KR102314188B1 - Functional snack composition for pets - Google Patents

Abstract

The present invention relates to a functional snack composition for companion animals comprising meat, fish, seafood and a natural mixed extract, and a method for preparing the snack composition. The functional snack composition for companion animals of the present invention exhibits high antibacterial and anti-inflammatory activity against oral harmful bacteria by including natural components. Therefore, the functional snack composition for companion animals of the present invention can help to prevent stomatitis in companion animals, has excellent palatability, and thus can be used as a healthy snack for the companion animals.

Description

Functional snack composition for pets {Functional snack composition for pets}

The present invention relates to a functional snack composition for companion animals comprising meat, fish, seafood and a natural mixed extract, and a method for preparing the snack composition.

Recently, the domestic and overseas companion animal-related business market is gradually expanding. This is analyzed to be the cause of the increase in single-person households and the strengthening of the family concept for companion animals. According to this change in culture, businesses related to raising companion animals are showing rapid growth, and the companion animal product market is also growing rapidly through the launch of premium products and diversification of items. In fact, the average expenditure on pet-related items per household by Statistics Korea has increased by 9.6% over the past five years. Among them, the pet food sector shows the largest increase. As the tendency to think of companion animals as family increases, interest in and investment in food for companion animals also increases, leading to more high-quality products. According to the Korea Pet Food Association, the size of the pet food market grew from KRW 732.3 billion in 2014 to KRW 969.6 billion in 2016, and is currently forecast to reach KRW 1 trillion. The recent trend in purchasing food for companion animals is the increase in consumption of high-quality feed, the increase in purchase intention to purchase prescription food that can treat diseases as well as high nutritional value, and the demand for wet food with high protein and moisture content as the population of cats increases. It can be counted as an increase.

Food for companion animals can be divided into stocks and snacks, and stocks belong to feed, and there are various types according to the age and characteristics of companion animals. Snacks refer to food for companion animals, excluding feed food, and are divided into various products and types according to the type of raw material and processing characteristics. As of 2016, the companion animal snack market was estimated at KRW 82.2 billion for dogs and KRW 17.2 billion for cats, with a total size of KRW 99.4 billion, accounting for about 18% of the total companion animal food market (Euromonitor (2017)) Pet food in South Korea; Dog, Cat, Others).

Under this background, the present inventors have made intensive efforts to develop a functional snack composition for companion animals that can improve functionality and quality while maintaining the preference of companion animals, and as a result, by including a natural extract, it helps to prevent stomatitis of companion animals By confirming that it can give, the present invention was completed.

An object of the present invention is one or more meat selected from the group consisting of chicken, beef, and duck; at least one fish selected from the group consisting of salmon, red snapper, and tuna; at least one seafood selected from the group consisting of crab meat, squid, scallops, and shrimp; And it is to provide a snack composition for companion animals, including a mixed extract of wild pepper, gold, and windbreak.

Another object of the present invention is to provide a method for preparing the snack composition.

This will be described in detail as follows. Meanwhile, each description and embodiment disclosed in the present invention may be applied to each other description and embodiment. That is, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be considered that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention for achieving the above object is one or more meat selected from the group consisting of chicken, beef, and duck; at least one fish selected from the group consisting of salmon, red snapper, and tuna; at least one seafood selected from the group consisting of crab meat, squid, scallops, and shrimp; And it provides a snack composition for companion animals, which includes a mixed extract of sancho, gold, and windbreak.

As used herein, the term "companion animal" refers to dogs, cats, rabbits, ferrets, guinea pigs, and hamsters raised for the purpose of companionship, and the scope of the animals is not limited as long as they are animals that live together with humans. In the present invention, the companion animal may specifically be a dog or a cat, and more specifically may be a cat, but is not limited thereto.

As used herein, the term "snack for pets" refers to food for companion animals, excluding feed food. Snacks for companion animals can be divided into types such as sashimi/jerky, jerky, chewing gum, wet snack/sausage, can/pouch, biscuit/cookie, and dry food. The form of the snack in the present invention is not limited, but may preferably be in the form of a wet snack or a can/pouch.

The snack composition for companion animals of the present invention comprises at least one meat selected from the group consisting of chicken, beef, and duck; at least one fish selected from the group consisting of salmon, red snapper, and tuna; and at least one seafood selected from the group consisting of crab meat, squid, scallops, and shrimp. Each of the above raw materials may be processed or treated in a manner conventionally performed in the art and included in the snack composition of the present invention, preferably in the form of ground meat, but is not limited thereto.

The snack composition for companion animals of the present invention includes a mixed extract of sancho, gold, and windbreak.

In the present invention, the term "extract" is an active ingredient contained in the extraction raw material by contacting the solvent and the extraction raw material under specific conditions, so long as the ingredients contained in the raw material can be separated, regardless of the extraction method or the type of the active ingredient, all of them will be included. can For example, extracts of components soluble in solvents from raw materials using water or organic solvents, and extracts obtained by extracting only specific components such as specific components of raw materials, such as oil, may be included. The extract may be powdered after drying, or may be obtained by various conventionally known extraction methods such as hot water extraction and ethanol extraction. The extract is selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, acetone, petroleum ether, ethyl acetate, butyl acetate, trichloromethane, dichloromethane, chloroform, hexane and 1,3-butylene glycol It can be extracted with one or more solvents. Preferably, it may be extracted for 5 hours in hot water of 80 to 90 ℃ by adding distilled water 10 times the weight of the natural product powder, but is not limited thereto.

The extract may also include an extract that has undergone a conventional purification process. For example, the extract is separated using an ultrafiltration membrane having a constant molecular weight cut-off value, and various purification methods are additionally performed such as separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity). It may include a fraction obtained through

The "mixed extract" of the present invention may be extracted by mixing and extracting the powder of sancho, gold, and windbreak, and may be obtained by extracting and mixing the raw materials of natural products, but is not limited thereto. In the mixed extract of the present invention, Sancho, gold, and windbreak powder were mixed in a weight ratio of 1:1:1, but if Sancho, gold, and windbreak were mixed to exhibit synergistic activity compared to each single extract, the content ratio is not limited

In a specific embodiment of the present invention, the mixed extract of sancho, gold, and windbreak has synergistic activity in terms of antibacterial activity against oral harmful bacteria compared to the single extract of each natural product (Experimental Example 1), and in suppressing inflammation of gingival cells It was confirmed that a synergistic effect was exhibited (Experimental Example 2).

Sancho ( Zanthoxylum schinifolium ), gold ( Scutellaria baicalensis ), and windbreak ( Peucedanum japonicum ) of the present invention can be directly collected or used by purchasing commercially available products, and the method of obtaining is not limited.

The snack composition for companion animals of the present invention comprises 100 parts by weight of meat, 90 to 110 parts by weight of fish, 40 to 60 parts by weight of seafood, and 40 to 60 parts by weight of the mixed extract, specifically, 100 parts by weight of meat, It may include 100 parts by weight of fish, 50 parts by weight of seafood, and 50 parts by weight of the mixed extract. In a specific embodiment of the present invention, when the mixed extract of sancho, gold, and windbreak is included in excess of 60 parts by weight, the snack intake rate of companion animals is significantly reduced. It was confirmed (Experimental Example 3). In addition, when the mixed extract is included in an amount of 40 parts by weight or less, antibacterial activity or anti-inflammatory activity against oral harmful bacteria may not be sufficiently exhibited.

The snack composition for companion animals of the present invention is characterized in that it has an effect of preventing stomatitis in companion animals. As used herein, the term “stomatitis” is a general term for inflammation in the oral cavity, and encompasses inflammation occurring in the oral mucosa of companion animals. It is known that the cause of stomatitis is usually caused by infections such as bacteria, viruses, and fungi. Since the snack composition of the present invention contains a natural extract, it has antibacterial activity against harmful oral bacteria and is effective in inhibiting inflammation of gingival cells, thereby helping to prevent stomatitis in companion animals.

The snack composition for companion animals of the present invention may further include additives commonly included in snacks for companion animals in the art, and specifically, a vitamin mixture including vitamin C and vitamin D, iron and copper It may further include a mineral mixture, a gelling agent such as agar, taurine, and the like. The content of the sub-additive may be determined in an amount generally formulated in the art, but is not limited thereto. However, the snack composition for companion animals of the present invention is characterized in that it does not contain preservatives, coloring agents, sweeteners, and seasonings.

Another aspect of the present invention provides a method for preparing the snack composition for companion animals. The manufacturing method of the snack composition for companion animals of the present invention includes preparing a mixed extract of wild pepper, gold, and windbreak, and mixing and processing the prepared mixed extract with raw materials of meat, fish, and seafood may include

Since the snack composition for companion animals of the present invention contains natural ingredients, it can help prevent stomatitis in companion animals by exhibiting high antibacterial and anti-inflammatory activity against harmful oral bacteria, and has excellent palatability, so it can be used as a health snack for companion animals. can

Hereinafter, the present invention will be described in more detail through examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Preparation Example 1. Preparation of wild pepper, gold, and windbreak extract

The effect on improvement of stomatitis was compared by preparing a mixed extract of the present invention, sancho, gold, and windbreak, and an extract of sancho, gold, or windbreak alone. Specifically, Sancho ( Zanthoxylum schinifolium ), gold ( Scutellaria baicalensis ), and windbreak ( Peucedanum japonicum ) After washing and drying, grinding was prepared. In the case of the mixed extract, Sancho, gold, and windbreak powder are mixed in a weight ratio of 1:1:1, distilled water 10 times the weight of the mixed powder is added, and extracted in hot water at 80 to 90° C. for 5 hours. A mixed extract of gold, and windbreak was prepared. In the case of a single extract of sancho, gold, or windbreak, distilled water of 10 times the weight of each powder was added and extracted in hot water at 80 to 90° C. for 5 hours to prepare each single extract. The extract was filtered using filter paper, concentrated under reduced pressure with a rotary vacuum concentrator, and dried with a freeze dryer to obtain a hot water extract and used as each sample.

Experimental Example 1. Antibacterial activity against oral harmful bacteria

Actinobacillus actinomycetemcomitans strain and Pasteurella multocida strain, which were found to be harmful bacteria in the oral cavity of cats (Veterinary Microbiology 148 (2011) 93-98), were cultured in a CO ₂ incubator for culturing under anaerobic and microaerobic conditions. For the antibacterial activity of the extract of the present invention against oral harmful bacteria, the minimum inhibition concentration (MIC) was measured using the broth dilution method.

Specifically, 100 μl of a culture solution pre-cultured for 24 to 48 hours in a liquid medium of each strain is inoculated into a brucella medium in which the concentration of each extract is 6.25, 12.5, 25, 50, 100 μg/ml added. After culturing for 24 to 72 hours, the growth degree of each bacteria was measured at 660 nm using a spectrophotometer to determine the minimum growth inhibitory concentration of the single extract and the mixed extract.

sample MIC (μg/ml) A. actinomycetemcomitans P. multocida Sancho Sole Extract - 100 Golden Sole Extract 50 100 Windbreak alone extract - 50 sancho, gold, windbreak
mixed extract 25 12.5

As shown in Table 1 above, the extracts of sancho, gold, or windbreak alone did not exhibit antibacterial activity at a concentration of 100 μg/ml or less, or showed a minimum growth inhibitory concentration of 50 or 100 μg/ml. In comparison, in the case of the mixed extracts of sancho, gold, and windbreak , it was confirmed that A. actinomycetemcomitans showed excellent antibacterial activity against oral harmful bacteria at 25 μg/ml and P. multocida at 12.5 μg/ml.

In other words, it was found that the mixed extract of sancho, gold, and windbreak of the present invention has synergistic activity compared to the extract of each natural product alone in terms of antibacterial activity against harmful bacteria in the oral cavity of cats.

Experimental Example 2. Inhibitory activity of inflammatory cytokines

In order to confirm the effect of the extract of the present invention on oral inflammation, the inhibitory effect of inflammatory cytokines on cells induced in vitro was measured. Specifically, HGF-1 (human gingival fibroblast) gingival cells were inoculated into a 24-well plate at a concentration of ^{1×10 6} cells/well, and cultured in a _{5% CO 2 incubator.} Thereafter, inflammation was induced by treatment with 1 μg/ml of Pg-LPS, and each extract was treated at a concentration of 150 μg/ml. After culturing for 24 hours, the expression levels of inflammatory cytokines IL-6 and IL-8 in the supernatant obtained by centrifuging the culture medium were quantified using an ELISA kit.

IL-6 expression level
(pg/ml) IL-8 expression level
(pg/ml) negative control 5.7±0.04 11.9 ± 1.29 positive control 275.1±5.79 408.2±7.82 Sancho Sole Extract 187.4±4.66 378.9±3.01 Golden Sole Extract 172.0±0.94 359.5±2.82 Windbreak alone extract 216.5±9.24 371.4±5.18 sancho, gold, windbreak
mixed extract 126.8±3.07 322.6±10.04

As a result, as shown in Table 2 above, when Pg-LPS was treated, the expression levels of interleukin-6 and interleukin-8, which are inflammatory cytokines, were significantly increased, but the expression of the mixed extracts of sancho, gold, and windbreak was treated It was confirmed that the amount was suppressed. In particular, the expression levels of IL-6 and IL-8 due to LPS induction were further significantly reduced when the mixed extract was treated, compared to the case of treating the single extract of sancho, gold, or windbreak, through which the single extract of each natural product In comparison, it was found that the mixed extracts of sancho, gold, and windbreak had a synergistic activity in suppressing inflammation of gingival cells.

Preparation Example 3. Preparation of a snack composition for companion animals

The snack composition for companion animals of the present invention was prepared at the compounding ratio of Table 3 below.

parts by weight Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 meat (chicken) 100 100 100 100 fish (salmon) 100 100 100 100 Seafood (crab meat) 50 50 50 50 mixed extract 50 0 30 70 Additives (vitamin mixture, mineral mixture, and taurine) 2.5 2.5 2.5 2.5

Typically, a snack for companion animals was prepared, including 100 parts by weight of chicken, 100 parts by weight of salmon, and 50 parts by weight of crab meat.

Experimental Example 3. Preference evaluation

In order to evaluate the palatability of the snack composition for companion animals of the present invention, the snack compositions of Examples and Comparative Examples were placed in a bowl and fed to the cats. Specifically, 20 cats were fasted for the night before the test, and 50 g of the prepared snack compositions of Examples and Comparative Examples were placed in each bowl, and each cat was placed in a designated space. After feeding the snacks for 1 hour, the amount of intake and the average intake rate were calculated by checking the amount of snacks remaining in the container.

division Example 1 Comparative Example 1 Comparative Example 2 Comparative Example 3 snack intake 45.9g 47.1g 46.3g 34.9g Average intake rate 91.8% 94.2% 92.6% 69.8%

As shown in Table 4, the composition of Example 1 containing 70 parts by weight of each of the mixed extracts of sancho, gold, and windbreak showed a degree of preference similar to that of the composition of Comparative Example 1 that did not contain the mixed extract by showing a degree of preference of 90% or more. confirmed that. On the other hand, it was confirmed that the composition of Comparative Example 3 containing 70 parts by weight of the mixed extract had a significantly lowered degree of preference to about 69%. As a result, it was found that the preference of cats decreased. In addition, in the case of the composition of Comparative Example 2 including the mixed extract in a relatively low content, it can be expected that the antibacterial activity or anti-inflammatory activity against stomatitis will be lower than that of the composition of Example 1.

That is, it was found that the feed composition for companion animals of the present invention contains a natural mixed extract, thereby exhibiting antibacterial and anti-inflammatory activity against oral harmful bacteria, can help prevent stomatitis in companion animals, and its preference is also excellent. .

From the above description, those skilled in the art to which the present invention pertains will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential characteristics thereof. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention should be construed as being included in the scope of the present invention, rather than the above detailed description, all changes or modifications derived from the meaning and scope of the claims described below and their equivalents.

Claims (5)

100 parts by weight of at least one meat selected from the group consisting of chicken, beef, and duck;
90 to 110 parts by weight of one or more fish selected from the group consisting of salmon, red snapper, and tuna;
40 to 60 parts by weight of at least one seafood selected from the group consisting of crab meat, squid, scallops, and shrimp; and
Comprising 40 to 60 parts by weight of a mixed extract obtained by mixing sancho, gold, and windbreak powder in a weight ratio of 1:1:1 and hot water extraction at 80 to 90° C. for 4 to 6 hours,
Comprising the mixed extract to be 16 to 17% by weight of the total weight of the companion animal snack composition,
A snack composition for companion animals, characterized in that it has an effect of preventing stomatitis in companion animals. delete delete delete delete