KR102301696B1 - Cosmetics composition with the extract of Aloe ferox for the improvement of sensitive or trouble skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for alleviating sensitive skin or skin problems, containing an aloe ferox extract as an active ingredient. A cosmetic composition of the present invention prevents skin problems and exhibits excellent effects of skin soothing, inflammation alleviation, and stimulation alleviation.

Description

Cosmetic composition with the extract of Aloe ferox for the improvement of sensitive or trouble skin containing aloe ferox extract as an active ingredient

The present invention relates to a cosmetic composition for improving sensitive skin and skin troubles containing an aloeperox extract as an active ingredient, and to a cosmetic composition effective for preventing skin troubles, soothing skin, alleviating skin inflammation, and alleviating skin irritation.

The skin is an organ that functions as a protective film that protects the living body from the external environment, and serves to prevent the loss of biocomponents such as water and electrolytes in the human body, and at the same time prevent harmful substances from entering the human body from the outside. The skin is largely divided into three layers: the epidermis, the dermis, and the subcutaneous tissue in order from the outside. Among them, the epidermal layer plays an important role in preventing the evaporation of moisture inside the human body.

Recently, it has been reported that when exposed to fine dust for a long period of time, immunity is rapidly lowered, which can lead to exposure to various diseases such as cardiovascular disease, skin disease, and eye disease, as well as respiratory diseases such as colds, asthma, and bronchitis. Fine dust is a very small substance that is invisible to our eyes. Unlike the nature of the dust, as artificial contaminants from fossil raw materials for use in factories, home, car, nitrate (NO ₃ ^-), ionic components such as ammonium (NH ₄ ^+), sulfate (SO ₄ ^2-) and It consists of carbon compounds, metal (elements) compounds, and the like.

Fine dust is up to 20 times smaller than the size of the pores, so it can easily penetrate into the skin. It can cause trouble or an inflammatory reaction. In addition, it can lead to serious diseases by weakening the immune function and losing the protective function of the skin barrier.

Due to the spread of yellow dust, fine dust and viruses, wearing a mask has become a daily necessity and has become a daily necessity. When the mask is in contact with the skin for a long time, acne bacteria are activated by creating an anaerobic environment inside the mask, and skin troubles occur as the skin is stimulated by the activation of sebum secretion due to the increase in skin humidity, and the skin friction of synthetic fibers and the mask. cases increased a lot.

Skin problems such as skin inflammation and acne are caused by a complex action of internal and external environmental causes such as fine dust, wearing a mask, and the influence of hormones, and more often occurs because sebum secretion is activated by wearing a mask. It causes chronic inflammation in the face, neck, and chest areas where a lot of sebum is secreted.

Inflammation causes many types of polynuclear leukocytes (PMNs) and an increase in immune substances, and these increased cells can treat and defend by secreting various types of proteolytic enzymes and cytokines, which are inflammatory cell products. let there be However, this action also causes detrimental damage to adjacent tissue cells and non-cellular components. Under appropriate conditions, normal function is restored after the initial state of inflammation has passed, but if the stimulant that stimulates inflammation does not disappear or is continuously made, chronic inflammation occurs as a result, causing serious tissue damage. Typical types of skin troubles related to inflammation include atopy and acne.

Sebum secreted from sebocytes and adipocytes prevents the penetration of harmful substances outside the skin and has a net function of regulating oil and moisture. However, when sebum is secreted excessively due to changes in puberty hormones, stress, etc., it causes dysfunction and various skin disorders. Hypersecretion of sebum is a disease of the sebaceous glands or skin troubles characterized by excessive secretion of sebum, which may also cause greasiness and seborrheic dermatitis.

Among the proteins that regulate sebum production, three transcription factors called PPARγ, SREBP-1, and C/EBPa play a key role. These three transcription factors induce the differentiation of sebaceous gland cells and interact with each other to generate sebum adjust the Among them, PPARγ promotes cell differentiation and plays an important role in regulating fatty acid metabolism, and is activated by polyunsaturated fatty acids including linoleic acid and 15-deoxy PGJ2. SREBP is known as a transcription factor that regulates the expression of genes involved in fatty acid and cholesterol metabolism, and is composed of three proteins: SREBP-1a, -1c, and -2. It is known that SREBP-1a and -1c are mainly involved in fatty acid metabolism and fat biosynthesis, and SREBP-2 is mainly involved in cholesterol homeostasis regulation. Studies on suppressing sebum secretion by regulating the activity of transcription factors that generate sebum are being actively conducted.

Korean Patent Registration No. 10-1477959 discloses a cosmetic composition for skin anti-irritation containing an aloe extract, etc., but is disclosed as having an anti-inflammatory effect by mixing with other extracts. Korean Patent Registration No. 10-1987719 discloses a cosmetic and pharmaceutical composition having excellent skin moisturizing, anti-inflammatory, and atopy improving effects, including aloe extract, etc., but is disclosed as having an effect by mixing, aloe The type of is not specified.

In the present invention, it is intended to develop and provide a cosmetic composition made of a natural material that is harmless to the human body and is effective in preventing skin troubles, soothing the skin, alleviating inflammation, and alleviating irritation.

The present invention relates to a cosmetic composition for improving sensitive skin and skin problems containing an aloe perox extract as an active ingredient.

In the cosmetic composition for improving sensitive skin and skin troubles of the present invention, the aloe perox extract may be an extract extracted using ethanol as an extraction solvent. In this case, the extraction is preferably ultrasonic extraction.

In the cosmetic composition for improving sensitive skin and skin troubles of the present invention, the aloe perox extract may preferably be included in an amount of 0.01 to 30.0% by weight based on the total weight of the cosmetic composition.

The cosmetic composition of the present invention prevents skin troubles and exhibits excellent effects in soothing skin, alleviating skin inflammation, and alleviating skin irritation. In addition, the cosmetic composition of the present invention is harmless to the human body by using a natural material and has excellent stability.

1 is a result confirming the cell regeneration effect of the present invention Aloe perox extract and Aloe vera extract.
Figure 2 is the result of confirming the inhibitory effect of NO (nitric oixde) production of the Aloe perox extract and Aloe vera extract of the present invention.
3 is a result confirming the sebum synthesis inhibitory effect of the Aloe perox extract and the Aloe vera extract of the present invention.

The present invention relates to a cosmetic composition for improving sensitive skin and skin problems containing an aloe perox extract as an active ingredient.

Aloe ferox used in the present invention is a perennial succulent plant in the family Liliaceae, native to the southeast of Cape State, South Africa. The leaves are lanceolate with a length of 40-60 cm and a width of 10-15 cm, and are colored light blue, often with thorns. The edge of the leaf has red thorns of keratin. It is a representative species of aloe that is propagated as a large species in real life and collects medicinal aloe extract. The word ferox of aloe ferox means rough in Latin, and it was named because of its thorns. As the name suggests, it is distinguished from the commonly used aloe vera in that it is a prickly aloe.

According to the experiment of the present invention, the Aloeperox extract of the present invention exhibited an excellent skin regeneration effect of 10 to 30 times more than that of Aloe vera. These results mean that the aloe vera extract of the present invention can restore skin damaged by skin troubles due to skin regeneration. Accordingly, the present invention may also be used for skin regeneration.

In addition, it was confirmed that the aloe perox extract showed superior anti-inflammatory effect than the aloe vera extract. This means that the composition of the present invention can be used for alleviating skin irritation caused by inflammation.

In addition, the aloe perox extract of the present invention exhibits a superior sebum synthesis inhibitory effect than the aloe vera extract. As a result, the aloe vera extract of the present invention inhibits sebum synthesis to suppress skin troubles caused by excessive sebum production. means This means that the composition of the present invention can be used for alleviating skin troubles caused by excessive sebum production.

In the cosmetic composition of the present invention, the Aloe perox extract may be extracted by an extraction method selected from the group consisting of a solvent extraction method, an ultra-high pressure extraction method, a supercritical extraction method, and an ultrasonic extraction method. An extraction method may be used. More preferably, it is good to use the ultrasonic extraction method.

The solvent extraction method is an anhydrous or hydrous lower alcohol having 1-4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof. An extraction solvent selected from may be used. Preferably, ethanol is used as a solvent.

The ultra-high pressure extraction method is preferably performed at a pressure of 100 to 3,000 MPa and a temperature of 40 to 90° C. for 20 to 30 hours, and treatment is performed by lowering the pH of the water inside the ultra high pressure device to 1 to 6.

The supercritical extraction method is an extraction method using a supercritical fluid, and preferably, carbon dioxide is used as the supercritical fluid.

The ultrasonic extraction method can be extracted by generating microbubbles by processing ultrasonic waves of 20-100 kHz at a temperature of 30-70° C. for 1-5 hours.

In the present invention, 70% (v/v) ethanol is preferably added to the dried aloeperox pulverized product, heated to 60° C. for 3 hours, extracted twice, filtered, concentrated under reduced pressure at 55° C., and frozen. Powdered Aloeferox extracts prepared by drying were used for each concentration.

Meanwhile, in the present invention, the Aloeferox extract is preferably contained in an amount of 0.01 to 30.0% by weight based on the total weight of the cosmetic composition of the present invention.

Hereinafter, the content of the present invention will be described in more detail in the following examples, but the scope of the present invention is not limited to the following examples, and includes modifications of technical ideas equivalent thereto.

[Example 1: Preparation of Aloe Perox Extract]

After adding 100 g of 70% (v/v) ethanol to 10 g of dried aloe perox, extraction was performed for 2 hours with an ultrasonic extractor at 40 kHz, and then cooled to room temperature. The cooling water obtained above was filtered with a filter paper, concentrated under reduced pressure, and freeze-dried to prepare a powdery Aloeperox extract by concentration and used in the following experiment.

[Comparative Example 1: Preparation of Aloe Vera Extract]

Except that Aloe vera was used instead of Aloe perox, Aloe vera extracts were obtained in the same manner as in Preparation Example 1, prepared by concentration, and used in the following experiments.

[Experimental Example 1: Cell regeneration effect]

200 μl of human-derived fibroblasts (HDFn) were dispensed in a 96 well plate at ^{1 x 10 4} cells/ml, and the cells were stabilized in a cell incubator at _{37° C., 5% CO 2 for one day.} Exchanged with DMEM medium containing 1% FBS, diluting Example 1 and Comparative Example 1 by concentration, and reacting in a cell incubator for 48 hours, then adding 5 mg/ml MTT reagent 20 μl to each well and incubated in a cell incubator for 1 to 2 hours. After removing all the supernatant and adding 100 μl of DMSO to each well to completely lyse the cells, absorbance was measured at 550 nm. Cell proliferation rate was calculated as a percentage of untreated cells. The results for this are shown in FIG. 1 .

As shown in FIG. 1 , the Aloe perox extract of Example 1 showed a skin regeneration effect of 15.2% (shown as 115.2% in FIG. 1) compared to the control at a concentration of 30 μg/ml, and the Aloe vera extract of Comparative Example 1 had a skin regeneration effect. At a concentration of 300 μg/ml, a skin regeneration effect of 5.5% (represented as 105.5% in FIG. 1 ) compared to the control group was exhibited. This is a result confirming that the aloe perox extract exhibits a skin regeneration effect that is 10 to 30 times greater than that of the aloe vera extract.

[Experimental Example 2: Inhibition of skin inflammation]

RAW 264.7 cells, which are mouse-derived macrophages, were seeded in a 96-well plate at 1×10 ⁵ cells/well, and then the cells were stabilized in a cell incubator at 37° C., 5% CO _{2 for one day.} After treatment with LPS (lipopolysaccharide) at a concentration of 1 μg/ml, Example 1 and Comparative Example 1 were diluted by concentration and treated with NDGA (nordihydroguaiaretic acid) as a positive control at a concentration of 5 uM. After reacting for 24 hours, 100 μl of the culture supernatant was separated, and 100 μl of Griess reagent was added in the same amount to react at room temperature, and then absorbance was measured at 540 nm using a microplate reader. The concentration of nitric oxide was calculated by comparing with a standard straight line obtained using sodium nitride (sodium nitrite, NaNO _{2 ).} The results for this are shown in FIG. 2 .

As shown in FIG. 2 , the LPS 1 μg/ml treated group (17.34 μM nitric oxide) significantly increased nitric oxide production 2.57 times compared to the untreated group (6.73 μM nitric oxide), and the aloepe of Example 1 When Rox extract was treated, nitric oxide production was inhibited in a concentration-dependent manner. At a concentration of 100 μg/ml of Aloeferox extract, a significant nitric oxide production inhibitory effect was shown, and at a concentration of 500 μg/ml, 14.90 μM of nitric oxide production showed a maximum inhibition of nitric oxide production of 23%. In the case of the Aloe vera extract of Comparative Example 1, there was no inhibitory effect on nitric oxide production up to a concentration of 500 μg/ml. This means that the aloe perox extract has a superior anti-inflammatory effect than the aloe vera extract.

[Experimental Example 3: Sebum synthesis inhibitory effect]

The effect on sebum production regulation transcription factors, PPAR-γ and SREBP-1, was confirmed to confirm the sebum production inhibitory effect.

Sebocytes were seeded in a 6-well plate at 3×10 ⁵ cells/well, and the cells were stabilized for one day at 37° C., 5% CO _{2 cell incubator.} After treatment with 200 μM linoleic acid, Example 1 and Comparative Example 1 were treated by concentration and reacted for 6 hours, and then cells were collected using a scraper. Proteins were isolated using 1 x cell lysis buffer, and proteins were quantified by BCA assay, and then a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. The separated protein on the gel was transferred to the PVDF membrane and blocked for 1 hour using 5% skim milk. After washing 3 times with 1 x TTBS solution, the PPAR-γ and SREBP-1 antibodies were reacted at room temperature for 2 hours. Thereafter, the band was detected with a Chemi Doc instrument after washing 3 times with 1 x TTBS and reacting the ECL solution. Beta-actin (β-actin) expression was quantified based on the expression level was calculated and quantified using Image J 1.47 software. The results are shown in FIG. 3 .

As a result of the experiment, the expression of PPAR-γ and SREBP-1 was significantly increased in the group treated with 200 μM of linoleic acid compared to the untreated group.

In the case of PPAR-γ, when the Aloeperox extract of Example 1 was treated, the expression of 47.57% PPAR-γ was significantly inhibited from a concentration of 300 μg/ml, and up to 100% or more of PPAR-γ at a concentration of 500 μg/ml was suppressed. When the Aloe vera extract of Comparative Example 1 was treated, the expression of 47.77% PPAR-γ was inhibited at a concentration of 500 μg/ml.

In the case of SREBP-1, when the Aloeperox extract of Example 1 was treated, the expression of 47.77% SREBP-1 was significantly inhibited from the concentration of 300 μg/ml, so that at the concentration of 500 μg/ml, the expression of up to 92.49% SREBP-1 was reduced. Expression was inhibited, and when the Aloe vera extract of Comparative Example 1 was treated, the expression of SREBP-1 was inhibited by 32.24% at a concentration of 300 μg/ml and 66.28% at a concentration of 500 μg/ml, respectively.

As a result, the Aloe perox extract showed a 2.62-fold and 1.44-fold higher inhibition of the expression of PPAR-γ, a sebum production regulator, and 1.44-fold higher, the expression of SREBP-1, compared to the Aloe vera extract. It means that it exhibits a more excellent sebum synthesis inhibitory effect.

[Formulation Example 1: Preparation of Cosmetic Composition Containing Aloe Perox Extract]

A cosmetic composition for improving sensitive skin and skin problems containing 0.1% by weight of the Aloeperox extract of Example 1 was prepared according to the composition shown in Table 1 below. However, the present invention can be applied in various formulations, and this formulation example is not intended to limit the present invention, but merely to describe it in detail.

The cosmetic composition of this formulation example was prepared according to the content of Table 1 below.

division ingredient Formulation Example 1
(weight%)
A Example 1 0.1 EDTA-2Na 0.02 Purified water to 100





B cetostearyl alcohol 2.0 Glyceryl Stearate 1.5 microcrystallin 0.7 squalane 5.0 liquid paraffin 3.0 trioctanoin 5.0 polysorbate 1.2 Sorbitan Stearate 0.5 Tocopheryl Acetate 0.2 cyclomethicone 3.0 BHT 0.05 C fragrance, preservative appropriate amount Sum 100

Claims (4)

Contains aloe ferrox ultrasonic extract as an active ingredient,
The aloe perox ultrasonic extract uses ethanol as an extraction solvent and is extracted by treating 20-100 kHz ultrasonic waves at 30-70° C. for 1-5 hours. Skin regeneration and sebum for improving sensitive skin and skin problems A cosmetic composition for inhibiting synthesis.
delete delete According to claim 1,
The aloe perox ultrasonic extract,
A cosmetic composition for skin regeneration and suppression of sebum synthesis for improving sensitive skin and skin problems, characterized in that it is contained in an amount of 0.01 to 30.0% by weight based on the total weight of the cosmetic composition.

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