KR102285828B1 - Cosmetic composition containing skin strengthening composition and method for producing same - Google Patents

Abstract

The present invention prepares a skin-enhancing cocktail containing Centella Asiatica, retinol, ceramide and panthenol as main ingredients, and then applies it to a cosmetic formulation in the form of liposomes, thereby strengthening skin tensile strength , collagen synthesis, GAG synthesis, skin thickness increase effect and to a cosmetic composition containing a skin strengthening composition to have a moisturizing effect, and a method for producing the same.

Description

Cosmetic composition containing skin strengthening cocktail and its manufacturing method {Cosmetic composition containing skin strengthening composition and method for producing same}

The present invention relates to a method for preparing a cosmetic containing a skin strengthening cocktail, and more particularly, a skin strengthening cocktail containing Centella Asiatica, retinol, ceramide and Panthenol as main ingredients. It relates to a cosmetic composition containing a skin-strengthening cocktail and a method for preparing the same, by applying it in the form of liposomes to a cosmetic formulation after preparing it to have the effect of strengthening skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase, and moisturizing effect. .

The skin undergoes physiological and structural aging due to factors of intrinsic aging and extrinsic aging. Physiological and structural symptoms caused by aging include thinning of the skin, loss of elasticity, sagging of the skin, and the appearance of wrinkles. In addition, a phenomenon in which the skin surface becomes rough is also accompanied. This phenomenon is known as a phenomenon that occurs when skin elastic fibers and collagen are damaged or the homeostasis of skin cell proliferation and differentiation is disrupted.

On the other hand, in modern society, many people, regardless of age or gender, use cosmetics. Among the cosmetic ingredients, retinoids, vitamins, and adenosin (Adenosin) are used as raw materials for various functional products for the purpose of removing dead skin cells and improving and preventing skin wrinkles. ), etc. have been used, but have been individually used to maintain skin homeostasis and inhibit skin aging. The reason is that there are many limitations in including one type of cosmetic composition due to the characteristics of ingredients that can act on each stage of skin aging. In addition, there is a problem that various functional ingredients do not match a specific ingredient ratio, and when the maximum content is used to increase the maximum effect, skin irritation or synergy between these ingredients cannot be created.

Therefore, there is a trend in active research on cosmetic compositions having skin tensile strength strengthening, collagen synthesis, GAG synthesis, skin thickness increase effect, moisturizing effect, and the like.

However, the conventional cosmetic composition had weak skin tensile strength strengthening, collagen synthesis, GAG synthesis, skin thickness increase effect and moisturizing effect, and penetrated into the skin to enhance tensile strength, collagen synthesis, GAG synthesis, skin thickness increase effect and moisturizing effect. In the situation where it is difficult to properly apply the

Korean Patent Laid-Open Patent No. 10-2018-0001652 (published on Jan. 05, 2018), "Cosmeceutical composition for strengthening skin barrier containing extract or a fraction thereof as an active ingredient" Republic of Korea Patent 10-1885225 (2018.08.02. Announcement), "Cosmeceutical composition containing mixed plant extracts to enhance skin elasticity, strengthen skin barrier, moisturize and contract pores"

In order to solve the above problems, the present invention prepares a skin-enhancing cocktail containing Centella Asiatica, retinol, ceramide and Panthenol as main ingredients, and then it is added to a cosmetic formulation in the form of liposomes. An object of the present invention is to provide a cosmetic composition containing a skin-enhancing cocktail and a method for manufacturing the same, which have the effect of strengthening skin tensile strength, collagen synthesis, GAG synthesis, increasing skin thickness, and moisturizing effect.

In order to solve the above problems, the present invention comprises the steps of mixing dried Centella asiatica powder with fermented alcohol (S10); Aging the mixture of step (S10) at room temperature (S20); Filtering the aged mixture of the step (S20) to prepare an extract (S30); Concentrating the extract of the step (S30) and then drying to prepare a dried Centella asiatica powder (S40); Preparing a skin strengthening cocktail by mixing the dried Centella asiatica powder of the step (S40) with retinol, ceramide and panthenol (S50); By including the step (S60) of collecting the skin strengthening cocktail of the step (S50) in liposomes and containing it in the cosmetic formulation; It provides a method for producing a cosmetic composition containing a skin strengthening cocktail.

In addition, in the step (S50), dried centella asiatica powder, retinol, ceramide, and panthenol are each mixed in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5.

In addition, the liposome collection of the skin-enhancing cocktail in the step (S60) is characterized in that it is made through a high-pressure homogenizer.

In addition, the skin strengthening cocktail collected in the liposome is characterized in that 0.5 to 5% by weight is included in the cosmetic formulation.

Manufactured by the above manufacturing method, it is characterized in that it has the effect of strengthening skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase, and moisturizing effect.

The cosmetic composition containing the skin strengthening cocktail prepared according to the manufacturing method of the present invention is a skin strengthening cocktail containing Centella Asiatica, retinol, ceramide and panthenol as main ingredients. Then, by applying it to the cosmetic formulation in the form of liposomes, it has the effect of strengthening skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase, and moisturizing effect.

1 is a flowchart showing a manufacturing process of a cosmetic composition containing a skin strengthening cocktail according to the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following detailed description of the present invention is an embodiment in which the present invention may be practiced, and reference is made to the accompanying drawings shown by way of example of the embodiment. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that various embodiments of the present invention are different but need not be mutually exclusive. For example, certain shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the invention in relation to one embodiment. In addition, it should be understood that the position or arrangement of individual components in each described embodiment may be changed without departing from the spirit and scope of the present invention.

Accordingly, the detailed description set forth below is not intended to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all scopes equivalent to those claimed by the claims. Like reference numerals in the drawings refer to the same or similar functions throughout the various aspects.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. In addition, in a specific case, there is a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the corresponding invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the present invention, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, a cosmetic composition containing a skin strengthening cocktail according to the present invention and a manufacturing method thereof will be described in detail.

The manufacturing method of the cosmetic composition containing the skin strengthening cocktail according to the present invention,

mixing the dried Centella asiatica powder with the fermented alcohol (S10);

Aging the mixture of step (S10) at room temperature (S20);

Filtering the aged mixture of the step (S20) to prepare an extract (S30);

Concentrating the extract of the step (S30) and then drying to prepare a dried Centella asiatica powder (S40);

Preparing a skin strengthening cocktail by mixing the dried Centella asiatica powder and retinol, ceramide and panthenol of the step (S40) in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, respectively (S50);

The skin strengthening cocktail of the step (S50) is captured in liposomes with a high pressure homogenizer in the cosmetic formulation and contained in an amount of 0.5 to 5% by weight (S60); by including; strengthening skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase To have an effect and moisturizing effect.

In the step (S10), the dried centella asiatica powder is mixed with the fermented alcohol.

The fermented alcohol is a vegetable alcohol, and it is appropriate to use 70% of it.

The dried Centella asiatica powder is mixed with 100 parts by weight of the fermented alcohol, and 25 to 50% by weight of the Centella asiatica powder to be mixed will be appropriate.

The dried Centella asiatica powder refers to dried and pulverized Centella Asiatica.

Centella Asiatica is a perennial herb commonly found in the mountains or fields of the southern islands of the Korean Peninsula. The main stem extends laterally, and there are two degenerated scaly leaves near the root node. Leaves are dense at nodes, elongate, 2-5cm in diameter, glossy on the surface, with dull serrated edges, and the length of petiole is 4-20cm. Flowers are reddish-purple, hung in umbels by 3-4 in leaf axils, and are shaped like a head. The style is bifurcated, and the fruit is a segmental oblong shape.

In the step (S10), the mixing of the fermented alcohol and the dried centella asiatica is made through a stirrer, and it will be appropriate to be performed at a speed of 50 to 150 rpm.

Next, a step (S20) of aging the mixture of step (S10) at room temperature is performed.

The aging of the step (S20) is appropriate to proceed for 48 to 96 hours, most suitably 72 hours, that is, it will be preferable to proceed for 3 days.

The effect of enhancing skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase and moisturizing effect of the cosmetic composition containing the skin strengthening composition of the present invention through the aging process of the step (S20) can be described. will be.

Next, the step (S30) of preparing an extract by filtering the aged mixture of the step (S20) is performed.

The filtration in the step (S30) is preferably performed through a filter paper having a transmission size of 5 to 10㎛.

The permeation size of the filter paper, 5 ~ 10㎛, filters impurities to maximize the skin tensile strength strengthening, collagen synthesis, GAG synthesis, skin thickness increase effect and moisturizing effect of the cosmetic composition containing the skin strengthening composition of the present invention. This is the optimal transmission size for obtaining the necessary components.

Next, the extract of step (S30) is concentrated and dried to prepare a dried centella asiatica powder (S40).

The drying of the step (S40) may be performed at a temperature of 50 to 80° C. using a dryer, and it may take a long time even if it is left at room temperature, but it is okay.

Next, mixing the dried centella asiatica powder, retinol, ceramide and panthenol of the step (S40) in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, respectively, to prepare a skin-enhancing cocktail (S50) carry out

The retinol is converted into retinal and retinoic acid in the living body, and since several derivatives are found and synthesized in the living body, they are collectively called retinol, and in the present invention, retinol is all of them. used as a denoting term. The retinol has an effect of promoting the biosynthesis of collagen (collagen fibers) essential for normal cell differentiation and growth induction of bones, teeth, hair, nails, and the like, and elastin (elastic fibers) that affect elasticity. Retinol promotes the metabolism of skin cells, normalizes cell differentiation, improves skin structure and elasticity, reduces wrinkles, and prevents dullness and roughness of the skin, thereby improving skin elasticity.

First, as a part of retinol is converted to retinoic acid, it penetrates into the skin and acts inside the skin, preventing damage and degeneration of collagen and elastic fibers in the skin dermis and promoting the production, making the skin elastic and suppressing wrinkles. .

Second, for the epidermis, it is reported that the skin cycle is returned to normal and the hydration of keratinocytes is increased to lighten the blemishes, and it is reported that it is effective in acne, psoriasis, keratin abnormalities, etc., and the photoaging recovery effect by UV is also reported. .

Despite these effects, due to the instability of retinol itself, it is an important task to stabilize retinol in the formulation and at the same time set it to an appropriate concentration in terms of stability.

The ceramide (Ceramide) is a lipid, a component present in a high content on the skin surface. The ceramide is already distributed in the market, it can be obtained by purchasing it.

On the other hand, the structure of the stratum corneum of the skin is very important for exerting the skin's inherent moisturizing and protective function. In particular, the lipid between keratinocytes forms a lamellar structure between the keratinized cells and acts like a hard cement, which is the skin barrier function. has become the source of The keratinocyte intercellular lipid is composed of lipid components such as ceramide, cholesterol, and fatty acids. Among them, the ceramide is a very important component that accounts for about 50% or more of the total amount, and provides a skin barrier function, a more fundamental lipid It can be called an ingredient. This stratum corneum is composed of protein-rich keratinocytes and the lipids filling in between them, and the structure of the stratum corneum has been described as a "two compartment model", It has been suggested and called "brick and mortar model" by analogy with the adhesive mortar. If keratinocytes are called bricks, lipid cells are the plaster (mortar) that firmly binds bricks. In addition, ceramide forms a lamellar liquid crystal structure together with cholesterol and fatty acids to create a strong structure that exhibits the skin barrier function. Therefore, when there is an abnormality in ceramide, the barrier function is weakened, and the inherent function of the skin is also deteriorated. As a result, various skin problems or skin diseases may occur or skin symptoms may be weakened. Ceramide is first, protects the skin's internal cells, prevents the penetration of external harmful substances, prevents transdermal moisture loss, second, inhibits the evaporation of moisture from the skin, and third, itchy skin, sensitive, infant skin, specific It is also known to be good for preventing skin diseases caused by dryness, such as allergic skin, which exhibits mild dryness due to changes in the environment due to the constitution.

The panthenol (Panthenol) refers to all kinds of panthenol, including dexpanthenol and DL-panthenol (DL-Panthenol).

The dexpanthenol is a derivative of pantothenic acid, which is used as a supplement for the treatment of skin diseases such as wounds and diaper rash, or is a drug used for the supply of pantothenic acid. Pantothenic acid is another name for vitamin B5, and dexpanthenol is broken down in the body and converted to pantothenic acid. In the case of dexpanthenol external application, it is used for skin regeneration and protection, moisturizing and anti-inflammatory purposes, and injections and syrups are used to supply pantothenic acid to the body.

The DL-panthenol refers to a mixed component of D-panthenol and L-panthenol. L-panthenol is an optical isomer of D-panthenol, and DL-panthenol is an optically inactive substance with a racemic structure in which the two are mixed by 50% each, and the optical isomer having right rotation and the optical isomer having left rotation are in the same amount. say

The panthenol has blood circulation and capillary dilatation functions.

In the present invention, when the dried Centella asiatica powder, retinol and ceramide act on the skin, the skin's metabolism becomes active, and the skin cells undergo proliferation and differentiation. At this time, for metabolism, nutrients and signal substances necessary for the blood circulation must be delivered to the epidermal cells. Therefore, by including Panthenol, which has blood circulation and capillary dilatation functions in the skin strengthening cocktail, the cosmetic composition containing the skin strengthening cocktail of the present invention can enhance skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase effect, and moisturizing effect are increased.

In addition, the blood circulation and capillary dilatation effects of panthenol may also be applied to the cosmetic composition containing the skin strengthening cocktail of the present invention.

In the step (S50), the dried centella asiatica powder, retinol, ceramide and panthenol are mixed in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, respectively, and the mixing is performed at a speed of 50-150rpm using a stirrer. can be performed with

Purified water may be included as the remaining amount in the step (S50).

Next, the step (S60) of collecting the skin-enhancing cocktail of the step (S50) in the liposome with a high-pressure homogenizer in the cosmetic formulation and containing 0.5 to 5% by weight is performed. The cosmetic formulation contains phospholipids.

In the step (S60), Nanofluids Technology is introduced as a stable nutrient carrier and moisture carrier of an unstable physiologically active substance, and it is possible to capture the physiologically active substances without chemical bonding by using the similarity to the system of the biological membrane model structure. In addition, it can be said that it has the characteristics of a release rate control system because it has excellent selective permeability and delay in the skin. For this, stabilization of the formulation was induced by forming emulsified liposomes using a high-pressure homogenizer.

The High Pressing Homogenizer is a pulverizer that pulverizes solid substances such as emulsions or suspended solids into fine particles, and is composed of a plunger pump and a homogenizing block. In the pulverization process by the high-pressure homogenizer, the material to be pulverized is supplied to the valve by a plunger pump action, the material is dispersed through a narrow gap between homo nozzles at high speed, and the dispersed particles are It can be carried out by being pulverized by shearing and collides at right angles with an impact ring at the exit to make the particles dispersed by the impact more fine.

In the step (S50), it is characterized in that the skin-enhancing cocktail composition is captured inside the liposome. In other words, centella asiatica dried powder and retinol, ceramide and panthenol are present in the liposome in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, respectively.

In this case, the liposome may be a glycerosome. The glycerosome refers to an endoplasmic reticulum composed of phospholipids, glycerol and water.

When the cosmetic formulation is a cream, stearyl alcohol, stearic acid, cholesterol stearate, squalene, 2-octyldodecyl alcohol, polyoxyethylene alcohol ester, glyceryl monostearate, skin strengthening cocktail, propylene glycol and purified water may be included. There will be.

When the cosmetic formulation is a softening lotion, skin strengthening cocktail, glycerin, 1,3-butylene glycol, PEG1600, allantoin, EDTA-2Na, benzophenone-9, sodium hyaluronate, ethanol, octyldodeses-16, polysol Bait 20, preservatives, fragrances, colorants and distilled water may be included.

When the cosmetic formulation is an astringent lotion, skin strengthening cocktail, glycerin, 1,3-butylene glycol, allantoin, EDTA-2Na, benzophenone-9, sodium hyaluronate, ethanol, polysorbate 20, uchiha gel extract, Citric acid, preservatives, fragrances, colors and distilled water may be included.

When the cosmetic formulation is a nutritional lotion, skin strengthening cocktail, glyceryl stearate SE, stearyl alcohol, lanolin, polysorbate, sorbitan stearate, hydrogenated vegetable oil, mineral oil, squalane, trioctanoin, dimethicone, tocopherol acetate , carboxyvinyl polymer, glycerin, 1,3-butylene glycol, sodium hyaluronate, triethanolamine, preservatives, fragrance, colorant, and distilled water.

When the cosmetic formulation is a nutritional cream, skin strengthening cocktail, lipophilic monostearyl acid glycerin, stearic acid, beeswax, polysorbate, sorbitan stearate, hydrogenated vegetable oil, squalane, mineral oil, trioctanoin, dimethicone, glycerin, Triethanolamine, sodium hyaluronate, preservatives, fragrances, colorants and distilled water may be included.

The present invention is not limited to the types of cosmetic formulations that can be used, and may include all cosmetic formulations containing 0.5 to 5% by weight of skin-enhancing cocktail by using a high-pressure homogenizer in the cosmetic formulation to form liposomes.

The cosmetic composition containing the skin-enhancing cocktail prepared through the above process is

Centella asiatica dry powder and retinol, ceramide and panthenol each include liposomes in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, including liposomes, strengthening skin tensile strength, collagen synthesis, GAG synthesis, and skin thickness increase It is characterized in that it is a cosmetic composition having effects and moisturizing effects as well as blood circulation and capillary dilatation effects.

Hereinafter, the cosmetic composition containing the skin strengthening cocktail according to the present invention will be described in detail through Examples, Comparative Examples and Experimental Examples.

Example 1. Skin strengthening cocktail contained cosmetics Preparation of the composition

Step (S10): 40% by weight of dried Centella asiatica powder was mixed with 100 parts by weight of 70% fermented alcohol.

Step (S20): The mixture of step (S10) was aged at room temperature for 3 days.

Step (S30): The aged mixture of step (S20) was filtered through filter paper having a transmission size of 5 μm to prepare an extract.

Step (S40): The extract of step (S30) was concentrated and dried to prepare a dried centella asiatica powder.

Step (S50): A skin strengthening cocktail was prepared by mixing the dried Centella asiatica powder and retinol, ceramide and panthenol of the step (S40) in a weight ratio of 30: 0.3: 3: 3, respectively.

Step (S60): The skin-enhancing cocktail of the step (S50) was captured in liposomes with a high-pressure homogenizer in the cosmetic formulation and contained in an amount of 3.0% by weight.

Example 2 to 25. Different composition ratios of skin-enhancing cocktails cosmetics Preparation of the composition

Perform the same process as in Example 1,

The cosmetic compositions of Examples 2 to 25 were prepared by varying the composition ratio of the skin strengthening cocktail of step (S50).

At this time, the composition ratio of the skin strengthening cocktail applied in the step (S50) is shown in Table 1 below.

Centella asiatica dried powder
weight ratio of retinol
weight ratio of ceramide
weight ratio panthenol
weight ratio Example 2 5 0.3 3 3 Example 3 10 0.3 3 3 Example 4 20 0.3 3 3 Example 5 40 0.3 3 3 Example 6 50 0.3 3 3 Example 7 60 0.3 3 3 Example 8 30 0.05 3 3 Example 9 30 0.1 3 3 Example 10 30 0.2 3 3 Example 11 30 0.4 3 3 Example 12 30 0.5 3 3 Example 13 30 0.6 3 3 Example 14 30 0.3 0.5 3 Example 15 30 0.3 One 3 Example 16 30 0.3 2 3 Example 17 30 0.3 4 3 Example 18 30 0.3 5 3 Example 19 30 0.3 6 3 Example 20 30 0.3 3 0.5 Example 21 30 0.3 3 One Example 22 30 0.3 3 2 Example 23 30 0.3 3 4 Example 24 30 0.3 3 5 Example 25 30 0.3 3 6

Examples 2 to 7 are to find an appropriate composition ratio by varying the content of Centella asiatica dry powder, Examples 8 to 13 are to find an appropriate composition ratio by varying the content of retinol, and Examples 14 to 19 are the content of ceramide. This is to find an appropriate composition ratio by being different, and Examples 20 to 25 are to find an appropriate composition ratio by changing the content of panthenol.

comparative example 1. Skin strengthening cocktail composition liposome put in without forming Makeup Preparation of a medical composition

Perform the same process as in Example 1,

In step (S60), the skin strengthening cocktail was contained in an amount of 3.0% by weight without forming liposomes in the cosmetic formulation.

comparative example 2. liposome inside Centella asiatica dried powder only captured cosmetics Preparation of the composition

Perform the same process as in Example 1,

Retinol, ceramide and panthenol were excluded, and only dried Centella asiatica powder was collected in the cosmetic formulation with a high-pressure homogenizer and contained in liposomes at 3.0% by weight.

comparative example 3. liposome Only retinol is captured inside. cosmetics Preparation of the composition

Perform the same process as in Example 1,

Centella asiatica dried powder, ceramide, and panthenol were excluded, and only retinol was collected in the liposome with a high-pressure homogenizer in the cosmetic formulation, and contained at 3.0% by weight.

comparative example 4. liposome Only ceramides are captured inside. cosmetics Preparation of the composition

Perform the same process as in Example 1,

Centella asiatica dried powder, retinol and panthenol were excluded, and only ceramide was contained in a cosmetic formulation by forming liposomes with a high-pressure homogenizer in an amount of 3.0% by weight.

comparative example 5. liposome inside Panthenol only captured cosmetics Preparation of the composition

Centella asiatica dried powder, retinol and ceramide were excluded, and only panthenol was captured in the liposome with a high-pressure homogenizer in the cosmetic formulation and contained at 3.0% by weight.

Hereinafter, the cosmetic composition containing the skin strengthening cocktail of the present invention will be described through Experimental Examples 1 to 4.

The following Experimental Examples 1 to 4 were performed using artificial skin.

HDF (human dermal fibroblast) cells were used, and 1.0 Х 105 cells were prepared per 1 ml of the collagen gel solution. Culture medium was 10% Fetal Bovine Serum (FBS, Biowest) and 1% Penicillin-Streptomycin (P / S, 100X, Biowest) was added and was used, 37 ℃ 5% CO ₂ environment in Dulbecco Modified Eagle Medium (DMEM, Biowest ) was cultured in Examples 1 to 25 and Comparative Examples 1 to 5 were dissolved in DMS to a concentration of 1% in the culture solution and contained in the artificial skin culture solution. After changing the culture medium every 3 days, after 3 weeks of Example treatment, the following test items are compared with the untreated group (control group).

The result was shown using the following Equation (1).

Experimental example 1. Tensile strength measurement experiment

The mechanical properties of the manufactured artificial skin dermis were performed using a Universal Testing Machine (UTM, DrTech) device. The hydrogel for measuring the compressive strength was standardized to have a diameter of 8 mm and a height of 1 mm, and a total of three samples were prepared and measured for each. Tensile strength was measured by applying the same set values of the universal testing machine as measuring distance test ratio of 12%, test speed of 1.0 mm/min, and load range of 1.0 kgf. Table 2 shows the skin strengthening index with respect to tensile strength using Equation 1 above.

Skin Strengthening Index (%) for Tensile Strength Example 1 30 Example 2 15 Example 3 23 Example 4 28 Example 5 29 Example 6 24 Example 7 17 Example 8 14 Example 9 22 Example 10 27 Example 11 28 Example 12 24 Example 13 15 Example 14 17 Example 15 23 Example 16 28 Example 17 28 Example 18 24 Example 19 16 Example 20 14 Example 21 25 Example 22 29 Example 23 28 Example 24 23 Example 25 17 Comparative Example 1 18 Comparative Example 2 17 Comparative Example 3 16 Comparative Example 4 13 Comparative Example 5 0

As a result, from the results of Examples 1 to 25, an appropriate mixing ratio of the dried centella asiatica powder is 10 to 50 weight ratio, an appropriate mixing ratio of retinol is 0.1 to 0.5 weight ratio, an appropriate mixing ratio of ceramide is 1 to 5 weight ratio, and an appropriate mixing ratio of panthenol is It was confirmed that the ratio was 1 to 5 by weight, and Examples 2, 7, 8, 13, 14, 19, 20, and 25 were not suitable for use. appear.

From the above results, the cosmetic composition containing the skin strengthening cocktail according to the present invention is 10-50: 0.1-0.5: 1-5: When mixed in a weight ratio of 1-5, each of Centella asiatica dry powder, retinol, ceramide and panthenol, the skin It was confirmed that the tensile strength was strengthened by a minimum of 20% to a maximum of 30%.

In addition, compared to when the skin-enhancing cocktail is not captured in the liposome or when the Centella asiatica dry powder, retinol, ceramide or panthenol is collected alone in the liposome, it is more when captured in the liposome including all of the Centella asiatica dry powder, retinol, ceramide and panthenol. High skin tensile strength strengthening values were confirmed.

From Experimental Example 1, it could be inferred that an unexpected result occurred when collecting in liposomes including all of the dried Centella asiatica powder, retinol, ceramide and panthenol.

Experimental example 2. Artificial skin dermis pro - collagen detection test

Pro-collagen analysis was performed to confirm the artificial skin collagen synthesis rate. HDF cells were swollen and encapsulated in a porous collagen gel at a concentration of 5.0×10 ³ and gelled in a 48 well at 37° C., using DMEM culture medium with FBS and P/S added at 37° C., 5% CO ₂ environment. incubated for 1 day. After removing the culture medium, it was cultured for 1 day with the culture medium to which Examples and Comparative Examples were added, and used for pro-collagen synthesis rate analysis. In this experiment, adenosin at a concentration of 0.04% was selected as a positive control, and pure culture was analyzed as a control.

To check the collagen synthesis rate of Examples and Comparative Examples, HDF cells ^{were encapsulated in a collagen gel solution of 0% GTA at a concentration of 5.0×10 3} and gelled in 96 wells. The culture was carried out for 2 days, and the culture medium composition and culture environment were the same as in the above method. 400 ppm of adenosin was selected as a positive control, and pure culture was analyzed as a control.

The analysis of this experiment was performed with pro-collagen type 1 C-peptide (PIP) EIA kit (Takara). First, 11 ml of distilled water was added to the enclosed antibody-POD to prepare an antibody-POD solution. The cell culture solution and pro-collagen standard solution used for culture were mixed with 0.1 ml of antibody-POD solution in 0.02 ml each, placed in 96 wells coated with the enclosed antibody, and the reaction was performed at 37° C. for 3 hours. After removing the remaining solution after the reaction, it was washed 4 times with PBS to remove the remaining solution. 0.1ml of the enclosed substrate solution was added to stabilize at room temperature for 15 minutes, and 0.1ml of 1N sulfuric acid solution (stop solution) using distilled water as a solvent was added to stop the reaction. Then, absorbance was measured and analyzed at a wavelength of 450 nm using Elisa in 96 wells. Table 3 shows the skin strengthening index for pro-collagen synthesis using Equation 1 above.

Skin tightening index for pro-collagen synthesis (%) Example 1 46 Example 2 19 Example 3 35 Example 4 43 Example 5 45 Example 6 37 Example 7 22 Example 8 21 Example 9 35 Example 10 42 Example 11 44 Example 12 37 Example 13 23 Example 14 22 Example 15 36 Example 16 43 Example 17 45 Example 18 38 Example 19 24 Example 20 21 Example 21 34 Example 22 41 Example 23 45 Example 24 38 Example 25 20 Comparative Example 1 17 Comparative Example 2 16 Comparative Example 3 14 Comparative Example 4 13 Comparative Example 5 0

As a result, from the results of Examples 1 to 25, an appropriate mixing ratio of the dried centella asiatica powder is 10 to 50 weight ratio, an appropriate mixing ratio of retinol is 0.1 to 0.5 weight ratio, an appropriate mixing ratio of ceramide is 1 to 5 weight ratio, and an appropriate mixing ratio of panthenol is It was confirmed that the ratio was 1 to 5 by weight, and Examples 2, 7, 8, 13, 14, 19, 20, and 25 were not suitable for use. appear.

From the above results, the cosmetic composition containing the skin strengthening cocktail according to the present invention is 10-50: 0.1-0.5: 1-5: When mixed in a weight ratio of 1-5, each of Centella asiatica dry powder, retinol, ceramide and panthenol, pro It was confirmed that collagen synthesis was increased by a minimum of 34 to a maximum of 46%.

In addition, compared to when the skin strengthening cocktail is not captured in the liposome, or when the Centella asiatica dry powder, retinol, ceramide or panthenol is collected in the liposome alone, it is more High procollagen synthesis levels were confirmed.

From Experimental Example 2, it could be inferred that unexpected results occurred when collecting in liposomes including all of the dried Centella asiatica powder, retinol, ceramide and panthenol.

Experimental example 3. Dermal Cells GAG synthesis and secretion

GAG (glycosaminoglycan) synthesis and secretion were detected by Alcian blue staining to check the degree of metabolism of cells in the dermis of artificial skin by intensity. In order to prevent additional GAG synthesis of artificial skin cells cultured on the 7th day, the metabolic activity of the cells should be stopped. stopped metabolic activity. Thereafter, 0.1 ml of a 0.05M sodium acetate buffer (3M, Sigma) containing 0.05M MgCl2 (Sigma) was added to Alcian blue (Aladin) with a concentration of 0.05% (w/w) as a solvent and 0.1ml was treated at room temperature overnight. dyed. After removing the Alcian blue solution and washing the residual Alcian blue solution remaining on the artificial skin gel with PBS, an image was taken with an optical microscope. Table 4 shows the skin strengthening index for GAG synthesis using Equation 1 above.

Skin tightening index for GAG synthesis (%) Example 1 31 Example 2 11 Example 3 21 Example 4 29 Example 5 28 Example 6 22 Example 7 12 Example 8 9 Example 9 23 Example 10 30 Example 11 27 Example 12 22 Example 13 13 Example 14 11 Example 15 24 Example 16 28 Example 17 29 Example 18 22 Example 19 12 Example 20 8 Example 21 23 Example 22 30 Example 23 28 Example 24 25 Example 25 7 Comparative Example 1 6 Comparative Example 2 4 Comparative Example 3 3 Comparative Example 4 5 Comparative Example 5 0

As a result, from the results of Examples 1 to 25, an appropriate mixing ratio of the dried centella asiatica powder is 10 to 50 weight ratio, an appropriate mixing ratio of retinol is 0.1 to 0.5 weight ratio, an appropriate mixing ratio of ceramide is 1 to 5 weight ratio, and an appropriate mixing ratio of panthenol is It was confirmed that the ratio was 1 to 5 by weight, and Examples 2, 7, 8, 13, 14, 19, 20, and 25 were not suitable for use. appear.

From the above results, the cosmetic composition containing the skin-enhancing cocktail according to the present invention was mixed in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5, respectively, of Centella asiatica dry powder, retinol, ceramide and panthenol, GAG It was confirmed that the synthesis increased by a minimum of 21% and a maximum of 31%.

In addition, compared to when the skin strengthening cocktail is not captured in the liposome, or when the Centella asiatica dry powder, retinol, ceramide or panthenol is collected in the liposome alone, it is more A high GAG synthesis level was confirmed.

From Experimental Example 3, it could be inferred that unexpected results occurred when collecting in liposomes including all of the dried Centella asiatica powder, retinol, ceramide and panthenol.

Experimental example 4. Change in thickness of artificial skin

The artificial skin cultured on the 14th day was subjected to punch biopsy to make a 10 μm section, and after H&E staining, the thickness of the artificial skin was measured under a microscope. Table 5 shows the skin strengthening index with respect to the thickness change using Equation 1 above.

Skin tightening index with respect to thickness change (%) Example 1 39 Example 2 17 Example 3 23 Example 4 38 Example 5 37 Example 6 24 Example 7 16 Example 8 18 Example 9 25 Example 10 36 Example 11 38 Example 12 24 Example 13 14 Example 14 18 Example 15 28 Example 16 35 Example 17 37 Example 18 27 Example 19 19 Example 20 15 Example 21 24 Example 22 38 Example 23 36 Example 24 25 Example 25 12 Comparative Example 1 15 Comparative Example 2 12 Comparative Example 3 11 Comparative Example 4 13 Comparative Example 5 0

As a result, from the results of Examples 1 to 25, an appropriate mixing ratio of the dried centella asiatica powder is 10 to 50 weight ratio, an appropriate mixing ratio of retinol is 0.1 to 0.5 weight ratio, an appropriate mixing ratio of ceramide is 1 to 5 weight ratio, and an appropriate mixing ratio of panthenol is It was confirmed that the ratio was 1 to 5 by weight, and Examples 2, 7, 8, 13, 14, 19, 20, and 25 were not suitable for use. appear.

From the above results, the cosmetic composition containing the skin-enhancing cocktail according to the present invention is 10-50: 0.1-0.5: 1-5: When mixed in a weight ratio of 1-5, each of Centella asiatica dry powder, retinol, ceramide and panthenol, the skin It was confirmed that the thickness increased by a minimum of 23% to a maximum of 39%.

In addition, compared to when the skin-enhancing cocktail is not captured in the liposome or when the Centella asiatica dry powder, retinol, ceramide or panthenol is collected alone in the liposome, it is more when captured in the liposome including all of the Centella asiatica dry powder, retinol, ceramide and panthenol. High skin thickness increase was confirmed.

From Experimental Example 4, it could be inferred that unexpected results occurred when collecting in liposomes including all of the dried Centella asiatica powder, retinol, ceramide and panthenol.

Experimental example 5. cosmetics comparative test

Human experiments were conducted for Examples 1 to 25 and Comparative Examples 1 to 5. Each of Examples 1 to 25 and Comparative Examples 1 to 5 were prepared by including a certain amount in the cosmetic formulation of Table 6 below, and this was prepared on the right side of the face of 5 experimenters (females aged 20 to 35) twice a day for 1 month in a row. was applied. After completion of the experiment, skin wrinkles and dead skin cells were measured using an image analyzer for the skin on the left and right sides of the face.

ingredient content (wt%)
go stearin alcohol 8.0 stearic acid 2.0 cholesterol stearate 2.0 squalene 4.0 2-octyldodecyl alcohol 6.0 polyoxyethylene alcohol ester 3.0 Glyceryl monostearate ester 2.0
me Examples 1 to 25 or Comparative Examples 1 to 5 2.0 propylene glycol 5.0 Purified water remaining amount

- measurement method

Skin moisture content: Corneometerⓡ CM825

Transdermal water loss (TEWL): Tewameterⓡ TM300

Wrinkles around the eyes: Visual evaluation, PRIMOS lite (Ra, Rmax, Rz, Rp, Rv)

Skin elasticity: Ballistometer BLS780 (K, Indent, Alpha, Mean CoR, Area)

Skin roughness: PRIMOS lite (Ra, Rmax, Rz, Rp, Rv)

Experimental results were expressed using Equation 2 below.

The experimental results are as shown in Table 7 as the skin improvement rate.

moisture TEWL eye wrinkles skin elasticity skin roughness Skin improvement rate (%) of the cosmetic formulation containing Example 1 9.5 5.5 10.8 11.8 11.9 Skin improvement rate (%) of the cosmetic formulation containing Example 2 4.3 2.2 4.3 5.1 5.3 Skin improvement rate (%) of the cosmetic formulation containing Example 3 5.5 3.2 5.4 7.2 7.4 Skin improvement rate (%) of the cosmetic formulation containing Example 4 7.2 4.6 9.1 10.6 9.5 Skin improvement rate (%) of the cosmetic formulation containing Example 5 8.4 4.3 8.8 10.4 10. Skin improvement rate (%) of the cosmetic formulation containing Example 6 5.7 3.4 6.7 7.7 7.1 Skin improvement rate (%) of the cosmetic formulation containing Example 7 3.6 2.5 4.5 5.4 6.1 Skin improvement rate (%) of the cosmetic formulation containing Example 8 4.3 2.4 4.6 5.3 5.1 Skin improvement rate (%) of the cosmetic formulation containing Example 9 5.3 3.1 6.1 7.8 8.2 Skin improvement rate (%) of the cosmetic formulation containing Example 10 8.2 4.0 9.3 10.6 9.3 Skin improvement rate (%) of the cosmetic formulation containing Example 11 8.5 4.4 8.1 9.3 9.3 Skin improvement rate (%) of the cosmetic formulation containing Example 12 5.6 3.6 5.4 7.5 8.4 Skin improvement rate (%) of the cosmetic formulation containing Example 13 4.5 2.3 4.9 6.3 6.6 Skin improvement rate (%) of the cosmetic formulation containing Example 14 3.3 2.2 4.3 5.6 6.2 Skin improvement rate (%) of the cosmetic formulation containing Example 15 5.2 3.4 6.8 7.1 7.3 Skin improvement rate (%) of the cosmetic formulation containing Example 16 8.9 4.7 8.5 10.4 10.1 Skin improvement rate (%) of the cosmetic formulation containing Example 17 7.8 4.6 8.5 9.3 10.8 Skin improvement rate (%) of the cosmetic formulation containing Example 18 5.4 3.3 5.7 7.9 7.4 Skin improvement rate (%) of cosmetic formulations containing Example 19 3.6 2.5 4.8 6.9 5.5 Skin improvement rate (%) of the cosmetic formulation containing Example 20 3.7 2.4 4.3 6.1 5.3 Skin improvement rate (%) of the cosmetic formulation containing Example 21 6.1 3.1 6.6 7.2 7.1 Skin improvement rate (%) of the cosmetic formulation containing Example 22 7.1 4.5 9.4 10.3 9.6 Skin improvement rate (%) of the cosmetic formulation containing Example 23 7.4 4.3 9.5 10.4 10.4 Skin improvement rate (%) of cosmetic formulations containing Example 24 5.3 3.7 7.2 7.5 7.9 Skin improvement rate (%) of cosmetic formulations containing Example 25 4.5 2.3 4.8 5.1 5.2 Skin improvement rate (%) of cosmetic formulations containing Comparative Example 1 3.8 2.1 2.8 2.1 2.3 Skin improvement rate (%) of the cosmetic formulation containing Comparative Example 2 3.3 1.8 1.9 1.8 1.9 Skin improvement rate (%) of the cosmetic formulation containing Comparative Example 3 3.5 1.7 2.5 2.2 2.3 Skin improvement rate (%) of the cosmetic formulation containing Comparative Example 4 3.6 1.9 2.6 2.3 2.4 Skin improvement rate (%) of the cosmetic formulation containing Comparative Example 5 2.8 1.4 1.7 1.6 1.7

As a result, from the results of Examples 1 to 25, an appropriate mixing ratio of the dried centella asiatica powder is 10 to 50 weight ratio, an appropriate mixing ratio of retinol is 0.1 to 0.5 weight ratio, an appropriate mixing ratio of ceramide is 1 to 5 weight ratio, and an appropriate mixing ratio of panthenol is It was confirmed that the ratio was 1 to 5 by weight, and Examples 2, 7, 8, 13, 14, 19, 20, and 25 were not suitable for use. appear.

From the above results, the cosmetic composition containing the skin strengthening cocktail according to the present invention contains 10-50: 0.1-0.5: 1-5: 1-5 weight ratio of dried centella asiatica powder, retinol, ceramide and panthenol, respectively. , TEWL, wrinkles around the eyes, skin elasticity, and skin roughness were confirmed to show excellent skin improvement rates.

In addition, compared to when the skin-enhancing cocktail is not captured in the liposome or when the Centella asiatica dry powder, retinol, ceramide or panthenol is collected alone in the liposome, it is more when captured in the liposome including all of the Centella asiatica dry powder, retinol, ceramide and panthenol. It was possible to confirm the skin improvement rate for the evaluation items of high moisture, TEWL, wrinkles around the eyes, skin elasticity, and skin roughness.

From Experimental Example 5, it could be inferred that unexpected results occurred when collecting in liposomes including all of the dried Centella asiatica powder, retinol, ceramide and panthenol.

Although the present invention has been described with the accompanying drawings, this is only one embodiment of various embodiments including the gist of the present invention, and is intended to be easily implemented by those of ordinary skill in the art. For the purpose, it is clear that the present invention is not limited to the embodiments described above. Accordingly, the protection scope of the present invention should be construed by the following claims, and all technical ideas within the equivalent range by changes, substitutions, substitutions, etc. within the scope not departing from the gist of the present invention are in the right of the present invention. will be included In addition, it is clarified that some components of the drawings are provided in an exaggerated or reduced form than in reality for more clearly explaining the configuration.

Claims (5)

Mixing the dried centella asiatica powder with the fermented alcohol (S10);
Aging the mixture of step (S10) at room temperature (S20);
Filtering the aged mixture of the step (S20) to prepare an extract (S30);
Concentrating the extract of the step (S30) and then drying to prepare a dried Centella asiatica powder (S40);
Preparing a skin-enhancing cocktail by mixing the dried centella asiatica powder of the step (S40) with retinol, ceramide and panthenol (S50);
By including the step (S60) of collecting the skin strengthening cocktail of the step (S50) in the liposome and containing it in the cosmetic formulation;
In the step (S50), dry centella asiatica powder, retinol, ceramide and panthenol are each mixed in a weight ratio of 10-50: 0.1-0.5: 1-5: 1-5 of a cosmetic composition containing a skin strengthening cocktail. manufacturing method. delete According to claim 1,
The method for producing a cosmetic composition containing a skin strengthening cocktail, characterized in that the liposome collection of the skin strengthening cocktail in the step (S60) is achieved through a high-pressure homogenizer. According to claim 1,
A method for producing a cosmetic composition containing a skin strengthening cocktail, characterized in that the skin strengthening cocktail collected in the liposome is included in the cosmetic formulation in an amount of 0.5 to 5% by weight. A skin strengthening composition prepared by the manufacturing method of any one of claims 1, 3, and 4, characterized in that it has the effect of strengthening skin tensile strength, collagen synthesis, GAG synthesis, skin thickness increase and moisturizing effect. Contained cosmetic composition.

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