KR102273480B1 - Structure of transplanting seaweed for creation and recover marine forest - Google Patents

Abstract

The present invention relates to a seaweed transplantation structure in which an adhesive organ (rhizome) of Perennial sargassaceae seaweeds with a frond length of 10 to 30 cm is directly fixed on the surface of an attachment substrate with an adhesive, and a method for manufacturing the same. According to the present invention, the seaweed transplantation structure can effectively transplant Perennial sargassaceae seaweeds which are considered difficult to perform adult transplantation due to the small diameter of an adhesive organ and the excellent survival rate of the transplanted seaweeds was confirmed through on-site monitoring for several months. The Perennial sargassaceae seaweeds, which reproduce sexually by growing over several years and forming reproductive deposits every year, survive for several years through a single adult transplantation and serve as spore sacs producing and releasing spores every year, thereby saving material, manpower, and time and being useful for forming a marine forest. In addition, the seaweed transplantation structure is usefully applied to a seawater aquarium, whichs is an expanding market, to form an ecosystem in various tanks.

Description

Structure of transplanting seaweed for creation and recover marine forest

The present invention relates to a structure for transplanting seaweeds in which an attachment group (rhizome ) of perennial Sargassaceae seaweeds is fixed with an adhesive, a method for manufacturing the same, and a method for creating or restoring a marine forest using the same.

In Korea's subtidal 'natural marine forest', large brown algae such as Sargassum spp . and Ecklonia cava form an upper canopy, and Cladophora spp .), curly Small and medium-sized seaweeds such as Plocamium spp . and Jointed calcareous algae form a colony, and angular seaweeds such as Sonderophycus spp . and melobesioidean algae are close to the substrate. It consists of a structure that forms vegetation on the lowest layer. Moreover, many seaweed species that make up the upper and middle layers of the marine forest are not only habitats, shelters, spawning grounds , and food sources for coastal benthic and fish, but also Ceramium spp ., Neosiphonia spp.) It plays a role in enhancing the biodiversity of the entire marine ecosystem by serving as a substrate for small seaweed species, such as small seaweed species, or as a shade for a specific seaweed species sensitive to light intensity.

Recently, as a result of complex human activities, various harmful factors including industrial waste, nutrients, and sewage and wastewater flowing into the marine ecosystem, and physical and chemical disturbances in the coast caused by changes in the structure of the coastline are caused by the seaweed community. The decline continues to intensify. These phenomena are first of all, the loss of large brown algae forming the upper water tube of the algae community, the disappearance of the medium soft algae community forming the middle layer, the loss of the algae community, and the exposure and discoloration of the lower algae community. It leads to the so-called 'float greening or sea desertification phenomenon', and recently there is a report that sea desertification has progressed in more than 60% of the eastern coast of Korea and 30% of the Jeju-do and southern coasts.

The progress of tidal greening deteriorates the habitat environment of numerous creatures that feed, spawn, and inhabit the marine forest, and consequently, the species diversity and biomass of resource fish and shellfish as well as economically useful seaweed such as seaweed, algae, and kelp decrease. This is causing great damage to the fishermen who make it their means of livelihood. As a method to cope with this phenomenon of tidal greening, recovery of various types of marine forests, such as expansion of the seaweed attachment substrate area through artificial reef facilities for marine forests, induction of spore diffusion through installation of imitation pouches, induction of various seaweed transitions through swamping, and artificial seaweed transplantation and activities for the creation of

Among these activities, the creation of a marine forest using artificial reefs requires a huge budget for the construction and dropping of structures, and transplantation and management of seaweeds. In the case of the imitation pouch facility, there is a risk of being eaten by various early animals in the early stages of spore germination, and washing the seafloor. In the case of work, there is a limitation that it is limited to shallow coasts due to the nature of the work.

Recently, a method of creating a marine forest using seaweed transplantation in which seaweed is fixed to an artificial substrate or natural rock is widely used for the creation of a marine forest. After culturing the seaweed to be transplanted to induce epiphysis on a transplant rope, various methods are applied to the artificial substrate or natural rock. There is a method of fixing the seaweed by the method of fixing it and the method of collecting seaweeds living in the vicinity and fixing the seaweeds with a zip tie directly to the transplantation rope fixed to an artificial substrate in advance.

This type of transplantation method can be installed only on artificial substrates, and it is difficult to apply to natural rock with an irregular surface. In addition, the implant target species can be applied as long as the bulk growth techniques have been established with a stable or Ecklonia cava (Ecklonica cava) to form a large dendritic gageun, gompi (Ecklonia stolonifera), kelp (Saccharina japonica) and Sargassum (Sargassum fulvellum) Algae . On the other hand, perennial mothers and daughters that form patchy or conical rhizoids , such as Sargassum patens , Sargassum serratifolium, Sargassum macrocarpum, and Sargassum siliquastrum, are inoculated into oviposites. It is difficult to do, and mass culture research is still in its infancy, and direct transplantation methods have not been studied.

Recently, there has been proposed a method of using embryos or young cultures of large-fruited mother-of-pearl and mother-of-pearl on artificial substrates or natural stones for use in the composition of a marine forest. However, in order to apply this method to marine forests, high material, human, and time resources such as materials, equipment and culture facilities, management, etc. are required in the process of collecting mature reproductive deposits, embryo harvesting, embryo inoculation and germination, indoor cultivation and field transplantation. In addition, if a problem occurs in each step, there is a problem that further steps cannot be carried out.

In addition, young fronds that are not sufficiently developed become targets of various brood animals, so additional time and human and material resources are required to install a brood animal prevention net after field transplantation.

A method of transplanting seaweed seedlings by attaching cultured seaweed filament slices using epoxy resin and Velcro tape to create a marine forest (Korean Patent Registration No. 10-1830034), from cement, sand, retarder and stone powder as main components A method of applying an adhesive and attaching seaweeds and corals to install a pressing assembly so that an implant can be seated on an underwater structure (Korean Patent No. 10-1069721), etc., are disclosed, but the inventions include Ecklonia, kelp, It can be applied only to seaweeds that form large rhizoids (or holdfasts) such as gomphyll, but it is not applicable to many perennial Sargassaceae seaweeds with discoid or conical rhizoids, Attachment and removal of Velcro taping to bond seaweed cultured workers to bedrock, assembly and installation work to maintain the adhesion of seaweed implants using cement-based adhesives, production of specially designed plastic implants, and complex seaweed attachment and underwater drilling There is a problem in that human, material, and time resources are greatly consumed due to complicated equipment and processes such as seaweed transplantation through work and long underwater work.

Republic of Korea Patent No. 10-1830034 Republic of Korea Patent Registration No. 10-1069721

(Public Work Order 001) Nara Market Bidding Notice 20200512254-00

An object of the present invention is to provide a structure for transplanting seaweeds in which an attachment group (rhizome ) of perennial Sargassaceae seaweeds with a frond length of 10 to 30 cm is fixed to the surface of an attachment substrate with an adhesive, a manufacturing method thereof, and a marine forest composition or restoration using the structure to provide a way

In order to achieve the above object, the present invention provides a structure for transplanting seaweeds in which the attachment group ( rhizome ) of perennial Sargassaceae seaweeds having a frond length of 10 to 30 cm is fixed to the surface of the attachment substrate with an adhesive.

The adhesion substrate may be made of one selected from the group consisting of a cured epoxy resin, stone, ceramic, plastic, and concrete.

The attachment substrate may be in the form of a button having one or more holes formed therein.

The attachment substrate may be in the form of a button having one or more holes formed of a cured epoxy resin.

The button shape may be a polygonal or circular shape having a width or diameter of less than 3 cm to 6 cm, and a thickness of 0.5 to 1.5 cm.

The attachment substrate may further include a screw and a Carl Plug inserted into the hole.

In addition, the present invention provides a step of preparing perennial Sargassaceae seaweed and an attachment substrate having a frond length of 10 to 30 cm, and attaching the perennial Sargassaceae seaweed attachment group (rhizome) to the surface of the attachment substrate as an instant adhesive Provided is a method of manufacturing a structure for transplanting seaweed comprising the step of fixing.

In the step of preparing the attachment substrate, a semi-solid epoxy resin and a curing agent are mixed and the width or diameter is 3 cm to 6 cm, and after molding into a polygonal or circular button shape with a thickness of 0.8 to 1.2 cm, a hole in one corner It may be to penetrate and harden.

In addition, the present invention comprises the steps of (a) determining an adherent substrate and transplanted seaweed suitable for the environment of the area to be created or restored in the marine forest, (b) manufacturing or preparing the adherent substrate determined in step (a), (c) the above (b) fixing the attachment group (rhizome) of the seaweed to the attachment substrate produced or prepared in step (b) with an adhesive to prepare a structure for transplanting seaweed, and (d) dropping or fixing the structure for transplantation of seaweed into the target area It provides a method of creating or restoring a marine forest comprising a.

Algae graft structure of the present invention was prepared by attaching with an adhesive agent to the epoxy resin, a natural stone (rock, stone), ceramic, plastic or concrete panel curing the perennial Sargassum in (Sargassum) are known to be hard-to-magnetic implant, the structure By installing it in the water, it is possible to transplant maternal and semi-adults with a simple process and low cost. Therefore, the structure can be usefully utilized for the creation or restoration of a marine forest, and it can be used as a component of a seawater aquarium, which has recently been expanded in the market.

In addition, although the number of transplanted individuals may be small compared to the composition by culture, the composition of the marine forest through adult mother and child transplantation may prevent the leaflets from falling off the substrate and damage due to physical shocks such as waves, and damage from eating by brood animals. , it is economical in terms of material, human, and time resources as it can survive for many years through a single transplant and serve as a spore sac that produces and releases spores every year.

1 shows the process of manufacturing and transplanting a structure for transplanting individual seaweeds using Ecklonia cava or Sargassum macrocarpum and an epoxy resin that has been cured and molded into a button shape.
Figure 2 is a button-shaped molded into a hardened epoxy resin and attached to the natural bedrock individually transplanted Ecklonia cava (top) and large fruit cap ( Sargassum macrocarpum , bottom) 6 months after transplantation of the fronds and attachment phase growth patterns it has been shown
Figure 3 shows the survival rate, survival rate, leaf length, and growth rate (change in length of leaf length) of Ecklonia cava and Sargassum macrocarpum , which were individually transplanted into natural bedrock by molding into a button shape and attaching to a cured epoxy resin. %) is a graph as a result of follow-up for 6 months.
4 is a twist Sargassum (Sargassum siliquasturm) structure (seokbujak) production process for transplantation using a concrete panel with a natural stone (① to ⑧) and the initial water installation state (⑨: concrete panel, ⑪: Natural Stone) and algae growth conditions after 3 months (⑩: concrete panel, ⑫: natural stone) is shown.
The white color in ① of FIG. 4 is the point 400 for attaching the seaweed attachment group (rhizome) through the epoxy resin, and the red dotted circle is the point 500 for attaching the seaweed attachment group (rhizome) directly to the attachment substrate.
5 shows the manufacturing process and installation (① to ⑦) of a structure for transplantation of Sargassum fulvellum using stones (Sargassum fulvellum), the initial underwater installation state (⑧), and the seaweed growth state (⑨) after 3 months.
6 shows that as a result of transplantation of large berry caps using the epoxy resin and wire of the comparative example (bid notice No. 20200512254-00), the drop-off of the epoxy resin (③) and the detachment of the leaflet due to necrosis of the attachment phase (④) occurred frequently.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein and the experimental methods described below are those well known and commonly used in the art.

In order to achieve the above object, the present inventors have a pseudodiscoid attachment group with an adhesive to a seaweed attachment structure containing a cured epoxy resin, natural stones (stones, rocks) of various sizes, and concrete panels ( Sargassum fulvellum ) and Sargassum siliquasturm having a discoid attachment group was attached, and the seaweed transplantation of the present invention was monitored by transplanting and dropping it at a depth of 3 to 5 m of the coast exposed to the topography and moderate waves concealed by the waves. The present invention was completed by confirming that the survival rate of seaweed transplanted with the dragon structure was excellent.

In one aspect, the present invention relates to a structure for transplanting seaweeds in which an attachment group ( rhizome ) of perennial Sargassaceae seaweeds having a frond length of 10 to 30 cm is fixed to the surface of an attachment substrate with an adhesive.

The structure for transplanting seaweed of the present invention is characterized in that the attachment group ( rhizome) of perennial Sargassaceae seaweeds with a frond length of 10 to 30 cm is attached and fixed to the surface of the attachment substrate with an adhesive.

In the present invention, the attachment device (rhizome) serves to fix the body of the seaweed to a hard object such as a rock or pile, and is divided into 4 types: fibrous, pseudodiscoid, discoid, and conical depending on the shape. classified into two types.

The perennial Sargassaceae seaweed of the present invention is characterized in that it is an adult with a frond length of 10 to 30 cm.

In the present invention, when the frond length is less than 10 cm, growth failure may occur due to competition for light and nutrients with the surrounding seaweed, and if it exceeds 30 cm, the dropout rate may increase due to physical factors such as waves after transplantation.

The perennial Sargassaceae seaweeds are bisexual capsid ( Sargassum patens ), Endo mojaban ( Sargassum yendoi ), sawtooth cap ( Sargassum serratifolium ), Myapsissum confusum ( Myapsissum confusum ), solitary capsicum Seaweed consisting of a discoid attachment group, including Sargassum siliquastrum and Sargassum muticum; Seaweed consisting of a conical attachment group, including Sargassum macrocarpum , Sargassum micracanthum and Sargassum coreanum; Seaweed consisting of the pseudodiscoid attachment group, including Sargassum fulvellum; and Sargassum fusiforme and Sargassum hemiphyllum may be at least one selected from the group consisting of fibrous attachment groups including seaweeds.

In the present invention, the structure for transplanting seaweed is characterized in that one or more adults of Sargassaceae seaweed are attached. Specifically, when two or more seaweeds are fixed to the attachment substrate, each seaweed may be fixed or attached at intervals of 10 to 20 cm.

In the present invention, when the distance between seaweeds is less than 10cm, growth problems may occur in some individuals due to competition in light and nutrients during growth of seaweeds, and growth failure may occur in some individuals. And there is a problem in that the physical defense effect due to the wave is reduced.

The structure for transplanting seaweed according to the present invention may be one in which lobed perennial macro-brown algae are additionally fixed to the surface of the attachment substrate to which the mother and algae are attached.

The lobed perennial large brown algae may be lobed brown algae having a dendritic attachment period ( rhizome), specifically Ecklonica cava , rhubarb ( Eisenia bicyclis ), Ecklonia stolonifera , selected from the group consisting of kelp ( Saccharina japonica). There may be more than one type.

In addition, the structure for transplantation of seaweed, in which the perennial mother and mother and algae and lobular brown algae having a dendritic attachment period (rhizome) are mixed, may be more advantageous in increasing the survival rate after transplantation of seaweed than a structure for transplantation made of a single species of seaweed. It can be usefully utilized to create this improved marine forest.

In the present invention, the attachment group is fixed to the surface of the attachment substrate using an adhesive.

In the present invention, the adhesive may be a cyanoacrylate-based or ethylcyanoacrylate-based adhesive as an instant adhesive.

Specifically, if the seaweed to be attached is conical or dendritic seaweed, an ethyl cyanoacrylate-based adhesive, which is inexpensive in terms of cost, can be used. It is preferable to use an acrylic acid-based adhesive.

In an embodiment of the present invention, in order to solve the difficult problem of perennial mother and child genus (Sargassum ) adult transplantation of seaweed having a narrow patchy or removable attachment (rhizome), Sargassum macrocarpum , Sargassum fulvellum ) ( Sargassum siliquastrum ) flatly trimmed and adhered to the attachment substrate with an instant adhesive to prepare a structure for transplantation of seaweed, and after fixing or dropping it in water, the survival rate of seaweed was monitored for 3 months. Cases showed a high survival rate of 70% or more, and in particular, when transplanted to natural rock exposed to waves as an example, 6 months after transplantation, Ecklonia cava has a high survival rate of about 67%, and perennial mother and child birthmarks have a normal growth pattern with a high survival rate of 71%. showed

In addition, as a result of comparing the method in which the grafted seaweed was directly fixed to the adherent substrate with an instant adhesive in the embodiment of the present invention and the method in which the grafted seaweed was fixed thereon after attaching and curing an epoxy resin to the adherent substrate in advance, the survival rate between the two test groups was It was confirmed that there was no significant difference. Therefore, it is preferable to directly attach the attachment group of the transplanted seaweed with the instant adhesive to the attachment substrate in order to simplify the process and reduce material cost.

In addition, in an embodiment of the present invention, in order to compare the survival rate after transplantation of seaweed according to types of attachment substrates, a structure for transplanting seaweeds was prepared by fixing Sargassum siliquastrum to a concrete panel or surface of a natural stone, and after transplanting in water , As a result of analyzing the survival rate of seaweed, it was confirmed that there was no significant difference in the survival rate between the two test groups of concrete panel and natural stone.

In addition, as a comparison of the present invention, as a result of observing the growth of a structure for implantation bonded with an epoxy resin under water after transplantation, it is dropped before the epoxy resin is completely cured, and the entire leaflet together with the epoxy resin is dropped, or an epoxy resin-coated attachment device It was confirmed that the part was necrotic due to lack of photosynthesis and cellular respiration, and the growth was not maintained 2 months after transplantation due to the falling off of the fronds.

The adhesion substrate of the present invention is not easily corroded in water, is formed of a solid material, and may be made of one selected from the group consisting of specifically hardened epoxy resin, stone, ceramic, plastic and concrete.

The adhesion substrate of the present invention depends on the environment (low-layer substrate composition such as bedrock, gravel, sandy and artificial structures, distance from the coast, habitat condition of birds of prey, depth of water, topography and physical disturbance, degree of waves) on the target site for seaweed transplantation. Various sizes and weights may be formed.

Specifically, in a bay terrain with very little physical disturbance such as waves, it is effective in terms of work efficiency such as transportation to select an attachment substrate of a stone with a relatively small size and weight, and select a rock with a diameter of 50 cm or more in a terrain exposed to moderate waves. This is advantageous in terms of stability of the graft substrate, and when the bottom layer of the transplant site is composed of rock and there is difficulty in transporting the adherent substrate because it is far from the coast, it is used for transplantation of seaweed manufactured with an adherent substrate in the form of a button with a hole formed therein. structures are available. The attachment substrate in the form of a button having the hole may be fixed to the natural rock by inserting a screw into the hole and using the screw. In addition, concrete panels can be selected for application to artificial reefs.

In an embodiment of the present invention, it was confirmed that Ecklonia cava and Sargassum macrocarpum mainly inhabit in bedrock with a depth of 5 to 15 m, where they are exposed to moderate or higher waves and have good tidal communication, and are exposed to the open sea. It was judged that it was more effective in terms of cost and manpower to transplant individual seaweeds by using small and light attachment substrates rather than heavy substrates such as bedrock or panels for transplantation into flat rocky terrain. Therefore, after molding into a square or round button shape with a diameter of about 5 cm and a thickness of about 1 cm, drill a hole in one corner of the same diameter as a screw and harden it to manufacture a button-shaped attachment substrate, and Ecklonia cava ( Sargassum macrocarpum ) After removing moisture from the attachment group, it was fixed to the button-type attachment substrate using an instant adhesive. After inserting a screw into the hole of the prepared Ecklonia cava or Sargassum macrocarpum implantation structure and drilling it in the underwater rock at a depth of about 5 m, inserting a knife block into the perforation, Ecklonia cava ( Sargassum macrocarpum ) Ecklonia cava ) or Sargassum macrocarpum ) The screws of the structure for transplantation were fixed to the bedrock using a hammer. The number of transplanted seaweeds is 61 specimens, 33 Ecklonia and 28 Sargassum macrocarpum.

As a result of monitoring the transplanted seaweed, after 6 months, 22 specimens survived, showing a survival rate of 66.7% for Ecklonia cava, and 20 individuals for H. fertii, which recorded a survival rate of 71.4%. Overall, the survival rate was 68.9%.

As a result of the growth analysis, the average leaf length of E. Ecklonia was 23.1 cm at the time of transplantation, but the leaf length showed a tendency to slightly decrease to 15.6 cm one month after transplantation. seemed In the case of large berry caps, the growth rate was low at 12.9 cm at the time of transplantation, less than 1 cm for 2 months, and then showed a rapid growth of about 5 cm from March, 3 months after transplantation.

In other words, it was confirmed that seaweed transplantation using the structure according to the present invention was effective by maintaining a survival rate of 66.7% to 71.4% for 6 months after transplantation of seaweed (L. .

Therefore, the attachment substrate of the present invention may be in the form of a button having one or more holes, specifically, it may be in the form of a button having one or more holes formed of a cured epoxy resin.

In addition, the button-type attachment substrate of the present invention may further include a screw and a Carl Plug inserted into the hole.

The hole may be formed to have the same size as the diameter of the screw.

The button shape may be a polygonal or circular shape having a width or diameter of less than 3 cm to 6 cm, and may be formed to a thickness of 0.8 to 1.2 cm.

The attachment period of the perennial Sargassaseae extends up to 3cm in diameter at the attachment site, and in perennial lobed seaweeds with dendritic rhizoids, the rhizoids extend to the low bedrock and fix it 2 months after transplantation, so the width or diameter of the button-shaped attachment substrate It is pointless to extend beyond this 6 cm.

If the thickness of the button-type attachment substrate is less than 0.8 cm, there is a risk of damage to the graft substrate when it is fixed to the bedrock using screws. If the thickness of the button-shaped attachment matrix is more than 1.2 cm, the transplantation efficiency may be low because it takes time for the attachment mechanism of large brown algae with dendritic rhizoids to expand into the natural bedrock.

In addition, in another embodiment of the present invention, a structure for transplanting seaweeds was prepared by fixing Sargassum siliquastrum on the surface of a natural stone having a diameter of about 50 cm and a weight of 10 to 30 kg with an instant adhesive. It was confirmed that the survival rate of Sargassum siliquastrum for months was as high as 80% or more.

Therefore, the adhesion substrate of the present invention may be made of stone.

In the present invention, the stones are made of igneous rocks, sedimentary rocks, and rocks including metamorphic rocks, and are called natural stones, rocks, and stones (pebbles).

Specifically, it may be a natural stone of 10 to 30 kg or a natural stone having a diameter or width of 40 to 60 cm.

In addition, in another embodiment of the present invention, a structure for transplanting seaweed was prepared by fixing Sargassum siliquastrum on the surface of a 25cm x 40cm x 5cm concrete panel with an instant adhesive. It was confirmed that the survival rate of Sargassum siliquastrum ) was as high as 80% or more. Therefore, the concrete of the present invention has an area of 20 to 30 cm x 35 to 45 cm and may be a concrete panel having a thickness of 2.5 to 7.5 cm, specifically, a concrete panel having an area of 25 cm x 40 cm and a thickness of 5 cm.

In addition, the final compound of the present invention a further embodiment the diameter 20cm and out, the weight 1 to 3kg for algae implant structure the staff purpura (Sargassum fulvellum) the stone surface is fixed to the instant adhesive in the, their transplantation results staff purpura (Sargassum fulvellum ) was confirmed to maintain a high survival rate of 78.3%.

Therefore, the adhesion substrate of the present invention is a stone, and specifically, it may be a stone of 1 to 3 kg or a stone of 15 to 25 cm in diameter.

In addition, the present invention provides a step of preparing perennial Sargassaceae seaweed and an attachment substrate having a frond length of 10 to 30 cm, and attaching the perennial Sargassaceae seaweed attachment group (rhizome) to the surface of the attachment substrate as an instant adhesive It relates to a method of manufacturing a structure for transplanting seaweed comprising the step of fixing.

In the present invention, the description of "perennial Sargassaceae seaweed" and "adherent substrate" is the same as described above.

The step of preparing the adhesion substrate of the present invention may be preparing a substrate made of one selected from the group consisting of a cured epoxy resin, stone, ceramic, plastic and concrete.

When the adhesion substrate is made of a cured epoxy resin, the step of preparing the adhesion substrate of the present invention is 3 cm to 6 cm in width or diameter by mixing a semi-solid epoxy resin and a curing agent, and a polygonal thickness of 0.8 to 1.2 cm Alternatively, it may be molded into a circular button shape and then hardened by punching a hole in one corner.

In the present invention, the step of preparing the seaweed may include a process of flattening the attachment device and removing moisture before bonding. Specifically, by trimming the attaching machine to widen the bonding area, it is possible to make it well fixed by the adhesive.

In addition, the attachment strength may be increased by removing foreign substances from the seaweed attachment device (rhizome).

In addition, it may be to remove the moisture of the algae attachment device in the capsular family to increase the fixing force by the adhesive. If the moisture of the attachment device is not completely removed, the adhesive force may decrease and the algae may not be fixed to the support object.

In an embodiment of the present invention, the pretzel cap ( Sargassum siliquastrum ) was treated with air at 4 to 20 ° C. for 10 to 60 seconds using an air gun to remove moisture. For moisture removal, the treatment time was different depending on the ambient temperature, but the treatment was usually performed within 60 seconds.

Therefore, the present invention may be to remove moisture by treating the attachment device with an air gun using air between 2° C. and 30° C. for moisture removal.

When the temperature of the air is less than 2 degrees, the adherent phase is frozen, or when it exceeds 30°C, it may cause damage to the adherent cell tissue due to heat.

In another embodiment of the present invention, after removing the foreign substances of the staff jaban attachment device, moisture was removed from the attachment site using a moisture absorbent paper.

Therefore, the removal of the moisture may be to remove the moisture of the attaching machine (rhizome) with a material capable of absorbing moisture including paper or cloth, and specifically, it may be to remove the moisture using a moisture absorbent paper.

In the present invention, the fixing step is to apply an adhesive to the surface of the attachment group (rhizome ) or attachment substrate of perennial Sargassaceae seaweed, and to attach and fix the seaweed attachment group and attachment substrate surface.

Description of the "adhesive" in the present invention is the same as described above.

In addition, the present invention comprises the steps of (a) determining an adherent substrate and transplanted seaweed suitable for the environment of the area to be created or restored in the marine forest, (b) manufacturing or preparing the adherent substrate determined in step (a), (c) the above (b) fixing the attachment group (rhizome) of the seaweed to the attachment substrate produced or prepared in step (b) with an adhesive to prepare a structure for transplanting seaweed and (d) applying the structure for transplantation of seaweed prepared in step (c) to the target area It relates to a method of creating or restoring a marine forest comprising the step of dropping or fixing to the .

In the present invention, "environment" refers to the composition of low-layer substrates such as bedrock, gravel, sandy and artificial structures, distance from the coast, habitat status of birds of prey, water depth, topography and physical disturbance, Indicates the degree of wave.

In the present invention, the step of determining the adherent substrate and transplanted seaweed in step (a) includes the composition of the low-layer substrate such as bedrock, gravel, sandy and artificial structures in the area to be created or restored in the sea forest, the distance from the coast, the habitat condition of the brood animals, It means observing or predicting the depth of water, topography and physical disturbance, and the degree of waves, and selecting the type of seaweed and attachment substrate suitable for the environment.

Specifically, in the step of determining the adhesion substrate, it is effective in terms of work efficiency, such as transportation, to select an adhesion substrate of a stone having a relatively small size and weight in a bay terrain with very little physical disturbance such as waves, and a terrain exposed to moderate waves In Korea, it is advantageous in terms of stability of the transplantation substrate to select a rock with a diameter of 50 cm or more, and if there is difficulty in transporting the attachment substrate because the bottom layer of the transplant site is composed of bedrock and it is far from the coast, it can be fixed to the bedrock using screws. You can choose a button-type hardened epoxy resin, and to apply it to an artificial reef, you can choose a concrete panel.

The step of determining the seaweed is to determine the transplanted seaweed species suitable for the environment, and specifically, in places where the number of brood animals such as sea urchins is large, Sargassum fulvellum , Ecklonia cava , and kelp ( Saccharina japonica ). It may be to exclude seaweed species with high damage and to select seaweed species that are resistant to corrosion, such as Sargassum macrocarpum and Sargassum siliquastrum, selected Ecklonia cava (Ecklonia cava) and large berries Sargassum (Sargassum macrocarpum) mainly live in, and that in the shallow areas of the shipbuilding nearby pretzel Sargassum (Sargassum siliquastrum) and jjakip Sargassum (Sargassum hemiphyllum) and live in shallow water in the natural state, as It may be to select a species of seaweed.

The transplanted seaweed of the present invention may be selected as a single species suitable for the environment of the marine forest formation area or by mixing 2 to 3 types of seaweed according to the purpose.

The transplanted seaweed of the present invention may be at least one selected from perennial Sargassaceae seaweed and lobed perennial macro-brown algae.

In the present invention, the description of “perennial Sargassaceae seaweed” and “flaky perennial macro-brown algae” is the same as described above.

It is known that most of the leaves and fronds of perennial mother and algae are thick leathery or cartilaginous when they are adults, and have allelopathic substances in the fronds that can prevent damage to breakfast. Therefore, when the transplanted seaweed is a perennial motherwort family, it is possible to prevent the fronds from falling off and damage from the substrate due to physical impacts such as waves, and damage from eating by incubating animals.

In another embodiment of the present invention, a seaweed transplantation structure based on a button-type attachment matrix that is smaller and lighter than transplanting seaweeds by transporting a heavy attachment matrix to a bedrock located at a relatively long distance from the coast and exposed to waves at a depth of about 5 m. It was judged to be more effective in terms of cost and manpower, therefore, after molding and curing an epoxy resin into a button shape with a diameter of about 5cm and a thickness of about 1cm, Sargassum macrocarpum and Ecklonia cava were instantaneously applied. When the method of attaching it with adhesive and fixing it to the natural bedrock with screws and knife blocks was used, it was confirmed that about 67% of Ecklonia cava and about 71% of Ecklonia vulgaris were alive without falling off from the substrate.

Therefore, in the natural bedrock of the terrain where the seaweed transplantation target area is far from the coast or exposed to waves, a hole is formed in a polygonal or circular shape with a width or diameter of less than 3 cm to 6 cm, and a button-shaped hardening with a thickness of 0.8 to 1.2 cm. Epoxy resin can be selected as an adhesion substrate, and seaweed can be selected from Sargassum macrocarpum or Ecklonia cava .

In another embodiment of the present invention, Sargassum siliquastrum was observed to be a colony in the bedrock near the low sea level of the coast where moderate waves hit, and it was confirmed that the growth environment of the Twitches Hat was the condition of moderate waves, and thus, the Twitches hat was found. ( Sargassum siliquastrum ) A structure for transplantation of attached seaweed was transplanted to an area with moderate waves. At this time, in order to minimize the risk of overturning or loss of the transplant structure due to waves, an attachment substrate having a weight of 10 to 30Kg was used. As a result of the survival rate analysis of the transplanted twist hat spots, it was confirmed that they survived without losing more than 80%.

Therefore, in the terrain exposed to moderate waves of the present invention, it is possible to select an adhesion substrate of 10 to 30Kg.

If an adhesion substrate of less than 10Kg is used in an area exposed to moderate waves, the structure for transplantation may be lost or overturned due to the physical disturbance of the waves after transplantation. have.

In addition, it was confirmed that the growth environment of the Twitch cap is a condition of moderate waves, and in the case of an area exposed to moderate waves, Sargassum siliquastrum can be selected as a seaweed of the Sargassaceae family.

In another embodiment of the present invention, Sargassum fulvellum colonies were observed in shallow water depths of bay terrain concealed from waves, and a structure for transplanting seaweeds was prepared by using 1 to 3 kg of stones with a diameter of about 20 cm as an attachment substrate, transplanted. As a result of analyzing the survival rate of the transplanted staff purpura, it was confirmed that they survived without losing more than 78%.

Therefore, in the case of a bay terrain with a shallow depth of 2 to 3 m, where the area to be created or restored is hidden from waves, stones with a diameter of 15 to 25 cm in diameter may be selected as an attachment substrate to increase work efficiency.

In addition, an attachment substrate having a weight of 1 to 3 kg can be selected.

In the above, Sargassum fulvellum is observed in the shallow water depth of the bay topography concealed from the waves. You can choose.

In the present invention, step (b) is a step of preparing or preparing the adhesion substrate determined in step (a).

In the step of preparing the adhesion substrate of the present invention, a substrate made of one selected from the group consisting of a cured epoxy resin, stone, ceramic, plastic and concrete may be prepared.

When the adhesion substrate is made of a cured epoxy resin, the step of preparing the adhesion substrate of the present invention is 3 cm to 6 cm in width or diameter by mixing a semi-solid epoxy resin and a curing agent, and a polygonal thickness of 0.8 to 1.2 cm Alternatively, it may be molded into a circular button shape and then hardened by punching a hole in one corner.

In the present invention, step (c) is a step of manufacturing a structure for transplanting seaweed by fixing the attachment group (rhizome) of the seaweed to the prepared or prepared attachment substrate with an adhesive.

The manufacturing step of the present invention is to apply an adhesive to the surface of the attachment group (rhizome ) or attachment substrate of perennial Sargassaceae seaweed, and attach and fix the seaweed attachment group and attachment substrate surface.

In the present invention, the description of "perennial Sargassaceae seaweed" and "adhesive" is the same as described above.

The manufacturing step of the present invention may include a process of flattening the seaweed attachment device and removing moisture before fixing the attachment device. Specifically, by trimming the attaching machine to widen the bonding area, it is possible to make it well fixed by the adhesive.

Step (d) of the present invention is a step of dropping or fixing the structure for transplanting seaweed prepared in step (c) to the target area.

In the present invention, dropping refers to the operation of moving the structure for transplantation of seaweed to the desired location for transplantation. Specifically, it means dropping and locating a seaweed transplantation structure including a concrete panel and an adherent substrate of stones (natural bedrock, stone, etc.) on a target area for seaweed transplantation.

In the present invention, fixing may be fixing using screws so that the structure for transplanting seaweed is not separated from the target area. Specifically, after drilling the natural rock with a drill, inserting a knife block and fixing the structure by connecting a screw with a structure for transplanting seaweed including a button-shaped attachment substrate with a hole formed in the knife block.

In the present invention, fixing may be fixing one seaweed by attaching one seaweed to a button-shaped attachment substrate having a perforation for fixing screws, and drilling holes in the bedrock at intervals of 10 to 20 cm using screws and knife blocks.

In addition, Sarrassaceae seaweed and foliar perennial macro-brown algae are crossed at a ratio of 1:1 and fixed to natural bedrock at intervals of 10 to 20 cm.

Specifically, Sargassaceae seaweed or lobed perennial large brown algae were attached to a button-type attachment substrate with a screw-fixing hole with an instant adhesive, and Sargassaceae seaweed and lobed perennial large brown algae were attached to the natural bedrock. may be crossed at a ratio of 1:1 and fixed with screws, and more specifically, crossed with Sargassaceae seaweed and lobed perennial macro-brown algae at a ratio of 1:1 and placed on natural bedrock at intervals of 10 to 20 cm. It may be fixed using screws.

In the present invention, dropping or fixing the structure for transplantation is preferably carried out before the start of rapid growth of the transplant target seaweeds and the effect of typhoons is less in consideration of the restoration of the marine forest or the physical disturbance of the composition site. If the transplant target seaweed is transplanted during a period of rapid growth, the rate of leaf fall due to physical disturbance may increase because the growth rate of the transplanted seaweed is faster than the rate at which the attachment period of the transplanted seaweed is sufficiently settled on the substrate.

Sargassum fulvellum (Sargassum fulvellum) forms countless side branches in the coast of Jeju Island in January to April, grows explosively, forms a reproductive deposit in March to April, rapidly declines after sexual reproduction, and forms new shoots near the base. Grows slowly until November, and the large berry cap ( Sargassum macrocarpum ) and the twist cap ( Sargassum siliquastrum ) grow rapidly from January, when the water temperature is the lowest, and form genital deposits in May to July and the rest except for the central branch after sexual reproduction. After the leaflet is removed, the remaining primary branches grow slowly from August to December, and Ecklonia cava grows slowly in November to January and then rapidly grows in February to June 7 From September to September, a genital deposit is formed and spores are released, and end-greening occurs in October.

In addition, in Korea, it is desirable to transplant seaweeds during this period, since June to September is a time when typhoons are frequently attacked and at the same time , the feeding activity of broccoli fish such as Siganus fuscescens and Girella punctata is active. don't

Therefore, it was judged that October to January is the most suitable transplantation period for Sargassum macrocarpum , Sargassum siliquastrum and Sargassum fulvellum. , the staff member was transplanted in January.

Therefore, in the present invention, when the seaweed attached to the structure for transplanting seaweed is Sargassum macrocarpum , it can be transplanted in December, and in the case of Sargassum siliquastrum , it can be transplanted in October, and the If the seaweed attached to the structure for transplanting seaweed is Sargassum fulvellum , it may be transplanted in January.

Hereinafter, the configuration and effects of the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.

1. Collecting transplanted seaweed

Sargassum siliquastrum (Sargassum siliquastrum) is at a depth of 1 to 2 m off the coast of Sinyang-ri, Seongsan-eup, Jeju-do, and Sargassum fulvellum is a seaweed leaf attachment (rhizome) with a central branch frond length of 10-30 cm at a depth of 2 m off the coast of Hagwi 1-ri, Aewol-eup, Jeju-si ) was completely removed from the substrate and collected.

2. Selection of seaweed transplantation time

Waves generated by typhoons can cause destructive physical disturbance to the seaweed community inhabiting the coast, and in Korea usually have direct or indirect effects from June to September. The shallow area within 5m of the subtidal zone is one of the biggest factors hindering the dropout rate or survival rate of transplanted seaweeds because physical disturbance by waves is more frequent than the deep water zone. Moreover, June to September, when the water temperature is relatively high, is a period when the feeding activity of broccoli fish such as Siganus fuscescens and Girella punctata is active, and feeding damage by the transplanted seaweed fish frequently occurs.

In addition, many seaweed species have a period of relatively slow growth and a period of rapid growth and explosive growth of 2 to 3 times the length of the existing fronds in about one month. This difference in the growth rate of seaweed is known to be caused by changes in water temperature, and the period of rapid growth is different for each seaweed species.

It is desirable to transplant seaweeds before the start of rapid growth of the transplanted seaweeds and the effect of typhoons is small in consideration of the restoration of the sea forest or the physical disturbance of the site. If the transplant target seaweed is transplanted during the rapid growth period, if the growth rate of the transplanted seaweed is faster than the rate at which the attachment period of the transplanted seaweed is sufficiently settled on the substrate, the rate of leaf loss due to physical disturbance may increase.

Ecklonia cava grows slowly in November to January, then grows rapidly in February to June, forms genital deposits in July to September, releases spores, and the end rust phenomenon occurs in October.

Sargassum macrocarpum and Sargassum siliquastrum grow rapidly from January, when the water temperature is the lowest, and form genital deposits in May to July and after sexual reproduction, the rest of the fronds except the central branch fall off until December. The remaining primary branches show a slow-growing pattern.

Therefore, in consideration of these growing seasons, Ecklonia and large berry caps were transplanted in December, and in October for twisted caps.

Sargassum fulvellum (Sargassum fulvellum) forms countless side branches in the coast of Jeju Island in January to April, grows explosively, forms a reproductive deposit in March to April, rapidly declines after sexual reproduction, and forms new shoots near the base. It grows slowly until November.

Considering this growth period, although October to December is the ideal time for transplantation, it was inevitably transplanted in January due to the experimental schedule.

3. Selection of transplant depth

For natural algae communities of the island coast Ecklonia cava (Ecklonia cava) has a depth of 5 to 20m, a large fruit Sargassum (Sargassum macrocarpum) has a depth of 5 to 15m, pretzel Sargassum (Sargassum siliquastrum) and staff purpura (Sargassum fulvellum) is primarily tidal flat bottom to Since it was observed well in the subtidal range of 5 m, the structure for transplantation of seaweed was transplanted into the subtidal zone for Ecklonia genitalia and H. oleracea within 5 m, and for Quercus serrata and chamomile purpura in the subtidal zone at a depth of 2 to 3 m.

4. Selection of transplant site

In the case of natural seaweed colonies off the coast of Jeju Island, in the case of Ecklonia cava and Sargassum macrocarpum , exposure to moderate or higher waves and good flow of algae in bedrock with a depth of 5 to 15 m, Sargassum siliquastrum is moderate. It is mainly observed in the rock formations near the low-water mark of the coast where waves hit, and Sargassum fulvellum was observed in shallow waters in the bay topography concealed from the waves. In consideration of these topographical and physical characteristics, Ecklonia cava and Sargassum macrocarpum are made of flat bedrock with a depth of about 5m exposed to the open sea in Jongdal-ri, Gujwa-eup, Jeju-do, and Sargassum siliquastrum is a wave of Pyeongdae-ri, Gujwa-eup, Jeju-do. A rocky area with a depth of about 2 m in the slightly affected area was selected for transplantation, and for Sargassum fulvellum , a sandy area with a water depth of about 3 m in Sasupo-gu, Sasu-dong, Jeju-si, where there is little effect of waves, was selected as a transplant site.

5. Manufacture of structures for transplantation of seaweed by attachment substrate

Example 1) Preparation of a structure for implantation based on a button-type attachment substrate

In order to transplant into the flat rock topography exposed to the open sea, it was judged that it was more effective in terms of cost and manpower to transplant each seaweed by using a small and light attachment substrate rather than a heavy substrate such as bedrock or panel.

Therefore, in order to transplant Ecklonia cava and Sargassum macrocarpum on flat rock formations exposed to the open sea, a button 100 formed of an epoxy resin cured as an adhesion substrate was selected as a substrate. Epoxy resin has advantages in that it is easy to manufacture in a button shape due to its excellent moldability, is hard material after hardening, and remains in its first molded form without being decomposed or deformed for many years even in water. The button shape is formed with a hole 200 for inserting the screw. Using the screw inserted into the hole, Ecklonia cava or large berry cap can be fixed to the natural bedrock.

In order to transplant each seaweed into natural rock, a structure for transplanting seaweed was prepared as follows using a button-type attachment substrate.

As the adherent seaweed, Ecklonia cava and Sargassum macrocarpum were used.

① After mixing semi-solid epoxy resin and curing agent, it was molded into a square or round button shape with a diameter of about 5 cm and a thickness of about 1 cm, drilled a hole in one corner of the same diameter as a screw, and dried naturally for 24 hours to harden (Fig. 1-①).

② After removing foreign substances from the large berry cap and Ecklonia cava attachment machine, smooth the attachment area with a sharp knife (Figs. 1-②, ③), and use an air gun on the attachment area for 10 to 60 seconds with air at 4 to 20°C. treatment to remove moisture (FIGS. 1-④). For moisture removal, the treatment time was different depending on the ambient temperature, but the treatment was usually performed within 60 seconds.

③ The instant adhesive was applied (Fig. 1-⑤) and attached to the attachment substrate (Fig. 1-⑥), and screws were inserted into the screw holes of the attachment substrate (Fig. 1-⑦, ⑧). ).

④ After drilling using a drill in the underwater rock at a depth of about 5m (Fig. 1-⑨), inserting the knife block into the hole (Fig. 1-⑩), use a hammer to screw the screws of the manufactured seaweed grafting structure and fixed to natural bedrock (Fig. 1-⑪), and at this time, Ecklonia cava and H. japonica were crossed in a 1:1 ratio and transplanted, and each seaweed was fixed at intervals of less than 20 cm (Fig. 1-⑫).

⑤ The number of transplanted seaweeds at one-month intervals and changes in the length of the fronds were monitored ( FIGS. 2 and 3 ).

Example 2) Structure for transplantation based on natural stone or concrete panel attachment substrate

For Sargassum siliquastrum , two types of concrete panel and natural stone were selected to compare the effect of attaching seaweed according to the type of substrate. For this purpose, an attachment substrate having a weight of 10 to 30Kg was selected.

Twist cap ( Sargassum siliquastrum ) The structure for transplantation was prepared as follows ( FIG. 4 ).

① Four natural stone and 25cm x 40cm x 5cm concrete panels with a diameter of about 50cm were prepared (Figs. 1-①, ②).

② In order to compare the effects of the method of attaching seaweed directly to the substrate with an instant adhesive and the method of attaching via an epoxy resin, an epoxy resin was attached to a natural stone and concrete panel with a diameter of about 3 cm (Figs. 4-① and Fig. 4). -②)

③ After removing foreign substances from the pretzel hat attachment device, the attachment site was smoothed with a sharp knife (Fig. 4-③), and moisture was removed from the attachment area using a moisture absorbent paper or an air gun.

When using the air gun, the moisture was removed by treating air at 4 to 20° C. for 10 to 60 seconds in the attachment machine (FIG. 4-④). For moisture removal, the treatment time was different depending on the ambient temperature, but the treatment was usually performed within 60 seconds.

④ Instantaneous adhesive was applied to the attachment device of the twist hat and fixed to the predetermined attachment site (Figure 4-①, ②) of the attachment substrate (Figure 4-⑥). Nine twisted hats were attached to one attachment substrate, and the distance between each twisted hat was about 15cm.

A graft structure was prepared by attaching 18 direct-attached seaweeds and 18 epoxy resin-mediated seaweeds to four natural stone attachment substrates, and concrete panels were also manufactured in the same way (Figs. 4-⑦, ⑧).

That is, 36 individuals were attached to each of the concrete panel and natural stone, and a total of 72 twisted hats were attached.

⑤ The completed structure for transplantation of seaweed was installed and monitored in the subtidal zone within 2 m of the water depth of the transplant site selected above (Fig. 4-⑨,⑩,⑪,⑫).

Example 3) Stone attachment substrate-based structure for transplantation (Seokbujak)

In the case of Sargassum fulvellum , a relatively light stone with a weight of 1 to 3 kg was selected as an attachment substrate because the risk of overturning or loss by the waves of the test paper was very small.

Sargassum fulvellum ) A structure for transplantation of seaweed attachment (Seokbujak) was prepared as follows (FIG. 5).

① 50 stones with a diameter of about 20 cm were prepared (Fig. 5-①).

② After removing the foreign substances from the staff jaban attachment device, moisture was removed from the attachment site using a moisture absorbent paper or an air gun (FIG. 5-③)

③ After applying the instant adhesive to the staff jaban attachment machine, it was directly attached to the attachment substrate (Figs. 5-④, ⑤)

④ One stele is attached to each attachment substrate, and a total of 50 structures for transplantation of seaweeds were manufactured (FIG. 5-⑥).

⑤ The fabricated structure for transplantation of seaweed (Seokbujak) was thrown from land to the sea at the selected transplant site to test the adhesion strength (FIG. 5-⑦).

⑥ Among the structures for transplanting algae thrown into the water (seokbujak), the number of missing fronds was recorded and collected in one place for easy monitoring in the future (Fig. 5-⑧), and the growth pattern and number of surviving individuals were measured after 3 months of each month. (FIG. 5-⑨).

Comparative Example 1) Structure for implantation bonded with epoxy resin

Adhesive use to the entire attaching machine (rhizome) of the large berry cap transplantation project (bid notice number 20200512254-00) presented by the Jeju Special Self-Governing Province Institute of Oceans and Fisheries for a test of seaweed plantation in the village fishery in Chuja Sea area (Bid Notice No. 20200512254-00) After applying epoxy resin to the cement block and attaching it to the cement block, the cement block was fixed with a wire and dropped into the sea to transplant seaweeds and monitor their survival.

6. Seaweed Monitoring After Construct Implantation

The transplanted seaweed is assigned a unique identification number for each seaweed individual, and the survival rate is investigated in the water by substrate (hardened button-type epoxy resin, natural stone, concrete panel and stone) and attachment method (direct attachment, epoxy resin-mediated attachment). did.

1) Survival rate and growth pattern after underwater transplantation of a button-type attachment matrix-based transplantation structure

Using the natural bedrock transplantation structure for each seaweed individual, 33 Ecklonia cava and 28 Sargassum macrocarpum were transplanted, and monthly monitoring was performed on them.

One month after transplantation, 8 Ecklonia cava and 5 Sargassum macrocarpum , 4 additional Ecklonia cava and 3 Ecklonia cava dropped out 2 months later, indicating a high dropout rate at the early stage of transplantation. After that, Ecklonia cava dropped out by one individual each 3 months and 6 months after transplantation, but no more dropped off subjects from H. After the final 6 months, 22 individuals survived Ecklonia genitalia, with a survival rate of 66.7%, and 20 individuals of Ecklonia cava survived and recorded a survival rate of 71.4%, and the overall survival rate was 68.9% (Figs. 3-①, ②). ).

The average leaf length of E. Ecklonia was 23.1 cm at the time of transplantation, but it showed a tendency to slightly decrease to 15.6 cm one month after transplantation. In the case of large berry hat spots, at 12.9 cm at the time of transplantation, it showed a low growth rate of less than 1 cm for 2 months, but showed a rapid growth of about 5 cm from March, 3 months after transplantation (Figs. 3-③, ④)

2) Analysis of survival rate after transplantation of structures for transplantation based on natural stone or concrete panel attachment substrate

The structure for transplantation of Sargassum siliquastrum based on natural stone or concrete panel attachment substrate was transplanted in water on October 12, 2018. In the process of transporting seaweed-attached substrates in the water, one seaweed attached directly from the concrete panel substrate and one attached seaweed mediated by epoxy resin from the natural stone substrate fell off, so a total of 70 seaweeds (35 concrete panels, 35 natural stones) of the monthly survival rates were monitored. In February 2019 monitoring, one concrete panel was lost and one overturned, and two natural stone substrates were found overturned, so further monitoring was stopped.

2-1) Comparison of Survival Rates of Transplanted Twist Caps by Types of Adhesive Substrates

As a result of comparative analysis of survival rates according to the type of adherent substrate, for natural stone substrates, 35 specimens (100%) in October 2018, 32 specimens (91.4%) in November, 28 specimens (80.0%) in December, and 28 specimens in January 2019. (80.0%) and February 16 (80.0%) survived, and in the concrete panel substrate, 35 (100%) in October 2018, 33 (94.3%) in November, 32 (91.4%) in December, In January 2019, 31 specimens (88.6%) and February 17 specimens (45.7%) were observed to survive, indicating that the survival rate of transplanted algae was slightly higher than that of natural stone in concrete panels. Up to 3 months after attachment, the survival rate of transplanted seaweeds was more than 80% higher in both types of transplantation substrates.

2-1) Comparison of Survival Rates of Transplanted Twisted Capsules by Attachment Method

When comparing the survival rates of adherent seaweeds according to the seaweed attachment method, the number of seaweeds directly attached to the substrate with an instant adhesive was 35 in October 2018 (100%), 33 in November (94.3%), and 29 in December. (82.9%), 29 specimens (82.9%) and 19 specimens (54.3%) in February 2019 survived. In October 2018, 35 specimens (100%) ), 32 specimens (91.4%) in November, 31 specimens (88.6%) in December, 30 specimens (85.7%) in January 2019, and 14 specimens (40.0%) in February 2019. There was no significant difference between groups.

3) Analysis of survival rate after transplantation of stone-attached matrix structures

The structure for transplantation of purpura based on the stone attachment substrate was transplanted underwater on January 4, 2017, and in the process of throwing the fabricated purpura ( Sargassum fulvellum ) transplantation structure, 4 objects fell out, so a total of 46 individuals were targeted. Monthly survival rates were monitored.

In addition, monitoring was stopped in May 2017, when the staff purpura formed the genitalia and became completely mature.

As a result of monitoring, 4 additional specimens were eliminated in February 2017, and 40 individuals (87.0%) survived. In March, 4 additional specimens were dropped, and 36 specimens (78.3%) survived. It is judged that this is due to the eating damage of the conch, which is a breakfast animal, and all the conch around the staff purpura transplant site were removed. In April, 36 specimens (78.3%) were confirmed to have survived while the March population remained the same, and most of the transplanted rhizomes formed a reproductive deposit for sexual reproduction.

4) Analysis of survival rate after transplantation of the structure for transplantation bonded with the epoxy resin of Comparative Example 1

As a result of observing the growth after the underwater transplantation of Comparative Example 1, it was dropped before the epoxy resin was completely cured, and the entire frond along with the epoxy resin fell off, or the attachment phase part coated with the epoxy resin was necrotic due to lack of photosynthesis and cellular respiration. It was confirmed that the growth was not maintained after 2 months after transplantation (FIG. 6).

100: Epoxy resin cured by molding in the form of a button
200: drilling for screw insertion
300: Knife block inserted into the natural rock perforation
400: Epoxy resin-mediated seaweed attachment point
500: seaweed direct attachment point

Claims (9)

A structure for transplanting seaweed comprising perennial Sargassaceae seaweed, an adherent substrate, and a screw,
The perennial mother and algae have a frond length of 10 to 30 cm,
The attachment substrate is in the form of a button made of a cured epoxy resin, and has one or more holes formed therein;
The screw is inserted into the hole of the attachment substrate,
The button-shaped attachment substrate is a polygonal or circular shape having a width or diameter of less than 3 cm to 6 cm, and a thickness of 0.8 to 1.2 cm,
The structure for transplanting seaweed will be fixed in the perforation of the bedrock together with the knife block by the screw,
Structures for transplanting seaweed.
delete delete delete delete delete Preparing a perennial Sargassaceae seaweed and an adherent substrate having a frond length of 10 to 30 cm; and
fixing the perennial Sargassaceae seaweed attachment group (rhizome) to the surface of the attachment substrate with an instant adhesive;
A method of manufacturing a structure for transplanting seaweed comprising:
In the step of preparing the adhesion substrate, a semi-solid epoxy resin and a curing agent are mixed, and the width or diameter is 3 cm to 6 cm, and after molding into a polygonal or circular button shape with a thickness of 0.8 to 1.2 cm, one corner Drilling a hole and hardening
The attachment substrate further comprises a screw inserted into the hole,
The structure for transplanting seaweed will be fixed in the perforation of the bedrock together with the knife block by the screw,
A method of manufacturing a structure for transplanting seaweed.
delete Inserting the knife block after drilling in the natural rock; and
Fixing the structure for transplanting seaweed of claim 1 to the perforation together with the knife block using a screw;
A method of transplanting a structure for transplanting seaweed comprising a.

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