours, extracted at 40°C for 8 hours, extracted at 50°C for 4 hours, and extracted at 60°C for 2 hours.

Referring to Figure 7, turmeric extract, noni extract, and turmeric and noni extract were judged to have the effect of inhibiting melanin production compared to the control group, but the effect was not large, and the difference according to the temperature conditions during extraction was also confirmed that there is no significant difference. became

3-2. Confirmation of whitening effect of steamed turmeric and steamed noni mixed extract

The whitening effect was confirmed by steaming turmeric and noni at 70° C. for 4 hours and extracting them at room temperature with 70% ethanol. The temperature and time were extracted at room temperature for 24 hours, extracted at 40°C for 8 hours, extracted at 50°C for 4 hours, and extracted at 60°C for 2 hours.

Referring to Figure 8, when the steamed turmeric was extracted with ethanol, the melanin production inhibitory effect was further improved, but it was confirmed that the steamed noni rather decreased the melanin production inhibitory effect. In the case of noni, it is thought that this is because the tissue is destroyed during the steaming process, resulting in loss of components. In addition, it was confirmed that there was no significant difference in the difference according to the temperature conditions during extraction.

Example 4: Confirmation of whitening effect of a mixture of Ngolin ginseng extract and steamed turmeric and noni extract

The ethanol extract of ngokrinsam (HSEG) prepared by the method of Example 2-4, the steamed turmeric ethanol extract (SEC) of Example 3-2, and the noni ethanol extract (EN) of Example 3-1 were varied. The melanin production inhibitory effect was confirmed by mixing.

According to Table 1, as a result of mixing HSEG and SEC in a weight ratio of 1:1 and confirming the melanogenesis inhibitory effect, it was found that the melanogenesis inhibitory effect was lower than the median value (expected value) of HSEG and SEC.

As a result of mixing HSEG and EN in a weight ratio of 1:1 and confirming the melanogenesis inhibitory effect, it was confirmed that the melanogenesis effect was similarly lower than the median value (expected value) of HSEG and EN.

As a result of mixing HSEG, SEC, and EN in different ratios, it was confirmed that the melanogenesis inhibitory effect was significantly improved compared to the case where only the HSEG extract was treated when the weight ratio of HSEG:SEC:EN was 2:1:1.

Example 5: Confirm the whitening effect of the extract extracted by mixing heat-treated and steamed Ngolin ginseng, steamed turmeric and noni

Ngocrin ginseng, which has been subjected to heat treatment and steaming, turmeric, which has undergone a steaming step, and untreated noni were mixed differently, immersed in 70% ethanol, and extracted at 50° C. for 4 hours. The pretreatment process of ngokrin ginseng and turmeric is the same as described in the above embodiment.

Referring to Table 2, it is better to extract and mix steamed turmeric and noni together and separately extract and mix heat-treated and steamed ngocrinsam, steamed turmeric, and noni rather than extracting and mixing ethanol respectively. It was confirmed that the melanin production inhibitory effect was further improved.

Claims (9)

Ngocrin ginseng extract as a first component,
An extract of a mixture of steamed turmeric and noni as a second component,
A cosmetic composition for improving whitening, comprising:
The ngokrin ginseng extract,
Heat-treating ngokrin ginseng;
Steaming the heat-treated Ngogrin ginseng; and
Which is prepared in the step of ethanol extraction of steamed ngokrin ginseng,
cosmetic composition. delete The method of claim 1,
The heat treatment of the ngokrin ginseng is to heat treatment at 120 to 160 ° C for 20 to 30 minutes,
The step of steaming the heat-treated ngokrin ginseng is to steam for 4 to 5 hours at 60 to 80 ℃,
cosmetic composition. The method of claim 1,
The step of extracting the ethanol will be extracted at a temperature of 40 to 60 ℃,
cosmetic composition. The method of claim 1,
The weight ratio of the first component and the second component is 1.1:1 to 2.9:1,
cosmetic composition. The method of claim 1,
The second component is a mixture of turmeric and noni in a weight ratio of 0.8:1 to 1.2:1 is extracted,
cosmetic composition. The method of claim 1,
The second component is extracted with 60 to 80% ethanol,
cosmetic composition. delete 8. The method of claim 7,
The steamed turmeric is steamed at 40 to 60° C. for 30 minutes to 1 hour,
cosmetic composition.

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