KR102286199B1 - Pomegranate Juice with High Concentration of Ellagic Acid and Manufacturing Method Thereof - Google Patents

Abstract

The present invention relates to a pomegranate juice containing a high content of ellagic acid, which can prepare a beverage capable of exhibiting antioxidant and lipolytic effects and being nutritionally excellent by using a pomegranate having a high content of ellagic acid; and a method for preparing the same.

Description

Pomegranate Juice with High Concentration of Ellagic Acid and Manufacturing Method Thereof

The present invention relates to a pomegranate juice containing a high content of ellagic acid, which can exhibit antioxidant and lipolytic effects, and a nutritionally excellent beverage can be prepared by using a pomegranate having a high content of ellagic acid, and a method for preparing the same .

Pomegranate is one of two species of the pomegranate family, P. granatum and P. ptoropunica, and is a deciduous member belonging to the pomegranate family. Since ancient times, the dried fruit, stem, and root bark have been used extensively as herbal medicines. It is native to southwestern and northwestern India.

The succulent flesh of pomegranate is eaten as food, and in oriental medicine, the peel of the fruit is used as a medicine called pomegranate peel, which is used for diarrhea, dysentery, and anthelmintic. Since seed oil contains complex fatty acids, it is one of the most effective antioxidants that can be obtained from nature. It has the property of decomposing arteriosclerotic lesions and contains vitamin B1, which is good for motor and optic nerves, and B2, which is good for preventing diseases such as intellectual decline, memory loss, and Alzheimer's. In addition, pomegranate contains a large amount of tannins and phytoestrogens. Ellagic acid is a powerful antioxidant, and when used as an external preparation for the skin, it is effective in whitening and suppressing wrinkle formation. effects, including

However, the ingredients that directly affect the physiologically active functions listed above are known to be easy to destroy during the processing process, so it is difficult to manufacture or store a product that can exhibit a proper effect.

Republic of Korea Patent Registration No. 10-1692604

This invention is to meet the above technical problem, using pomegranate with a high content of ellagic acid, which can exhibit antioxidant and lipolytic effects, and can produce a nutritionally excellent beverage containing a high content of ellagic acid. An object of the present invention is to provide a pomegranate juice and a method for preparing the same.

In the constitution of this invention as a means for achieving the above object, 0.05 to 0.20 wt% of ellagic acid and 0.25 to 0.4 wt% of pomegranate-derived dietary fiber are contained.

In the configuration of the present invention, the pomegranate juice is prepared using the pomegranate pulp, which is subjected to steam treatment twice to increase the active ingredient by 15 to 20%.

In the configuration of the present invention, the pomegranate juice exhibits a lipolytic effect.

In the configuration of the present invention as a means for achieving the above object, the steps of washing the pomegranate; obtaining only the washed pulp of the pomegranate; juicing the pomegranate pulp using a juicer equipped with a twin screw; extracting pomegranate dietary fiber from the solids generated in the juicing step; and mixing the pomegranate dietary fiber in the step of extracting the pomegranate dietary fiber with the juice liquid in the juicing step.

In the configuration of the present invention, the step of obtaining only the pulp of the pomegranate includes a pre-treatment step, wherein the pre-treatment step includes: steaming the pomegranate pulp twice; When the steam treatment is completed, the step of treating ultrasonic waves;

In the configuration of the present invention, the extracting of the pomegranate dietary fiber includes: adding 300 mL of a solvent having a pH of 2 to 10 per 100 g of the solids generated in the juicing step and extracting for 2 to 4 hours; When the extraction is completed, filtration under reduced pressure to obtain an insoluble content; neutralizing the insoluble matter by immersing it in a lemon extract; washing the neutralized insoluble matter with distilled water; drying the washed insoluble matter; and pulverizing the dried insoluble matter to a particle size of 380 to 400 mesh.

In the configuration of the present invention, the mixing step includes a post-treatment step, and as the post-treatment step, drying and concentrating the mixture in the mixing step is performed.

In the configuration of the present invention, an additional enzyme treatment step may be included for color stabilization, and at least one of pectin lyase, pectinase, and glucoamylase is mixed with pomegranate juice and enzymatically treated in a buffer tank at a temperature of 20 to 30 ° C for 30 minutes to 1 hour.

This invention can be applied to the actual food industry, and by using a pomegranate with a high content of ellagic acid, it is possible to exhibit antioxidant and lipolytic effects and to prepare a nutritionally excellent beverage.

1 is a flowchart of a method for producing pomegranate juice according to the present invention.
2 is a flowchart of a pomegranate pretreatment step according to the present invention.
3 is a flowchart of the pomegranate dietary fiber extraction step according to the present invention.

Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the accompanying drawings in order to describe in detail enough that a person of ordinary skill in the art can easily carry out this invention. Other objects, including the object, action, effect, and operational advantages of this invention will become clearer by the description of the preferred embodiment.

For reference, the embodiments disclosed herein are only presented by selecting and presenting the most preferred embodiments in order to help those skilled in the art understand among various possible examples, and the technical spirit of the present invention is necessarily limited or limited only by the presented embodiments. It is not, and various changes, additions, and modifications including equivalents to substitutes are possible within the scope not departing from the technical spirit of the present invention.

In addition, the terms or expressions used in the specification and claims are defined on the basis of the principle that the inventor can appropriately define the concept of a term in order to best describe his invention, and It should not be construed as being limited to a dictionary meaning, but should be interpreted as meaning and concept consistent with the technical idea of this invention. As an example, the singular expression includes the plural expression unless the context clearly dictates otherwise.

1 is a flowchart of a method for producing pomegranate juice according to the present invention, FIG. 2 is a flowchart of a pomegranate pretreatment step according to the present invention, and FIG. 3 is a flowchart of a pomegranate dietary fiber extraction step according to the present invention.

1 to 3, in the configuration of the method for producing pomegranate juice according to the present invention, washing the pomegranate (S10); obtaining only the washed pulp of the pomegranate (S20); Juicing the pomegranate pulp using a juicer equipped with a twin screw (S30); extracting pomegranate dietary fiber from the solids generated in the juicing step (S40); and mixing the pomegranate dietary fiber in the step of extracting the pomegranate dietary fiber with the juice in the juicing step (S50).

The step (S20) of obtaining only the pulp of the pomegranate includes a pre-treatment step (S200), and as the pre-treatment step, steam treatment of the pomegranate pulp twice (S201); When the steam treatment is completed, the ultrasonic treatment step (S202); is made including.

Through the pretreatment step (S200), it is possible to further increase the content of ellagic acid, an active ingredient of the pomegranate, by 15-20%. When the steaming is performed less than two times, although the active ingredient in the pomegranate increases by a small amount, the content contained in the juice becomes less than 0.15% by weight due to the juice yield, so that the antioxidant and lipolytic effects are reduced, and the When carried out, the content of the active ingredient did not increase any more, but rather a small amount was lost.

Ellagic acid is a plant-derived polyphenol compound involved in various cellular metabolic processes and is composed of four polyphenol rings. Pure ellagic acid is a bright yellow crystalline solid, soluble in ethanol, methanol, and acetone.

The step of steaming twice (S301) is performed for 10 to 20 seconds by steam spraying at 120 to 160°C. If the above conditions are exceeded, the steam treatment is not uniform or the pomegranate pulp is heated excessively, resulting in loss of active ingredients.

Extracting the pomegranate dietary fiber (S40) includes adding 300 mL of a solvent having a pH of 2 to 10 per 100 g of the solids generated in the juicing step (S30) and extracting for 2 to 4 hours (S401); When the extraction is completed, filtration under reduced pressure to obtain an insoluble content (S402); Neutralizing the insoluble content by immersing in the lemon extract (S403); washing the neutralized insolubles with distilled water (S404); drying the washed insoluble matter (S405); and pulverizing the dried insoluble matter to a particle size of 380 to 400 mesh (S406).

The solvent used in the step (S40) of extracting the pomegranate dietary fiber may be selected from the group consisting of an organic solvent containing ethanol and a mixed solvent of water, and preferably 80% ethanol is used. At this time, the extraction temperature is preferably 10 ~ 80 ℃, can be extracted for 2 to 4 hours. If the extraction temperature and extraction time are out of the range, extraction efficiency may decrease or a change in components may occur.

The pomegranate juice in the mixing step (S50) contains 0.05 to 0.2 wt% of ellagic acid and 0.25 to 0.4 wt% of pomegranate-derived dietary fiber. More preferably, 0.15 to 0.21% by weight of ellagic acid and 0.25 to 0.4% by weight of pomegranate-derived dietary fiber are contained.

In the mixing step (S50), by mixing the pomegranate-derived dietary fiber, the consumer can increase the feeling of satiety and improve bowel activity.

In the method for preparing pomegranate juice of the present invention, an additional enzyme treatment step may be included to stabilize color development.

At least one of pectin lyase, pectinase, and glucoamylase is used. Pomegranate juice and enzyme are mixed in a buffer tank and enzymatically treated at 20-30°C for 30 minutes to 1 hour. In this case, when the enzyme treatment temperature is out of the range of 20 to 30° C., the activity of the enzyme is lowered, making it difficult to exhibit sufficient color stability effect.

Using the manufacturing method according to the present invention, pomegranate juice containing the steam treatment conditions and active ingredient concentrations shown in Table 1 below was prepared.

active ingredient Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Number of steam treatments 2 2 2 2 - One One 2 Steam processing time (seconds) 10 10 20 20 - 10 20 30 Ellagic acid (wt%) 0.15 0.18 0.19 0.21 - 0.09 0.10 0.20

* Comparative Example 1: Using distilled water

* Comparative Example 4: Steam treatment time exceeding 20 seconds

(Experimental Example 1) lipolysis effect

In order to confirm the lipolytic effect of the pomegranate juice according to the present invention, pomegranate juice (Examples 1-4, Comparative Examples 1-4) was prepared, and an experiment to confirm reduction in abdominal circumference was performed.

Adult males and females with a body fat percentage of 30% or more were divided into 3 subjects per group. Each group was instructed to drink 200mL of pomegranate juice instead of breakfast for 12 weeks, and to refrain from exercise during the experiment to confirm the fat-dissolving effect of pomegranate juice. The percentage of body fat increase and decrease was calculated by measuring InBody before and after 12 weeks. It is shown in Table 2 below.

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 0 parking
body fat
(kg) 21.2
±1.1 22.1
±1.0 23.5
±0.5 19.1
±1.9 21.4
±1.3 22.2
±0.7 24.0
±1.1 23.1
±1.4 Week 12
body fat
(kg) 20.1
±0.2 20.9
±0.5 20.9
±1.0 16.5
±0.3 20.9
±0.1 22.0
±0.4 23.8
±1.2 22.4
±0.4 Change (%) -5.2 -5.5 -12 -14 -2.4 -One -0.9 -3.1

As shown in the above results, body fat decreased in all groups, but among them, Examples 1 to 4 showed the highest body fat reduction rate. In Comparative Example 1, in which distilled water was drank instead of pomegranate juice, the body fat reduction effect was exhibited at a smaller rate than in Example. On the other hand, in Comparative Examples 2 and 3, which contained less ellagic acid than in Examples, the body fat reduction rate was less than that of Comparative Example 1, which drank distilled water, which is judged to be due to the caloric effect of the pomegranate juice itself. Comparative Example 4, which had the longest steam treatment time, had a higher body fat reduction rate than Comparative Example 1, but it was evaluated that it was difficult to drink because of the bitter taste. Therefore, the pomegranate juice prepared under the steam treatment conditions of the present invention is easy to drink and exhibits a fat decomposition effect.

Claims (8)

The process of washing pomegranates; a process of obtaining only the washed pomegranate flesh; The process of steaming the pomegranate flesh twice; When the steam treatment is completed, the process of processing with ultrasonic waves; Juicing the pomegranate pulp using a juicer equipped with a twin screw; The process of extracting for 2 to 4 hours by adding 300 mL of a solvent having a pH of 2 to 10 per 100 g of the solids generated in the juicing step; When the extraction is completed, the process of filtration under reduced pressure to obtain an insoluble matter; Neutralizing the insoluble matter by immersing it in a lemon extract; washing the neutralized insoluble matter with distilled water; drying the washed insoluble matter; The process of pulverizing the dried insoluble matter to a particle size of 380 to 400 mesh; including,
A process of preparing pomegranate juice by mixing the juice extracted in the juicing process and the crushed insoluble matter; For color stabilization, at least one of pectin lyase, pectinase, and glucoamylase is mixed with the pomegranate juice and heated in a buffer tank at a temperature of 20 to 30° C. for 30 minutes to 1 hour. It is manufactured through a process of enzymatic treatment.
Contains 0.05-0.2 wt% of ellagic acid, 0.25-0.4 wt% of pomegranate-derived dietary fiber,
Manufactured using pomegranate flesh with 15-20% increased active ingredient by steaming twice.
break down fat,
A pomegranate juice containing a high content of ellagic acid, characterized in that the color development is stabilized using at least one of pectinlyase, pectinase, and glucoamylase. delete delete delete washing the pomegranate;
obtaining only the washed pulp of the pomegranate;
steaming the pomegranate flesh twice;
processing with ultrasonic waves when the steam treatment is completed;
juicing the pomegranate pulp using a juicer equipped with a twin screw;
extracting for 2 to 4 hours by adding 300 mL of a solvent having a pH of 2 to 10 per 100 g of the solids generated in the juicing step;
When the extraction is completed, filtration under reduced pressure to obtain an insoluble content;
neutralizing the insoluble matter by immersing it in a lemon extract;
washing the neutralized insoluble matter with distilled water;
drying the washed insoluble matter;
Grinding the dried insoluble matter to a particle size of 380 to 400 mesh; including,
preparing pomegranate juice by mixing the juice extracted in the juicing process and the pulverized insoluble matter;
For color stabilization, at least one of pectin lyase, pectinase, and glucoamylase is mixed with the pomegranate juice and heated in a buffer tank at a temperature of 20 to 30° C. for 30 minutes to 1 hour. A method for producing pomegranate juice containing a high content of ellagic acid, characterized in that it comprises the step of enzymatic treatment during the process.
delete delete delete

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