KR102266758B1 - Automatic sample pretreatment apparatus for the sample analysis - Google Patents

Abstract

The present invention relates to a pre-processing automation apparatus for sample analysis and a sample pre-processing method using the same, and more particularly, to a base frame, a washing tray unit sequentially disposed on the base frame in a left and right direction, a sample tray unit, a filter tray unit, and a vial tray A needle unit provided to be slidable in the left and right and up and down directions on a filtration unit including a unit and a filter detachment unit, and a needle unit provided to accommodate overlap in the filtration unit and slidably provided with the needle unit, the needle By including an actuator for pumping and discharging the sample through the unit to enable suction and discharge, it provides the advantage of reducing labor by automating all of the conventional pre-processing process performed manually by the tester.

Description

AUTOMATIC SAMPLE PRETREATMENT APPARATUS FOR THE SAMPLE ANALYSIS

The present invention relates to an automated pre-processing apparatus for sample analysis, and more particularly, to an automated pre-processing apparatus for sample analysis that automates a pressure-type filtration process during a pre-treatment process for sample analysis of a tablet product of a pharmaceutical company.

Generally, tablet products manufactured by pharmaceutical companies are analyzed through sample analysis to determine whether they satisfy applicable laws and regulations.

1 is a flowchart illustrating a typical sample analysis process.

Sample analysis, as shown in Figure 1, after the tablet product sample input and weight check (S1), after pulverizing the sample (S2), through the solvent injection and dilution process (S3), stirring extraction ( After performing S4) and ultrasonic extraction (S5), centrifugation (S6), filtration and vial injection (S7) are performed through various processes, and then sample analysis is performed through HPLC, UPLC, etc.

However, in the above filtration and vial injection process (S7), the tester's labor intensity is high as the tester manually filtered through the syringe filter.

In addition, due to the manual operation of the filtration and vial injection process (S7) as described above, the test time was greatly increased, which took a lot of time for the overall sample analysis.

The present invention has been devised to solve the above technical problem, and it is an object of the present invention to provide a preprocessing automation device for sample analysis that can reduce labor time and improve consumer accessibility by manufacturing as a low-cost low-cost model .

A pre-processing automation apparatus for sample analysis according to an embodiment of the present invention includes a base frame, a washing tray unit sequentially arranged in a left and right direction on the base frame, a sample tray unit, a filter tray unit, a vial tray unit, and a filter detachment unit A needle unit provided slidably in the left and right and up and down directions on a filtration unit comprising a needle unit and a needle unit provided to accommodate overlap in the filtration unit and provided slidably with the needle unit, the sample through the needle unit It may include an actuator for pumping and discharging suction and discharge.

Here, it is provided on the base frame, it may further include a guide frame for guiding the sliding movement of the needle unit and the actuator in the left-right direction and the vertical direction.

In addition, the guide frame includes a pair of vertical frames extending vertically upwardly on the base frame and spaced apart from each other by a predetermined distance and a connection frame connecting the upper ends of the vertical frames horizontally to each other, the connection frame includes , A guide rail groove may be machined into a groove shape to guide the movement of the sliding housing to which the needle unit and the actuator are coupled.

In addition, the needle unit and the actuator may be connected to communicate with each other by a flexible connection tube through which the sample can flow.

In addition, the needle unit is provided with a plurality of individual needles extending downward, and the needle unit and the actuator have a plurality of control valves for independently controlling the flow of the sample flowing through the plurality of individual needles of the needle unit. It may be connected in communication with each other through the provided valve end.

In addition, the needle unit protrudes downward, and includes a plurality of individual needles arranged before and after the base frame, and the plurality of individual needles are accommodated in overlap at the same time in an operation of moving downward in the washing tray, so that suction and discharge are possible. Possible wash water can be accommodated.

In addition, the sample tray unit includes test tubes arranged in front and back of the base frame to correspond to the number of the plurality of individual needles, and arranged in a plurality of rows in the left and right direction of the base frame, and the sample is accommodated in a predetermined amount in the test tube. can be

In addition, in the discharge tray unit, the plurality of individual needles are accommodated at the same time overlapped by an operation of moving downward, and the sample sucked into the plurality of individual needles may be stored when some of the samples are discharged.

In addition, the filter tray unit is arranged before and after the base frame to correspond to the number of the plurality of individual needles, and a plurality of syringe filters mounted on a plurality of filter mounting units arranged in a plurality of rows in the left and right direction of the base frame. Including, after being combined with the plurality of syringe filters provided in the same row among the plurality of filter mounting units in an operation in which the plurality of individual needles are moved downward, the plurality of individual needles are moved upward by an operation in which the plurality of individual needles are moved upward. It may be moved upward together with the plurality of syringe filters provided in the same row among the filter mounting units.

In addition, the vial tray unit is arranged in front and back of the base frame to correspond to the number of the plurality of individual needles, a plurality of vial injection holes arranged in a plurality of rows in the left and right direction of the base frame, and the plurality of vial injection holes It includes a plurality of vial containers respectively disposed in a corresponding lower portion, and in the plurality of vial injection holes, after a syringe filter mounted on each of the plurality of individual needles from the filter tray unit is seated, the needle unit or the After the sample is filtered through the syringe filter by the pumping operation of the actuator, it may be respectively injected into the plurality of vial containers.

In addition, the filter detachment unit, a support panel protruding upward higher than the upper end of the vial tray unit, and a triangular groove corresponding to the number of the plurality of individual needles at the front end extending horizontally from the support panel toward the vial tray unit Including the detachable panel formed, the syringe filter individually mounted from the filter tray unit to each of the plurality of individual needles is caught in the lower part of the triangular groove formed in the detachable panel by an operation in which the plurality of individual needles are moved upward. and can be detached.

An embodiment of the sample pretreatment method using the automated pretreatment apparatus for sample analysis according to the present invention includes a sample pipetting step of pipetting a quantitative sample from a plurality of test tubes provided in the sample tray unit using a washed needle unit; An initial discharge step of initially discharging the sample to the discharge tray unit after the sample pipetting step, a filter mounting step of mounting a syringe filter from the filter tray unit to the needle unit from the filter tray unit after the initial discharging step, and the loading of the filter after the filter mounting step After the filtering step of discharging the sample to the vial container by moving the needle unit equipped with a paper filter to the vial tray unit and the filtering step is completed, the syringe filter mounted on the needle unit is detached from the detachable filter. The sample pipetting step, the initial discharge step, the filter mounting step, the filtering step, and the filter removal step include the steps of: the sample tray unit, the discharge tray unit, the filter tray unit, the vial tray unit and the removal The pumping operation is performed while moving the needle unit in the left and right directions and up and down directions on the filtration unit including the part.

Here, the method may further include a needle washing step of washing the needle unit before the sample pipetting step.

In addition, the filtration unit is sequentially arranged in the left and right directions on the base frame, and the needle unit is provided with a plurality of individual needles extending downward and spaced apart from each other in the front-rear direction, wherein the sample pipetting step includes: It may be a step of pipetting a quantitative amount from the sample accommodated in a plurality of test tubes provided in the sample tray unit with a number corresponding to the plurality of individual needles of the unit.

In addition, the initial discharge step may be a step of discharging a predetermined amount of the sample sucked into the needle unit by the sample pipetting step to the discharge tray unit.

In addition, the filter mounting step is an operation of moving the needle unit downward, and as the filter tray unit, a plurality of rows are arranged in the left and right direction of the base frame so as to correspond to the number of the plurality of individual needles. It may be a step of mounting a plurality of syringe filters mounted on a plurality of filter mounting units to a plurality of individual needles of the needle unit.

In addition, the filtering step is an operation of moving the needle unit in a state in which the plurality of syringe filters are mounted on the tip of the plurality of individual needles of the needle unit downward as the vial tray unit of the plurality of individual needles. Doedoe arranged in front and back of the base frame to correspond to the number, through a plurality of vial injection holes arranged in a plurality of rows in the left and right direction of the base frame, a plurality of vial containers respectively disposed in the lower part corresponding to the plurality of vial injection holes. It may be an injection step.

In addition, in the filter detachment step, the plurality of syringe filters mounted on the front end of the plurality of individual needles of the needle unit as the filter detachment unit are the vial tray from the support panel protruding upward higher than the upper end of the vial tray unit. It may be a step of detaching by an operation that interferes with the detachable panel extending horizontally to the unit side and having a triangular groove corresponding to the number of the plurality of individual needles at the tip.

According to an embodiment of the automated preprocessing apparatus for sample analysis according to the present invention, it is possible to achieve the effect of improving test efficiency by shortening the overall sample analysis time as well as improving work efficiency by reducing the labor time of the tester. can

1 is a flowchart showing a typical sample analysis process;
2 is a flowchart showing the detailed process of the filtration and vial injection process using the automated pre-processing apparatus for sample analysis according to an embodiment of the present invention;
3 is a perspective view showing an automated pre-processing apparatus for sample analysis according to an embodiment of the present invention;
Figures 4a and 4b are a front view and a plan view of Figure 3,
5 is a perspective view showing the coupling of the actuator and the needle unit in the configuration of FIG. 3 and a cut-away perspective view taken along line AA;
Figure 6 is a perspective view showing the needle unit in the configuration of Figure 3,
7 is a cutaway perspective view and a partially enlarged view taken along line BB of FIG. 6 ,
8a to 8f are front views showing a specific appearance of the filtration and vial injection process of FIG. 2,
Figure 9 is a front view showing the mounting state of the syringe filter according to Figure 8d,
10A and 10B are perspective views illustrating an exploded state of the syringe filter according to FIG. 8F.

Hereinafter, an embodiment of an automated preprocessing apparatus for sample analysis according to the present invention will be described in detail with reference to the accompanying drawings. In adding reference numerals to the components of each drawing, it should be noted that the same components have the same sub-hols as much as possible even though they are indicated on different drawings. In addition, in describing the embodiment of the present invention, if it is determined that a detailed description of a related known configuration or function interferes with the understanding of the embodiment of the present invention, the detailed description thereof will be omitted.

In describing the components of the embodiment of the present invention, terms such as first, second, A, B, (a), (b), etc. may be used. These terms are intended to distinguish the component from other components, and the essence, order, or order of the component is not limited by the term. In addition, unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Terms such as those defined in a commonly used dictionary should be interpreted as having a meaning consistent with the meaning in the context of the related art, and should not be interpreted in an ideal or excessively formal meaning unless explicitly defined in the present application. does not

2 is a flowchart illustrating a detailed process of a filtration and vial injection process using an automated preprocessing apparatus for sample analysis according to an embodiment of the present invention, and FIG. 3 is an automated preprocessing apparatus for sample analysis according to an embodiment of the present invention. is a perspective view showing a, and FIGS. 4A and 4B are a front view and a plan view of FIG. 3 .

Prior to describing the automated pre-processing apparatus 1 for sample analysis according to an embodiment of the present invention in detail, the filtration and vial injection process of the sample pre-processing process using the automated pre-processing apparatus 1 for sample analysis according to the present invention Let's look at it in detail.

2, the filtration and vial injection process is a needle washing step (S60) of moving the needle unit 40 to the initial washing tray to wash the plurality of individual needles 43 of the needle unit 40, and washing A sample pipetting step (S10) of pipetting a quantitative sample using the needle unit (40), an initial discharge step (S20) of initially discharging the pipetted sample to a discharge tray (S20), and a syringe filter (401) A filter mounting step (S30) of mounting the needle to the lower end of the needle of the needle unit (40), a filtering step (S40) of filtering the sample in the discharge tray by moving it to the vial tray and discharging it, and a syringe filter (401) after filtering ) may include a filter detachment step (S50) of desorbing, and a needle washing step (S60) of washing the needle unit 40 after the filter detachment step (S50).

Here, when the needle unit 40 is used for the first time or the needle washing step (S60) has already been performed, the filtration and vial injection process begins with the first sample pipetting step (S10), and the needle washing step (S60) may end in The detailed process from the sample pipetting step (S10) to the needle washing step (S60) described above will be described in detail after examining the specific configuration of the automated preprocessing apparatus 1 for sample analysis according to an embodiment of the present invention. do it with

Referring to FIGS. 3 to 10B , the automated preprocessing apparatus 1 for sample analysis according to an embodiment of the present invention may include a base frame 10 and a needle unit 40 moving on the filtration unit. have.

The base frame 10 is a frame such that the filtration and vial injection processes, which are sample pretreatment processes, are performed as a whole, and may have a horizontal table shape.

The filtration unit includes a washing tray unit 100, a sample tray unit 200, a filter tray unit 400, a vial tray unit 500, and a filter detachment unit ( 600) may be provided.

The needle unit 40 may be slid left and right and up and down on the filtration unit. The needle unit 40 may be accommodated in an overlapping filtration unit.

The needle unit 40 may be provided with a plurality of individual needles 43 spaced apart by a predetermined distance in the front-rear direction of the base frame 10 to enable filtration and vial injection of a plurality of unit samples. The plurality of individual needles 43 are a kind of needle-shaped nozzle, which serves to suck/discharge a liquid fluid (this is a concept including washing water and a sample to be described later) to the needle unit 40 as a filtration unit. is the part The specific configuration and coupling relationship of the needle unit 40 will be described later.

Hereinafter, the units provided in the filtration unit will be described.

Among the filtration units, the washing tray unit 100 is provided in the form of a container with an open top on the base, and in the front and rear direction so that the washing water is simultaneously absorbed/discharged through all of the plurality of individual needles 43 of the needle unit 40 . It may be provided as a washing water storage container formed long.

In the washing tray unit 100, a predetermined amount of washing water for washing the needle unit 40 in order to minimize the chemical influence and external influence on the analyte sample when it is filtered and injected into the vial using the needle unit 40 can be accepted.

Among the filtration units, the sample tray unit 200 may have a plurality of test tubes 201 mounted upright on a test tube mounting panel having a plurality of mounting holes to be opened in the vertical direction. A predetermined amount of a sample to be analyzed may be accommodated in the plurality of test tubes 201 . The plurality of test tubes 201 may be accommodated to overlap the plurality of individual needles 43 of the needle unit 40 downward to suck and store the sample in a pumping operation.

Among the filtration units, the discharge tray unit 300 is provided in the form of a container with an open top on the base, similar to the wash water storage container of the above-described wash water unit, and simultaneously passes through all of the plurality of individual needles 43 of the needle unit 40 . It may be provided as a discharge sample storage container formed to be elongated in the front-rear direction so that a predetermined amount of the sample inhaled and stored from the sample tray unit 200 is discharged.

As such, the reason for initially discharging a predetermined amount of the sample from the plurality of individual needles 43 of the needle unit 40 to the discharge sample storage container of the discharge tray unit 300 is present at the initial inlet of the plurality of individual needles 43 to remove air bubbles.

Among the filtration units, the filter tray unit 400 includes a filter mounting panel in which a plurality of filter mounting parts are formed so as to be formed in a groove shape that is opened in the vertical direction or recessed downward, and a plurality of syringe filters 401 are mounted in a plurality of filters. Can be mounted on each part. A plurality of syringe filters 401 are mounted on the tip of a plurality of individual needles 43 of the needle unit 40, and the sample inhaled and stored in the needle unit 40 is injected into a vial container 502 to be described later while filtering it. can do.

Of the filtration units, the vial tray unit 500 is arranged before and after the base frame 10 so as to correspond to the number of a plurality of individual needles 43, and a plurality of vial injection holes arranged in multiple rows in the left and right direction of the base frame 10 ( It may include a guide panel 501 is formed, and a plurality of vial containers 502 respectively disposed at the lower portion corresponding to the plurality of vial injection holes (501).

The vial tray unit 500 is a state in which the syringe filter 401 mounted on the tip of a plurality of individual needles 43 of the needle unit 40 through the filter tray unit 400 is seated in the vial injection hole 501 . In the operation of discharging the sample inhaled and stored in the needle unit 40, it can serve to filter and inject the sample into the vial container 502 at the same time.

The filter detachment unit 600 is a syringe filter 401 mounted on the tip of a number of individual needles 43 of the needle unit 40 in a state where filtration (filtering) and vial injection in the vial tray unit 500 are completed. It may play a role in desorption.

More specifically, the filter detachment unit 600 extends horizontally from the support panel 601 upwardly protruding higher than the upper end of the vial tray unit 500 to the vial tray unit 500 side from the support panel 601 and , It may include a detachable panel 602 having a triangular groove 603 corresponding to the number of the plurality of individual needles 43 formed at the tip.

Here, the triangular groove 603, the vial tray unit 500 side is cut so that the middle portion of the plurality of individual needles 43 of the needle unit 40 horizontally moved from the vial tray unit 500 side can be accommodated. can be formed.

The syringe filter 401 individually mounted from the filter tray unit 400 to each of the plurality of individual needles 43 moves in the horizontal direction when filtering and vial injection in the vial tray unit 500 are completed, as described above. In a state where the syringe filter 401 is positioned on the lower side of the triangular groove 603, the plurality of individual needles 43 are caught in the lower part of the triangular groove 603 formed in the detachable panel 602 by an upward movement. and can be detached.

In one embodiment of the present invention, the needle unit 40, as described above, the wash water tray unit, the sample tray unit 200, the discharge tray unit ( 300), the filter tray unit 400, the vial tray unit 500, and the filter detachment unit 600 are moved in the left and right directions or moved in the vertical direction from the top of the pretreatment for sample analysis according to an embodiment of the present invention to be described later A pre-processing method using the automated device 1 may be performed.

To enable such a pretreatment method, the automated preprocessing apparatus 1 for sample analysis according to an embodiment of the present invention is provided to be slidable together with the needle unit 40 , and absorbs the sample through the needle unit 40 . An actuator 50 for pumping and discharging to be discharged, and a guide frame provided on the base frame 10 and guiding the sliding movement in the left and right and up and down directions of the needle unit 40 and the actuator 50 can do.

3 to 4b, the guide frame extends vertically upward on the base frame 10 and is a pair of vertical frames 21 and 22 spaced apart from each other by a predetermined distance, and the vertical frames 21 and 22 It may include a connection frame 23 for horizontally connecting the upper end to each other.

The connection frame 23 may include a guide rail groove 24 to guide the movement of the sliding housing 30 to which the needle unit 40 and the actuator 50 are coupled. The guide rail groove 24 may be formed in a groove shape in the connection frame 23 .

The needle unit 40 and the actuator 50 may be moved in the left and right directions from the top on the base along the guide rail groove 24 in a state inserted and accommodated in the sliding housing 30 . Among the needle units 40 , the above-described plurality of individual needles 43 are formed to protrude a predetermined distance to the lower portion of the sliding housing 30 to perform a pretreatment method.

Meanwhile, although not shown in the drawing, the automated preprocessing apparatus 1 for sample analysis according to an embodiment of the present invention slides the sliding housing 30 in the left and right directions along the guide rail groove 24, or up and down It may further include a sliding driving unit (refer to reference numeral 60 in FIG. 3) for sliding movement in the direction. However, the sliding driving unit 60 is not necessarily provided, and the sliding movement of the sliding housing 30 in the left and right direction or in the vertical direction may be possible by manual operation by a tester.

Figure 5 is a perspective view showing the coupling of the actuator and the needle unit in the configuration of Figure 3 and a cut-away perspective view taken along line AA, Figure 6 is a perspective view showing the needle unit in the configuration of Figure 3, Figure 7 is BB of Figure 6 It is a cut-away perspective view and some enlarged views taken along a line.

5 to 7, the needle unit 40 and the actuator 50 inserted and accommodated in the sliding housing 30 will be described in more detail.

Referring to FIG. 5 , the actuator 50 may include a motor unit 51 , a pumping unit 53 , a piston rod 82 , a piston unit 84 , and a restoration member 85 .

The motor unit 51 may be disposed on one side of the pump installation bracket that mediates the coupling to the inside of the sliding housing 30 .

The pumping unit 53 may be disposed on one side of the motor unit 51 to pump the fluid.

The piston rod 82 may be connected to the motor rotation shaft 81 provided on one side of the motor unit 51 to move in the axial direction toward the pumping unit 53 .

The piston part 84 may be disposed at the front end of the piston rod 82 disposed on the pumping part 53 side.

The restoration member 85 may be disposed in the inner space of the pumping unit 53 to restore the piston unit 84 to its original state.

The actuator 50 having such a configuration may be connected to each other in communication with the needle unit 40 and the flexible connection tube 49 through which the sample can flow. However, it is not necessary for the sample to flow in the flexible connection tube 49 itself, for example, a gas such as air is provided so that the inside of the pumping part 53 of the actuator 50 and the needle unit 40 communicate with each other and flow, The sample may be sucked/discharged into the needle unit 40 by the movement of the piston of the needle unit 40 to be described later by the change in air pressure.

6 and 7 , the needle unit 40 may include a valve part 72 , a piston part 79 , and a cylinder part 73 .

The valve part 72 may be disposed on the top of the needle installation bracket 41 that mediates the coupling to the inside of the sliding housing 30 .

The piston part 79 may be disposed in communication with the plurality of individual needles 43 via the valve tube 45 at the lower part of the valve part 72 . The piston part 79 may reciprocate by the piston rod 76 .

The cylinder part 73 may have a sample receiving chamber 78 for accommodating the sample therein.

The valve part 72 and the cylinder part 73 may be provided integrally or may be connected via the valve tube 45 .

The valve part 72 is provided on each of the plurality of individual needles 43 so that integrated control by the actuator 50 is possible, while individual control may be possible in units of individual needles 43 . That is, when some of the plurality of individual needles 43 required for testing are some, only some individual needles 43 perform on/off control of the valve unit 72 so that the sample can be suctioned/discharged, and the remaining individual needles 43 are The needle 43 can be individually controlled by turning it off.

For example, referring to FIG. 4 , when the sample tray unit 200 is provided with five test tubes 201 in the front and rear directions, and analysis samples of different types of tablet products are required for each of the five test tubes 201 . , it is necessary to control the intake/discharge of the needle unit 40 in units of individual needles 43 . Therefore, it is preferable that the valve unit is arranged to enable individual intermittent control of the individual needle 43 units. For example, if five individual needles 43 are provided, the valve unit is provided with five individual needles 43 may be independently intercepted.

Hereinafter, an embodiment of a sample pretreatment method using the automatic pretreatment apparatus 1 for sample analysis according to the present invention configured as described above will be described in detail with reference to the accompanying drawings.

8A to 8F are front views showing a specific state of the filtration and vial injection process of FIG. 2 , FIG. 9 is a front view showing the mounting state of the syringe filter according to FIG. 8D, and FIGS. 10A and 10B are according to FIG. 8F It is a perspective view showing the disassembled state of the syringe filter.

A sample pre-processing method (hereinafter, abbreviated as 'pre-processing method according to this embodiment') using the automated pre-processing apparatus 1 for sample analysis according to an embodiment of the present invention is shown in FIGS. 2 and 8A to 10B . As described above, a sample pipetting step (S10) of pipetting a quantitative sample from a plurality of test tubes 201 provided in the sample tray unit 200 using the washed needle unit 40, a sample pipetting step ( After the initial discharge step (S20) of initially discharging the sample to the discharge tray unit 300 after S10), the syringe filter 401 is mounted on the needle unit 40 from the filter tray unit 400 after the initial discharge step (S20) After the filter mounting step (S30), the filter mounting step (S30), the needle unit 40 equipped with the syringe filter 401 is moved to the vial tray unit 500 to discharge the sample into the vial container 502. Filtering After the step (S40) and the filtering step (S40) are completed, it may include a filter detachment step (S50) of detaching the syringe filter 401 mounted on the needle unit 40 from the detachable unit.

If the needle unit 40 used before the sample pipetting step (S10) is for initial use, it is not necessary to separately wash the needle unit 40, but in an embodiment of the present invention, before the sample pipetting step (S10) It may be provided to further include a needle washing step (S60) of washing the needle unit (40).

Referring to Figure 8a, the needle washing step (S60) by sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 from directly above the washing tray unit 100 to the downward direction of the needle unit ( After allowing the lower ends of the plurality of individual needles 43 of 40) to be immersed in the washing water, the washing water absorption/discharge operation by the actuator 50 may be performed several times.

The needle unit 40 is moved from the lower part of the washing tray unit 100 to a directly upward direction in order to perform the next sample pipetting step (S10) and then slid in the right direction directly above the sample tray unit 200. can be stopped At this time, a process of drying the inside of the needle unit 40 may be further performed by using the actuator 50 to suck/discharge air several times to the needle unit 40 .

Referring to FIG. 8B , the sample pipetting step ( S10 ) is a step of pipetting a sample to be analyzed contained in a plurality of test tubes 201 disposed in the sample tray unit 200 , and includes a needle unit 40 and an actuator 50 . ) by sliding the sliding housing 30 provided with the sample tray unit 200 downward from directly above the needle unit 40 so that the lower ends of the plurality of individual needles 43 of the needle unit 40 are immersed in the sample, and then the actuator 50 ) can be performed by performing a quantitative suction operation by

The needle unit 40 is moved directly above the sample tray unit 200 to perform the next initial discharge step (S20), and then is slid in the right direction directly above the discharge tray unit 300 to be stopped. have. In this case, in order to prevent the sample from being lost during transport, the needle unit 40 may be shaved and a time delay process may be further performed.

Referring to Figure 8c, the initial discharge step (S20) by sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 downward from directly above the discharge tray unit 300, the discharge tray unit A part of the sample sucked into the needle unit 40 may be discharged to the discharge sample storage container of 300 . Such an initial discharge step (S20) is to remove air bubbles at the inlet of a plurality of individual needles 43 of the needle unit 40 and to inject only a quantitative sample when later injected into the vial container 502 can play a role

The needle unit 40 is moved directly above the discharge tray unit 300 to perform the next filter mounting step (S30), and then is slid in the right direction directly above the filter tray unit 400 to be stopped. have. At this time, in order to prevent the sample from being lost during transport, a time delay process of the needle unit 40 may be further performed.

Referring to FIG. 8D , the filter mounting step (S30) is performed by sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 downward from the directly above the filter tray unit 400 to move the filter mounting panel The lower end of the plurality of individual needles 43 of the needle unit 40 may be seated on the syringe filter 401 mounted on the plurality of filter mounting units of the.

Referring to FIG. 9 , in a state in which the syringe filter 401 is coupled to the lower end of the plurality of individual needles 43 of the needle unit 40, the filter tray unit 400 for performing the next filtering step (S40) ) can be stopped by sliding in the right direction directly above the vial tray unit 500 after moving to the upper direction. At this time, in order to check whether the syringe filter 401 is correctly mounted on the needle unit 40 during transport, a time delay process may be further performed.

Referring to FIG. 8E , the filtering step (S40) is performed by sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 downward from directly above the vial tray unit 500 to move the vial of the guide panel. After seating the syringe filter 401 with the injection hole 501, the sample is filtered through the filter tray by the discharge operation by the actuator 50, and at the same time, the sample is placed in the vial container 502 provided at the bottom of the guide panel. This is the injection step.

After the needle unit 40 is moved directly above the vial tray unit 500 to perform the next filter removal step (S50), the filter removal unit 600 is slid in the right direction where it is located and can be stopped. .

Referring to FIG. 8f, the filter removal step (S50) is performed by sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 in the right direction from directly above the vial tray unit 500, FIG. As referenced in 10a, in a state in which a plurality of individual needles 43 of the needle unit 40 are respectively received in the triangular grooves 603 formed in the detachable panel 602 of the filter detachment unit 600, FIG. 10b As referenced in, in an operation of sliding the sliding housing 30 provided with the needle unit 40 and the actuator 50 directly upward, the individual needle 43 of the needle unit 40 is coupled to the lower end of the cylinder This is a step of removing the paper filter 401 .

When the filter removal step (S50) is completed, the sliding housing 30 provided with the needle unit 40 and the actuator 50 is slid to a position where the first needle washing step (S60) is performed, and then the next sample pretreatment The process can be repeated.

According to an embodiment of the pre-processing automation apparatus 1 for sample analysis and the sample pre-processing method using the same according to the present invention performed as described above, by automating the manual work process by the tester of the sample pre-processing process before sample analysis of the tablet product It is possible to shorten the working time, as well as improve the accuracy of the pre-processing process.

An embodiment of the automatic pre-processing apparatus for sample analysis and the sample pre-processing method using the same according to the present invention has been described in detail with reference to the accompanying drawings. However, the embodiment of the present invention is not necessarily limited by the above-described embodiment, and it is natural that various modifications and implementations within an equivalent range are possible by those of ordinary skill in the art to which the present invention pertains. will be. Therefore, the true scope of the present invention will be determined by the claims to be described later.

S10: sample pipetting step S20: initial ejection step
S30: filter mounting step S40: filtering step
S50: filter removal step S60: needle washing step
1: Pre-processing automation device 10: Base frame
21,22: vertical frame 23: connecting frame
24: guide rail groove 30: sliding housing
40: needle unit 41: needle mounting bracket
43: individual needle 45: valve tube
49: flexible connecting tube 50: actuator
51: motor unit 53: pumping unit
60: sliding driving unit 72: valve unit
73: cylinder part 76: piston rod
77: restoration member 79: piston unit
81: motor rotation shaft 82: piston rod
84: piston rod 85: restoration member
100: washing tray unit 200: sample tray unit
201: test tube 300: discharge tray unit
400: filter tray unit 401: syringe filter
500: vial tray unit 501: vial injection hole
502: vial container 600: filter removal unit
601: support panel 602: detachable panel
603: triangular groove

Claims (17)

a base frame that is a frame in the form of a horizontal table;
Sliding in the left and right directions and up and down on the filtration unit including the washing tray unit, the sample tray unit, the discharge tray unit, the filter tray unit, the vial tray unit and the filter detachment unit sequentially arranged in the left and right directions on the base frame a needle unit provided so as to be overlapped with the filtration unit;
an actuator provided to be slidable together with the needle unit, and for pumping and discharging the sample so as to be able to suction and discharge the sample through the needle unit; and
A guide frame provided on the base frame and guiding the sliding movement of the needle unit and the actuator in the left and right directions and up and down directions; includes,
The guide frame is
a pair of vertical frames extending vertically upward on the base frame and spaced apart from each other by a predetermined distance; and
and a connection frame for horizontally connecting the upper ends of the vertical frame to each other;
A guide rail groove is formed in a groove shape to guide the movement of the sliding housing in which the needle unit and the actuator are coupled to the connection frame,
The needle unit is provided with a plurality of individual needles extending downwardly,
The needle unit and the actuator are connected in communication with each other through a valve end provided with a plurality of control valves for independently controlling the flow of the sample flowing through a plurality of individual needles of the needle unit,
The washing tray is provided with a washing water storage container in which washing water that can be sucked and discharged is accommodated at the same time as the plurality of individual needles are moved downward.
The discharge tray unit includes a discharge sample storage container in the form of a container with an open top,
The discharge sample storage container is formed to be elongated in the front and rear direction of the base frame so that a predetermined amount of the sample sucked and stored from the sample tray unit is initially discharged through all of the plurality of individual needles at the same time to store the initially discharged sample. a sample storage container;
The filter tray unit is arranged in front and back of the base frame so as to correspond to the number of the plurality of individual needles, and having a plurality of syringe filters mounted on a plurality of filter mounting units arranged in a plurality of rows in the left and right direction of the base frame,
After being combined with the plurality of syringe filters provided in the same row among the plurality of filter mounting units in an operation in which the plurality of individual needles are moved downward, the plurality of filter mounting units is an operation in which the plurality of individual needles are moved upward. is moved upward together with the plurality of syringe filters provided in the same row,
The vial tray unit is arranged before and after the base frame to correspond to the number of the plurality of individual needles, a plurality of vial injection holes arranged in a plurality of rows in the left and right direction of the base frame, and a lower portion corresponding to the plurality of vial injection holes Provided with a plurality of vial containers, each disposed in the
After the syringe filter mounted on each of the plurality of individual needles from the filter tray unit is seated in the plurality of vial injection holes, the sample is transferred to the syringe filter by the pumping operation of the needle unit or the actuator After being filtered through, each injected into the plurality of vial containers,
The filter detachment unit,
a support panel protruding upward higher than the top of the vial tray unit; and
A detachable panel extending horizontally from the support panel toward the vial tray unit and having a triangular groove corresponding to the number of the plurality of individual needles formed at the tip end thereof; and
A syringe filter individually mounted from the filter tray unit to each of the plurality of individual needles is caught and detached from the lower part of the triangular groove formed in the detachable panel by an operation in which the plurality of individual needles are moved upwardly, pre-processing for sample analysis automation device. delete The method of claim 1,
The needle unit and the actuator are connected to each other in communication with each other by a flexible connection tube through which the sample can flow, a pre-processing automation device for sample analysis. delete delete The method of claim 1,
The sample tray unit includes test tubes arranged in a plurality of rows in the left and right direction of the base frame, arranged before and after the base frame so as to correspond to the number of the plurality of individual needles,
In the test tube, the sample is accommodated in a predetermined amount, a pre-processing automation device for sample analysis. delete delete delete delete delete delete delete delete delete delete delete

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